Auxiliary device for separating umbilical vein endothelial cells
By designing an auxiliary device including a guide head, filling capsule and connecting piece, the problem of digestive fluid not being able to fully contact the endothelial cells is solved, and efficient cell harvesting and culture preparation is achieved.
Patent Information
- Application Number
- CN202422217910.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-10
- Publication Date
- 2025-07-25
- Estimated Expiration
- 2034-09-10
AI Technical Summary
In the prior art, when separating umbilical vein endothelial cells, the perfused digestive fluid cannot fully contact the endothelial cells, resulting in too few cells, increasing the difficulty of subsequent culture.
Design an auxiliary device including a guide head, filling capsule and connector, control the quantitative injection of digestive fluid through a syringe, dilate the blood vessels with support tubes, ensure that the digestive fluid fully contacts the cells, and use an umbrella-shaped connector to avoid scratching the cells, and the cannula protects the inside of the blood vessels.
It realizes a fully enclosed state inside the blood vessel, increases the contact area of the digestive fluid, prevents leakage, improves cell harvesting efficiency, and ensures cell integrity and activity.
Smart Images
Figure CN223150539U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of medical device technologies, and particularly to an auxiliary device for separating umbilical vein endothelial cells. Background Art
[0002] Human umbilical vein endothelial cells (HUVEC) are cells isolated from the umbilical vein blood vessels of a full-term delivered fetus and can be cultured in vitro. Since their reactivity to drugs or stimuli is closest to that of the human body and they are relatively easy to obtain, they have become an important tool for basic medical research for many years.
[0003] Currently, the commonly used method for separating umbilical vein endothelial cells is as follows: Flush the lumen of the vein with a buffer solution to thoroughly remove residual blood stains. Then fix both ends of the umbilical vein, perfuse a buffer solution containing collagenase into the vein lumen, tie up the blood vessel opening, and incubate at 37°C for 15 minutes. Finally, collect the digestive solution.
[0004] However, the umbilical vein is an elastic tissue with dense endothelial cells distributed. If the endothelial tissue is not expanded, the amount of perfused digestive solution is insufficient, and the digestive solution cannot fully contact the endothelial cells, resulting in too few cells harvested finally, which increases the difficulty of subsequent cell culture. Utility Model Content
[0005] In order to solve the problem that the digestive solution perfused by the auxiliary device for separating umbilical vein endothelial cells in the prior art cannot fully contact the endothelial cells, resulting in too few cells being obtained, this application provides an auxiliary device for separating umbilical vein endothelial cells.
[0006] The auxiliary device for separating umbilical vein endothelial cells provided by this application adopts the following technical solution:
[0007] An auxiliary device for separating umbilical vein endothelial cells is inserted into the interior of the umbilical vein blood vessel, and includes a guiding head in the shape of a syringe needle and a filling bladder connected to one end of the guiding head and hollow inside. The filling bladder includes a plurality of support tubes distributed circumferentially, and a first connecting member and a second connecting member symmetrically arranged at both ends of the plurality of support tubes. The end of the first connecting member away from the support tube is fixedly connected to the guiding head. The end of the second connecting member away from the support tube is provided with an injection hole through it. An installation ring is arranged at the position of the injection hole at the end of the second connecting member away from the support tube. The inner diameter of the installation ring is equal to the diameter of the injection hole, and the needle of a syringe is sleeved on the installation ring.
[0008] By adopting the above technical solution, the auxiliary device can be completely inserted into the blood vessel, and the syringe is used to control the quantitative injection of digestive fluid into the blood vessel. Due to the elastic and dense characteristics of the blood vessel, it will wrap the auxiliary device, making the inside of the blood vessel in a closed state, so as to better absorb the digestive fluid, solving the problems of insufficient absorption of digestive fluid by the cells inside the blood vessel and liquid leakage during the injection of digestive fluid.
[0009] Optionally, both the first connecting piece and the second connecting piece are umbrella-shaped structures with the same shape and size. The guiding head is fixedly connected to the tip position of the first connecting piece, and the liquid injection hole is opened at the tip position of the second connecting piece.
[0010] By adopting the above technical solution, during the process of the auxiliary device passing through the blood vessel, since the first connecting piece and the second connecting piece are umbrella-shaped structures, it is possible to avoid the first connecting piece and the second connecting piece scratching the cells inside the blood vessel, ensuring the integrity and activity of the cells to the greatest extent, so as to facilitate the later collection and culture of cells.
[0011] Optionally, there are at least three support tubes, and several support tubes are evenly distributed along the circumferential direction of the axis of the guiding head.
[0012] By adopting the above technical solution, the three support tubes evenly distributed along the circumferential direction can form a relatively stable triangular structure, so that the auxiliary device always maintains the stability of the structure during the process of passing through the blood vessel, avoiding being damaged by the elastic pressure on the blood vessel.
[0013] Optionally, the support tube is an arc-shaped structure with bending elasticity and radial elasticity. Several support tubes together enclose an elliptical structure, and the elliptical structure is coaxial with the guiding head.
[0014] By adopting the above technical solution, the elliptical structure is located between the first connecting piece and the second connecting piece, which can make the auxiliary device move more smoothly in the blood vessel. At the same time, it can also be elastically restored under the pressure of the blood vessel to a certain extent, ensuring that the auxiliary device is never damaged.
[0015] Optionally, the diameter of the middle part of the elliptical structure along the direction perpendicular to the axis of the guiding head is larger than the inner diameter of the blood vessel.
[0016] By adopting the above technical solution, the blood vessel can be expanded as much as possible along the radial direction without bursting the blood vessel, increasing the contact area between the inside of the blood vessel and the digestive fluid. At the same time, since the blood vessel is expanded, the elastic force is also greater, further increasing the airtightness of the blood vessel.
[0017] Optionally, sleeves are integrally arranged along the circumferential direction on the inner sides of the first connecting piece and the second connecting piece, and both ends of the support tube are inserted into the inside of the sleeve.
[0018] By adopting the above technical solution, the sleeve being arranged inside the first connecting member and the second connecting member can avoid the situation of scratching blood vessel cells. At the same time, since the support tube is sleeved with the first connecting member and the second connecting member, it is also convenient to disassemble and replace each part structure, improving the utilization rate of the internal parts of the auxiliary device.
[0019] Optionally, the inner diameter of the sleeve is smaller than the outer diameter of the support tube.
[0020] By adopting the above technical solution, the support tube is elastic. When the support tube is connected to the first connecting member and the second connecting member, the support rod elastically abuts against the inside of the sleeve, preventing the support tube from separating from the sleeve during the use of the auxiliary device.
[0021] Optionally, the needle and the mounting ring are in a transition fit of the basic shaft system.
[0022] By adopting the above technical solution, the connection structure between the needle and the mounting ring can be made more stable. The reason for the basic shaft system connection is that the needle is generally a standard part with specifications, and choosing the basic shaft system is more convenient for assembly.
[0023] In summary, the present application includes at least one of the following beneficial technical effects:
[0024] 1. The entire auxiliary device of the present application keeps the umbilical vein in a fully enclosed state, and the venous endothelium has the characteristics of elasticity and compactness. The introduced device can expand the digestion area of the venous endothelium, preventing liquid leakage while improving digestion efficiency;
[0025] 2. The elliptical structure formed by the support tube in the present application enlarges the blood vessel, making the contact area between the inside of the blood vessel and the digestive juice larger, and at the same time increasing the compactness of the blood vessel. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] Figure 1 is an overall structural schematic diagram of an auxiliary device for separating umbilical vein endothelial cells penetrating through a blood vessel.
[0027] Figure 2 is an overall structural schematic diagram of an auxiliary device for separating umbilical vein endothelial cells.
[0028] Figure 3 is Figure 2 an enlarged view of part A in
[0029] Description of the reference numerals: 1, blood vessel; 2, guiding head; 3, filling bladder; 31, elliptical structure; 311, support tube; 32, first connecting member; 33, second connecting member; 331, liquid injection hole; 4, mounting ring; 5, syringe; 51, needle; 6, sleeve. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0030] The following will further elaborate on this application with reference to the accompanying Figures 1-3 drawings for a more detailed description.
[0031] An embodiment of this application discloses an auxiliary device for separating umbilical vein endothelial cells.
[0032] Referring to Figure 1 and Figure 2 , an auxiliary device for separating umbilical vein endothelial cells is inserted into the blood vessel 1 of the umbilical vein to facilitate the separation of umbilical vein cells for separate culture. The auxiliary device includes a guiding head 2 in the shape of a syringe needle, and a filling sac 3 fixedly connected to the guiding head 2. The filling sac 3 specifically includes a number of support tubes 311 distributed circumferentially, and a first connecting member 32 and a second connecting member 33 symmetrically arranged at both ends of the number of support tubes 311. One end of the first connecting member 32 away from the support tube 311 is fixedly connected to the guiding head 2, and a liquid injection hole 331 is formed through the other end of the second connecting member 33 away from the support tube 311. At the position of the liquid injection hole 331, the second connecting member 33 is fixedly installed with an installation ring 4 coaxial with the liquid injection hole 331 and having an inner diameter equal to the diameter of the liquid injection hole 331. The installation ring 4 is detachably sleeved with a needle head 51. The needle head 51 is connected to a syringe 5 for injecting digestive fluid into the blood vessel 1 to ensure cell viability.
[0033] It is worth mentioning that in this application, the needle head 51 and the installation ring 4 are designed with a transition fit on the basis of the basic shaft system. Since the needle head 51 is a standard part with specifications, using the needle head as the mating reference can avoid the situation of specifically searching for a suitable needle head 51. In addition, designing a transition fit between the two is to prevent the separation between the needle head 51 and the installation ring 4 during the operation of the auxiliary device, resulting in injection failure.
[0034] Referring to Figure 2 and Figure 3 , both the first connecting member 32 and the second connecting member 33 are umbrella-shaped structures with the same shape and size, so that the auxiliary device can smoothly slide in the blood vessel 1 and avoid scratching the blood vessel 1 cells. The guiding head 2 is fixedly connected to the tip end of the first connecting member 32, and the liquid injection hole 331 is formed at the tip position of the second connecting member 33. The liquid injection hole 331, the first connecting member 32, the second connecting member 33, and the guiding head 2 are all coaxial.
[0035] Referring to Figure 2 and Figure 3 , there are at least three support tubes 311 to achieve the overall support effect of the auxiliary device. In this design, it is preferred that there are three support tubes 311, and the three support tubes 311 are evenly distributed circumferentially along the guiding head 2. The three support tubes 311 can form a relatively stable triangular structure to play a supporting role for the auxiliary device.
[0036] Referring toFigure 2 Further, the support tube 311 has an arc-shaped structure and has bending elasticity and radial elasticity. The three support tubes 311 together enclose an ellipsoid structure 31, and the diameter of the middle part of the ellipsoid structure 31 along the axial direction is larger than the diameter of the blood vessel 1 in its normal state, so that while expanding the blood vessel 1 to make the inner wall cells of the blood vessel 1 have a larger contact area with the digestive fluid, the elastic extrusion force of the blood vessel 1 on the auxiliary device is also increased, improving the compactness of the blood vessel 1 during the liquid injection process and preventing the phenomenon of liquid leakage.
[0037] Optionally, the material used for the support tube 311 in this application is an elastic wire material with plasticity under pressure.
[0038] Refer to Figure 3 , on the inner walls of the first connecting piece 32 and the second connecting piece 33, three sleeves 6 corresponding to the three support tubes 311 are fixedly arranged along the circumferential direction. Both ends of the support tube 311 are inserted into the inside of the sleeve 6 and are in contact with the bottom of the sleeve 6. It should be noted here that the reason for designing the sleeve 6 to be installed inside the first connecting piece 32 and the second connecting piece 33 in this application is to prevent the end of the sleeve 6 from scraping the inside of the blood vessel 1 during the movement of the auxiliary device, damaging the cells of the blood vessel 1 and affecting the subsequent collection and culture of cells.
[0039] Refer to Figure 3 , further. In order to prevent the sleeve 6 from separating from the support cylinder during the movement of the auxiliary device in the blood vessel 1, the diameter of the sleeve 6 is designed to be smaller than that between the support tubes 311, that is, the support tubes 311 always form an extrusion on the inner wall of the sleeve 6.
[0040] The material of this auxiliary device can be selected as polypropylene material or a supportive metal material, which can be sterilized at high temperature and reused, or can be made into a disposable sterile package.
[0041] The implementation principle of an auxiliary device for separating umbilical vein endothelial cells in an embodiment of this application is as follows:
[0042] After collecting the umbilical cord, the umbilical vein is peeled from the umbilical cord tissue and cut into multiple segments, and the length thereof shall not exceed the length of the guiding head 2 extending out of the umbilical vein (the umbilical vein can be in a contracted and squeezed state). The lumen of the vein is rinsed with a buffer solution to thoroughly remove the residual blood stains.
[0043] Use the surgical forceps in the left hand to clamp the liquid inlet, use the surgical forceps in the right hand to fix the umbilical vein segment, insert the guiding head 2 into the umbilical vein segment, then use the surgical forceps in the right hand to fix the guiding head 2, and use the surgical forceps in the left hand to grab the endothelial tissue at the edge of the vein opening and pull it in the reverse direction of the guiding head 2 to introduce the filling sac 3 into the umbilical vein segment.
[0044] After the entire device enters the interior of blood vessel 1, use tweezers to grip the ends of the first connecting member 32 and the second connecting member 33 respectively and squeeze them together in the direction towards the middle of blood vessel 1, so that blood vessel 1 can be expanded.
[0045] Finally, the digestive fluid is introduced through the port of syringe 5 and the liquid inlet of the second connecting member 33. During actual use, it is only necessary to control the syringe 5 to inject the digestive fluid on time and in a fixed quantity.
[0046] The above are all preferred embodiments of this application, and the protection scope of this application is not limited thereby. Therefore, all equivalent changes made according to the structure, shape, and principle of this application should be covered within the protection scope of this application.
Claims
1. An auxiliary device for separating umbilical vein endothelial cells, which is disposed inside the blood vessel (1) of the umbilical vein and includes a guiding head (2) in the shape of a syringe needle and a filling sac (3) connected to one end of the guiding head (2) and hollow inside, and is characterized in that: The filling bladder (3) includes a plurality of support tubes (311) distributed circumferentially, and a first connecting member (32) and a second connecting member (33) symmetrically arranged at both ends of the plurality of support tubes (311). The end of the first connecting member (32) away from the support tube (311) is fixedly connected to the guiding head (2). A liquid injection hole (331) is formed through the end of the second connecting member (33) away from the support tube (311). An installation ring (4) is arranged at the position of the liquid injection hole (331) at the end of the second connecting member (33) away from the support tube (311). The inner diameter of the installation ring (4) is equal to the diameter of the liquid injection hole (331). The needle head (51) of a syringe (5) is sleeved on the installation ring (4).
2. The auxiliary device for separating umbilical vein endothelial cells according to claim 1, wherein: Both the first connecting member (32) and the second connecting member (33) are umbrella-shaped structures with the same shape and size. The guiding head (2) is fixedly connected to the tip position of the first connecting member (32). The liquid injection hole (331) is formed at the tip position of the second connecting member (33).
3. An auxiliary device for separating umbilical vein endothelial cells according to claim 2, characterized in that: There are at least three support tubes (311), and the plurality of support tubes (311) are evenly distributed circumferentially along the axis of the guiding head (2).
4. An auxiliary device for separating umbilical vein endothelial cells according to claim 3, characterized in that: The support tube (311) is an arc-shaped structure with bending elasticity and radial elasticity. The plurality of support tubes (311) together enclose an ellipsoid structure (31), and the ellipsoid structure (31) is coaxial with the guiding head (2).
5. An auxiliary device for separating umbilical vein endothelial cells according to claim 4, characterized in that: The diameter of the middle part of the ellipsoid structure (31) in the direction perpendicular to the axis of the guiding head (2) is larger than the inner diameter of the blood vessel (1).
6. The auxiliary device for separating umbilical vein endothelial cells according to claim 5, characterized in that: On the inner sides of the first connecting member (32) and the second connecting member (33), sleeves (6) are integrally arranged circumferentially. Both ends of the support tube (311) are inserted into the inside of the sleeves (6).
7. An auxiliary device for separating umbilical vein endothelial cells according to claim 6, characterized in that: The inner diameter of the sleeve (6) is smaller than the outer diameter of the support tube (311).
8. An auxiliary device for separating umbilical vein endothelial cells according to claim 1, characterized in that: The needle head (51) and the installation ring (4) are in a transition fit of the basic shaft system.