Device for DNA extraction and DNA methylation conversion and purification through paramagnetic particle method

Abstract

The utility model relates to a DNA (deoxyribonucleic acid) extraction and DNA methylation conversion and purification device adopting a paramagnetic particle method. Comprising a machine body, an extraction box body which is fixedly arranged on the machine body and is internally provided with at least one extraction bin group, a conversion box body which is fixedly arranged on the machine body and is internally provided with at least one conversion bin group, a magnetic attraction assembly which adsorbs DNA through magnetic beads, and a driving assembly which is fixedly arranged on the machine body and is connected with the magnetic attraction assembly, the plurality of temperature assemblies are connected with the extraction box body and the conversion box body in a one-to-one correspondence manner, and the extraction bin group comprises a cracking bin chamber, a washing bin chamber, a magnetic bead bin chamber and an elution bin chamber which are sequentially arranged in the extraction box body; the transformation bin group comprises a transformation bin chamber, a desulfurization bin chamber, a washing bin chamber, a magnetic bead bin chamber and an elution bin chamber which are sequentially arranged in the transformation box body, the integrated operation of DNA extraction, DNA methylation transformation and purification can be automatically completed, purified DNA can be conveniently obtained, and instability and pollution in the manual operation process are reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of DNA methylation conversion, and particularly relates to a device for DNA extraction, DNA methylation conversion and purification by a magnetic bead method. Background Technique

[0002] DNA methylation is a process of adding methyl groups to certain bases without changing the base type and DNA sequence. This process mostly occurs in CpG dinucleotide sequences, and CpG-rich regions are mostly located in promoters. Methylation in promoters generally inhibits transcription. Abnormal methylation levels are generally related to many diseases, especially closely related to human development and tumor diseases. Changes in DNA methylation modification occur in the early stage of cancer. By detecting the methylation level at specific sites, cancer can be identified at an early stage. As one of the cancer markers, DNA methylation plays a crucial role in the early screening of tumors.

[0003] Currently, DNA methylation is detected through DNA extraction, conversion, purification, and fluorescence PCR. In the process of conversion and purification, sodium bisulfite and a DNA protection solution are added in an alkaline environment, fully denatured at 95°C, reacted at 64°C for more than 60 minutes, and the product is then purified by nucleic acid column, and finally DNA is obtained for fluorescence PCR analysis.

[0004] Sulfite treatment converts unmethylated cytosine bases into uracil bases, while methylated cytosine bases do not react with bisulfite and can remain in the cytosine base state during bisulfite treatment. Therefore, as long as cytosine bases are detected, it means that the cytosine bases at this position are methylated modified cytosine bases. Then, the methylation sites are identified by fluorescence PCR to facilitate the analysis of DNA methylation. Currently, in the process of DNA extraction, methylation conversion, and purification, most are manually operated, which has the disadvantages of instability, easy contamination, and long test time. Content of the Utility Model

[0005] The purpose of the utility model is to overcome the defects of instability and easy contamination caused by manual operation in the prior art, and provide a device for DNA extraction, DNA methylation conversion and purification by a magnetic bead method, which can automatically complete the integrated operation of DNA extraction, DNA methylation conversion, and purification, facilitate the obtaining of purified DNA, reduce the instability and contamination in the manual operation process, and save the test time.

[0006] The present utility model is implemented as follows. A magnetic bead method DNA extraction and DNA methylation conversion and purification device includes: a machine body, an extraction box body fixedly arranged on the machine body and having at least one extraction chamber group inside, a conversion box body fixedly arranged on the machine body and having at least one conversion chamber group inside, a magnetic adsorption component for adsorbing DNA by magnetic beads, a driving component fixedly arranged inside the machine body and connected to the magnetic adsorption component, and a plurality of temperature components respectively connected to the extraction box body and the conversion box body one by one. The extraction chamber group includes a lysis chamber, a washing chamber, a magnetic bead chamber, and an elution chamber arranged in sequence in the extraction box body. The conversion chamber group includes a conversion chamber, a desulfurization chamber, a washing chamber, a magnetic bead chamber, and an elution chamber arranged in sequence in the conversion box body. The driving component is used to drive the magnetic adsorption component to move and penetrate into or out of the target chambers of the extraction chamber group and the conversion chamber group for liquid oscillation and magnetic bead adsorption and transfer, so as to realize operations such as DNA collection, washing, conversion, and elution.

[0007] In the magnetic bead method DNA extraction and DNA methylation conversion and purification device of the present utility model, the magnetic adsorption component can move under the drive of the driving component and penetrate into the magnetic bead chamber to adsorb magnetic beads, and then enter the lysis chamber to magnetically adsorb the lysed DNA, and use the magnetic beads to drive the DNA to enter the washing chamber and the elution chamber in sequence for washing and elution, thereby realizing the automatic extraction of DNA; the magnetic adsorption component can absorb magnetic beads under the drive of the driving component and then penetrate into the conversion chamber to magnetically adsorb the DNA sample, and transfer it to the washing chamber, the desulfurization chamber, the washing chamber, and the elution chamber in sequence to purify the DNA sample, thereby realizing the automatic conversion and purification of DNA; the magnetic adsorption component can move under the drive of the driving component and penetrate into the magnetic bead chamber to adsorb magnetic beads, and then enter the lysis chamber to magnetically adsorb the lysed DNA, and use the magnetic beads to drive the DNA to enter the washing chamber, the conversion chamber, the washing chamber, the desulfurization chamber, the washing chamber, and the elution chamber in sequence to automatically complete the extraction, conversion, and purification of the DNA sample. The magnetic adsorption component can oscillate the internal liquid after penetrating into the target chamber, and the temperature component can provide a suitable experimental temperature for the extraction and conversion and purification process. The present utility model can automatically complete the integrated operations of DNA extraction, DNA methylation conversion, and purification in cooperation with corresponding reagents, facilitating the obtaining of purified DNA, reducing the instability and contamination in the manual operation process, and saving the test time.

[0008] Preferably, the number of washing chambers in the extraction chamber group is three, the number of desulfurization chambers in the conversion chamber group is one, the number of washing chambers is two, and each chamber in the extraction chamber group and the conversion chamber group is internally divided into a plurality of compartments.

[0009] Preferably, the magnetic attraction assembly includes: a fixing plate connected to the driving assembly, a magnetic rod holder slidably connected to one side of the fixing plate, at least one row of magnetic rods fixed to the bottom of the magnetic rod holder, a magnetic sleeve holder fixed to the lower end of the fixing plate and having an opening therethrough, at least one row of magnetic sleeves fixed to the bottom of the magnetic sleeve holder and opposite to the opening, and a magnetic rod lifting and lowering unit connected to the magnetic rod holder and used to drive the magnetic rods to penetrate into the magnetic sleeves. The driving assembly is used to drive the fixing plate to move so as to integrally penetrate or withdraw the magnetic sleeves with the inserted magnetic rods into or out of the partition cavity of the target chamber.

[0010] Preferably, the magnetic rod lifting and lowering unit includes: a moving block slidably connected to one side of the fixing plate, a magnetic rod guide fixed to the side of the moving block away from the fixing plate and fixedly connected to the magnetic rod holder, a magnetic rod lifting motor fixed to one side of the top of the fixing plate, and a magnetic rod screw rod connected to the output end of the magnetic rod lifting motor and threadedly connected to the moving block. The magnetic sleeve holder is connected with a magnetic sleeve guide fixedly installed at the lower end of the fixing plate.

[0011] Preferably, the extraction box body and the conversion box body are arranged alternately at intervals and the number of each is two. Two extraction chamber groups are sequentially arranged inside the extraction box body, and two conversion chamber groups are sequentially arranged inside the conversion box body. The magnetic sleeve holder is symmetrically fixed to both ends of the magnetic sleeve guide and has two rows of magnetic sleeves at the bottom. The magnetic rod holder is symmetrically fixed to both ends of the magnetic rod guide and has two rows of magnetic rods at the bottom corresponding to the two rows of magnetic sleeves one by one.

[0012] Preferably, the driving assembly includes: an X-axis driving unit fixed to the machine body and a Z-axis oscillation unit connected to the X-axis driving unit. The fixing plate is connected to the Z-axis oscillation unit. The device further includes a drip prevention assembly connected to the X-axis driving unit and used to be placed below the magnetic attraction assembly during its movement to prevent liquid from dripping into the extraction chamber group or the conversion chamber group.

[0013] Preferably, the X-axis driving unit includes: a support frame fixed to the machine body, an X-direction guide rail fixedly installed on the support frame and extending in the horizontal direction, an X-axis driving motor fixed to the side of the support frame, an X-direction screw rod connected to the output end of the X-axis driving motor, and a moving frame body threadedly connected to the X-direction screw rod and slidably connected to the X-direction guide rail.

[0014] Preferably, the Z-axis oscillation unit includes: a Z-axis oscillation motor fixed to the top end of the moving frame body, a Z-direction screw rod connected to the output end of the Z-axis oscillation motor, a Z-direction guide rail fixed to the side of the moving frame body close to the magnetic attraction assembly, a connecting block with one end threadedly connected to the Z-direction screw rod and the other end fixedly connected to the fixing plate, and the fixing plate is slidably connected to the Z-direction guide rail.

[0015] Preferably, the anti-drip assembly includes: a moving table fixedly installed at the bottom of the moving frame body and disposed close to one side of the magnetic attraction assembly, a slide rail fixedly arranged above the moving table and extending along the arrangement direction of the extraction box body and the conversion box body, an anti-drip plate slidably installed on the slide rail and used to be placed under the magnetic sleeve and move synchronously therewith, and a pulley set connected to the anti-drip plate and used to drive the anti-drip plate to move along the slide rail.

[0016] Preferably, the temperature assembly includes: a heat sink fixedly installed on the machine body, a temperature control device fixedly installed on the top of the heat sink, an incubation tank arranged on the top of the temperature control device, and a cover plate covering the top of the incubation tank and used to support the extraction box body or the conversion box body.

[0017] Compared with the prior art, the beneficial effects of the present utility model are as follows: The magnetic attraction assembly can move under the drive of the drive assembly, penetrate into the magnetic bead chamber to adsorb magnetic beads, then enter the lysis chamber to magnetically attract the lysed DNA, and drive the DNA to enter the washing chamber and the elution chamber in sequence for washing and elution, thereby realizing the automatic extraction of DNA; The magnetic attraction assembly can suck magnetic beads under the drive of the drive assembly, penetrate into the conversion chamber, magnetically attract the DNA sample, and transfer it to the washing chamber, the desulfurization chamber, the washing chamber and the elution chamber in sequence to purify the DNA sample, thereby realizing the automatic conversion and purification of DNA; The magnetic attraction assembly can move under the drive of the drive assembly, penetrate into the magnetic bead chamber to adsorb magnetic beads, then enter the lysis chamber to magnetically attract the lysed DNA, and drive the DNA to enter the washing chamber, the conversion chamber, the washing chamber, the desulfurization chamber, the washing chamber and the elution chamber in sequence to automatically complete the extraction, conversion and purification of the DNA sample. The magnetic attraction assembly can oscillate the internal liquid after penetrating into the target chamber, and the temperature assembly can provide a suitable experimental temperature for the extraction and conversion and purification processes. The present utility model can automatically complete the integrated operations of DNA extraction, DNA methylation conversion and purification in cooperation with corresponding reagents, facilitating the obtaining of purified DNA, reducing the instability and contamination in the manual operation process, and saving the test time. Description of the Drawings

[0018] Figure 1 is a three-dimensional structure diagram of a magnetic bead method DNA extraction and DNA methylation conversion and purification device provided by the present utility model;

[0019] Figure 2 is a three-dimensional structure diagram of an extraction box body of a magnetic bead method DNA extraction and DNA methylation conversion and purification device provided by the present utility model;

[0020] Figure 3 is a three-dimensional structure diagram of a conversion box body of a magnetic bead method DNA extraction and DNA methylation conversion and purification device provided by the present utility model;

[0021] Figure 4 This is a three-dimensional structure diagram of another perspective of a magnetic bead method DNA extraction and DNA methylation conversion and purification device provided by the present utility model;

[0022] Figure 5 This is a three-dimensional structure diagram of the magnetic attraction component and the drive component of a magnetic bead method DNA extraction and DNA methylation conversion and purification device provided by the present utility model;

[0023] Figure 6 This is a three-dimensional structure diagram of the anti-drip component of a magnetic bead method DNA extraction and DNA methylation conversion and purification device provided by the present utility model;

[0024] Figure 7 This is an exploded view of the temperature component of a magnetic bead method DNA extraction and DNA methylation conversion and purification device provided by the present utility model.

[0025] In the drawings: 1 - body, 2 - extraction box body, 21 - extraction chamber group, 3 - conversion box body, 31 - conversion chamber group, 4 - magnetic attraction component, 41 - fixing plate, 42 - magnetic rod, 43 - magnetic sleeve, 44 - magnetic rod holder, 45 - magnetic sleeve holder, 46 - magnetic rod lifting and lowering unit, 461 - moving block, 462 - magnetic rod guide frame, 463 - magnetic rod lifting and lowering motor, 464 - magnetic rod lead screw, 465 - magnetic sleeve guide frame, 5 - drive component, 51 - X-axis drive unit, 511 - support frame, 512 - X-direction guide rail, 513 - X-axis drive motor, 514 - X-direction lead screw, 515 - moving frame body, 52 - Z-axis oscillation unit, 521 - Z-axis oscillation motor, 522 - Z-direction lead screw, 523 - Z-direction guide rail, 524 - connecting block, 53 - anti-drip component, 531 - moving table, 532 - slide rail, 533 - anti-drip plate, 534 - pulley group, 6 - temperature component, 61 - heat sink, 62 - temperature control device, 63 - incubation tank, 64 - cover plate, 7 - filtration module, 8 - lighting lamp, 9 - ultraviolet lamp, 10 - operation screen. Specific embodiments

[0026] To make the objectives, technical solutions, and advantages of the embodiments of the present application clearer, the following will further describe the specific technical solutions of the present application in detail with reference to the accompanying drawings in the embodiments of the present application. The following embodiments are used to illustrate the present application but are not used to limit the scope of the present application.

[0027] In the embodiments of the present application, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the embodiments of the present application, unless otherwise stated, the meaning of "a plurality" is two or more.

[0028] In addition, in the embodiments of the present application, orientation terms such as "upper", "lower", "left", and "right" are defined relative to the orientation of the components shown in the drawings. It should be understood that these directional terms are relative concepts, which are used for relative description and clarification, and they can change accordingly with the change of the orientation of the components placed in the drawings.

[0029] In the embodiments of the present application, unless otherwise clearly specified and limited, the term "connection" should be understood in a broad sense. For example, "connection" can be a fixed connection, a detachable connection, or integrated; it can be directly connected or indirectly connected through an intermediate medium.

[0030] In the embodiments of the present application, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "comprising a..." does not exclude the existence of additional identical elements in the process, method, article or device comprising the element.

[0031] In the embodiments of the present application, words such as "exemplary" or "for example" are used to represent examples, illustrations or explanations. Any embodiment or design solution described as "exemplary" or "for example" in the embodiments of the present application should not be construed as more preferred or more advantageous than other embodiments or design solutions. Rather, the use of words such as "exemplary" or "for example" is intended to present relevant concepts in a specific manner.

[0032] Embodiment 1

[0033] As Figures 1 - 5As shown in the figure, it is a structural diagram of a device for DNA extraction and DNA methylation conversion and purification provided by the present utility model, including: a body 1, an extraction box body 2 fixedly arranged on the body 1 and having at least one extraction chamber group 21 inside, a conversion box body 3 fixedly arranged on the body 1 and having at least one conversion chamber group 31 inside, a magnetic bead adsorption component 4 for adsorbing DNA by magnetic beads, a driving component 5 fixedly arranged inside the body 1 and connected to the magnetic bead adsorption component 4, and a plurality of temperature components 6 connected to the extraction box body 2 and the conversion box body 3 in one-to-one correspondence. The extraction chamber group 21 includes a lysis chamber, a washing chamber, a magnetic bead chamber, and an elution chamber arranged in sequence inside the extraction box body 2. The conversion chamber group 31 includes a conversion chamber, a desulfurization chamber, a washing chamber, a magnetic bead chamber, and an elution chamber arranged in sequence inside the conversion box body 3. The driving component 5 is used to drive the magnetic bead adsorption component 4 to move and penetrate into or out of the target chambers of the extraction chamber group 21 and the conversion chamber group 31 to perform liquid oscillation and magnetic bead adsorption and transfer, so as to realize operations such as DNA collection, washing, conversion, and elution.

[0034] In actual application of this embodiment, the magnetic bead adsorption component 4 can move under the drive of the driving component 5 and penetrate into the magnetic bead chamber to adsorb magnetic beads, and then enter the lysis chamber to magnetically adsorb the lysed DNA, and use the magnetic beads to drive the DNA to enter the washing chamber and the elution chamber in sequence for washing and elution, thereby realizing the automatic extraction of DNA; the magnetic bead adsorption component 4 can absorb magnetic beads under the drive of the driving component 5 and then penetrate into the conversion chamber to magnetically adsorb the DNA sample, and transfer it to the washing chamber, desulfurization chamber, washing chamber, and elution chamber in sequence to purify the DNA sample, thereby realizing the automatic conversion and purification of DNA; the magnetic bead adsorption component can move under the drive of the driving component and penetrate into the magnetic bead chamber to adsorb magnetic beads, and then enter the lysis chamber to magnetically adsorb the lysed DNA, and use the magnetic beads to drive the DNA to enter the washing chamber, conversion chamber, washing chamber, desulfurization chamber, washing chamber, and elution chamber in sequence to automatically complete the extraction, conversion, and purification of the DNA sample. After the magnetic bead adsorption component 4 penetrates into the target chamber, it can oscillate the liquid inside it. The temperature component 6 can provide a suitable experimental temperature for the extraction and conversion and purification process, and thus can complete the integrated operation of DNA extraction, DNA methylation conversion, and purification, facilitating the obtaining of purified DNA, reducing the instability and contamination in the manual operation process, and saving the test time.

[0035] It should be noted that a lysis solution, a washing solution, a magnetic bead solution, and an elution solution are sequentially provided inside the lysis chamber, the washing chamber, the magnetic bead chamber, and the elution chamber. Specific solutions can be selected according to needs. The magnetic bead solution contains magnetic beads. When performing DNA extraction, first manually add the cell sample to be extracted into the lysis chamber. Subsequently, the magnetic attraction assembly 4 first penetrates into the magnetic bead chamber to adsorb the magnetic beads inside, and then sequentially penetrates into the lysis chamber, the washing chamber, and the elution chamber to perform lysis, washing, and elution on the cell sample to be extracted in sequence, and obtain the extracted DNA in the elution chamber of the extraction box body 2.

[0036] It should be noted that a transformation reagent, a desulfurization solution, a washing solution, a magnetic bead solution, and an elution solution are sequentially provided inside the transformation chamber, the desulfurization chamber, the washing chamber, the magnetic bead chamber, and the elution chamber. Specific solutions can be selected according to needs. When performing DNA transformation and purification, the DNA sample to be transformed and purified can be manually added into the transformation chamber first, or the magnetic beads adsorbed with DNA inside the washing chamber of the extraction box body 2 can be transferred into the transformation chamber by using the magnetic attraction assembly 4. Subsequently, the magnetic attraction assembly 4 sequentially penetrates into the washing chamber, the desulfurization chamber, the washing chamber, and the elution chamber to perform transformation, desulfurization, washing, and elution on the DNA sample in sequence, and further obtain the transformed and purified DNA in the elution chamber of the transformation box body 3.

[0037] In one case of this embodiment, as Figure 2 and Figure 3 shown, the number of washing chambers in the extraction chamber group 21 is three, the number of desulfurization chambers in the transformation chamber group 31 is one, the number of washing chambers is two, and each chamber inside the extraction chamber group 21 and the transformation chamber group 31 is divided into multiple compartments.

[0038] It can be known that by arranging three washing chambers inside the extraction chamber group 21, the DNA that has undergone lysis can be washed inside the three washing chambers in sequence, so that the washed DNA is adsorbed on the magnetic attraction assembly 4. Each chamber inside the extraction chamber group 21 and the transformation chamber group 31 is divided into multiple compartments, and multiple compartments can be used to extract multiple DNAs simultaneously.

[0039] Furthermore, the magnetic attraction assembly 4 includes: a fixing plate 41 connected to the driving assembly 5, a magnetic rod holder 44 slidably connected to one side of the fixing plate 41, at least one row of magnetic rods 42 fixed to the bottom of the magnetic rod holder 44, a magnetic sleeve holder 45 fixedly connected to the lower end of the fixing plate 41 and having an opening penetrating therethrough, at least one row of magnetic sleeves 43 fixed to the bottom of the magnetic sleeve holder 45 and opposite to the opening, and a magnetic rod lifting and lowering unit 46 connected to the magnetic rod holder 44 and used to drive the magnetic rods 42 to penetrate into the magnetic sleeves 43. The driving assembly 5 is used to drive the fixing plate 41 to move so as to penetrate the entire magnetic sleeve 43 with the magnetic rods 42 penetrated therein into or out of the compartment of the target chamber.

[0040] It can be known that by using the fixing plate 41 connected to the driving assembly 5, the driving assembly 5 can drive the fixing plate 41 to move along the horizontal direction and the vertical direction respectively; by using the magnetic rod holder 44 and at least one column of magnetic rods 42 fixed to the bottom thereof, a group of magnetic rods 42 can be first inserted into the inside of the magnetic sleeve 43 by the magnetic rod lifting and lowering unit 46, and then the driving assembly 5 connected to the fixing plate 41 can be used to drive it to move, and insert or extract it into the partition cavity of the target chamber, so as to stir the target chamber or transfer DNA.

[0041] It should be noted that by using the magnetism of the magnetic rod 42, the magnetic beads inside the magnetic bead chamber can be attracted by the magnetic rod 42 and attached to the outer surface of the magnetic sleeve 43, and the two are inserted into the target chamber as a whole; and each chamber in the extraction chamber group 21 and the conversion chamber group 31 is divided into multiple partition cavities, so it can correspond one by one to one column of magnetic rods 42 and magnetic sleeves 43, so as to transfer or stir the DNA inside multiple partition cavities at the same time.

[0042] Furthermore, the magnetic rod lifting and lowering unit 46 includes: a moving block 461 slidably connected to one side of the fixing plate 41, a magnetic rod guide frame 462 fixed to the side of the moving block 461 away from the fixing plate 41 and fixedly connected to the magnetic rod holder 44, a magnetic rod lifting and lowering motor 463 fixed to one side of the top of the fixing plate 41, and a magnetic rod lead screw 464 connected to the output end of the magnetic rod lifting and lowering motor 463 and threadedly connected to the moving block 461. The magnetic sleeve holder 45 is connected to a magnetic sleeve guide frame 465 fixedly installed at the lower end of the fixing plate 41.

[0043] It can be known that by using the magnetic rod lifting and lowering motor 463 to drive the magnetic rod lead screw 464 to rotate, the moving block 461 slidably connected to one side of the fixing plate 41 can be driven to move along the fixing plate 41, and then the magnetic rod guide frame 462 can be driven to move up and down, driving the magnetic rod 42 to penetrate into or out of the magnetic sleeve 43.

[0044] It should be noted that in this embodiment, in addition to using the screw-nut structure for the magnetic rod lifting and lowering unit 46, a cylinder or a hydraulic rod can also be used to realize the vertical movement of the magnetic rod 42, and this embodiment does not make specific limitations here.

[0045] In one case of this embodiment, the extraction box body 2 and the conversion box body 3 are arranged alternately at intervals and the number of each is two. Two extraction chamber groups 21 are sequentially arranged inside the extraction box body 2, and two conversion chamber groups 31 are sequentially arranged inside the conversion box body 3. The magnetic sleeve holder 45 is symmetrically fixed at both ends of the magnetic sleeve guide frame 465 and two columns of magnetic sleeves 43 are arranged at the bottom, and the magnetic rod holder 44 is symmetrically fixed at both ends of the magnetic rod guide frame 462 and two columns of magnetic rods 42 corresponding one by one to the two columns of magnetic sleeves 43 are arranged at the bottom.

[0046] It can be known that two extraction bin groups 21 are provided inside the extraction box body 2, and two conversion bin groups 31 are provided inside the conversion box body 3, which can correspond to two columns of magnetic sleeves 43 and two columns of magnetic rods 42. Thus, inside one extraction box body 2 and one conversion box body 3, DNA extraction and conversion purification of two different samples can be carried out simultaneously. By arranging the extraction box body 2 and the conversion box body 3 at intervals into two, different paired extraction box bodies 2 and conversion box bodies 3 can be used to perform different extraction operations respectively to meet different experimental requirements.

[0047] It should be noted that in this embodiment, the extraction box body 2 and the conversion box body 3 are arranged in pairs, and the number can be multiple groups to meet different experimental requirements. Therefore, the number of the extraction box body 2 and the conversion box body 3 in this embodiment is not a restrictive regulation.

[0048] Exemplarily, the magnetic rod lifting and lowering motor 463 can be a stepper motor or a servo motor. The number of a column of magnetic rods 42 or a column of magnetic sleeves 43 fixed below the magnetic rod holder 44 can be equal to the number of partition chambers inside each bin chamber, so as to realize the extraction and conversion of DNA inside the corresponding partition chambers. The number of the magnetic rods 42, the magnetic sleeves 43 and the partition chambers is not a restrictive regulation.

[0049] Embodiment 2

[0050] As Figures 4 - 6 shown, on the basis of Embodiment 1, the driving assembly 5 includes: an X-axis driving unit 51 fixed on the machine body 1 and a Z-axis oscillation unit 52 connected to the X-axis driving unit 51. The fixing plate 41 is connected to the Z-axis oscillation unit 52. The device further includes a drip prevention component 53 connected to the X-axis driving unit 51 and used to be placed below the magnetic attraction assembly 4 during its movement to prevent liquid from dripping into the extraction bin group 21 or the conversion bin group 31.

[0051] In actual application of this embodiment, by using the X-axis driving unit 51 and the Z-axis oscillation unit 52, the fixing plate 41 and the magnetic rod lifting and lowering unit 46 can be driven to move up and down and horizontally. The drip prevention component 53 connected to the X-axis driving unit 51 can be placed below the magnetic attraction assembly 4 during its horizontal movement to prevent liquid from dripping into the partition chambers inside the extraction bin group 21 or the conversion bin group 31, reducing the influence on the experimental results and improving the detection accuracy.

[0052] Furthermore, the X-axis driving unit 51 includes: a support frame 511 fixed on the machine body 1, an X-direction guide rail 512 fixed on the support frame 511 and extending in the horizontal direction, an X-axis driving motor 513 fixed on the side of the support frame 511, an X-direction lead screw 514 connected to the output end of the X-axis driving motor 513, and a moving frame body 515 threadedly connected to the X-direction lead screw 514 and slidably connected to the X-direction guide rail 512.

[0053] Furthermore, the Z-axis oscillation unit 52 includes: a Z-axis oscillation motor 521 fixedly installed at the top end of the moving frame 515, a Z-direction screw rod 522 connected to the output end of the Z-axis oscillation motor 521, a Z-direction guide rail 523 fixedly installed on the moving frame 515 close to one side of the magnetic attraction assembly 4, a connecting block 524 with one end threadedly connected to the Z-direction screw rod 522 and the other end fixedly connected to the fixing plate 41, and the fixing plate 41 is slidably connected to the Z-direction guide rail 523.

[0054] It can be known that the X-axis driving motor 513 drives the X-direction screw rod 514 to rotate, and then drives the moving frame 515 to move along the X-direction guide rail 512 to realize the horizontal movement of the moving frame 515. The Z-axis oscillation motor 521 can drive the Z-direction screw rod 522 to rotate, and then drive the connecting block 524 and the fixing plate 41 to move in the vertical direction to realize the horizontal and vertical movement of the magnetic rod 42 and the magnetic sleeve 43.

[0055] In actual operation of this embodiment, the moving frame 515 includes: a bent plate threadedly connected to the X-direction screw rod 514, a first vertical plate fixedly installed on the top of the bent plate and slidably connected to the X-direction guide rail 512, a horizontal plate fixedly installed on the top of the first vertical plate, and a second vertical plate fixedly installed on one side of the horizontal plate close to the magnetic attraction assembly 4. The Z-axis oscillation motor 521 is fixed on the top of the horizontal plate, the Z-direction guide rail 523 is fixedly installed on the side of the second vertical plate, and the anti-drip assembly 53 is fixedly installed at the bottom end of the second vertical plate.

[0056] It should be noted that setting the moving frame 515 as a bent plate, a first vertical plate and a second vertical plate can fixedly install the Z-axis oscillation motor 521 and can also be connected to the X-direction screw rod 514 to realize the horizontal movement of the entire moving frame 515.

[0057] Exemplarily, for the X-axis driving unit 51 and the Z-axis oscillation unit 52 in this embodiment, in addition to adopting the screw-nut structure, linear driving can also be carried out by means of air cylinders, hydraulic rods, gear racks, etc. This embodiment does not make specific limitations here.

[0058] Embodiment 3

[0059] As Figure 6 and Figure 7 shown, on the basis of Embodiment 1 and Embodiment 2, the anti-drip assembly 53 includes: a moving table 531 fixedly installed at the bottom of the moving frame 515 and arranged close to one side of the magnetic attraction assembly 4, a slide rail 532 fixedly installed above the moving table 531 and extending along the arrangement direction of the extraction box body 2 and the conversion box body 3, a drip-proof plate 533 slidably installed on the slide rail 532 and used to be placed under the magnetic sleeve 43 and move synchronously with it, and a pulley group 534 connected to the drip-proof plate 533 and used to drive the drip-proof plate 533 to move along the slide rail 532.

[0060] Specifically, the mobile station 531 can move horizontally along with the mobile frame 515. The slide rail 532 fixed above the mobile station 531 can provide a guiding function for the movement of the drip-proof plate 533. Driven by the pulley group 534, the drip-proof plate 533 can move along the slide rail 532, so as to be placed below the magnetic sleeve 43 and move synchronously with it, preventing the impact caused by liquid dripping on the extraction box body 2 and the conversion box body 3.

[0061] In actual operation of this embodiment, as Figure 6 shown, the pulley group 534 includes: a stepper motor fixedly installed at one end of the mobile station 531, an idler wheel rotatably installed at the other end of the mobile station 531, a synchronous wheel connected to the output end of the rotating motor, and a gear synchronous belt wound between the synchronous wheel and the idler wheel and fixedly connected to the drip-proof plate 533.

[0062] Furthermore, as Figure 7 shown, the temperature component 6 includes: a heat sink 61 fixedly installed on the machine body 1, a temperature control device 62 fixedly installed on the top of the heat sink 61, an incubation tank 63 arranged on the top of the temperature control device 62, and a cover plate 64 covering the top of the incubation tank 63 and used to support the extraction box body 2 or the conversion box body 3.

[0063] It can be known that the temperature control device 62 can be used to adjust the temperature of the incubation tank 63 and the cover plate 64 in real time, so as to conveniently adjust the temperature of the corresponding chambers of the extraction box body 2 and the conversion box body 3; while the heat sink 61 arranged below the temperature control device 62 can dissipate heat from the temperature control device 62 in time to ensure the smooth progress of temperature adjustment.

[0064] Further, the temperature control device 62 is equipped with a temperature sensor and an over-temperature protector, and a plurality of slots corresponding to the incubation tank 63 are arranged inside the cover plate 64. By using the slots and the incubation tank 63, one or several chambers inside the extraction box body 2 and the conversion box body 3 can be heated or cooled as needed to ensure the smooth implementation of the DNA extraction and conversion processes. The positions of the incubation tank 63 and the slots can be selected as needed, and this embodiment does not make specific limitations here.

[0065] In actual operation of this embodiment, the temperature component 6 can be arranged in one-to-one correspondence with the extraction box body 2 and the conversion box body 3. The extraction box body 2 and the conversion box body 3 are placed on the top of the temperature component 6, and the temperature can be controlled as needed to meet the temperature requirements of different extractions or conversions.

[0066] Exemplarily, the temperature control device 62 can be a TEC temperature control electrical device, and of course it can also be other temperature control components. This embodiment does not make specific limitations here.

[0067] In actual application of this embodiment, asFigure 1 As shown, the device further includes: a filtration module 7, a lighting lamp 8, an ultraviolet lamp 9, and an operation screen 10 fixedly installed on the top of the body 1. Exemplarily, the filtration module 7 can be a filtration fan or a fan filtration unit.

[0068] Specifically, the filtration module 7 can be used to filter the air inside the body 1 to reduce the impact on the extraction, transformation, and purification process. The ultraviolet lamp 9 can sterilize and disinfect the surrounding area, and the operation screen 10 can control each part of the device in real time, making the operation more convenient and reliable.

[0069] In the above embodiment of the present utility model, a device for DNA extraction and DNA methylation transformation and purification by the magnetic bead method is provided. The magnetic adsorption component 4 can move under the drive of the drive component 5 and penetrate into the magnetic bead chamber to adsorb magnetic beads, and then enter the lysis chamber to magnetically adsorb the lysed DNA. Then, the magnetic beads are used to drive the DNA to enter the washing chamber and the elution chamber in sequence for washing and elution, thereby realizing the automatic extraction of DNA. The magnetic adsorption component 4 can absorb magnetic beads under the drive of the drive component 5 and then penetrate into the transformation chamber to magnetically adsorb the DNA sample, and transfer it to the washing chamber, the desulfurization chamber, the washing chamber, and the elution chamber in sequence to purify the DNA sample, thereby realizing the automatic transformation and purification of DNA. The magnetic adsorption component can move under the drive of the drive component and penetrate into the magnetic bead chamber to adsorb magnetic beads, and then enter the lysis chamber to magnetically adsorb the lysed DNA. Then, the magnetic beads are used to drive the DNA to enter the washing chamber, the transformation chamber, the washing chamber, the desulfurization chamber, the washing chamber, and the elution chamber in sequence to automatically complete the extraction, transformation, and purification of the DNA sample. The magnetic adsorption component 4 can shake the internal liquid after penetrating into the target chamber. The temperature component 6 can provide a suitable experimental temperature for the extraction and transformation and purification process, and then can complete the integrated operation of DNA extraction, DNA methylation transformation, and purification, facilitating the obtaining of purified DNA, reducing the instability and contamination in the manual operation process, and saving the test time.

[0070] The above are only the preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present utility model shall be included in the protection scope of the present utility model.

Claims

1. A magnetic bead method DNA extraction and DNA methylation conversion and purification device, characterized in that, Including: a body (1), an extraction box body (2) fixedly arranged on the body (1) and having at least one extraction chamber group (21) inside, a conversion box body (3) fixedly arranged on the body (1) and having at least one conversion chamber group (31) inside, a magnetic adsorption component (4) for adsorbing DNA by magnetic beads, a driving component (5) fixedly arranged inside the body (1) and connected to the magnetic adsorption component (4), and a plurality of temperature components (6) respectively connected to the extraction box body (2) and the conversion box body (3) in one-to-one correspondence. The extraction chamber group (21) includes a lysis chamber, a washing chamber, a magnetic bead chamber, and an elution chamber arranged in sequence in the extraction box body (2). The conversion chamber group (31) includes a conversion chamber, a desulfurization chamber, a washing chamber, a magnetic bead chamber, and an elution chamber arranged in sequence in the conversion box body (3). The driving component (5) is used to drive the magnetic adsorption component (4) to move and penetrate into or out of the target chambers of the extraction chamber group (21) and the conversion chamber group (31) for DNA transfer and liquid oscillation.

2. The magnetic bead method DNA extraction and DNA methylation conversion and purification device according to claim 1, wherein The number of washing chambers in the extraction chamber group (21) is three, the number of desulfurization chambers in the conversion chamber group (31) is one, the number of washing chambers is two, and each chamber inside the extraction chamber group (21) and the conversion chamber group (31) is divided into a plurality of partition cavities.

3. The magnetic bead method DNA extraction and DNA methylation conversion and purification device according to claim 2, wherein, The magnetic adsorption component (4) includes: a fixing plate (41) connected to the driving component (5), a magnetic rod holder (44) slidably connected to one side of the fixing plate (41), at least one row of magnetic rods (42) fixedly arranged at the bottom of the magnetic rod holder (44), a magnetic sleeve holder (45) fixedly connected to the lower end of the fixing plate (41) and having an opening penetrating inside, at least one row of magnetic sleeves (43) fixedly arranged at the bottom of the magnetic sleeve holder (45) and opposite to the opening, and a magnetic rod lifting and lowering unit (46) connected to the magnetic rod holder (44) and used to drive the magnetic rods (42) to penetrate into the magnetic sleeves (43). The driving component (5) is used to drive the fixing plate (41) to move so as to integrally penetrate into or out of the partition cavity of the target chamber with the magnetic sleeves (43) after the magnetic rods (42) penetrate.

4. A magnetic bead-based DNA extraction and DNA methylation conversion and purification device according to claim 3, characterized in that, The magnetic rod lifting and lowering unit (46) includes: a moving block (461) slidably connected to one side of the fixing plate (41), a magnetic rod guide frame (462) fixedly arranged on the side of the moving block (461) away from the fixing plate (41) and fixedly connected to the magnetic rod holder (44), a magnetic rod lifting and lowering motor (463) fixedly arranged on one side of the top of the fixing plate (41), and a magnetic rod lead screw (464) connected to the output end of the magnetic rod lifting and lowering motor (463) and threadedly connected to the moving block (461). The magnetic sleeve holder (45) is connected to a magnetic sleeve guide frame (465) fixedly installed at the lower end of the fixing plate (41).

5. A magnetic bead-based DNA extraction and DNA methylation conversion and purification device according to claim 4, characterized in that, The extraction box body (2) and the conversion box body (3) are arranged alternately and at intervals, and the number of each is two. Two extraction bin groups (21) are successively arranged inside the extraction box body (2), and two conversion bin groups (31) are successively arranged inside the conversion box body (3). The magnetic sleeve holders (45) are symmetrically fixed at both ends of the magnetic sleeve guide (465), and two rows of magnetic sleeves (43) are arranged at the bottom. The magnetic bar holders (44) are symmetrically fixed at both ends of the magnetic bar guide (462), and two rows of magnetic bars (42) corresponding to the two rows of magnetic sleeves (43) one by one are arranged at the bottom.

6. A magnetic bead-based DNA extraction and DNA methylation conversion and purification device according to any one of claims 3-5, characterized in that, The driving assembly (5) includes: an X-axis driving unit (51) fixed on the machine body (1) and a Z-axis oscillation unit (52) connected to the X-axis driving unit (51). The fixing plate (41) is connected to the Z-axis oscillation unit (52). The device further includes a drip prevention assembly (53) connected to the X-axis driving unit (51) and used to be placed under the magnetic attraction assembly (4) during the movement of the magnetic attraction assembly (4) to prevent liquid from dripping into the extraction bin group (21) or the conversion bin group (31).

7. A magnetic bead method DNA extraction and DNA methylation conversion and purification device according to claim 6, characterized in that, The X-axis driving unit (51) includes: a support frame (511) fixed on the machine body (1), an X-direction guide rail (512) fixedly installed on the support frame (511) and extending in the horizontal direction, an X-axis driving motor (513) fixed on the side of the support frame (511), an X-direction lead screw (514) connected to the output end of the X-axis driving motor (513), and a moving frame body (515) threadedly connected to the X-direction lead screw (514) and slidably connected to the X-direction guide rail (512).

8. A magnetic bead-based DNA extraction and DNA methylation conversion and purification device according to claim 7, characterized in that, The Z-axis oscillation unit (52) includes: a Z-axis oscillation motor (521) fixed on the top end of the moving frame body (515), a Z-direction lead screw (522) connected to the output end of the Z-axis oscillation motor (521), a Z-direction guide rail (523) fixed on the side of the moving frame body (515) close to the magnetic attraction assembly (4), a connecting block (524) with one end threadedly connected to the Z-direction lead screw (522) and the other end fixedly connected to the fixing plate (41), and the fixing plate (41) is slidably connected to the Z-direction guide rail (523).

9. A magnetic bead-based DNA extraction and DNA methylation conversion and purification device according to claim 7, wherein, The drip prevention assembly (53) includes: a moving table (531) fixedly installed at the bottom of the moving frame body (515) and arranged on the side close to the magnetic attraction assembly (4), a slide rail (532) fixed above the moving table (531) and extending along the arrangement direction of the extraction box body (2) and the conversion box body (3), a drip prevention plate (533) slidably installed on the slide rail (532) and used to be placed under the magnetic sleeve (43) and move synchronously with it, and a pulley group (534) connected to the drip prevention plate (533) and used to drive the drip prevention plate (533) to move along the slide rail (532).

10. The magnetic bead method DNA extraction and DNA methylation conversion and purification device according to claim 1, wherein The temperature component (6) includes: a heat sink (61) fixedly provided on the body (1), a temperature control device (62) fixedly installed on the top of the heat sink (61), an incubation tank (63) provided on the top of the temperature control device (62), and a cover plate (64) covering the top of the incubation tank (63) and used for supporting the extraction box body (2) or the conversion box body (3).

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