Feeding device of western blot instrument

Abstract

The utility model provides a western blot instrument feeding device. Relates to the technical field of protein analysis auxiliary tools. The western blot instrument feeding device comprises a portal frame, a rotating shaft is rotatably mounted at the top of the portal frame, a connecting disc is fixedly mounted at the top end of the rotating shaft, two supporting arms which are symmetrically arranged are fixedly mounted on the connecting disc, and first telescopic air cylinders are fixedly mounted at the tops of the two supporting arms; first n-shaped frames are fixedly installed on output shafts of the two first telescopic air cylinders correspondingly, second telescopic air cylinders are fixedly installed on the outer walls of the two sides of the two first n-shaped frames correspondingly, and output shafts of the four second telescopic air cylinders penetrate through the corresponding first n-shaped frames correspondingly and are in sliding connection with the corresponding first n-shaped frames correspondingly. According to the utility model, the time can be fully utilized, the storage bottle can be quickly replaced, and the analysis and detection efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein analysis auxiliary tools, in particular to a protein blotting instrument loading device. Background Art

[0002] A protein blotting instrument is an analytical instrument used in biology and basic medicine. It can perform various protein sample separations, immunoassays, qualitative and quantitative analyses, and is widely used in protein property identification, protein quantitative analysis, and other fields. During the use of a protein blotting instrument, a storage bottle containing protein needs to be placed in the instrument before the corresponding detection work is performed.

[0003] For example, patent document CN213780118U proposes a loading device for a protein blot instrument. This device uses a pneumatic cylinder to push a clamping device to load and unload materials, or a manual operation and a clamping device to work together. This reduces the number of tests required, and the manual operation and loading device work together to improve work efficiency.

[0004] However, during operation, it was found that although the storage bottle can be automatically picked up and placed, each time the storage bottle is taken out of the instrument, it is necessary to put the storage bottle back into the clamping device for fixation and then put it into the instrument for analysis and detection. The time utilization rate is not high and the analysis efficiency is still relatively low.

[0005] Therefore, it is necessary to provide a new protein blotting instrument loading device to solve the above technical problems. Utility Model Content

[0006] The utility model aims to provide a protein blotting instrument loading device which can make full use of time, quickly replace storage bottles and improve analysis and detection efficiency.

[0007] To solve the above technical problems, the feeding device of the protein blotting instrument provided by the utility model includes: a gantry, a rotating shaft is rotatably installed at the top of the gantry, a connecting disk is fixedly installed at the top end of the rotating shaft, two symmetrically arranged support arms are fixedly installed on the connecting disk, first telescopic cylinders are fixedly installed at the tops of the two support arms, first U-shaped frames are fixedly installed on the output shafts of the two first telescopic cylinders, second telescopic cylinders are fixedly installed on the outer walls of both sides of the two first U-shaped frames, the output shafts of the four second telescopic cylinders respectively penetrate through the corresponding first U-shaped frames and are slidably connected to the corresponding first U-shaped frames, clamping pieces are fixedly installed on the output shafts of the four second telescopic cylinders, clamping blocks are fixedly installed on the four clamping pieces, and the four clamping blocks are adapted to each other in pairs. A placing table is arranged on one side of the gantry. One section of the placing table far from the support arm is an inclined tabletop, and one section close to the support arm is a horizontal tabletop, and this horizontal tabletop is flush with the top tabletop of the support arm. A folded fixed frame is fixedly installed on the top of the placing table, a second U-shaped frame is fixedly installed on the inner wall of the top of the folded fixed frame, a transverse lead screw is rotatably installed in the second U-shaped frame, a connecting arm is threadedly installed on the transverse lead screw, an installation frame is fixedly installed at the bottom of the connecting arm, a third telescopic cylinder is fixedly installed in the installation frame, the output shaft of the third telescopic cylinder extends below the installation frame and is fixedly installed with an adapter piece, the output shaft of the third telescopic cylinder is slidably connected to the bottom of the installation frame, a folding plate is fixedly installed at the bottom of the adapter piece, a resisting bar is fixedly installed on one side of the folding plate far from the connecting disk, a rodless cylinder is fixedly installed on one side of the folding plate far from the connecting disk, a linkage plate is fixedly installed on the slider of the rodless cylinder, two electric push rods are fixedly installed at the bottom of the linkage plate, and wedge-shaped inserting plates are fixedly installed on the output shafts of the two electric push rods.

[0008] Preferably, a reduction motor is fixedly installed on the inner wall of the top of the gantry, the output shaft of the reduction motor is fixedly connected to the bottom end of the rotating shaft, a servo motor is fixedly installed on the outer wall of one side of the second U-shaped frame, and the output shaft of the servo motor is fixedly connected to one end of the transverse lead screw.

[0009] Preferably, two side stops are fixedly installed on the top of the placing table, a deceleration belt is fixedly installed on the top of the placing table, and one side of the deceleration belt close to the support arm is flush with one side of the placing table close to the support arm.

[0010] Preferably, a fourth telescopic cylinder is fixedly installed on the outer wall of one side of the gantry, a moving column is fixedly installed on the output shaft of the fourth telescopic cylinder, and the top of the moving column is fixedly connected to the placing table.

[0011] Preferably, a guide rail is fixedly mounted on one side outer wall of the gantry, a guide block is slidably mounted on the guide rail, and the top of the guide block is fixedly connected to the movable column.

[0012] Preferably, the bottom of the first U-shaped frame contacts the support arm.

[0013] Compared with the related art, the protein blot loading device provided by the present invention has the following beneficial effects:

[0014] The utility model provides a protein blotting instrument loading device. By providing two groups of support arms and clamping parts on the support arms, when loading, the storage bottle whose analysis and detection has been completed previously can be clamped out first, and then the servo motor can be started to quickly alternate the two support arms, so that the next storage bottle can be quickly placed in the protein blotting instrument to continue analysis and detection. The loading speed is faster, and in the process of analyzing and detecting the sample in the previous storage bottle, the next storage bottle to be detected can be fixed first. When the analysis and detection of the previous sample is completed, the replacement can be quickly performed, and the time during the analysis process can be fully utilized, thereby further improving the analysis and detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 A schematic structural diagram of a preferred embodiment of the protein blot loading device provided by the present invention;

[0016] Figure 2 This is a schematic diagram of the front planar structure of the present utility model;

[0017] Figure 3 This is a schematic diagram of the assembly of the support arm and the first U-shaped frame in the present invention;

[0018] Figure 4 This is a schematic diagram of the connection structure between the display table and the side guard in the present utility model;

[0019] Figure 5 This is a schematic diagram of the assembly of the second U-shaped frame, the mounting frame and the folding plate in the present invention.

[0020] Numbers in the figure: 1. Gantry; 2. Rotating axis; 3. Connecting plate; 4. Support arm; 5. First telescopic cylinder; 6. First ⌚-shaped frame; 7. Second telescopic cylinder; 8. Clip; 9. Clamp; 10. Display table; 11. Side stop; 12. Folding fixing frame; 13. Second ⌚-shaped frame; 14. Transverse screw; 15. Connecting arm; 16. Mounting frame; 17. Third telescopic cylinder; 18. Clip; 19. Folding plate; 20. Contact strip; 21. Rodless cylinder; 22. Linkage plate; 23. Electric push rod; 24. Wedge-shaped insert; 25. Fourth telescopic cylinder; 26. Moving column. DETAILED DESCRIPTION

[0021] The present invention will be further described below with reference to the accompanying drawings and implementation examples.

[0022] Please refer to Figure 1-Figure 5 ,in, Figure 1 A schematic structural diagram of a preferred embodiment of the protein blot loading device provided by the present invention; Figure 2 This is a schematic diagram of the front planar structure of the present utility model; Figure 3 This is a schematic diagram of the assembly of the support arm and the first U-shaped frame in the present invention; Figure 4 This is a schematic diagram of the connection structure between the display table and the side guard in the present utility model; Figure 5This is an assembly schematic diagram of the second C-shaped frame, installation frame and folding plate in the present utility model. The protein blotting instrument feeding device includes: a gantry 1, a rotating shaft 2 is rotatably installed at the top of the gantry 1, and a reduction motor is fixedly installed on the inner wall of the top of the gantry 1, and its output shaft is fixedly connected to the bottom end of the rotating shaft 2. A connecting disk 3 is fixedly installed at the top end of the rotating shaft 2. Two support arms 4 are symmetrically arranged and fixedly installed on the outer wall of the connecting disk 3. First telescopic cylinders 5 are fixedly installed at the tops of the two support arms 4. First C-shaped frames 6 are fixedly installed on the output shafts of the two first telescopic cylinders 5. Second telescopic cylinders 7 are fixedly installed on the outer walls of both sides of the two first C-shaped frames 6, thus forming four second telescopic cylinders 7. The output shaft of each second telescopic cylinder 7 penetrates through the corresponding first C-shaped frame 6 and is slidably connected to the corresponding first C-shaped frame 6. A clamping piece 8 is fixedly installed on the output shaft of each second telescopic cylinder 7, thus forming four clamping pieces 8. Clamping blocks 9 are fixedly installed on the four clamping pieces 8, and the four clamping blocks 9 are adapted to each other. The bottom of the arranged first C-shaped frame 6 contacts the support arm 4, and a limiting rod is fixed inside the first C-shaped frame 6. The limiting rod penetrates through the clamping piece 8 and is slidably connected to it, so as to ensure that the first C-shaped frame 6 and the clamping block 9 can only move linearly. A fourth telescopic cylinder 25 is fixedly installed on the outer wall of one side of the gantry 1, and a moving column 26 is fixedly installed on its output shaft. A placing table 10 is fixedly installed on the top of the moving column 26. One section of the placing table 10 away from the support arm 4 is an inclined tabletop, and one section close to the support arm 4 is a horizontal tabletop. Two side blocks 11 are fixedly installed on the top of the placing table 10. The distance between the two side blocks 11 is adapted to the diameter of the storage bottle. When in use, multiple storage bottles are placed between the two side blocks 11, and most of the storage bottles are on the inclined tabletop, and only one storage bottle is on the horizontal tabletop. In this way, when the storage bottle on the horizontal tabletop is clamped away, the storage bottles on the inclined tabletop will automatically slide down for replacement, so as to facilitate clamping and use next time. And the horizontal tabletop is flush with the top tabletop of the support arm 4. A folding fixing frame 12 is fixedly installed on the top of the placing table 10. A second C-shaped frame 13 is fixedly installed on the inner wall of the top of the folding fixing frame 12. A transverse lead screw 14 is rotatably installed inside it, and a servo motor is fixedly installed on the outer wall of one side of the second C-shaped frame 13, and its output shaft is fixedly connected to one end of the transverse lead screw 14. A connecting arm 15 is threadedly installed on the transverse lead screw 14. An installation frame 16 is fixedly installed at the bottom of the connecting arm 15. A third telescopic cylinder 17 is fixedly installed inside the installation frame 16, and its output shaft extends below the installation frame 16 and is fixedly installed with an adapter piece 18. The output shaft of the third telescopic cylinder 17 is slidably connected to the bottom of the installation frame 16. A folding plate 19 is fixedly installed at the bottom of the adapter piece 18. A resisting strip 20 is fixedly installed on the side of the folding plate 19 away from the connecting disk 3. In the initial state, the storage bottle on the horizontal tabletop contacts the resisting strip 20, thus forming a block.A rodless cylinder 21 is fixedly mounted on the side of the folding plate 19 away from the connecting plate 3. A linkage plate 22 is fixedly mounted on the slider above it. Two electric push rods 23 are fixedly mounted on the bottom of the linkage plate 22. Wedge-shaped inserts 24 are fixedly mounted on the output shafts of the two electric push rods 23. The shortest distance between the wedge inserts 24 and the interference bar 20 matches the diameter of the storage bottle, so that a storage bottle can be cut out separately.

[0023] In the above method, in order to reduce the sliding speed and force of the storage bottle, a speed bump is fixedly installed on the top of the display table 10. The side of the speed bump close to the support arm 4 is flush with the side of the display table 10 close to the support arm 4. In the initial state, the bottom of the storage bottle on the horizontal surface contacts the speed bump, thereby preventing the storage bottle from sliding down.

[0024] In this method, in order to ensure that the display table 10 can slide stably in a straight line, a guide rail is fixedly installed on the outer wall of one side of the gantry 1, and a guide block is slidably installed on the guide rail, and the top of the guide block is fixedly connected to the movable column 26.

[0025] The working principle of the protein blot loading device provided by the utility model is as follows:

[0026] This device is used in conjunction with a protein blotting instrument. In the initial state, the output shaft of the third telescopic cylinder 17 is in an extended state. Before performing protein analysis, multiple storage bottles containing protein samples are first placed on the display table 10, and the spacing between the two side guards 11 is adapted to the diameter of the storage bottles, so that multiple storage bottles are placed in sequence. During the placement process, some storage bottles in the inclined section of the display table 10 will naturally slide down, so that the storage bottle closest to the support arm 4 will conflict with the interference bar 20, and the storage bottle will contact the speed bump on the display table 10. In addition, the shortest distance between the wedge-shaped insert 24 and the interference bar 20 is adapted to the diameter of the storage bottle.

[0027] When the protein blot instrument needs to be loaded during use, the rodless cylinder 21 is directly started, and the slider on it brings the linkage plate 22 down until it drops to the lowest position. Then, the output shafts of the two electric push rods 23 are started to extend, so that the two wedge-shaped inserts 24 approach each other and are finally inserted between the storage bottle at the most side and the storage bottle in contact with it, thereby separating the two storage bottles. At this time, there is only one storage bottle between the wedge-shaped insert 24 and the resistance bar 20. Then, the servo motor is started in the positive direction, and its output shaft drives the horizontal screw 14 to rotate, and the connecting arm 15 on it brings the mounting frame 16 water The connecting arm 15 moves horizontally, and at the same time, the storage bottle clamped by the wedge-shaped insert 24 and the interference bar 20 moves toward the corresponding support arm 4 until the connecting arm 15 moves to the most side position. At this time, the storage bottle between the wedge-shaped insert 24 and the interference bar 20 is between the two clamping blocks 9. Then, the output shafts of the corresponding two second telescopic cylinders 7 are extended to clamp the storage bottle. Then, the output shaft of the third telescopic cylinder 17 is retracted to move the interference bar 20 and the electric push rod 23 upward away from the storage bottle. Then, the output shaft of the fourth telescopic cylinder 25 is extended to leave space for the rotation of the support arm 4.

[0028] Subsequently, the reduction motor is started in the forward direction, which drives the rotating shaft 2 to rotate, and then the two supporting arms 4 are able to rotate. When the two supporting arms 4 rotate 180 degrees, the reduction motor is turned off, and the output shaft of the corresponding first telescopic cylinder 5 is immediately started to extend, and the clamped storage bottle is brought into the loading position in the protein blot instrument. Subsequently, the output shaft of the corresponding second telescopic cylinder 7 is started to retract, and the storage bottle is released, and the storage bottle is retained in the protein blot instrument. Subsequently, the output shaft of the corresponding first telescopic cylinder 5 is started to retract, and the clamping block 9 is brought back to its original position, and then the protein blot instrument is started for analysis.

[0029] During the analysis process, the output shaft of the fourth telescopic cylinder 25 is retracted to bring the placement table 10 back to its original position, and the servo motor is reversely started, and the rodless cylinder 21 is started to bring the contact bar 20 and the wedge-shaped insert 24 back to their original positions. At this time, when a storage bottle is pushed away, the remaining storage bottles continue to fill the position, so that the storage bottle at the most side contacts the contact bar 20 again, and then the storage bottle is brought into the two clamping blocks 9 in the same way as above, and is clamped by the clamping blocks 9;

[0030] After the previous analysis is completed, the output shaft of the corresponding first telescopic cylinder 5 is started to extend, and the corresponding two clamping blocks 9 are brought into the protein blot instrument to clamp the storage bottle. After that, the servo motor is started in the reverse direction, so that the two support arms 4 change positions again, and the next storage bottle can be quickly brought into the protein blot instrument for analysis. Then, this reciprocating process is repeated until all analyses are completed.

[0031] Compared with the related art, the protein blot loading device provided by the present invention has the following beneficial effects:

[0032] The utility model provides a protein blotting instrument loading device. By providing two groups of support arms 4 and clamping components on the support arms 4, when loading, the storage bottle whose analysis and detection has been completed previously can be clamped out first, and then the servo motor can be started to quickly alternate the two support arms 4, so that the next storage bottle can be quickly placed in the protein blotting instrument to continue analysis and detection. The loading speed is faster, and in the process of analyzing and detecting the sample in the previous storage bottle, the next storage bottle to be detected can be fixed first. When the analysis and detection of the previous sample is completed, the replacement can be quickly performed, and the time during the analysis process can be fully utilized, thereby further improving the analysis and detection efficiency.

[0033] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the description and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A protein blot loading device, comprising a gantry, characterized in that: A rotating shaft is rotatably installed at the top of the gantry. A connecting disc is fixedly installed at the top end of the rotating shaft. Two symmetrically arranged support arms are fixedly installed on the connecting disc. First telescopic cylinders are fixedly installed at the tops of the two support arms. First U-shaped frames are fixedly installed on the output shafts of the two first telescopic cylinders. Second telescopic cylinders are fixedly installed on the outer walls of both sides of the two first U-shaped frames. The output shafts of the four second telescopic cylinders respectively penetrate through the corresponding first U-shaped frames and are slidably connected to the corresponding first U-shaped frames. Clamping pieces are fixedly installed on the output shafts of the four second telescopic cylinders. Clamping blocks are fixedly installed on the four clamping pieces. The four clamping blocks are adapted to each other in pairs. A placement table is provided on one side of the gantry. One section of the placement table away from the support arm is an inclined tabletop, and the section close to the support arm is a horizontal tabletop. This horizontal tabletop is flush with the top tabletop of the support arm. A folded fixed frame is fixedly installed on the top of the placement table. A second U-shaped frame is fixedly installed on the inner wall of the top of the folded fixed frame. A transverse screw rod is rotatably installed in the second U-shaped frame. A connecting arm is threadedly installed on the transverse screw rod. An installation frame is fixedly installed at the bottom of the connecting arm. A third telescopic cylinder is fixedly installed in the installation frame. The output shaft of the third telescopic cylinder extends below the installation frame and is fixedly installed with an adapter piece. The output shaft of the third telescopic cylinder is slidably connected to the bottom of the installation frame. A folding plate is fixedly installed at the bottom of the adapter piece. A抵触条 is fixedly installed on the side of the folding plate away from the connecting disc. A rodless cylinder is fixedly installed on the side of the folding plate away from the connecting disc. A linkage plate is fixedly installed on the slider of the rodless cylinder. Two electric push rods are fixedly installed at the bottom of the linkage plate. Wedge-shaped insertion plates are fixedly installed on the output shafts of the two electric push rods.

2. The protein blot loading device according to claim 1, characterized in that: A reduction motor is fixedly installed on the inner wall of the top of the gantry. The output shaft of the reduction motor is fixedly connected to the bottom end of the rotating shaft. A servo motor is fixedly installed on the outer wall of one side of the second U-shaped frame. The output shaft of the servo motor is fixedly connected to one end of the transverse screw rod.

3. The protein blot loading device according to claim 1, characterized in that: Two side stops are fixedly installed on the top of the placement table. A deceleration belt is fixedly installed on the top of the placement table. The side of the deceleration belt close to the support arm is flush with the side of the placement table close to the support arm.

4. The protein blot loading device according to claim 1, characterized in that: A fourth telescopic cylinder is fixedly installed on the outer wall of one side of the gantry. A moving column is fixedly installed on the output shaft of the fourth telescopic cylinder. The top of the moving column is fixedly connected to the placement table.

5. The protein blot loading device according to claim 4, characterized in that: A guiding slide rail is fixedly installed on the outer wall of one side of the gantry. A guiding block is slidably installed on the guiding slide rail. The top of the guiding block is fixedly connected to the moving column.

6. The protein blot loading device according to claim 1, characterized in that: The bottom of the first U-shaped frame contacts the support arm.

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