Biological sample low-temperature preservation device

Abstract

The utility model discloses a biological sample low-temperature preservation device. A freezing storage set and a rotating assembly are placed in a low-temperature storage cavity; the bottom of the lifting part is arranged at the top, located in the transition area, of the heat preservation tank body, a lifting assembly is arranged at the top of the lifting part, and a lifting part of the lifting assembly can sequentially penetrate through the heat preservation taking and placing part and the transition part and extend into the heat preservation part; the first liquid level sensor, the liquid inlet pipeline, the first temperature sensor and the second liquid level sensor are all located in the heat preservation tank body, the first liquid level sensor and the liquid inlet pipeline are both close to the lower surface of the rotating disc, the first temperature sensor is located on the peripheral wall face of the rotating disc, and the second liquid level sensor is located on the inner top of the heat preservation tank body. The liquid inlet valve is communicated with the liquid supplementing valve, the liquid supplementing valve is communicated with the liquid inlet pipeline, the exhaust valve is communicated between the liquid supplementing valve and the liquid inlet valve, and the exhaust valve is communicated with the vertical channel; the second temperature sensor is located in the cylinder. When the cryopreservation box is taken and placed, the cryopreservation box is in a low-temperature area of-80 DEG C to-170 DEG C, so that the biological sample is always in a protection state.

Description

Technical Field

[0001] The utility model relates to the technical field of biological sample freezing and preservation, in particular to a biological sample low-temperature preservation device. Background Art

[0002] Cryopreservation of biological specimens (such as cells, tissues, blood, sperm, eggs, and embryos) involves freezing them using cryogenic technology and storing them under appropriate conditions for a long period of time, ensuring they retain their vitality or functionality after thawing. Cryopreservation has a wide range of applications in medicine, agriculture, environmental protection, and scientific research. The fundamental principle of cryopreservation is to use low temperatures to slow the rate of biochemical reactions within and between cells, preventing degradation of the specimen during storage. Cryopreservation also slows the growth of pathogens, extending the shelf life of biological specimens.

[0003] In the prior art, there is a biological sample freezing storage device and a biological sample moving method with the publication number CN110654721 B. The biological sample freezing storage device includes a storage container and an access device fixed to the storage container. The storage container includes a tank body capable of containing a cryopreservative liquid and a container opening located at the upper portion of the tank body; and the access device includes a column, a cross arm portion, and a biological sample extraction portion. The column is provided with a vertical moving portion, which can move in the vertical direction along the column, and the cross arm portion is provided with a horizontal moving portion, which can move in the horizontal direction along the cross arm portion. The cross arm portion is provided on the vertical moving portion, so that the cross arm portion can move up and down in the vertical direction. The biological sample extraction portion is provided on the horizontal moving portion, so that the biological sample extraction portion can move in the horizontal direction.

[0004] However, the existing technology cannot solve the problem of realizing the functions of rotating the liquid nitrogen freezing rack and assisting in lifting the liquid nitrogen freezing rack. Utility Model Content

[0005] In response to the above technical problems, the present application solves the problem in the prior art that the functions of rotating the liquid nitrogen freezing rack and assisting in lifting the liquid nitrogen freezing rack cannot be solved.

[0006] In order to achieve the above-mentioned purpose, the technical solution adopted in the present application is as follows: a biological sample low-temperature storage device, comprising a heat preservation part, wherein the heat preservation part has a low-temperature storage cavity, wherein the low-temperature storage cavity contains multiple groups of frozen storage groups for preserving biological samples and a rotating assembly that drives the multiple groups of frozen storage groups to perform intermittent rotational movement as a whole; the transition part has a transition area located around the top of the heat preservation part, and the transition area is provided with a driving assembly for driving the rotating assembly; the heat preservation and placement part has a placement cavity, and the bottom opening of the placement cavity is connected to the top of the low-temperature storage cavity; the bottom of the lifting part is provided at the top of the heat preservation tank body located in the transition area, and the top of the lifting part is provided with a lifting assembly that can move horizontally linearly, and the lifting part of the lifting assembly can pass through the heat preservation and placement part and the transition part in sequence and extend into the heat preservation part;

[0007] The device further includes the first liquid level sensor, the liquid inlet pipe, the first temperature sensor, and the second liquid level sensor located within the heat-insulating tank body, wherein the first liquid level sensor and the liquid inlet pipe are both located near the lower surface of the turntable, the first temperature sensor is located on the outer peripheral wall of the turntable, and the second liquid level sensor is located on the inner top of the heat-insulating tank body;

[0008] The liquid replenishing valve, liquid inlet valve and exhaust valve are all located on the peripheral wall of the heat-insulating tank body, the liquid inlet valve is connected to the liquid replenishing valve, the liquid replenishing valve is connected to the liquid inlet pipe, the exhaust valve is connected between the liquid replenishing valve and the liquid inlet valve, and the exhaust valve is connected to the vertical channel;

[0009] The second temperature sensor is located in the barrel.

[0010] In order to better realize the present invention, further, the heat preservation part includes a heat preservation tank body, the heat preservation tank body has a low-temperature storage cavity, the top of the low-temperature storage cavity is provided with a heat preservation tank body sampling port, and the heat preservation tank body sampling port is placed with a heat preservation plug cover B and a heat preservation plug cover A;

[0011] The rotating assembly includes a turntable and a rotating shaft. The turntable is arranged at the lower section of the rotating shaft. A frozen storage group is placed on the turntable. The bottom rotation of the rotating shaft is arranged at the center position of the insulation tank base at the bottom of the insulation tank body, and the top rotation of the rotating shaft is arranged at the top of the insulation tank body.

[0012] In order to better realize the present invention, further, the contact surface between the thermal insulation plug body cover B and the thermal insulation plug body cover A is a stepped surface.

[0013] In order to better implement the present invention, further, the frozen storage group includes a liquid nitrogen freezing rack, the area of ​​the heat-insulating tank body located above the turntable forms a plurality of mutually independent freezing placement areas, each freezing placement area is placed with a plurality of the liquid nitrogen freezing racks, and a plurality of freezing boxes are vertically arranged on the liquid nitrogen freezing rack.

[0014] In order to better realize the present invention, the driving assembly further includes a reducer, a chain, a driven wheel, a motor and a driving wheel.

[0015] The reducer and the motor are both arranged at the top of the heat-insulating tank body in the transition area. The input end of the reducer is connected to the output end of the motor. The output end of the reducer is provided with a driving wheel. The top of the rotating shaft extending out of the heat-insulating tank body is provided with a driven wheel. The driven wheel and the driving wheel are linked to each other by a chain.

[0016] In order to better realize the present utility model, further, the heat-insulating taking-and-putting part includes a cylinder, the cylinder has a taking-and-putting cavity, the top of the cylinder is provided with a cylinder top opening, a plug cover is placed on the cylinder top opening, a plug cover strip seam is provided in the middle of the plug cover, a spray assembly is provided on the inner wall surface of the upper section of the taking-and-putting cavity, and a taking-and-putting window assembly is provided in the middle and lower sections of the taking-and-putting cavity.

[0017] The spray assembly includes a nozzle, an annular channel and a vertical channel. A plurality of output holes are formed in a ring shape on the lower side of the annular channel, each of which is provided with a nozzle. An input hole is formed on the upper side of the annular channel, and the input hole is connected to the output end of the vertical channel.

[0018] The access window assembly includes an access door, a movable door, a strip-shaped observation hole, a limit guide strip, a vertical slide groove, a vertical slider and a locking bolt. The access window is opened in the middle and lower sections of the access cavity. A connecting piece is provided on the outer peripheral wall surface of the cylinder above the access window. Limit guide strips are provided on the left and right sides of the access window.

[0019] The retrieval window is provided with a retrieval door that can be flipped open and closed, and the movable door is located between the cylinder and the retrieval door, and the left and right side edges of the movable door respectively correspond to the inner side surface of the limiting guide strip for sliding fit, and a vertical slide groove is vertically opened at the middle position of the movable door, and the sliding sleeve in the vertical slide groove is provided with the connecting piece, and the slide groove part of the vertical slide groove above the connecting piece is provided with a vertical slider through a locking bolt sliding sleeve, and the vertical movement of the vertical slider can be locked by the locking bolt, and the movable door is provided with a strip-shaped observation hole at the position below the vertical slide groove.

[0020] In order to better realize the present invention, further, the lifting part includes a lifting support frame, the heat preservation tank body of the lifting support frame is located at the top of the transition area, and a lifting assembly is provided on the top of the lifting support frame. The lifting assembly includes an aluminum plate, a slide, a guide rail, a strip aluminum profile, a lifting frame, a side slider, a rotating wheel, a lifting motor, a chain rope, a hook, a wire trough support frame, a tank chain wire trough, a ring chain and a driving motor.

[0021] The aluminum plate is fixedly arranged on the top of the lifting support frame, the upper surface of the aluminum plate is provided with a slide groove, a guide rail is slidably arranged in the slide groove, the upper surface of the guide rail is provided with a strip aluminum profile, one end of the strip aluminum profile is provided with a lifting frame, a rotating wheel is provided in the lifting frame through a first rotating shaft, one end of the first rotating shaft is connected to the lifting motor, one end of the chain lifting rope is provided on the rotating wheel, and the other end of the chain lifting rope is provided with a hook.

[0022] A wire trough support frame is provided on the upper surface of the aluminum plate on one side of the slide trough, the tank chain wire trough is provided on the upper surface of the wire trough support frame, a ring chain is provided in the tank chain wire trough, a sprocket is provided on the upper surface of the wire trough support frame away from the tank chain wire trough for rotation via a second rotating shaft, the part of the chain extending out of the tank chain wire trough is linked to the sprocket, and the drive motor is connected to one end of the second rotating shaft.

[0023] In order to better implement the present invention, the storage device further includes a laser locator, which is arranged on one side of the lifting frame.

[0024] In order to better realize the present invention, further, a step rack is provided on one side of the bottom of the heat-insulating tank body, and an endoscope is placed on the top of the heat-insulating tank body in the transition area.

[0025] Compared with the prior art, the technical solution provided by the present invention has the following beneficial effects:

[0026] 1. The utility model achieves cryogenic storage of biological samples through the coordinated design of the heat preservation portion, transition portion, heat preservation access portion, and lifting portion. On the one hand, it also eliminates the need to manually rotate the liquid nitrogen freezing rack to place the tray. The internal spray cooling of the heat preservation box keeps the cryobox in a low temperature range of -80°C to -170°C during storage and retrieval, keeping the samples in a protected state. The liquid nitrogen freezing rack is also lifted to assist in lifting.

[0027] 2. The utility model rotates the freezing placement area through the coordinated design of the driving component and the rotating component, so that the liquid nitrogen freezing rack where the pre-taken biological sample is located is rotated to the bottom of the sampling port of the insulation tank, which is convenient for taking and placing.

[0028] 3. The present invention facilitates taking of the heat preservation plug body cover B and the heat preservation plug body cover A by designing the contact surface between the heat preservation plug body cover B and the heat preservation plug body cover A as a stepped surface.

[0029] 4. The utility model is adapted to different biological samples by designing different freezing placement areas.

[0030] 5. The utility model provides a larger space for the thermal insulation tank body by locating the driving component in the transition area.

[0031] 6. The utility model ensures that the biological sample remains in an insulated state during the retrieval process through the design of the cylinder, the retrieval door, the top opening of the cylinder, the plug cover, the strip slit of the plug cover, the movable door, the strip observation hole, the limit guide strip, the nozzle, the annular channel, the vertical channel, the vertical slide groove, the vertical slider and the locking bolt.

[0032] 7. The utility model assists operators in extracting liquid nitrogen freezing racks through the coordinated design of the lifting part.

[0033] 8. The utility model uses a laser locator design and is used in conjunction with the lifting part to locate the top lifting position of the liquid nitrogen freezing rack in a mist state, making it convenient to hang it with a hook.

[0034] 9. The utility model provides the operator with sufficient height to operate at the movable door through the design of the ladder rack. The design of the endoscope, based on the laser locator, further facilitates the operator to identify the required position of the liquid nitrogen freezing rack. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without paying any creative labor.

[0036] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0037] Figure 2 for Figure 1 Front view of

[0038] Figure 3 for Figure 2 Cross-sectional view at AA in the middle;

[0039] Figure 4 for Figure 1 The schematic diagram of the structure of the cylinder in the heat preservation taking and placing part is removed;

[0040] Figure 5 for Figure 4 Cross-sectional view at the middle BB;

[0041] Figure 6 for Figure 1 A three-dimensional diagram of the cylinder in the heat preservation and placement part is removed

[0042] Figure 7 for Figure 6 Enlarged view of point C in the middle;

[0043] Figure 8 This is a structural diagram without the middle part of the insulation tank;

[0044] Figure 9 for Figure 8 Enlarged view of point D in the middle;

[0045] Figure 10 This is a schematic structural diagram of the plug body cover in the utility model;

[0046] Figure 11 This is a schematic diagram of the cooperation between the insulation plug body cover A and the insulation plug body cover B in the present invention;

[0047] Figure 12 This is a schematic structural diagram of the liquid nitrogen freezing rack in the present invention.

[0048] In the figure: 10-insulation part; 20-transition part; 30-insulation taking and placing part; 40-lifting part; 50-control part; 101-insulation tank body; 102-insulation tank body sampling port; 103-insulation plug cover B; 104-insulation plug cover A; 105-insulation tank body base; 106-turntable; 107-liquid nitrogen freezing rack; 108-first liquid level sensor; 109-liquid inlet pipe; 110-first temperature sensor; 111-second liquid level sensor; 112-liquid replenishing valve; 113-liquid inlet valve; 114-exhaust valve; 115-rotating shaft; 116-ladder rack; 2 01-reducer; 202-chain; 203-driven wheel; 204-motor; 205-driving wheel; 301-cylinder; 302-pick-and-place door; 303-top opening of cylinder; 304-plug cover; 305-slit of plug cover; 306-movable door; 307-strip observation hole; 308-limiting guide strip; 309-nozzle; 310-annular channel; 311-vertical channel; 312-vertical slide; 313-vertical slider; 314-locking bolt; 315-connecting piece; 401-lifting support frame; 402-chain lifting rope; 501-endoscope. DETAILED DESCRIPTION

[0049] To make the objectives, technical solutions, and advantages of the embodiments of the present application more clear, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Generally, the components of the embodiments of the present application described and shown in the drawings herein can be arranged and designed in various different configurations.

[0050] Therefore, the following detailed description of the embodiments of the present application provided in the accompanying drawings is not intended to limit the scope of the present application for protection, but merely represents selected embodiments of the present application. All other embodiments obtained by persons of ordinary skill in the art based on the embodiments in the present application without making any creative efforts shall fall within the scope of protection of the present application.

[0051] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined or explained in subsequent drawings.

[0052] In the description of this application, it should be noted that if the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. appear, the orientation or position relationship indicated is based on the orientation or position relationship shown in the accompanying drawings, or the orientation or position relationship in which the product of the application is usually placed when in use. It is only for the convenience of describing this application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation. Therefore, it cannot be understood as a limitation on this application. In addition, if the terms "first", "second", etc. appear in the description of this application, they are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0053] Furthermore, the use of terms such as "horizontal" and "vertical" in the description of this application does not necessarily imply that a component must be absolutely horizontal or suspended, but rather that it can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical" and does not mean that the structure must be completely horizontal, but rather that it can be slightly tilted.

[0054] It should also be noted that, unless otherwise expressly specified or limited, the terms "disposed," "installed," "connected," and "connected" should be understood broadly. For example, they may refer to fixed connections, detachable connections, or integral connections; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal connections between two components. A person of ordinary skill in the art will understand the specific meanings of the above terms in this application based on the specific circumstances.

[0055] Example 1

[0056] like Figure 1 As shown, a biological sample cryopreservation device includes a heat preservation portion 10, wherein the heat preservation portion 10 has a cryopreservation cavity, wherein the cryopreservation cavity contains multiple groups of frozen storage groups for storing biological samples and a rotating assembly for driving the multiple groups of frozen storage groups to perform intermittent rotational motion;

[0057] The transition portion 20 has a transition area located around the top access opening of the heat preservation portion 10, and a driving assembly for driving the rotating assembly is provided in the transition area;

[0058] The heat-insulating access portion 30 has an access cavity therein, and the bottom opening of the access cavity is connected to the top of the low-temperature storage cavity;

[0059] The bottom of the lifting portion 40 is arranged at the top of the heat-insulating tank body 101 in the transition area. A lifting assembly that can move horizontally and linearly is provided on the top of the lifting portion 40. The lifting portion of the lifting assembly can sequentially pass through the heat-insulating loading and unloading portion 30 and the transition portion 20 and extend into the heat-insulating portion 10.

[0060] The heat-insulating tank 101 further includes a first liquid level sensor 108, a liquid inlet pipe 109, a first temperature sensor 110, and a second liquid level sensor 111. The first liquid level sensor 108 and the liquid inlet pipe 109 are both located near the lower surface of the turntable 106 (the distance between the liquid inlet pipe 109 and the lower surface of the turntable 106 is 10 mm to 50 mm). The first temperature sensor 110 is located on the outer peripheral wall of the turntable 106. The second liquid level sensor 111 is located on the inner top of the heat-insulating tank 101.

[0061] The liquid replenishing valve 112, the liquid inlet valve 113 and the exhaust valve 114 are all located on the outer peripheral wall of the heat-insulating tank body 101. The liquid inlet valve 113 is in communication with the liquid replenishing valve 112, and the liquid replenishing valve 112 is in communication with the liquid inlet pipe 109. The exhaust valve 114 is in communication between the liquid replenishing valve 112 and the liquid inlet valve 113, and the exhaust valve 114 is in communication with the vertical channel 311.

[0062] The second temperature sensor is located in the barrel 301 .

[0063] Through the coordinated design of the heat preservation part 10, the transition part 20, the heat preservation taking and placing part 30, and the lifting part 40, on the one hand, the low temperature preservation of the biological sample is achieved; on the other hand, there is no need to manually rotate the liquid nitrogen freezing rack 107 to place the tray; the internal temperature of the heat preservation box is sprayed to reduce the temperature so that the cryopreserved box is kept at -80°C during the storage and retrieval process.

[0064] -170℃ low temperature area keeps samples in a protected state at all times; auxiliary lifting liquid nitrogen freezing rack.

[0065] like Figure 1 、 Figure 2 、 Figure 4 、 Figure 5 、 Figure 6 and Figure 8 As shown, in this embodiment, the heat preservation part 10 includes a heat preservation tank body 101, and the heat preservation tank body 101 has a low-temperature storage cavity. The top of the low-temperature storage cavity is provided with a heat preservation tank body sampling port 102, and the heat preservation tank body sampling port 102 is placed with a heat preservation plug cover B103 and a heat preservation plug cover A104;

[0066] The rotating assembly includes a turntable 106 and a rotating shaft 115. The turntable 106 is arranged at the lower section of the rotating shaft 115. A frozen storage group is placed on the turntable 106. The bottom rotation of the rotating shaft 115 is arranged at the center position of the insulation tank base 105 at the bottom of the insulation tank body 101, and the top rotation of the rotating shaft 115 is arranged at the top of the insulation tank body 101.

[0067] By coordinating the design of the driving assembly and the rotating assembly, the freezing placement area is rotated so that the liquid nitrogen freezing rack 107 where the pre-taken biological sample is located is rotated to just below the sampling port 102 of the insulation tank body, making it easy to take and place.

[0068] like Figure 8 and Figure 11 As shown, in this embodiment, the contact surface between the thermal insulation plug body cover B103 and the thermal insulation plug body cover A104 is a stepped surface.

[0069] The contact surface between the thermal insulation plug cover B103 and the thermal insulation plug cover A104 is designed as a stepped surface, which makes it easy to take.

[0070] like Figure 2 、 Figure 5 、 Figure 6 、 Figure 8 and Figure 12 As shown, in this embodiment, the frozen storage group includes a liquid nitrogen freezing rack 107, and the area of ​​the heat-insulating tank body 101 located above the turntable 106 forms a plurality of mutually independent freezing placement areas, each of which is provided with a plurality of the liquid nitrogen freezing racks 107, and a plurality of freezing boxes are vertically arranged on the liquid nitrogen freezing rack 107.

[0071] By designing different freezing storage areas, it can adapt to different biological samples.

[0072] like Figure 5As shown, in this embodiment, the driving assembly includes a reducer 201, a chain 202, a driven wheel 203, a motor 204 and a driving wheel 205.

[0073] The reducer 201 and the motor 204 are both arranged at the top of the heat-insulating tank body 101 in the transition area. The input end of the reducer 201 is connected to the output end of the motor 204. The output end of the reducer 201 is provided with a driving wheel 205. The top of the rotating shaft 115 extending out of the heat-insulating tank body 101 is provided with a driven wheel 203. The driven wheel 203 and the driving wheel 205 are linked to each other by a chain 202.

[0074] The design of locating the driving assembly in the transition area allows the heat-insulating tank body 101 to have a larger space.

[0075] like Figure 1 、 Figure 3 、 Figure 4 、 Figure 6 、 Figure 7 、 Figure 8 As shown in FIG. 10 , in this embodiment, the heat-insulating taking-and-placing portion 30 includes a cylinder 301, the cylinder 301 having a taking-and-placing cavity, a cylinder top opening 303 being provided on the top of the cylinder 301, a plug cover 304 being placed on the cylinder top opening 303, a plug cover strip slit 305 being provided in the middle of the plug cover 304, a spray assembly being provided on the inner wall surface of the upper section of the taking-and-placing cavity, and a taking-and-placing window assembly being provided in the middle and lower sections of the taking-and-placing cavity.

[0076] The spray assembly includes a nozzle 309, an annular channel 310, and a vertical channel 311. The annular channel 310 has a plurality of output holes formed in a circular shape on its lower side, each of which is provided with a nozzle 309. The annular channel 310 has an input hole formed on its upper side, which is connected to the output end of the vertical channel 311.

[0077] The access window assembly includes an access door 302, a movable door 306, a strip-shaped observation hole 307, a limiting guide strip 308, a vertical slide groove 312, a vertical slider 313, and a locking bolt 314. The access window is defined in the middle and lower sections of the access cavity. A connecting piece 315 is provided on the outer peripheral wall of the cylinder 301 above the access window. Limiting guide strips 308 are provided on the left and right sides of the access window.

[0078] The pick-and-place window is provided with a pick-and-place door 302 that can be flipped open and closed, and the movable door 306 is located between the cylinder 301 and the pick-and-place door 302. The left and right side edges of the movable door 306 respectively correspond to the inner side surfaces of the limiting guide strips 308 for sliding fit, and a vertical sliding groove 312 is vertically opened at the middle position of the movable door 306. The vertical sliding groove 312 is slidably sleeved with the connecting piece 315. The sliding groove part of the vertical sliding groove 312 located above the connecting piece 315 is slidably sleeved with a vertical slider 313 through a locking bolt 314, and the vertical movement of the vertical slider 313 can be locked by the locking bolt 314. The movable door 306 is provided with a strip-shaped observation hole 307 at a position below the vertical sliding groove 312.

[0079] Through the design of the cylinder 301, the taking and placing door 302, the top opening 303 of the cylinder, the plug cover 304, the plug cover strip slit 305, the movable door 306, the strip observation hole 307, the limiting guide strip 308, the nozzle 309, the annular channel 310, the vertical channel 311, the vertical slide groove 312, the vertical slider 313 and the locking bolt 314, the biological sample is ensured to remain in an insulated state during the taking and placing process.

[0080] like Figure 1 、 Figure 2 and Figure 8 As shown, in this embodiment, the lifting part 40 includes a lifting support frame 401, and the heat-insulating tank body 101 is located at the top of the lifting support frame 401 in the transition area. A lifting component is provided on the top of the lifting support frame 401, and the lifting component includes an aluminum plate, a slide, a guide rail, a strip aluminum profile, a lifting frame, a side slider, a rotating wheel, a lifting motor, a chain rope 402, a hook, a wire trough support frame, a tank chain wire trough, a ring chain and a driving motor.

[0081] The aluminum plate is fixedly arranged on the top of the lifting support frame 401, and a slide groove is arranged on the upper surface of the aluminum plate, and a guide rail is slidingly arranged in the slide groove. A strip aluminum profile is arranged on the upper surface of the guide rail, and a lifting frame is arranged at one end of the strip aluminum profile. A rotating wheel is arranged in the lifting frame to rotate through a first rotating shaft, and one end of the first rotating shaft is connected to the lifting motor. One end of the chain rope 402 is arranged on the rotating wheel, and the other end of the chain rope 402 is provided with a hook.

[0082] A wire trough support frame is provided on the upper surface of the aluminum plate on one side of the slide trough, the tank chain wire trough is provided on the upper surface of the wire trough support frame, a ring chain is provided in the tank chain wire trough, a sprocket is provided on the upper surface of the wire trough support frame away from the tank chain wire trough for rotation via a second rotating shaft, the part of the chain extending out of the tank chain wire trough is linked to the sprocket, and the drive motor is connected to one end of the second rotating shaft.

[0083] The coordinated design of the lifting portion 40 assists the operator in extracting the liquid nitrogen freezing rack 107 .

[0084] In this embodiment, the storage device further includes a laser locator, which is arranged on one side of the lifting frame.

[0085] Through the design of the laser locator, used in conjunction with the lifting portion 40, the top lifting position of the liquid nitrogen freezing rack 107 is located in the mist state, making it convenient to hang it with the hook.

[0086] like Figure 1 As shown, in this embodiment, a stepped rack 116 is further provided on one side of the bottom of the heat-insulating tank body 101, and an endoscope 501 is further placed on the top of the heat-insulating tank body 101 in the transition area.

[0087] The design of the ladder rack 116 provides the operator with sufficient height to operate at the movable door 306. The design of the endoscope 501, based on the laser locator, further facilitates the operator to identify the required position of the liquid nitrogen freezing rack 107.

[0088] Example 2

[0089] like Figure 1 As shown, based on the first embodiment, it further includes a control unit 50, which is used to control the rotation of the rotating component through the driving component, to control the horizontal linear movement of the lifting component, and to control the vertical lifting of the lifting part.

[0090] The control unit 50 includes a controller,

[0091] When the controller receives a signal that a designated frozen storage area is located below the sampling port 102 of the heat preservation tank, the controller sends a start signal to a first execution element that controls the opening and closing of the motor 204 (for example, turns on a first relay between the motor 204 and an external power source), so that the motor 204 and the external power source are in a conducting state, thereby rotating the turntable 106; when the frozen storage area is located below the sampling port 102 of the heat preservation tank, the controller sends a shut-down signal to the first execution element that controls the opening and closing of the motor 204, so that the motor 204 and the external power source are in a disconnected state;

[0092] When the controller receives a signal to horizontally move the lifting assembly, the controller sends a start signal to a second actuator that controls the start and stop of the driving motor of the lifting assembly (for example, turning on a second relay between the driving motor and an external power supply), so that the driving motor and the external power supply are in a conducting state, causing the lifting assembly to move horizontally; when the lifting assembly moves to a specified position, the controller sends a shutoff signal to the second actuator that controls the start and stop of the driving motor of the lifting assembly, so that the driving motor and the external power supply are in a disconnected state;

[0093] When the controller receives a signal causing the hook of the lifting assembly to be raised or lowered, the controller sends a start signal to a third executive element that controls the opening and closing of the lifting motor of the lifting assembly (for example, turning on a third relay between the lifting motor and an external power supply), so that the lifting motor and the external power supply are in a conducting state, causing the hook of the lifting motor to rise or fall; when the hook of the lifting motor rises or falls to a specified position, the controller sends a shut-down signal to the third executive element that controls the opening and closing of the lifting motor, so that the rising or falling hook of the lifting motor is disconnected from the external power supply;

[0094] The controller receives a first liquid level signal detected by the first liquid level sensor 108 and converts the first liquid level signal into a first digital signal. When the first digital signal is less than a preset liquid replenishment starting value, the controller sends a first control signal to the liquid replenishment valve 112 and the liquid inlet valve 113. The liquid replenishment valve 112 and the liquid inlet valve 113 are opened, so that the external liquid is replenished to the bottom of the thermal insulation tank body 101 through the liquid inlet pipe 109.

[0095] The controller receives a first temperature signal or a second temperature signal corresponding to the first temperature sensor 110 or the second temperature sensor, and converts the first temperature signal or the second temperature signal into a second digital signal or a third digital signal respectively. When one of the second digital signal or the third digital signal is greater than the exhaust set value preset in the corresponding control unit, the controller sends a second control signal to the liquid inlet valve 113 and the exhaust valve 114, and the liquid inlet valve 113 is closed and the exhaust valve 114 is opened. Otherwise, the controller sends a third control signal to the liquid inlet valve 113 and the exhaust valve 114, and the liquid replenishing valve 112 and the liquid inlet valve 113 are opened, and the exhaust valve 114 is closed.

[0096] The controller receives the second liquid level signal detected by the first liquid level sensor 108 and converts the second liquid level signal into a second digital signal. When the second digital signal is greater than a preset refill stop value, the controller sends a fourth control signal to the refill valve 112, the liquid inlet valve 113 and the exhaust valve 114, and the refill valve 112, the liquid inlet valve 113 and the exhaust valve 114 are closed.

[0097] Through the design of the control unit 50, on the one hand, the automatic rotation of the liquid nitrogen freezing rack 107 in the insulation part 10 is guaranteed, the horizontal sliding of the lifting part 40 and the automatic lifting and lowering of the lifting components in the lifting part 40 are guaranteed; on the other hand, the automatic implementation of liquid intake, liquid replenishment and exhaust is guaranteed.

[0098] In addition, the control unit 50 is further provided with a touch screen, and the touch screen is connected to the controller.

[0099] Automatic mode rehydration can be achieved:

[0100] Operations before starting automatic rehydration: pre-set the rehydration start value and rehydration stop value;

[0101] After setting, click [Automatic Mode] to turn on the automatic refill mode through the control of the controller;

[0102] When the liquid level is lower than the refill starting value, the automatic refill mode is turned on.

[0103] STEP 1: When the liquid level is lower than the starting value for rehydration, open the [liquid inlet valve];

[0104] STEP2: When the exhaust temperature is higher than the exhaust setting value, open the exhaust valve and enter the exhaust process;

[0105] The time / temperature of the exhaust process can be set as needed in the parameter settings;

[0106] When the time is reached or the exhaust temperature is ≤ the set exhaust temperature, the exhaust process ends;

[0107] STEP 3: After the exhaust process is completed, close the exhaust valve and open the rehydration valve to enter the rehydration process;

[0108] When the rehydration stop value is reached, rehydration stops. The rehydration start and stop values ​​are set in the parameter settings.

[0109] Effect achieved: Automatic rehydration can be achieved in automatic mode, and rehydration will be automatically performed as the liquid nitrogen is consumed.

[0110] During demisting, liquid nitrogen is injected into the heat-insulating tank body 101 or sprayed through the nozzle 309 .

[0111] During gas phase storage, if a malfunction or accident causes the liquid nitrogen storage volume to exceed the lowest frozen sample position (i.e., exceed the height of the turntable 106),

[0112] The protection program is started to stop the automatic filling of liquid nitrogen and start the alarm (the control part also includes an alarm device, and the alarm device is connected to the controller).

[0113] Conditions to determine if liquid nitrogen is too high:

[0114] Condition 1: Liquid level height > set maximum liquid level height value;

[0115] Condition 2: The lower temperature exceeds the lower limit of the lower temperature -195°C;

[0116] When one of the above conditions is triggered, all valves are forced to close, and the inlet valve and the replenishing valve cannot be opened again, whether manually or automatically.

[0117] Preset the insulation chamber cooling start temperature and insulation chamber cooling shut-off temperature;

[0118] Then click the "Insulation Chamber Cooling" button on the control screen homepage;

[0119] After the insulation chamber cooling is turned on, the following control process can also be performed:

[0120] Step 1: First open the [liquid inlet valve] + [exhaust valve] to exhaust.

[0121] Step 2: After the exhaust temperature is reached, close the exhaust valve; open the insulation chamber cooling solenoid valve (hereinafter referred to as the cooling valve) and then determine whether the main tank liquid level needs to be replenished; if the main tank liquid level is above the set replenishment starting value, do not open the replenishment valve:

[0122] If it is not above the rehydration start value, the tank body will be triggered to automatically rehydrate (i.e. open the rehydration valve of the main tank, and close the rehydration valve after reaching the rehydration stop value).

[0123] Step 3: As liquid nitrogen is sprayed, the temperature of the insulation chamber decreases. When the temperature of the insulation chamber reaches the set value (insulation chamber cooling shut-off temperature), the cooling valve is closed after the delayed spraying time (seconds):

[0124] Step 4: After the refrigeration valve is closed, as the temperature of the insulation chamber rises to above the refrigeration opening temperature of the insulation chamber, the refrigeration valve is opened again to spray: maintain the temperature in the insulation chamber.

[0125] Step 5: Until the user opens the door of the insulation chamber (door switch signal), or closes the insulation chamber on the screen

[0126] Press the refrigeration button to stop the above refrigeration and heat preservation process, reset all solenoid valves, and open the door to reset the refrigeration button of the heat preservation chamber.

[0127] Sample access operation flow

[0128] 1. Manually open the insulation box door (i.e. the insulation box is the cylinder 301, and the insulation box door is the access door 302 and the movable door 306 at the access window). The box opening size is 275mm wide * 4 insulation access parts 30mm high.

[0129] (Freezing rack height is about 660mm)

[0130] 2. Manually remove the wide-neck plug cover. The plug cover is divided into two parts, A and B. Remove the A and B covers in order.

[0131] 3. Click the access button on the touch screen to select a storage area (or a frozen storage area, for example, frozen storage area A, frozen storage area B, frozen storage area C, frozen storage area D), so that a frozen storage area is rotated to the bottom of the sampling port 102 of the insulation tank.

[0132] 4. Press and hold the lifting hook down button (physical button) to lower the lifting hook to the appropriate position. Slide the lifting hook back and forth to the cryostat and hook it onto the cryostat. (The height between the top of the cryostat and the sampling port is approximately 580 mm).

[0133] 5. Close the incubator door

[0134] Set the required temperature for insulation on the touch screen and click the cooling button to cool.

[0135] After reaching the cooling temperature, click the touch screen to turn off the cooling button and open the insulation box door (the controller is also connected to the cooling solenoid valve, which is forced to close after the door is opened, which is an existing mature technical means).

[0136] 7. Press the lifting button (physical button) to raise the lifting hook to the sampling door.

[0137] Manually remove and place the cryopreservation boxes. (The cryopreservation boxes need to be manually located on the shelf.)

[0138] 8. After sampling, return the cryopreservation rack to the storage area, cover it with the wide-necked plug cap, and place it in order.

[0139] Put on covers B and A.

[0140] 9. Close the insulation box door, and the entire sampling process is completed.

[0141] The above description is merely a preferred embodiment of the present application and is not intended to limit the present application. Various modifications and variations are possible for those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present application shall be included within the scope of protection of the present application.

Claims

1. A biological sample cryopreservation device, characterized by: The invention comprises a heat preservation part (10), wherein the heat preservation part (10) has a low-temperature storage cavity, wherein multiple groups of frozen storage groups for storing biological samples and a rotating assembly for driving the multiple groups of frozen storage groups to perform intermittent rotational motion are placed in the low-temperature storage cavity; a transition part (20) has a transition area located around the top access opening of the heat preservation part (10), wherein a driving assembly for driving the rotating assembly is arranged in the transition area; a heat preservation access cavity (30) has a access cavity, wherein the bottom opening of the access cavity is connected to the top of the low-temperature storage cavity; the bottom of the lifting part (40) is arranged at the top of the heat preservation tank body (101) located in the transition area, and the top of the lifting part (40) is provided with a lifting assembly that can move horizontally linearly, and the lifting part of the lifting assembly can sequentially pass through the heat preservation access cavity (30) and the transition part (20) and extend into the heat preservation part (10); The heat-insulating tank (101) further comprises a first liquid level sensor (108), a liquid inlet pipe (109), a first temperature sensor (110), and a second liquid level sensor (111), wherein the first liquid level sensor (108) and the liquid inlet pipe (109) are both located near the lower surface of the turntable (106), the first temperature sensor (110) is located on the outer peripheral wall of the turntable (106), and the second liquid level sensor (111) is located inside the heat-insulating tank (101). The top portion of the heat preservation tank body (101); the liquid replenishing valve (112), the liquid inlet valve (113) and the exhaust valve (114) are all located on the outer peripheral wall of the heat preservation tank body (101); the liquid inlet valve (113) is connected to the liquid replenishing valve (112); the liquid replenishing valve (112) is connected to the liquid inlet pipe (109); the exhaust valve (114) is connected between the liquid replenishing valve (112) and the liquid inlet valve (113); and the exhaust valve (114) is connected to the vertical channel (311); and the second temperature sensor is located in the cylinder body (301).

2. The biological sample cryopreservation device according to claim 1, characterized in that: The heat preservation part (10) comprises a heat preservation tank body (101), wherein the heat preservation tank body (101) has a low-temperature storage cavity, a heat preservation tank body sampling port (102) is provided at the top of the low-temperature storage cavity, and a heat preservation plug cover B (103) and a heat preservation plug cover A (104) are placed at the heat preservation tank body sampling port (102); The rotating assembly comprises a turntable (106) and a rotating shaft (115), wherein the turntable (106) is arranged at the lower section of the rotating shaft (115), a refrigerated storage group is placed on the turntable (106), the bottom of the rotating shaft (115) is rotatably arranged at the center position of the heat preservation tank base (105) at the bottom of the heat preservation tank (101), and the top of the rotating shaft (115) is rotatably arranged at the top of the heat preservation tank (101).

3. The biological sample cryopreservation device according to claim 2, characterized in that: The contact surface between the thermal insulation plug body cover B (103) and the thermal insulation plug body cover A (104) is a stepped surface.

4. The biological sample cryopreservation device according to claim 2, characterized in that: The frozen storage group includes a liquid nitrogen freezing rack (107), and the area of ​​the heat-insulating tank body (101) located above the turntable (106) forms a plurality of mutually independent freezing placement areas, each freezing placement area is provided with a plurality of the liquid nitrogen freezing racks (107), and a plurality of freezing boxes are vertically arranged on the liquid nitrogen freezing rack (107).

5. The biological sample cryopreservation device according to claim 4, characterized in that: The driving assembly includes a speed reducer (201), a chain (202), a driven wheel (203), a motor (204) and a driving wheel (205). The reducer (201) and the motor (204) are both arranged at the top of the heat-insulating tank body (101) located in the transition area, the input end of the reducer (201) is connected to the output end of the motor (204), the output end of the reducer (201) is provided with a driving wheel (205), the top of the rotating shaft (115) extending out of the heat-insulating tank body (101) is provided with a driven wheel (203), and the driven wheel (203) and the driving wheel (205) are linked to each other by a chain (202).

6. The biological sample cryopreservation device according to claim 5, characterized in that: The heat-insulating taking-and-putting part (30) comprises a cylinder (301), the cylinder (301) has a taking-and-putting cavity, a cylinder top opening (303) is provided at the top of the cylinder (301), a plug cover (304) is placed on the cylinder top opening (303), a plug cover strip slit (305) is provided at the middle position of the plug cover (304), a spray assembly is provided on the inner wall surface of the upper section of the taking-and-putting cavity, and a taking-and-putting window assembly is provided at the middle and lower sections of the taking-and-putting cavity. The spray assembly comprises a nozzle (309), an annular channel (310) and a vertical channel (311); a plurality of output holes are provided in an annular manner on the lower side of the annular channel (310); each output hole is provided with a nozzle (309); an input hole is provided on the upper side of the annular channel (310); the input hole is communicated with the output end of the vertical channel (311); The access window assembly includes an access door (302), a movable door (306), a strip-shaped observation hole (307), a limiting guide strip (308), a vertical slide groove (312), a vertical slider (313) and a locking bolt (314); a access window is provided in the middle and lower sections of the access cavity; a connecting piece (315) is provided on the outer peripheral wall surface of the cylinder (301) above the access window; and limiting guide strips (308) are provided on the left and right sides of the access window; The take-and-put window is provided with a take-and-put door (302) that can be flipped open and closed, and the movable door (306) is located between the cylinder (301) and the take-and-put door (302). The left and right side edges of the movable door (306) respectively correspond to the inner side surface of the limiting guide strip (308) for sliding fit, and a vertical sliding groove (312) is vertically opened at the middle position of the movable door (306). The vertical sliding groove (312) is slidably sleeved with the connecting piece (315). The sliding groove part of the vertical sliding groove (312) located above the connecting piece (315) is slidably sleeved with a vertical sliding block (313) through a locking bolt (314), and the vertical movement of the vertical sliding block (313) can be locked by the locking bolt (314). The movable door (306) is provided with a strip-shaped observation hole (307) at a position below the vertical sliding groove (312).

7. The biological sample cryopreservation device according to claim 6, characterized in that: The lifting part (40) includes a lifting support frame (401), the heat-insulating tank body (101) of the lifting support frame (401) is located at the top of the transition area, and a lifting component is provided on the top of the lifting support frame (401), and the lifting component includes an aluminum plate, a slide, a guide rail, a strip aluminum profile, a lifting frame, a side slider, a rotating wheel, a lifting motor, a chain rope (402), a hook, a wire trough support frame, a tank chain wire trough, a ring chain and a driving motor. The aluminum plate is fixedly arranged on the top of the lifting support frame (401), the upper surface of the aluminum plate is provided with a slide groove, a guide rail is slidingly arranged in the slide groove, the upper surface of the guide rail is provided with a strip aluminum profile, one end of the strip aluminum profile is provided with a lifting frame, a rotating wheel is provided in the lifting frame through a first rotating shaft, one end of the first rotating shaft is connected to the lifting motor, one end of the chain rope (402) is provided on the rotating wheel, and the other end of the chain rope (402) is provided with a hook, A wire trough support frame is provided on the upper surface of the aluminum plate on one side of the slide trough, the tank chain wire trough is provided on the upper surface of the wire trough support frame, a ring chain is provided in the tank chain wire trough, a sprocket is provided on the upper surface of the wire trough support frame away from the tank chain wire trough for rotation via a second rotating shaft, the part of the chain extending out of the tank chain wire trough is linked to the sprocket, and the drive motor is connected to one end of the second rotating shaft.

8. The biological sample cryopreservation device according to claim 7, characterized in that: The storage device also includes a laser locator, which is arranged on one side of the lifting frame.

9. The biological sample cryopreservation device according to claim 8, characterized in that: A stepped rack (116) is also provided on one side of the bottom of the heat-insulating tank body (101), and an endoscope (501) is also placed on the top of the heat-insulating tank body (101) within the transition area.

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