Sample pretreatment device for high-throughput mass spectrometer detection

Abstract

The utility model belongs to the technical field of mass spectrometer detection, and particularly relates to a sample pretreatment device for high-flux mass spectrometer detection, which adopts the following scheme that the sample pretreatment device comprises a box body, a cover plate is arranged on the box body in a sealing connection manner, a porous panel is arranged in the box body and is supported by a stand column, a plurality of pore passages are distributed on the cover plate in an array manner, and the porous panel is arranged in the box body. Pore channels corresponding to the pore channels one to one are formed in the porous panel and used for supporting and placing the collecting container, and adapters with flow control valves are inserted into the pore channels. The multi-hole panel of the collecting device can meet the collecting requirements of liquid of different specifications, and the using flexibility of the device is improved. The device is simple in structural design, the adopted materials are convenient to clean, the operability is good, one-time high-throughput continuous treatment of samples can be realized, and the device is convenient to popularize and use.

Description

TECHNICAL FIELD

[0001] The utility model relates to mass spectrometer detection technical field especially relates to a kind of sample pretreatment device for high-throughput mass spectrometer detection. BACKGROUND

[0002] Solid phase extraction is a kind of sample pretreatment technology, mainly through the selective adsorption and elution characteristics of solid phase extraction column, the analyte to be measured is separated from complex sample, so as to improve the detection sensitivity, achieve the purpose of extraction, separation and concentration. Solid phase extraction steps are generally divided into four steps of pretreatment activation, sample loading, elution and elution. First, the extraction column is pretreated and activated with an appropriate solvent to remove the impurities on the column that affect the extraction performance. Then the sample is added to the column, the flow rate is controlled, and the adsorption is fully adsorbed. The weakly adhering impurities are selectively removed by an appropriate cleaning solvent to retain the target analyte. Finally, the target analyte is eluted by adding an elution solvent for subsequent detection and analysis.

[0003] The existing extraction device has the following problems:

[0004] 1. The number of samples processed at one time is small, which cannot meet the detection of large-throughput samples, and the detection efficiency is low.

[0005] 2. The sample loading volume is limited (less than 10 mL). When the sample loading volume is large, multiple step loading is required, which is complicated.

[0006] 3. The volume of the eluent collection tube is fixed, which cannot meet the elution requirements of large-capacity samples.

[0007] 4. The liquid flow rate cannot be controlled individually after the extraction column is loaded.

[0008] 5. Open extraction, waste liquid generated during the experiment poses a threat to the health of the experimenters. UTILITY MODEL CONTENT

[0009] The utility model aims at the defects of prior art, and provides a sample pretreatment device for high-throughput mass spectrometer detection to solve the problems raised in the background art.

[0010] A sample pretreatment device for high-throughput mass spectrometer detection, comprising a box body, a sealingly connected cover plate is provided on the box body, a multi-hole panel supported by a stand is arranged inside the box body, a plurality of holes are arrayed on the cover plate, a hole corresponding to each hole is formed on the multi-hole panel for supporting and placing a collection container, an adapter with a flow control valve is inserted at the hole, an extraction column is installed at the top end of the adapter, and a sample liquid storage container is installed at the top of the extraction column.

[0011] By adopting the technical scheme, by increasing the sample solution storage container, one-time large-volume sample loading can be realized, the volume limitation of the extraction column can be overcome, continuous operation can be realized, and continuous and stable operation in the operation process can be ensured.

[0012] The cover plate is further provided with a pressure gauge and a connecting pipe, and the connecting pipe is connected with a vacuum pump at one end.

[0013] By adopting the technical scheme, negative pressure is realized in the box body.

[0014] The upper end of the sample solution storage container is a wide-angle opening, and the lower end is a circular truncated cone-shaped liquid outlet.

[0015] By adopting the technical scheme, it is convenient to add the sample loading liquid.

[0016] The upper end of the adapter is provided in a circular truncated cone structure, and different inner diameter ring-shaped sealing rings are arranged on the upper end, so that the upper end of the adapter can be fixedly connected with different extraction columns.

[0017] By adopting the technical scheme, the extraction column can be conveniently fixed.

[0018] The bottom of the adapter is detachably provided with a dropper for guiding liquid to drop into the collection container.

[0019] By adopting the technical scheme, the collection container can collect liquid.

[0020] The sample pretreatment device for high-throughput mass spectrometer detection has the following beneficial effects:

[0021] 1. By increasing the sample solution storage container, one-time large-volume sample loading can be realized, the volume limitation of the extraction column can be overcome, continuous operation can be realized, and continuous and stable operation in the operation process can be ensured.

[0022] 2. The multi-channel box body structure is adopted, 70-100 large-throughput samples can be processed at the same time, and the sample pretreatment efficiency is improved.

[0023] 3. The adapter structure is adopted, and the flow rate of a single extraction column is controlled by a switch, so that the detection method suitable for different sample loading flow rates is adapted.

[0024] 4. The closed box body structure is adopted, environmental pollution is reduced, and the experimental operator is friendly.

[0025] 5. The multi-hole panel of the collection device can adapt to different specifications of liquid collection requirements, and increase the use flexibility of the device. The device has simple structure, the material is convenient to clean, and the operation is good, so that one-time high-throughput sample continuous processing can be realized, and the device is convenient to use. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 The utility model discloses a high throughput mass spectrometer detection sample pretreatment device structure schematic diagram is provided for the utility model,

[0027] Figure 2 The utility model discloses a high throughput mass spectrometer detection sample pretreatment device's decomposition schematic diagram is provided for the utility model,

[0028] Figure 3 The utility model discloses a high throughput mass spectrometer detection sample pretreatment device cover plate's component distribution diagram is provided for the utility model,

[0029] Figure 4 The utility model discloses a high throughput mass spectrometer detection sample pretreatment device's adapter, extraction column and sample solution storage container structure schematic diagram is provided for the utility model.

[0030] In the drawing: 1, box body;101, stand column;2, cover plate;201, pore channel;3, multi -hole panel;4, collection container;5, adapter;501, flow control valve;6, extraction column;7, sample solution storage container;8, pressure gauge;9, dropper;10, connecting pipe;11, vacuum pump. Specific implementation

[0031] The preferable embodiments of the utility model are described in detail below in combination with the drawings, so that the advantages and features of the utility model can be more easily understood by the person skilled in the art, and the protection scope of the utility model can be more clearly and explicitly defined.

[0032] Reference Figures 1-4 A kind of sample pretreatment device for high throughput mass spectrometer detection, including box body 1, box body 1 is equipped with the cover plate 2 of sealed connection, box body 1 inside is provided with the multi -hole panel 3 supported by stand column 101, cover plate 2 is arrayed with multiple pore channels 201, the hole channel correspondingly set with multiple pore channels 201 is opened in multi -hole panel 3, for supporting to place collection container 4, adapter 5 with flow control valve 501 is inserted and is equipped with in pore channel 201, adapter 5 top end portion is installed with extraction column 6, extraction column 6 top is installed with sample solution storage container 7.

[0033] Among them, sample solution storage container 7 adopts PP material, the wide-angle opening is set in the upper end portion of sample solution storage container 7, its lower end portion is circular truncated cone liquid outlet setting, for the convenience of adding sample liquid, can be adapted to different kinds, specification extraction column 6, (need to explain Figure 1The lower end of the extraction column 6 is connected with the upper end of the adapter, the adapter 5 is made of PTFE material, the upper end of the adapter 5 is provided with a circular table structure, and different inner diameter ring-shaped sealing rings are arranged on the upper end of the adapter 5, so that the upper end of the adapter 5 can be fixedly connected with different extraction columns 6, the different inner diameter ring-shaped sealing rings are arranged, the fixed connection of different extraction columns 6 is facilitated, the flow control valve 501 is arranged in the middle of the adapter 5, and the flow rate is adjusted; wherein the box body 1 is made of PVC material, the size of the box body 1 is (30*35cm), the cover plate 2 is further provided with a pressure gauge 8 and a connecting pipe 10, one end of the connecting pipe 10 is connected with a vacuum pump 11, the cover plate 2 is provided with a sealing ring around, and the top is provided with a plurality of holes 201 for fixedly connecting the adapter 5; the cover plate 2 is provided with a pressure gauge 8 and a connecting pipe 10, and is used for connecting the vacuum pump 11; the bottom plate of the box body 1 is provided with four columns 101 for supporting and fixing the multi-hole panel 3, and can be used for placing different specifications of the collection container 4. When the sample pretreatment extraction operation is carried out, first, the lower end of the adapter 5 is inserted into the corresponding hole 201 of the cover plate 2 of the box body 1 and is tightly fixed, the flow control valve 501 is closed according to the sample quantity design, and the unnecessary adapter 5 is closed; then the sample storage container 7 is placed on the upper end of the extraction column 6 and is tightly fixed, then the lower end of the extraction column 6 is inserted into the upper end of the tapered port of the adapter 5, and the liquid flow rate is adjusted as required; according to the experimental requirements, the multi-hole panel 3 with different container hole diameters such as 15mL and 50mL can be selected, the dropper 9 is detachably arranged at the bottom of the adapter 5, and is used for guiding the liquid to drop into the collection container 4, so that the collection container 4 can collect the liquid, the box body 1 can realize internal negative pressure through the vacuum pump 11, the pressure of the box body 1 is detected by the pressure gauge 8, and the extraction progress is accelerated. The device can realize one-time large-throughput sample loading treatment, meet the one-time large-volume sample loading for continuous operation, flexibly select different types of collection devices, meet the eluent collection of large-volume samples, the flow rate of each extraction column 6 can be individually controlled through an independent valve, and different detection requirements are met; the closed treatment device reduces environmental pollution and is friendly to experimental operators. The device can significantly improve the efficiency of sample pretreatment work and is suitable for sample pretreatment requirements of different detection methods.

[0034] Example 1: Determination of sucralose (sucrose) in food: the sample purification process needs to rely on the device throughout the process: before using the solid phase extraction column 6, activate it with 4mL of methanol and 4mL of water in turn, keep the column wet. Move all the sample extraction supernatant into the activated solid phase extraction column 6, control the liquid flow rate not to exceed 1 drop / s, add 1mL of water when the liquid surface on the column is about 2mm, continue to maintain the liquid flow rate at 1 drop / s, after the liquid in the column is completely discharged, elute with 3mL of methanol, collect all the eluate, evaporate to dryness on a boiling water bath, dissolve the residue with 1.00mL of water + acetonitrile solution (89+11) (if the solution has turbidity, it can be moved into a centrifuge tube, centrifuged for 5min, filtered through a 0.45μm hydrophobic microporous filter, and the filtrate is the prepared sample solution for testing.

[0035] The above-described embodiments only express several embodiments of the present application, and the description is more specific and detailed, but it should not be understood as limiting the scope of the application. It should be noted that for ordinary skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which are all within the scope of protection of the present application.

Claims

1. A sample pretreatment device for high-throughput mass spectrometer detection, characterized in that: The invention comprises a box body (1), wherein a sealingly connected cover plate (2) is provided on the box body (1), a porous panel (3) supported by a column (101) is provided inside the box body (1), a plurality of channels (201) are distributed in an array on the cover plate (2), channels corresponding to the plurality of channels (201) are provided on the porous panel (3) for supporting and placing a collection container (4), an adapter (5) with a flow control valve (501) is inserted at the channel (201), an extraction column (6) is installed at the top end of the adapter (5), and a sample liquid storage container (7) is installed at the top end of the extraction column (6).

2. The sample pretreatment device for high-throughput mass spectrometer detection according to claim 1, characterized in that: A pressure gauge (8) and a connecting pipe (10) are also installed on the cover plate (2), and one end of the connecting pipe (10) is connected to a vacuum pump (11).

3. The sample pretreatment device for high-throughput mass spectrometer detection according to claim 2, characterized in that: The upper end of the sample liquid storage container (7) is a wide-angle opening, and the lower end thereof is a truncated cone-shaped liquid outlet.

4. The sample pretreatment device for high-throughput mass spectrometer detection according to claim 3, characterized in that: The upper end of the adapter (5) is a truncated cone-shaped structure, and annular sealing rings with different inner diameters are provided thereon, so that the upper end of the adapter (5) can be fixedly connected to different extraction columns (6).

5. The sample pretreatment device for high-throughput mass spectrometer detection according to claim 4, characterized in that: A dropper (9) is detachably mounted on the bottom of the adapter (5) for guiding the liquid to drip into the collection container (4).

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