Preparation equipment of double-C-line fluorescent immune layer detection reagent
By installing partitions and lifting frames in the soaking tank, multiple nitrocellulose membranes can be soaked in the same tank at different times, which solves the problem of difficult control of the soaking time of nitrocellulose membranes, improves the efficiency of use and saves the amount of buffer solution used.
Patent Information
- Application Number
- CN202520308237.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-25
- Publication Date
- 2025-11-28
- Estimated Expiration
- 2035-02-25
AI Technical Summary
In existing nitrocellulose membrane treatment processes, it is difficult to achieve comparative immersion at different time periods in the same immersion tank, and the buffer solution has low utilization efficiency, resulting in waste and increased costs.
An immersion tank with multiple partitions was designed, which divides the immersion tank into multiple immersion compartments. The immersion time is controlled by electric push rods and lifting frames. Combined with water pumps and liquid injection pipeline systems, multiple nitrocellulose membranes can be immersed in different time periods.
This technology enables nitrocellulose membrane immersion at different time periods in the same environment, reducing buffer usage, improving efficiency, and lowering economic costs.
Smart Images
Figure CN223611510U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to double C line fluorescence immunoassay test reagent preparation technical field, concretely is a kind of double C line fluorescence immunoassay test reagent's preparation equipment. BACKGROUND
[0002] Double C line fluorescence immunoassay test reagent is a kind of technical means commonly used in medical detection, it is based on the principle of immunochromatography, and specific binding occurs between fluorescently labeled antibody and the substance to be measured, so as to produce fluorescence signal to realize detection.
[0003] The preparation process of double C line fluorescence immunoassay test reagent generally includes antibody selection and labeling, nitrocellulose membrane treatment, antibody coating, sample pad treatment, binding pad treatment, assembly, drying and quality control, and the specific preparation process in each step needs to be adjusted according to the different needs of operators.
[0004] In the existing nitrocellulose membrane treatment, the nitrocellulose membrane needs to be put into a buffer solution with a certain concentration, such as phosphate buffer solution, and soaked for ten to thirty minutes to remove impurities on the surface of the nitrocellulose membrane and make it fully wet. However, when soaking, a large number of nitrocellulose membranes are usually put into the soaking box. After being used once, new buffer solution needs to be replaced. When the user has specific requirements for the soaking time, it is difficult to form a plurality of comparison nitrocellulose membranes with different soaking times inside the same soaking box. Moreover, a large number of nitrocellulose membranes are put into the soaking box, and the nitrocellulose membranes are stacked, so that part of the buffer solution is wasted, and the use efficiency is reduced. UTILITY MODEL CONTENTS
[0005] The utility model aims to provide a kind of double C line fluorescence immunoassay test reagent's preparation equipment to solve the problems raised in the above background art.
[0006] To achieve the above object, the utility model provides the following technical scheme: a kind of double C line fluorescence immunoassay test reagent's preparation equipment, including soaking tank, the inside of the soaking tank is installed with multiple partition plates, multiple the partition plates divide the cavity inside the soaking tank into multiple soaking compartments, the inside bottom end of the soaking compartment is installed with two electric push rods, the inside of the soaking compartment is slidably connected with lifting frame, the inside of the lifting frame is installed with mounting frame, the inside of the mounting frame is paved with nitrocellulose membrane, the surface of the mounting frame is detachably installed with sliding glass, the surface of the sliding glass is equipped with multiple liquid passing holes, the right side of the soaking tank is provided with liquid storage bin, the upper end of the liquid storage bin is sequentially communicated with multiple soaking compartments by multiple liquid injection pipelines, the inside of the liquid injection pipeline is provided with water pump.
[0007] Preferably, the outside left side of the soaking box is provided with a temporary storage box, the temporary storage box is communicated with the inside bottom end of a plurality of soaking compartments in sequence through a plurality of connecting pipes, a water pump is installed in the connecting pipe, an output pipeline is installed on the outside of the temporary storage box, and the output pipeline is connected with an external buffer solution storage device.
[0008] Preferably, a plurality of reinforcing blocks are installed at the front and back ends of the liquid storage bin and the temporary storage bin, and the other ends of the reinforcing blocks are connected with the surface of the soaking box.
[0009] Preferably, two clamping grooves are formed in the bottom of the lifting frame, and the positions and sizes of the two clamping grooves are matched with the two electric push rods.
[0010] Preferably, a sliding groove is formed in each of the two shorter side walls of the soaking compartment, and a sliding block matched with the sliding groove is installed on each of the two shorter side edges of the lifting frame.
[0011] Preferably, a clamping and overturning frame is rotatably connected in the inside of the mounting frame, a through groove is formed in the side wall of the mounting frame, the sliding glass is slidably connected with the through groove, and the bottom end of the sliding glass is in sliding contact with the upper end of the clamping and overturning frame.
[0012] Compared with the prior art, the utility model has the advantages that:
[0013] 1. The preparation equipment of the double C-line fluorescent immunochromatographic assay reagent is characterized in that a plurality of nitrocellulose membranes are stacked in the inside of the mounting frame, transparent glass is installed, the mounting frame is placed in the inside of the lifting frame, the lifting frame is slowly placed in the inside of the soaking compartment, buffer solution is injected into the inside of the soaking compartment through the liquid injection pipeline, until the buffer solution completely covers the lifting frame, the buffer solution enters the inside of the mounting frame through a plurality of liquid passing holes and covers the nitrocellulose membrane, different start times are set for the electric push rods in different soaking compartments, the lifting frame can be pushed to the outside of the soaking box in the same environment at different times, the mounting frame can be taken out by artificial at this time, and the demand of a user for nitrocellulose membranes with different soaking times in the same environment can be met.
[0014] 2. The preparation equipment of the double C-line fluorescent immunochromatographic assay reagent is characterized in that the inside of the soaking box is divided into a plurality of soaking compartments by the partition plate, one lifting frame is placed in each soaking compartment, when the buffer solution is injected into the inside of the soaking compartment, since the lifting frame itself occupies a considerable space in the soaking compartment, although the buffer solution finally completely covers the lifting frame, the total volume of the internal liquid in the soaking compartment is still small, the use amount of the buffer solution can be reduced while the nitrocellulose membrane is completely soaked, and the economic cost of a user is saved. BRIEF DESCRIPTION OF DRAWINGS
[0015] Fig. 1 It is the structure schematic view of the whole structure of the utility model;
[0016] Fig. 2 It is the structure schematic view of the lifting frame and the mounting frame of the utility model;
[0017] Fig. 3 It is the structure schematic view inside the soaking box of the utility model;
[0018] Fig. 4 It is the structure schematic view inside the mounting frame of the utility model.
[0019] In the drawing: 1, soaking box;2, liquid storage bin;3, temporary storage box;4, output pipeline;5, connecting pipe;6, reinforcing block;7, lifting frame;8, clamping groove;9, sliding block;10, mounting frame;11, sliding glass;12, liquid passing hole;13, electric push rod;14, partition plate;15, soaking compartment;16, sliding groove;17, clamping and overturning frame;18, through groove;19, liquid injection pipeline. DETAILED DESCRIPTION
[0020] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.
[0021] In the description of the utility model, it should be explained that the directions or position relations indicated by the terms "upper", "lower", "inner", "outer", "front end", "rear end", "two ends", "one end", "the other end" and the like are the directions or position relations shown in the drawings, which are only for the convenience of describing the utility model and simplifying the description, and cannot be understood as indicating or implying that the devices or elements must have a particular direction, be constructed and operated in a particular direction, and therefore cannot be understood as limiting the utility model. In addition, the terms "first" and "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.
[0022] As Figs. 1 to 4As shown, the preparation equipment of the double C-line fluorescent immunochromatographic test reagent of the embodiment includes a soaking box 1, a plurality of partition plates 14 are installed inside the soaking box 1, the plurality of partition plates 14 are arranged at equal intervals, the plurality of partition plates 14 divide the internal cavity of the soaking box 1 into a plurality of soaking compartments 15, two electric push rods 13 are installed at the bottom of the inside of the soaking compartment 15, a plurality of electric push rods 13 are connected to an external control platform, the lifting movement of the electric push rod 13 is controlled through the external control platform, the electric push rod 13 can move up and down in the vertical direction, a lifting frame 7 is slidably connected to the inside of the soaking compartment 15, the bottom of the lifting frame 7 is connected to the top of the electric push rod 13, an installation frame 10 is installed in the inside of the lifting frame 7, the size of the installation frame 10 is adapted to the size of the internal space of the lifting frame 7, so that the installation frame 10 can be installed in the inside of the lifting frame 7, nitrocellulose membranes are laid in the inside of the installation frame 10, a sliding glass 11 is detachably installed on the surface of the installation frame 10, a plurality of liquid passing holes 12 are formed in the surface of the sliding glass 11, a liquid storage bin 2 is arranged on the right side of the soaking box 1, the upper end of the liquid storage bin 2 is communicated with a plurality of soaking compartments 15 in sequence through a plurality of liquid injection pipelines 19, a water pump is arranged in the inside of the liquid injection pipeline 19, the buffer solution in the inside of the liquid storage bin 2 can be pumped into the inside of the liquid injection pipeline 19 through the water pump, and then into the inside of the soaking compartment 15, different start times are set for the electric push rods 13 in different soaking compartments 15, so that the lifting frame 7 can be pushed out of the soaking box 1 in sequence at different times in the same environment, at this time, the installation frame 10 can be taken out manually, which can meet the needs of users for nitrocellulose membranes with different soaking times in the same environment.
[0023] Specifically, a temporary storage box 3 is arranged on the left side of the outside of the soaking box 1, the temporary storage box 3 is communicated with the bottom of the inside of a plurality of soaking compartments 15 in sequence through a plurality of connecting pipes 5, a water pump is installed in the inside of the connecting pipe 5, an output pipeline 4 is installed on the outside of the temporary storage box 3, the output pipeline 4 is connected to an external buffer solution storage device, after each time of soaking the nitrocellulose membrane, the used buffer solution needs to be pumped into the inside of the temporary storage box 3 through the connecting pipe 5, when the temporary storage box 3 is about to be filled, the buffer solution is discharged into the inside of the external storage device through the output pipeline 4, it should be noted that the connection of the connecting pipe 5 is provided with a valve control switch.
[0024] Further, a plurality of reinforcing blocks 6 are installed at the front and back ends of the liquid storage bin 2 and the temporary storage box 3, the other end of the reinforcing block 6 is connected to the surface of the soaking box 1, the reinforcing blocks 6 are detachably connected, which enhances the connection relationship between the liquid storage bin 2 and the temporary storage box 3 and the soaking box 1, and enhances the stability.
[0025] Further, the bottom of the lifting frame 7 is provided with two clamping grooves 8, the positions and sizes of the two clamping grooves 8 are matched with the two electric push rods 13, the two shorter side walls of the soaking compartment 15 are provided with a sliding groove 16, and the two shorter side edges of the lifting frame 7 are provided with a sliding block 9 matched with the sliding groove 16, so that the electric push rod 13 can be better matched with the lifting frame 7, and the lifting frame 7 is slidably connected to the inside of the soaking compartment 15 through the sliding block 9 and the sliding groove 16.
[0026] Further, the inside of the mounting frame 10 is rotatably connected with a clamping and overturning frame 17, the side wall of the mounting frame 10 is provided with a through groove 18, and the sliding glass 11 is slidably connected with the through groove 18, and the bottom end of the sliding glass 11 is in sliding contact with the upper end of the clamping and overturning frame 17, so that after the nitrocellulose membrane is placed in the inside of the mounting frame 10, the nitrocellulose membrane can be clamped by rotating the clamping and overturning frame 17, and then the sliding glass 11 is moved above the clamping and overturning frame 17 to limit the clamping and overturning frame 17, so that the position of the nitrocellulose membrane is limited, thereby ensuring that the position of the nitrocellulose membrane will not change when the nitrocellulose membrane is soaked in the buffer solution.
[0027] The use method of the embodiment is that a plurality of nitrocellulose membranes are stacked in the inside of the mounting frame 10, and the transparent glass is installed, the mounting frame 10 is placed in the inside of the lifting frame 7, and then the lifting frame 7 is slowly placed in the inside of the soaking compartment 15, the buffer solution is injected into the inside of the soaking compartment 15 through the liquid injection pipe 19 until the buffer solution completely covers the lifting frame 7, the buffer solution enters the inside of the mounting frame 10 through the plurality of liquid passing holes 12 and covers the nitrocellulose membrane, different start times are set for the electric push rods 13 in different soaking compartments 15, the lifting frame 7 is sequentially pushed out of the soaking tank 1 in the same environment, and then the mounting frame 10 is taken out by artificial, so that the needs of users for nitrocellulose membranes with different soaking times in the same environment can be met.
[0028] Finally, it should be noted that: the above only describes the preferred embodiments of the present application, and is not used to limit the present application, although the present application has been described in detail with reference to the foregoing embodiments, and for those skilled in the art, the technical solutions recorded in the foregoing embodiments can be modified, or some technical features can be replaced. Any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application shall be included in the protection scope of the present application.
Claims
1. A preparation device of double C-line fluorescent immunochromatographic test reagent, comprising a soaking box (1), characterized in that: The inside of the soaking box (1) is internally provided with a plurality of partition plates (14), which divide the internal cavity of the soaking box (1) into a plurality of soaking compartments (15), the inside of the soaking compartment (15) is internally provided with two electric push rods (13) at the bottom end, the inside of the soaking compartment (15) is slidably connected with a lifting frame (7), the inside of the lifting frame (7) is internally provided with a mounting frame (10), the inside of the mounting frame (10) is paved with nitrocellulose membrane, the surface of the mounting frame (10) is detachably provided with a sliding glass (11), the surface of the sliding glass (11) is provided with a plurality of liquid passing holes (12), the right side of the soaking box (1) is provided with a liquid storage bin (2), the upper end of the liquid storage bin (2) is sequentially communicated with a plurality of soaking compartments (15) through a plurality of liquid injection pipelines (19), and the inside of the liquid injection pipeline (19) is provided with a water pump.
2. The apparatus for preparing a double C-line fluorescent immunoassay test reagent according to claim 1, characterized by: The left side of the outside of the soaking box (1) is provided with a temporary storage box (3), the temporary storage box (3) is sequentially communicated with the inside bottom end of a plurality of soaking compartments (15) through a plurality of connecting pipes (5), the inside of the connecting pipe (5) is internally provided with a water pump, the outside of the temporary storage box (3) is provided with an output pipeline (4), and the output pipeline (4) is connected with an external buffer liquid storage device.
3. The apparatus for preparing a double C-line fluorescent immunoassay test reagent according to claim 1, characterized by: The front and rear ends of the liquid storage bin (2) and the temporary storage bin are both provided with a plurality of reinforcing blocks (6), and the other end of the reinforcing block (6) is connected with the surface of the soaking box (1).
4. The apparatus according to claim 1, wherein the apparatus is characterized by: The bottom of the lifting frame (7) is provided with two clamping grooves (8), and the positions and sizes of the two clamping grooves (8) are matched with the two electric push rods (13).
5. The apparatus according to claim 1, wherein the apparatus is characterized by: Both of the two shorter side walls of the soaking compartment (15) are provided with a sliding groove (16), and both of the two shorter side edges of the lifting frame (7) are provided with a sliding block (9) matched with the sliding groove (16).
6. The apparatus according to claim 1, wherein the apparatus is characterized by: The inside of the mounting frame (10) is rotatably connected with a clamping and overturning frame (17), the sidewall of the mounting frame (10) is provided with a through groove (18), the sliding glass (11) is slidably connected with the through groove (18), and the bottom end of the sliding glass (11) and the upper end of the clamping and overturning frame (17) are in sliding contact.