Integrated solid nanopore biological detector

Abstract

The utility model discloses an integrated solid-state nanopore biological detector, which relates to the technical field of biological detection and comprises a fluid pool shell, a solid-state nanopore silicon nitride chip and a PCB (printed circuit board), a sample injection hole is arranged on the fluid pool shell, the solid-state nanopore silicon nitride chip is arranged in the fluid pool shell, and the solid-state nanopore silicon nitride chip is arranged in the PCB. A silicon nitride window corresponding to the solid-state nanopore silicon nitride chip is formed in the fluid pool shell, a PCB (Printed Circuit Board) is fixed in the fluid pool shell, an RFID (Radio Frequency Identification Device) storage chip, a magnetic connector and silver electrodes are arranged on the PCB, and the silver electrodes are positioned on two sides of the silicon nitride window. According to the utility model, the RFID storage chip is arranged, so that the key information of the biological detector is stored and traced, and the problem of information management disorder in the prior art is solved; the RFID storage chip can store key information of the biological detector for a long time, and the integrity and traceability of the data are ensured.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the biological detection technical field, concretely relates to an integrated solid state nanopore biological detector. BACKGROUND

[0002] Solid state nanopore biological detection technology is a new biological analysis technology, which has been widely concerned in the field of biomedicine in recent years. Its core principle is to use a nanoscale hole (usually a solid state nanopore) as a detection channel, and to measure the current change caused by the passage of biological molecules (such as viruses, DNA, proteins, etc.) through the nanopore, so as to realize the rapid detection and analysis of biological molecules. This technology has the advantages of simple operation, fast detection speed, high sensitivity, and single molecule detection, and is widely used in the fields of virus detection, gene sequencing, protein analysis, disease diagnosis, etc.

[0003] Although solid state nanopore biological detection technology has many advantages, there are still some problems to be solved in the prior art, mainly in the following aspects: the existing solid state nanopore biological detector lacks effective information storage and traceability mechanism in the design and use process. For example, the key parameters of the nanopore (such as serial number, diameter, production time, via hole current, IV curve, etc.) usually need to be recorded manually, which is easy to lose or confuse. This confusion of information management makes it impossible to effectively trace and manage the key information of the biological detector in the production, transfer and use process, which brings great difficulty to the experimental repeatability, data verification and quality control; a large amount of key data will be generated during the use of the biological detector, such as detection time, operator information, detection result, etc. However, the existing equipment lacks effective data storage mechanism, which cannot save these information for a long time and reliably, which not only affects the integrity of the data, but also makes it difficult to trace and verify the detection results in subsequent research, and cannot meet the long-term storage and traceability requirements of the key information of the biological detector. UTILITY MODEL CONTENTS

[0004] In view of the problems existing in the prior art, the utility model provides an integrated solid state nanopore biological detector, which uses RFID (radio frequency identification) technology on the solid state nanopore biological detector to realize the storage and traceability of the key information of the solid state nanopore biological detector, and solves the problems of information management confusion and data storage deficiency in the prior art.

[0005] The utility model discloses a technical problem that it adopts the scheme of solving is: A kind of integrated solid-state nanopore biological detector, including fluid cell shell, solid-state nanopore silicon nitride chip and PCB board, sample injection hole is opened on fluid cell shell, solid-state nanopore silicon nitride chip is placed in fluid cell shell, corresponding silicon nitride window of solid-state nanopore silicon nitride chip is opened on fluid cell shell, PCB board is fixed in fluid cell shell, RFID storage chip, magnetic attraction connector and silver electrode are arranged on PCB board, and silver electrode is located the both sides of silicon nitride window.

[0006] Further, the fluid cell shell is composed of an upper cover and a base, threaded holes are formed in corresponding positions of the upper cover and the base, and the upper cover and the base are spliced by screws to form a complete fluid cell shell.

[0007] Further, the RFID storage chip is used for storing key information of the biological detector.

[0008] Further, the electrode is a silver electrode.

[0009] Further, the fluid cell shell has antibacterial and anti-static properties.

[0010] Compared with the prior art, the utility model has the beneficial effects that:

[0011] Strong information tracing capability: through the RFID storage chip, the storage and tracing of key information of the biological detector are realized, and the problem of information management confusion in the prior art is solved.

[0012] Reliable data storage: the RFID storage chip can long-term store the key information of the biological detector, ensuring the integrity and traceability of data.

[0013] Simple operation: the device has simple structure and is easy to operate, and is suitable for various biological detection scenes.

[0014] High detection efficiency: the detection liquid is driven to pass through the nanopore by electric field force, improving the detection efficiency and sensitivity. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 is one of the three-dimensional structure schematic diagrams of the utility model;

[0016] Figure 2 is the second three-dimensional structure schematic diagram of the utility model.

[0017] In the figure: 1, upper cover; 2, sample injection hole; 3, solid-state nanopore silicon nitride chip; 4, silver electrode; 5, base; 6, RFID storage chip; 7, PCB board; 8, silicon nitride window. DETAILED DESCRIPTION

[0018] The utility model is further illustrated below in combination with the drawings and embodiments.

[0019] Please refer to Figures 1-2 The utility model provides a kind of integrated solid-state nanopore biological detector technical scheme: Embodiments

[0020] According to Figure 1 And Figure 2 As shown in the figure, integrated solid-state nanopore biological detector is composed of fluid cell shell, solid-state nanopore silicon nitride chip 3, PCB board 7, silver electrode 4, RFID storage chip 6.The fluid cell is one of the core components of the detector, for containing sample detection liquid, usually made of plastic or metal material, with one or more sample injection holes for adding biological sample to be detected, the fluid cell is designed with channel inside, to ensure that sample liquid can uniformly flow through nanopore detection area.

[0021] Fluid cell shell is composed of upper cover 1 and base 5, threaded hole is formed on corresponding position of upper cover 1 and base 5, screw can be threadedly connected in threaded hole, and complete fluid cell shell is formed by screwing after upper cover 1 and base 5 are spliced.The material of fluid cell shell has antibacterial property, to prevent microbial contamination during detection, at the same time, the surface of shell is treated by anti-static, to reduce the interference of static to detection process, and improve the accuracy of detection.

[0022] Sample injection hole 2 is formed on upper cover 1, for adding sample detection liquid into fluid cell, solid-state nanopore silicon nitride chip 3 is placed in fluid cell shell, and solid-state nanopore chip is a key component of the detector, usually made of silicon nitride (Si3N4) or other insulating materials, nanoscale hole (nanopore) is processed on chip, the diameter is usually between 1-100 nanometers, the surface of nanopore is treated specially, to reduce non-specific adsorption and improve stability, sample detection liquid flows to the detection area of solid-state nanopore silicon nitride chip 3 through sample injection hole 2, and silicon nitride window 8 corresponding to solid-state nanopore silicon nitride chip 3 is also formed on upper cover 1.

[0023] Base 5 part is used for fixing PCB board 7, and RFID storage chip 6, magnetic joint and electrode are welded and installed on PCB board 7, electrode is usually made of silver, platinum or other conductive materials, and is located on both sides of nanopore, and silver electrode is taken as an example here, silver electrode 4 is located on both sides of silicon nitride window 8, electric field force is generated by applying 10mV~1000mV bias voltage to both ends of silver electrode 4, sample detection liquid passes through silicon nitride chip with solid-state nanopore under the driving action of electric field force, when pathogen passes through solid-state nanopore, the conductivity of nanopore is temporarily reduced due to its volume obstruction, and pulse waveform of current reduction is generated, so that the detection of pathogen is realized.

[0024] The RFID (electronic tag) storage chip welded on the PCB board 7 can be used to store key information of the biological detector, such as operator information, nanometer hole serial number, nanometer hole diameter, nanometer hole production time, nanometer hole via current, IV curve and the like, the information is written into the chip through an RFID reader-writer, and detection data is recorded in real time during detection, which is beneficial to trace the biological information, and through the RFID technology, the storage and trace of the key information of each nanometer hole biological detector are realized.

[0025] In specific use, the integrated solid-state nanometer hole biological detector has the following assembly steps: key information of the biological detector, such as a nanometer hole serial number and production time, is written into the RFID storage chip 6 through an RFID reader-writer, the solid-state nanometer hole silicon nitride chip 3 is arranged in the fluid pool, and the upper cover 1 and the base 5 are spliced through screws, so that the sample injection hole is aligned with the solid-state nanometer hole silicon nitride chip 3.

[0026] During detection, sample detection liquid is added into the fluid pool device through the sample injection hole 2, the sample liquid flows in the fluid pool and enters the detection area of the nanometer hole, then a bias voltage of 10 mV-1000 mV is applied between the silver electrodes 4 to generate an electric field force, the electric field force drives the biological molecules in the sample liquid to pass through the nanometer hole, when the pathogen passes through the nanometer hole, the conductivity of the nanometer hole will be temporarily reduced due to the volume blockage, resulting in a decrease in current, the detection circuit records the current change in real time to generate a current pulse signal detection report, and the RFID storage chip 6 records detection data, such as via current and IV curve information, in real time, the information in the RFID storage chip 6 is read through the RFID reader-writer, and the trace and management of the key information of the biological detector are realized.

[0027] The utility model can be applied to a solid-state nanometer hole puncher, a solid-state nanometer hole sequencer, a biological nanometer hole sequencer and other detectors that need to trace biological information.

[0028] The above description is only the preferred embodiment of the utility model, and does not limit the utility model, any modification, equivalent replacement and improvement made in the spirit and principle range of the utility model should be included in the protection range of the utility model.

Claims

1. An integrated solid-state nanopore biodetector, characterized in that: The device includes a fluid pool shell, a solid nanoporous silicon nitride chip (3) and a PCB board (7). The fluid pool shell has a sample injection hole (2). The solid nanoporous silicon nitride chip (3) is placed inside the fluid pool shell. A silicon nitride window (8) corresponding to the solid nanoporous silicon nitride chip (3) is opened on the fluid pool shell. The PCB board (7) is fixed inside the fluid pool shell. An RFID storage chip (6), a magnetic connector and electrodes are provided on the PCB board (7). The electrodes are located on both sides of the silicon nitride window (8).

2. The integrated solid-state nanopore biodetector according to claim 1, characterized in that: The fluid pool shell consists of two parts: a top cover (1) and a base (5). Threaded holes are provided at corresponding positions on the top cover (1) and the base (5). The top cover (1) and the base (5) are joined together by screws to form a complete fluid pool shell.

3. The integrated solid-state nanopore biodetector according to claim 1, characterized in that: The RFID storage chip (6) is used to store key information of the biodetector.

4. The integrated solid-state nanopore biodetector according to claim 1, characterized in that: The electrode is a silver electrode (4).

5. The integrated solid-state nanopore biodetector according to claim 1, characterized in that: The fluid pool shell has antibacterial and antistatic properties.

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