Large-scale cell culture device
By introducing a rotation and lifting mechanism into the cell culture device, the problem of microbial contamination during the removal of culture dishes was solved, and stable rotation of culture dishes and aseptic addition of nutrient solution were achieved, thereby improving the quality of cell culture.
Patent Information
- Application Number
- CN202423244668.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-27
- Publication Date
- 2026-01-30
- Estimated Expiration
- 2034-12-27
AI Technical Summary
During cell culture, the culture dish is easily contaminated by bacteria and airborne impurities when it is removed, which can affect the experimental results.
A large-scale cell culture device was designed, comprising a rotating mechanism and a lifting mechanism. The rotating mechanism achieves slow rotation of the culture dish through a sliding frame and a rotating disk to prevent centrifugal force from affecting the culture effect. The lifting mechanism enables the addition of nutrient solution without a large opening through a lifting block and a sliding plate, avoiding bacterial contamination.
It effectively prevents centrifugal force from affecting cell growth during the rotation of the culture dish, and reduces bacterial contamination by eliminating the need to add nutrient solution through a large opening, thus improving the cell culture effect.
Smart Images
Figure CN223852638U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to cell culture technical field, concretely is a kind of large-scale cell culture device. BACKGROUND
[0002] Cell culture is a method that cell is survived, grown, propagated and maintained main structure and function in vitro by simulating in-vivo environment, cell culture device can be in vitro cultured to cell / tissue, and is widely used in the research and production of scientific field such as microorganism, medicine, pharmacy, environmental protection, food, animal husbandry.
[0003] In the process of cell culture, the cell tissue state in the culture dish needs to be observed and recorded regularly or nutrient solution is added, when observing or adding nutrient solution, the culture dish needs to be taken out, at this time, the warehouse door needs to be opened, the taken-out culture dish is easy to be infected by miscellaneous bacteria, and impurities in the air can fall into the culture dish, causing pollution and affecting the experiment, therefore, a large-scale cell culture device needs to be improved to solve the above problems. UTILITY MODEL CONTENT
[0004] The utility model aims at providing a large-scale cell culture device to solve the problems in the above background.
[0005] To achieve the above object, the utility model provides the following technical scheme: a large-scale cell culture device, including cell culture box body, rotation mechanism is arranged in the cell culture box body, jacking mechanism is arranged in the cell culture box body.
[0006] Preferably, the rotation mechanism includes sliding frame, the sliding frame is slidably installed in the cell culture box body, the cell culture box body is fixedly installed with electric push rod one in the inside, the bottom of sliding frame is fixedly installed with motor, the output end of motor is fixedly installed with gear, the top of sliding frame is rotatably installed with rotating disc, the outside of rotating disc is fixedly installed with gear ring, the top of rotating disc is provided with culture dish, the culture dish is placed into the placing groove of rotating disc, after preventing the operator from entering the inside of cell culture box body, the vision is affected.
[0007] Preferably, the output end of electric push rod one is fixedly installed with sliding frame, the gear is engaged with gear ring, so that rotating disc slowly rotates, prevent the cell in the inside of culture dish from affecting cultivation effect because of excessive centrifugal force.
[0008] Preferably, the rotating disc is provided with placing groove at the position corresponding to culture dish, and the culture dish is arranged in the placing groove, the culture dish is arranged six, and is circumferentially distributed on the top of rotating disc, so as to limit the culture dish.
[0009] Preferably, the jacking mechanism comprises an observation bin, the observation bin is rotationally installed on the front of the cell culture box body, an electric push rod two is fixedly installed inside the observation bin, a jacking frame is fixedly installed at the output end of the electric push rod two, a connecting rod is rotationally installed on the top of the jacking frame, a jacking block is fixedly installed on the top of the jacking frame, and a sliding plate is rotationally installed at the end of the connecting rod away from the jacking frame, which avoids the pollution of cells in the culture dish by bacteria in the air, thereby improving the effect of cell culture.
[0010] Preferably, the connecting rod and the sliding plate are provided with two groups and are symmetrically distributed at the center line of the front of the observation bin, the culture dish is jacked up from the bottom of the placing groove by the jacking block, and the sliding plate is opened.
[0011] Preferably, a sliding groove is formed at the corresponding position of the observation bin and the sliding plate, and the sliding plate is slidingly installed in the sliding groove to limit the sliding plate.
[0012] Compared with the prior art, the utility model provides a large-scale cell culture device, which has the following beneficial effects:
[0013] 1. The large-scale cell culture device, by setting up the rotating mechanism, in the process of using, it is convenient to place the culture dish into the placing groove of the rotating disc, prevent the operator from affecting the line of sight after the operator's hand is put into the cell culture box body, and overturn other culture dishes, it is convenient for the operator to place the culture dish, the rotating disc is slowly rotated, and the centrifugal force of the cells in the culture dish is prevented from being too large to affect the cultivation effect.
[0014] 2. The large-scale cell culture device, by setting up the jacking mechanism, in the process of using, the culture dish is jacked up from the bottom of the placing groove by the jacking block, the sliding plate is opened, and then the nutrient solution is dropped into the culture dish, so that the culture dish does not need to be opened too large, thus avoiding the pollution of cells in the culture dish by bacteria in the air, thereby improving the effect of cell culture. BRIEF DESCRIPTION OF DRAWINGS
[0015] In order to more clearly illustrate the technical scheme in the embodiments of the utility model, the following will briefly introduce the drawings needed to be used in the embodiment description, and obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to these drawings without paying creative labor:
[0016] Figure 1 It is the appearance structure schematic diagram of the utility model;
[0017] Figure 2 It is the internal structure schematic diagram of the utility model;
[0018] Figure 3The rotating mechanism decomposition structure schematic view of the utility model;
[0019] Figure 4 The utility model provides a jacking mechanism appearance structure schematic view.
[0020] In the drawing: 1, cell culture box body; 2, rotating mechanism; 21, sliding frame; 22, electric push rod one; 23, motor; 24, gear; 25, rotating disc; 26, gear ring; 27, culture dish; 3, jacking mechanism; 31, observation bin; 32, electric push rod two; 33, jacking frame; 34, connecting rod; 35, sliding plate; 36, jacking block. DETAILED DESCRIPTION
[0021] The technical scheme in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the ordinary skilled in the art without creative work belong to the scope of the utility model protection.
[0022] In the utility model, unless another explicit provision and limitation, the terms "installation", "connection", "connection", "fixing" and other terms should be broad understanding, for example, can be fixed connection, can be detachable connection, or integral; can be mechanical connection, can be electrical connection; can be directly connected, can be indirectly connected through intermediate medium, can be the communication of two elements or the interaction of two elements. For the ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.
[0023] Embodiment one:
[0024] Please refer to Figures 1-3 The utility model provides a technical scheme: a large-scale cell culture device, including cell culture box body 1, cell culture box body 1 inside is provided with rotating mechanism 2, cell culture box body 1 inside is provided with jacking mechanism 3.
[0025] Further, rotating mechanism 2 includes sliding frame 21, sliding frame 21 is slidably installed in the inside of cell culture box body 1, cell culture box body 1 inside is fixedly installed with electric push rod one 22, the bottom of sliding frame 21 is fixedly installed with motor 23, the output end of motor 23 is fixedly installed with gear 24, the top of sliding frame 21 is rotatably installed with rotating disc 25, the outside of rotating disc 25 is fixedly installed with gear ring 26, and the top of rotating disc 25 is provided with culture dish 27, which is convenient to place culture dish 27 into the placing groove of rotating disc 25, and prevents the operator from affecting the line of sight after the hand is inserted into the inside of cell culture box body 1.
[0026] Further, the output end of the electric push rod 22 is fixedly installed with the sliding frame 21, the gear 24 is engaged with the gear ring 26, the rotating disc 25 is slowly rotated, and the cells in the culture dish 27 are prevented from being affected by the centrifugal force to affect the cultivation effect.
[0027] Further, the rotating disc 25 is provided with a placing groove at the corresponding position of the culture dish 27, the culture dish 27 is arranged in the placing groove, the culture dish 27 is arranged in six, and the culture dish 27 is circumferentially arranged on the top of the rotating disc 25, so that the culture dish 27 is limited.
[0028] Example two:
[0029] Please refer to Figure 4 , and further, the jacking mechanism 3 comprises an observation bin 31, the observation bin 31 is rotatably installed on the front surface of the cell culture box body 1, the electric push rod 32 is fixedly installed in the observation bin 31, the output end of the electric push rod 32 is fixedly installed with the jacking frame 33, the jacking frame 33 is rotatably installed with the connecting rod 34 on the top, the jacking frame 33 is fixedly installed with the jacking block 36 on the top, the connecting rod 34 is rotatably installed with the sliding plate 35 at the end away from the jacking frame 33, so as to avoid the bacteria in the air from falling into the culture dish 27 to pollute the cell tissue, thereby improving the effect of cell culture.
[0030] Further, the connecting rod 34 and the sliding plate 35 are provided with two groups, and are symmetrically distributed with the center line of the front surface of the observation bin 31, the culture dish 27 is jacked up from the bottom of the placing groove by the jacking block 36, and the sliding plate 35 is opened.
[0031] Further, the observation bin 31 is provided with a sliding groove at the corresponding position of the sliding plate 35, and the sliding plate 35 is slidably installed in the sliding groove, so as to limit the sliding plate 35.
[0032] In actual operation, when the device is used, the observation bin 31 is opened, the electric push rod 22 is started, the electric push rod 22 pushes the sliding frame 21 to slide in the cell culture box body 1, and then the sliding frame 21 is pushed out of the cell culture box body 1, so that the culture dish 27 is placed in the placing groove of the rotating disc 25, the operator's line of sight is not affected after the operator's hand is inserted into the cell culture box body 1, other culture dishes 27 are not overturned, the operator can place the culture dish 27 conveniently, after the placement is completed, the observation bin 31 is closed, when observation is needed, the electric push rod 22 is started, the electric push rod 22 pushes the sliding frame 21, the rotating disc 25 is moved into the observation bin 31, the motor 23 is started, the motor 23 drives the gear 24 to rotate, the gear 24 drives the gear ring 26 meshed with the gear 24 to rotate, and then the rotating disc 25 is slowly rotated, the cells in the culture dish 27 are prevented from being affected by excessive centrifugal force, the culture effect is prevented from being affected, the culture dish 27 can be observed and recorded from the top of the observation bin 31, when nutrient solution needs to be placed, the electric push rod 32 is started, the electric push rod 32 pushes the jacking frame 33 to move, and then the jacking block 36 is used to jack the culture dish 27 from the bottom of the placing groove, the jacking frame 33 is moved to move the two connecting rods 34 outward, the sliding plate 35 is opened, and then the nutrient solution is dropped into the culture dish 27, a large opening is not needed, and the sliding plate 35 is moved when the culture dish 27 is jacked, so that bacteria in the air is prevented from falling into the culture dish 27 and polluting the cell tissue, and the cell culture effect is improved.
[0033] It should be noted that the relational terms herein such as first and second and the like are used solely to distinguish one entity or action from another, without necessarily requiring or implying any such actual relationship or order between such entities or actions. Moreover, the terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element preceded by "comprises... " does not, without more constraints, preclude the existence of additional identical elements in the process, method, article, or apparatus that comprises the element.
Claims
1. A large-scale cell culture apparatus comprising a cell culture cabinet body (1), characterized in that: The cell culture box body (1) is internally provided with a rotating mechanism (2), and the cell culture box body (1) is internally provided with a jacking mechanism (3).
2. The large-scale cell culture device of claim 1, wherein: The rotating mechanism (2) comprises a sliding frame (21) which is slidingly installed in the cell culture box body (1), and the cell culture box body (1) is fixedly installed with an electric push rod (22) internally, the bottom of the sliding frame (21) is fixedly installed with a motor (23), the output end of the motor (23) is fixedly installed with a gear (24), the top of the sliding frame (21) is rotatably installed with a rotating disc (25), the outside of the rotating disc (25) is fixedly installed with a gear ring (26), and the top of the rotating disc (25) is provided with a culture dish (27).
3. The large-scale cell culture device of claim 2, wherein: The output end of the electric push rod (22) is fixedly installed with the sliding frame (21), and the gear (24) is engaged with the gear ring (26).
4. The large-scale cell culture device of claim 2, wherein: The rotating disc (25) is provided with a placing groove at the corresponding position of the culture dish (27), and the culture dish (27) is arranged in the placing groove, and the culture dish (27) is arranged in six, which are circumferentially distributed on the top of the rotating disc (25).
5. The large-scale cell culture device of claim 2, wherein: The jacking mechanism (3) comprises an observation bin (31) which is rotatably installed on the front face of the cell culture box body (1), and the observation bin (31) is fixedly installed with an electric push rod (32) internally, the output end of the electric push rod (32) is fixedly installed with a jacking frame (33), the top of the jacking frame (33) is rotatably installed with a connecting rod (34), the top of the jacking frame (33) is fixedly installed with a jacking block (36), and the end, away from the jacking frame (33), of the connecting rod (34) is rotatably installed with a sliding plate (35).
6. The large-scale cell culture device of claim 5, wherein: The connecting rod (34) and the sliding plate (35) are provided with two groups, and are symmetrically distributed at the center line of the front face of the observation bin (31).
7. The large-scale cell culture device of claim 5, wherein: The observation bin (31) is provided with a sliding groove at the corresponding position of the sliding plate (35), and the sliding plate (35) is slidingly installed in the sliding groove.