High performance liquid chromatograph for detecting concentration of vitamin C in human serum

Abstract

The utility model provides a high performance liquid chromatograph for detecting the concentration of vitamin C in human serum. Comprising a degasser, a detector arranged above the degasser, an automatic sample injector arranged above the detector, a solvent frame arranged above the automatic sample injector, a column oven arranged on the automatic sample injector, a side door hinged to the outer side of the degasser, a valve body fixedly installed on the inner side of the degasser and a valve rod installed in the valve body. And an exhaust driving mechanism mounted on the valve body is fixedly mounted on the inner side of the degasser. According to the high performance liquid chromatograph for detecting the human serum vitamin C concentration, a two-way lead screw is automatically matched with thread transmission to drive a first clamping plate and a second clamping plate to move reversely, and then a solvent bottle located on the inner side of an avoiding hole is clamped and fixed through an anti-skid pad; the vibration generated when the high performance liquid chromatograph is used cannot cause the shaking of the solvent bottle, so that the high performance liquid chromatograph can well limit the solvent bottle.

Description

TECHNICAL FIELD

[0001] The utility model relates to high performance liquid chromatograph technical field, specifically disclose a human serum vitamin C concentration detection uses high performance liquid chromatograph. BACKGROUND

[0002] Vitamin C (Vitamin C, Vc) is also called ascorbic acid, it is a water-soluble vitamin, Vc participates in a variety of reactions in the body, such as oxidation-reduction process, plays an important role in biological oxidation and reduction and cell respiration, lack of Vc in human body is easy to cause scurvy, simultaneously, because Vc is a water-soluble strong antioxidant and participates in the synthesis of collagen, it also has anticancer, prevents arteriosclerosis, treats anemia, antioxidant and improves human body immunity etc. Effect, can use high performance liquid chromatograph to detect the concentration of vitamin C in human serum.

[0003] The existing high performance liquid chromatograph in the process of using, solvent bottle is directly placed in the inside of solvent frame, because of the lack of the fixation of solvent bottle, the vibration generated when using high performance liquid chromatograph can cause the solvent bottle to shake, it can not be well limited to the solvent bottle, and before using high performance liquid chromatograph each time, the exhaust valve on the degassing machine is manually opened to exhaust, and after exhausting, the exhaust valve also needs to be manually closed, it is more troublesome to use. UTILITY MODEL CONTENT

[0004] In view of the above defects or deficiencies in the prior art, the present application aims to provide a human serum vitamin C concentration detection uses high performance liquid chromatograph, including degassing machine, the detector is arranged above the degassing machine, the automatic sampler is arranged above the detector, the solvent frame is arranged above the automatic sampler, the column oven is arranged on the automatic sampler, the side door is hinged on the outside of the degassing machine, and the valve body is fixedly installed on the inside of the degassing machine, and the valve rod is installed in the valve body, the inside of the degassing machine is fixedly installed with the exhaust driving mechanism installed on the valve body, the top of the automatic sampler is fixedly installed with the U-shaped plate that is equidistant and symmetrically distributed, and the top of the automatic sampler is fixedly installed with the mounting plate located outside the U-shaped plate, the inside of the mounting plate is sleeved with the clamping mechanism located inside the U-shaped plate, the top of the U-shaped plate is fixedly installed with the protective plate located above the mounting plate, the inside of the protective plate is clamped with the position-avoiding hole that is equidistant and symmetrically distributed, and the outside of the side door is fixedly installed with the control switch.

[0005] Preferably, the exhaust driving mechanism comprises a motor fixedly installed inside the degassing machine and electrically connected with the control switch, and a first fixed plate, a second fixed plate and a third fixed plate fixedly installed outside the valve body, a worm rotatably connected between the first fixed plate and the second fixed plate, a worm wheel rotatably connected inside the third fixed plate and sleeved outside the valve rod and in mesh transmission with the worm, and the worm extending out of the top end of the first fixed plate and fixedly sleeved with the output shaft of the motor.

[0006] Preferably, the inside of the worm wheel is clamped with a first guide groove matched with the valve rod.

[0007] Preferably, the clamping mechanism comprises a first fixed rod and a second fixed rod movably sleeved inside the mounting plate, and a bidirectional screw rod rotatably connected inside the middle part of the mounting plate, one end of the first fixed rod and the second fixed rod extending out of the mounting plate is movably sleeved with a first clamping plate clamped inside the U-shaped plate, and the other end of the first fixed rod and the second fixed rod extending out of the first clamping plate is fixedly sleeved with a second clamping plate clamped inside the U-shaped plate, the outside of the first clamping plate is provided with a U-shaped bracket threadedly sleeved with one end of the bidirectional screw rod, the inside of the first clamping plate and the second clamping plate is provided with an anti-skid pad, the other end of the first fixed rod and the second fixed rod extending out of the mounting plate is fixedly sleeved with a moving plate threadedly sleeved with the other end of the bidirectional screw rod, and one end of the bidirectional screw rod extending out of the moving plate is fixedly sleeved with a handle located below the protective plate.

[0008] Preferably, the inside of the U-shaped plate is provided with a second guide groove matched with the first clamping plate and the second clamping plate. Advantages

[0009] 1. The human serum vitamin C concentration detection high-performance liquid chromatograph, through the automatic bidirectional screw rod, the first clamping plate and the second clamping plate are reversely moved under the cooperation of the threaded transmission, and then the solvent bottle located inside the avoiding hole is clamped and fixed through the anti-skid pad, so that the vibration generated by the high-performance liquid chromatograph during use does not cause the solvent bottle to shake, thereby ensuring that the high-performance liquid chromatograph can well limit the solvent bottle.

[0010] 2. The human serum vitamin C concentration detection high-performance liquid chromatograph, through the control switch, the operator can directly control the motor to drive outside the high-performance liquid chromatograph while rotating the valve rod through the cooperation of the worm and the worm wheel, and then automatically opening or closing the exhaust valve inside the valve body, which is convenient to use, and the mesh transmission between the worm and the worm wheel can be self-locked, thereby ensuring that the exhaust valve inside the valve body will not be opened or closed automatically. BRIEF DESCRIPTION OF DRAWINGS

[0011] Other features, objects, and advantages of the application will become more apparent from the following detailed description of non-limiting embodiments thereof, when read in connection with the following drawings:

[0012] Figure 1 It is a schematic view of the structure of the utility model;

[0013] Figure 2 It is a schematic view of the structure of the utility model;

[0014] Figure 3 It is a schematic view of the solvent rack of the utility model;

[0015] Figure 4 It is Figure 2 It is an enlarged schematic view of the structure at A in the middle;

[0016] Figure 5 It is a schematic view of the worm gear of the utility model;

[0017] Figure 6 It is a schematic view of the clamping mechanism of the utility model.

[0018] In the figure: 1, degassing machine; 2, detector; 3, automatic sampler; 4, solvent rack; 5, column temperature box; 6, side door; 7, valve body; 8, valve stem; 9, exhaust driving mechanism; 91, motor; 92, first fixed plate; 93, second fixed plate; 94, third fixed plate; 95, worm; 96, worm gear; 97, shaft sleeve; 10, U-shaped plate; 11, mounting plate; 12, clamping mechanism; 121, first fixed rod; 122, second fixed rod; 123, bidirectional screw; 124, first clamping plate; 125, second clamping plate; 126, U-shaped frame; 127, non-slip pad; 128, moving plate; 129, handle; 13, protective plate; 14, avoidance hole; 15, control switch; 16, first guide groove; 17, second guide groove. DETAILED DESCRIPTION

[0019] The application will be further described below in connection with the drawings and embodiments. It can be understood that the specific embodiments described herein are only used to explain the related utility model, and not to limit the utility model. In addition, it should be noted that, in order to facilitate the description, only the parts related to the utility model are shown in the drawings.

[0020] The drawings in the embodiments of the utility model: different kinds of section lines in the figure are not marked according to the national standard, and the material of the element is not required, which is to distinguish the sectional view of the element in the figure.

[0021] Please refer to Figures 1-6The utility model provides a kind of human serum Vitamin C concentration detection with high performance liquid chromatograph, including degassing machine 1, the detector 2 being arranged above degassing machine 1, the autosampler 3 being arranged above detector 2, the solvent rack 4 being arranged above autosampler 3, the column oven 5 being arranged on autosampler 3, the side door 6 being hinged on the outside of degassing machine 1 and the valve body 7 being fixedly installed in the inside of degassing machine 1 and the valve stem 8 being installed in the inside of valve body 7, the inside of degassing machine 1 is fixedly installed with exhaust driving mechanism 9 installed on valve body 7, the top of autosampler 3 is fixedly installed with U-shaped plate 10 that is equidistant and symmetrically distributed, and the top of autosampler 3 is fixedly installed with mounting plate 11 located outside U-shaped plate 10, the inside of mounting plate 11 is equipped with clamping mechanism 12 located inside U-shaped plate 10, the top of U-shaped plate 10 is fixedly installed with guard plate 13 located above mounting plate 11, clamping mechanism 12 can be shielded by guard plate 13, the inside of guard plate 13 is equipped with equal interval and symmetrically distributed avoidance hole 14, solvent bottle can be placed inside the top of solvent rack 4 by avoiding hole 14, the outside of side door 6 is fixedly installed with control switch 15, so that operating personnel can control the drive of motor 91 outside high performance liquid chromatograph by control switch 15.

[0022] Wherein, exhaust driving mechanism 9 includes motor 91 fixedly installed in the inside of degassing machine 1 and electrically connected between control switch 15, and first fixed plate 92, second fixed plate 93 and third fixed plate 94 fixedly installed on the outside of valve body 7, first fixed plate 92 and second fixed plate 93 are rotatably connected with worm 95, third fixed plate 94 is rotatably connected with worm gear 96 sleeved on the outside of valve stem 8 and engaged with worm 95, the top outside of first fixed plate 92 extending out is fixedly sleeved between the output shaft of motor 91 through shaft sleeve 97, the rotation of worm 95 can be driven by motor 91, and worm gear 96 is rotated around its own axis, and the exhaust valve in the inside of valve body 7 can be opened or closed by valve stem 8.

[0023] Wherein, the inside of worm gear 96 is equipped with first guide groove 16 matched with valve stem 8, the axial movement of valve stem 8 is not interfered with by first guide groove 16, so that valve stem 8 is synchronously rotated with worm gear 96.

[0024] The clamping mechanism 12 comprises a first fixed rod 121 and a second fixed rod 122 movably sleeved inside the mounting plate 11 and a bidirectional screw rod 123 rotatably connected inside the middle part of the mounting plate 11, the first fixed rod 121 and the second fixed rod 122 movably sleeved outside one end of the mounting plate 11 are movably sleeved with a first clamping plate 124 clamped inside the U-shaped plate 10, and the first fixed rod 121 and the second fixed rod 122 movably sleeved outside one end of the first clamping plate 124 are movably sleeved with a second clamping plate 125 clamped inside the U-shaped plate 10, the outside of the first clamping plate 124 is provided with a U-shaped frame 126 sleeved outside one end of the bidirectional screw rod 123, the inside of the first clamping plate 124 and the second clamping plate 125 are provided with anti-skid pads 127, the friction between the first clamping plate 124, the second clamping plate 125 and the solvent bottle can be increased through the anti-skid pads 127, so that the solvent bottle is not easy to separate from the inside of the first clamping plate 124 and the second clamping plate 125 after being clamped and fixed, the other end of the first fixed rod 121 and the second fixed rod 122 extending outside the mounting plate 11 is movably sleeved with a moving plate 128 sleeved outside the other end of the bidirectional screw rod 123, the U-shaped frame 126 and the moving plate 128 can be moved in opposite directions through the rotation of the bidirectional screw rod 123, and the second clamping plate 125 and the first clamping plate 124 can be moved in opposite directions synchronously through the first fixed rod 121 and the second fixed rod 122, one end of the bidirectional screw rod 123 extending outside the moving plate 128 is movably sleeved with a handle 129 below the protective plate 13, and the handle 129 is convenient for rotating the bidirectional screw rod 123.

[0025] The inside of the U-shaped plate 10 is provided with a second guide groove 17 matched with the first clamping plate 124 and the second clamping plate 125, the first clamping plate 124 and the second clamping plate 125 can be limited and guided through the second guide groove 17, further avoiding the solvent bottle clamped between them from shaking, and the first clamping plate 124 and the second clamping plate 125 can stably move inside the U-shaped plate 10 under the rotation of the bidirectional screw rod 123.

[0026] When the solvent bottle is clamped, the solvent bottle is placed on the solvent rack 4 through the avoiding hole 14 one by one, the handle 129 is rotated to drive the U-shaped frame 126 and the moving plate 128 to move reversely, the U-shaped frame 126 drives the first clamping plate 124 to move along the inner side of the second guide groove 17 synchronously, the moving plate 128 drives the second clamping plate 125 to move along the inner side of the second guide groove 17 synchronously through the first fixed rod 121 and the second fixed rod 122, the first clamping plate 124 and the second clamping plate 125 move to clamp and fix the solvent bottle through the non-slip pad 127, and the clamping of the solvent bottle is completed, and the contents not described in detail in the description belong to the prior art known to the person skilled in the art.

[0027] It should be noted that the embodiments and the features in the embodiments in the present application can be combined with each other without conflict. The present application will be described in detail below with reference to the drawings and in combination with the embodiments.

[0028] The above description is only the preferred embodiments of the present application and the explanation of the applied technical principles. It should be understood by those skilled in the art that the utility model range involved in the present application is not limited to the technical solutions formed by the specific combination of the above technical features, and should also cover other technical solutions formed by the combination of the above technical features or their equivalent features without departing from the utility model concept. For example, the technical solutions formed by the mutual replacement of the above features and the technical features disclosed in the present application (but not limited to) having similar functions.

Claims

1. A high-performance liquid chromatograph for detecting vitamin C concentration in human serum, comprising a degasser (1), a detector (2) disposed above the degasser (1), an autosampler (3) disposed above the detector (2), a solvent rack (4) disposed above the autosampler (3), a column oven (5) disposed on the autosampler (3), a side door (6) hinged to the outside of the degasser (1), a valve body (7) fixedly installed inside the degasser (1), and a valve stem (8) installed inside the valve body (7), characterized in that: The inside of the degassing machine (1) is fixedly installed with an exhaust driving mechanism (9) installed on the valve body (7), the top of the automatic sampler (3) is fixedly installed with U-shaped plates (10) that are equidistant and symmetrically distributed, and the top of the automatic sampler (3) is fixedly installed with a mounting plate (11) located outside the U-shaped plates (10), the inside of the mounting plate (11) is sleeved with a clamping mechanism (12) located inside the U-shaped plates (10), the top of the U-shaped plates (10) is fixedly installed with a protective plate (13) located above the mounting plate (11), the inside of the protective plate (13) is clamped with position-avoiding holes (14) that are equidistant and symmetrically distributed, and the outside of the side door (6) is fixedly installed with a control switch (15).

2. The high performance liquid chromatograph for detecting the concentration of vitamin C in human serum according to claim 1, characterized in that: The exhaust driving mechanism (9) comprises a motor (91) fixedly installed on the inside of the degassing machine (1) and electrically connected with the control switch (15), and a first fixed plate (92), a second fixed plate (93) and a third fixed plate (94) fixedly installed on the outside of the valve body (7), a worm (95) rotatably connected between the first fixed plate (92) and the second fixed plate (93), a worm wheel (96) rotatably connected inside the third fixed plate (94) and sleeved on the outside of the valve rod (8) and in meshing transmission with the worm (95), and the top end outside the first fixed plate (92) extends outside and is fixedly sleeved between the output shaft of the motor (91) through a shaft sleeve (97).

3. The high performance liquid chromatograph for detecting the concentration of vitamin C in human serum according to claim 2, characterized in that: The inside of the worm wheel (96) is clamped with a first guide groove (16) matched with the valve rod (8).

4. The high performance liquid chromatograph for detecting the concentration of vitamin C in human serum according to claim 1, characterized in that: The clamping mechanism (12) comprises a first fixed rod (121) and a second fixed rod (122) movably sleeved inside the mounting plate (11), and a bidirectional screw rod (123) rotatably connected inside the middle part of the mounting plate (11), one end of the first fixed rod (121) and the second fixed rod (122) extending outside the mounting plate (11) is movably sleeved with a first clamping plate (124) clamped inside the U-shaped plate (10), and the other end of the first fixed rod (121) and the second fixed rod (122) extending outside the first clamping plate (124) is fixedly sleeved with a second clamping plate (125) clamped inside the U-shaped plate (10), the outside of the first clamping plate (124) is provided with a U-shaped frame (126) threadedly sleeved on one end of the bidirectional screw rod (123), the inside of the first clamping plate (124) and the second clamping plate (125) is provided with an antiskid pad (127), the other end of the first fixed rod (121) and the second fixed rod (122) extending outside the mounting plate (11) is fixedly sleeved with a moving plate (128) threadedly sleeved on the other end of the bidirectional screw rod (123), and one end of the bidirectional screw rod (123) extending outside the moving plate (128) is fixedly sleeved with a handle (129) located below the protective plate (13).

5. The high performance liquid chromatograph for detecting the concentration of vitamin C in human serum according to claim 4, wherein: The inside of the U-shaped plate (10) is provided with a second guide groove (17) matched with the first clamping plate (124) and the second clamping plate (125).

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