A device for detecting visceral pain behavior in mice

Abstract

This invention belongs to the field of detection devices, specifically a detection device for visceral pain behavior in mice. It includes a housing with a pressure detector mounted on its inner wall; a three-way valve at the top of the housing; the three-way valve and the pressure detector connected via a pipe; a connecting component on the surface of the three-way valve; a syringe at the top of the connecting component; a balloon on one side of the three-way valve; the balloon and the three-way valve connected via a pipe; an elastic frame at the top of the syringe; the elastic frame has a C-shaped structure; and a first clamp is fixed to the end of the elastic frame. By observing the pressure detector, the internal pressure of the balloon can be accurately assessed, enabling the device to quantify the degree of pain in mice, better understand the relationship between input gas pressure and the degree of visceral pain in mice, and facilitate subsequent research.

Description

Technical Field

[0001] This invention relates to the field of detection devices, specifically a detection device for visceral pain behavior in mice. Background Technology

[0002] Visceral pain is characterized by inaccurate localization, long duration, dulled pain sensation, and is often accompanied by other symptoms. It may cause emotional reactions, manifesting as irritability, restlessness, tension, etc. Excitation of the sympathetic and parasympathetic nervous systems may also cause symptoms such as nausea, vomiting, dizziness, and sweating.

[0003] Mice are commonly used as model organisms in the study of visceral pain. The outstanding value of mice as model organisms lies mainly in their high homology with humans in their genome, strong reproductive capacity, similar physiological and biochemical indicators to humans, mature gene modification technology, and the various inbred strains developed for experiments. Therefore, mice are widely used in the study of visceral pain.

[0004] Existing methods for studying visceral pain are limited. For example, stomach pain behavior is usually detected by gastric distension test, which requires implanting a gastric balloon in the stomach of mice. However, there is a lack of precise indicators to measure the degree of visceral pain induced by balloon distension in mice.

[0005] Therefore, a device for detecting visceral pain behavior in mice is proposed to address the above-mentioned problems. Utility Model Content

[0006] In order to overcome the shortcomings of the prior art, at least one technical problem raised in the background art is solved.

[0007] The technical solution adopted by this utility model to solve its technical problem is as follows: A detection device for visceral pain behavior in mice, comprising a box body, with a pressure detector installed on the inner wall of the box body; a three-way valve is provided on the top of the box body; the three-way valve and the pressure detector are connected by a pipe; a connecting component is provided on the surface of the three-way valve; a syringe is provided on the top of the connecting component; a balloon is provided on one side of the three-way valve; the balloon and the three-way valve are connected by a pipe; by observing the pressure detector, the internal pressure of the balloon can be accurately assessed, realizing the quantification of the degree of pain in mice, better understanding the relationship between input gas pressure and the degree of visceral pain in mice, facilitating subsequent research.

[0008] Preferably, the syringe is provided with an elastic frame at the top; the elastic frame has a C-shaped structure; a first clamp is fixedly connected to the end of the elastic frame; the syringe is located between a pair of first clamps; a first screw is threadedly connected to the middle of the elastic frame; by rotating the first screw, the first screw can slowly squeeze the syringe, thereby realizing precise control of the air pressure inside the balloon by the device, and improving the accuracy and reliability of the device's detection results.

[0009] Preferably, a plurality of elastic wires are fixedly connected between a pair of first clamps; the elastic wires are equidistantly arranged; when the worker installs the elastic frame, it is necessary to pull the two sides of the elastic frame to make the elastic frame in an expanded state. When the elastic frame expands, the length of the elastic wires will also be stretched. The elastic frame will be subjected to the elastic force of the elastic wires, so that the elastic frame will be subject to more resistance when it expands, thereby reducing the structural damage caused by excessive expansion of the elastic frame.

[0010] Preferably, the connecting assembly includes a fixing plate; the fixing plate and the three-way valve are fixedly connected; a plurality of second screws are threadedly connected to the middle of the fixing plate; a second clamping plate is rotatably connected to the end of the second screw; the second clamping plate and the fixing plate are slidably connected; a positioning hole is provided on the inner sidewall of the fixing plate; the positioning hole and the three-way valve are connected; through the cooperation of the second screws and the second clamping plate, the device can fix syringes of different sizes, improving the flexibility and stability of the device in fixing syringes.

[0011] Preferably, a plurality of rubber pads are fixedly connected to the middle of the second clamping plate; the surface of the rubber pads has an arc-shaped structure; when the second clamping plate contacts the outer wall of the syringe, the rubber pads will also contact the syringe. Because the surface of the rubber pads has a flexible structure, the rubber pads will reduce the direct contact between the syringe and the second clamping plate, reduce the wear on the surface of the syringe caused by compression, and at the same time, the rubber pads will fill the gap between the second clamping plate and the syringe, and enhance the squeezing effect of the second clamping plate on the syringe.

[0012] Preferably, multiple sealing gaskets are fixed to the inner wall of the positioning hole; the sealing gaskets are flexible structures; when the syringe passes through the positioning hole, the syringe will squeeze the multiple sealing gaskets to deform them; when the device stops working, the sealing gaskets will recover due to their own memory and seal the positioning hole, reducing the possibility of external impurities entering the three-way valve.

[0013] The advantages of this utility model are:

[0014] 1. The present invention provides a detection device for visceral pain behavior in mice. By observing the air pressure detector, the internal air pressure of the balloon can be accurately assessed, thereby quantifying the degree of pain in mice and better understanding the relationship between input gas pressure and the degree of visceral pain in mice, which is convenient for subsequent research.

[0015] 2. The detection device for visceral pain behavior in mice described in this utility model can achieve precise control of the air pressure inside the balloon by rotating the first screw, thereby improving the accuracy and reliability of the detection results. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the main body of this utility model;

[0018] Figure 2 This is a schematic diagram of the three-way valve in this utility model;

[0019] Figure 3 This is a schematic diagram of the elastic frame in this utility model;

[0020] Figure 4 This is a schematic diagram of the elastic wire in this utility model;

[0021] Figure 5 This is a schematic diagram of the structure of the second clamping plate in this utility model.

[0022] In the diagram: 1. Box body; 12. Air pressure detector; 13. Three-way valve; 14. Balloon; 15. Connecting assembly; 16. Syringe; 2. Elastic frame; 22. First clamping plate; 23. First screw; 3. Elastic wire; 4. Fixing plate; 42. Second screw; 43. Second clamping plate; 44. Positioning hole; 5. Rubber pad; 6. Sealing gasket. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present utility model.

[0024] Specific implementation examples are given below.

[0025] Please see Figures 1 to 5As shown in the embodiment of this utility model, a detection device for visceral pain behavior in mice includes a housing 1, with a barometer 12 installed on the inner wall of the housing 1; a three-way valve 13 is provided on the top of the housing 1; the three-way valve 13 and the barometer 12 are connected by a pipe; a connecting component 15 is provided on the surface of the three-way valve 13; a syringe 16 is provided on the top of the connecting component 15; a balloon 14 is provided on one side of the three-way valve 13; the balloon 14 and the three-way valve 13 are connected by a pipe; during operation, the balloon 14 can be implanted into the mouse whose visceral pain behavior needs to be detected, specifically in the visceral organ to be detected, such as the stomach or colon and rectum. Then, the experimenter can connect the syringe 16 and the three-way valve 13 through the connecting component 15, and then press... The syringe 16 allows gas inside to enter the balloon 14 through the three-way valve 13. The gas causes the balloon 14 to expand, inducing a pain response in the mouse's internal organs. Since the balloon 14 and the pressure detector 12 are connected through the three-way valve 13, and the pressure detector 12 can be a mercury gauge, the operator can determine the specific gas pressure inside the balloon 14 by observing the height of the mercury column on the surface of the pressure detector 12. After controlling the gas pressure inside the balloon 14 to the required value, the experimenter can observe the mouse's behavior to determine the mouse's visceral pain behavior. By observing the pressure detector 12, the gas pressure inside the balloon 14 can be accurately assessed, enabling the device to quantify the degree of pain in the mouse, better understand the relationship between the input gas pressure and the degree of visceral pain in the mouse, and facilitate subsequent research.

[0026] Please see Figure 3 and Figure 4 As shown, the syringe 16 has an elastic frame 2 at its top; the elastic frame 2 has a C-shaped structure; a first clamping plate 22 is fixedly connected to the end of the elastic frame 2; the syringe 16 is located between a pair of first clamping plates 22; a first screw 23 is threadedly connected to the middle of the elastic frame 2; after aligning the first clamping plate 22 and the syringe 16, the operator can put the elastic frame 2 on the surface of the syringe 16. The first clamping plate 22 will clamp the surface of the syringe 16 under the elastic force of the elastic frame 2. When the operator adjusts the air pressure inside the balloon 14, he can first press the syringe 16 normally to deliver air into the balloon 14. When the air pressure inside the balloon 14... When the target value is approaching, the experimenter can rotate the first screw 23 to slide it along the elastic frame 2. The first screw 23 will slowly compress the syringe 16, causing the air pressure inside the balloon 14 to slowly increase. The air pressure inside the balloon 14 can be judged by the mercury column height on the surface of the barometer 12. After the experiment, the staff can pull the elastic frame 2 apart from both sides to separate the elastic frame 2 from the syringe 16. By rotating the first screw 23, the first screw 23 can slowly compress the syringe 16, realizing precise control of the air pressure inside the balloon 14 by the device, improving the accuracy and reliability of the device's detection results.

[0027] Please see Figure 4 As shown, a plurality of elastic wires 3 are fixed between a pair of first clamping plates 22; the elastic wires 3 are arranged at equal intervals; when the worker installs the elastic frame 2, it is necessary to pull the two sides of the elastic frame 2 to make the elastic frame 2 in an expanded state. When the elastic frame 2 expands, the length of the elastic wires 3 will also be stretched. The elastic frame 2 will be subjected to the elastic force of the elastic wires 3, so that the elastic frame 2 will be subject to more resistance when it expands, thereby reducing the structural damage caused by excessive expansion of the elastic frame 2.

[0028] Please see Figure 5 As shown, the connecting assembly 15 includes a fixing plate 4; the fixing plate 4 and the three-way valve 13 are fixedly connected; a plurality of second screws 42 are threadedly connected to the middle of the fixing plate 4; a second clamping plate 43 is rotatably connected to the end of the second screw 42; the second clamping plate 43 and the fixing plate 4 are slidably connected; a positioning hole 44 is provided on the inner sidewall of the fixing plate 4; the positioning hole 44 and the three-way valve 13 are in communication; when the operator needs to connect the syringe 16 and the three-way valve 13, the syringe 16 can be aligned with the positioning hole 44 and then... The syringe 16 is inserted into the fixed plate 4, and then multiple second screws 42 can be rotated to make the second screws 42 slide along the fixed plate 4 with the second clamping plate 43 until the second clamping plate 43 comes into contact with the syringe 16. The syringe 16 will be fixed under the squeezing action of the multiple second clamping plates 43, and then the balloon 14 can be inflated through the syringe 16. Through the cooperation of the second screws 42 and the second clamping plate 43, the device can fix syringes 16 of different sizes, improving the flexibility and stability of the device in fixing syringes 16.

[0029] Please see Figure 5 As shown, a plurality of rubber pads 5 are fixedly connected to the middle of the second clamping plate 43; the surface of the rubber pad 5 is an arc-shaped structure; when the second clamping plate 43 contacts the outer wall of the syringe 16, the rubber pad 5 will also contact the syringe 16. Because the surface of the rubber pad 5 is a flexible structure, the rubber pad 5 will reduce the direct contact between the syringe 16 and the second clamping plate 43, reduce the wear on the surface of the syringe 16 caused by compression, and at the same time, the rubber pad 5 will fill the gap between the second clamping plate 43 and the syringe 16, and enhance the squeezing effect of the second clamping plate 43 on the syringe 16.

[0030] Please see Figure 5 As shown, multiple sealing gaskets 6 are fixed to the inner wall of the positioning hole 44; the sealing gaskets 6 are flexible structures; when the syringe 16 passes through the positioning hole 44, the syringe 16 will squeeze the multiple sealing gaskets 6 to deform them. When the device stops working, the sealing gaskets 6 will recover due to their own memory and seal the positioning hole 44, reducing the possibility of external impurities entering the three-way valve 13.

[0031] Please see Figure 5 As shown, the inner wall of the fixing plate 4 is inclined. Because the inner wall of the fixing plate 4 is inclined, the syringe 16 will slide along the inner wall of the fixing plate 4 when it enters the fixing plate 4 until the axis of the syringe 16 and the fixing plate 4 are collinear, which improves the accuracy of the position when the syringe 16 and the three-way valve 13 are docked.

[0032] Working principle: The balloon 14 is implanted into the mouse whose visceral pain behavior needs to be detected, specifically in the visceral organ to be tested, such as the stomach or colon / rectum. Then, the experimenter connects the syringe 16 and the three-way valve 13 via the connecting component 15. Pressing the syringe 16 allows gas inside the syringe to enter the balloon 14 through the three-way valve 13. The gas causes the balloon 14 to expand, inducing a pain response in the mouse's viscera. Because the balloon 14 and the pressure detector 12 are connected via the three-way valve 13 (the pressure detector 12 can be a mercury gauge), the experimenter can determine the specific gas pressure inside the balloon 14 by observing the height of the mercury column on the surface of the pressure detector 12, thus controlling the internal pressure of the balloon 14 at the required level. After the value is measured, the experimenter can observe the mouse's behavior to determine the mouse's visceral pain behavior. The operator can align the first clamp 22 and the syringe 16, and then place the elastic frame 2 on the surface of the syringe 16. The first clamp 22 will clamp the surface of the syringe 16 under the elastic force of the elastic frame 2. When adjusting the internal air pressure of the balloon 14, the operator can first press the syringe 16 normally to deliver air into the balloon 14. When the internal air pressure of the balloon 14 is close to the required value, the experimenter can rotate the first screw 23 so that the first screw 23 slides along the elastic frame 2. The first screw 23 will slowly compress the syringe 16, causing the internal air pressure of the balloon 14 to slowly increase. The internal air pressure of the balloon 14 can be determined by the mercury column height on the surface of the barometer 12. After the experiment, the staff can separate the elastic frame 2 from the syringe 16 by pulling it apart from both sides. When installing the elastic frame 2, the staff needs to pull both sides of the elastic frame 2 to make it expand. When the elastic frame 2 expands, the length of the elastic wire 3 will also be stretched. The elastic frame 2 will be subject to the elastic force of the elastic wire 3, resulting in more resistance when the elastic frame 2 expands. When the staff needs to connect the syringe 16 and the three-way valve 13, the staff can align the syringe 16 with the positioning hole 44 and insert it into the interior of the fixing plate 4. Then, the staff can rotate the second screws 42 to make the second screws 42 slide along the fixing plate 4 with the second clamping plate 43 until the second clamping plate 43 and the syringe 16 come into contact. The syringe 16 is fixed under the squeezing action of multiple second clamps 43, and then the balloon 14 can be inflated through the syringe 16. When the second clamps 43 and the outer wall of the syringe 16 come into contact, the rubber pad 5 also comes into contact with the syringe 16. Because the surface of the rubber pad 5 is a flexible structure, the rubber pad 5 reduces the direct contact between the syringe 16 and the second clamps 43, reduces the wear on the surface of the syringe 16 caused by squeezing, and at the same time, the rubber pad 5 also fills the gap between the second clamps 43 and the syringe 16. When the syringe 16 passes through the positioning hole 44, the syringe 16 squeezes multiple sealing pads 6 to deform them. When the device stops working, the sealing pads 6 will recover due to their own memory and seal the positioning hole 44.

[0033] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model.

Claims

1. A device for detecting visceral pain behavior in mice, comprising a housing (1), characterized in that: A pressure detector (12) is installed on the inner wall of the box (1); a three-way valve (13) is provided on the top of the box (1); the three-way valve (13) and the pressure detector (12) are connected by a pipe; a connecting component (15) is provided on the surface of the three-way valve (13); a syringe (16) is provided on the top of the connecting component (15); a balloon (14) is provided on one side of the three-way valve (13); the balloon (14) and the three-way valve (13) are connected by a pipe.

2. The detection device for visceral pain behavior in mice according to claim 1, characterized in that: The syringe (16) is provided with an elastic frame (2) at the top; the elastic frame (2) is a C-shaped structure; the end of the elastic frame (2) is fixedly connected to a first clamping plate (22); the syringe (16) is located between a pair of first clamping plates (22); the middle part of the elastic frame (2) is threadedly connected to a first screw (23).

3. The detection device for visceral pain behavior in mice according to claim 2, characterized in that: A plurality of elastic wires (3) are fixedly connected between a pair of first clamps (22); the elastic wires (3) are arranged at equal intervals.

4. The detection device for visceral pain behavior in mice according to claim 3, characterized in that: The connecting assembly (15) includes a fixing plate (4); the fixing plate (4) and the three-way valve (13) are fixedly connected; a plurality of second screws (42) are threadedly connected to the middle part of the fixing plate (4); a second clamping plate (43) is rotatably connected to the end of the second screw (42); the second clamping plate (43) and the fixing plate (4) are slidably connected; a positioning hole (44) is provided on the inner side wall of the fixing plate (4); the positioning hole (44) and the three-way valve (13) are connected.

5. The detection device for visceral pain behavior in mice according to claim 4, characterized in that: The second clamping plate (43) has multiple rubber pads (5) fixed in the middle; the surface of the rubber pads (5) is an arc-shaped structure.

6. The detection device for visceral pain behavior in mice according to claim 5, characterized in that: Multiple sealing gaskets (6) are fixed to the inner wall of the positioning hole (44); the sealing gaskets (6) are flexible structures.

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