A rapid nucleic acid detector calibration device

Abstract

This invention provides a rapid nucleic acid detection instrument calibration device, including a temperature probe. Within the temperature probe, corresponding to the nucleic acid detection instrument, are arranged from top to bottom a nucleic acid lysis zone, a nucleic acid washing zone, and a nucleic acid amplification detection zone. At the center of each of the three zones, a first temperature sensor for detecting the temperature of the nucleic acid lysis zone, a second temperature sensor for detecting the temperature of the nucleic acid washing zone, and a third temperature sensor for detecting the temperature of the nucleic acid amplification detection zone are respectively located. This invention uses three different temperature sensors within the same temperature probe, corresponding to different detection zones of the instrument, to effectively detect temperature field changes in different areas.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid testing instrument calibration technology, specifically to a rapid nucleic acid testing instrument calibration device. Background Technology

[0002] Rapid nucleic acid testing instruments involve nucleic acid extraction, which requires steps such as lysis and washing. Each step necessitates an independent reaction space, dividing the reagent tubes into multiple independent compartments, each requiring individual temperature control. Rapid nucleic acid testing instruments differ from PCR instruments. Traditional PCR instruments only perform gene amplification, requiring only one reaction space and specific reaction conditions. Furthermore, the temperature control of multiple reaction wells in a PCR instrument is controlled by a single temperature control plate, requiring only a few channels to be tested during the metrology process. In contrast, rapid nucleic acid testing instruments have independent temperature control modules for each channel, preventing interference. Therefore, calibration requires testing each channel to evaluate product performance, and commercially available nucleic acid testing instrument calibration equipment is not well-suited for this purpose.

[0003] In view of this, the inventors of this case conducted in-depth research on the above-mentioned problems, which led to the creation of this case. Summary of the Invention

[0004] The purpose of this invention is to address the above-mentioned shortcomings by providing a convenient and highly accurate rapid nucleic acid detection instrument calibration device.

[0005] The solution adopted by this utility model to solve the technical problem is: a rapid nucleic acid detection instrument calibration device, including a temperature probe. The temperature probe is provided with a nucleic acid lysis zone, a nucleic acid cleaning zone and a nucleic acid amplification detection zone arranged from top to bottom in relation to the nucleic acid detection instrument. At the center of the nucleic acid lysis zone, the nucleic acid cleaning zone and the nucleic acid amplification detection zone, a first temperature sensor for detecting the temperature of the nucleic acid lysis zone, a second temperature sensor for detecting the temperature of the nucleic acid cleaning zone and a third temperature sensor for detecting the temperature of the nucleic acid amplification detection zone are respectively provided.

[0006] Furthermore, the temperature probe includes a frustum-shaped tube with a diameter that gradually decreases from top to bottom, a cover that is detachably installed on the top of the frustum-shaped tube, and two insertion tubes extending downward from the bottom of the frustum-shaped tube. The nucleic acid lysis zone is located in the upper part of the frustum-shaped tube, the nucleic acid washing zone is located in the lower part of the frustum-shaped tube, and the nucleic acid amplification detection zone is located at the two insertion tubes.

[0007] The first temperature sensor is located at the center of the upper part of the frustum-shaped tube, the second temperature sensor is located at the center of the lower part of the frustum-shaped tube, and there are two third temperature sensors, which are respectively located on the upper side of the two insertion tubes. The cover body is provided with a circuit board connected to the first temperature sensor, the second temperature sensor and the third temperature sensor.

[0008] Furthermore, a first metal point is provided on the outer wall of the temperature probe corresponding to the nucleic acid lysis region, and the first metal point is connected to the first temperature sensor. A second metal point is provided on the outer wall of the temperature probe corresponding to the nucleic acid lysis region, and the second metal point is connected to the second temperature sensor. An outwardly extending metal probe is provided inside the cannula, and the metal probe is connected to the third temperature sensor.

[0009] Furthermore, both the first and second temperature sensors are provided with long metal wires that can be rolled up to ensure that the first and second temperature sensors are located at the center of the nucleic acid lysis region and the nucleic acid washing region, respectively.

[0010] Furthermore, the nucleic acid lysis zone, nucleic acid washing zone, and nucleic acid amplification and detection zone are each equipped with a temperature measuring medium, which is air, pure water, or gel.

[0011] Furthermore, the first temperature sensor, the second temperature sensor, and the third temperature sensor all employ platinum resistance sensors.

[0012] Furthermore, the temperature probe is provided in several parts, and the rapid nucleic acid detection instrument calibration device also includes a controller. The several temperature probes are all connected to the controller. The controller includes a housing, and the housing contains a control circuit board, a power module, a communication module and a wireless communication module. The power module, the communication module and the wireless communication module are all connected to the control circuit board. The housing is provided with several interfaces connected to the control circuit board for plugging in the temperature probes.

[0013] Compared with the prior art, this utility model has the following advantages: Within the same temperature probe, three different temperature sensors are set up corresponding to different detection zones of the detector to effectively detect temperature field changes in different areas. Furthermore, by setting long metal wires, the sensors can be adjusted to the center of the temperature measurement zone, thus effectively ensuring temperature measurement accuracy. Moreover, the selection of the sensors, from platinum resistance thermometers and thermocouples, chooses a platinum resistance thermometer with suitable temperature measurement accuracy and response rate, which can accurately and quickly respond to temperature changes in the rapid nucleic acid detector, thereby effectively ensuring calibration accuracy and guaranteeing the normal operation of the nucleic acid detector. Attached Figure Description

[0014] The present invention will be further described below with reference to the accompanying drawings and embodiments:

[0015] Figure 1 This is a schematic diagram of the temperature probe structure;

[0016] Figure 2 This is a cross-sectional view of the temperature probe.

[0017] Figure 3 This is a schematic diagram of the controller's structure.

[0018] In the figure: temperature probe 1; frustum-shaped tube 11; nucleic acid lysis zone 111; nucleic acid washing zone 112; nucleic acid amplification and detection zone 113; cover 12; insertion tube 13; first temperature sensor 2; second temperature sensor 3; third temperature sensor 4; first metal point 5; second metal point 6; metal probe 7; long metal wire 8. Detailed Implementation

[0019] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments:

[0020] Example: Figure 1-3 As shown, this embodiment provides a rapid nucleic acid detection instrument calibration device, including a temperature probe 1. The temperature probe 1 has a nucleic acid lysis zone 111, a nucleic acid washing zone 112, and a nucleic acid amplification detection zone 113 arranged sequentially from top to bottom corresponding to the nucleic acid detection instrument. At the center of the nucleic acid lysis zone 111, the nucleic acid washing zone 112, and the nucleic acid amplification detection zone 113, a first temperature sensor 2 for detecting the temperature of the nucleic acid lysis zone 111, a second temperature sensor 3 for detecting the temperature of the nucleic acid washing zone 112, and a third temperature sensor for detecting the temperature of the nucleic acid amplification detection zone 113 are respectively provided.

[0021] In this embodiment, the temperature probe 1 includes a frustum-shaped tube 11 with a diameter that gradually decreases from top to bottom, a cover 12 detachably installed on the top of the frustum-shaped tube 11, and two insertion tubes 13 extending downward from the bottom of the frustum-shaped tube 11. The frustum-shaped tube 11 has a hollow cavity. The nucleic acid lysis zone 111 is located in the upper part of the frustum-shaped tube 11, the nucleic acid washing zone 112 is located in the lower part of the frustum-shaped tube 11, and the nucleic acid amplification detection zone 113 is located at the two insertion tubes 13.

[0022] The first temperature sensor 2 is located at the center of the upper part of the frustum-shaped tube 11, the second temperature sensor 3 is located at the center of the lower part of the frustum-shaped tube 11, and there are two third temperature sensors, which are respectively located on the upper side of the two insertion tubes 13. The cover 12 is provided with a circuit board connected to the first temperature sensor 2, the second temperature sensor 3 and the third temperature sensor.

[0023] In this embodiment, a first metal point 5 is provided on the outer wall of the temperature probe corresponding to the nucleic acid lysis region 111 ring, and the first metal point 5 is connected to the first temperature sensor 2. A second metal point 6 is provided on the outer wall of the temperature probe corresponding to the nucleic acid lysis region 111 ring, and the second metal point 6 is connected to the second temperature sensor 3. A metal probe 7 extending outward is provided inside the cannula 13, and the metal probe 7 is connected to the third temperature sensor.

[0024] In this embodiment, both the first temperature sensor 2 and the second temperature sensor 3 are provided with long metal wires 8 that can be rolled up to ensure that the first temperature sensor 2 and the second temperature sensor 3 are located at the center of the nucleic acid lysis region 111 and the nucleic acid washing region 112, respectively.

[0025] In this embodiment, the nucleic acid lysis region 111, the nucleic acid washing region 112, and the nucleic acid amplification and detection region 113 are respectively provided with a temperature measuring medium, which is air, pure water, or gel.

[0026] In this embodiment, the first temperature sensor 2, the second temperature sensor 3, and the third temperature sensor are all platinum resistance sensors.

[0027] In this embodiment, there are several temperature probes 1. The rapid nucleic acid detection instrument calibration device also includes a controller. The several temperature probes 1 are all connected to the controller. The controller includes a housing. The housing contains a control circuit board, a power module, a communication module, and a wireless communication module. The power module, the communication module, and the wireless communication module are all connected to the control circuit board. The housing has several interfaces connected to the control circuit board for plugging in the temperature probes 1.

[0028] This invention incorporates three different temperature sensors within the same temperature probe 1, corresponding to different detection zones of the detector, to effectively detect temperature field changes in different areas. Furthermore, by using a long metal wire 8, the sensors can be adjusted to the center of the temperature measurement zone, thus ensuring measurement accuracy. The selection of the sensors, between platinum resistance thermometers and thermocouples, prioritizes platinum resistance thermometers, ensuring they accurately and quickly reflect temperature changes in the rapid nucleic acid detector, thereby guaranteeing calibration accuracy and ensuring the normal operation of the nucleic acid detector.

[0029] The above description is merely an embodiment of this utility model and does not limit the scope of patent protection of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the scope of patent protection of this utility model.

Claims

1. A rapid nucleic acid detector calibration device, characterized in that: The device includes a temperature probe, which contains, from top to bottom, a nucleic acid lysis zone, a nucleic acid washing zone, and a nucleic acid amplification detection zone corresponding to the nucleic acid detector. At the center of each of the nucleic acid lysis zone, nucleic acid washing zone, and nucleic acid amplification detection zone, there is a first temperature sensor for detecting the temperature of the nucleic acid lysis zone, a second temperature sensor for detecting the temperature of the nucleic acid washing zone, and a third temperature sensor for detecting the temperature of the nucleic acid amplification detection zone.

2. The rapid nucleic acid detection instrument calibration device according to claim 1, characterized in that: The temperature probe includes a frustum-shaped tube with a diameter that gradually decreases from top to bottom, a cover that can be detachably installed on the top of the frustum-shaped tube, and two insertion tubes extending downward from the bottom of the frustum-shaped tube. The nucleic acid lysis zone is located in the upper part of the frustum-shaped tube, the nucleic acid washing zone is located in the lower part of the frustum-shaped tube, and the nucleic acid amplification detection zone is located at the two insertion tubes. The first temperature sensor is located at the center of the upper part of the frustum-shaped tube, the second temperature sensor is located at the center of the lower part of the frustum-shaped tube, and there are two third temperature sensors, which are respectively located on the upper side of the two insertion tubes. The cover body is provided with a circuit board connected to the first temperature sensor, the second temperature sensor and the third temperature sensor.

3. The rapid nucleic acid detector calibration device according to claim 2, characterized in that: The outer wall of the temperature probe is provided with a first metal point corresponding to the nucleic acid lysis region, and the first metal point is connected to the first temperature sensor. The outer wall of the temperature probe is provided with a second metal point corresponding to the nucleic acid lysis region, and the second metal point is connected to the second temperature sensor. The cannula is provided with an outwardly protruding metal probe, and the metal probe is connected to the third temperature sensor.

4. The rapid nucleic acid detector calibration device according to claim 2, characterized in that: Both the first and second temperature sensors are provided with long metal wires that can be rolled up to ensure that the first and second temperature sensors are located at the center of the nucleic acid lysis region and the nucleic acid washing region, respectively.

5. The rapid nucleic acid detector calibration device according to claim 1, characterized in that: The nucleic acid lysis zone, nucleic acid washing zone, and nucleic acid amplification and detection zone are each equipped with a temperature measuring medium, which can be air, pure water, or gel.

6. The rapid nucleic acid detection instrument calibration device according to claim 1, characterized in that: The first temperature sensor, the second temperature sensor, and the third temperature sensor are all platinum resistance sensors.

7. The rapid nucleic acid testing instrument calibration device of claim 1, wherein: The device includes several temperature probes. The rapid nucleic acid detection instrument calibration device also includes a controller. The temperature probes are all connected to the controller. The controller includes a housing. Inside the housing are a control circuit board, a power module, a communication module, and a wireless communication module. The power module, communication module, and wireless communication module are all connected to the control circuit board. The housing has several interfaces connected to the control circuit board for inserting temperature probes.

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