A fluid replenishment device for cell culture
By introducing ultraviolet light sterilization and an automated stirring rod into the cell culture replenishment device, the problem of difficult cleaning of the device in the prior art has been solved, achieving aseptic replenishment and simplified operation, and ensuring a clean culture environment.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- JIANGSU HILLGENE BIOPHARMA CO LTD
- Filing Date
- 2025-06-27
- Publication Date
- 2026-07-03
AI Technical Summary
Existing cell culture fluid replenishment devices are difficult to clean effectively after use, easily introducing contaminants, and are cumbersome to operate.
A liquid replenishment device comprising a clean bench and a tank was designed, equipped with an ultraviolet lamp and an automatic stirring rod. The device achieves aseptic replenishment of culture medium through ultraviolet sterilization and automatic stirring rod, and the design of a separately openable door panel prevents dust from entering.
It enables aseptic replenishment of culture medium, avoids contamination by other microorganisms, simplifies the operation process, and ensures a clean culture environment.
Smart Images
Figure CN224450711U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell culture technology, specifically to a fluid replenishment device for cell culture. Background Technology
[0002] Cell culture involves adding a suitable culture medium to a culture dish 15. After the heated culture medium solidifies, it facilitates the formation of a solidified state in the culture dish 15. After inoculating the culture, the culture dish is sealed and placed in a constant temperature incubator. The culture will be established after a few days of standing. If it is necessary to add culture medium midway, a liquid replenishment device is required.
[0003] The liquid replenishment device mainly consists of a storage structure and a liquid spraying mechanism. After opening the petri dish, the liquid can be dripped in to replenish the culture medium. However, such devices need to be disassembled and cleaned separately after use, which is very troublesome. If the disinfection is not complete, it is difficult to ensure the cleanliness of the culture medium and it is easy to generate contaminants.
[0004] Now, a novel fluid replenishment device for cell culture is proposed to solve the above problems. Utility Model Content
[0005] The purpose of this invention is to provide a fluid replenishment device for cell culture, so as to solve the problem of lack of internal cleaning mechanism mentioned in the background art.
[0006] To achieve the above objectives, this utility model provides the following technical solution: a cell culture fluid replenishment device, comprising a laminar flow hood and a tank. The tank is fixed to the top of the laminar flow hood, and a sleeve is movably connected to the top of the tank. A protective sleeve is fixed to the left side of the sleeve. A second ultraviolet lamp is installed in the upper left corner of the tank. A frame is fixed to the center of the top of the tank, and a motor is fixed to the top of the frame. A dial is movably connected between the frame and the tank. A stirring rod is movably connected between the dial and the sleeve. A plug is fixedly inserted into the upper right corner of the tank. A liquid pump is fixed to the center of the top of the laminar flow hood. A second ultraviolet lamp is fixed to each side of the top of the laminar flow hood.
[0007] Preferably, the bottom of the output shaft of the motor is fixedly connected to the stirring rod, and the sleeve rotates horizontally with the dial wheel.
[0008] Preferably, a column is fixed at the center of the bottom of the ultra-clean workbench, and a sleeve is movably sleeved on the outside of the column. A positioning bolt is threaded to the lower front end of the sleeve. A hole is provided between the upper and lower ends of the front end of the column. A motor is fixed at the upper part of the inside of the sleeve. A platform body is fixed at the top of the sleeve. Cylinders are installed on both sides of the outside of the platform body. A clamping block is fixed to the side of the top of the piston rod of the cylinder. A limit rod is horizontally fixed at the center of the inside of the platform body. A petri dish is placed on the surface of the platform body.
[0009] Preferably, the piston rod of the cylinder is sleeved on both sides of the limiting rod, and the top of the output end of the second motor is fixedly connected to the platform.
[0010] Preferably, a door panel is movably hinged to the left side of the front end of the clean bench, and a frame is fixed at the center of the door panel. A through groove is provided at the center of the top of the frame, and a baffle is movably connected in the through groove. Screw holes are provided between the top and bottom of both sides of the baffle. Two sets of fastening bolts are threadedly connected to the upper part of the center of the door panel. A locking block one is fixed to the right side of the front end of the clean bench, and a locking block two is fixed to the left side of the front end of the door panel.
[0011] Preferably, the baffle slides up and down along the inner wall of the frame, and the fastening bolt passes through the door panel and the screw hole.
[0012] Compared with the prior art, the beneficial effects of this utility model are: this cell culture replenishment device not only prevents the generation of miscellaneous bacteria and adjusts the liquid injection position of the culture dish, but also avoids the device from sucking in excess dust;
[0013] The system consists of two UV lamps fixed to the top of the clean bench on both sides and a UV lamp installed in the upper left corner of the tank. The tank contains sterilized culture medium. The culture medium is guided by a pump through a rotating stirring rod to be injected into a petri dish on the bench. After the device is used, the inner wall is cleaned first, then the door is closed and UV lamp 1 is turned on to disinfect the clean bench. The tank is then sterilized by UV lamp 2. After the sterilization is completed, the dial is turned so that the protective cover is attached to the surface of UV lamp 2 for protection, which can prevent the injection device from being contaminated with bacteria.
[0014] By fixing a platform to the top of the sleeve, the sleeve is moved longitudinally along the column, aligned with the hole, and then the positioning bolt is rotated for reinforcement. The opened culture dish is placed on the platform. The cylinders on both sides adjust the position of the clamping block along the limit rod through the piston rod, and then adjust the position of the culture dish on the upper surface of the platform to facilitate the liquid pump to replenish the liquid to the culture dish. After completion, the culture dish is rotated by the motor to make the liquid flow evenly. There is no need to manually touch the culture dish, which ensures the cleanliness of the liquid replenishment operation.
[0015] The door panel is hinged to the left side of the front end of the clean bench. The baffle is pulled along the longitudinal groove, and the bolts are tightened after aligning with the screw holes on both sides. The chamber can be opened from the center of the door panel to put in or take out the petri dish without opening the door panel as a whole. The door panel and the clean bench are respectively equipped with a second locking block and a first locking block to prevent the door panel from opening automatically and to block dust from entering the shell. Attached Figure Description
[0016] Figure 1 This is a frontal cross-sectional view of the present invention.
[0017] Figure 2 This is a front view cross-sectional structural diagram of the tank body of this utility model;
[0018] Figure 3 This is a front view cross-sectional structural diagram of the platform of this utility model;
[0019] Figure 4 This is a front view structural diagram of the door panel of this utility model.
[0020] In the diagram: 1. Clean bench; 2. UV lamp one; 3. Tank; 4. Protective sleeve; 5. UV lamp two; 6. Frame; 7. Motor one; 8. Dial wheel; 9. Sleeve; 10. Stopper; 11. Stirring rod; 12. Liquid pump; 13. Clamping block one; 14. Platform; 15. Petri dish; 16. Door panel; 17. Frame; 18. Clamping block two; 19. Through groove; 20. Baffle; 21. Screw hole; 22. Fastening bolt; 23. Cylinder; 24. Limiting rod; 25. Sleeve; 26. Positioning bolt; 27. Column; 28. Hole; 29. Motor two; 30. Clamping block. Detailed Implementation
[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0022] Please see Figure 1-4 An embodiment of this utility model provides a cell culture fluid replenishment device, including a clean bench 1 and a tank 3. The tank 3 is fixed to the top of the outside of the clean bench 1, and a sleeve 9 is movably connected to the top of the inside of the tank 3. A protective sleeve 4 is fixed to the left side of the outside of the sleeve 9. A second ultraviolet lamp 5 is installed in the upper left corner inside the tank 3. A frame 6 is fixed to the center of the top of the outside of the tank 3, and a motor 7 is fixed to the top of the frame 6. A dial 8 is movably connected between the frame 6 and the tank 3. A stirring rod 11 is movably connected between the dial 8 and the sleeve 9. A plug 10 is fixedly inserted into the upper right corner of the tank 3. A liquid pump 12 is fixed to the center of the top of the inside of the clean bench 1. A second ultraviolet lamp 2 is fixed to both sides of the top of the inside of the clean bench 1.
[0023] The bottom of the output shaft of motor 7 is fixedly connected to the stirring rod 11, and the sleeve 9 rotates horizontally with the dial wheel 8;
[0024] Specifically, such as Figure 1 and Figure 2As shown, the tank 3 stores the sterilized culture medium. The culture medium is guided by the rotating stirring rod 11 of the motor 7 through the liquid pump 12 into the petri dish 15 on the platform 14. After the device is used, the inner wall is cleaned first, then the door panel 16 is closed and the ultraviolet lamp 2 is turned on to disinfect the ultra-clean workbench 1. The tank 3 is then sterilized by the ultraviolet lamp 5 to prevent the device from growing miscellaneous bacteria.
[0025] A column 27 is fixed at the center of the bottom of the clean bench 1, and a sleeve 25 is movably sleeved on the outside of the column 27. A positioning bolt 26 is threaded to the lower front end of the sleeve 25. A hole 28 is provided between the upper and lower ends of the front end of the column 27. A motor 29 is fixed at the upper inside of the sleeve 25. A platform 14 is fixed at the top of the sleeve 25. Cylinders 23 are installed on both sides of the outside of the platform 14. A clamping block 30 is fixed on the side of the top of the piston rod of the cylinder 23. A limit rod 24 is horizontally fixed at the center inside the platform 14. A petri dish 15 is placed on the surface of the platform 14. The piston rods of the cylinders 23 are sleeved on both sides of the limit rod 24. The top of the output end of the motor 29 is fixedly connected to the platform 14.
[0026] Specifically, such as Figure 1 and Figure 3 As shown, the sleeve 25 is moved longitudinally along the column 27, aligned with the hole 28, and then the positioning bolt 26 is rotated to reinforce it. The opened culture dish 15 is placed on the platform 14. The cylinders 23 on both sides adjust the position of the clamping block 30 along the limit rod 24 through the piston rod, and then adjust the position of the culture dish 15 on the upper surface of the platform 14 to facilitate the liquid pump 12 to replenish the liquid to the culture dish 15. After the operation is completed, the motor 29 rotates the culture dish 15 to make the liquid flow evenly.
[0027] A door panel 16 is movably hinged to the left side of the front end of the clean bench 1, and a frame 17 is fixed at the center of the interior of the door panel 16. A through groove 19 is provided at the center of the top of the frame 17, and a baffle 20 is movably connected in the through groove 19. Screw holes 21 are provided between the upper and lower sides of the baffle 20. Two sets of fastening bolts 22 are threadedly connected to the upper part of the center of the door panel 16. A locking block 13 is fixed to the right side of the front end of the clean bench 1, and a locking block 28 is fixed to the left side of the front end of the door panel 16. The baffle 20 slides up and down along the inner wall of the frame 17, and the fastening bolts 22 pass through the door panel 16 and the screw holes 21.
[0028] Specifically, such as Figure 1 and Figure 4 As shown, the baffle 20 is pulled longitudinally along the through groove 19, and after aligning with the screw holes 21 on both sides, the fastening bolts 22 are rotated to reinforce it. The chamber can be opened separately from the center of the door panel 16 to put in or take out the culture dish 15 without having to open the door panel 16 as a whole. Furthermore, the door panel 16 and the ultra-clean workbench 1 are respectively equipped with a second locking block 18 and a first locking block 13, which can prevent the door panel 16 from opening automatically.
[0029] Working principle: In use, the baffle 20 is first pulled along the longitudinal direction of the through groove 19, aligned with the screw holes 21 on both sides, and then the fastening bolts 22 are rotated to tighten. The chamber can be opened from the center of the door panel 16 to insert or remove the petri dish 15 without opening the entire door panel 16. The door panel 16 and the ultra-clean workbench 1 are respectively equipped with locking blocks 18 and 13 to prevent the door panel 16 from opening automatically. Then, the sleeve 25 is moved along the longitudinal direction of the column 27, aligned with the hole 28, and the positioning bolts 26 are rotated to tighten. The opened petri dish 15 is then placed on the platform 14. The cylinders 23 on both sides are adjusted along the limit rods 24 via piston rods. The position of the clamping block 30 is adjusted, and then the position of the culture dish 15 is adjusted on the upper surface of the platform 14 to facilitate the liquid pump 12 to replenish the liquid to the culture dish 15. After that, the culture dish 15 is rotated by the motor 29 to make the liquid flow evenly. The tank 3 stores the sterilized culture medium. The culture medium is guided through the liquid pump 12 to the culture dish 15 on the platform 14 by rotating the stirring rod 11 by the motor 7. After the device is used, the inner wall is cleaned first, then the door panel 16 is closed and the ultraviolet lamp 2 is turned on to disinfect the ultra-clean workbench 1. The tank 3 is then sterilized by the ultraviolet lamp 5. After that, the dial wheel 8 is turned so that the protective cover 4 is put on the surface of the ultraviolet lamp 5 for protection.
[0030] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.
Claims
1. A cell culture supplementing device comprising an ultra-clean workbench (1) and a tank body (3), characterized in that: The top of the clean bench (1) is fixed with a tank (3), and the top of the inside of the tank (3) is movably connected with a sleeve (9). A protective sleeve (4) is fixed on the left side of the outside of the sleeve (9). A second ultraviolet lamp (5) is installed in the upper left corner of the inside of the tank (3). A frame (6) is fixed at the center of the top of the outside of the tank (3), and a motor (7) is fixed at the top of the frame (6). A dial wheel (8) is movably connected between the frame (6) and the tank (3). A stirring rod (11) is movably connected between the dial wheel (8) and the sleeve (9). A plug (10) is fixedly inserted into the upper right corner of the tank (3). A liquid pump (12) is fixed at the center of the top of the inside of the clean bench (1). A first ultraviolet lamp (2) is fixed on both sides of the top of the inside of the clean bench (1).
2. The medium supplementing device for cell culture according to claim 1, wherein: The bottom of the output shaft of the motor (7) is fixedly connected to the stirring rod (11), and the sleeve (9) rotates horizontally with the dial wheel (8).
3. The medium supplementing device for cell culture according to claim 1, wherein: A column (27) is fixed at the center of the bottom of the ultra-clean workbench (1), and a sleeve (25) is movably sleeved on the outside of the column (27). A positioning bolt (26) is threaded to the lower front end of the sleeve (25). A hole (28) is provided between the upper and lower front ends of the column (27). A motor (29) is fixed at the upper inside of the sleeve (25). A platform (14) is fixed at the top of the sleeve (25), and cylinders (23) are installed on both sides of the outside of the platform (14). A clamping block (30) is fixed on the side of the piston rod top of the cylinder (23). A limit rod (24) is fixed horizontally at the center inside the platform (14). A petri dish (15) is placed on the surface of the platform (14).
4. The medium supplementing device for cell culture according to claim 3, wherein: The piston rod of the cylinder (23) is respectively sleeved on both sides of the limiting rod (24), and the top of the output end of the second motor (29) is fixedly connected to the platform (14).
5. The medium supplementing device for cell culture according to claim 1, wherein: A door panel (16) is movably hinged to the left side of the front end of the ultra-clean workbench (1), and a frame (17) is fixed at the center inside the door panel (16). A through groove (19) is provided at the center of the top of the frame (17), and a baffle (20) is movably connected in the through groove (19). Screw holes (21) are provided between the top and bottom of the two sides of the baffle (20). Two sets of fastening bolts (22) are threadedly connected to the top of the center of the door panel (16). A locking block one (13) is fixed to the right side of the front end of the ultra-clean workbench (1), and a locking block two (18) is fixed to the left side of the front end of the door panel (16).
6. The device of claim 5, wherein: The baffle (20) slides up and down along the inner wall of the frame (17), and the fastening bolt (22) passes through the door panel (16) and the screw hole (21).