An efficient micropropagation system for improved propagation of strawberry plants

Abstract

A micropropagation system (100) for improved propagation of strawberry plants, comprising: a surface sterilization chamber (102) comprising one or more ultraviolet lamps (102e) for sterilizing strawberry runner tip explants; a sterile culture production chamber (104) for culturing the sterilized explants in a controlled culture room in which a temperature of 22°C to 23°C and a photoperiod of 12 hours per day is maintained; a regeneration chamber (106) for a plurality of shoots for inducing shoot regeneration in the explants; a root formation chamber (108) for inducing root formation in the regenerated shoots; and an acclimatization chamber (110) for acclimatizing the rooted plantlets to ex vitro conditions.

Description

Field of the invention:

[0001] The present invention relates to plant biotechnology. More specifically, the present invention relates to an efficient micropropagation system that promotes the propagation of strawberry plants through the use of tissue culture. Background of the invention:

[0002] Plant propagation is promoted using various methods, such as tissue culture. Tissue culture is crucial for advancing strawberry production and research because it enables rapid and efficient plant propagation.

[0003] Traditional methods of propagating strawberry plants (Fragaria × ananassa) Strawberry production is primarily vegetative propagation by runners, resulting in a limited number of seedlings. In addition, these methods expose the seedlings to various diseases, such as fungal diseases, Strawberry Mottle Virus (SMoV), and Strawberry Mild Yellow Edge Virus (SMYEV), which pose a significant threat to strawberry production.

[0004] To overcome these challenges, a micropropagation system for improved propagation of strawberry plants needs to be developed that utilizes tissue culture techniques to produce numerous disease-free, genetically identical plants within a short period of time, thus ensuring a constant supply of high-quality planting material.

[0005] The technical advances disclosed by the present invention overcome the limitations and disadvantages of existing and conventional systems and methods. Summary of the invention:

[0006] The present invention relates to a micropropagation system that focuses on promoting the propagation of strawberry plants through the use of tissue culture.

[0007] An object of the present invention is to promote the propagation of strawberry plants.

[0008] Another object of the present invention is to provide a system for strawberry cultivation that will improve the quality of seedlings and ensure a high survival rate, ultimately leading to a higher strawberry yield.

[0009] Another object of the present invention is to contribute to the development of skills and dissemination of scientific knowledge to produce strawberries on a mass scale.

[0010] Another object of the present invention is the use of the growth hormone metatopolin for shoot propagation, which regenerates more than hundreds of plants from one explant, and

[0011] Another object of the present invention is to provide a rapid and cost-effective micropropagation or tissue culture system for strawberry plants.

[0012] The present invention describes a micropropagation system for improved propagation of strawberry plants, comprising: a surface sterilization chamber with one or more ultraviolet lamps for sterilizing the runner tips of strawberries; a sterile culture production chamber for cultivating the sterilized explants in a controlled culture room maintaining a temperature of 22-23°C and a photoperiod of 12 hours per day; a shoot regeneration chamber for inducing shoot regeneration in the explants; a root initiation chamber for inducing root formation in the regenerated shoots; and an acclimatization chamber for acclimating the rooted plantlets to ex vitro conditions.

[0013] To further clarify the advantages and features of the present invention, the invention will be described in more detail with reference to specific embodiments illustrated in the accompanying drawings. These drawings illustrate only typical embodiments of the invention and therefore do not represent a limitation of its scope. The invention will be described and explained in more detail with reference to the accompanying drawings. Short description of the figures:

[0014] These and other features, aspects, and advantages of the present invention will become more readily understood when the following detailed description is read with reference to the accompanying drawings, in which like characters represent like parts throughout. Fig. Figure 1 shows a block diagram of a micropropagation system for improved propagation of strawberry plants. Fig. Figure 2 shows a block diagram of a surface sterilization chamber. Fig. Figure 3 shows a block diagram of the acclimatization chamber for acclimatizing the rooted plantlets to ex vitro conditions.

[0015] Those skilled in the art will also appreciate that the elements in the drawings are shown for convenience and are not necessarily to scale. For example, the flowcharts illustrate the method by key steps to enhance understanding of aspects of the present disclosure. Furthermore, with respect to device construction, one or more components of the device may be represented in the drawings by conventional symbols. The drawings may show only the specific details relevant to understanding embodiments of the present disclosure in order not to clutter the drawings with details that would be readily apparent to those skilled in the art from the present description. Detailed description:

[0016] To facilitate understanding of the principles of the invention, reference will now be made to the embodiment illustrated in the drawings and will be clearly described. However, the scope of the invention is not limited thereby. Changes and further modifications to the illustrated system, as well as further applications of the principles of the invention, are possible, as would normally occur to one skilled in the art to which the invention pertains.

[0017] It will be understood by those skilled in the art that the foregoing general description and the following detailed description are exemplary and explanatory of the invention and are not intended to be limiting thereof.

[0018] References in this specification to "one aspect," "another aspect," or similar expressions mean that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Therefore, the terms "in one embodiment," "in another embodiment," and similar expressions in this specification may or may not refer to the same embodiment.

[0019] The terms "comprises," "comprising," or other variations thereof are intended to cover non-exclusive inclusion, such that a process or method comprising a list of steps may include not only those steps, but also additional steps not expressly listed or inherent in that process or method. Likewise, the statement "comprises" for one or more devices, subsystems, elements, structures, or components does not exclude, without further limitation, the existence of other devices, subsystems, elements, structures, components, or additional devices, subsystems, elements, structures, or components.

[0020] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains. The systems, methods, and examples provided herein are for illustrative purposes only and should not be considered limiting.

[0021] Embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0022] Fig. 1 shows a block diagram of a micropropagation system (100) for improved propagation of strawberry plants, comprising: a surface sterilization chamber (102), a sterile culture production chamber (104), a shoot regeneration chamber (106), a root formation chamber (108), and an acclimation chamber (110).

[0023] The surface sterilization chamber (102) comprises one or more ultraviolet lamps (102e) for sterilizing strawberry runner tip explants.

[0024] The sterile culture production chamber (104) is used to cultivate the sterilized explants in a controlled culture room in which a temperature of 22 °C to 23 °C and a photoperiod of 12 hours per day is maintained.

[0025] The multiple shoot regeneration chamber (106) for inducing shoot regeneration in the explants.

[0026] The root formation chamber (108) serves to stimulate root formation in the regenerated shoots.

[0027] The acclimatization chamber (110) is used to acclimatize the rooted plants to ex vitro conditions.

[0028] Fig. Figure 2 shows a block diagram of a surface sterilization chamber (102). The chamber (102) comprises: a collection device (102a), a cutting and washing device (102b), a sterilization unit (102c), a laminar airflow chamber (102d), a UV sterilization device (102e), a treatment unit (102f), and a washing and storage unit (102g).

[0029] The collection device (102a) is configured to collect strawberry runner tips from plants protected under controlled greenhouse conditions and maintain hydration using distilled water.

[0030] The cutting and washing device (102b) is configured to segment the runner tips into smaller pieces and wash them under tap or RO water with a muslin cloth in multiple washing cycles.

[0031] The sterilization unit (102c) is configured to transfer the explants into sterilized distilled water and place them in a laminar airflow chamber (102d).

[0032] The UV sterilizer (102e) is configured to sterilize the laminar hood with UV rays for 20-30 minutes before starting a sterilization process.

[0033] The treatment unit (102f) is configured to transfer explants into a 0.1% mercuric chloride solution in the laminar hood for 10 minutes.

[0034] The washing and storage unit (102 g) is configured to rinse the explants several times with sterilized distilled water and store them in sterilized distilled water until inoculation.

[0035] In one embodiment, the regeneration of multiple shoots, the five different concentrations of metatopolin used for the regeneration of multiple shoots range from 0.5 mg / L to 3 mg / L, wherein the culture medium comprises MS medium with a sucrose concentration of 3% and an agar concentration of 0.8%, and for root formation, the culture medium for root formation is prepared with ½ MS medium, 3% sucrose, 0.6% agar, and a pH of 5.8. Fig.Figure 3 shows a block diagram of the acclimatization chamber (110) for acclimatizing the rooted plantlets to ex vitro conditions. The acclimatization chamber (110) is used to acclimatize strawberry plantlets and comprises: a root development chamber (110a) for growing the roots to a length of 8 to 9 cm; a temperature control unit (110b) for transferring the rooted plantlets into culture bottles at room temperature and opening the lids for 24 hours; a washing unit (110c) for cleaning the plantlets removed from the culture medium; a potting unit (110d) for transferring the plantlets into pots filled with vermiculite growth medium; a covering device (110e) with transparent polyethylene bags for tightly covering the new plantlets for three to four days and then loosely covering them for more than four days to protect them from air exchange. an irrigation system (110f) with sprayers to moisten the plants;a growth monitoring system (110 g) for observing the growth of the plantlets in terms of height and number of leaves, a transfer mechanism for transferring the plantlets into soil after 15 to 20 days of growth in the growth medium, and a controlled growth chamber (110 h) with a temperature control system for maintaining a temperature range of 22 °C to 23 °C and a lighting system for providing a photoperiod of 12 hours of light per day.;

[0036] In one embodiment, the surface sterilization chamber (102) comprises a rotating platform designed to uniformly expose the strawberry runner tip explants to the ultraviolet lamps (102e), thus ensuring consistent sterilization across all surfaces of the explants. Furthermore, the surface sterilization chamber (102) comprises a rotating UV lamp assembly mounted on an automated rail system, allowing the UV lamps to move laterally across the chamber to ensure uniform exposure of the strawberry runner tip explants to varying UV intensities, thus improving the thoroughness of the sterilization process.In addition, the surface sterilization chamber (102) is equipped with a two-phase airflow system that first directs a laminar airflow over the explants to remove surface deposits, followed by a targeted ionized airflow that neutralizes any remaining contaminants before the UV sterilization process begins.

[0037] In one embodiment, the sterile culture production chamber (104) comprises a temperature control unit with precision thermal sensors evenly distributed throughout the chamber, ensuring that the temperature remains within the range of 22°C to 23°C with a deviation of no more than ±0.1°C. Furthermore, the sterile culture production chamber (104) contains a dynamic humidity control system with a network of microhygrometers distributed throughout the chamber, each connected to a localized ultrasonic humidifier, enabling zone-specific humidity adjustments to maintain optimal humidity levels.In addition, the sterile culture production chamber (104) is equipped with a programmable environmental control unit specifically designed to maintain precise temperature control in the range of 22 °C to 23 °C and a 12-hour photoperiod that can be adjusted according to the requirements of shoot regeneration and root formation.

[0038] In one embodiment, the shoot regeneration chamber (106) comprises a humidity control system with integrated mist nozzles designed to maintain an optimal humidity level by evenly distributing the humidity within the chamber. Furthermore, the shoot regeneration chamber (106) is equipped with an automated explant rotation mechanism that regularly rotates the explants at precise angles, ensuring consistent light exposure and nutrient uptake from all directions, thereby promoting uniform and accelerated shoot development. Furthermore, the shoot regeneration chamber (106) comprises an integrated electromagnetic pulse emitter that intermittently emits low-frequency electromagnetic pulses to stimulate cell differentiation in the explants, thereby improving the efficiency of shoot regeneration.In addition, the shoot regeneration chamber (106) includes a nutrient delivery system configured to deliver kinetin and metatopolin and is integrated with a monitoring system that tracks shoot length and automatically adjusts nutrient supply and environmental conditions to optimize shoot growth within a period of 15 to 20 days.

[0039] In one embodiment, the root formation chamber (108) comprises an adjustable light panel that allows precise control of the light intensity and spectrum and is specifically tuned to wavelengths that promote root development in the regenerated shoots. Furthermore, the root formation chamber (108) comprises a micro-oxygenation system that injects controlled microbubbles of oxygen into the culture medium surrounding the roots, thus promoting improved root growth by optimizing oxygen availability in the root zone.

[0040] In one embodiment, the acclimatization chamber (110) is equipped with a ventilation system including adjustable air inlets and outlets designed to control air exchange and maintain the desired CO2 concentration for optimal acclimatization of the young plants. Furthermore, the acclimatization chamber (110) is equipped with a differential pressure air filtration system that maintains a positive pressure environment to prevent external contaminants from entering the chamber while allowing a controlled exchange of CO2 and O2 to optimize acclimatization.

[0041] In one embodiment, the surface sterilization chamber (102) includes an integrated optical particle counter that continuously monitors the air quality within the chamber and triggers an automatic UV sterilization cycle when the particle concentration exceeds a predefined threshold. This ensures a contamination-free environment for the explants. Furthermore, the surface sterilization chamber (102) includes a real-time contamination detection system with a laser-induced fluorescence sensor array that detects the presence of biological contaminants on the explants and automatically adjusts the UV sterilization parameters to ensure complete decontamination.

[0042] The present invention relates to a micropropagation system for the improved propagation of strawberry plants. The system enables the production of many disease-free and genetically identical plants in a short period of time, thus ensuring a constant supply of high-quality planting material. The system enables improved propagation of strawberry plants by utilizing the plant growth hormone metatopolin to generate multiple shoots. From one callus, 70 to 8 shoots can be generated. By using the same plant hormone, the system thus enables the production of both shoots and callus. The micropropagation system improves the quality of the seedlings, ensures a high survival rate regardless of the season, and thus increases the strawberry yield.By improving the propagation of strawberry plants, the system offers a promising solution to the challenges of traditional propagation methods and a reliable and efficient approach for the large-scale production of disease-free and high-quality strawberry plants.

[0043] The system comprises several components that perform functions such as surface sterilization of strawberry explants, sterile culture initiation, shoot regeneration with kinetin, multiple shoot regeneration with metatopolin, callus formation, root formation, and acclimatization. To ensure the effective function of the micropropagation system, careful attention is paid to media composition, hormone concentrations, and controlled environmental conditions.

[0044] In an exemplary embodiment, the system includes a surface sterilization chamber with one or more UV lamps for sterilizing strawberry runner tips. The surface sterilization chamber is configured for surface sterilization.The surface sterilization chamber comprises: a collection device for collecting strawberry runner tips from plants maintained in a controlled greenhouse environment, where the runner tips are immediately placed in distilled water to maintain hydration; a cutting and washing device that segments the collected runner tips into smaller explant pieces and washes them under tap or RO water with a muslin cloth, with four to five washing cycles carried out at short intervals; a sterilization unit for transferring the washed explants into sterilized distilled water and placing them in a laminar airflow chamber; a UV sterilization device that sterilizes the laminar airflow hood by emitting UV rays for a duration of 20 to 30 minutes before initiating further sterilization processes; a treatment unit that places the explants in a 0.1 m² container for 10 minutes.1% mercuric chloride solution in the sterilized laminar airflow chamber; and a washing and storage unit configured to rinse the explants five to six times with sterilized distilled water and store them in sterilized distilled water until further inoculation procedures.

[0045] In an exemplary embodiment, the system comprises a sterile culture production chamber that cultivates the sterilized strawberry explants under precisely controlled environmental conditions. The chamber includes: a culture incubation unit that receives sterilized explants and transfers them to Murashige and Skoog (MS) culture media for an initial incubation period of 7 to 8 days; a contamination monitoring system that checks the explants for microbial infections during the incubation period.Only those explants that remain infection-free and show initial signs of growth are selected for further processing; a temperature control unit with precise temperature sensors distributed throughout the chamber maintains a constant temperature within the range of 22°C to 23°C with a maximum deviation of ±0.1°C; and a photoperiod regulation system that ensures a constant 12-hour light and 12-hour dark cycle per day to enable optimal explant development during the culture production phase. All cultivation procedures are carried out under aseptic conditions in the controlled environment of the sterile culture production chamber.

[0046] In an exemplary embodiment, the system comprises a multi-shoot regeneration chamber that induces both shoot regeneration and multi-shoot regeneration in strawberry runner tip explants. The chamber has integrated environmental control and nutrient delivery systems and is configured to perform shoot regeneration and multi-shoot regeneration as follows: For shoot regeneration, the system includes a media preparation unit. This unit prepares culture media by adding 0.5 mg / L of the plant growth hormone kinetin, MS medium, 30 g / L sucrose, and 8 g / L agar to distilled water, adjusting the pH to 5.8. After 3 to 4 days of media preparation, selected explants are inoculated into the culture medium. After 15 to 20 days, the inoculation response becomes apparent, with shoot lengths of 3-5 cm. The system includes a controlled culture chamber with a climate control unit that maintains the temperature between 22°C and 23°C and ensures a photoperiod of 12 hours of light per day.

[0047] For multi-shoot regeneration, the multi-shoot chamber allows for the production of multi-shoot regeneration culture media using the plant growth hormone metatopolin. Five different concentrations of metatopolin are used for multi-shoot regeneration. To prepare the culture medium with a pH of 5.8, 2 mg of metatopolin, MS medium, 30,000 mg of sucrose, and 8,000 mg of agar were added to each liter of distilled water. The best response for multi-shoot regeneration is observed after 15 days of inoculation. At this concentration (MS medium + 2 mg / L metatopolin), the number of shoots is more than 100.

[0048] In an exemplary embodiment, the system comprises a rooting chamber configured to initiate root formation in the regenerated strawberry shoots, wherein the rooting chamber is further configured to: initiate root formation in regenerated shoots once they reach a length of 4 to 5 cm; utilize a root culture medium consisting of 1 / 2 MS medium, 3% sucrose, 0.6% agar, and adjusted to a pH of 5.8 per liter of distilled water, which has been shown to significantly enhance root formation; maintain the root formation process over a period of approximately 20 days to develop a healthy and strong root system; and operate in a controlled culture room environment in which the temperature is maintained in the range of 22°C to 23°C and a photoperiod of 12 hours per day is ensured for optimal root development.

[0049] In an exemplary embodiment, the system comprises an acclimatization chamber configured to acclimatize rooted strawberry plantlets to ex vitro conditions, the acclimatization chamber further configured to transfer plantlets with root lengths of 8 to 9 cm to room temperature by opening the lid of the culture flask for 24 hours, which facilitates gradual adaptation; removing plantlets from the culture medium, washing them thoroughly, and transplanting them into pots containing vermiculite growth medium for further root development; tightly enclosing newly transplanted plantlets in transparent polyethylene bags for three to four days to maintain humidity and reduce transplant shock;After the initial covering period, loosely cover the plantlets for a further four days to ensure gradual air exchange, while continuing to provide humidity with water sprays; monitor the growth of the plantlets by observing their height and number of leaves, and, upon satisfactory development, remove the polyethylene bags and water as needed; transplant the plantlets into soil for final acclimatization after 15 to 20 days of growth in vermiculite medium; and carry out all acclimatization procedures in a controlled growth chamber maintaining a temperature between 22°C and 23°C and a photoperiod of 12 hours of light per day to simulate natural environmental conditions.

[0050] The micropropagation system for improved strawberry plant propagation offers a wide range of industrial applications. It enables the rapid and large-scale propagation of disease-free strawberry seedlings, effectively overcoming the limitations of conventional propagation techniques. Industries involved in commercial strawberry cultivation benefit from a consistent and reliable supply of high-quality, healthy planting material. The system supports farms through higher strawberry yields and improved fruit quality. Because it operates independently of seasonal constraints, the system enables uninterrupted, year-round production of strawberry seedlings and ensures a continuous and predictable supply chain that minimizes dependence on specific growing seasons.Agricultural technology companies can use the system to promote the adoption of advanced tissue culture techniques in agriculture. The system's integrated biosecurity features ensure the production of contamination-free seedlings, thus reducing the risk of widespread plant infections in large-scale cultivation. Research institutions and biotechnology companies can integrate the system into experimental and development-related activities, enabling innovations in seedling propagation efficiency, cost optimization, and variety improvement. The system's production of disease-free young plants ensures compliance with international plant protection standards, thus supporting market expansion worldwide.Furthermore, the system supports ecologically sustainable agricultural practices by significantly reducing the need for chemical treatments commonly used to control disease. This is consistent with the goals of environmentally friendly agriculture and contributes to a lower environmental impact on intensive farms.

[0051] In summary, the industrial applications of strawberry micropropagation range from supporting large-scale commercial cultivation to research and development initiatives, contributing to improved biosecurity, sustainability and overall efficiency of strawberry production processes.

[0052] The system used for strawberry cultivation improves seedling quality and ensures high survival rates, ultimately leading to higher strawberry yields. Transferring this tissue culture technology for strawberry plants to industry can contribute to the development of expertise and dissemination of scientific knowledge for mass strawberry production. recognition 1) PI acknowledges the Odisha State Higher Education Council (OSHEC), Government of Odisha, for funding the Mukhyamantri Research Innovation Project (MRIP-2024) titled “Development of an efficient micropropagation system for improved propagation of strawberry plants by tissue culture method” (Project File No. 24EM / BO / 14). 2) PI also thanks Ravenshaw University, Cuttack, Odisha, for providing the infrastructure and all other necessary facilities to conduct the research.

[0053] The drawings and the foregoing description illustrate examples of embodiments. Those skilled in the art will recognize that one or more of the described elements may well be combined into a single functional element. Alternatively, certain elements may be separated into multiple functional elements. Elements of one embodiment may be added to another embodiment. For example, the order of the processes described herein may be changed and is not limited to the manner described herein. Furthermore, the actions of a flowchart need not be performed in the order shown; nor do all actions need to be performed. Also, actions that are not dependent on other actions may be performed in parallel with the other actions. The scope of the embodiments is in no way limited by these specific examples.Numerous variations, whether explicitly stated in the specification or not, such as differences in structure, dimensions, and use of materials, are possible. The scope of the embodiments is at least as broad as indicated in the following claims.

[0054] Advantages, further benefits, and solutions to problems have been described above with reference to specific embodiments. However, the advantages, advantages, solutions to problems, and any components that may result in or enhance an advantage, advantage, or solution are not to be construed as a critical, required, or essential feature or component of any or all of the claims. REFERENCES 100 A micropropagation system for improved propagation of strawberry plants 102 Surface sterilization chamber 102a Collection device 102b Cutting and washing device 102c Sterilization Unit 102d Laminar air flow chamber 102g Washing and Storage Unit 102e UV sterilizer 102f treatment unit 104 Sterile culture production chamber 106 Multi-shoot regeneration chamber 108 Root formation chamber 110 Acclimatization chamber 110a Root development chamber 110b Temperature control unit 110c washing unit 110d pot unit 110e cover device 110f irrigation system 110g growth monitoring system 110h Controlled growth chamber

Claims

[1] A micropropagation system (100) for improved propagation of strawberry plants, comprising: a surface sterilization chamber (102) comprising one or more ultraviolet lamps (102e) for sterilizing strawberry runner tip explants; a sterile culture production chamber (104) for culturing the sterilized explants in a controlled culture room in which a temperature of 22°C to 23°C and a photoperiod of 12 hours per day is maintained; a regeneration chamber (106) for a plurality of shoots for inducing shoot regeneration in the explants; a root formation chamber (108) for inducing root formation in the regenerated shoots; and an acclimatization chamber (110) for acclimatizing the rooted plantlets to ex vitro conditions. [2] The system of claim 1, wherein the surface sterilization chamber (102) further comprises: a collection device (102a) configured to collect strawberry runner tips from plants protected under controlled greenhouse conditions and to maintain hydration by using distilled water; a cutting and washing device (102b) configured to segment the runner tips into smaller pieces and wash them under tap or RO water with a muslin cloth in multiple washing cycles; a sterilization unit (102c) configured to transfer the explants into sterilized distilled water and place them in a laminar airflow chamber (102d); a UV sterilization device (102e) configured to sterilize the laminar hood with UV rays for 20-30 minutes before initiating a sterilization process; a treatment unit (102f) configured to transfer explants into a 0.1% mercuric chloride solution within the laminar hood for 10 minutes; and a washing and storage unit (102 g) configured to rinse the explants several times with sterilized distilled water and store them in sterilized distilled water until inoculation, and wherein for multi-shoot regeneration, the five different concentrations of metatopolin used for multi-shoot regeneration range from 0.5 mg / l to 3 mg / l, wherein the culture medium comprises MS medium with a sucrose concentration of 3% and an agar concentration of 0.8%, and for root formation, the culture medium for root formation is prepared with ½ MS medium, 3% sucrose, 0.6% agar, and a pH of 5.

8. [3] The system according to claim 1 further comprises acclimatising strawberry plants, which comprises: a root development chamber (110a) for growing roots to a length of 8 to 9 cm; a temperature control unit (110b) for transferring the rooted plants into culture bottles to room temperature and opening the lids for 24 hours; a washing unit (110c) for cleaning the plants removed from the culture medium; a potting unit (110d) for transplanting young plants into pots filled with vermiculite growing medium; a covering device (110e) equipped with transparent polyethylene bags for tightly covering the new seedlings for three to four days and then loosely covering them for more than four days to ensure adequate air exchange; an irrigation system (110f) that uses water sprayers to provide the plantlets with moisture; a growth monitoring system (110 g) to observe the growth of the plantlets in terms of height and number of leaves; a transfer mechanism for transplanting the plantlets into soil after 15 to 20 days of growth in the culture medium; and a controlled growth chamber (110 h) with a temperature control system to maintain a temperature range of 22 °C to 23 °C and a lighting system to provide a photoperiod of 12 hours of light per day. [4] The system of claim 1, wherein the surface sterilization chamber (102) comprises a rotating platform configured to uniformly expose the strawberry runner tip explants to the ultraviolet lamps (102e) to ensure consistent sterilization across all surfaces of the explants, and wherein the surface sterilization chamber (102) comprises a rotating UV lamp assembly mounted on an automated rail system, whereby the UV lamps can be moved laterally across the chamber to ensure uniform exposure of the strawberry runner tip explants to varying UV intensities, thereby improving the thoroughness of the sterilization process, and wherein the surface sterilization chamber (102) is equipped with a two-phase airflow system configured to direct a laminar airflow across the explants,to remove surface deposits and neutralize any remaining contaminants using a targeted ionized air stream before UV sterilization begins. [5] The system of claim 1, wherein the sterile culture production chamber (104) comprises a temperature control unit with precision thermal sensors evenly distributed throughout the chamber to ensure that the temperature remains in the range of 22°C to 23°C with a deviation of no more than ±0.1°C, and wherein the sterile culture production chamber (104) comprises a dynamic humidity control system consisting of a network of microhygrometers distributed throughout the chamber, each connected to a localized ultrasonic humidifier, thereby enabling zone-specific humidity adjustments to maintain optimal humidity levels. [6] The system of claim 1, wherein the sterile culture production chamber (104) is further equipped with a programmable environmental control unit specifically designed to maintain precise temperature control in the range of 22°C to 23°C and a 12-hour photoperiod that can be adjusted according to the requirements of shoot regeneration and root formation. [7] The system according to claim 1, wherein the shoot regeneration chamber (106) comprises a humidity control system with integrated mist nozzles, designed to maintain an optimal humidity level by evenly distributing the humidity within the chamber. Furthermore, the shoot regeneration chamber (106) includes an automated explant rotation mechanism that periodically rotates the explants at precise angles, ensuring uniform light exposure and nutrient uptake from all directions, thereby promoting uniform and accelerated shoot development. Furthermore, the shoot regeneration chamber (106) includes an integrated electromagnetic pulse emitter that intermittently emits low-frequency electromagnetic pulses to stimulate cell differentiation in the explants, thereby improving the efficiency of shoot regeneration.In addition, the shoot regeneration chamber (106) has a nutrient delivery system for delivering kinetin and metatopolin and is integrated with a monitoring system that tracks shoot length and automatically adjusts nutrient supply and environmental conditions to optimize shoot growth within a period of 15 to 20 days. [8] The system of claim 1, wherein the rooting chamber (108) comprises an adjustable light panel allowing precise control of the light intensity and spectrum, specifically tuned to wavelengths that promote root development in the regenerated shoots, and wherein the rooting chamber (108) comprises a micro-oxygenation system that injects controlled microbubbles of oxygen into the culture medium surrounding the roots, thus promoting improved root growth by optimizing oxygen availability in the root zone. [9] The system according to claim 1, wherein the acclimatization chamber (110) is equipped with a ventilation system comprising adjustable air inlets and outlets designed to control air exchange and maintain the desired CO2 concentration for optimal acclimatization of the young plants, and wherein the acclimatization chamber (110) is equipped with a differential pressure air filtration system that maintains a positive pressure environment to prevent external contaminants from entering the chamber, while allowing a controlled exchange of CO2 and O2 to optimize acclimatization. [10] The system of claim 1, wherein the surface sterilization chamber (102) comprises an integrated optical particle counter that continuously monitors the air quality in the chamber and triggers an automatic UV sterilization cycle when the particle concentration exceeds a predefined threshold, thereby ensuring a contamination-free environment for the explants, and wherein the surface sterilization chamber (102) comprises a real-time contamination detection system that uses a laser-induced fluorescence sensor array that detects the presence of biological contaminants on the explants and automatically adjusts the UV sterilization parameters to ensure complete decontamination.

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