Method for culturing key kenori and method for producing feed

Abstract

To provide a method for culturing a key-shaped kenori, by which the key-shaped kenori can be cultured in a large amount in the sea, and to provide a method for producing a feed formulated with the key-shaped kenori.SOLUTION: The method for culturing a hooked kenori according to the present invention is a method for culturing a hooked kenori using a culture plate, wherein the culture plate has a plate body and a plurality of second protrusions protruding from one surface side of the plate body, and the method for culturing a hooked kenori comprises the following steps (1) to (3): Step (1): Step of culturing sporophores in which tetrasporangia are formed using a culture plate Step (2): Step of culturing young gametophytes using the culture plate in which the sporophores in which tetrasporangia are formed are cultured in Step (1) Step (3): Step of culturing gametophytes in the sea using the culture plate in which the young gametophytes are cultured in Step (2) and collecting the gametophytes SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a method for cultivating Porphyra japonica and a method for producing a feed. [Background technology]

[0002] Traditionally, methane emissions from the burps of livestock such as cows have been considered a cause of global warming. In Japan, the Ministry of Agriculture, Forestry and Fisheries' Green Food System Strategy includes the "utilization of methane-reducing cows" as one of the initiatives to be implemented by 2050. As part of this initiative, attention is being paid to the development of feed that reduces methane emissions from cow burps.

[0003] The addition of the red algae Asparagops taxiformis (scientific name: Asparagops taxiformis) to livestock feed has attracted attention as a substance that can suppress the emission of methane from livestock burps. Because Asparagops taxiformis contains a large amount of bromoform, it is thought that feeding livestock feed containing Asparagops can suppress the synthesis of methane in the livestock's intestines through bromoform.

[0004] However, since the algae are very fragile and crumble when harvested from the sea, it is difficult to efficiently recover algae grown on rocky reefs from the sea. Therefore, in recent years, methods have been used in which algae harvested from natural sea areas are cultured on land using aquariums (for example, the method described in Patent Document 1). [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Patent No. 7449455 Summary of the Invention [Problem to be solved by the invention]

[0006] However, with the above-mentioned land-based cultivation methods, it is difficult to mass-cultivate Uncaria japonica because it is not easy to set conditions suitable for cultivation similar to those in the natural environment, such as light, water temperature changes, waves, and nutrients. Therefore, there is a need for a method for mass-cultivating Uncaria japonica by culturing it in the sea, which is the natural environment itself.

[0007] Therefore, the present invention has been made in consideration of the above circumstances, and its objective is to provide a method for cultivating algae that can be mass-produced in the sea, and a method for producing feed containing algae. [Means for solving the problem]

[0008] The method for cultivating Porphyra japonica according to the present invention comprises the steps of: A method for culturing Porphyra japonica using a culture plate, comprising: the culture plate has a plate body and a plurality of second protrusions protruding from one surface side of the plate body; A method for cultivating Porphyra japonica, comprising the following steps (1) to (3): Step (1): Culturing sporophytes on which tetrasporangia have been formed using a culture plate Step (2): Culturing young gametophytes using the culture plate on which the sporophytes on which tetrasporangia have been formed in step (1) have been cultured. Step (3): Culturing and recovering gametophytes in the sea using the culture plate on which the juvenile gametophytes were cultured in step (2).

[0009] According to this configuration, the method for cultivating Uncaria japonica can culture the juvenile gametophytes in the sea until they become gametophytes, while preventing the juvenile gametophytes from being released into the sea due to the influence of ocean currents, waves, etc. Therefore, the method for cultivating Uncaria japonica can culture a large amount of Uncaria japonica in the sea.

[0010] In one aspect, the method for cultivating Porphyra japonica according to the present invention comprises: Step (1) is Step (1-1): placing a first culture plate in a first water tank; Step (1-2): A step of introducing a gametophyte having a carposporophyte into a first tank, thereby releasing carpospores from the carposporophyte into the first tank; and Step (1-3): A step of implanting the carpospores between adjacent second protrusions of the first culture plate, thereby forming sporophytes from the implanted carpospores, and growing the sporophytes until tetrasporangia are formed, thereby culturing the sporophytes with tetrasporangia formed; Including, Step (2) is Step (2-1): Recovering a first culture plate having sporangia formed thereon from a first water tank and placing the first culture plate in a second water tank; and Step (2-2): A step of implanting the tetraspores released from the tetrasporangia between adjacent second protrusions of a second culture plate placed in a second water tank, thereby forming juvenile gametophytes from the tetraspores and allowing the stolon of the juvenile gametophyte and the rhizoid threads extending from the stolon to attach to the second protrusions, thereby culturing the juvenile gametophyte; Including, Step (3) is Step (3-1): recovering the second culture plate to which the juvenile gametophytes are attached from the second tank and submerging it in the sea, thereby culturing the gametophytes; and Step (3-2): recovering the cultured gametophytes together with the second culture plate from the sea; Includes:

[0011] According to this configuration, the method for cultivating Porphyra can efficiently cultivate young gametophytes by fixing the tetraspores released from the sporophyte, which has formed tetrasporangia fixed to the first culture plate, to the second culture plate in the second tank, thereby efficiently implanting the tetraspores between adjacent second protrusions on the second culture plate.

[0012] In the method for cultivating Uncaria nigra according to the present invention, steps (1) to (3) may be carried out at a water temperature of 20°C or higher and 35°C or lower.

[0013] According to the above-described configuration, the method for cultivating Porphyra can cultivate Porphyra in an environment similar to the habitat of Porphyra, thereby further promoting the cultivation of Porphyra.

[0014] In the method for culturing Uncaria nigra according to the present invention, the gametophyte having the carposporophyte in step (1-2) may be derived from a recovered gametophyte.

[0015] According to the above-described method for cultivating Uncaria japonica, Uncaria japonica can be efficiently cultivated.

[0016] In the method for cultivating Uncaria nigra according to the present invention, the distance between adjacent second protrusions in the plurality of second protrusions may be 0.1 mm or more and 5.0 mm or less.

[0017] According to the above-described configuration, the method for cultivating Uncaria japonica can easily fix the rhizoids extending from the stolon of Uncaria japonica to the second protruding portion, thereby facilitating the cultivation of Uncaria japonica.

[0018] In the method for cultivating Uncaria nigra according to the present invention, the second protruding portion may be cylindrical in shape and have a diameter of 1.0 mm or more and 5.0 mm or less.

[0019] According to the above-described configuration, the method for cultivating Uncaria japonica can easily fix the rhizoids extending from the stolon of Uncaria japonica to the second protruding portion, thereby facilitating the cultivation of Uncaria japonica.

[0020] The method for producing feed according to the present invention is a method for producing livestock feed, and comprises the following steps (1') to (3'). Step (1'): A step of culturing Uncaria japonica using the above-mentioned method for culturing Uncaria japonica Step (2'): A step of drying the algae cultured in step (1'). Step (3'): A step of incorporating the dried algae obtained in step (2') as a raw material for feed.

[0021] The feed production method includes step (1'), which allows efficient production of a large amount of Uncaria arborescens, and therefore allows efficient mass production of feed containing Uncaria arborescens. [Effects of the Invention]

[0022] According to the present invention, it is possible to provide a method for cultivating Uncaria nigra in large quantities in the sea, and a method for producing feed containing Uncaria nigra. [Brief explanation of the drawings]

[0023] [Figure 1] FIG. 1 is a perspective view of a culture plate 2 used in the method for cultivating Uncaria nigra according to this embodiment. [Figure 2] FIG. 2 is a diagram showing how the second culture plate 2 to which the juvenile gametophytes are fixed is submerged in the sea in one embodiment of the step (3-1). [Figure 3] FIG. 3 is a diagram showing another embodiment of step (3-1) in which the second culture plate 2 to which the juvenile gametophytes are fixed is submerged in the sea. [Figure 4] FIG. 4 is a diagram illustrating a method for culturing Uncaria nigra using a culture plate 2 on which seed threads B of Uncaria nigra are fixed. DETAILED DESCRIPTION OF THE INVENTION

[0024] Hereinafter, embodiments of the present invention will be described, but the present invention is not limited to the following embodiments.

[0025] (How to cultivate Kagikenori) Hereinafter, a method for cultivating Porphyra japonica according to an embodiment of the present invention will be described with reference to the drawings. In the following drawings, the same or corresponding parts are designated by the same reference numerals, and the description thereof will not be repeated.

[0026] The method for cultivating Porphyra japonica according to this embodiment includes the steps of: A method for culturing Porphyra japonica using a culture plate 2, The culture plate 2 has a plate body 21 and a plurality of second protrusions 23 protruding from one surface S of the plate body 21, The method comprises the following steps (1) to (3). Step (1): Culturing sporophytes on which tetrasporangia have been formed using a culture plate 2 Step (2): A step of culturing young gametophytes using the culture plate 2 on which the sporophytes on which tetrasporangia have been formed in step (1) have been cultured. Step (3): A step of culturing and recovering gametophytes in the sea using the culture plate 2 on which the juvenile gametophytes were cultured in step (2).

[0027] Steps (1) to (3) are preferably carried out at a water temperature of 20°C or higher and 35°C or lower, from the viewpoint of further promoting the cultivation of the lockweed by culturing the lockweed in an environment similar to its habitat.

[0028] [Culture plate] 1 is a perspective view of a culture plate 2 used in the method for cultivating Uncaria nigra according to this embodiment. The culture plate 2 has a plate body 21, a plurality of first protrusions 22, and a plurality of second protrusions 23.

[0029] The plurality of first protrusions 22 protrude from one surface S side of the plate body 21 and are configured to be able to fix seed threads B of the algae seaweed shown in FIG. 4, which will be described later.

[0030] The shape of the first protrusion 22 is cylindrical so as to make it easier to wind the seed thread B of the Porphyra japonica around the first protrusion. The diameter of the first protrusion 22 is preferably 5 mm or more and 15 mm or less, and more preferably 7 mm or more and 13 mm or less. However, the shape, diameter, etc. of the first protrusion 22 are not limited thereto as long as the seed thread B of the Porphyra japonica can be fixed thereto.

[0031] The height of the first protrusion 22 is preferably 10 mm or more and 20 mm or less, more preferably 12 mm or more and 18 mm or less, from the viewpoint of preventing the tip of the gametophyte A from colliding with the first protrusion 22 and collapsing while making it easier to wind the seed thread B of the algae around the first protrusion 22.

[0032] When the plate body 21 is viewed from one surface S, ten first protrusions 22 are formed on each side of a pair of symmetrical portions (hereinafter referred to as "hook thread placement portions S1, S1") that are sandwiched between a line L connecting the centers of opposing long sides of the four sides that make up the periphery of the plate body 21, i.e., a total of 20 first protrusions 22 are formed throughout the plate body 21. Note that the number of first protrusions 22 is not limited to this as long as the hook threads B can be fixed thereto.

[0033] In each of the pair of algae arrangement sections S1, S1, the ten first protrusions 22 are arranged in three rows parallel to the long sides of the plate body 21, of which the outer two rows are made up of four first protrusions 22 and the central row is made up of two first protrusions 22. The arrangement, spacing, etc. of the first protrusions 22 are not particularly limited.

[0034] The second protrusions 23 protrude from one surface S of the plate body 21 and are configured to be able to fasten rhizoid threads A1 extending from the stolon of the gametophyte A shown in FIG. 4, which will be described later.

[0035] The distance between adjacent second protrusions 23, 23 is preferably 0.1 mm or more and 5.0 mm or less, and more preferably 0.5 mm or more and 2.0 mm or less, from the viewpoint of making it easier for the adventitious root thread A1 extending from the stolon of the gametophyte A to attach to the second protrusion 23.

[0036] The shape of the second protrusion 23 is cylindrical from the viewpoint of facilitating the anchoring of the rhizoid threads A1 extending from the stolon of the gametophyte A to the second protrusion 23. The diameter of the second protrusion 23 is preferably 1.0 mm or more and 5.0 mm or less, and more preferably 1.2 mm or more and 3.0 mm or less. The shape, diameter, etc. of the second protrusion 23 are not limited as long as the rhizoid threads A1 extending from the stolon of the gametophyte A can be anchored thereto. In one aspect of the method for cultivating Uncaria japonica according to this embodiment, the shape of the second protrusion 23 is cylindrical and the diameter is 1.0 mm or more and 5.0 mm or less.

[0037] The height of the second protrusion 23 is not particularly limited as long as it is capable of anchoring the rhizoid thread A1 extending from the stolon of the gametophyte A, and may be, for example, 1.0 mm or more and 5.0 mm or less, or 2.0 mm or more and 4.0 mm or less.

[0038] The same number of second protrusions 23 are formed on each of the pair of arrangement portions S1, S1 of the algae. The number of second protrusions 23 is greater than the number of first protrusions 22. The number of second protrusions 23 is not particularly limited as long as it is possible for carospores, sporophytes with tetrasporangia formed, tetraspores, young gametophytes, and gametophytes to adhere.

[0039] The second protrusions 23 are arranged symmetrically across the line L in the arrangement portions S1, S1 of the pair of hooks. The second protrusions 23 are also arranged around the first protrusions 22.

[0040] As shown in FIG. 1 , the culture plate 2 has a plurality of convex portions 24 formed between the plurality of first protrusions 22 and the plurality of second protrusions 23 on one surface S of the plate body 21 (i.e., the surface from which the first protrusions 22 and the second protrusions 23 protrude). The shape, size, number, etc. of the convex portions constituting the convex portions 24 are not particularly limited, but the height of the convex portions 24 is preferably configured to be lower than the height of the second protrusions 23, for example, 5.0 mm or less. By forming the plurality of convex portions 24 on the culture plate 2, it is possible to prevent shellfish and the like that may inhibit the growth of the algae from settling on one surface of the plate body 21, and it is also possible to fixate the adventitious root threads A1 extending from the stolon of the gametophyte A to the convex portions constituting the plurality of convex portions 24.

[0041] 1, the culture plate 2 has a hole 25 formed in the center of one surface S of the plate body 21, i.e., at a position corresponding to the space between the pair of algae placement sections S1, S1. The hole 25 is configured so that when the culture plate 2 is fixed to the concrete block 3 as described below, a bolt 27 can be inserted into the hole 25 to fix the culture plate 2 to the concrete block 3.

[0042] As shown in Fig. 1, the culture plate 2 has a pair of notches 26, 26 formed inward from the edge on opposing short sides of the four sides that form the periphery of the culture plate 2. The pair of notches 26, 26 are formed parallel to one another with a gap in the center of one short side. The positions at which the pair of notches 26, 26 are formed are not limited to the opposing pair of short sides, and the pair of notches 26, 26 may be formed on any of the four sides that form the periphery of the culture plate 2. The culture plate 2 has the pair of notches 26, 26, which allows the end of the seed thread B of the Porphyra japonica to be hooked and fixed into the pair of notches 26, 26.

[0043] The material of the culture plate 2 is not particularly limited as long as it can support carpospores, sporophytes with tetrasporangia formed, and tetraspores, and examples thereof include synthetic resins and biodegradable resins.

[0044] Examples of synthetic resins include polyethylene, polypropylene, polyvinyl chloride, polystyrene, syndiotactic polystyrene, ABS resin, AES resin, polyamide, polyacetal, polyester, polycarbonate, modified polyphenylene ether, polysulfone, polyarylate, polyetherimide, polyamideimide, polyphenylene sulfide, liquid crystal polyester, PEEK, PEN, paraffin wax, montan wax, carnauba wax, fatty acid ester, glycerite, modified wax, and silane-modified polyolefin polymer.

[0045] Examples of biodegradable resins include polyvinyl alcohol, polyglycolic acid, polybutylene succinate, polybutylene succinate-co-adipate, polybutylene adipate terephthalate, polyethylene terephthalate succinate, polylactic acid, polyhydroxyalkanoate, 3-hydroxybutyric acid-3-hydroxyhexane copolymer polyester, biopolybutylene succinate, polybutylene adipate terephthalate-polylactic acid compound, starch polyester resin, and cellulose acetate.

[0046] The synthetic resin and biodegradable resin may contain an inorganic filler, such as silica powder, calcium carbonate powder, limestone powder, whitebait powder, bone stone powder, diatomaceous earth powder, clay powder, shell powders such as oyster, scallop, turban shell, ark shell, whelk, clam, and shijimi, and coral powder. The inclusion of the inorganic filler improves the strength of the culture plate 2 and facilitates the growth of the algae.

[0047] The shape of the culture plate 2 in a plan view is rectangular, as shown in Fig. 1. The shape of the culture plate in a plan view is not particularly limited, and may be, for example, a trapezoid, a parallelogram, a diamond, or the like, other than a rectangular shape.

[0048] [Process (1)] In step (1), the sporophyte on which tetrasporangia have been formed is cultured using a culture plate 2.

[0049] Specifically, in one embodiment, step (1) is Step (1-1): placing a first culture plate 2 in a first water tank; Step (1-2): A step of introducing a gametophyte having a carposporophyte into a first tank, thereby releasing carpospores from the carposporophyte into the first tank; and Step (1-3): A step of implanting carpospores between adjacent second protrusions 23, 23 of the first culture plate 2, thereby forming sporophytes from the implanted carpospores, and growing the sporophytes until tetrasporangia are formed, thereby culturing the sporophytes with tetrasporangia formed; Includes:

[0050] <Process (1-1)> In step (1-1), a first culture plate 2 is placed in a first water tank.

[0051] The first culture plate 2 is similar to the culture plate 2 described above.

[0052] The first culture plate 2 may be placed, for example, by suspending it from above the first tank, by placing it on the bottom of the first tank, or by placing it on the side of the first tank. Of these, the preferred method of placing the first culture plate 2 is by placing it on the bottom of the first tank, from the viewpoint of facilitating the implantation of carpospores in step (1-3) described below.

[0053] The number of first culture plates 2 placed in the first water tank is not particularly limited, and may be one, or two or more.

[0054] Furthermore, when two or more first culture plates 2 are installed, it is preferable that the first culture plates 2 are arranged without any gaps, from the viewpoint of efficiently attaching carpospores to the first culture plates 2.

[0055] <Process (1-2)> In step (1-2), a gametophyte having a carposporophyte is placed in a first tank, whereby carpospores are released from the carposporophyte into the first tank.

[0056] From the viewpoint of efficiently culturing Uncaria japonica, the gametophyte having the carposporophyte is preferably derived from a recovered gametophyte.

[0057] Examples of recovered gametophytes include gametophytes recovered from marine habitats of Undaria pinnatifida, gametophytes grown in an aquarium and recovered from the aquarium, etc. Among these, the recovered gametophytes are preferably gametophytes recovered from marine habitats of Undaria pinnatifida, from the viewpoint that gametophytes that have grown sufficiently in a natural environment can be used to release more carpospores.

[0058] Examples of ways to introduce gametophytes having carposporophytes include a method of introducing water containing gametophytes having carposporophytes into a first tank, and a method of placing a culture plate 2 to which gametophytes having carposporophytes are attached in the first tank.

[0059] In addition, the culture plate 2 to which the gametophyte having the above-mentioned carposporophyte is fixed may be formed, for example, by a method in which a gametophyte having a carposporophyte floating in the sea is allowed to naturally implant on one surface of the culture plate 2; or by a method in which a gametophyte having a collected carposporophyte is artificially implanted on one surface S of the culture plate 2.

[0060] <Process (1-3)> In step (1-3), the carpospores are implanted between adjacent second protrusions 23, 23 of the first culture plate 2, and sporophytes are formed from the implanted carpospores. The sporophytes are then grown until tetrasporangia are formed, and the sporophytes with tetrasporangia formed are cultured.

[0061] [Process (2)] In step (2), the juvenile gametophytes are cultured using the culture plate 2 on which the sporophytes on which tetrasporangia have been formed in step (1) have been cultured.

[0062] Specifically, in one embodiment, step (2) is Step (2-1): A step of recovering the first culture plate 2 to which the sporophytes with tetrasporangia formed thereon are attached from the first water tank, and placing the first culture plate 2 in a second water tank; and Step (2-2): A step of implanting the tetraspores released from the tetrasporangia between the adjacent second protrusions 23, 23 of the second culture plate 2 placed in the second water tank, thereby forming juvenile gametophytes from the tetraspores and culturing the juvenile gametophytes by fixing the stolon of the juvenile gametophyte and the rhizoids extending from the stolon to the second protrusions 23; Includes:

[0063] <Process (2-1)> In step (2-1), the first culture plate 2 to which the sporophytes on which tetrasporangia have been formed are attached is collected from the first water tank, and the first culture plate 2 is placed in a second water tank.

[0064] The first culture plate 2 having sporophytes with tetrasporangia formed thereon can be placed, for example, by suspending the first culture plate 2 from above the second tank, by placing the first culture plate 2 on the bottom of the second tank, or by placing the first culture plate 2 on the side of the second tank. Among these, the first culture plate 2 is preferably placed by suspending the first culture plate 2 from above the second tank or by placing the first culture plate 2 on the side of the second tank, from the viewpoint of facilitating efficient diffusion of the tetraspores described below into the second tank, and more preferably by suspending the first culture plate 2 from above the second tank.

[0065] The number of first culture plates 2 with sporophytes with tetrasporangia formed attached thereto that are placed in the second tank is not particularly limited, and may be one or two or more.

[0066] <Process (2-2)> In step (2-2), the tetrads released from the tetrasporangia are implanted between adjacent second protrusions 23, 23 of the second culture plate 2 placed in the second tank, whereby a juvenile gametophyte is formed from the tetrads, and the stolon of the juvenile gametophyte and the rhizoid threads extending from the stolon are fixed to the second protrusions 23, thereby culturing the juvenile gametophyte.

[0067] The second culture plate 2 is similar to the culture plate 2 described above.

[0068] Examples of the manner in which the second culture plate 2 is placed include a manner in which the second culture plate 2 is suspended from above the second tank, a manner in which the second culture plate 2 is placed on the bottom surface of the second tank, a manner in which the second culture plate 2 is placed on the side surface of the second tank, etc. Among these, the manner in which the second culture plate 2 is placed is preferably a manner in which the second culture plate 2 is placed on the bottom surface of the second tank, from the viewpoint of facilitating the implantation of the tetraspores.

[0069] The number of second culture plates 2 placed in the second water tank is not particularly limited, and may be one, or two or more.

[0070] Furthermore, when two or more second culture plates 2 are installed, the second culture plates 2 are preferably arranged without any gaps, from the viewpoint of efficiently attaching the tetraspores to the second culture plates 2.

[0071] [Process (3)] In step (3), the gametophytes are cultured in the sea using the culture plate 2 on which the juvenile gametophytes were cultured in step (2), and then recovered.

[0072] Specifically, in one embodiment, step (3) is Step (3-1): A step of recovering the second culture plate 2 to which the juvenile gametophytes are attached from the second tank and submerging it in the sea to culture the gametophytes; and Step (3-2): recovering the cultured gametophytes together with the second culture plate 2 from the sea; Includes:

[0073] <Process (3-1)> In step (3-1), the second culture plate 2 on which the juvenile gametophytes are fixed is collected from the second tank and submerged in the sea, whereby gametophytes A are cultured.

[0074] In one embodiment of step (3-1), as shown in Figure 2, a second culture plate 2 to which juvenile gametophytes are adhered is submerged in the sea to culture gametophytes A. Figure 2 is a diagram showing how the second culture plate 2 to which juvenile gametophytes are adhered is submerged in the sea in one embodiment of step (3-1). As shown in Figure 2, the second culture plate 2 to which juvenile gametophytes are adhered (hereinafter simply referred to as culture plate 2) is attached to at least a portion of the surface T of a concrete block 3.

[0075] 2, the culture plate 2 is placed across the concrete block 3 at the center of the surface T of the concrete block 3. The position at which the culture plate 2 is placed can be changed as appropriate depending on the size, shape, etc. of the culture plate 2.

[0076] The culture plate 2 is fixed by inserting bolts 27 into holes 25 (shown in FIG. 1) and fitting the bolts 27 into holes (not shown) provided in the surface of the concrete block 3 .

[0077] The concrete block 3 has a plurality of protrusions 31 formed on the surface T where the culture plate 2 is not attached.

[0078] The shape of the protrusions 31 is rock-like, as shown in Fig. 2. However, the shape of the protrusions 31 is not particularly limited.

[0079] The height of the protrusion 31 is not particularly limited, and may be, for example, 5.0 mm or more and 30.0 mm or less.

[0080] The distance between adjacent protrusions 31, 31 is not particularly limited, and may be, for example, 5.0 mm or more and 40.0 mm or less.

[0081] The concrete block 3 has a plurality of protrusions 31 formed on the surface T where the culture plate 2 is not attached, allowing the algae to naturally settle on the plurality of protrusions 31. The arrangement of the plurality of protrusions 31 is not particularly limited and may be, for example, irregular.

[0082] In another embodiment of step (3-1), as shown in Figure 3, the second culture plate 2 to which the juvenile gametophytes are fixed is submerged in the sea to culture the gametophytes A. Figure 3 is a diagram showing how the second culture plate 2 to which the juvenile gametophytes are fixed is submerged in the sea in one embodiment of step (3-1). As shown in Figure 3, the culture plate 2 further includes a seaweed bed growth reef 4 in addition to the above-mentioned concrete block 3, and a plurality of pedestals 5 each equipped with the culture plate 2 are arranged on the upper surface of the seaweed bed growth reef 4.

[0083] 3, the upper surface of the base 5 is provided with a culture plate 2 attached to a concrete block 3. Although the culture plate 2 is attached to the concrete block 3 in FIG. 3, the culture plate 2 does not have to be attached to the concrete block 3.

[0084] The height of the base 5 is not particularly limited, and may be, for example, 0.3 m or more and 0.4 m or less.

[0085] The area of ​​the top surface of the base 5 is not particularly limited as long as it can accommodate at least one concrete block 3, and may be, for example, 0.2 m 2 More than 0.3m 2 It may be the following:

[0086] The shape of the base 5 is not particularly limited, and may be, for example, a square, a rectangle, or the like when viewed from above.

[0087] The number of pedestals 5 arranged on the upper surface of the seaweed bed reef 4 can be changed as appropriate depending on the area of ​​the upper surface of the seaweed bed reef 4 on which a plurality of pedestals 5 (described later) are arranged.

[0088] The height of the seaweed bed reef 4 is not particularly limited as long as it can be installed in the sea, and may be, for example, 0.8 m or more and 1.0 m or less.

[0089] The area of ​​the upper surface of the seaweed bed reef 4 is not particularly limited as long as it is possible to arrange a plurality of pedestals 5 equipped with culture plates 2. For example, it may be 6.0 m 2 Over 8.0m 2 It may be the following:

[0090] The shape of the seaweed bed reef 4 is a substantially hexagonal prism, as shown in Fig. 3. The shape of the seaweed bed reef 4 is not particularly limited, and can be changed as appropriate depending on the environment, such as the location where it is installed and the ocean currents.

[0091] As shown in Fig. 3, the seaweed bed reef 4 is provided with a cubic fence 6 that covers the pedestal 5 on which the plurality of culture plates 2 are mounted. By providing the fence 6, the seaweed bed reef 4 can protect the algae from being eaten by fish and the like. The shape of the fence 6 is not particularly limited as long as it can cover the pedestal 5 on which the plurality of culture plates 2 are mounted. The seaweed bed reef 4 does not necessarily have to be provided with a fence 6.

[0092] As shown in Figure 3, the upper surface of the seaweed bed reef 4 is provided with a hooking portion 7 that can be suspended using a crane or the like. The hooking portion 7 allows the seaweed bed reef 4 to be pulled up from the sea together with the culture plate 2, making it possible to collect a large amount of algae without breaking them down. In Figure 3, the hooking portion 7 is provided on the upper surface where multiple pedestals 5 equipped with culture plates 2 are arranged, but the location of the hooking portion 7 is not particularly limited as long as it is possible to collect the algae without breaking them down. The shape of the hooking portion 7 is an inverted U-shape relative to the upper surface of the seaweed bed reef 4, but this shape is not particularly limited.

[0093] In addition, as another embodiment of step (3-1), although not shown, the gametophytes may be cultured by attaching one or more culture plates 2 to a floating member such as a raft with a rope or the like, and hanging it down and sinking it in the sea.

[0094] From the viewpoint of culturing gametophytes under appropriate culture conditions, the location where the culture plate 2 is submerged is preferably a location with a water depth of 0.1 m or more and 15.0 m or less, more preferably a location with a water depth of 0.3 m or more and 10.0 m or less.

[0095] In step (3-2), the cultured gametophyte A is collected from the sea together with the second culture plate 2.

[0096] Examples of methods for recovering the cultured gametophytes A together with the second culture plate 2 from the sea include a method of recovering them by diving, a method of pulling them up using a crane, etc. In the step (3-1), when the method of sinking the culture plate 2 is a method of attaching one or more culture plates 2 to a floating member such as a raft with a string or the like and lowering it into the sea, the method of recovering the cultured gametophytes A together with the culture plate 2 from the sea can be, in addition to the above recovery method, a method of recovering the cultured gametophytes A together with the culture plate 2 from the sea by reeling in the rope to which the culture plate 2 is attached.

[0097] In a preferred embodiment, the method for cultivating Porphyra japonica according to the present embodiment includes the following steps: Step (1) is Step (1-1): placing a first culture plate 2 in a first water tank; Step (1-2): A step of introducing a gametophyte having a carposporophyte into a first tank, thereby releasing carpospores from the carposporophyte into the first tank; and Step (1-3): A step of implanting carpospores between adjacent second protrusions 23, 23 of the first culture plate 2, thereby forming sporophytes from the implanted carpospores, and growing the sporophytes until tetrasporangia are formed, thereby culturing the sporophytes with tetrasporangia formed; Including, Step (2) is Step (2-1): A step of recovering the first culture plate 2 to which the sporophytes with tetrasporangia formed thereon are attached from the first water tank, and placing the first culture plate 2 in a second water tank; and Step (2-2): A step of implanting the tetraspores released from the tetrasporangia between adjacent second protrusions 23, 23 of a second culture plate 2 placed in a second water tank, thereby forming juvenile gametophytes from the tetraspores and fixing the stolon of the juvenile gametophyte and the rhizoids extending from the stolon to the second protrusion 2, thereby culturing the juvenile gametophyte; Including, Step (3) is Step (3-1): A step of recovering the second culture plate 2 to which the juvenile gametophytes are attached from the second tank and submerging it in the sea, thereby culturing the gametophytes A; and Step (3-2): recovering the cultured gametophyte A together with the second culture plate 2 from the sea; Includes:

[0098] The method for cultivating Kagikenori according to this embodiment may be carried out by previously fixing Kagikenori seed threads B to the first protruding portion 22 of the first culture plate 2 and / or the second culture plate 2, as shown in Figure 4. Figure 4 is a diagram illustrating a method for cultivating Kagikenori using the culture plate 2 to which Kagikenori seed threads B are fixed.

[0099] As used herein, seed filament refers to a filament having attached thereto a gametophyte having a carposporophyte, a carpospore, a sporophyte having formed tetrasporangia, a tetraspore, a young gametophyte, or a gametophyte A.

[0100] The seed thread B of the algae is not particularly limited as long as it is flexible and strong enough to allow attachment of carpospores, sporophytes with tetrasporangia formed, and tetraspores, and can be fixed to the culture plate 2, and examples include twisted threads such as rope and string, wire, tape, etc.

[0101] The outer diameter of the seed threads B of the Porphyra japonica is not particularly limited, and may be, for example, 1.0 mm or more and 40.0 mm or less.

[0102] 4, the method for fixing the Nori seaweed seed thread B is as follows: first, one end of the Nori seaweed seed thread B is sandwiched into one of the pair of notches 26, 26 of the culture plate 2 from one surface S side to the other surface S' side of the culture plate 2, and the Nori seaweed seed thread B is attached to the culture plate 2 so that one end of the Nori seaweed seed thread B is positioned on the other surface S' side of the culture plate 2. Then, the Nori seaweed seed thread B with one end fixed is hooked along the circumferential surface of each first protrusion 22 on the one surface S side of the culture plate 2, and engaged while changing direction, and then stretched between each first protrusion 22, thereby fixing the Nori seaweed seed thread B to the first protrusion 22 of the culture plate 2.

[0103] 4, the method for fixing the Nori seaweed seed thread B is to wrap the Nori seaweed seed thread B around the first protrusion 22, and then to sandwich the other end of the Nori seaweed seed thread B into one of the pair of notches 26, 26 in the culture plate 2 that does not have one end of the Nori seaweed seed thread B sandwiched therein, from one surface S side to the other surface S' side of the culture plate 2, similar to the one end of the Nori seaweed seed thread B, and to fix the other end of the Nori seaweed seed thread B to the culture plate 2. In other words, by fixing both ends of the Nori seaweed seed thread B to the pair of notches 26, 26 in the culture plate 2, the Nori seaweed seed thread B can be attached more reliably to the culture plate 2.

[0104] The distance between the seed threads B of the algae and one surface S of the culture plate 2 is not particularly limited as long as it is possible to fix the rhizoid threads extending from the stolon of the gametophyte A to the second protrusion 23 while keeping the seed threads B of the algae under a moderate tension, and may be, for example, 30 mm or more and 50 mm or less.

[0105] Furthermore, by performing the above step (1-3) using the first culture plate 2 on which the seed threads B of the algae are fixed, the carpospores can be implanted not only between adjacent second protrusions 23, 23 of the first culture plate 2, but also on the seed threads B of the algae.

[0106] Furthermore, by performing the above step (2-2) using a second culture plate 2 on which the seed threads B of the algae are fixed, the tetraspores can be implanted not only between adjacent second protrusions 23, 23 of the first culture plate 2, but also on the seed threads B of the algae.

[0107] The method for cultivating Porphyra japonica according to this embodiment includes the steps of: A method for culturing Porphyra japonica using a culture plate 2, The culture plate 2 has a plate body 21 and a plurality of second protrusions 23 protruding from one surface S of the plate body 21, A method for cultivating Porphyra japonica, comprising the following steps (1) to (3): Step (1): Culturing sporophytes on which tetrasporangia have been formed using a culture plate 2 Step (2): A step of culturing young gametophytes using the culture plate 2 on which the sporophytes on which tetrasporangia have been formed in step (1) have been cultured. Step (3): A step of culturing and recovering gametophytes A in the sea using the culture plate 2 on which the juvenile gametophytes were cultured in step (2).

[0108] In the method for cultivating Uncaria japonica, the juvenile gametophytes are fixed to the culture plate 2 by such a configuration, and therefore the juvenile gametophytes can be cultured in the sea until they become gametophytes A while being prevented from being released into the sea due to the influence of ocean currents, waves, etc. Therefore, the method for cultivating Uncaria japonica can cultivate a large amount of Uncaria japonica in the sea.

[0109] As one aspect of the method for cultivating Porphyra japonica according to this embodiment, Step (1) is Step (1-1): placing a first culture plate 2 in a first water tank; Step (1-2): A step of introducing a gametophyte having a carposporophyte into a first tank, thereby releasing carpospores from the carposporophyte into the first tank; and Step (1-3): A step of implanting carpospores between adjacent second protrusions 23, 23 of a first culture plate, thereby forming sporophytes from the implanted carpospores, and growing the sporophytes until tetrasporangia are formed, thereby culturing the sporophytes with tetrasporangia formed; Including, Step (2) is Step (2-1): A step of recovering the first culture plate 2 to which the sporophytes with tetrasporangia formed thereon are attached from the first water tank, and placing the first culture plate 2 in a second water tank; and Step (2-2): A step of implanting the tetraspores released from the tetrasporangia between the adjacent second protrusions 23, 23 of the second culture plate 2 placed in the second water tank, thereby forming juvenile gametophytes from the tetraspores and culturing the juvenile gametophytes by fixing the stolon of the juvenile gametophyte and the rhizoids extending from the stolon to the second protrusions 23; Including, Step (3) is Step (3-1): A step of recovering the second culture plate 2 to which the juvenile gametophytes are attached from the second tank and submerging it in the sea, thereby culturing the gametophytes A; and Step (3-2): recovering the cultured gametophyte A together with the second culture plate 2 from the sea; Includes:

[0110] With this configuration, the method for cultivating algae described above allows the tetraspores released from the sporophyte, which has formed tetrasporangia fixed to the first culture plate 2, to be fixed to the second culture plate 2 in the second tank, so that the tetraspores can be efficiently implanted between adjacent second protrusions 23, 23 on the second culture plate 2, thereby efficiently culturing young gametophytes.

[0111] In the method for cultivating algae of this embodiment, steps (1) to (3) are carried out at a water temperature of 20°C or higher and 35°C or lower, thereby allowing algae to be cultivated in an environment similar to the habitat of algae, thereby further promoting the cultivation of algae.

[0112] In the method for cultivating Uncaria nigra according to this embodiment, the gametophyte having the carposporophyte in step (1-2) is derived from the recovered gametophyte, and therefore Uncaria nigra can be cultivated efficiently.

[0113] In the method for cultivating algae of this embodiment, the spacing between adjacent second protrusions 23, 23 in the multiple second protrusions 23 is 0.1 mm or more and 5.0 mm or less, which makes it easy to fix the adventitious root threads extending from the stolon of algae to the second protrusions 23, thereby making it easy to cultivate algae.

[0114] In the method for cultivating algae of this embodiment, the second protrusion is cylindrical in shape and has a diameter of 1.0 mm or more and 5.0 mm or less, which makes it easy to fix the adventitious root threads extending from the stolon of algae to the second protrusion 23, making it easy to cultivate algae.

[0115] The method for cultivating Uncaria japonica according to the present invention is not limited to the above embodiment and can be modified in various ways.

[0116] The method for cultivating Uncaria japonica according to the above embodiment has been described using a first culture plate 2 and a second culture plate 2. However, the method for cultivating Uncaria japonica according to the present invention is not limited to this, and may be performed using, for example, only one culture plate 2.

[0117] In addition, in the method for cultivating Uncaria japonica according to the above embodiment, the first and second aquariums are used. However, the method for cultivating Uncaria japonica according to the present invention is not limited to this and may be carried out using, for example, only one aquarium.

[0118] Furthermore, in the method for cultivating Uncaria japonica according to the above embodiment, the culture plate 2 has been described as having the first protrusion 22. However, the method for cultivating Uncaria japonica according to the present invention is not limited to this, and for example, the culture plate 2 does not have to have the first protrusion 22.

[0119] (Feed manufacturing method) The method for producing feed according to this embodiment is a method for producing livestock feed, and includes the following steps (1') to (3'). Step (1'): Cultivating Kagikenori using a method for cultivating Kagikenori Step (2'): A step of drying the algae cultured in step (1'). Step (3'): A step of incorporating the dried algae obtained in step (2') as a raw material for feed.

[0120] [Process (1')] In step (1'), Uncaria nigra is cultured using a method for culturing Uncaria nigra.

[0121] The method for cultivating Uncaria nigra is the same as the method for cultivating Uncaria nigra according to the present embodiment described above.

[0122] [Process (2')] Step (2'): The Porphyra japonica cultivated in step (1') is dried.

[0123] The method for drying the algae is not particularly limited, and examples thereof include natural drying and drying at high temperatures.

[0124] The drying temperature for the algae is not particularly limited, and may be, for example, 10°C or higher and 90°C or lower, or 20°C or higher and 80°C or lower.

[0125] [Process (3')] In step (3'), the algae dried in step (2') is blended as a raw material for feed.

[0126] The amount of Nori seaweed to be added is not particularly limited as long as it can suppress methane derived from livestock burps, and may be, for example, 0.1% to 50.0% by mass, 0.5% to 30.0% by mass, or 1.0% to 10.0% by mass of the total feed. The amount of Nori seaweed to be added can be changed as appropriate depending on the type, size, sex, etc. of the livestock.

[0127] The method for producing a feed according to this embodiment may, if necessary, include a step of crushing the dried Porphyra sieboldii before step (3'). By including this step, the method for producing a feed according to this embodiment can uniformly blend Porphyra sieboldii in step (3').

[0128] Furthermore, the method for producing a feed according to this embodiment may, if necessary, include a molding step after step (3') for molding the feed containing Porphyra laver into pellets or the like. By including this step, the method for producing a feed according to this embodiment can produce feed in a size that is easy for livestock to eat.

[0129] The feed manufacturing method of this embodiment is a method for manufacturing feed for livestock, and by including the above steps (1') to (3'), large amounts of algae can be obtained efficiently, and therefore feed containing algae can be manufactured efficiently and in large quantities.

[0130] The method for cultivating Porphyra japonica and the method for producing feed according to the present invention are not limited to the above-described embodiments, and various modifications are possible within the scope of the present invention.

[0131] The present invention includes the following aspects. [1] A method for culturing Porphyra japonica using a culture plate, the culture plate has a plate body and a plurality of second protrusions protruding from one surface side of the plate body; A method for cultivating Porphyra japonica, comprising the following steps (1) to (3): Step (1): Culturing sporophytes on which tetrasporangia have been formed using a culture plate Step (2): Culturing young gametophytes using the culture plate on which the sporophytes on which tetrasporangia have been formed in step (1) have been cultured. Step (3): Culturing and recovering gametophytes in the sea using the culture plate on which the juvenile gametophytes were cultured in step (2). [2] Step (1) Step (1-1): placing a first culture plate in a first water tank; Step (1-2): A step of introducing a gametophyte having a carposporophyte into a first tank, thereby releasing carpospores from the carposporophyte into the first tank; and Step (1-3): A step of implanting the carpospores between adjacent second protrusions of the first culture plate, thereby forming sporophytes from the implanted carpospores, and growing the sporophytes until tetrasporangia are formed, thereby culturing the sporophytes with tetrasporangia formed; Including, Step (2) is Step (2-1): Recovering a first culture plate having sporangia formed thereon from a first water tank and placing the first culture plate in a second water tank; and Step (2-2): A step of implanting the tetraspores released from the tetrasporangia between adjacent second protrusions of a second culture plate placed in a second water tank, thereby forming juvenile gametophytes from the tetraspores and allowing the stolon of the juvenile gametophyte and the rhizoid threads extending from the stolon to attach to the second protrusions, thereby culturing the juvenile gametophyte; Including, Step (3) is Step (3-1): recovering the second culture plate to which the juvenile gametophytes are attached from the second tank and submerging it in the sea, thereby culturing the gametophytes; and Step (3-2): recovering the cultured gametophytes together with the second culture plate from the sea; The method for cultivating Porphyra japonica according to [1], comprising: [3] The method for cultivating Porphyra japonica according to [1] or [2], wherein steps (1) to (3) are carried out at a water temperature of 20°C or higher and 35°C or lower. [4] A method for cultivating Porphyra japonica described in [2], wherein the gametophyte having the carposporophyte in step (1-2) is derived from a recovered gametophyte. [5] A method for cultivating Porphyra japonica described in any one of [1] to [4], wherein the distance between adjacent second protrusions in the plurality of second protrusions is 0.1 mm or more and 5.0 mm or less. [6] The method for cultivating Undaria pinnatifida according to any one of [1] to [5], wherein the second protrusion is cylindrical and has a diameter of 1.0 mm or more and 5.0 mm or less. [7] A method for producing livestock feed, comprising the following steps (1') to (3'): Step (1'): A step of culturing Uncaria japonica using the method for cultivating Uncaria japonica according to any one of [1] to [6]. Step (2'): A step of drying the algae cultured in step (1'). Step (3'): A step of incorporating the dried algae obtained in step (2') as a raw material for feed. [Explanation of symbols]

[0132] 2. Culture Plates 21 Plate body 22 1st protrusion 23 Second protrusion 24 Convex part 25 holes 26 Cut 27 volts 3. Concrete blocks 31 Convex part 4 Seaweed Reef 5. Pedestal 6. Fence 7 Hook A. Gametophyte A1 Rhizoid thread extending from the stolon of the gametophyte B. Seed threads of algae L: A line connecting the centers of the opposing long sides of the four edges of the plate body. S One side of the plate body S1 Placement of the Kagikenori S' The other side of the plate body T concrete block surface

Claims

1. A method for culturing Porphyra japonica using a culture plate, comprising: The culture plate has a plate body and a plurality of second protrusions protruding from one surface side of the plate body, A method for cultivating Kagikenori, comprising the following steps (1) to (3). Step (1): Culturing sporophytes on which tetrasporangia have been formed using a culture plate Step (2): Culturing young gametophytes using the culture plate on which the sporophytes on which tetrasporangia have been formed in step (1) have been cultured. Step (3): Cultivating and recovering gametophytes in the sea using the culture plate on which the juvenile gametophytes were cultured in step (2).

2. Step (1) is Step (1-1): placing a first culture plate in a first water tank; Step (1-2): A step of introducing a gametophyte having a carposporophyte into a first tank, thereby releasing carpospores from the carposporophyte into the first tank; and Step (1-3): A step of implanting the carpospores between adjacent second protrusions of the first culture plate, thereby forming sporophytes from the implanted carpospores, and growing the sporophytes until tetrasporangia are formed, thereby culturing the sporophytes with tetrasporangia formed; Including, Step (2) is Step (2-1): Recovering a first culture plate having sporangia formed thereon from a first water tank and placing the first culture plate in a second water tank; and Step (2-2): A step of implanting the tetraspores released from the tetrasporangia between adjacent second protrusions of a second culture plate placed in a second water tank, thereby forming juvenile gametophytes from the tetraspores and fixing the stolon of the juvenile gametophyte and the rhizoid threads extending from the stolon to the second protrusions, thereby culturing the juvenile gametophyte; Including, Step (3) is Step (3-1): A step of recovering the second culture plate to which the juvenile gametophytes are attached from the second tank and submerging it in the sea to culture the gametophytes; and Step (3-2): recovering the cultured gametophytes together with the second culture plate from the sea; The method for cultivating Porphyra japonica according to claim 1, comprising:

3. The method for cultivating Porphyra japonica according to claim 1, wherein steps (1) to (3) are carried out at a water temperature of 20°C or higher and 35°C or lower.

4. The method for cultivating Porphyra japonica according to claim 2, wherein the gametophyte having the carposporophyte in step (1-2) is derived from a recovered gametophyte.

5. The method for cultivating algae according to claim 1, wherein the distance between adjacent second protrusions in the plurality of second protrusions is 0.1 mm or more and 5.0 mm or less.

6. 2. The method for cultivating algae according to claim 1, wherein the second protrusion has a cylindrical shape and a diameter of 1.0 mm or more and 5.0 mm or less.

7. A method for producing livestock feed, comprising the following steps (1') to (3'): Step (1'): A step of culturing Porphyra japonica using the method for culturing Porphyra japonica according to any one of claims 1 to 6. Step (2'): A step of drying the algae cultured in step (1'). Step (3'): A step of incorporating the algae dried in step (2') as a raw material for feed.

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