Hair moisture locking and repair composition and its use

Abstract

A hair moisture lock and repair composition and its use, wherein the functional components of this composition are the filtrate or lysate of yeast fermentation products and composite hyaluronic acid or its salts. The content of the filtrate or lysate of yeast fermentation products is 1 to 90% by weight, and the content of composite hyaluronic acid or its salts is 1 to 20% by weight. Compared with single components, this composition has a synergistic effect in aspects such as improving the strength and toughness of hair, preventing split ends, and preventing frizz, and has prospects for wide application in the fields of hair washing and hair care.

Description

Technical Field

[0001] The present invention relates to a hair moisture lock and repair composition and its use, and particularly to a hair moisture lock and repair composition having the effects of improving hair strength and toughness, preventing split ends, and preventing shrinkage, and its use, belonging to the technical field of hair products.

Background Art

[0002] Today, people's demand for beauty is increasing day by day, and the frequency of washing, perming, dyeing, and styling hair is increasing. Furthermore, environmental stress is likely to damage hair, and due to these factors, hair often becomes dry and shrinks, its flexibility decreases, the hair becomes damaged, and it is easily broken. Therefore, the development of hair conditioning and effective ingredients and products with repair effects has become a research hot spot. Currently, there are the following three most widely used hair repair effective ingredients. 1) Cationic polymer conditioners such as polyquaternium salts and cationic modified polysaccharides are common hair conditioners that can improve hair flexibility and repair the surface hair scales. 2) Proteins, hydrolyzed proteins, and polypeptide polymers have the effect of repairing damaged hair, improving the mechanical strength of hair, and reducing split ends. 3) Small molecule moisturizing ingredients such as panthenol, amino acids, and organic molecular acids have also been proven to have the effect of confining moisture and giving moisture to hair, improving hair quality.

[0003] Hyaluronic acid (HA) is a high molecular weight bioactive substance that binds to a large number of water molecules through hydrogen bonds and has the effects of moisturizing and retaining moisture. In recent years, it has been widely used in skin care products. China's technology for producing hyaluronic acid by microbial fermentation is in a leading position in the world. In recent years, local enterprises have mainly produced oligomeric hyaluronic acid using enzyme digestion technology, and the functions such as deep moisturizing, anti-inflammatory, sedative, and repair of hyaluronic acid with different molecular weights have also been expanded. However, so far, the application of hyaluronic acid, especially oligomeric hyaluronic acid, in the field of hair care technology is still relatively few.

Disclosure of the Invention

[0004] The object of the present invention is to provide a hair moisture locking and repair composition comprising a filtrate or solution of a yeast fermentation product and a complex hyaluronic acid or a salt thereof. During experiments, it was unexpectedly found that, compared to a single component, there is a synergistic effect in terms of improving hair strength and toughness, preventing breakage, and preventing frizz, and it has the potential for a wide range of applications in the fields of shampooing and hair care.

[0005] The present invention provides a hair moisture locking and repair composition in which the functional components are a filtrate or solution of a yeast fermentation product and a complex hyaluronic acid or a salt thereof, wherein the content of the filtrate or solution of the yeast fermentation product is 1 to 90% by weight, the content of the complex hyaluronic acid or a salt thereof is 1 to 20% by weight, and the total content of both is 100% by weight or less.

[0006] Furthermore, in the above-mentioned hair moisture locking and repair composition, the content of the filtrate or solvent of the yeast fermentation product is preferably 5 to 50% by weight, and more preferably 5 to 30% by weight.

[0007] Furthermore, in the above-mentioned hair moisture locking and repair composition, the content of complex hyaluronic acid or its salt is 1 to 15% by weight, more preferably 1 to 10% by weight.

[0008] For example, the content of the filtrate or lysate of the yeast fermentation product may be 1% by weight, 5% by weight, 10% by weight, 15% by weight, 20% by weight, 25% by weight, 30% by weight, 35% by weight, 40% by weight, 45% by weight, 50% by weight, 55% by weight, 60% by weight, 65% by weight, 70% by weight, 75% by weight, 80% by weight, 85% by weight, 90% by weight, etc. The content of the complex hyaluronic acid or its salt may be 1% by weight, 2% by weight, 3% by weight, 4% by weight, 5% by weight, 6% by weight, 7% by weight, 8% by weight, 9% by weight, 10% by weight, 11% by weight, 12% by weight, 13% by weight, 14% by weight, 15% by weight, 16% by weight, 17% by weight, 18% by weight, 19% by weight, 20% by weight, etc.

[0009] Furthermore, the content ratio of the filtrate or lysate of the yeast fermentation product to the complex hyaluronic acid or its salt is 1:4 to 9:1, preferably 1:3 to 2:1.

[0010] For example, the content ratio of the filtrate or lysate of the yeast fermentation product to the complex hyaluronic acid or its salt may be 1:4, 1:3.5, 1:3, 1:2.5, 1:2, 1:1.5, 1:1, 1.5:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, etc.

[0011] Furthermore, in the above-mentioned hair moisture locking and repair composition, water may be included as a solvent in addition to the functional ingredients, and the remaining water content can be up to 100% by weight.

[0012] Furthermore, the filtrate or lysate of the yeast fermentation product described in the present invention refers to the fermentation filtrate or lysate obtained by fermenting plants such as rice, beans, or wheat with yeast. For example, the fermentation filtrate is the fermentation product obtained by the growth and / or fermentation of yeast in a culture medium containing rice, beans, or wheat. The filtrate of the fermentation product is obtained by filtering the fermentation product, and the intracellular substances obtained by crushing the microbial cells in the fermentation product are the lysate of the fermentation product. In addition to rice, beans, or wheat, one or more components necessary for fermentation, such as a carbon source (sugars), a nitrogen source (yeast powder, peptone, etc.), and inorganic salts, can be added to the culture medium as needed for fermentation.

[0013] Preferably, the filtrate or soluble solution of the yeast fermentation product is a fermentation filtrate or soluble solution obtained by fermenting rice, especially brown rice (whole grain rice), with yeast, and can be called a yeast / rice fermentation product filtrate or a yeast / rice fermentation soluble solution. The yeast / rice fermentation product filtrate is prepared by germinating brown rice, grinding it into brown rice powder, making rice milk from it, enzymatically hydrolyzing it, adding yeast and fermenting it, and then removing impurities and bacteria from the fermentation liquid.

[0014] Furthermore, the complex hyaluronic acid or its salt is a composition of oligomeric hyaluronic acid or its salt and low molecular weight hyaluronic acid or its salt, where the molecular weight of the oligomeric hyaluronic acid or its salt is 8 kDa or less, preferably 5 kDa or less, and the molecular weight of the low molecular weight hyaluronic acid or its salt is 20 kDa to 80 kDa, preferably 30 kDa to 50 kDa.

[0015] Furthermore, the mass ratio of oligomeric hyaluronic acid or its salt to low molecular weight hyaluronic acid or its salt is 1:5 to 5:1, preferably 1:4 to 3:2.

[0016] For example, the mass ratio of oligomeric hyaluronic acid or its salt to low molecular weight hyaluronic acid or its salt may be 1:5, 1:4.5, 1:4, 1:3.5, 1:3, 1:2.5, 1:2, 1:1.5, 1:1, 1.5:1, 2:1, 3:1, 4:1, 5:1, etc.

[0017] Furthermore, salts of oligomeric hyaluronic acid or low molecular weight hyaluronic acid include sodium salts, potassium salts, calcium salts, zinc salts, magnesium salts, and the like.

[0018] The hair moisture locking and repair composition of the present invention exhibits unexpected synergistic effects in terms of improving hair strength and toughness, preventing breakage, and preventing frizz, and has good prospects for application in hair products. Here, the hair product may be a hair care product or a shampoo product, and the hair product may be a rinse-off type or a leave-in type.

[0019] The present invention also provides a hair product in which the functional ingredients of the hair product include the above-described hair moisture locking and repair composition of the present invention.

[0020] Furthermore, the hair product described above may also contain other functional ingredients, such as peptides, panthenol, and amino acids, that have functions such as moisturizing, hydrating, moisture locking, repairing, cleansing, preventing dandruff, and preventing hair loss, in addition to the hair moisture locking and repairing composition of the present invention.

[0021] Furthermore, the content of the above-mentioned hair moisture locking and repair composition in the hair product is 0.5 to 10% by weight, preferably 1 to 5% by weight, and more preferably 2 to 3% by weight.

[0022] For example, the content of the above-mentioned hair moisture locking and repair composition in a hair product may be 0.5% by weight, 1% by weight, 1.5% by weight, 2% by weight, 2.5% by weight, 3% by weight, 3.5% by weight, 4% by weight, 4.5% by weight, 5% by weight, 5.5% by weight, 6% by weight, 6.5% by weight, 7% by weight, 7.5% by weight, 8% by weight, 8.5% by weight, 9% by weight, 9.5% by weight, 10% by weight, etc.

[0023] Furthermore, the above-mentioned hair products may be hair care products or shampoo products, and the hair products may be rinse-off or leave-in types.

[0024] Furthermore, the hair care products mentioned above include various types such as shampoos, conditioners, hair masks, and sprays.

[0025] Furthermore, the above-mentioned hair products may contain additional auxiliary components necessary for manufacturing different dosage forms, each of which may be selected from existing technologies, and the method for preparing the dosage forms may also be carried out in accordance with existing technologies.

[0026] In the present invention, a filtrate or lysate of a yeast fermentation product, an oligomeric hyaluronic acid or its salt, and a low molecular weight hyaluronic acid or its salt are combined. The filtrate or lysate of the yeast fermentation product contains abundant amino acids, polypeptides, minerals, and other active substances, which can penetrate deeply into the core of hair, repair damaged chemical bonds, and have the effect of repairing keratin. By blending hyaluronic acids with different molecular weights, effective penetration and distribution into hair can be achieved, a three-dimensional repair and moisture locking effect can be realized, the hydrogen bonds inside the hair can be strengthened, and the strengthening of the keratin structure can be realized. As a result, the strength and toughness of the hair are effectively improved, and the hair is repaired and / or protected from damage to the mechanical properties of the hair caused by various damage stresses (such as bleaching, perming and dyeing, exposure to pollution, ultraviolet irradiation, multiple washings, high temperature, etc.). In addition, this composition also has a lubricating effect and an anti-frizz effect on the surface of the hair, and can improve the performance of hair care products and hair washing products.

Brief Description of the Drawings

[0027] [Figure 1] It is a fluorescence microscope photograph of fluorescein-labeled sodium hyaluronate penetrated into hair. A is sodium hyaluronate with a molecular weight of 30 - 50KDa, and B is high molecular weight sodium hyaluronate with a molecular weight of 1460kDa. [Figure 2] It is a figure showing the strength and toughness repair effect of the composition of the present invention on damaged hair and healthy hair under various conditions. [Figure 3] It is a figure showing the anti-frizz effect of the composition of Example 1 of the present invention on hair. Details of the Invention

[0028] In order to make the above objects, features and advantages of the present invention clearer and easier to understand, specific embodiments of the present invention will be described in detail below with reference to specific examples and drawings.

[0029] In the following examples, the INCI name of the yeast / rice fermentation product filtrate used was SACCHAROMYCES / RICE FERMENT FILTRATE, and the raw material used was a commercially available product with the trade name: Brown Rice Fermentation Filtrate (Bioyouth®-Brice). The Lactobacillus rye flour ferment was a commercially available product. The hyaluronic acid used was all from Bloomage Biotechnology Corporation Limited.

[0030] Test Example 1: Tensile Strength Test Each ingredient was weighed according to the formulation shown in Table 1 below, added to water, and mixed uniformly to obtain a hair moisture lock and repair composition.

[0031] [Table 1]

[0032] The hair moisture locking and repair composition prepared as described above was subjected to a tensile strength test. The test method was as follows:

[0033] First, the compositions prepared in each example and comparative example were diluted with deionized water. The aqueous solutions of the compositions other than those in Example 2 were then diluted with water so that the active ingredient content in each composition solution was 2.5% by weight. The prepared solutions were placed in spray bottles or pump bottles and prepared as test samples.

[0034] In Comparative Example 5, the high viscosity of 1460 kDa sodium hyaluronate prevented the preparation of a 10% solution. Therefore, a 2% solution was prepared, and the amount of yeast / rice fermentation product filtrate added was simultaneously adjusted to 2%.

[0035] Thirteen severely damaged hair bundles (multiple bleached hair bundles, 15 cm long, 1 g in weight) were selected. All bundles were pre-shampooed with 10% SDS and air-dried under constant temperature and humidity conditions of (25 ± 2) °C and (50 ± 5)%. After the hair bundles were completely dry, following the instructions for using hair care spray, an equal amount (0.3 g) of the test sample for each example and comparative example was sprayed onto 12 hair bundles, and the same amount (0.3 g) of water was sprayed onto one hair bundle. The bundles were then air-dried under constant temperature and humidity conditions, and the above procedure was repeated 10 times. After the final procedure and drying, all hair bundles in each group were washed with deionized water to remove any remaining sample from the surface of the hair bundles, and then dried in a constant temperature and humidity environment. The diameter of the hair was measured using a high-definition camera SN-1200W. Specifically, the diameter was measured at three points in the center of the hair and the average value was taken. From each of the 13 hair bundles, 30 hairs with a diameter difference of 10 μm or less were taken, and single-fiber strength tests were performed using a fiber strength tester. The tensile strength of the hair in the control group and each sample group was calculated and compared using the following formula. σ = Fb / So In the formula, σ is the tensile strength, Fb is the maximum force the sample experienced when it fractured, and So is the original cross-sectional area of ​​the sample. The average modulus of hair was also calculated from the modulus region of the tensile curve.

[0036] The results of the hair tensile strength test are shown in Table 2 below.

[0037] [Table 2]

[0038] The results in the table above show that the tensile strength of Examples 1-7 was higher than that of the blank control, indicating that the combination of yeast / rice fermentation product filtrate and sodium hyaluronate is indeed beneficial in improving hair strength and toughness.

[0039] The tensile strength of Example 1 was significantly higher than that of Comparative Examples 1 and 2 and the blank control, demonstrating that the product combining yeast / rice fermentation product filtrate with two molecular weight sodium hyaluronates contributed more to improving hair strength and toughness than the product combining yeast / rice fermentation product filtrate with a single sodium hyaluronate, thus proving the necessity of incorporating two molecular weight sodium hyaluronates. The tensile strength results of Examples 1-7 indicate that the improvement in hair strength and toughness becomes more pronounced as the ratio of yeast / rice fermentation product filtrate to two molecular weight sodium hyaluronates approaches 1:1.

[0040] At the same time, the tensile strength of Example 1 was also higher than that of Comparative Examples 3 and 4. This indicates that the combination of yeast / rice fermentation product filtrate and two types of sodium hyaluronate in the composition has a synergistic effect on improving hair strength and toughness, and that there is no clear synergistic effect when other types or varieties of fermentation product filtrates are used instead. The tensile strength of Example 1 and Comparative Examples 1 and 2 were significantly higher than that of Comparative Example 5, indicating that the selection of the molecular weight of hyaluronic acid greatly influences the improvement of hair strength and toughness.

[0041] Figure 1 shows the results of an experiment on the penetration of fluorescein-labeled sodium hyaluronate into hair. While high molecular weight sodium hyaluronate (1460 kDa) does not penetrate, sodium hyaluronate with a molecular weight of 30-50 kDa penetrates the hair, even the hair shaft, and ultimately the core of the hair, exhibiting a repair effect. The specific experimental procedure is as follows.

[0042] 0.2 g of sodium hyaluronate was taken, 2 mL of 0.05 mol / L NaOH aqueous solution was added to a stoppered test tube, the tube was stoppered, and the sodium hyaluronate was dissolved by vortexing. 0.04 g of FITC was precisely added, the tube was stoppered and sealed, and thoroughly mixed with a vortex mixer. After 45 minutes of constant temperature reaction in a 95°C water bath, the tube was removed, cooled to room temperature, 18 mL of sodium chloride saturated anhydrous ethanol was added, and the supernatant was discarded. The resulting precipitate was crude FITC fluorescently labeled sodium hyaluronate. 20 mL of sodium chloride saturated anhydrous ethanol was added to the precipitate and vortexed to uniformly disperse the labeled sodium hyaluronate precipitate in the sodium chloride saturated anhydrous ethanol. The upper layer of alcohol washing solution was discarded after centrifugation; this constituted one alcohol wash. After repeating this alcohol wash six times, the precipitate was freeze-dried to obtain the sodium hyaluronate-FITC fluorescent marker. Experiments were conducted on severely damaged hair. 0.05 g of sodium hyaluronate-FITC fluorescent marker was accurately weighed into a stoppered test tube, 10 mL of deionized water was added, the tube was sealed, and dissolved by vortexing. Twenty strands of hair were randomly selected and immersed in the fluorescently labeled sodium hyaluronate solution for 4 hours. After immersion, the hair strands were removed, and the surface was thoroughly washed to remove any remaining sodium hyaluronate. Cross-sections of the hair were then obtained using frozen sections and observed using a fluorescence microscope. As can be seen in the figures, 1460 kDa fluorescently labeled polymeric sodium hyaluronate (right in Figure 1) showed very little penetration into the hair, while fluorescently labeled sodium hyaluronate of 30-50 kDa (left in Figure 1) showed significant fluorescence in the hair, and even in the core of the hair.

[0043] Further analysis of the tensile curve properties obtained in each experiment leads to the conclusion that the elastic modulus and elongation at break of the hair differ when different test samples are used in the test. The elastic modulus and elongation at break of some test samples are shown in Table 3.

[0044] [Table 3]

[0045] The above results indicate that the effect of the yeast / rice fermentation product filtrate on the mechanical properties of hair differs when combined with different hyaluronic acids. When the yeast / rice fermentation product filtrate is combined with oligomeric sodium hyaluronate (Comparative Example 1), the elongation at break of the hair can be significantly improved, but the elastic modulus of the hair has little effect. When the yeast / rice fermentation product filtrate is combined with low molecular weight sodium hyaluronate (Comparative Example 2), the elastic modulus of the hair can be significantly improved, but the elongation at break of the hair has little effect. When the yeast / rice fermentation product filtrate is combined with two types of sodium hyaluronate, both the elastic modulus of the hair and the elongation at break can be significantly improved simultaneously (Example 1). Therefore, it is necessary to combine two types of sodium hyaluronate in the composition. The principle is as follows. Low molecular weight sodium hyaluronate and oligomeric sodium hyaluronate have complementary effects in increasing hair strength and toughness. Low molecular weight sodium hyaluronate is more effective in increasing the elastic modulus of hair, while oligomeric sodium hyaluronate is more effective in increasing the elongation of hair at break. This is likely because low molecular weight sodium hyaluronate has a strong moisture-locking effect and is superior in strengthening the hydrogen bond network. On the other hand, oligomeric sodium hyaluronate is more active and permeable, resulting in a stronger interaction with keratin.

[0046] Test Example 2: Antioxidant Activity Test The filtrate of the yeast / rice fermentation product is rich in active ingredients such as peptides, amino acids, and antioxidants like phytic acid, ferulic acid, lactic acid, phytoamides, and sterols. These small molecule active ingredients have high permeability and, in addition to improving the hair moisture-locking properties, strength, and toughness of the composition, also possess antioxidant and free radical scavenging effects. Since hair generates oxidative free radicals under UV irradiation, which further damages the hair structure and reduces hair strength and toughness, the yeast / rice fermentation product filtrate in the composition also provides the composition with the ability to protect hair from UV damage.

[0047] To verify this antioxidant property, the hair moisture locking and repair composition solutions of Example 1, Comparative Example 3, and Comparative Example 4 were diluted with deionized water until the hair moisture locking and repair composition was 2.5% by weight, and test samples were prepared. The hair treatment method was as follows: Healthy hair bundles were selected, washed with 10% SDS, and then stored under constant temperature and humidity conditions of (25±2)°C and (50±5)% humidity, and each hair bundle was treated as shown in Table 4. Here, ultraviolet irradiation was performed in a xenon lamp aging test chamber, and after 24 hours of irradiation, an equal amount of sample was sprayed onto the hair bundles. The tensile strength of the treated hair was tested according to the method of Test Example 1.

[0048] Measurement of DPPH clearance rate: 2 mL of 2 mmol / L DPPH solution was used as the blank group, 2 mL of anhydrous ethanol was used as the control group instead of DPPH solution, and 2 mL of anhydrous ethanol and 2 mL of the samples from Example 1, Comparative Example 3, and Comparative Example 4, diluted to a composition concentration of 2.5 wt%, were used as the sample group. The mixtures were thoroughly shaken to ensure uniformity, reacted in the dark for 30 minutes, and the absorbance value (D value) at 517 nm was measured. The above experiment was repeated three times. The clearance rate was calculated, and the antioxidant activity of each sample was analyzed. Clearance rate (%) = [1 - (D experiment - D blank) / D control] × 100%.

[0049] The experimental results are shown in Table 4 below.

[0050] [Table 4]

[0051] As can be seen from the data above, healthy hair that was not treated with any product showed a 19.0% decrease in strength and toughness after UV irradiation. However, after treatment with the composition of Example 1, the decrease in strength and toughness after further UV irradiation was significantly smaller, and clearly smaller than that of Comparative Examples 3 and 4. This indicates that the composition of Example 1 has UV protection properties for hair, and that the combination of the yeast / rice fermentation product filtrate and the complex hyaluronic acid of this application has a synergistic effect on antioxidants.

[0052] Test Example 3: Comb Passability Test The experiment was conducted under conditions of a temperature of (25±2)°C and a humidity of (50±5)%. A human hairpiece measuring 40 cm in length and 25 g in mass was selected. The hairpiece was thoroughly wet with 40°C warm water, 5 mL of 10% SDS aqueous solution was pipetted onto both sides of the hairpiece, and it was rubbed to create lather. Care was taken not to bend or twist the hair strands to prevent tangling, and the lather was rinsed off with warm water. The above procedure was repeated twice, and the hairpiece was then allowed to air dry in a constant temperature and humidity environment.

[0053] The hair moisture locking and repair compositions of Example 1 and Comparative Examples 1-3 were all diluted with deionized water to a concentration of 2.5% by weight to prepare test samples.

[0054] 1.5 g of the test sample prepared in each example and comparative example was accurately weighed, uniformly sprayed onto a hair bundle, and then lightly combed with a comb. Clean water was used as a blank control. After the hair bundles were naturally dried at a constant temperature and humidity, the combability of each hair bundle in its dry state was tested using a combing device. Two hairpieces of the same specifications were tested, and the test was repeated seven times. A combability curve was obtained by data processing, and the combing work could be obtained by integrating this combability curve. By comparing the combing work before and after use, the ability of the sample to improve the combability of the hair bundles could be obtained.

[0055] The experimental results are shown in Table 5 below.

[0056] [Table 5]

[0057] The results from Comparative Examples 1 and 2 and the blank control in the table above show that a combination of single hyaluronic acid and yeast / rice fermentation product filtrate can reduce the combability of hair. The comparison between Example 1 and Comparative Examples 1 and 2 shows that the combination of complex hyaluronic acid and yeast / rice fermentation filtrate is more effective at reducing combability than the combination of single molecular weight hyaluronic acid and yeast / rice fermentation filtrate. Furthermore, the results from Example 1 and Comparative Example 3 show that the combination of complex hyaluronic acid and yeast / rice fermentation filtrate is more effective at reducing combability than the combination of complex hyaluronic acid and other fermentation filtrates. This indicates that the composition has an optimized technical effect in improving the combability of hair strands. This is because the composition contains active substances of different molecular weights, which can form a dense and effective lubricating film on the hair surface, thus moisturizing the hair surface. In daily life, hair that is difficult to comb and easily tangles is a major cause of breakage during combing, so improving combability is extremely important to prevent breakage. Therefore, the composition of the present invention not only improves the strength and toughness of individual hair fibers, but also improves the combability of hair bundles, thereby comprehensively achieving the technical effect of preventing hair breakage.

[0058] Test Example 4: Tensile Strength Tests on Hair Textures under Various Conditions The damaged hair used in the aforementioned experiment was hair damaged by multiple bleaching treatments. In daily life, hair is subjected to stress from various situations, such as hair dyeing, chemical perms, exposure to pollutants, UV radiation, multiple washes, and heat straightening treatments.

[0059] To test the changes in strength and toughness when the composition of Example 1 of the present invention is applied to various types of damaged hair, the hair moisture lock and repair composition solution of Example 1 was diluted with water to create a test sample with a concentration of 2.5% by weight of the hair moisture lock and repair composition. Following the method of Test Example 1, the test samples were used on different types of damaged hair, and the tensile strength of the hair was tested before and after use. Clean water was used as a blank control. The experimental results are shown in Figure 2. From the figure, it can be seen that the composition of the present invention is suitable for repairing the strength and toughness of various types of damaged hair, and can also improve the strength and toughness of healthy hair.

[0060] Test Example 5: Anti-waviness and frizz performance The hair moisture locking and repair composition of Example 1 was diluted with water to prepare a test sample with a concentration of 2.5% by weight.

[0061] Frizz prevention test: Six hair bundles (3 healthy, 3 damaged, all 15cm long, 1g in weight) were pre-shampooed with 10% SDS and air-dried under constant temperature and humidity conditions of (25±2)°C and (50±5)%. After the hair bundles were completely dry, 0.3g of deionized water and the test sample were sprayed onto each of the six hair bundles, and they were air-dried under constant temperature and humidity conditions. Then they were placed in an environment with approximately 80% humidity (simulating a rainy day), and photographs were taken at 0 hours and 4 hours. By comparing the degree of waviness and frizz of the hair bundles and the change in volume of the hair bundles under high humidity conditions, the product's frizz prevention performance can be determined.

[0062] The experimental results are shown in Figure 3. Figure 3a shows healthy hair bundles before high humidity environment treatment (for before-and-after comparison), Figure 3b is an image of healthy hair bundles after treatment with the test sample and standing for 4 hours under high humidity of 80%, and Figure 3c is an image of healthy hair bundles after treatment with deionized water and standing for 4 hours under high humidity of 80%. Figure 3e shows damaged hair bundles before high humidity environment treatment (for before-and-after comparison), Figure 3d is an image of damaged hair bundles after treatment with the test sample and standing for 4 hours under high humidity of 80%, and Figure 3f is an image of damaged hair bundles after treatment with deionized water and standing for 4 hours under high humidity of 80%. From Figure 3, it can be seen that the volume of hair bundles not treated with the test sample clearly increased in a humid environment, and the hair bundles, especially the ends, became slightly wavy and curled, but the volume of hair bundles treated with the test sample showed little change under high humidity conditions. Therefore, the composition of the present invention also has the effect of preventing hair frizz and waviness, and can prevent hair from becoming difficult to manage under high humidity conditions.

[0063] Test Example 6: Hair Moisture Locking Effect Test Hair contains both free water and bound water, with free water having a significant impact on hair properties. Excessive free water often causes the breakdown of hydrogen bonds in hair, reducing the crystallinity of keratin and consequently decreasing strength and toughness. The composition of the present invention combines sodium hyaluronate and yeast / rice fermentation product filtrate with different molecular weights. The molecular weights of the water-locking components range from tens of thousands to hundreds and contain abundant moisturizing groups (carboxyl groups, amino groups, hydroxyl groups). This achieves a three-dimensional water-locking effect, strengthens the hydrogen bonding effect within the hair, reinforces the keratin structure, and ultimately results in improved hair strength and toughness.

[0064] The test method for the hair moisture lock effect is as follows. The experiment was conducted in an environment with a temperature of (25±2)°C and a humidity of 50±5%. Six bundles of human hairpieces, each 40 cm long and weighing 25 g, were taken, pre-shampooed with 10% SDS, and then allowed to air dry for 24 hours in a constant temperature and humidity environment. First, a certain amount of hair bundles were taken from each group and placed in a test moisture meter set to a temperature of 65°C and a test time of 20 minutes to measure the moisture loss rate at 65°C (this reflects the free moisture content of the hair, excluding structured water). Next, 1 g of deionized water and 1 g of 0.5% hair moisture lock / repair composition diluent were accurately measured and uniformly sprayed onto the hair bundles, respectively. After drying for 12 hours in a constant temperature and humidity environment, the hair bundles were rinsed to remove any remaining samples. After repeating the above procedure 10 times, a certain amount of hair bundle was taken, placed in a moisture meter, and the moisture loss rate at 65°C was measured. The heat-gravity loss of the hair during this process was recorded, and the free moisture content in the hair was obtained. The experimental results are shown in Table 6 below.

[0065] [Table 6]

[0066] The results in the table above show that Example 1 has the most significant effect in reducing the free water content in the hair. This indicates that the composition of the present invention has the best hair moisture locking effect, and that this moisture locking effect is the important reason for improving the strength and toughness of the hair and producing the technical effects of preventing frizz and waviness.

[0067] Test Example 7: Screening of the content of hair moisture locking and repair composition The hair moisture locking and repair composition of Example 1 was diluted with deionized water so that the content of the active ingredient of the hair moisture locking and repair composition was as shown in Table 7 below. The hair tensile strength was measured for aqueous solutions with different mass ratios of the obtained hair moisture locking and repair composition according to the method of Test Example 1. The results are shown in Table 7 below.

[0068] [Table 7]

[0069] From the results above, it can be seen that in actual use, when this composition was added at a concentration of 0.1%, no clear improvement in hair strength and toughness was observed, but when added at concentrations of 0.5% to 10%, hair strength and toughness could be significantly improved in all cases. Therefore, the preferred concentration of this composition in hair products is 0.5 to 10%. Furthermore, a concentration of 1 to 5% is more preferable, and 2 to 3% is even more preferable.

[0070] Test Example 8: Screening of the mixing ratio of oligomeric sodium hyaluronate and low molecular weight sodium hyaluronate. Following the method of Example 1, yeast / rice fermentation product filtrate, oligomeric sodium hyaluronate, low molecular weight sodium hyaluronate, and water were mixed to form a hair moisture lock and repair composition solution with a yeast / rice fermentation product filtrate content of 10% by weight and a total content of oligomeric sodium hyaluronate and low molecular weight sodium hyaluronate of 10% by weight. By changing the mass ratio of oligomeric sodium hyaluronate and low molecular weight sodium hyaluronate, multiple groups of hair moisture lock and repair composition solutions were obtained as shown in Table 8 below. Each group of hair moisture lock and repair composition solution was diluted with water to obtain test samples with a hair moisture lock and repair composition content of 2.5% by weight. The hair tensile strength of the test samples was tested according to the method of Test Example 1. The test results are shown in Table 8 below.

[0071] [Table 8]

[0072] As can be seen from the experimental results above, when the ratio of oligomeric sodium hyaluronate to low molecular weight sodium hyaluronate is in the range of 1:5 to 5:1, the strength and toughness of the hair can be effectively improved, showing a good synergistic effect. Here, when the ratio of oligomeric sodium hyaluronate to low molecular weight sodium hyaluronate is in the range of 3:2 to 1:4, the increase in strength and toughness exceeds 20%, so a mass ratio of oligomeric sodium hyaluronate to low molecular weight sodium hyaluronate of 3:2 to 1:4 is preferable.

Claims

1. The functional component comprises a filtrate or lysate of a yeast fermentation product and a complex hyaluronic acid or a salt thereof, wherein the content of the filtrate or lysate of the yeast fermentation product is 1 to 90% by weight, and the content of the complex hyaluronic acid or a salt thereof is 1 to 20% by weight. The filtrate or soluble solution of the yeast fermentation product is a fermentation filtrate or soluble solution obtained by fermenting rice, legumes, or wheat with yeast. The aforementioned complex hyaluronic acid or its salt is a composition of oligomeric hyaluronic acid or its salt and low molecular weight hyaluronic acid or its salt. A hair moisture locking and repair composition characterized in that the molecular weight of the oligomeric hyaluronic acid or its salt is 8 kDa or less, and the molecular weight of the low molecular weight hyaluronic acid or its salt is 20 kDa to 80 kD.

2. The hair moisture locking and repair composition according to claim 1, characterized in that the content of the filtrate or solution of the yeast fermentation product is 5 to 50% by weight, and the content of the complex hyaluronic acid or a salt thereof is 1 to 15% by weight.

3. The hair moisture locking and repair composition according to Claim 1, characterized in that the content of the filtrate or solubility of the yeast fermentation product is 5 to 30% by weight, and the content of the complex hyaluronic acid or a salt thereof is 1 to 10% by weight.

4. The hair moisture locking and repair composition according to claim 1, characterized in that the content ratio of the filtrate or solution of the yeast fermentation product to the complex hyaluronic acid or a salt thereof is 1:4 to 9:

1.

5. The hair moisture locking and repair composition according to Claim 1, characterized in that the content ratio of the filtrate or solution of the yeast fermentation product to the complex hyaluronic acid or a salt thereof is 1:3 to 2:

1.

6. The hair moisture locking and repair composition according to Claim 1, characterized in that the mass ratio of oligomeric hyaluronic acid or a salt thereof to low molecular weight hyaluronic acid or a salt thereof is 1:5 to 5:

1.

7. The hair moisture locking and repair composition according to Claim 1, characterized in that the mass ratio of oligomer hyaluronic acid or a salt thereof to low molecular weight hyaluronic acid or a salt thereof is 1:4 to 3:

2.

8. The hair moisture locking and repair composition according to claim 6, characterized in that the molecular weight of the oligomer hyaluronic acid or a salt thereof is 5 kDa or less.

9. The hair moisture locking and repair composition according to claim 6, characterized in that the molecular weight of the low molecular weight hyaluronic acid or a salt thereof is 30 kDa to 50 kDa.

10. The hair moisture locking and repair composition according to claim 6, characterized in that both the salt of the oligomeric hyaluronic acid and the salt of the low molecular weight hyaluronic acid are selected from their sodium salts, potassium salts, calcium salts, zinc salts, or magnesium salts.

11. The hair moisture lock and repair composition according to claim 1, characterized in that the filtrate or solubile of the yeast fermentation product is a fermentation filtrate or solubile obtained by fermenting rice with yeast.

12. The hair moisture locking and repair composition according to claim 1, further characterized in that it contains water, with the remainder being water.

13. Use of the hair moisture locking and repair composition according to claim 1 in a hair product.

14. A hair product characterized in that the functional ingredient contains the hair moisture locking and repair composition described in claim 1.

15. The hair product according to claim 14, characterized in that the content of the hair moisture locking and repairing composition in the hair product is 0.5 to 10%.

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