Method for inducing aneuploidy and / or chromosomal instability in human or non-human primate pluripotent stem cells and diagnostic / therapeutic uses thereof
A novel method induces aneuploidy and chromosomal instability in hPSCs by treating them with chromosome missegregation-inducing compounds, effectively addressing the limitations of current methods and enabling the identification of genes and pathways affected by aneuploidy and the development of therapeutic agents targeting aneuploid cells.
Patent Information
- Application Number
- PCT/US2024/055039
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-05-03
- Filing Date
- 2024-11-08
- Publication Date
- 2025-05-15
AI Technical Summary
Current methods for inducing aneuploidy and chromosomal instability in human pluripotent stem cells (hPSCs) are limited, and there is a need for effective methods to identify genes and pathways affected by different ploidy states and chromosomal stability, as well as to develop therapeutic agents that selectively act on cells with varying ploidy states or chromosomal stability.
A novel method is developed to induce aneuploidy and chromosomal instability in hPSCs by treating euploid cells with compounds that cause chromosome missegregation, such as microtubule inhibitors, resulting in a mixed cell population with a high percentage of aneuploid cells. This method allows for the creation of a durable aneuploid phenotype in hPSCs that maintains normal characteristics, including pluripotency and cell cycle regulation.
The method effectively induces a durable aneuploid state in hPSCs, allowing for the identification of genes and pathways affected by aneuploidy and chromosomal instability, as well as the development of therapeutic agents that selectively target aneuploid cells. The aneuploid hPSCs retain normal characteristics, such as pluripotency and cell cycle regulation, making them suitable for therapeutic and research applications.