Water-soluble decontamination concentrate, decontamination solution, method for producing a decontamination solution, and method for providing a decontamination concentrate

Abstract

The decontamination of surfaces can be made more affordable if the storage life of a decontamination agent used for the decontamination is extended. In particular, this basic concept is based on the finding that, for example, known decontamination solutions discolor or degenerate after some time. The destruction of such allotments ultimately considerably increases the costs of a corresponding decontamination. In particular, a water-soluble decontamination concentrate can comprise a mixture comprising at least three active substances, wherein a first active substance of the three active substances is an organic antioxidant, a second active substance of the three active substances is a metal salt, and a third active substance of the three active substances is at least one surface-active substance. Likewise, a decontamination solution containing at least three active substances, a first of which is an organic antioxidant, a second of which is metal ions, and a third of which is at least one surface-active substance, can be characterized in that the organic antioxidant comprises isoascorbic acid in order to make the decontamination of surfaces more affordable than in the prior art.

Description

[0001] Water-soluble decontamination concentrate, decontamination solution, manufacturing process for manufacturing a decontamination solution and supply process for providing a decontamination concentrate

[0002]

[0001] The invention relates to a water-soluble decontamination concentrate consisting of a mixture comprising at least three active substances, the first of the three active substances being an organic antioxidant, the second of the three active substances being a metal salt, and the third of the three active substances being at least one surfactant. The invention also relates to a decontamination solution consisting of at least three dissolved active substances, the first of the three active substances being an organic antioxidant, the second of the three active substances being at least one metal ion, and the third of the three active substances being at least one surfactant. Furthermore, the invention relates to a manufacturing process for manufacturing a decontamination solution and a supply process for providing a decontamination concentrate.

[0003]

[0002] Decontamination concentrates or decontamination solutions are known, for example, from DE 10 2018 107 149 A1, from DE 28 35 652 A1, from CN 116 731 791 A, from US 9,538,763 B2, from EP 2 489 370 A1, or from EP 2 190 285 B1. However, DE 10 2018 107 149 A1 is aimed at preventing the proliferation of organisms in the water itself when water is left standing for a prolonged period, so that the water solubility of a coating or granulate disclosed therein is not a primary concern. The powder or granules of DE 28 35 652 A1, on the other hand, are used primarily for disinfecting and cleaning contact lenses, which are simply placed in a suitable decontamination solution to clean them. The aim here is to combat aerobic microorganisms without the use of elevated temperatures or the occurrence of allergic reactions, whereby ascorbic acid or glycerol is used.Isoascorbic acid as the enediol, and additionally an oxidizing agent in the form of a water-soluble peroxide and, optionally, a heavy metal salt are to be used. EP 2 190 285 B1 also essentially discusses the antimicrobial use of the decontamination concentrate or the corresponding solution disclosed therein, with the emphasis on the use of biologically active aniline copolymers. The use of a vitamin, metal ions, and a surfactant as a powdered, tabletted, or granulated decontamination concentrate is known, for example, from EP 2489 370 A1. CN 1 16731 ​​791 A also discloses a decontamination solution comprising peroxides and serving essentially for disinfection.

[0004]

[0003] DE 199 36 428 A1 and WO 2006 / 116983 A2 also disclose decontamination solutions intended for the decontaminating surfaces, with particular emphasis on vitamin C or L-ascorbic acid, as well as the use of metal ions. WO 2006 / 116983 A2 synergistically utilizes a surfactant in the decontamination solution to efficiently and simultaneously destroy and remove contaminating proteins, nucleic acids, and microorganisms from surfaces.

[0005]

[0004] The object of the present invention is to make the decontamination of surfaces more affordable than the prior art.

[0006]

[0005] The object of the invention is achieved by water-soluble decontamination concentrates, decontamination solutions, manufacturing processes for manufacturing a decontamination solution, and supply processes for providing a decontamination concentrate having the features of the independent claims. Further advantageous embodiments, possibly also independent of these, can be found in the subclaims and the following description.

[0007]

[0006] The invention is based on the fundamental insight that the decontamination of surfaces can be made more affordable if the storage life of a decontaminating agent used for decontamination is extended. In particular, this basic idea is based on the insight that, for example, the decontamination solution according to WO 2006 / 1 16983 A2 discolors or degenerates after some time. The destruction of such quantities ultimately significantly increases the costs of corresponding decontamination. CN 116 731 791 A or US 9,538,763 B2, which are not generic, also disclose decontamination solutions essentially used for disinfection, which are difficult to store simply due to their volume.

[0008]

[0007] The decontamination of surfaces can be made more affordable than the prior art, regardless of the other combinations of features explained here as being advantageous, by means of a water-soluble decontamination concentrate if the latter comprises a mixture comprising at least three active substances, wherein a first of the three active substances is an organic antioxidant, a second of the three active substances is a metal salt and a third of the three active substances is at least one surface-active substance.Such a design makes it possible to formulate the water-soluble decontamination concentrate in such a way that it is longer-lasting, for example by balancing the pH value or by the presence of suitable preservatives such as alcohol, in particular ethanol, and then to prepare it for the application by adding water or other suitable solvents such that the concentrations or amounts of the three active substances, i.e. the organic antioxidant, the metal salt or the dissolved metal ions and the surface-active substance, are present in the desired amounts or concentrations in the corresponding solution, in order to then be able to carry out decontamination with this solution. In particular, it is possible to prepare only the amount of decontamination solution that is actually required oris required within a reasonable time so that discarding of degenerated decontamination solution can be kept to a minimum.

[0009]

[0008] While the storage life of the water-soluble decontamination concentrate can be significantly increased compared to a corresponding solution with a suitable design, the consumption of the mixture containing the active substances can be optimized with regard to its decontaminating effect by appropriately adjusting the quantities or concentrations in the solution ultimately used for decontamination, so that a minimal amount of this mixture is consumed during decontamination while maintaining the desired decontamination capacity. This also serves to make the decontamination of surfaces as affordable as possible.

[0010]

[0009] Thus, in contrast to the decontamination solutions disclosed in the non-generic CN 116 731 791 A or US 9,538,763 B2, the present decontamination solution preferably consists of at least three dissolved active substances, wherein the first of the three active substances is an organic antioxidant comprising isoascorbic acid, the second of the three active substances is at least one metal ion and the third of the three active substances is at least one surface-active substance.

[0011]

[0010] As already indicated above, the decontamination concentrate itself can be in a fluid form, as long as it remains water-soluble, i.e., for example, hydrophilic. In this case, suitable preservatives, as already described above by way of example, can be added. On the other hand, it may be advantageous to make the water-soluble decontamination concentrate pasty or solid, whereby the mobility, and thus also the reactivity, of the individual components of the decontamination concentrate, i.e., in particular, the mixture comprising the three active substances and the three active substances, is correspondingly limited.

[0011] In particular, a decontamination concentrate can be prepared by blending three active substances, in which a first of the three active substances is an organic antioxidant, a second of the three active substances is a metal salt, and a third of the three active substances is at least one surfactant, into a mixture, and then portioning the mixture in order to make the decontamination of surfaces more affordable than the prior art, regardless of the other combinations of features explained as advantageous here. Depending on the specific implementation, only one surfactant or several different organic antioxidants and / or several metal salts or their complexes or solutions can be added to the mixture.

[0012]

[0012] During addition or mixing, at least one of the three active substances can be added to the mixture in powder form, which, in particular, allows for easy portioning and handling of the corresponding substance. Depending on the specific substance selection, all three active substances, or two or more of the active substances, can be added to the mixture in powder form. The powder form generally allows for good usability and, if the powder is sufficiently fine-grained, has also proven advantageous with regard to sufficient uniformity of the mixture.

[0013]

[0013] On the other hand, it is conceivable that at least one of the three active substances is wetted for mixing. It can therefore be added, for example, in liquid or dissolved form, or in pasty form.

[0014]

[0014] In particular, it is conceivable, for example, that wetting agents, such as water or other aqueous solutions such as citric acid, are added when blending the mixture of the three active substances. This can be done, for example, by directly adding the corresponding liquid or by providing at least one of the substances in pasty form or in dissolved or aqueous form. If necessary, other wetting agents or solvents can also be used accordingly.

[0015]

[0015] During the mixing process, remodeling mechanisms can occur, for example hydration and crystallization of metal ions as metal salts, so that water or other wetting agents or solvents are displaced in such a way that they are no longer freely available and the mixture loses its pasty character or at least partially and thus dries out.

[0016] In this way, hydrating agents or solvents, for example water or other solvents, or even other liquid substances can be added to the mixture, which initially ensure that the mixture acquires a correspondingly fluid or pasty character, which - depending on the specific implementation - can facilitate portioning and further processing, for example pressing.

[0016]

[0017] Depending on the specific design, the wetting agent, for example water or another solvent, can serve to form a complex with one of the three active substances, for example to hydrate, so that after the corresponding reaction, the wetting agent or the solvent, for example the water, is not available for further reactions in the mixture, which can in particular reduce the risk of degeneration of the mixture during storage.

[0017]

[0018] During or after portioning, the mixture can be compressed so that it can be in the form of a pellet or tablet, for example, which can facilitate handling and, in particular, portioning for later use.

[0018]

[0019] A decontamination concentrate in solid form, particularly as a powder, pellet, and / or tablet, is not only relatively easy to handle and portion. It is also conceivable to protect such powders, pellets, or tablets, as well as other solid forms of decontamination concentrate, from degenerating influences in a structurally simple manner, for example, by enclosing them in suitable packaging, such as foils, coatings, blisters, or other tightly sealable containers. When using larger containers, additional measures such as the addition of desiccants and the like can also counteract the generation of degradation.

[0019]

[0020] At least one of the active substances may be present in colloidal form in the decontamination concentrate or in the associated mixtures, which may depend in particular on the type of mixing.

[0020]

[0021] In the present context, colloids are preferably referred to as uniformly distributed aggregations of individual particles or molecular chains that are between 1 nm and up to 1 μm or even larger and evenly distributed. In particular, corresponding metal salts, but also organic antioxidants, can be present in crystalline form, for example in the aforementioned grain size ranges, or in the form of loosely connected molecular groups, for example, molecular groups bound solely by van der Waals forces. Depending on the specific selection of the surface-active substances, this can also apply to the surface-active substances themselves, whereby these may already reach the aforementioned colloidal sizes due to their molecular chain size.

[0021]

[0022] Due to the water solubility of the decontamination concentrate and therefore also of the corresponding mixture comprising the three active substances, a colloidal character, which, for example, during the preparation of the mixture by a mere stirring process, a colloidal formation of one of the three active substances, possibly also two or all of the active substances, can be accepted as long as a solution in water takes place within a reasonable time.

[0022]

[0023] In the present context, the term "mixture" preferably describes any homogeneous or heterogeneous mixture in which at least one component, in particular at least one active substance, is present in solid form, in particular in colloidal form. Depending on the specific preparation of the mixture, the individual components of the mixture may optionally be permeated by air or another gaseous medium in which the mixture was prepared. However, if the mixture is present, for example, in pasty or fluid form, the individual components may also be present in solution or pasty form, in which case components of the wetting agent or a solvent or other liquid may optionally be found between the individual components.

[0023]

[0024] Depending on the specific implementation, at least one disintegrant may be added to the mixture. Such a disintegrant, as well as other additives such as binders, preservatives, etc., can be added to the mixture, particularly prior to portioning, so that they are finely distributed, possibly also colloidally, in the mixture and thus in the decontamination concentrate.

[0024]

[0025] Ultimately, any substance that promotes the dissolution of the decontamination concentrate in water or another hydrophilic solvent can serve as a disintegrant. In particular, a disintegrant can be used as a disintegrant. Any substance that expands significantly upon contact with water or another hydrophilic solvent, for example, transforms into a gaseous state due to a chemical reaction, can serve as a disintegrant or even as a disintegrant.

[0026] Inert substances can be used as disintegrants or explosives, whereby in this context, "inert" refers in particular to substances that do not actively participate in the actual decontamination.

[0025]

[0027] Depending on the specific implementation, the disintegrant can provide a basic contribution, thus bringing the pH value of a solution prepared from the decontamination concentrate to a desired level. Depending on the specific implementation, the corresponding basic agent used as a disintegrant may not participate in the actual decontamination, i.e., be inert. On the other hand, it is also conceivable that this basic agent also has a decontaminating effect and is used accordingly when rubbed and / or sprayed for decontamination.

[0026]

[0028] Sodium bicarbonate (sodium bicarbonate NaHCO3), sodium citrate (C6H5Na3O7) and / or sodium sulfate (NaiSO+) have proven particularly advantageous as disintegrants.

[0027]

[0029] As already explained above, an appropriate decontamination solution can be provided by dissolving the decontamination concentrate.

[0028]

[0030] In particular, a predetermined amount of the decontamination concentrate can be dissolved in a predetermined volume of a predetermined solvent. In this way, it can be ensured with relative reliability that an appropriate amount of the decontamination concentrate or a desired concentration of the active substances is present in the corresponding solution for the desired decontamination effect. This, in turn, ensures the most affordable possible design for the decontamination of surfaces compared to the state of the art, since ultimately only the necessary amount of active substances or decontamination concentrate is provided in the solution.

[0029]

[0031] In particular, the quantities can be specified in such a way that the specified amount is to be dissolved in 100 ml or 1 l of the specified solvent. This ensures, on the one hand, that a sufficient amount of decontamination solution is available for surface treatment by rubbing or spraying, but on the other hand, the amount of decontamination solution provided is not so high that it has to be stored for a long time until it is used up. On the other hand, the loss of such small amounts of decontamination solution can certainly be accepted if this appears economically sensible. In practice, however, it can be assumed that this will generally not be the case with such a small supply of decontamination solution.It is clear that, if desired, even larger quantities of decontamination solution can be provided quickly and reliably by increasing the amount of decontamination concentrate in a correspondingly larger volume of solvent. For example, double the amount can be dissolved in 2 liters of solvent if a corresponding amount of decontamination solution will be used quickly.

[0030]

[0032] By stocking a decontamination concentrate, which inherently has a longer shelf life, the economic risk associated with surface decontamination can be effectively minimized. Shipping and distribution of decontamination concentrate is also cost-effective, allowing it to be used effectively and cost-effectively, especially in more distant countries where clean working practices are just as essential as in Western countries. This allows for the cost-effective maintenance of appropriate pharmaceuticals and laboratory equipment on-site.

[0031]

[0033] Water is a particularly suitable solvent, as it is generally readily available at low cost, especially more cost-effectively than other solvents. If necessary, an alcohol or an aqueous mixture of alcohols can also be used. Ethanol, for example, absolute ethanol, optionally in a corresponding aqueous solution, denatured or undenatured, has proven particularly advantageous as an alcohol.

[0032]

[0034] The organic antioxidant may, in particular, comprise isoascorbic acid. This includes, in particular, its derivatives and salts, since salts, in particular, can be easily blended into a corresponding mixture and, if appropriate, the corresponding salts can provide metal ions, which can be used in the solution as a complementary decontaminating agent.

[0033]

[0035] In the present context, derivatives are in particular substances which, in relation to the parent compound, for example isoascorbic acid, have a structural unit which is similar to the functional group of the parent compound and contains a structural element of this functional group in the same oxidation state.

[0034]

[0036] In this context, isoascorbic acid, also called erythorbic acid or arboascorbic acid, is used, particularly its D-enantiomer, isoascorbic acid. On the other hand, the L-enantiomer is also conceivable, or mixtures of the two enantiomers. In contrast to the prior art, which describes vitamin C in particular as a beneficial organic antioxidant, isoascorbic acid proves to be more stable and therefore more cost-effective. In particular, isoascorbic acid can be produced relatively inexpensively from sucrose, without, for example, the need to use natural reserves of vitamin C for decontamination.

[0035]

[0037] On the other hand, isoascorbic acid is known from food technology, so that its use as a decontamination agent ultimately appears justifiable in view of any physical impairment or danger to personnel or persons who carry out the decontamination or provide the decontamination solution.

[0036]

[0038] In contrast to the state of the art, a substance which does not necessarily have to be separately introduced into the animal body for metabolism appears to be advantageously usable as an active substance for the decontamination, in particular of surfaces of proteins, nucleic acids, DNA, RNA or nucleases or microorganisms, so that isoascorbic acid in particular can also make a synergistic contribution to the decontamination process, which ultimately enables effective decontamination.

[0037]

[0039] In this respect, isoascorbic acid enables correspondingly effective contamination due to its synergistic interaction with the other active substances and also enables increased storage capacity due to its relatively high inherent stability.

[0038]

[0040] Accordingly, a decontamination solution containing at least three active substances, wherein a first of the three active substances is an organic antioxidant, a second of the three active substances is metal ions, and a third of the three active substances is at least one surfactant, can be characterized in that the organic antioxidant comprises isoascorbic acid in order to make the decontamination of surfaces more affordable than the prior art. The latter appears to be made possible by an increased storage life of the decontamination solution comprising isoascorbic acid.

[0039]

[0041] The metal ions may include, in particular, copper and / or zinc ions. These

[0040] Ions have proven particularly effective in decontamination when used synergistically with isoascorbic acid. It is understood that, depending on the specific implementation, other metal ions, such as silver, barium, or calcium ions, may also be used. In particular, corresponding ionic complexes or, if appropriate, ionic molecules may also be used if they are present as corresponding salts.

[0042] Accordingly, it may be advantageous to use metal salts such as copper sulfate, especially copper sulfate pentahydrate (QiSO / äfFO) (also called copper vitriol or blue galician stone or bluestone), or zinc chloride (ZnCl?) or other corresponding metal salts.

[0041]

[0043] The metal ions or metal salts can be used to provide the second of the three active substances, in particular. To the extent that isoascorbic acid can possibly be provided in the form of salts, it is conceivable that if these salts comprise, for example, copper ions, zinc ions, silver ions, or similar ions, both the first and second of the three active substances in the decontamination concentrate are provided by the corresponding salts.

[0042]

[0044] In particular, the metal ions can include divalent and / or trivalent ions of metals of Period 4 and / or transition groups I, II, and VIII of the Periodic Table of the Elements. They are preferably used in the form of their salts with organic and / or inorganic acids or bases, which can optionally be used to balance the pH.

[0043]

[0045] In this context, a surface-active substance is understood to mean, in particular, any substance soluble in water or present in the decontamination solution that promotes the contact and reactivity of the decontamination solution. Preferably, the surface-active substance is inert, so that it does not actively participate in the decontamination process, i.e., it cannot solely serve to denature or decompose and remove surface-contaminating proteins, nucleic acids, DNA, RNA, or nucleases, especially ribonucleases, and microorganisms.

[0044]

[0046] Preferably, any form of wetting agent is suitable as a surface-active substance. In this context, any substance that reduces the surface tension of the decontamination solution or of a decontamination solution provided by the decontamination concentrate can be used as a wetting agent. An emulsifier can be used as a surface-active substance, in particular, in addition to or as an alternative. It is also conceivable that a wetting agent could also be used as an emulsifier.

[0045]

[0047] In particular, non-ionic surfactants or anionic surfactants can be used as surface-active substances or as wetting agents or emulsifiers.

[0048] For example, Tween 20 (polyoxyethylene(20) sorbitan monolaurate or poly(oxy-l,2-ethanediyl) monododecanoic acid sorbityl ester), also called polysorbate 20, or SDS (sodium dodecyl sulfate) or SLS (sodium lauryl sulfate) can be used as a surface-active substance.

[0046]

[0049] Anionic, non-ionic, amphoteric, or cationic surfactants, especially if they are inert, or suitable mixtures thereof, can be used as surface-active substances. In particular, alkyl ether sulfates, alkyl and / or aryl sulfonates, alkyl sulfates, amphoteric surfactants, betaines, alkylamidoalkylamines, acylated amino acids, alkyl-substituted amino acids, alkyl-substituted imino acids, articulated amino acids, amphoteric surfactant combinations, and the like can be used. In particular, all inert surfactants appear suitable for synergistically positively influencing the decontamination process.

[0047]

[0050] The isoascorbic acid can preferably be present in the decontamination solution in an amount between 0.001 mol / l and 1 mol / l. Depending on the specific situation, a lower limit of 0.002 mol / l or 0.005 mol / l can be particularly advantageous. It is understood that the respective lower limit can ensure a sufficient contribution of isoascorbic acid to the decontamination process, regardless of the upper limit. In particular, the upper limit of isoascorbic acid can be 0.9 mol / l or 0.7 mol / l to avoid the unnecessary use of isoascorbic acid. Depending on the specific implementation, 0.01 mol / l or 0.1 mol / l of isoascorbic acid can be particularly advantageous as an upper limit or lower limit.

[0048]

[0051] Accordingly, it may be advantageous if more than 30 wt.%, in particular more than 40 wt.%, of the active substances in the decontamination concentrate are organic antioxidants, for example isoascorbic acid. In particular, more than 90 mol.%, in particular more than 83 mol.%, of the active substances in the decontamination concentrate can be organic antioxidants, for example isoascorbic acid. Cumulatively or alternatively, 80 wt.%, in particular 75 wt.%, or less of the active substances in the decontamination concentrate can be organic antioxidants, so that weight fractions still remain for the remaining active substances and other additives. The latter also applies if no more than 54 mol.%, in particular no more than 40 mol.%, of the active substances in the decontamination concentrate are organic antioxidants.

[0052] In particular, the metal ions in the decontamination solution can be present in an amount of more than 0.0001 mol / l. In particular, this can be more than 0.0003 mol / l or 0.0004 mol / l, so that a sufficient concentration or amount of metal ions can be ensured. Preferably, the amount or concentration of metal ions in the decontamination solution does not exceed 0.1 mol / l, in particular not exceeding 0.9 mol / l or 0.8 mol / l. In particular, no less than 0.0005 mol / l and / or no more than 0.001 mol / l of metal ions can be used in this regard.

[0049]

[0053] In this respect, not less than 5 wt.%, in particular not less than 8 wt.%, of the active substances in the decontamination concentrate can be metal salts in order to ensure a sufficient amount of metal ions in the decontamination solution. This also applies in particular if not less than 4 mol.%, in particular not less than 6 mol.%, of the active substances in the decontamination concentrate are metal salts. To ensure that sufficient weight fractions remain for the other active substances, it is advantageous if not more than 20 wt.%, in particular not more than 15 wt.%, or not more than 25 mol.%, in particular not more than 15 mol.%, of the active substances in the decontamination concentrate are metal salts.

[0050]

[0054] Preferably, the surface-active substances are present in the decontamination solution in an amount of at least 0.001 mol / l, in particular 0.002 mol / l, or at least 0.1 g / l, in particular 0.2 g / l, so that a sufficient synergistic contribution to decontamination can be ensured. In particular, it appears cumulatively or alternatively advantageous if the surface-active substances are present in the decontamination solution in an amount of 1 mol / l, in particular 0.9 mol / l, or 100 g / l, in particular 90 g / l, or less, in order to avoid unnecessary use of the substance.

[0051]

[0055] Accordingly, it appears advantageous if not less than 10 wt.%, in particular not less than 17 wt.%, or not less than 5 mol.%, in particular not less than 11 mol.%, of the active substances in the decontamination concentrate are surface-active substances in order to be able to provide a sufficient amount of surface-active substance for operational reliability. To ensure that sufficient weight fractions remain for the other components and, in particular, to avoid using an unnecessarily large amount of material, it appears advantageous if not more than 60 wt.%, in particular not more than 46 wt.%, or not more than 40 mol.%, in particular not more than 32 mol.%, of the active substances are surface-active substances.

[0056] The decontamination concentrates and decontamination solutions described here as advantageous are particularly suitable for the efficient and simultaneous destruction and removal of contaminating proteins, nucleic acids, DNA, RNA, or nucleases, especially ribonucleases, and microorganisms from surfaces such as laboratory benches, corridors, equipment, and instruments.

[0052]

[0057] With a suitable design, aggressive chemicals or highly oxidizing reagents can be dispensed with. These decontamination concentrates or decontamination solutions are particularly suitable for the renaturation, modification, degradation, solubilization, and removal of proteins, nucleic acid molecules, and microorganisms, especially DNA, RNA, and nucleases such as RNases or DNases, from all types of surfaces. With a suitable design, the surfaces are not attacked or are attacked only minimally. Any adverse effects on the user, such as irritation of the skin, mucous membranes, or the respiratory tract, can also be minimized.

[0053]

[0058] Decontamination can usually be achieved by spraying and / or rubbing the appropriate decontamination solution onto the surfaces to be treated. The contact time can be adjusted to suit the specific requirements and is typically between 30 seconds and 2 minutes, depending on the specific temperature and composition of the decontamination solution.

[0054]

[0059] The need for appropriate decontamination solutions proves to be particularly helpful in light of modern pharmaceuticals and laboratory medicine, which increasingly rely on protein and nucleic acids and their potentiation, since in corresponding processes foreign proteins or nucleic acids or fragments thereof are also amplified or enriched accordingly and falsify measurement results or the results of the desired reaction processes.

[0055]

[0060] It is understood that the features of the solutions described above or in the claims may also be combined if necessary in order to be able to implement the advantages cumulatively.

[0056]

[0061] Further advantages, objectives, and properties of the present invention will be explained with reference to the following description of exemplary embodiments, which are also particularly illustrated in the accompanying drawings. In the drawings, the single table shows various decontamination concentrates and associated decontamination solutions; and the single figure shows a gel pattern after DNA degradation with various decontamination and comparison solutions.

[0057]

[0062] The table shows six decontamination concentrates as examples, which differ mainly in their content of isoascorbic acid.

[0058]

[0063] Furthermore, the first three decontamination concentrates contain only SDS as the surfactant, while the second three decontamination concentrates contain Tween 20 as the surfactant in addition to SDS. It is understood that further combinations are readily conceivable and feasible in different embodiments.

[0059]

[0064] All decontamination concentrates also contain copper(II) sulfate pentahydrate and zinc chloride, although in different embodiments, other metal salts may be used as suppliers of metal ions or other metal ions.

[0060]

[0065] The first three decontamination concentrates, which contain only SDS as a surfactant, contain citric acid to adjust the pH value or for stabilization. This acid can be incorporated into the decontamination concentrate if necessary and serves to hydrate or bond the remaining colloidal components. Alternatively, it can also be added to a solution of the corresponding decontamination concentrates. The latter also applies to ethanol, which serves to stabilize the second three decontamination concentrates and, depending on the specific application, can be incorporated into the respective decontamination concentrate or, in the specified quantities, for example, 50 ml, can serve as a solvent.

[0061]

[0066] The corresponding decontamination concentrates were then dissolved in 1 liter of a decontamination solution and proved to be sufficiently stable and effective in decontaminating. In particular, it was noted that even mixtures of these decontamination concentrates in solid or pasty form could be stored with sufficient stability.

[0062]

[0067] The table shows the weight and mole numbers in 1 l of the corresponding decontamination solution as well as the weight percent (wt%, wt%) or mole percent (mol%) of the antioxidants, metal salts and surface-active substances used in relation to the total of these active substances.

[0068] Blank samples AS4 were provided from the second group of decontamination concentrates and solutions. Samples AS1 were provided in corresponding blank samples containing the same amount of ascorbic acid instead of isoascorbic acid. Corresponding samples AS2 containing ascorbyl glycolate and AS3 containing sodium isoascorbate (E 316; CAS Reg. No. 6381-77-7) were also provided.

[0063]

[0069] For testing, aliquots of lambda DNA (45 kb), 200 ng for each sample, were mixed with 10 μl of nuclease-free water and then added with 5 μl of the respective decontamination solution. Each sample was incubated for 3 minutes and 10 minutes at 20°C. The samples were then mixed with 3 μl of loading powder and denatured for 3 minutes at 92°C. The mixture was then loaded onto a 1% agarose gel and run for 60 minutes.

[0064]

[0070] Figure 1 shows the corresponding gel image taken with a mobile phone camera after the gel had run for 60 minutes.

[0065]

[0071] In addition and for comparison, figure one shows a marker M 100 bp, which represents a synthetic DNA marker with bands 100 to 1 kbp in hundredths and additionally 1.5 kbp and 3.0 kbp.

[0066]

[0072] There is also a control C with lambda DNA and nuclear-free water instead of the decontamination solutions in Figure 1.

[0067]

[0073] Likewise, the designation Asc low or Asc high refers to a decontamination solution containing ascorbic acid instead of isoascorbic acid as an antioxidant in order to objectively determine the quality of the experimenter and his record writer, which was known to the experimenter and his record writer and which, however, corresponded to the corresponding samples of group AS 1.

[0068]

[0074] Furthermore, the measurements were conducted in a blind study for the experimenter and their record-keeper. The participants were therefore unaware of which substances were contained in the designations ADI to AD4.

[0069]

[0075] It is evident that substance AD2 did not appear suitable for decontamination. In contrast, decontamination solution AD4 delivered comparable values ​​to decontamination solution ADI in terms of its decontamination readiness. Regarding decontamination solution AD3, satisfactory results were only obtained at higher concentrations of sodium isoascorbate, although it is assumed that better results can be readily achieved by appropriately selecting the pH value and exposure times. However, even with decontamination solution AD3, significant reductions compared to control C were achieved even at low concentrations.

Claims

Patent claims:

1. Water-soluble decontamination concentrate consisting of a mixture comprising at least three active substances, wherein the first of the three active substances is an organic antioxidant, the second of the three active substances is a metal salt and the third of the three active substances is at least one surface-active substance, characterized in that the organic antioxidant comprises isoascorbic acid and / or the decontamination concentrate is solid.

2. Decontamination concentrate according to claim 1, characterized in that the decontamination concentrate is in the form of a powder, a pellet and / or a tablet.

3. Decontamination concentrate according to claim 1 or 2, characterized in that the at least one organic antioxidant, the at least one metal salt and / or the at least one surface-active substance are present in colloidal form.

4. Decontamination concentrate according to one of claims 1 to 3, characterized in that between 30% by weight and 80% by weight of the active substances are organic Antioxidant, between 5% and 20% by weight of the active ingredients are metal salt and between 10% and 60% by weight of the active ingredients are surface-active substances.

5. Decontamination concentrate according to one of claims 1 to 4, characterized in that between 90 mol% and 54 mol% of the active substances are organic antioxidant, between 4 mol% and 25 mol% of the active substances are metal salt and between 5 mol% and 40 mol% of the active substances are surface-active substance.

6. Decontamination concentrate according to one of claims 1 to 5, characterized in that the metal salt comprises copper sulfate pentahydrate and / or zinc chloride.

7. Decontamination concentrate according to one of claims 1 to 6, characterized in that at least one disintegrant is added to the mixture.

8. Decontamination concentrate according to one of claims 1 to 7, characterized in that the surface-active substance comprises a non-ionic surfactant and / or an anionic surfactant.

9. A manufacturing process for producing a decontamination solution, characterized in that a predetermined amount of the decontamination concentrate according to one of claims 1 to 8 is dissolved in a predetermined volume of a predetermined solvent.

10. Manufacturing method according to claim 9, characterized in that the predetermined amount of the decontamination concentrate is dissolved in 100 ml or 1 1 and / or that the predetermined amount of the decontamination concentrate is dissolved in a volume of water, an alcohol or a mixture of these 11. Decontamination solution consisting of at least three dissolved active substances, wherein the first of the three active substances is an organic antioxidant, the second of the three active substances is at least one metal ion and the third of the three active substances is at least one surface-active substance, characterized in that the organic Antioxidant isoascorbic acid.

12. Decontamination solution according to claim 1 1, characterized in that the decontamination solution is an aqueous solution and / or is alcohol-based.

13. Decontamination solution according to claim 11 or 12, characterized in that the isoascorbic acid is present in an amount between 0.001 mol / l and 1 mol / l, the metal ions in an amount between 0.0001 mol / l and 0.1 mol / l and the surface-active substances in an amount between 0.001 mol / l and 1 mol / l or between 0.1 g / l and 100 g / l.

14. Decontamination solution according to claim 11 to 13, characterized in that the metal ions comprise copper and / or zinc ions.

15. Decontamination solution according to one of claims 11 to 14, characterized in that the surface-active substance comprises a non-ionic surfactant and / or an anionic surfactant.

16. A method for providing a decontamination concentrate according to any one of claims 1 to 8, characterized in that three active substances, wherein a first of the three active substances is an organic antioxidant, a second of the three active substances is a metal salt and a third of the three active substances is at least one surface-active substance, are mixed together to form a mixture and the mixture is then portioned.

17. Preparation method according to claim 16, characterized in that additives are also added to the mixture before portioning.

18. A method of preparation according to claim 16 or 17, characterized in that at least one of the three active substances is added to the mixture in powder form.

19. A method of providing according to any one of claims 16 to 18, characterized in that at least one of the three active substances is wetted for mixing.

20. Preparation method according to one of claims 16 to 19, characterized in that the mixture is compressed during or after portioning.

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