Method for automated preparation of a regenerative epidermal suspension

Abstract

Abstract A method for automated preparation of a regenerative epidermal suspension in a cartridge wherein the cartridge comprises a cover, a cup containing a tissue sample, and a well plate situated beneath the cover, the well plate comprising a first well containing a quantity of enzyme solution and a second well containing a quantity of buffer solution. The method comprising receiving, by a processor, an initiation signal, via the processor, executing a sequence, wherein the sequence comprises actuating a tissue disaggregator against the tissue sample in the presence of the enzyme solution with the cup positioned in the first well, raising the cup to an upper position, rotating the well plate to position the second well beneath the cup, lowering the cup to a lower position within the second well, and actuating the tissue disaggregator against the tissue sample in the presence of the buffer solution with the cup positioned in the second well. Abstract A method for automated preparation of a regenerative epidermal suspension in a cartridge wherein the cartridge comprises a cover, a cup containing a tissue sample, and a well plate situated beneath the cover, the well plate comprising a first well containing a quantity of enzyme solution and a second well containing a quantity of buffer solution. The method comprising receiving, by a processor, an initiation signal, via the processor, executing a sequence, wherein the sequence comprises actuating a tissue disaggregator against the tissue sample in the presence of the enzyme solution with the cup positioned in the first well, raising the cup to an upper position, rotating the well plate to position the second well beneath the cup, lowering the cup to a lower position within the second well, and actuating the tissue disaggregator against the tissue sample in the presence of the buffer solution with the cup positioned in the second well. PCT / US2023 / 085844wo 2024 / 145258 1 / 51 101 FIG. 1 20 25 20 84 78 24 J ul 2 02 5 P C T / U S 2 0 2 3 / 0 8 5 8 4 4 w o 2 0 2 4 / 1 4 5 2 5 8 1 / 5 1 1 0 1 F I G . 1 P C T / U S 2 0 2 3 / 0 8 5 8 4 4 w o 2 0 2 4 / 1 4 5 2 5 8 1 0 1 F I G . 1

Claims

1. A method for automated preparation of a regenerative epidermal suspension in a cartridge wherein the cartridge comprises:a cover,a cup containing a tissue sample, anda well plate situated beneath the cover, the well plate comprising a first well containing a quantity of enzyme solution and a second well containing a quantity of buffer solution;the method comprising:receiving, by a processor, an initiation signal,via the processor, executing a sequence, wherein the sequence comprises: actuating a tissue disaggregator against the tissue sample in the presence of the enzyme solution with the cup positioned in the first well;raising the cup to an upper position;rotating the well plate to position the second well beneath the cup;lowering the cup to a lower position within the second well; andactuating the tissue disaggregator against the tissue sample in the presence of the buffer solution with the cup positioned in the second well.

2. The method of claim 1, wherein the initiation signal indicates that a cartridge has been placed on a sensor.

3. The method of claim 1, wherein the tissue disaggregator exerts a rotational or grinding force on the tissue sample.

4. The method of claim 1, wherein the tissue disaggregator comprises a pestle.

5. The method of claim 1, wherein the tissue disaggregator comprises a raised surface element.

6. The method of claim 1, wherein the tissue disaggregator comprises a plurality of pips.

7. The method of claim 1, wherein the tissue sample is disposed along a screen disposed within the cup.

8. The method of claim 7, wherein the screen is positioned transversely within the cup.9.The method of claim 7, wherein the screen is configured to separate outparticulates above 100 microns in size.

10. The method of claim 7, wherein the screen is configured to separate out particulates above 50 microns in size.

11. The method of claim 7, wherein the screen is configured to separate out particulates above 10 microns in size.

12. The method of claim 1, further comprising activating a housing motor, the housing motor configured to operate the tissue disaggregator.

13. The method of claim 12, wherein the housing motor is configured to raise and lower the cup.

14. The method of claim 1, further comprising pressing the tissue disaggregator against the tissue sample a plurality of times when the cup is positioned in the first well and the second well.

15. The method of claim 1, further comprising actuating the tissue disaggregator against the tissue sample when the cup is in the upper position.

16. The method of claim 1, further comprising activating a base plate motor, the base plate motor configured to rotate the well plate.

17. The method of claim 1, further comprising rotating the well plate to position the cup in fluidic contact with a second quantity of buffer solution.

18. The method of claim 17, wherein the second quantity of buffer solution is contained within a third well of the well plate.

19. The method of claim 18, further comprising actuating the tissue disaggregator against the tissue sample in the presence of the second quantity of buffer solution with the cup positioned in the third well, wherein the third well comprising one or more raised processing elements.

20. The method of claim 1, further comprising receiving a completion signal indicating that a regenerative epidermal suspension is present.

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