Improved method for automated preparation of a regenerative epidermal suspension

Abstract

Abstract A method for automated preparation of a regenerative epidermal suspension is disclosed. A cartridge containing a tissue sample, a cup and a well plate having wells containing enzyme solution and buffer solution is positioned within a processing system. In response to an initiation signal, a processor controls operation of a tissue disaggregator to mechanically disaggregate the tissue sample while sequentially exposing the tissue sample to the enzyme solution and the buffer solution. The tissue disaggregator may apply rotational, grinding and / or pressing forces to the tissue sample supported on a screen within the cup. The processor may further control movement of the cup, rotation of the well plate and operation of one or more motors to automate preparation of the regenerative epidermal suspension. Abstract 20 26 20 46 06 1 6 Ju n 20 26 wo 2024 / 145258 PCT / US2023 / 085844 16 Jun 2026 303 301 * 2026204606 300 308 305 00 307 306 309 312 310 314 311 315 313 316 317 321 317a 318 320 319 322 FIG. 3A

Claims

2026204606   16 Jun 20261. A method for automated preparation of a regenerative epidermal suspension in a cartridge wherein the cartridge comprises:a cover,a cup containing a tissue sample, anda well plate situated beneath the cover;the method comprising:receiving, by a processor, an initiation signal,via the processor, executing a sequence, wherein the sequence comprises:actuating a tissue disaggregator against the tissue sample in the presence of the enzyme solution with the cup positioned in the well;andactuating the tissue disaggregator against the tissue sample in the presence of the buffer solution with the cup positioned in the well.

2. The method of claim 1, wherein the initiation signal indicates that a cartridge has been placed on a sensor.

3. The method of claim 1, wherein the tissue disaggregator exerts a rotational or grinding force on the tissue sample.

4. The method of claim 1, wherein the tissue disaggregator comprises a pestle.

5. The method of claim 1, wherein the tissue disaggregator comprises a raised surface element.

6. The method of claim 1, wherein the tissue disaggregator comprises a plurality of pips.

7. The method of claim 1, wherein the tissue sample is disposed along a screen disposed within the cup.

8. The method of claim 7, wherein the screen is positioned transversely within the cup.

9. The method of claim 7, wherein the screen is configured to separate out particulates above 100 microns in size.

10. The method of claim 7, wherein the screen is configured to separate out particulates above 50 microns in size.2026204606   16 Jun 202611. The method of claim 7, wherein the screen is configured to separate out particulates above 10 microns in size.

12. The method of claim 1, further comprising activating a housing motor, the housing motor configured to operate the tissue disaggregator.

13. The method of claim 12, wherein the housing motor is configured to raise and lower the cup.

14. The method of claim 1, further comprising pressing the tissue disaggregator against the tissue sample a plurality of times when the cup is positioned in the well.

15. The method of claim 1, further comprising actuating the tissue disaggregator against the tissue sample when the cup is in an upper position.

16. The method of claim 1, further comprising activating a base plate motor, the base plate motor configured to rotate the well plate.

17. The method of claim 1, further comprising receiving a completion signal indicating that a regenerative epidermal suspension is present.

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