DEVICE FOR TISSUE STIMULATION WITH ELECTROMAGNETIC FIELDS

Abstract

This disclosure relates to methods and devices for tissue stimulation with electromagnetic fields by means of frequency sweeping. This frequency sweeping refers to the variation of the electromagnetic field applied to a tissue through increments in frequency deltas, which adapts to the tissue's impedance response. Furthermore, some methods in this disclosure allow the determination of stimulation frequency bands in order to focus stimuli on these bands until the tissue's impedance response returns to a tolerance level or exceeds a maximum stimulation time.Devices for stimulating tissue through electromagnetic fields consist of a computing unit; a decoupling circuit connected to an external power source and the computing unit; an array of electromagnetic transducers connected to the computing unit and the decoupling circuit; the computing unit executes the disclosed methods to generate activation signals that are received by the array of electromagnetic transducers.

Description

1 / 58 DEVICE FOR TISSUE STIMULATION WITH ELECTROMAGNETIC FIELDS CROSS-REFERENCES FOR RELATED APPLICATIONS

[001] This application claims the priority benefit of registration in Colombian patent application number NC2018 / 0001283, registered on 07 / 02 / 2018. The entirety of the aforementioned patent application is incorporated herein by reference and forms part of this application.

[002] The tissue stimulation disclosed in this document is related to registration in Colombia with serial number NC2018 / 0001283, registered on 07 / 02 / 2018. DESCRIPTION OF THE PREVIOUS TECHNIQUE Technical Field

[003] This disclosure relates to methods of tissue stimulation with electric, magnetic and electromagnetic fields through frequency sweeping, which refers to the variation of electric, electromagnetic or magnetic vector fields through increments of frequency deltas from an initial stimulation frequency to a final stimulation frequency. These stimulation methods have applications in identifying tissue anomalies and potentially correcting these anomalies, including, for example, identifying cancerous tissues and reversing the growth and proliferation of this type of tissue. State of the Art

[004] It is well known that changes in a cell's electrical environment can affect normal cell homeostasis. More specifically, cell homeostasis is related to the maintenance of a healthy membrane potential, and changes in membrane potential have connections to uncontrolled cell proliferation and differentiation (metastasis). As reported by Yang and Brackenbury (2013):

[005] “The membrane potential (Vm), the voltage across the plasma Petition 870260051474, dated 05 / 28 / 2026, page 16 / 94 2 / 58 of the membrane, arises from the presence of different ion channels or transporters, with specific ionic selectivity and permeability. Vm is a key biophysical signal in non-excitable cells, modulating important cellular activities such as proliferation and differentiation. Therefore, the multiplicities of different ion channels or transporters expressed on different cells are fine-tuned so that Vm is regulated. It is well established that cancer cells have different bioelectrical properties. Notably, electrophysiological analyses in various types of cancer cells have revealed a depolarized Vm that favors cell proliferation. Ion channels or transporters control cell volume and migration, and recent data suggest that the level of Vm has a functional role in the cell migration of cancer cells. ...Data showing that Vm fluctuation can functionally regulate tumorigenesis and differentiation, and even promote cancer progression, may be useful as a potential marker for tumor detection and treatment, with prognostic value.”

[006] In addition to simply detecting tissue abnormalities, the literature has also suggested that affecting the cellular environment to produce cell membrane hyperpolarization can reverse tumor development and metastasis (see e.g., Ingber, Can cancer be reversed by engineering the tumor microenvironment?, Semin Cancer Biol. 2008 Oct; 18(5): 356-364; Lobikin, Chernet, Lobo and Levin, Resting Potential, Oncogene-induced Tumorigenesis, and Metastasis: The Bioelectric Basis of Cancer in vivo, Phys Biol. 2012 Dec; 9(6): 065002; 2018). Lobikin et al.A mechanistic dissection of the pathways by which the host restarts cancer cells may give rise to strategies that normalize cancer [citations removed], in. Petition 870260051474, dated 05 / 28 / 2026, page 17 / 94 3 / 58 In contrast to current approaches that seek to kill tumors and thus risk a compensatory proliferation response by some remaining cancerous cell. ... More attractively, forced hyperpolarization ... through molecular-genetic or pharmacological means can functionally reduce the incidence of tumors. ... It is hoped that, by unraveling the fundamental roles of bioelectricity in pattern formation, biomedicine may one day activate the remarkable pathways that highly regenerative model species use to normalize and not kill tumor tissue....

[007] The previous state of the art, as well as the articles cited above, is full of suggestions for the use of pharmaceutical and molecular biology tools targeting ion channels in the regulation of membrane potential and obtaining the anticancer results suggested earlier. However, the proposed solutions for applying electric fields in the identification of cancerous tissues, or in reversing tumor formation, are very limited. Kadir et al. (2018) mention, “even in the late 1930s, tumors were detected based on their voltmeter readings (Burr et al., 1938; Burr, 1940).” More recently, the application of electric fields in cancer treatment has been proposed and implemented, for example, by Palti in US Patent US7333852B2 (designated as tumor treatment fields or TTFs).The clinical results of these TTFs are described in the publication, Kirson, Dbaly, Tovarys, Vymazal, Soustiel, Itzhaki, Mordechovich, SteinbergShapira, Gurvich, Schneiderman, Wasserman, Salzberg, Ryffel, Goldsher, Dekel and Palti, Alternating electric fields arrest cell proliferation in animal tumor models and human brain tumors, PNAS June 12, 2007 104 (24) 1015210157. The inhibitory effect of TTFs on tumors has been attributed mainly to two separate mechanisms: interference with mitotic spindle microtubule formation and physical destruction. Petition 870260051474, dated 05 / 28 / 2026, p. 18 / 94 4 / 58 of the cells during cleavage, both of which are strongly dependent on the orientation of the mitotic axis versus the field vectors. According to information provided by Novocure (see, https: / / biopharmadealmakers.nature.com / users / 38001-novocure / posts / 16531ttfields-a-radical-new-approach-to-cancer-treatment-using-electric-fields), a company that markets a TTF solution under the OPTUNE brand and is the signatory of the aforementioned '852 patent, “the inhibition of cell proliferation and accelerated cell death by TTFs is optimal at intensities of 1-5 V cm-1 and frequencies between 100 and 500 kHz, depending on the cell type – the mitotic spindle is best interrupted at 150 kHz in the case of pancreatic cancer and NSCLC, and at 200 kHz in ovarian cancer and GBM. Mitosis in non-cancerous cells is typically interrupted at frequencies around 50 kHz.” For optimal results, Novocure adds that TTFs should be applied for at least 18 hours a day.In this sense, the average daily usage time of this device (i.e., the time spent undergoing treatment) is a crucial component of the clinical benefit. SUMMARY

[008] This disclosure relates to methods and devices for stimulating tissues with electromagnetic fields by frequency sweeping, and more specifically, to a first method of stimulating tissues from an electric field by frequency variation, a second method for stimulating tissues with a magnetic field by frequency variation, a third method that combines tissue stimulation with electric and magnetic fields by frequency variation, and a device for stimulating tissues with electromagnetic fields.

[009] The first method of tissue stimulation with electromagnetic fields comprises the following steps: a) application of an electric field stimulation to a tissue through an array of electromagnetic transducers that receives an activation signal, whose Petition 870260051474, dated 05 / 28 / 2026, page 19 / 94 5 / 58 frequency varies from an initial tissue stimulation frequency (fie) to a final tissue stimulation frequency (ffe), with increments or decrements in steps of the frequency delta (Δfe) during a time delta (Δte); b) measurement of the tissue impedance response to the stimulus in step (a); c) establishment of a reference level with the tissue impedance response measured in step (b); d) establishment of a tolerance (NT) for the reference level established in step (c); e) determination of lower tissue stimulation frequencies (fbx) as the point where the tissue impedance response falls below the tolerance (NT) established in step (d); f) determination of higher tissue stimulation frequencies (ftx) as the point where the tissue impedance response returns to the tolerance (NT) established in step (d);where the higher tissue stimulation frequencies (ftx) are greater than the lower tissue stimulation frequencies (fbx), ex is a natural number greater than or equal to 1.

[0010] The second method of tissue stimulation with electric fields comprises the following steps: a') application of a magnetic field stimulus to a tissue through an array of electromagnetic transducers that receive an activation signal, whose frequency varies from an initial tissue stimulation frequency (fm) to a final tissue stimulation frequency (ffm), with increments or decrements in steps of the frequency delta (Δfm) during a time delta (Δtm); b') measurement of the tissue impedance response to the magnetic field stimulus through the electric field transducers of the electromagnetic transducer array; c') establishment of levels; d') establishment of a tolerance (NT) for the reference level established in step (c'); e') determination of the lowest tissue stimulation frequencies (fbx) as the point where the tissue impedance response falls below the tolerance (NT) established in step (d');f') determination of the upper tissue stimulation frequencies (ftx) as the point where the tissue impedance response; Petition 870260051474, dated 05 / 28 / 2026, page 20 / 94 6 / 58 returns to the tolerance (NT) established in step (d'); where the higher tissue stimulation frequencies (ftx) are greater than the lower tissue stimulation frequencies (fbx) ex is a natural number greater than or equal to 1.

[0011] In addition, this disclosure includes other means of tissue stimulation with combinations of magnetic fields and electric fields with feedback to dynamically adjust the electromagnetic stimulation signals.

[0012] The device for stimulating a tissue with electromagnetic fields, the device consists of: a computing unit; an external power source connected to the computing unit; a decoupling circuit connected to the external power source and the computing unit; an array of electromagnetic transducers connected to the computing unit and the decoupling circuit; wherein the computing unit implements a method for generating an activation signal that is received by the array of electromagnetic transducers through the decoupling circuit.

[0013] There has long been a need to improve the efficiency of tissue stimulation to avoid overstimulation and to adapt the stimulation according to each particular tissue. The methods disclosed in this document solve this problem by providing feedback on the tissue stimulation response by dynamically adjusting the stimulation signal. BRIEF DESCRIPTION OF THE FIGURES

[0014] FIG. 1 shows an example of a transducer arrangement over a volume containing a tissue.

[0015] FIG. 2A shows an example of the placement of an array of electromagnetic transducers in contact with and targeting an individual's arm tissue. Petition 870260051474, dated 05 / 28 / 2026, page 21 / 94 7 / 58

[0016] FIG. 2B shows an example of the placement of a non-contact electromagnetic transducer array targeting an individual's arm tissue.

[0017] FIG. 3A shows an example of the placement of an array of electromagnetic transducers in contact with and targeting tissue in the abdominal area of ​​an individual.

[0018] FIG. 3B shows an example of the placement of a non-contact electromagnetic transducer array targeting tissue in the abdominal area of ​​an individual.

[0019] FIG. 4A shows an example of the placement of an array of electromagnetic transducers in contact with and targeting tissue in the knee area of ​​an individual.

[0020] FIG. 4B shows an example of the placement of a non-contact electromagnetic transducer array targeting tissue in the knee area of ​​an individual.

[0021] FIG. 5A shows an example of the frequency variation of a stimulation signal over time.

[0022] FIG. 5B shows an example of a sinusoidal wave activation signal.

[0023] FIG. 5C shows an example of a square wave activation signal.

[0024] FIG. 6 shows an example of an alternative square wave activation signal with a duty cycle variation.

[0025] FIG. 7 shows an example of an activation signal in the form of an alternating triangular wave.

[0026] FIG. 8 shows an example of a segmented activation signal that combines alternating ramp signals with alternating square wave signals.

[0027] FIG. 9A shows an example of impedance response of Petition 870260051474, dated 05 / 28 / 2026, page 22 / 94 8 / 58 bandpass-shaped tissue with frequency sweep electromagnetic stimulation.

[0028] FIG. 9B shows an example of an approximate representation of the bandpass tissue impedance response with frequency sweep electromagnetic stimulation.

[0029] FIG. 9C shows an example of tissue impedance response in the form of a low-pass response to a frequency-sweep electromagnetic stimulation.

[0030] FIG. 9D shows an example of an approximate representation of the tissue impedance response in the form of a low-pass signal to a frequency-sweep electromagnetic stimulation.

[0031] FIG. 10A shows an example of a bandpass tissue impedance response with four frequency-sweep electromagnetic stimulation bands.

[0032] FIG. 10B shows an example of an approximate representation of the four-bandpass tissue impedance response of a frequency-sweep electromagnetic stimulation.

[0033] FIG. 11A shows a photograph of an example of a type of malignant tissue in the neck of an individual before being stimulated with a frequency sweep of electromagnetic fields.

[0034] FIG. 11B shows an example of a type of malignant tissue in the neck of the same individual as in FIG. 11A, after being stimulated with a frequency sweep of electromagnetic fields.

[0035] FIG. 11C shows a tissue impedance signal that corresponds to the tissue impedance response through a single electric field channel of the individual at an intermediate step between FIG. 11A and FIG. 11B.

[0036] FIG. 11D shows a smoothed tissue impedance signal Petition 870260051474, dated 05 / 28 / 2026, page 23 / 94 9 / 58 of the tissue impedance signal shown in FIG. 11C.

[0037] FIG. 12 shows a flow diagram of the method for tissue stimulation with frequency-sweeping electric fields.

[0038] FIG. 13 shows a flow diagram of the method for tissue stimulation with frequency-sweeping electric fields with additional steps.

[0039] FIG. 14 shows a flow diagram of the method for tissue stimulation with frequency-sweeping electric fields in which the k-step is disaggregated.

[0040] FIG. 15 shows a method flow diagram for tissue stimulation with frequency-sweeping magnetic fields.

[0041] FIG. 16 shows a flow diagram of the method for tissue stimulation with frequency-sweeping electric fields with additional steps in which the k-step is disaggregated.

[0042] FIG. 17 shows a block diagram of an example of a tissue stimulation device of this disclosure.

[0043] FIG. 18 shows a block diagram of an example of a special-purpose computing unit of a tissue stimulation device of this disclosure. DETAILED DESCRIPTION

[0044] This disclosure reveals methods for frequency scanning of electric and magnetic fields, or combinations thereof, applied to a tissue and a device for stimulation of electric and magnetic fields. The first method for frequency scanning of electric and magnetic fields consists of tissue stimulation using electric fields which includes the following steps: a) applying an electric field stimulus to a tissue Petition 870260051474, dated 05 / 28 / 2026, page 24 / 94 10 / 58 through an array of electromagnetic transducers that receives an activation signal, whose frequency varies from an initial tissue stimulation frequency (fie) to a final tissue stimulation frequency (ffe) with increments or decrements in the steps of a frequency delta (Δfe) during a time delta (Δte); b) measurement of the tissue impedance response to the stimulus from step (a); c) establishing a reference level with the tissue impedance response measured in step (b); d) establishing a tolerance (NT) to the reference level established in step (c); (e) determination of lower tissue stimulation frequencies (fbx) as the point at which the tissue impedance response falls below the tolerance (NT) established in step (d); f) determination of upper tissue stimulation frequencies (ftx) as the point at which the tissue impedance response returns to the tolerance (NT) established in step (d); characterized by the stimulation frequencies of the upper tissue (ftx) being greater than the stimulation frequencies of the lower tissue (fbx) and “x” being a natural number greater than or equal to 1.

[0045] Tissue refers to the biological tissues of living beings composed of one or more cells. They can be made up of cells of only one class, all alike, or of several types of cells arranged in an orderly fashion to form an organ or an organism. The tissue mentioned can be healthy tissue, such as epithelial tissue, connective tissue, muscle tissue, muscle bundle, nervous tissue, or a combination thereof. Tissue can also be tissue with a total or partial biochemical imbalance in healthy tissue; this biochemical imbalance, in turn, can correspond to benign tissue, neoplastic tissue, or malignant neoplastic tissue. Petition 870260051474, dated 05 / 28 / 2026, page 25 / 94 11 / 58 or any cell outside or in homeostasis. Furthermore, the tissue may refer to cells in vivo or prior to the implantation of said cells in an in vivo environment.

[0046] The tissue may come from or be from animals, including but not limited to: mammals, avian species, including chickens, turkeys, geese and ducks; fish, crustacean species (shrimp, lobsters, crayfish); and reptiles such as crocodiles and alligators. The term mammal used herein refers to any mammal classified as a mammal, including humans, non-human primates such as cynomolgus monkeys, chimpanzees, baboons and gorillas; domestic and farm animals, including equine, bovine, swine, caprine, canine, feline, ovine, rabbit, and llama species; ungulates such as cattle, sheep, swine, equines, and goats; canines, felines, murines, and rabbits; and rodents such as guinea pigs, hamsters and rats.

[0047] Stimulation of a biological tissue refers to the administration of energy to that biological tissue in order to induce certain changes in the characteristics of that tissue, such as tissue impedance response, tissue vascularization, tissue temperature, tissue health, tissue growth rate, among others.

[0048] With reference to FIG. 17, an example of a tissue stimulation device of the present disclosure is shown; the tissue stimulation device consists of a computing unit (21), an external power source (22) connected to the computing unit (21), a decoupling circuit (23) connected to the external power source (22) and to the computing unit (21), an array of electromagnetic transducers (1) connected to the computing unit (21) and to the decoupling circuit (23); the computing unit (21) implements the tissue stimulation method with a frequency-sweeping electric field, the tissue stimulation method with a frequency-sweeping magnetic field, and methods combining stimulation with electric fields and magnetic fields. Petition 870260051474, dated 05 / 28 / 2026, page 26 / 94 12 / 58 magnetic and can be configured with the tissue stimulation device in order to generate activation signals that receive the electromagnetic field, electric field or magnetic field transducers through the decoupling circuit (23).

[0049] This control system can also be understood as a device for stimulating a tissue, a device for stimulating a tissue with electromagnetic fields, or simply a device for stimulating a tissue.

[0050] Referring to FIG. 18, an example of the computing unit (21) being a special computing unit consisting of a central processing unit (CPU) (21a) connected to oscillators of a first OSC 1 (21b), a second oscillator OSC 2 (21c) to an oscillator OSC n (21d), each oscillator with activation signal outputs (31, 32 and 33), where n is a natural number equal to or greater than zero, according to this the computing unit (21) can have a maximum of n activation signal outputs. The activation signal outputs are also referred to as channels.

[0051] Optionally, the activation signal output (31, 32 and 33) of each oscillator is connected to the electromagnetic transducer array (1) directly or via a decoupling circuit (23). Alternatively, the CPU (21a) is also connected to a peripheral device selected from among others, from storage devices such as a memory unit, a database and a hard disk, input devices such as a keyboard, a camera, a touchscreen monitor and a scanner, output devices such as a monitor and a printer.

[0052] In another example of a tissue stimulator device, the oscillators are replaced by signal generators.

[0053] Optionally, the parameters of each activation signal such Petition 870260051474, dated 05 / 28 / 2026, page 27 / 94 13 / 58 such as frequency, phase, amplitude, duty cycle, can be modified from instructions from a remote computing unit, by a user through a HID connected to the tissue stimulation device.

[0054] The computing unit (21) of the tissue stimulation device can use feedback (30), for example, feedback from the tissue impedance response to dynamically adjust the outputs of the activation signals (31, 32 and 33), which are received by the transducers and applied to the tissue to stimulate it.

[0055] Feedback (30) is a mechanism by which a certain part of the output of a system is redirected back to the input, with the aim of controlling its behavior. For example, when stimulating tissue with electric fields, or magnetic fields, or both fields, there may be a variation in the tissue impedance response, tissue impedance response feedback can be used employing electric field transducers, which makes it possible to check the variations in the tissue impedance response and dynamically adjust the activation signal.

[0056] Alternatively, feedback is not limited to obtaining the tissue impedance response to tissue stimulation. Feedback can incorporate, for example, temperature measurement in order to determine tissue fatigue, tissue surface imaging in order to determine tissue vascularization, tissue impedance response measurements, or combinations thereof.

[0057] By stimulating tissue with electric, magnetic, or both fields, there may be increases in the tissue surface temperature; a temperature sensor or a temperature measuring device can be used to check temperature variations and dynamically adjust the activation signal of the electric, magnetic, or both field stimuli, for example, to avoid tissue damage from overheating. Petition 870260051474, dated 05 / 28 / 2026, page 28 / 94 14 / 58

[0058] For the purposes of this disclosure, a computing unit is a device that processes data, for example, microcontrollers, microprocessors, DSCs (Digital Signal Controllers), FPGAs (Field Gate Arrays), CPLDs (Complex Programmable Logic Devices), ASICs (Application-Specific Integrated Circuits), SoCs (Systems on Chip), PSoCs (Programmable Systems on Chip), computers, servers, tablets, cell phones, smartphones, and computing units known to those qualified in the state of the art, and combinations thereof. This computing unit may include a storage device, a monitor, and / or a Human Interface Device (HID), and may be, or include, a special-purpose computing unit programmed to perform the method of this disclosure.

[0059] A storage device includes, without limitation, RAM (cache memory, SRAM, DRAM, DDR), ROM memory (Flash, cache, HDD, SSD, EPROM, EEPROM, removable ROM memory (SD (miniSD, microSD, etc.), MMC (MultiMedia Card), Compact Flash, SMC (Smart Media Card), SDC (Secure Digital Card), MS (Memory Card), among others)), CD-ROM, Digital Versatile Disc (DVD) or other optical storage units, magnetic cassettes, magnetic tapes, or any other storage medium that can be used to store information and that can be accessed through the computing unit, among others known to those skilled in the art, and combinations thereof. The storage device has memory registers in which software modules, instructions, and data structures are stored.

[0060] A monitor includes, without limitation, monitors capable of being connected to a computing unit, displaying the output. CRT monitor, flat panel monitor, Liquid Crystal Display (LCD), matrix LCD Petition 870260051474, dated 05 / 28 / 2026, page 29 / 94 Active 15 / 58 LCD, passive matrix LCD, LED monitors, projector monitors, TVs (4KTV, HDTV, Plasma TV, Smart TV), OLED monitors, AMOLED monitors, quantum dot (QD) monitors, segment monitors, among other devices capable of displaying data to the user, known to those familiar with the art, and combinations thereof.

[0061] A HID includes, without limitation, keyboard, mouse, trackball, touchpad, pointing stick, joystick, touch screen, among other devices capable of allowing the user to input data into the computing unit of the tissue stimulation device, known to those skilled in the art, and combinations thereof.

[0062] The decoupling circuit makes it possible to electrically decouple the external power source from the electromagnetic transducer array; this circuit may be based on optocouplers, relays, operational amplifiers, resistors, capacitors, transformers, combinations of diodes of these and other electronic elements to electrically decouple two circuits or electrical elements.

[0063] The external power source makes it possible to supply the electrical energy necessary for the operation of the electromagnetic transducer array and may be a device capable of maintaining a potential difference between two or more terminals, such as an alternating current power source, a direct current power source, batteries, photovoltaic power source, thermoelectric power source, among other devices capable of maintaining a voltage between two or more terminals known to those skilled in the art, or combinations thereof.

[0064] The activation signal received by the transducers of the electromagnetic transducer array (1), of electric field or magnetic field, may be a signal selected from a direct or alternating current signal, a pulsed signal, a series of alternating or non-alternating impulse signals. Petition 870260051474, dated 05 / 28 / 2026, page 30 / 94 Alternating 16 / 58 signals, a square wave signal with varying duty cycle, a triangular wave signal, a sawtooth wave signal, amplitude modulation (AM), frequency modulation (FM), phase modulation (PM), pulse position modulation (PPM), pulse width modulation (PWM), and combinations thereof. These signals are generated by a computing unit or by a signal generator or combinations thereof, according to programs and feedback.

[0065] The programs mentioned in this disclosure correspond to information, encoded or not, in a computing unit and which modify all parameters of the activation signal that activates the transducer array (1).

[0066] Signal generators can be selected from the group of professional wave generators, integrated DDS (Direct Digital Synthesizer) / DAC (Digital to Analog Conversion) synthesizer circuits, NCO (Numerically Controlled Oscillator), operational amplifier arrays in wave generator configuration, bistable oscillator circuits and combinations of the above mentioned. The signal generator may also be named as a wave generator.

[0067] In addition, the computing unit (21) makes it possible for one or more activation signals to be applied to each transducer at a given time, sequentially, out of phase with respect to the other activation signal or to several stimulation signals, randomly or according to a program set for each of the transducers.

[0068] The activation signal that activates the transducers of the electromagnetic transducer array (1) can be understood as an electromagnetic stimulation signal, an electrical stimulation signal when the predominant phenomenon is the electric field, a magnetic stimulation signal when the predominant phenomenon is the magnetic field.

[0069] To activate the electric field transducers, the signal of Petition 870260051474, dated 05 / 28 / 2026, page 31 / 94 17 / 58 activation can be selected from, among other things, direct or alternating current signal, pulsed signal, a train of alternating or non-alternating impulse signals, square wave signal with duty cycle variation, triangular wave signal, sawtooth wave signal, amplitude modulation (AM), frequency modulation (FM), phase modulation (PM), pulse position modulation (PPM), pulse width modulation (PWM), and combinations thereof.

[0070] In step a) of the method of this disclosure, the method begins with the stimulation of the tissue by applying a signal of a certain frequency for a specific period of time which may be limited by a user or may be programmed into a computing unit. For example, the method may begin with a 1 Hz fiede applied for a Δte of 1 second, increasing the fiede frequency by Δfe equal to 1 Hz, applying the new fiede of 2 Hz for a Δte of 1 second, and continuously increasing by the same Δfe, applying the new fiede for a Δte of 1 second.

[0071] An array of electromagnetic transducers may be a set of n electric field transducers or magnetic field transducers, or a combination thereof, where n is a natural number greater than or equal to 1.

[0072] These electromagnetic transducers may be designated as electromagnetic field transducers, electric field transducers, or magnetic field transducers, or they may be configured as a combination of electric field and magnetic field transducers. Magnetic field transducers may also be designated as magnetic transducers, and electric field transducers may also be designated as electric transducers. If the electric field is the predominant phenomenon, such electromagnetic transducers are understood to be electric field transducers; conversely, when the predominant phenomenon is the magnetic field, they are understood to be... Petition 870260051474, dated 05 / 28 / 2026, page 32 / 94 18 / 58 electromagnetic transducers are magnetic field transducers.

[0073] The transducers in the electromagnetic transducer array have an active face in different shapes that can be selected from, among others, a group of geometric figures such as squares, rectangles, circles, ovals, concentric rings and combinations thereof, so that they cover different areas of the outer surface of a volume containing the tissue of interest.

[0074] The active face of a transducer is the surface of the transducer through which the electric field signal, magnetic field signal, or electromagnetic field signal has the greatest intensity.

[0075] Electric field transducers are selected, among others, from the group consisting of motors, electrodes, photoelectric transducers, electric induction actuators, conductive plates that generate electric fields, antennas, or combinations thereof. Magnetic field transducers are selected, among others, from the group consisting of motors, electrodes, magnetic induction actuators, magnetic field generating coils with or without a core, electromagnets, antennas, and combinations thereof.

[0076] With reference to FIG. 1, for example, an arrangement of electromagnetic transducers (1) is placed on the surface of a volume (2), the arrangement of which consists from an electromagnetic transducer (1a) to an electromagnetic transducer (1e'), which, in the illustrated example, consists of pairs of transducers, a first pair of electromagnetic transducers composed of a transducer (1a) and a transducer (1a'), a second pair of electromagnetic transducers, composed of a transducer (1b) and a transducer (1b'), a third pair of electromagnetic transducers composed of a transducer (1c) and a transducer (1c'), a fourth pair of electromagnetic transducers composed of a transducer (1d) and a transducer (1d'), a fifth pair of electromagnetic transducers composed of a transducer (1e) and a transducer (1e'). Each pair of transducers faces each other and each Petition 870260051474, dated 05 / 28 / 2026, page 33 / 94 19 / 58 one is oriented with its active face in such a way that it aims at the interior of the volume (2) containing the tissue (3) of interest. Optionally, the active faces of the transducers are directed towards the tissue (3).

[0077] Furthermore, arrangement (1) optionally satisfies an orthogonality condition insofar as a plane parallel to any of the surfaces of the active faces of the first pair of transducers is orthogonal to another plane parallel to any of the surfaces of the active faces of the second, third, fourth and fifth pairs of transducers and, furthermore, any plane parallel to the surfaces of the active faces of the second pair of transducers is orthogonal to any other plane parallel to any of the surfaces of the active faces of the third, fourth and fifth pairs of transducers and, furthermore, any plane parallel to the surfaces of the active faces of the second pair of transducers is orthogonal to any other plane parallel to any of the surfaces of the active faces of the third, fourth and fifth pairs of transducers and, furthermore,Any plane parallel to the surfaces of the active faces of the third pair of transducers is orthogonal to any other plane parallel to any of the surfaces of the active faces of the fourth and fifth pairs of transducers, and also any plane parallel to the surfaces of the active faces of the fourth pair of transducers is orthogonal to any other plane parallel to any of the surfaces of the active faces of the fifth pair of transducers. Furthermore, the projections of the planes of the active faces point towards the maximum possible tissue coverage of said tissue, with this configuration ensuring optimal tissue stimulation.

[0078] It may happen that the transducers that make up each pair of transducers are not completely aligned or parallel to each other, or that they do not preserve the orthogonality condition of the transducers described in the previous paragraph, but may still be able to stimulate the tissue (3).

[0079] In one example of this disclosure, the active face of Petition 870260051474, dated 05 / 28 / 2026, page 34 / 94 20 / 58 electric field transducers in the electromagnetic transducer arrangement (1) are in contact with the outer surface of the tissue (3). In this way, less electrical energy is required for the operation of the electric field transducers compared with the alternative where the electric field transducers are located at a certain distance from the outer surface of the tissue (3).

[0080] In another example, the active face of the transducers that make up the electromagnetic transducer array (1) is separated by a certain distance from the outer surface of the tissue (3) (necessary, for example, when it is not possible to make physical contact with the outer surface of the tissue). In this way, more electrical energy will be required for the operation of the electric field transducers compared with the alternative where the electric field transducers are in contact with the outer surface of the tissue (3).

[0081] Alternatively, the active face of the transducers that make up the electromagnetic transducer array (1) is separated by a certain distance from the outer surface of the tissue (3) and a second group of transducers of the electromagnetic transducer array (1) is in contact with the outer surface of the tissue (3). This mixed configuration of transducer positioning makes it possible, for example, to efficiently reach the tissue (3) found in the volume (2) where the surface of said tissue (3) varies in such a way that some areas tolerate physical contact with the active face of the transducer and other areas are difficult to access or do not tolerate such physical contact.

[0082] Optionally, in step (a), the active face of at least one of the electric field transducers in the electromagnetic transducer array (1) is in contact with the outer surface of the tissue (3).

[0083] The projections of the active face planes of the transducers are arranged in the direction of the tissue (3) and cover the maximum surface area. Petition 870260051474, dated 05 / 28 / 2026, page 35 / 94 21 / 58 possible of the said tissue (3), ensuring optimal stimulation of this in this configuration.

[0084] In addition, it is possible that the transducers are not perfectly aligned or parallel to each other.

[0085] The intensity and direction parameters of an electric field vector towards the interior of volume (2) depend on the arrangement of the transducers around volume (2) containing the tissue (3). For example, with an arrangement of electric field transducers, if the active face is in contact with the surface of volume (2), then the electric field intensity will be between 2 V / cm and 5 V / cm. If, on the other hand, the electric field transducers are located at a defined distance from the surface, then the value of the electric field intensity will be between 330 V / cm and 20 kV / cm for distances between 0.01 cm and 50 cm, and optionally between 0.01 cm and 4 cm.

[0086] Alternatively, the value of the electric field intensity for transducers with the active face in contact with the tissue surface can be selected from 2 V / cm to 5 V / cm, from 2.1 V / cm to 4.9 V / cm, from 2.2 V / cm to 4.8 V / cm, from 2.3 V / cm to 4.7 V / cm, from 2.4 V / cm to 4.6 V / cm, from 2.5 V / cm to 4.5 V / cm, from 2.6 V / cm to 4.4 V / cm, from 2.7 V / cm to 4.3 V / cm, from 2.8 V / cm to 4.2 V / cm, from 2.9 V / cm to 4.1 V / cm, from 3 V / cm to 4 V / cm, from 3.1 V / cm to 3.9 V / cm, from 3.2 V / cm to 3.8 V / cm, from 3.3 V / cm to 3.7 V / cm, from 3.4 V / cm to 3.6 V / cm, from 2.2 V / cm to 5 V / cm, from 2.4 V / cm to 5 V / cm, from 2.6 V / cm to 5 V / cm, from 2.8 V / cm to 5 V / cm, from 3 V / cm to 5 V / cm, from 3.2 V / cm to 5 V / cm, from 3.4 V / cm to 5V / cm, 3.6 V / cm to 5 V / cm, from 3.8 V / cm to 5 V / cm, from 4 V / cm to 5 V / cm, from 4.2 V / cm to 5 V / cm, from 4.4 V / cm to 5 V / cm, from 4.6 V / cm to 5 V / cm, from 4.8 V / cm to 5 V / cm, from 2 V / cm to 4.8 V / cm, from 2 V / cm to 4.6 V / cm, from 2 V / cm to 4.4 V / cm, from 2 V / cm to 4.2 V / cm, from 2 V / cm to 4 V / cm, from 2 V / cm to 3.8 V / cm, from 2 V / cm to 3.6 V / cm, from 2 V / cm to 3.4 V / cm, from 2 V / cm to 3.2 V / cm, from 2 V / cm to 3 V / cm, from 2 V / cm to 2.8 V / cm, 2 V / cm to 2.6 V / cm, 2 V / cm to 2.4 V / cm, 2 V / cm to 2.2 V / cm, 2.2 V / cm to 2.4 V / cm, 2.4. Petition 870260051474, dated 05 / 28 / 2026, page 36 / 94 22 / 58 V / cm to 2.6 V / cm, 2.6 V / cm to 2.8 V / cm, 2.8 V / cm to 3 V / cm, 3 V / cm to 3.2 V / cm, 3.2 V / cm to 3.4 V / cm, 3.4 V / cm to 3.6 V / cm, 3.6 V / cm to 3.8 V / cm, 3.8 V / cm to 4 V / cm, 4 V / cm to 4.2 V / cm, 4.2 V / cm to 4.4 V / cm, 4.4 V / cm to 4.6 V / cm, 4.6 V / cm to 4.8 V / cm, 4.8 V / cm to 5 V / cm.

[0087] Optionally, the value of the electric field strength for transducers located at a defined distance from the tissue surface can be selected from a range of 0.33 kV / cm to 20 kV / cm, 0.83 kV / cm to 19.5 kV / cm, 1.33 kV / cm to 19 kV / cm, 1.83 kV / cm to 18.5 kV / cm, 2.33 kV / cm to 18 kV / cm, 2.83 kV / cm to 17.5 kV / cm, 3.33 kV / cm to 17 kV / cm, 3.83 kV / cm to 16.5 kV / cm, 4.33 kV / cm to 16 kV / cm, 4.83 kV / cm to 15.5 kV / cm, 5.33 kV / cm to 15 kV / cm, from 5.83 kV / cm to 14.5 kV / cm, from 6.33 kV / cm to 14 kV / cm, from 6.83 kV / cm to 13.5 kV / cm, from 7.33 kV / cm to 13 kV / cm, from 7.83 kV / cm to 12.5 kV / cm, from 8.33 kV / cm to 12 kV / cm, from 8.83 kV / cm to 11.5 kV / cm, from 9.33 kV / cm to 11 kV / cm, from 9.83 kV / cm to 10.5 kV / cm, from 1.33 kV / cm to 20 kV / cm, from 2.33 kV / cm to 20 kV / cm, from 3.33 kV / cm at 20 kV / cm, from 4.33 kV / cm to 20 kV / cm, from 5.33 kV / cm to 20 kV / cm, from 6.33 kV / cm to 20 kV / cm, from 7.33 kV / cm to 20 kV / cm, from 8.33 kV / cm to 20 kV / cm, from 9.3 3 kV / cm to 20 kV / cm, from 10.33 kV / cm to 20 kV / cm, from 11,33 kV / cm to 20 kV / cm, from 12.33 kV / cm to 20 kV / cm, from 13.33 kV / cm to 20 kV / cm, from 14.33 kV / cm to 20 kV / cm, from 15.33 kV / cm to 20 kV / cm, from 16.33 kV / cm to 20 kV / cm, from 17.33 kV / cm to 20 kV / cm, from 18.33 kV / cm to 20 kV / cm, from 19.33 kV / cm to 20 kV / cm, from 0.33 kV / cm to 19 kV / cm, from 0.33 kV / cm to 18 kV / cm, from 0.33 kV / cm to 17 kV / cm, from 0.33 kV / cm a 16 kV / cm, from 0.33 kV / cm to 15 kV / cm, from 0.33 kV / cm to 14 kV / cm, from 0.33 kV / cm to 13 kV / cm, from 0.33 kV / cm to 12 kV / cm, from 0.33 kV / cm to 11 kV / cm, from 0.33 kV / cm to 10 kV / cm, from 0.33 kV / cm to 9 kV / cm, from 0.33 kV / cm to 8 kV / cm, from 0.33 kV / cm to 7 kV / cm, from 0.33 kV / cm to 6 kV / cm, from 0.33 kV / cm to 5 kV / cm, from 0.33 kV / cm to 4 kV / cm, from 0.33 kV / cm at 3 kV / cm, from 0.33 kV / cm to 2, Petition 870260051474, dated 05 / 28 / 2026, page 37 / 94 23 / 58 kV / cm, from 0.33 kV / cm to 1 kV / cm, from 1.33 kV / cm to 2.33 kV / cm, from 2.33 kV / cm to 3.33 kV / cm, from 3.33 kV / cm to 4.33 kV / cm, from 4.33 kV / cm to 5.33 kV / cm, from 5.33 kV / cm to 6.33 kV / cm, from 6.33 kV / cm to 7.33 kV / cm, from 7.33 kV / cm to 8.33 kV / cm, from 8.33 kV / cm to 9.33 kV / cm, from 9.33 kV / cm to 10.33 kV / cm, from 10.33 kV / cm to 11.33 kV / cm, 11.33 kV / cm a 12.33 kV / cm, of 12.33 kV / cm a 13.33 kV / cm, of 13.33 kV / cm a 14.33 kV / cm, of 14.33 kV / cm a 15.33 kV / cm, of 15.33 kV / cm a 16.33 kV / cm, of 16.33 kV / cm a 17.33 kV / cm, of 17.33 kV / cm a 18.33 kV / cm, of 18.33 kV / cm a 19.33 kV / cm, of 19.33 kV / cm a 20 kV / cm.

[0088] Alternatively, the transducers may be located at a distance from the tissue surface, at a distance selected from a range of 0.01 cm to 50 cm, 2 cm to 48 cm, 4 cm to 46 cm, 6 cm to 44 cm, 8 cm to 42 cm, 10 cm to 40 cm, 10 cm to 40 cm, 12 cm to 38 cm, 14 cm to 36 cm, 16 to 34 cm, 18 to 32 cm, 20 to 30 cm, 22 to 28 cm, 22 to 28 cm, 24 to 26 cm, 5 to 50 cm, 10 cm to 50 cm, 15 cm to 50 cm, 20 cm to 50 cm, 25 cm to 50 cm, 30 cm to 50 cm, from 35 cm to 50 cm, from 40 cm to 50 cm, from 40 cm to 50 cm, from 45 cm to 50 cm, from 0.01 cm to 45 cm, from 0.01 cm to 40 cm, from 0.01 cm to 35 cm, from 0.01 cm to 30 cm, from 0.01 cm to 25 cm, from 0.01 cm to 25 cm, from 0.01 cm to 20 cm, from 0.01 cm to 15 cm, from 0.01 cm to 10 cm, from 0.01 cm to 5 cm, from 5 cm to 10 cm, from 10 cm to 15 cm, from 15 cm to 20 cm, from 15 cm to 20 cm, from 20 cm to 25 cm, from 25 cm to 30 cm, from 30 cm to 35 cm, from 35 cm to 40 cm, from 40 cm to 45 cm, from 45 cm to 50 cm.

[0089] In another example of this disclosure, in step (a), the arrangement of electromagnetic transducers (1) has at least two electric field transducers and at least two of said transducers are activated simultaneously by means of a frequency sweep for a determined period of time.

[0090] Alternatively, the electromagnetic transducers of the arrangement (1) are arranged on a structure (4) that encloses the volume (2) and whose Petition 870260051474, dated 05 / 28 / 2026, page 38 / 94 24 / 58 The objective is to provide a support structure for electric field transducers that are arranged with the active face pointing towards the tissue of interest. The structure (4) can also be used to alter the shape of the volume surface (2) in order to obtain a flat surface that allows adjusting the position of the electric field transducers, so that an ideal electric field intensity is obtained for tissue stimulation (3). The structure (4) can be supported on the same tissue or mechanically, on a fixed or mobile base. The type of frame (4) can be chosen from the group consisting of shirts, vests, gloves, helmets, glasses, suspenders, socks, boots, shoes, scarves, collars and other structures that provide support for transducers and combinations thereof. In addition, the frame (4) can cover the volume (2) totally or partially.

[0091] Optionally, the base on which the frame (4) is positioned may be movable in order to enable movement of the array (1) relative to the surface of the volume (2) and thus achieve different volumes from different external points and to vary the electric field vector.

[0092] FIG. 2A illustrates the arrangement of an array of electromagnetic transducers (1) on a volume (2) consisting of an individual's arm. Inside the arm there is a tissue (3) that is to be stimulated electromagnetically. The said array of electromagnetic transducers (1) consists of two groups of transducers, as detailed below:

[0093] A first group of transducers consisting of two pairs of electromagnetic transducers. A first pair of electromagnetic transducers: a transducer (1f) and a transducer (1f'), a second pair of electromagnetic transducers: a transducer (1g) and a transducer (1g'), the first pair and the second pair of electromagnetic transducers are arranged radially around the humerus, with their active faces in contact with the skin surface.

[0094] A second group of transducers consisting of a pair of Petition 870260051474, dated 05 / 28 / 2026, page 39 / 94 25 / 58 electromagnetic transducers: one transducer (1h) and one transducer (1h'), said pair of electromagnetic transducers is arranged on a plane normal to the axis of the humerus, the active face of transducer (1h) on the shoulder and in contact with the skin, and the active face of transducer (1h') on the elbow and in contact with the skin.

[0095] Each pair of transducers is arranged in such a way that the active faces of the transducers that make up this pair are partially facing each other, aligned with their active faces in the direction of the tissue position (3) and with their active faces in contact with the skin.

[0096] Furthermore, arrangement (1) optionally satisfies an orthogonality condition wherein a plane parallel to any of the surfaces of the active faces of the first pair of transducers is orthogonal to another plane parallel to any of the surfaces of the active faces of the second and third pair of transducers and, furthermore, any plane parallel to the surfaces of the active faces of the second pair of transducers is orthogonal to any other plane parallel to any of the surfaces of the active faces of the first and third pair of transducers and, furthermore, the projections of the planes of the active faces pointed at the tissue cover the maximum possible surface of said tissue, with this configuration ensuring optimal tissue stimulation.

[0097] It may also be possible that the transducers that make up each pair of transducers are not completely aligned or parallel to each other, or do not preserve the orthogonality condition of the transducers described in the previous paragraph, and may also be able to stimulate the tissue (3).

[0098] There are several diagnostic tools for learning the location of the tissue (3), for example: magnetic resonance imaging, computed tomography, PET scan (Positron Emission Tomography), x-rays, Doppler ultrasound, electrocardiograms, palpation diagnosis, marking Petition 870260051474, dated 05 / 28 / 2026, page 40 / 94 26 / 58 with an arrow, among others.

[0099] It may also be possible to learn the location of the tissue (3) using a measure of the tissue impedance response.

[00100] FIG. 2B illustrates a similar arrangement of the transducers, but where the active faces of the transducers are at a distance between 0.01 cm and 50 cm from the surface of the individual's skin, supported on a structure (4) and, optionally, between 0.01 cm and 4 cm.

[00101] FIG. 3A illustrates the arrangement of an array of electromagnetic transducers (1) on a volume (2) consisting of the abdomen of an individual. Inside the abdomen, there is a tissue (3) that is to be stimulated electromagnetically. The said array of electromagnetic transducers (1) comprises five groups of transducers, as detailed below:

[00102] A first pair of transducers: a transducer (1i) and a transducer (1i'), a second pair of transducers: a transducer (1j) and a transducer (1j'), a third pair of transducers: a transducer (1k) and a transducer (1k'), a fourth pair of transducers: a transducer (1l) and a transducer (1l').

[00103] The aforementioned pairs of transducers are arranged radially around an axis parallel to the spine, over the abdominal and dorsal area, so that the active faces of the transducers face each other and in the direction of the tissue position (3) and with their active faces in contact with the skin.

[00104] The projections of the active faces of the transducers are arranged in the direction of the tissue and cover the maximum possible surface area of ​​said tissue, with this configuration guaranteeing ideal tissue stimulation.

[00105] Furthermore, it is possible that the transducers comprising each pair are not completely aligned or parallel to each other.

[00106] FIG. 3B illustrates a similar arrangement of the transducers, Petition 870260051474, dated 05 / 28 / 2026, page 41 / 94 27 / 58 but where the active faces of the transducers are at a distance between 0.01 cm and 50 cm from the surface of the individual’s skin, supported on a structure (4) and, optionally, between 0.01 cm and 4 cm.

[00107] FIG. 4A illustrates the arrangement of an array of electromagnetic transducers (1) on a volume (2) consisting of an individual's knee. Inside the knee there is a tissue (3) that is to be stimulated electromagnetically. The said array of electromagnetic transducers (1) comprises two pairs of transducers, as detailed below:

[00108] A first pair of transducers: a transducer (1m) and a transducer (1m') and a second pair of transducers: a transducer (1n) and a transducer (1n').

[00109] The aforementioned pairs of transducers are arranged around the knee at the position of the tissue (3) at the level of the patella and in such a way that the active faces of the transducers face each other in the direction of the position of the tissue (3).

[00110] Furthermore, arrangement (1) optionally satisfies an orthogonality condition wherein a plane parallel to any of the surfaces of the active faces of the first pair of transducers is orthogonal to another plane parallel to any of the surfaces of the active faces of the second and third pair of transducers and, furthermore, any plane parallel to the surfaces of the active faces of the second pair of transducers is orthogonal to any other plane parallel to any of the surfaces of the active faces of the first and third pair of transducers and, furthermore, the projections of the planes of the active faces pointed at the tissue cover the maximum possible surface of said tissue, with this configuration ensuring optimal tissue stimulation.

[00111] It may also be possible that the transducers comprising each pair of transducers are not completely aligned or parallel to each other, or do not preserve the orthogonality condition of the transducers described in the previous paragraph, and may also stimulate the Petition 870260051474, dated 05 / 28 / 2026, page 42 / 94 28 / 58 fabric (3).

[00112] FIG. 4B illustrates a similar arrangement of the transducers, but where the active faces of the transducers are at a distance between 0.01 cm and 50 cm from the surface of the individual's skin, supported on a structure (4) and, optionally, between 0.01 cm and 4 cm.

[00113] Moving on to step (b) of the method of this disclosure, step (b) consists of measuring the stimulated tissue impedance response from step (a). When the frequency sweep is applied to the tissue, the tissue responds with a variation of its parameters which are measured, optionally, using the same electromagnetic transducers. This measurement of the stimulated tissue acts as feedback and allows dynamically altering the signal characteristics from step (a).

[00114] Subsequently, step (c) of this method allows establishing a reference level with the tissue impedance response measured in step (b). This reference level can be established by a user or determined as a maximum tissue impedance response over a given time.

[00115] Continuing with step (d) of this method, this step consists of defining a tolerance (NT) at the reference level established in step (c). This NT corresponds to a percentage value of the reference level established in step (c) and can be defined in a computing unit or entered by a user.

[00116] In an example of the method, in an example, in step (d'), the NT can be between 5% and 60% and, optionally, between 25% and 50%.

[00117] Optionally, the NT can be selected from a range of 5% to 10%, 10% to 15%, 15% to 20%, 20% to 25%, 25% to 30%, 30% to 35%, 35% to 40%, 40% to 45%, 45% to 50%, 50% to 55%, 55% to 60%, 5% to 10%, 5% to 15%, 5% to 20%, 5% to 25%, 5% to 30%, 5% to 35%, 5% to 40%, 5% to 45%, 5% to 45%, 5% to Petition 870260051474, dated 05 / 28 / 2026, page 43 / 94 29 / 58 50%, 5% to 55%, 5% to 60%, 5% to 60%, 60% to 55%, 55% to 50%, 50% to 45%, 45% to 40%, 40% to 35%, 35% to 30%, 30% to 25%, 25% to 20%, from 20% to 15%, from 15% to 10%, from 10% to 5%.

[00118] The next step e) of the method consists of determining the lowest tissue stimulation frequencies (fbx) as the point at which the tissue impedance response falls below the tolerance (NT) established in step (d). For example, three fbx are determined, a first fb1 equal to 75 kHz, a second fb2 equal to 300 kHz, a third fb3 equal to 450 kHz.

[00119] Next, step f) of the method allows determining higher tissue stimulation frequencies (ftx) as the point at which the tissue impedance response returns to the tolerance (NT) established in step (d). For example, three ftx are determined, a first ft1 equal to 100 kHz, a second ft2 equal to 350 kHz, a third ft3 equal to 495 kHz.

[00120] Alternatively, in one example the ftx is greater than the fbx.

[00121] In another example, in step (e), the frequency range between the lower tissue stimulation frequency (fbx) and the upper tissue stimulation frequency (ftx) corresponds to the central tissue frequencies.

[00122] Tissue core frequencies refer to the frequencies at which electromagnetic stimulation is attenuated due to the effects of energy absorption in the tissue, for example, the electromagnetic stimulation signal falls below 25% of a tolerance level (TL).

[00123] In addition to those mentioned above, this disclosure allows you to determine the stimulation frequency bands to focus on stimuli in said bands until the tissue impedance response returns to a tolerance level or exceeds a maximum stimulation time.

[00124] With reference to FIG. 12, the first method of tissue stimulation with a frequency-sweeping electric field comprises the following additional steps: Petition 870260051474, dated 05 / 28 / 2026, p. 44 / 94 30 / 58 in a step (g) the stimulation frequency bands based on the lower tissue stimulation frequencies (fbx) are determined, in a step (e) together with the higher tissue stimulation frequencies (ftx) are determined, in a step (f) are determined; and in a step (h) applying a magnetic field stimulus to a tissue through an array of electromagnetic transducers that receive an activation signal whose frequency varies according to the frequency stimulation bands determined in step (g) with increments or decrements in steps of a frequency delta (Δfe) during a time delta (Δte). In this way, for example, it is possible to define the frequency ranges of interest in order to perform the frequency sweep focused on said frequency bands and enable tissue stimulation more quickly than sweeping the entire frequency range between the fie and ffe fields.

[00125] In a specific example, two ftx are determined: a first ft1 equal to 100 kHz and a second ft2 equal to 350 kHz in a step (e) and two fbx are determined: a first fb equal to 75 kHz and a second fb2 equal to 300 kHz in a step (f). A first frequency stimulation band corresponds to the frequencies between fb1 and ft1 and a second frequency stimulation band corresponds to the frequencies between fb2 and ft2.

[00126] A number x of frequency stimulation bands can be determined, with x being a natural number greater than or equal to 1, that is, from a band between fb1 and ft1, continuing with a second band between fb2 and ft2 to a band between fbx and ftx.

[00127] In one example of the method, a stimulus is applied in a first stimulation frequency band starting from a frequency of fb1 equal to 75 kHz and ft1 equal to 100 kHz in steps of Δf of 100 Hz in a time delta Δt equal to 10 minutes and a second stimulus in a second Petition 870260051474, dated 05 / 28 / 2026, p. 45 / 94 31 / 58 stimulation frequency band starting from a frequency fb2 equal to 300 kHz and ft2 equal to 350 kHz in steps of Δfede 1 kHz in a time delta Δte equal to 5 minutes.

[00128] The fiee ffe, o Δfe, o NT and o Δte can be configured by a user on a computing unit and stored in a memory register.

[00129] Δfe can have a value of 0.1 Hz and 1 kHz, Δte can be between about 1 second and about 1 hour and, optionally, between about 1 minute and about 1 hour.

[00130] It can be between about 1 second and about 1 hour and, optionally, between about 1 minute and about 1 hour.

[00131] As used here, about refers to the variation of +20% to -20%.

[00132] Optionally, Δfe can be selected from about 0.1 Hz to about 1 Hz, from about 0.3 Hz to about 0.8 Hz, from about 0.5 Hz to about 0.6 Hz, from about 0.7 Hz to about 0.4 Hz, from about 0.9 Hz to about 0.2 Hz, from about 0.3 Hz to about 1 Hz, from about 0.5 Hz to about 1 Hz, from about 0.7 Hz to about 1 Hz, from about 0.9 Hz to about 1 Hz, from about 0.1 Hz to about 0.8 Hz, from about 0.1 Hz to about 0.6 Hz, from about 0.1 Hz to about 0.4 Hz, from about 0.1 Hz to about 0.2 Hz, from about 0.3 Hz to about 0.5 Hz, from about 0.5 Hz to about 0.7 Hz, from about 0.7 Hz to about 0.9 Hz, from about 0.1 Hz to about 1000 Hz, from about 100 Hz to about 900 Hz, from about 200 Hz to about 800 Hz, from about 300 Hz to about 700 Hz, from about 400 Hz to about 600 Hz, from about 500 Hz to about 500 Hz, from about 600 Hz to about 400 Hz, from about 700 Hz to about 300 Hz, from about 800 Hz to about 200 Hz, from about 900 Hz to about 100 Hz, from about 1000 Hz to about 0.1 Hz, from about 100 Hz to about 1000 Hz, from about 200 Hz to about 1000 Hz, about 300 Hz to about 1000 Hz, from about 400 Hz Petition 870260051474, dated 05 / 28 / 2026, page 46 / 94 32 / 58 to about 1000 Hz, from about 500 Hz to about 1000 Hz, from about 600 Hz to about 1000 Hz, from about 700 Hz to about 1000 Hz, from about 800 Hz to about 1000 Hz, from about 900 Hz to about 1000 Hz, from about 0.1 Hz to about 900 Hz, from about 0.1 Hz to about 800 Hz, from about 0.1 Hz to about 700 Hz, from about 0.1 Hz to about 600 Hz, from about 0.1 Hz to about 500 Hz, from about 0.1 Hz to about 400 Hz, from about 0.1 Hz to about 300 Hz, from about 0.1 Hz to about 200 Hz, from about 0.1 Hz to about 100 Hz, from about 100 Hz to about 200 Hz, from about 200 Hz to about 300 Hz, from about 300 Hz to about 400 Hz, from about 400 Hz to about 500 Hz, from about 500 Hz to about 600 Hz, from about 600 Hz to about 700 Hz, from about 700 Hz to about 800 Hz, from about 800 Hz to about 900 Hz, from about 900 Hz to about 1000 Hz.

[00133] Furthermore, an example of the method is possible, for example, where ftx are smaller than fbxe in step (a) the decreases are made in steps with a frequency delta of Δfe, during a Δte in the frequency of the activation signal.

[00134] Returning to step (a) of the method, the activation signal with the frequency varying from an initial tissue stimulation frequency (fie) to a final tissue stimulation frequency (ffe) can be applied to a transducer by means of an array of multiplexers that enable one or more electric field stimulation signals to be applied to each transducer at a given time, sequentially, out of phase with respect to the other stimulation signal or to several stimulation signals, randomly or according to a program set for each of the transducers.

[00135] With reference to FIGS. 7 and 8, it should be understood that an activation signal may consist of a modulation signal (8) and a carrier signal (9), the carrier signal (9) optionally being of a frequency order higher than that of the modulation signal (8). Petition 870260051474, dated 05 / 28 / 2026, page 47 / 94 33 / 58

[00136] In an example of the method, the modulation signal (8) has a frequency of 100 kHz, while the carrier signal (9) has a frequency less than 1 kHz.

[00137] In an example of the method, the fiee and ffe are between 0.1 Hz and 1000 kHz for the carrier signal (9) and the modulation signal (8).

[00138] Optionally, the fie frequency and ffe can be selected from the following ranges: from about 0.1 Hz to about 1 Hz, from about 0.3 Hz to about 0.8 Hz, from about 0.5 Hz to about 0.6 Hz, from about 0.7 Hz to about 0.4 Hz, from about 0.9 Hz to about 0.2 Hz, from about 0.3 Hz to about 1 Hz, from about 0.5 Hz to about 1 Hz, from about 0.7 Hz to about 1 Hz, from about 0.9 Hz to about 1 Hz, from about 0.1 Hz to about 0.8 Hz, from about 0.1 Hz to about 0.6 Hz, from about 0.1 Hz to about 0.4 Hz, from about 0.1 Hz to about 0.2 Hz, from about 0.3 Hz to about 0.5 Hz, from about 0.5 Hz to about 0.7 Hz, from about 0.7 Hz to about 0.9 Hz, from about 0.1 Hz to about 1000 Hz, from about 100 Hz to about 900 Hz, from about from 200 Hz to about 800 Hz, from about 300 Hz to about 700 Hz, from about 400 Hz to about 600 Hz, from about 500 Hz to about 500 Hz, from about 600 Hz to about 400 Hz, from about 700 Hz to about 300 Hz,from about 800 Hz to about 200 Hz, from about 900 Hz to about 100 Hz, from about 1000 Hz to about 0.1 Hz, from about 100 Hz to about 1000 Hz, from about 200 Hz to about 1000 Hz, from about 300 Hz to about 1000 Hz, from about 400 Hz to about 1000 Hz, from about 500 Hz to about 1000 Hz, from about 600 Hz to about 1000 Hz, from about 700 Hz to about 1000 Hz, from about 800 Hz to about 1000 Hz, from about 900 Hz to about 1000 Hz, from about 0.1 Hz to about 900 Hz, from about 0.1 Hz to about 800 Hz, from about 0.1 Hz to about 700 Hz, from about 0.1 Hz to about 600 Hz, from about 0.1 Hz to about 500 Hz, from about 0.1 Hz to about 400 Hz, from about 0.1 Hz to about 300 Hz, from about 0.1 Hz to about 200 Hz, from about 0.1 Hz to about 100 Hz, from about 100 Petition 870260051474, dated 05 / 28 / 2026, page. 48 / 94 34 / 58 Hz to about 200 Hz, from about 200 Hz to about 300 Hz, from about 300 Hz to about 400 Hz, from about 400 Hz to about 500 Hz, from about 500 Hz to about 600 Hz, from about 600 Hz to about 700 Hz, from about 700 Hz to about 800 Hz, from about 800 Hz to about 900 Hz, from about 900 Hz to about 1000 Hz, from about 100 kHz to about 900 kHz, from about 200 kHz to about 800 kHz, from about 300 kHz to about 700 kHz, from about 400 kHz to about 600 kHz, from about 100 kHz to about 1000 kHz, from about 200 kHz to about 1000 kHz, from about 300 kHz to about 1000 kHz, from about 400 kHz to about 1000 kHz, from about 500 kHz to about 1000 kHz, from about 600 kHz to about 1000 kHz, from about 700 kHz to about 1000 kHz, from about 800 kHz to about 1000 kHz, from about 900 kHz to about 1000 kHz, from about 0.0001 kHz to about 900 kHz, from about 0.0001 kHz to about 800 kHz, from about 0.0001 kHz to about 700 kHz, from about 0.0001 kHz to about 600 kHz, from about 0.0001 kHz to about 500 kHz, from about 0.0001 kHz to about 400 kHz, from about 0.0001 kHz to about 300 kHz, from about 0.0001 kHz to about 200 kHz, from about 0.0001 kHz to about 100 kHz, from about 100 kHz to about 200 kHz,from about 200 kHz to about 300 kHz, from about 300 kHz to about 400 kHz, from about 400 kHz to about 500 kHz, from about 500 kHz to about 600 kHz, from about 600 kHz to about 700 kHz, from about 700 kHz to about 800 kHz, from from about 800 kHz to about 900 kHz, from about 900 kHz to about 1000 kHz, from about 1 Hz to about 500 kHz, from about 1 kHz to about 500 kHz, from about 1 kHz to about 50 kHz, from about 1 Hz to about 50 kHz.

[00139] With reference to FIG. 5A, a frequency versus time graph is shown, corresponding to a modulation signal (8) for an activation signal for tissue stimulation with frequency sweep electromagnetic fields. In this example, the aforementioned modulation signal (8) increases its frequency every second by applying a Δί of Petition 870260051474, dated 05 / 28 / 2026, p. 49 / 94 35 / 58 Hz, from an initial frequency of 1 Hz to a final frequency of 5 Hz, with each frequency applied for a Δt of 1 s.

[00140] With reference to FIG. 5B, an example is shown of the activation signal by an electromagnetic stimulation applied to electromagnetic transducers in which the modulation signal (8) has a sinusoidal shape and its frequency varies from an initial frequency of 1 Hz to 5 Hz with Δf of 1 Hz every second, from 1 to 5 s. The carrier signal (9) is a pulse-type signal with a fixed period (10) of 2 ms or a fixed frequency of 500 Hz.

[00141] With reference to FIG. 5C, an example of the activation signal for an electromagnetic stimulation is shown in which the modulation signal (8) has a square shape and its frequency varies from an initial frequency of 1 Hz to 5 Hz with Δf of 1 Hz every second, from 1 to 5 s. The carrier signal (9) is a pulsed type signal with a fixed period (10) of 100 ms or a fixed frequency of 10 kHz.

[00142] With reference to FIG. 6, an example is shown of the activation signal for an electromagnetic stimulation in which the modulation signal (8) has an alternating square waveform and duty cycle variation, its frequency remains fixed and the carrier signal (9) is of the pulsed type with a fixed period (10) of 200 ps or a fixed frequency of 5 kHz.

[00143] With reference to FIG. 7, an example of the activation signal for an electromagnetic stimulation is shown where the modulation signal (8) has the form of an alternating triangular wave, its frequency is fixed and the carrier signal (9) is of the pulsed type for a fixed period (10) of 2 ps or a fixed frequency of 500 kHz.

[00144] With reference to FIG. 8, an example is shown of the activation signal for electromagnetic stimulation in which the modulation signal (8) has a segmented type function that combines a ramp Petition 870260051474, dated 05 / 28 / 2026, page 50 / 94 36 / 58 alternating and an alternating square waveform signal, the carrier signal (9) is of the pulsed type with a fixed period (10) of 2 ps or a frequency of 500 kHz.

[00145] In an example of this disclosure, the modulation signal (8) changes the duty cycle dynamically based on the tissue impedance response measured in step (b). The said duty cycle is between 0% and 100% and allows changing the electrical energy applied by transducer or transducers to the tissue (3). A duty cycle of 0% can be used, for example, to interrupt the activation of the signal for a certain period of time.

[00146] Optionally, the duty cycle of the modulation signal (8) and / or the carrier signal (9) of the activation signal can be selected from 0% to 100%, from 5% to 95%, from 10% to 90%, from 15% to 85%, from 20% to 80%, from 25% to 75%, from 30% to 70%, from 35% to 65%, from 40% to 60%, from 45% to 55%, from 10% to 100%, from 20% to 100%, from 30% to 100%, from 40% to 100%, from 50% to 100%, from 60% to 100%, from 60% to 100%, from 70% to 100%, from 80% to 100%, 90% to 100%, 0% to 90%, 0% to 80%, 0% to 70%, 0% to 70%, 0% to 60%, 0% to 50%, 0% to 40%, 0% to 30%, 0% to 20%, 0% to 10%, 10% to 20%, 20% to 30%, 30% to 40%, 40% to 50%, 50% to 60%, 60% to 70%, 70% to 80%, 80% to 90%, 90% to 100%.

[00147] With reference to FIG. 9A, an example of the tissue impedance response is shown where a tissue is stimulated with frequency-sweeping electromagnetic fields, from an initial stimulation frequency (fi) to a final stimulation frequency (ff).

[00148] Starting from fi, the tissue impedance response is measured and represented graphically, said response begins with a response value for the tissue impedance that oscillates around a maximum amplitude value (A1) as the tissue stimulation frequency increases in Δf steps at each determined Δt. Petition 870260051474, dated 05 / 28 / 2026, page 51 / 94 37 / 58

[00149] Continuing with the increments of the stimulation frequency value, the tissue impedance response value falls below the tolerance level (NT) value of the tissue impedance response. This frequency value is designated as the lower tissue stimulation frequency (fbx).

[00150] As the tissue stimulation frequency increases, the tissue impedance response continues to fall to a minimum amplitude value (A0), and said minimum value is maintained in line with increases in tissue stimulation frequency.

[00151] As the frequency value increases, the tissue impedance response value begins to rise until it reaches a tissue impedance response value that oscillates around (A1). This frequency value is designated as the upper tissue stimulation frequency (ftx) which is maintained until ff is reached.

[00152] When citing fi, ff, Δf, and Δt without the subscript “e” (fie ffe, Δfe, e Δte) or “m” (fim, ffm, Δfme Δtm), these will be understood to refer to either of the two phenomena, namely, magnetic field or electric field.

[00153] With reference to FIG. 9B, this corresponds to the approximate representation of the response of the example described in FIG. 9A, in which the tissue impedance response is smoothed through the use of averages of the impedance values ​​of the tissue impedance response, for example, using digital filters to produce said smoothing.

[00154] The digital filters selected include, among others, the group of filters consisting of FIR, Parks-McClellan, least squares, Kaiser windows, IIR filters such as Butterworth, Chebyshev, elliptic filters, among others known to a person knowledgeable in art. It is understood that the objective of smoothing the signal is to eliminate signal noise or eliminate outliers, through simple averaging or through digital filters.

[00155] With reference to FIG. 9C, an example of the response is shown. Petition 870260051474, dated 05 / 28 / 2026, page 52 / 94 38 / 58 tissue impedance, in which a tissue is stimulated with frequency-sweeping electromagnetic fields from an initial stimulation frequency (fi) to a final stimulation frequency (ff).

[00156] Starting from fie, the tissue impedance response is measured and represented graphically, said response begins with a response value for the tissue impedance that oscillates around a maximum amplitude value (A1) as the tissue stimulation frequency increases in Δf steps for each determined Δt.

[00157] Continuing with the increments of the stimulation frequency value, the tissue impedance response value falls below the tissue impedance response tolerance level (NT) value. This frequency value is designated as the lowest tissue stimulation frequency (fbx).

[00158] As the tissue stimulation frequency increases, the tissue impedance response continues to fall to a minimum amplitude value (A0), and said minimum value is maintained in alignment with the increasing tissue stimulation frequency until it reaches a final stimulation frequency (ff).

[00159] With reference to FIG. 9D, this corresponds to an example of the approximate representation of the response of the example described in FIG. 9C, in which the tissue impedance response is smoothed through the use of averages of the impedance values ​​of the tissue impedance response, for example, using digital filters to produce said smoothing.

[00160] In another example of this method, and with reference to FIG. 13, after step (h) of the method described above, the following additional steps are followed: i) establish a maximum stimulation time (tmax), which helps protect the tissue from being overstimulated; Petition 870260051474, dated 05 / 28 / 2026, page 53 / 94 39 / 58 j) measure the tissue impedance response to the stimulus in step (h); k) verify whether the tissue impedance response measured in step (j) in the stimulation frequency ranges determined in step (g) returns to the tolerance (NT) established in step (d) or exceeds the maximum stimulation time (tmax).

[00161] In an example of this method, the tmax of step (i) may be between approximately 1 hour and approximately 18 hours. When referring to tmax “approximately” it should be understood as a 5% variation from the maximum stimulation time.

[00162] In the specific example of this method, and with reference to FIG. 14, in step (k), the following checks are performed: - If the maximum stimulation time (tmax) is exceeded, terminate; - if the maximum stimulation time (tmax) is not exceeded, and the tissue impedance response measured in step (j) is below the tolerance (NT) established in step (d), repeat step (h); - if the tissue impedance response measured in step (j) in the stimulation frequency ranges determined in step (g) exceeds the tolerance (NT) established in step (d), then terminate;

[00163] With reference to FIG. 10A, an example of the tissue impedance response of a tissue stimulated from electric or magnetic fields by frequency sweeping of a wire is shown. The tissue impedance response is simultaneously measured and represented graphically. The said response begins with a tissue impedance response value that oscillates around a maximum amplitude value (A1).

[00164] As the tissue stimulation frequency continues to increase in Δf steps, it continues to oscillate around (A1) until the stimulation frequency reaches a first stimulation frequency of Petition 870260051474, dated 05 / 28 / 2026, page 54 / 94 40 / 58 lower tissue (fbi), which corresponds to a point where the tissue impedance response value falls below the NT.

[00165] As the tissue stimulation frequency continues to increase, the tissue impedance response value continues to fall to a value that oscillates around a minimum amplitude value (A0) where it is maintained until the tissue impedance response value begins to increase until it reaches (A1). This point is the first upper tissue stimulation frequency (ft1).

[00166] The frequency range between fb1 and ft1 is a first stimulation band.

[00167] Continuing the increase in tissue stimulation frequency, a second range of stimulation frequency bands is found in a frequency range between fb2 and ft2, a third range of stimulation frequency bands is found in a frequency range between fb3 and ft3, and a fourth range of frequency bands is found in a frequency range between fb4 and ft4, until the stimulation frequencies reach ff.

[00168] These stimulation bands refer to a range of central tissue frequencies that exhibit a biochemical imbalance.

[00169] Optionally, another example of the method for stimulating a tissue with electromagnetic fields, in step (e), the frequency range between the lower tissue stimulation frequency (fbx) and the upper tissue stimulation frequency (ftx) corresponds to the central frequencies of the tissue.

[00170] Alternatively, another example of the method for stimulating a tissue with electromagnetic fields, the activation signal for the electric field transducers changes with the feedback of the tissue temperature.

[00171] In another example of the method for stimulating tissue with electromagnetic fields, the biological tissue being stimulated is in an animal. Petition 870260051474, dated 05 / 28 / 2026, page 55 / 94 41 / 58

[00172] Another example of the method for stimulating tissue with electromagnetic fields, the biological tissue stimulated is an animal.

[00173] Alternatively, another example of the method for stimulating a tissue with electromagnetic fields, the electromagnetic transducers are in contact with an external surface of the tissue.

[00174] In yet another example of the method for stimulating a tissue with electromagnetic fields, the electromagnetic transducers are located at a determined distance from an external surface of the biological tissue.

[00175] In one example of the method for stimulating a tissue with electromagnetic fields, a first portion of the electromagnetic transducers is in contact with an external surface of the biological tissue and a second portion of the electromagnetic transducers is located at a determined distance from the external surface of the biological tissue.

[00176] Optionally, another example of the method for stimulating a tissue with electromagnetic fields, the electromagnetic transducers are activated according to a defined sequence.

[00177] Alternatively, another example of the method for stimulating a tissue with electromagnetic fields, the electromagnetic transducers are activated randomly.

[00178] Optionally, another example of the method for stimulating a tissue with electromagnetic fields, the activation signal is applied to each transducer at a determined time, sequentially, out of phase with respect to the other activation signal or to several stimulation signals, randomly or according to a program established for each of the transducers.

[00179] With reference to FIG. 10B, this corresponds to the approximate representation of the response to the example described in FIG. 10A, where the response Petition 870260051474, dated 05 / 28 / 2026, page 56 / 94 42 / 58 tissue impedance is smoothed, for example, by averaging the amplitude response values, using the tools described above.

[00180] Furthermore, in one example of the method, in step (a), a magnetic field stimulus is applied through the array of electromagnetic transducers that receive an activation signal, whose frequency varies from an initial tissue stimulation frequency (fm) to a final tissue stimulation frequency (ffm), increasing or decreasing in steps of a frequency delta (Δfm) during a time delta (Δtm), which makes it possible to obtain benefits of combined magnetic and electric field stimulation, such as, for example, enlarging the electric field stimulation area of ​​the tissue. In another example, in step (a), the magnetic field stimulus is orthogonal to the electric field stimulus.

[00181] Magnetic field transducers are optionally arranged so that they generate magnetic fields orthogonal to the electric fields of the electric field transducers, so that optimal tissue stimulation is provided.

[00182] However, it may be possible that the magnetic field transducers are arranged in different configurations, in which the magnetic fields generated by said magnetic field transducers are not orthogonal to the electric fields generated by the electric field transducers in the arrangement.

[00183] In another example of this disclosure, for example, the transducers of the electromagnetic transducer array (1) are magnetic field transducers and may or may not be faced by another magnetic field transducer or another electric field transducer.

[00184] To activate magnetic field transducers, the activation signal can be selected from, among other things, a direct current or alternating current signal, a pulsed signal, a train of alternating or non-alternating impulse signals, or a square wave signal with a varying duty cycle. Petition 870260051474, dated 05 / 28 / 2026, page 57 / 94 43 / 58 working, triangular wave signal, sawtooth wave signal, amplitude modulated (AM), frequency modulated (PM), phase modulated (PM), pulse position modulated (PPM), pulse width modulated (PWM) and combinations thereof.

[00185] With reference to FIGS. 1 and FIG. 15, another method of the present disclosure is tissue stimulation using magnetic fields and comprising the following steps: a') apply a magnetic field stimulus to a tissue through an array of electromagnetic transducers that receive an activation signal whose frequency varies from an initial tissue stimulation frequency (fm) to a final tissue stimulation frequency (fm) with increments or decrements in steps of a frequency delta (Δfm) during a time delta (Δtm); b') measure the tissue impedance response to magnetic field stimulation through the electric field transducers of the electromagnetic transducer array; c') establish a reference level with the tissue impedance response measured in step (b'); d') establish a tolerance (NT) to the reference level established in step (c'); e') determine the lower tissue stimulation frequencies (fbx) as the point at which the tissue impedance response falls below the tolerance (NT) established in step (d'); f') determine the upper tissue stimulation frequencies (ftx) as the point at which the tissue impedance response returns to the tolerance (NT) established in step (d'); characterized by the stimulation frequencies of the upper tissues (ftx) being higher than the stimulation frequencies of the lower tissues Petition 870260051474, dated 05 / 28 / 2026, page 58 / 94 44 / 58 lower (fbx) and “x” is a natural number greater than or equal to 1.

[00186] Magnetic transducers are placed on a volume (2) containing the tissue (3) to be stimulated. Each magnetic transducer generates a magnetic field that is applied to the tissue (3). The field generated with each transducer is controlled by an activation signal which, optionally, has an initial frequency (fim) that changes over time to a final frequency (ffm), which may be greater than, less than or equal to the initial frequency.

[00187] In one example, the intensity of the magnetic field generated by the magnetic field transducers, when activated by the activation signal, can be between 0.1 mT (milliteslas) equivalent to 1 Gauss and 200 mT (milliteslas) equivalent to 2000 Gauss and, optionally, between 40 mT (milliteslas) equivalent to 400 Gauss and 200 mT (milliteslas), equivalent to 2000 Gauss.

[00188] Optionally, the intensity generated by the magnetic field transducers is selected from the range of 1 mT to 10 mT, 10 mT to 20 mT, 20 mT to 30 mT, 30 mT to 40 mT, 30 mT to 40 mT, 40 mT to 50 mT, 50 mT to 60 mT, 60 mT to 70 mT, 70 mT to 80 mT, 80 mT to 90 mT, 90 mT to 100 mT, 90 mT to 100 mT, 100 mT to 110 mT, 100 mT to 110 mT, 110 mT to 120 mT, 120 mT to 130 mT, 130 mT to 140 mT, 140 mT to 150 mT, 150 mT to 160 mT, 160 mT to 170 mT, 160 mT to 170 mT, 160 mT to 170 mT, 170 mT to 180 mT, 180 mT to 190 mT mT, from 190 mT to 200 mT, from 1 mT to 10 mT, from 1 mT to 20 mT, from 1 mT to 30 mT, from 1 mT to 40 mT, from 1 mT to 40 mT, from 1 mT to 50 mT, from 1 mT to 50 mT, from 1 mT to 60 mT, from 1 mT to 70 mT, from 1 mT to 80 mT, from 1 mT to 90 mT, from 1 mT to 100 mT, from 1 mT to 110 mT, from 1 mT to 110 mT, from 1 mT to 120 mT, from 1 mT to 120 mT, from 1 mT to 130 mT, from 1 mT to 140 mT, from 1 mT to 150 mT, from 1 mT to 160 mT, from 1 mT to 170 mT, from 1 mT to 180 mT, from 1 mT to 180 mT,from 1 mT to 190 mT, from 1 mT to 190 mT, from 1 mT to 200 mT, from 1 mT to 200 mT, from 200 mT to 190 mT, from 190 mT to 180 mT, from 180 mT to 170, Petition 870260051474, dated 05 / 28 / 2026, page 59 / 94 45 / 58 mT, from 170 mT to 160 mT, from 160 mT to 150 mT, from 150 mT to 140 mT, from 150 mT to 140 mT, from 140 mT to 130 mT, from 130 mT to 120 mT, from 130 mT to 120 mT, from 120 mT to 110 mT, 110 mT to 100 mT, 100 mT to 90 mT, 90 mT to 80 mT, 80 mT to 70 mT, 80 mT to 70 mT, 70 mT to 60 mT, 60 mT to 50 mT, 60 mT to 50 mT mT, from 50 mT to 40 mT, from 40 mT to 30 mT, from 30 mT to 20 mT, from 20 mT to 10 mT.

[00189] The defined Δtm refers to a time period that may vary depending on the application required to use the method.

[00190] In fim and ffm, Δfm and Δtm are defined by the user and the computing unit and stored in a memory register. The applicable range values ​​for fim, ffm, Δfm and Δtm for magnetic stimulation are the same as those used for fie, ffe, Δfee and Δtm in electromagnetic stimulation mentioned earlier in this disclosure.

[00191] Optionally, the film is optionally between about 25 Hz and about 1000 kHz.

[00192] Alternatively, the end frequencies, and ffm, can be selected from the following ranges: from about 0.1 Hz to about 1 Hz, from about 0.3 Hz to about 0.8 Hz, from about 0.5 Hz to about 0.6 Hz, from about 0.7 Hz to about 0.4 Hz, from about 0.9 Hz to about 0.2 Hz, from about 0.3 Hz to about 1 Hz, from about 0.5 Hz to about 1 Hz, from about 0.7 Hz to about 1 Hz, from about 0.9 Hz to about 1 Hz, from about 0.1 Hz to about 0.8 Hz, from about 0.1 Hz to about 0.6 Hz, from about 0.1 Hz to about 0.4 Hz, from about 0.1 Hz to about 0.2 Hz, from about 0.3 Hz to about 0.5 Hz, from about 0.5 Hz to about 0.7 Hz, from about 0.7 Hz to about 0.9 Hz, from about 0.1 Hz to about 1000 Hz, from about 100 Hz to about 900 Hz, from about 200 Hz to about 800 Hz, from about 300 Hz to about 700 Hz, from about 400 Hz to about 600 Hz, from about 500 Hz to about 500 Hz, from about 600 Hz to about 400 Hz,from about 700 Hz to about 300 Hz, from about 800 Hz to about 200 Hz, from about 900 Hz to, Petition 870260051474, dated 05 / 28 / 2026, pp. 60 / 94 46 / 58 near 100 Hz, from 1000 Hz to near 0.1 Hz, from near 100 Hz to near 1000 Hz, from near 200 Hz to near 1000 Hz, from near 300 Hz to near 1000 Hz, from near 400 Hz to near 1000 Hz, from near 500 Hz to near 500 Hz to 1000 Hz, from near 600 Hz to near 1000 Hz, from near 700 Hz to near 1000 Hz, from near 800 Hz to near 1000 Hz, from near 900 Hz to near 1000 Hz, from near 0.1 Hz to near 900 Hz, from near 0.1 Hz to near 800 Hz, from near 0.1 Hz to near 700 Hz, from about 0.1 Hz to about 600 Hz, from about 0.1 Hz to about 500 Hz, from about 0.1 Hz to about 400 Hz, from about 0.1 Hz to about 300 Hz, from about 0.1 Hz to about 200 Hz, from about 0.1 Hz to about 100 Hz, from about 100 Hz to about 200 Hz, from about 200 Hz to about 300 Hz, from about 300 Hz to about 400 Hz, from about 400 Hz to about 500 Hz, from about 500 Hz to about 600 Hz, from about 600 Hz to about 700 Hz,from approximately 700 Hz to approximately 800 Hz, from approximately 800 Hz to approximately 900 Hz, from approximately 900 Hz to approximately 1000 Hz, from approximately 100 kHz to approximately 900 kHz, from approximately 200 kHz to approximately 800 kHz, from approximately 300 kHz to approximately 700 kHz, from approximately 400 kHz to approximately 600 kHz, from approximately 100 kHz to approximately 1000 kHz, from approximately 200 kHz to approximately 1000 kHz, from approximately 300 kHz to approximately 1000 kHz, from approximately 400 kHz to approximately 1000 kHz, from approximately 500 kHz to approximately 1000 kHz, from approximately 600 kHz to approximately 1000 kHz, from approximately 700 kHz to approximately 1000 kHz, from approximately 800 kHz to approximately 1000 kHz, from approximately 900 kHz to approximately 1000 kHz, from approximately 0.0001 kHz to approximately 900 kHz, from approximately 0.0001 kHz to approximately 800 kHz, from approximately 0.0001 kHz to approximately 700 kHz, from approximately 0.0001 kHz to approximately 600 kHz, from approximately 0.0001 kHz to approximately 500 kHz, from approximately 0.0001 kHz to approximately 400 kHz, from approximately 0.0001 kHz to approximately 300 kHz, from approximately 0.0001 kHz to approximately 200 kHz,from about 0.0001 kHz to about 100 kHz, from about 100 kHz to about 200 kHz, from about 200 kHz to about 300 kHz, from about 300 kHz to about 400 kHz, from about 400 kHz to about, Petition 870260051474, dated 05 / 28 / 2026, page. 61 / 94 47 / 58 500 kHz, from about 500 kHz to about 600 kHz, from about 600 kHz to about 700 kHz, from about 700 kHz to about 800 kHz, from about 800 kHz to about 900 kHz, from about 900 kHz to about 1000 kHz, from about 1 Hz to about 500 kHz, from about 1 kHz to about 500 kHz, from about 1 kHz to about 50 kHz, from about 1 Hz to about 50 kHz.

[00193] In an example of disclosure, similarly to the activation signal used for electric field stimulation, the activation signal used for magnetic fields can also be configured by a carrier signal (9) and a modulation signal (8). For example, the fim and ffm are between 0.1 Hz and 500 kHz for both the carrier signal (9) and the modulation signal (8), with the carrier signal (9) optionally having a frequency order higher than that of the modulation signal (8).

[00194] In one example of the method, in one example, in step (d'), the NT can be between 5% and 60% and, optionally, between 25% and 50%. The applicable range values ​​for NT for magnetic stimulation are the same as those used for NT in electromagnetic stimulation mentioned earlier in this document.

[00195] In another example of this method, in step (e'), the frequency range between the lower tissue stimulation frequency (fbx) and the upper tissue stimulation frequency (ftx) corresponds to the central frequencies of the tissue.

[00196] In addition, the control unit can be programmed to select the initial tissue stimulation frequency (fm), the final tissue stimulation frequency (ffm) and the frequency delta (Δfm), and optionally, these characteristics are defined by a user and stored in a memory for application of the method.

[00197] A pulse function can be used to activate the magnetic field, where fm and fm are between 0.1 Hz and 1000 kHz, Δfm has a range between 0.1 Hz and 1 kHz, Δtm is between 1 second and 1 hour and, Petition 870260051474, dated 05 / 28 / 2026, pp. 62 / 94 48 / 58 optionally, between 1 minute and 1 hour.

[00198] Furthermore, it may be possible to use an activation signal for square wave frequency sweep magnetic stimulation at a frequency of 1 Hz to 50 kHz. For frequencies from 1 Hz to 5 kHz, apply a duty cycle variation between 0.4% and 5% for a maximum magnetic field intensity of 200 mT (milliteslas) equivalent to 2000 Gauss. Subsequently, for frequencies from 5 kHz to 50 kHz, apply a duty cycle variation between 5% and 25% for a maximum magnetic field intensity of 40 mT (milliteslas) equivalent to 400 Gauss. This frequency sweep is applied for a tmax of 1 hour and can be repeated daily for 6 days.

[00199] In addition to what is described above, and with reference to FIG. 15, in another example of the method, after step (f'), the following steps are completed: g') determine stimulation frequency bands based on the lower tissue stimulation frequencies (fbx) determined in step (e') together with the upper tissue stimulation frequencies (ftx) determined in step (f'); h') apply a magnetic field stimulus to a tissue through an array of electromagnetic transducers that receive an activation signal, whose frequency varies according to the stimulation frequency ranges determined in step (g') with increments or decrements in the steps of a frequency delta (Δfm) during a time delta (Δtm);

[00200] In another example of this method, and with reference to FIG. 16, after step (h') of the method described above, the following additional steps are followed: i') establish a maximum stimulation time (tmax), which makes it possible to protect the tissue from being overstimulated; b') measure the tissue impedance response to the stimulus of Petition 870260051474, dated 05 / 28 / 2026, pp. 63 / 94 49 / 58 stage; (h') through electric field transducers of the electromagnetic transducer array; k') verify if the tissue impedance response measured in step (j') in the stimulation frequency ranges determined in step (g'), returns to the tolerance (NT) established in step (d') or exceeds the maximum stimulation time (tmax).

[00201] In an example of this method, tmax in a step (i') can be between about 1 hour and about 18 hours. When referring to tmax “about” it should be understood as a 5% variation from the maximum stimulation time.

[00202] In a specific example of this method, in step (k'), the following checks are performed: - If the maximum stimulation time (tmax) is exceeded, terminate; - if the maximum stimulation time (tmax) is not exceeded, and the tissue impedance response measured in step (j') is below the tolerance (NT) established in step (d'), repeating step (h'); - if the tissue impedance response measured in step (j') in the stimulation frequency ranges determined in step (g') exceeds the tolerance (NT) established in step (d'), then terminate;

[00203] In addition to what has been described above, tissue stimulation can also be applied using spatial scanning, as described in the Colombian application serial number NC2018 / 0001282, filed on February 7, 2018.

[00204] In another example of the method, the activation signal follows a defined pattern, which follows these steps: A) Define an index for each magnetic field transducer in the transducer array; Petition 870260051474, dated 05 / 28 / 2026, pp. 64 / 94 50 / 58 B) Select a localized index for a transducer using the computing unit; C) activate the magnetic field transducer that corresponds to the selected index and stimulate the tissue using an initial tissue stimulation frequency (fi) to a final tissue stimulation frequency (ffm) in increments of a frequency delta (Δfm) during a given time delta (Δtm); D) increasing the index value and repeating step C) until all assigned indices have been used.

[00205] Alternatively, in a specific example of the method, in step D), the index value changes randomly and returns to step C).

[00206] Thus, the transducers can be activated randomly, and it is also possible to activate them in defined sequences, which will depend on the stimulation of the target tissue.

[00207] Optionally, in another example of the method of stimulating a tissue with magnetic fields, in step (e'), the frequency range between the lower tissue stimulation frequency (fbx) and the upper tissue stimulation frequency (ftx) corresponds to the central frequencies of the tissue.

[00208] Alternatively, in another example of the method for stimulating a tissue with magnetic fields, the activation signal for the electric field transducers changes with feedback from the tissue temperature.

[00209] In another example of the method for stimulating tissue with magnetic fields, the biological tissue being stimulated is in an animal.

[00210] In another example of the method for stimulating tissue with magnetic fields, the biological tissue stimulated is an animal.

[00211] Alternatively, another example of the method for stimulating a tissue with magnetic fields, the electromagnetic transducers are in contact with an external surface of the tissue. Petition 870260051474, dated 05 / 28 / 2026, pp. 65 / 94 51 / 58

[00212] In yet another example of the method for stimulating a tissue with magnetic fields, the electromagnetic transducers are located at a determined distance from an external surface of the biological tissue.

[00213] In a different example of the method for stimulating a tissue with magnetic fields, a first portion of the electromagnetic transducers is in contact with an external surface of the biological tissue and a second portion of the electromagnetic transducers is located at a determined distance from the external surface of the biological tissue.

[00214] Optionally, in another example of the method for stimulating a tissue with magnetic fields, the electromagnetic transducers are activated according to a defined sequence.

[00215] Alternatively, in another example of the method for stimulating a tissue with electromagnetic fields, the electromagnetic transducers are activated randomly.

[00216] Optionally, in another example of the method for stimulating a tissue with magnetic fields, the activation signal is applied to each transducer at a determined time, sequentially, out of phase with respect to the other activation signal or to several stimulation signals, randomly or according to a program established for each of the transducers.

[00217] In another example of this disclosure, it is possible to stimulate a tissue (3) with an array of electromagnetic transducers (1) with electric field transducers and magnetic field transducers by applying a combination of electrical stimulation signals and magnetic stimulation signals that combine their characteristics on the basis of feedback of the intensity of the magnetic field applied to the tissue, feedback of the tissue impedance or a combination of both.

[00218] A third method for tissue stimulation is a method Petition 870260051474, dated 05 / 28 / 2026, pp. 66 / 94 52 / 58 to stimulate a tissue with electric and magnetic fields, the method comprising: a *) Apply an electric field and a magnetic field stimulus to a tissue through an array of electromagnetic transducers that receive an activation signal, whose parameters vary over time; b *) measure the tissue impedance response to the stimulus from step (a*) by arranging the electromagnetic transducers and store said measurement in the memory register of a computing unit; c *) change the activation signal parameters according to the tissue impedance response measured in step (b *) by the computing unit and returning to step (a*); characterized by activation signal parameters being modulation, phase, frequency, amplitude, duration, duty cycle and shape.

[00219] In addition, it is possible to measure the temperature on the tissue to determine when the tissue being stimulated is affected and, based on a temperature level, the computing unit makes the decision to stop the stimulus to avoid tissue damage.

[00220] In other words, it is possible that the activation signal of magnetic transducers follows a pattern that changes with feedback from tissue temperature, and this also applies to the activation signal of electric field transducers.

[00221] It may be possible to combine tissue stimulation with the frequency-sweep electric field method and tissue stimulation with the frequency-sweep magnetic field method. In one example, the electromagnetic transducer arrangement (1) comprises magnetic field transducers and electric field transducers superimposed on each other, arranged orthogonally to each other, with their active faces in the direction of the tissue (3), in order to apply a combination stimulation by Petition 870260051474, dated 05 / 28 / 2026, pp. 67 / 94 53 / 58 electric and magnetic fields following an activation pattern. Example of the disclosure method applied to an individual.

[00222] In an example from this disclosure, tissue stimulation is performed on an individual with the following initial diagnosis of poorly differentiated malignant neoplasm shown in FIG. 11A. Immunohistochemical markers show the following results: - Cytokeratin 5 / 6: positive in tumor cells; - P 63: positive in tumor cells; - High molecular weight cytokeratin: positive in tumor cells; - SOX10: negative in tumor cells; and, - Ber-EP4: negative in tumor cells.

[00223] The tissue was stimulated with a device for stimulating a tissue with electromagnetic fields that implements the method for stimulating a tissue of the present disclosure. The device used the following arrangement of electromagnetic transducers: - 2 pairs of orthogonally arranged electric field transducers (2 x 3.5 inch disposable electrotherapy electrodes manufactured by Compass Health Brands Corp., for use as a disposable conductive adhesive interface between the patient's skin and the electrical stimulator), and - 1 pair of magnetic field transducers arranged orthogonally to the two pairs of electric field transducers.

[00224] According to the arrangement described, a magnetic field stimulus and an electric field stimulus are applied orthogonally to each other.

[00225] The device's computing unit generates the activation signal according to a program established for each magnetic transducer. Specifically, the pair of magnetic field transducers was Petition 870260051474, dated 05 / 28 / 2026, pp. 68 / 94 A 54 / 58 transistor connected to the first and second channels of the device received an activation signal with an amplitude of 72 Vpp (peak-to-peak voltage) and applied a frequency sweep ranging from an initial end tissue stimulation frequency of 1 Hz to a final tissue stimulation frequency of 50 kHz. From this frequency, it sweeps two stimulation frequency bands as determined by the computing unit; the first stimulation frequency band corresponded to a frequency range from a first lower tissue stimulation frequency fb1 equal to 1 Hz to a first upper tissue stimulation frequency ft1 equal to 5 kHz, and the second stimulation frequency band corresponded to a frequency range from a second lower tissue stimulation frequency fb2 equal to 5 kHz to a second upper tissue stimulation frequency ft2 equal to 50 kHz.

[00226] The two pairs of electric field transducers were connected respectively to a third, fourth, fifth, and sixth channel of the device. Each pair received an activation signal with an amplitude of 72 Vpp (peak-to-peak voltage). The carrier signal was an alternating pulse train with a fixed frequency of 150 kHz modulated in PWM with a 15% duty cycle; the modulation signal was a variable frequency triangular waveform. This activation signal was applied in a frequency sweep ranging from an initial tissue stimulation frequency fie equal to 1 kHz to a final tissue stimulation frequency ffe equal to 500 kHz.Based on the aforementioned frequency sweep, three stimulation frequency bands were determined by the computing unit: the first stimulation frequency band corresponded to a frequency range from a first lower tissue stimulation frequency fb1 equal to 1 kHz to a first upper tissue stimulation frequency ft1 equal to 50 kHz; the second stimulation frequency band corresponded to a frequency range of a second. Petition 870260051474, dated 05 / 28 / 2026, p. 69 / 94 55 / 58 lower tissue stimulation frequency fb2 equal to 150 kHz to a second upper tissue stimulation frequency ft2 equal to 250 kHz; and the third stimulation frequency range corresponded to a frequency range of a third lower tissue stimulation frequency fb3 equal to 320 kHz to a third upper tissue stimulation frequency ft3 equal to 420 kHz.

[00227] The average measured power delivered to the tissue in the example above ranged between 0.2 W and 0.5 W. The electrode temperature never exceeded 40 °C.

[00228] After determining the stimulation frequency bands, the device for stimulating tissue with electromagnetic fields applied activation signals in each of these stimulation frequency bands for one hour per day, for a total of 6 days. In all cases, the frequency delta steps (Δf) were 500 Hz and the time delta steps (Δt) were 1 second.

[00229] With reference to FIG. 11B, a photograph is shown of the same individual exhibiting a marked reduction in malignant tumor mass after the delivery schedule mentioned above. Furthermore, the laboratory report after stimulation was negative for metastases.

[00230] With reference to FIG. 11C, the graph shows a tissue impedance signal (34) that corresponds to the tissue impedance response through channels 5 and 6 to the third stimulation frequency band mentioned above (fb3 = 320 kHz; ft3 = 420 kHz), and correlates in time with an intermediate step in the individual's response between FIG. 11A and FIG. 11B. As the graph shows, the tissue impedance signal (34) begins to show a drop at about 140 seconds, when the applied tissue stimulation frequency was about 320 kHz. This drop, or valley, lasted until 340 seconds, when the applied tissue stimulation frequency reached about 420 kHz. Petition 870260051474, dated 05 / 28 / 2026, pp. 70 / 94 56 / 58

[00231] A trend was observed in which the response valley to The previous tissue impedance was not linked to specific frequencies throughout each application. Instead, small changes in the frequency of the tissue impedance response were observed, typically showing a variation of + / - 20%. Over the 6 days of delivery, the tissue impedance valleys tended to disappear.

[00232] FIG. 11D shows a tissue impedance response signal smoothed (35) and was achieved by averaging the tissue impedance response signal (34). DEFINITIONS AND ACRONYMS AM Amplitude Modulation AMOLED Active Matrix Organic Light Emitting Diode ASIC Application-Specific Integrated Circuits CPLD Programmable Logic Device DSC Digital Signal Controllers EEG Electroencephalogram EMF Electromagnetic Fields. fbx Lower tissue stimulation frequencies: refers to the lowest frequency of a stimulation band, the frequency sweep stimulation. ffe Final tissue stimulation frequency: refers to the final frequency of frequency sweep stimulation with electric fields. ffm Final tissue stimulation frequency: refers to the final frequency of sweep stimulation with magnetic fields. fie Initial tissue stimulation frequency: refers to the initial frequency of sweep stimulation. Petition 870260051474, dated 05 / 28 / 2026, pp. 71 / 94 57 / 58 Frequency with electric fields. f. 1 im Initial tissue stimulation frequency: refers to the initial frequency of frequency sweep stimulation with magnetic fields. FM Frequency Modulation FPGA Field Programmable Gate Arrays ftx Upper tissue stimulation frequency: refers to the final frequency of frequency sweep stimulation. HID Human Interface Device LCD Liquid Crystal Display LED Light Emitting Diode MFG Magnetic Field Generator. NT Tolerance is a percentage value of the reference level value, which the method determines an fbx or ftx based on whether or not the tissue impedance response exceeds the said percentage value. OLED Organic Light Emitting Diode PEMF Pulsed Electromagnetic Fields PM Phase Modulation PPM Pulse Position Modulation PSoC Programmable Systems on Chip PWM Pulse Width Modulation QD Quantum Display SoC Systems on Chip SPMF Programmed Magnetic Fields.f Frequency delta: refers to the increases or decreases in steps of the stimulation frequency. Petition 870260051474, dated 05 / 28 / 2026, pp. 72 / 94 58 / 58 Frequency sweep with electric fields. Δfm Frequency Delta: refers to the increments or decrements in steps of the frequency of the frequency sweep stimulation with magnetic fields. Δte Time Delta: refers to the duration or time period of the frequency sweep stimulation with electric fields. Δtm Time Delta: refers to the duration or time period of the frequency sweep stimulation with magnetic fields. Reference Level Refers to a value established by a user or computing unit to scale, adjust, or fix the tissue impedance response within a range of amplitude values ​​to be presented or analyzed for a user. Frequency bands refer to a range of frequencies in which the response The amplitude of tissue impedance stimulation falls below the tolerance.

[00233] This disclosure is not limited to the illustrated examples described, since, as will be obvious to those skilled in the art, There are possible variations and modifications that do not deviate from the spirit of the publication, which is defined only by the following claims. Petition 870260051474, dated 05 / 28 / 2026, pp. 73 / 94

Claims

1 / 5 CLAIMS 1. Device for tissue stimulation using electromagnetic fields, the device characterized in that it comprises: a computing unit; an external power source connected to the computing unit; a decoupling circuit connected to the external power source and the computing unit; and an arrangement of electromagnetic transducers connected to the computing unit and the decoupling circuit, the arrangement being functionally arranged on the tissue;in which the computing unit implements a method for tissue stimulation with electromagnetic fields, the method comprises the steps of: a) applying an electric field stimulus to a tissue through an array of electromagnetic transducers that receive an activation signal, whose frequency varies from an initial tissue stimulation frequency (fie) to a final tissue stimulation frequency (ffe) with increments or decrements in steps of a frequency delta (Δfe) over a time delta (Δte); b) measuring a tissue impedance response to the electric field stimulus in step (a); c) establishing a reference level with the tissue impedance response measured in step (b); d) establishing a tolerance (NT) to the reference level established in step (c);e) determination of lower tissue stimulation frequencies (fbx) as a point where the tissue impedance response falls below the tolerance (NT) established in step (d); and f) determination of upper tissue stimulation frequencies (ftx) as a point where the tissue impedance response returns to the tolerance (NT) established in step (d); wherein the upper tissue stimulation frequencies (ftx) are greater than the lower tissue stimulation frequencies (fbx) and “x” Petition 870260051474, dated 05 / 28 / 2026, page 74 / 94 2 / 5 is a natural number greater than or equal to 1.; 2. Device according to claim 1, characterized in that the tolerance (NT) is about 25% and about 50%.

3. Device according to claim 1, characterized in that the initial tissue stimulation frequency (fie) and the final tissue stimulation frequency (ffe) are about 0.1Hz and about 1000kHz, respectively.

4. Device according to claim 1, characterized in that the frequency delta (Δfe) has a value of approximately 0.1Hz and approximately 1kHz.

5. Device according to claim 1, characterized in that the time delta (Δte) is between approximately 1 second and approximately 1 hour.

6. Device according to claim 1, characterized in that, after step (f), the following steps are completed: g) determination of the stimulation frequency bands based on the lower tissue stimulation frequencies (fbx) determined in step (e) together with the upper tissue stimulation frequencies (ftx) determined in step (f); eh) application of an electric field stimulus to a tissue through an array of electromagnetic transducers that receive an activation signal whose frequency varies with the stimulation frequency bands determined in step (g) with increments or decrements in steps of a frequency delta (Δfe) over a time delta (Δte); i) establishment of a maximum stimulation time (tmax); j) measurement of the tissue impedance response in the stimulation step (h);ek) verification that the tissue impedance response measured in step (j) in the stimulus frequency bands determined in step (g) returns to the tolerance (NT) established in step (d) or exceeds the maximum stimulation time (tmax).; 7. Device according to claim 6, characterized Petition 870260051474, dated 05 / 28 / 2026, page 75 / 94 3 / 5 by step (k) comprising the following checks: if the maximum stimulation time (tmax) is exceeded, then terminating; if the maximum stimulation time (tmax) is not exceeded, and the tissue impedance response measured in step (j) is at a value lower than the tolerance (NT) established in step (d), repeating step (h); and if the tissue impedance response measured in step (j) in the stimulation frequency bands determined in step (g) exceeds the tolerance (NT) established in step (d), then terminating.

8. Device for tissue stimulation with electromagnetic fields, the device characterized in that it comprises: a computing unit; an external power source connected to the computing unit; a decoupling circuit connected to the external power source and the computing unit; and an arrangement of electromagnetic transducers connected to the computing unit and the decoupling circuit, the arrangement being functionally arranged on the tissue;in which the computing unit implements a method for stimulating a tissue with electromagnetic fields, the method comprises the steps of: a') applying a magnetic field stimulus to a tissue through an array of electromagnetic transducers that receive an activation signal, whose frequency varies from an initial tissue stimulation frequency (fm) to a final tissue stimulation frequency (fm) with increments or decrements in steps of a frequency delta (Δfm) over a time delta (Δtm); b') measuring a tissue impedance response to the magnetic field stimulus through electric field transducers of the electromagnetic transducer array; c') Petition 870260051474, dated 05 / 28 / 2026, page 76 / 94 4 / 5 establishing a reference level with the tissue impedance response measured in step (b'); d') establishing the tolerance (NT) at the reference level established in step (c');e') determination of the lower tissue stimulation frequencies (fbx) as the point where the tissue impedance response falls below the tolerance (NT) established in step (d'); and f') determination of the upper tissue stimulation frequencies (ftx) as the point where the tissue impedance response returns to the tolerance (NT) established in step (d'); wherein the upper tissue stimulation frequencies (ftx) are greater than the lower tissue stimulation frequencies (fbx) and “x” is a natural number greater than or equal to 1.

9. Device according to claim 8, characterized in that after step (f'), the following steps are completed: g') determination of the stimulation frequency bands based on the lower tissue stimulation frequencies (fbx) determined in step (e') together with the upper tissue stimulation frequencies (ftx) determined in step (f'); h') application of a magnetic field stimulus to a tissue through an array of electromagnetic transducers that receive an activation signal, whose frequency varies through the stimulation frequency bands determined in step (g') with increments or decrements in steps of a frequency delta (Δfm) over a time delta (Δtm); i') establishment of a maximum stimulation time (tmax); and j') measurement of the tissue impedance response to the stimulus of step (h') through electric field transducers of the electromagnetic transducer array;and k') verification that the tissue impedance response measured in step (j') in the stimulation frequency bands determined Petition 870260051474, dated 05 / 28 / 2026, page 77 / 94 5 / 5 in step (g') return to the tolerance (NT) established in step (d') or exceed the maximum stimulation time (tmax).; 10. Device according to claim 1, characterized in that the computing unit is a special-purpose computing unit comprising a central processing unit (CPU) connected to oscillators, a first oscillator OSC 1, a second oscillator OSC 2, and an oscillator OSC n, wherein each oscillator has an activation output signal; wherein n is a natural number greater than or equal to zero.

11. Device according to claim 10, characterized in that the central processing unit is also connected to a peripheral device selected from among others, such as storage devices like a memory unit, a database and a hard drive, input devices like a keyboard, a camera, a touch screen, and a scanner, and output devices like a monitor and a printer. Petition 870260051474, dated 05 / 28 / 2026, pp. 78 / 94

Images