Detection method and application of molecular markers for single nucleotide polymorphism of foxo1 gene in Qinchuan cattle

A technology of single nucleotide polymorphism and detection method, applied in the field of single nucleotide polymorphism of Qinchuan cattle FoxO1 gene and its detection, can solve problems such as lack of research, improve selection efficiency, speed up breeding progress, Accurately estimated effect

CN103233001BInactive Publication Date: 2016-11-02NORTHWEST A & F UNIV
2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2016-11-02
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses cattle FoxO1 gene mononucleotide polymorphism and a detection method thereof. The gene mononucleotide polymorphism comprises that: with a Qinchuan cattle whole genome DNA comprising the FoxO1 gene as a template, and with a primer pair P as primers, the Qinchuan cattle FoxO1 gene is subjected to PCR amplification; a restriction endonuclease HhaI is used for digesting the PCR amplification product, and the digested segment is subjected to agarose gel electrophoresis; and according to the electrophoresis result, base polymorphism on the 178132 site of the Qinchuan cattle FoxO1 gene is identified. The FoxO1 gene is important for animal muscle fat traits and growth traits. With the method, the molecular genetic marker closely related to Qinchuan cattle growth traits is screened and detected on a DNA level. The method is used in Qinchuan cattle assisted selection and molecular breeding, and assists in accelerating Qinchuan cattle breeding speed.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of molecular genetics, and relates to a single nucleotide polymorphism (SNP) of a functional gene of Qinchuan cattle as a molecular genetic marker, in particular to a single nucleotide polymorphism of the FoxO1 gene of Qinchuan cattle and its detection method. Background technique

[0002] In beef cattle breeding, people expect to achieve the purpose of early selection and improve the accuracy of breeding values ​​through the selection of DNA markers that are closely related to growth traits and closely linked to quantitative traits, so as to obtain greater heredity in livestock and poultry breeding. progress.

[0003] Molecular breeding, that is, Molecular Mark-Assist Selection (MAS), is a technology that uses DNA molecular markers to select genetic resources or breeding materials, and to improve the comprehensive traits of livestock and poultry. It uses modern molecular The method of combining biology and traditio...

Examples

Embodiment Construction

[0035] In the present invention, primers are designed to amplify the 405bp fragment of the third exon of FoxO1 gene based on the conservative sequence of FoxO1 gene, and the Qinchuan cattle genome is used as a template to carry out PCR amplification, and the amplified product is sequenced to search for the single nucleotide of the amplified fragment Polymorphism: Perform trait correlation analysis for the discovered single nucleotide polymorphism, and provide its detection method, making the nucleotide polymorphism of the FoxO1 gene a molecular genetic marker that can be quickly and conveniently detected, in order to speed up the establishment of Qinchuan cattle population with high-quality economic traits provides a basis.

[0036] a. Detection of FoxO1 gene polymorphism in Qinchuan cattle

[0037] 1. Collection and processing of Qinchuan cattle blood samples

[0038] Take Qinchuan cattle blood sample 10mL, add 0.5mol / L EDTA 500μL anticoagulant, slowly invert 3 times, put it...