Method for selecting molecular marker for goat yeaning traits

A molecular marker and goat technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of expensive detection, cumbersome operation, and non-existence, so as to speed up breeding progress, improve selection efficiency, and accurately estimate Effect

CN101899526BInactive Publication Date: 2012-08-29NORTHWEST A & F UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2012-08-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for selecting a molecular marker for goat yeaning traits, which comprises the following steps of: taking a goat genome DNA sequence as a template, amplifying KITL gene introns 1 and 6 by using primers P1 and P2 under a PCR condition in the presence of TaqDNA polymerase, buffer environment, Mg2+ and dNTPs respectively, and judging the size of a destination fragmentaccording to an agarose gel electrophoresis result; digesting the PCR amplification product of the primer P1 by using restriction enzyme CviAII, and then detecting the enzyme digested amplification fragment by using polyacrylamide gel electrophoresis, wherein the amplification product of the primer P1 has mutation of two basic groups; detecting the PCR amplification product of the primer P2 by adopting the polyacrylamide gel electrophoresis, wherein the amplification product of the primer P2 has mutation of one basic group; and then performing gene analysis and gene frequency analysis on the amplification products of the primers P1 and P2, and performing association analysis between the amplification products and the yeaning numbers of Guanzhong dairy goats, western Saanen dairy goats andBoer goats, wherein the analysis results show that an SNPs site of the KITL gene, detected by the primers P1 and P2, can be taken as the molecular marker for goat yeaning trait selection.
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Description

technical field

[0001] The invention belongs to the field of molecular genetics, and specifically relates to a molecular marker selection method for lambing traits of goats. The method adopts detection of mutation polymorphisms of goat stem cell factor (Kit ligand, KITL) gene intron 1 and 6 bases, and Analysis of its effect on litter size showed that the SNPs of KITL gene detected by primers P1 and P2 could be used as molecular markers for lambing traits selection in goats. Background technique

[0002] With the rapid development of molecular biology technology, especially the advent of Polymerase Chain Reaction (Polymerase Chain Reaction) technology and the improvement of electrophoresis technology, various molecular genetic marker technologies have emerged. Breeding of varieties provides new ways and methods. In particular, the combination of molecular marker technology and conventional breeding technology has given birth to a brand-new selection method - marker-assisted ...

Examples

Embodiment Construction

[0037] A. PCR amplification of intron 1 and 6 of KITL gene and detection of its polymorphism

[0038] 1. Collection and processing of goat blood samples

[0039] Take 5 mL of goat blood sample, add 0.2 μL of ACD (2.4 g of citric acid; 6.6 g of trisodium citrate; 7.35 g of glucose; dilute to 50 mL, autoclave) for anticoagulation, slowly invert 3 times and put it in an ice box, Store at -80°C for later use.

[0040] In this example, a total of 681 ewe blood samples from 3 goat populations were used, specifically: 290 blood samples of Xinong Saneng sheep, collected from Qianyang Saneng sheep breeding farm in Shaanxi Province; 193 blood samples of Guanzhong dairy goats, collected from Shaanxi Zhouzhi Green New Century Biological Co., Ltd.; 198 Boer goat blood samples were collected from the Boer goat breeding farm in Linyou County, Shaanxi.

[0041] 2. Extraction and purification of genomic DNA from blood samples

[0042] 1) Thaw the frozen blood sample at room temperatur...