A method for detecting single nucleotide polymorphism of sheep kitlg gene and its application

A single nucleotide polymorphism, sheep technology, applied in the field of molecular genetics, can solve problems such as few reports on sheep research, and achieve the effects of accelerating breeding progress, low cost and simple method

CN106498078BActive Publication Date: 2019-05-21天津奥群羊业研究院有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2019-05-21

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Abstract

The invention discloses a method for detecting single nucleotide polymorphism of a sheep KITLG gene and application of the method. The method comprises the following steps: carrying out PCR amplification on fragments of the sheep KITLG gene in a manner of taking a whole-genome DNA of sheep to be detected as a template and taking a primer pair P as primers; digesting a PCR amplification product with restriction endonuclease SspI, and then, carrying out agarose gel electrophoresis on the amplified fragments subjected to digestion; identifying the polymorphism of a 47569-th base of the sheep KITLG gene according to electrophoresis results. The mutant site of the base is closely associated with reproduction traits, i.e., litter size of the sheep, so that the method is a method for detecting molecular genetic markers, which are closely related to the reproduction traits of the sheep, from a DNA level, and can be applied to the assisted selection and molecular breeding of the sheep, and thus, the good-variety breeding speed of the sheep is accelerated.
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Description

technical field

[0001] The invention belongs to the field of molecular genetics, and relates to using the single nucleotide polymorphism (SNP) of sheep's functional gene as a molecular genetic marker, in particular to the single nucleotide polymorphism of sheep KITLG gene and a detection method thereof. Background technique

[0002] In animal breeding, people expect to achieve early selection and improve the accuracy of breeding values ​​by selecting DNA markers that are closely related to growth, reproduction and other traits and are closely linked to quantitative traits, so as to obtain better results in livestock and poultry breeding. Great genetic advances.

[0003] Molecular breeding, that is, Molecular Mark-Assist Selection (MAS), is a technology that uses DNA molecular markers to select genetic resources or breeding materials, and to improve the comprehensive traits of livestock and poultry. It uses modern molecular The method of combining biology and traditional gen...

Examples

Embodiment Construction

[0031] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, and the described embodiments are explanations of the present invention, rather than limitations.

[0032]The invention uses the conservative sequence of KITLG gene to design primers to amplify the 468bp fragment of the fifth intron of KITLG gene, and uses the sheep genome as a template to carry out PCR amplification, and the amplified product is sequenced to search for the single nucleotide polymorphism of the amplified fragment ; Conduct trait correlation analysis for the discovered single nucleotide polymorphism, and provide its detection method, making the nucleotide polymorphism of the KITLG gene a molecular genetic marker that can be quickly and conveniently detected, in order to speed up the establishment of a high-quality Provide evidence for breeding sheep populations.

[0033] a. Detection of sheep KITLG gene polymorphism

[0034] 1. ...