Single nucleotide polymorphism of ox TAS1R2 gene and detecting method thereof

A single nucleotide polymorphism and detection method technology, applied in the field of single nucleotide polymorphism and its detection of cattle TAS1R2 gene, can solve problems such as lack of research, improve selection efficiency, accurately estimate, and accelerate breeding progress Effect

CN101962683AInactive Publication Date: 2011-02-02XUZHOU NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2011-02-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a single nucleotide polymorphism of an ox TAS1R2 gene and a detecting method thereof. The detecting method of the single nucleotide polymorphism of the gene comprises the following steps of: with an ox total genome DNA to be detected, which comprises a TAS1R2 gene, as a template and with a primer pair P as a primer, carrying out PCR (Polymerase Chain Reaction) amplification on the ox TAS1R2 gene; after denaturing the product, carrying out polyacrylamide gel electrophoresis on a denatured amplification fragment; and identifying the 288bp base polymorphism of the ox TAS1R2 gene according to an electrophoresis result. The method is used for screening and detecting molecular genetic markers which are closely relevant to ox meat producing characters at a DNA level to beused for the assisted selection and molecular breeding of oxen and improving the breeding speed of fine breeds of the oxen.
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Description

technical field

[0001] The invention belongs to the field of molecular genetics, and relates to using the single nucleotide polymorphism (SNP) of the functional gene of cattle as a molecular genetic marker, in particular to a single nucleotide polymorphism of the TAS1R2 gene of cattle and a detection method thereof. Background technique

[0002] In recent years, my country's beef cattle production has developed rapidly, but the production level is low, and the average meat production rate is not high. One of the important reasons is that my country has not yet cultivated a specialized beef cattle breed. In order to vigorously develop beef cattle production in our country, we must cultivate our own beef cattle breeds in addition to using foreign excellent beef cattle breeds to improve local cattle in our country.

[0003] As an important part of the treasure house of cattle breed resources in the world, my country's yellow cattle breed resources are very rich, with strong ada...

Examples

Embodiment Construction

[0032] The present invention uses the conservative sequence of the TAS1R2 gene to design primers to amplify the 215bp fragment of the first exon of the TAS1R2 gene, respectively use the genomes of three cattle breeds as templates to perform PCR amplification, purify the PCR products, and search for them after SSCP analysis and sequencing. The single nucleotide polymorphism of the amplified fragment; the trait association analysis is carried out for the found single nucleotide polymorphism, and its detection method is provided, making the nucleotide polymorphism of the TAS1R2 gene a fast and convenient method. The detected molecular genetic markers provide a basis for accelerating the establishment of cattle populations with high-quality economic traits.

[0033] a. Amplification of partial DNA sequence of cattle TAS1R2 gene and detection of its polymorphism

[0034] 1. Collection and processing of cattle blood samples

[0035] Take 10 mL of cattle blood sample, add 500 μL of ...