A biological composite Trichoderma inhibitor and preparation method thereof

Abstract

The present invention belongs to the field of microbial technology for preventing and controlling edible fungi Trichoderma contamination, and specifically relates to a biological composite Trichoderma inhibitor and a preparation method thereof. The purpose of the present invention is to solve the problems of low control effect, large residual amount and high cost of the existing Trichoderma prevention and control agents. The technical scheme of the present invention is: a biological composite Trichoderma inhibitor, which is prepared by fermenting Bacillus subtilis ACCC11088 and Jingyang Streptomyces ACCC40041 by single strain fermentation culture to prepare fermentation liquid, and then the two fermentation liquids are mixed uniformly in a weight ratio of 1 to 5: 5 to 1 to prepare the biological composite Trichoderma inhibitor; or the filtrate remaining after filtering the bacterial bodies of the two fermentation liquids is mixed uniformly in a weight ratio of 1 to 5: 5 to 1 to prepare the biological composite Trichoderma inhibitor. The present invention has the advantages of high cure rate and environmental protection.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms for preventing and controlling edible fungus Trichoderma contamination, and particularly relates to a biological composite Trichoderma inhibitor and a preparation method thereof. Background Art

[0002] Green mold disease caused by Trichoderma is the biggest threat in the production process of edible fungi. Chemical agents are usually used for prevention and control in production. Once improper pesticides are used for prevention and control during the fruiting period, pesticide residues will be caused. And the long-term use of a single chemical agent will enhance disease resistance, reduce the prevention and control effect, and the residue is large, posing a threat to human health and polluting the environment. In recent years, plant extracts such as wood vinegar, plant fumigants, and Jatropha curcas extracts that replace chemical pesticides have been developed at home and abroad to control Trichoderma hazards. Although the antibacterial effect is good, the above finished product manufacturing cost is high and the yield is low. Therefore, the use of microorganisms to prevent and control Trichoderma has a good application prospect. At present, there are microbial strains and antibiotics used to prevent and control Trichoderma, such as Bacillus subtilis, natamycin, etc. Although the microorganism has a good inhibitory effect on Trichoderma, it has not been reported that the microbial strain that can inhibit Trichoderma is compounded to achieve a better inhibitory effect. Summary of the invention

[0003] The purpose of the present invention is to solve the technical problems of low control effect, large residue and high manufacturing cost of existing agents for controlling Trichoderma, and to provide a biological composite Trichoderma inhibitor and a preparation method thereof.

[0004] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0005] A biological composite Trichoderma inhibitor is prepared by fermenting Bacillus subtilis ACCC11088 and Streptomyces jingyangensis ACCC40041 separately to obtain fermentation liquid, then evenly mixing the two fermentation liquids at a weight ratio of 1-5:5-1 to obtain the biological composite Trichoderma inhibitor; or evenly mixing the remaining filtrates after filtering the bacterial bodies of the two fermentation liquids at a weight ratio of 1-5:5-1 to obtain the biological composite Trichoderma inhibitor; wherein the bacterial count of Bacillus subtilis ACCC11088 in the biological composite Trichoderma inhibitor is 50 million / mL to 4 billion / mL, and the bacterial count of Streptomyces jingyangensis ACCC40041 is 50 million / mL to 2 billion / mL.

[0006] The preparation method of the biological composite Trichoderma inhibitor of the present invention comprises the following steps:

[0007] (1) Preparation of single strain fermentation broth:

[0008] Preparation of Bacillus subtilis ACCC11088 fermentation liquid: inoculating Bacillus subtilis ACCC11088 into a liquid culture medium under sterile conditions, fermenting and culturing at 30°C and 180r / min, and forming spores by microscopic examination of 80%, wherein the liquid culture medium comprises the following components in 1.0L of distilled water: 9-11g of peptone, 3-5g of beef extract, 4.5-5.5g of sodium chloride, and the pH value is adjusted to between 7.2 and 7.4;

[0009] Preparation of fermentation broth of Jingyang Streptomyces ACCC40041: Jingyang Streptomyces ACCC40041 is inoculated into Gao's liquid medium No. Ⅰ under sterile conditions, and fermented and cultured at 28° C. and 180 r / min for 5 to 7 days, wherein the Gao's liquid medium No. Ⅰ comprises the following components in 1.0 L of distilled water: soluble starch: 18 to 23 g, potassium nitrate 1 g, dipotassium hydrogen phosphate 0.4 to 0.6 g, magnesium sulfate heptahydrate 0.4 to 0.6 g, sodium chloride 0.4 to 0.6 g, ferrous sulfate heptahydrate 0.01 g, and the pH is adjusted to between 7.4 and 7.6;

[0010] (2) Preparation of composite Trichoderma inhibitor:

[0011] The fermentation liquid of Bacillus subtilis ACCC11088 and the fermentation liquid of Streptomyces jingyangensis ACCC40041 prepared in step (1) are mixed in a weight ratio of 1-5:5-1;

[0012] Alternatively, the fermentation liquid of Bacillus subtilis ACCC11088 and the fermentation liquid of Streptomyces jingyangensis ACCC40041 are filtered separately, and the filtrates thereof are mixed in a weight ratio of 1-5:5-1.

[0013] The composite Trichoderma inhibitor of the present invention is composed of two beneficial microorganisms, namely Bacillus subtilis ACCC11088 and Streptomyces jingyangensis ACCC40041 preserved by the Agricultural Microbiological Center of China Microbiological Culture Collection Committee. They can effectively inhibit the growth of edible fungi Trichoderma in the early stage of edible fungi hyphae growth, and will not inhibit the growth of edible fungi hyphae, and are non-toxic and harmless to humans and animals.

[0014] To further demonstrate the effect of the biological composite Trichoderma inhibitor of the present invention, the present invention conducted the following experiments:

[0015] 1. Isolate Trichoderma from the culture medium of diseased Pleurotus ostreatus, Lentinula edodes and Flammulina velutipes to provide pathogens for Trichoderma antagonism experiments. The specific steps are as follows:

[0016] 1. Pick the Trichoderma spores from the diseased oyster mushroom, shiitake mushroom and enoki mushroom culture materials or fruiting bodies on the PDA plate, place it at 26℃, observe the cultured plate, select the green colonies with a large number of growth for purification, pick the hyphae and streak them on the PDA plate, culture at 26℃, and after obtaining pure colonies, inoculate them into PDA slant test tubes and store them in a 4℃ refrigerator; culture the purified Trichoderma on the PDA medium at 26℃ in the dark, observe the morphological characteristics and color of the colonies every day, measure the colony diameter, pick the hyphae and spores for slide preparation, and observe the branching mode of conidiophores, the morphology of spore-producing cells and spores under a microscope;

[0017] 2. After separation and purification, a strain of Trichoderma was isolated from Lentinula edodes and numbered XG, two strains of Trichoderma were isolated from Pleurotus ostreatus and numbered M3 and M5, and a strain of Trichoderma was isolated from Flammulina velutipes and numbered JZG. The characteristics of each strain are as follows:

[0018] XG: On the PDA plate, the colony is initially white and velvety, and later a dense conidial area of ​​hyphae appears in a whorl shape, which is green to dark green in color. The conidiophores are arranged in a ring, and the secondary branches are single or in groups of 2-3, extending at a large angle. The branches are curved or wavy, and the base of the phialopsis is constricted, wider in the middle, and narrows from the middle to form a long neck. The conidia are spherical with thorns on the surface.

[0019] M3: The hyphae grow fast, the back is colorless to light yellow, the colony is white at first, then turns green, and there is a circle of obvious spore-producing area in the middle of the colony at a later stage. The conidiophores are multi-branched with large branching angles, the phialides are slender, opposite and whorled on the conidiophores, the terminal phialides are longer, and the conidia are elliptical or oval.

[0020] M5: The colony is white at first, then turns green. In the later stage, there is a circle of obvious conidia-producing area in the middle of the colony. The conidiophores are multi-branched with large branching angles. The phialides are slender, opposite and whorled on the conidiophores. The conidia are short columnar.

[0021] JZG: The hyphae grow very fast, and the back is colorless or dark yellow. The colony is white and velvety at first, and then turns green to dark green. In the later stage, a dense conidial area of ​​hyphae appears in a whorl shape. The conidiophores have few branches, and the secondary branches are single or in groups of 2-3, extending at a large angle, and the branches are slightly curved.

[0022] 2. Experiment on the treatment of edible fungi infected with Trichoderma

[0023] Experimental methods:

[0024] 1. Preparation of cultivation bags

[0025] The cottonseed husks and corn cobs used as raw materials for the cultivation of oyster mushrooms are subjected to a simple fermentation treatment (for about 5 days), bagged, and inoculated with oyster mushroom strains. When the oyster mushroom mycelium grows to about 3 to 5 cm, the oyster mushroom mycelium is ready for use.

[0026] 2. Preparation of various antagonistic strains

[0027] Prepare ordinary and Gao's liquid culture media respectively, culture in shake flasks at 28°C, and set aside after the bacterial liquid culture is completed.

[0028] 3. Re-inoculation of pathogens

[0029] After activating the M5, M3, XG and JZG pathogens, use a pipette to draw a quantitative amount of 0.1 ml and inject it into the bacteria bag. Culture it for 2 to 3 days and set it aside.

[0030] 4. Pathogen treatment

[0031] When the pathogen grows to 3-8 cm, use a syringe to absorb the bacterial solution of Bacillus subtilis ACCC11088 + Jingyang Streptomyces ACCC40041 stock solution, 10-fold dilution and filtrate and inject it into the pathogen site to ensure that the antagonistic bacterial solution contacts the pathogen, and measure the cure rate of each treatment.

[0032] Experimental results: The cure rates of the original solution treatment group against four Trichoderma pathogens reached 88%, 93%, 90%, and 91%; the cure rates of the ten-fold dilution treatment group against four Trichoderma pathogens reached 75%, 81%, 84%, and 84%; the cure rates of the bacterial filtrate treatment group against four Trichoderma pathogens reached 85%, 91%, 90%, and 91%; the cure rates of the blank control were 5%, 5%, 10%, and 9%. See Table 1 for specific data.

[0033] The above experiments show that the inhibition rate of the present invention on Trichoderma of Pleurotus ostreatus, Lentinula edodes and Flammulina velutipes reaches 85% to 91%.

[0034] Table 1 The therapeutic effect of the compound combination on XG, JZG, M3 and M5 pathogens

[0035] DETAILED DESCRIPTION

[0036] Example 1

[0037] A biological composite Trichoderma inhibitor is prepared by fermenting Bacillus subtilis ACCC11088 and Streptomyces jingyangensis ACCC40041 separately to prepare fermentation broth, and then evenly mixing the two fermentation broths at a weight ratio of 1:1 to prepare the biological composite Trichoderma inhibitor; wherein the bacterial count of Bacillus subtilis ACCC11088 in the biological composite Trichoderma inhibitor is 50 million / mL to 4 billion / mL, and the bacterial count of Streptomyces jingyangensis ACCC40041 is 50 million / mL to 2 billion / mL.

[0038] The specific preparation method comprises the following steps:

[0039] (1) Preparation of single strain fermentation broth:

[0040] Preparation of Bacillus subtilis ACCC11088 fermentation liquid: Bacillus subtilis ACCC11088 is inoculated into a common liquid culture medium under sterile conditions. The liquid culture medium comprises the following components in 1.0L of distilled water: 10g of peptone, 3g of beef extract, 5g of sodium chloride, the pH value is adjusted to between 7.2 and 7.4, and the fermentation is carried out at 30°C and 180r / min until 80% of spores are formed by microscopic examination.

[0041] Preparation of fermentation broth of Jingyang Streptomyces ACCC40041: Jingyang Streptomyces ACCC40041 was inoculated into Gao's liquid culture medium No. Ⅰ under sterile conditions, wherein the content of each component in 1.0L distilled water was: soluble starch: 20g, potassium nitrate 1g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate heptahydrate 0.5g, sodium chloride 0.5g, ferrous sulfate heptahydrate 0.01g, pH was adjusted to between 7.4 and 7.6, and fermentation was carried out at 28°C and 180r / min for 5 to 7 days.

[0042] (2) Preparation of composite Trichoderma inhibitor:

[0043] The fermentation liquid of Bacillus subtilis ACCC11088 and the fermentation liquid of Streptomyces jingyangensis ACCC40041 prepared in step (1) are mixed in a weight ratio of 1:1.

[0044] Example 2

[0045] A biological composite Trichoderma inhibitor is prepared by fermenting Bacillus subtilis ACCC11088 and Streptomyces jingyangensis ACCC40041 separately to prepare fermentation broth, and then evenly mixing the two fermentation broths at a weight ratio of 1:3 to prepare the biological composite Trichoderma inhibitor; wherein the bacterial count of Bacillus subtilis ACCC11088 in the biological composite Trichoderma inhibitor is 50 million / mL to 4 billion / mL, and the bacterial count of Streptomyces jingyangensis ACCC40041 is 50 million / mL to 2 billion / mL.

[0046] The specific preparation method comprises the following steps:

[0047] (1) Preparation of single strain fermentation broth:

[0048] The method of Example 1 was used to prepare a single-strain fermentation broth.

[0049] (2) Preparation of composite Trichoderma inhibitor:

[0050] The fermentation liquid of Bacillus subtilis ACCC11088 and the fermentation liquid of Streptomyces jingyangensis ACCC40041 prepared in step (1) are mixed in a weight ratio of 1:3.

[0051] Example 3

[0052] A biological composite Trichoderma inhibitor is prepared by fermenting Bacillus subtilis ACCC11088 and Streptomyces jingyangensis ACCC40041 to prepare fermentation broth, and then evenly mixing the two fermentation broths at a weight ratio of 1:5 to prepare the biological composite Trichoderma inhibitor; wherein the bacterial count of Bacillus subtilis ACCC11088 in the biological composite Trichoderma inhibitor is 50 million / mL to 4 billion / mL, and the bacterial count of Streptomyces jingyangensis ACCC40041 is 50 million / mL to 2 billion / mL.

[0053] The specific preparation method comprises the following steps:

[0054] (1) Preparation of single strain fermentation broth:

[0055] The method of Example 1 was used to prepare a single-strain fermentation broth.

[0056] (2) Preparation of composite Trichoderma inhibitor:

[0057] The fermentation liquid of Bacillus subtilis ACCC11088 and the fermentation liquid of Streptomyces jingyangensis ACCC40041 prepared in step (1) are mixed in a weight ratio of 1:5.

[0058] Example 4

[0059] A biological composite Trichoderma inhibitor is prepared by fermenting Bacillus subtilis ACCC11088 and Streptomyces jingyangensis ACCC40041 to prepare fermentation broth, and then evenly mixing the two fermentation broths at a weight ratio of 2:1 to prepare the biological composite Trichoderma inhibitor; wherein the bacterial count of Bacillus subtilis ACCC11088 in the biological composite Trichoderma inhibitor is 50 million / mL to 4 billion / mL, and the bacterial count of Streptomyces jingyangensis ACCC40041 is 50 million / mL to 2 billion / mL.

[0060] The specific preparation method comprises the following steps:

[0061] (1) Preparation of single strain fermentation broth:

[0062] The method of Example 1 was used to prepare a single-strain fermentation broth.

[0063] (2) Preparation of composite Trichoderma inhibitor:

[0064] The fermentation liquid of Bacillus subtilis ACCC11088 and the fermentation liquid of Streptomyces jingyangensis ACCC40041 prepared in step (1) are mixed in a weight ratio of 2:1.

[0065] Example 5

[0066] A biological composite Trichoderma inhibitor is prepared by fermenting Bacillus subtilis ACCC11088 and Streptomyces jingyangensis ACCC40041 separately to prepare fermentation broth, and then evenly mixing the two fermentation broths at a weight ratio of 5:1 to prepare the biological composite Trichoderma inhibitor; wherein the bacterial count of Bacillus subtilis ACCC11088 in the biological composite Trichoderma inhibitor is 50 million / mL to 4 billion / mL, and the bacterial count of Streptomyces jingyangensis ACCC40041 is 50 million / mL to 2 billion / mL.

[0067] The specific preparation method comprises the following steps:

[0068] (1) Preparation of single strain fermentation broth:

[0069] The method of Example 1 was used to prepare a single-strain fermentation broth.

[0070] (2) Preparation of composite Trichoderma inhibitor:

[0071] The fermentation liquid of Bacillus subtilis ACCC11088 and the fermentation liquid of Streptomyces jingyangensis ACCC40041 prepared in step (1) are mixed in a weight ratio of 5:1.

[0072] Example 6

[0073] A biological composite Trichoderma inhibitor is prepared by fermenting Bacillus subtilis ACCC11088 and Streptomyces jingyangensis ACCC40041 to prepare fermentation liquid, and then evenly mixing the remaining filtrates after filtering the bacterial bodies of the two fermentation liquids in a weight ratio of 1:1 to prepare the biological composite Trichoderma inhibitor; wherein the bacterial count of Bacillus subtilis ACCC11088 in the biological composite Trichoderma inhibitor is 50 million / mL to 4 billion / mL, and the bacterial count of Streptomyces jingyangensis ACCC40041 is 50 million / mL to 2 billion / mL.

[0074] The specific preparation method comprises the following steps:

[0075] (1) Preparation of single strain fermentation broth:

[0076] The method of Example 1 was used to prepare a single-strain fermentation broth.

[0077] (2) Preparation of composite Trichoderma inhibitor:

[0078] The fermentation broth of Bacillus subtilis ACCC11088 and the fermentation broth of Streptomyces jingyangensis ACCC40041 prepared in step (1) are filtered, and the filtrates are mixed in a weight ratio of 1:1 to prepare the method.

[0079] Example 7

[0080] A biological composite Trichoderma inhibitor is prepared by fermenting Bacillus subtilis ACCC11088 and Streptomyces jingyangensis ACCC40041 to prepare fermentation liquid, and then evenly mixing the remaining filtrates after filtering the bacterial bodies of the two fermentation liquids at a weight ratio of 1:2 to prepare the biological composite Trichoderma inhibitor; wherein the bacterial count of Bacillus subtilis ACCC11088 in the biological composite Trichoderma inhibitor is 50 million / mL to 4 billion / mL, and the bacterial count of Streptomyces jingyangensis ACCC40041 is 50 million / mL to 2 billion / mL.

[0081] The specific preparation method comprises the following steps:

[0082] (1) Preparation of single strain fermentation broth

[0083] The method of Example 1 was used to prepare a single-strain fermentation broth.

[0084] (2) Preparation of composite Trichoderma inhibitor:

[0085] The fermentation broth of Bacillus subtilis ACCC11088 and the fermentation broth of Streptomyces jingyangensis ACCC40041 prepared in step (1) are filtered, and the filtrates are mixed in a weight ratio of 1:2 to prepare the method.

[0086] Example 8

[0087] A biological composite Trichoderma inhibitor is prepared by fermenting Bacillus subtilis ACCC11088 and Streptomyces jingyangensis ACCC40041 to prepare fermentation liquid, and then evenly mixing the remaining filtrates after filtering the bacterial bodies of the two fermentation liquids at a weight ratio of 1:4 to prepare the biological composite Trichoderma inhibitor; wherein the bacterial count of Bacillus subtilis ACCC11088 in the biological composite Trichoderma inhibitor is 50 million / mL to 4 billion / mL, and the bacterial count of Streptomyces jingyangensis ACCC40041 is 50 million / mL to 2 billion / mL.

[0088] The specific preparation method comprises the following steps:

[0089] (1) Preparation of single strain fermentation broth:

[0090] The method of Example 1 was used to prepare a single-strain fermentation broth.

[0091] (2) Preparation of composite Trichoderma inhibitor:

[0092] The fermentation liquid of Bacillus subtilis ACCC11088 and the fermentation liquid of Streptomyces jingyangensis ACCC40041 prepared in step (1) are filtered, and the filtrates are mixed in a weight ratio of 1:4 to prepare the method.

[0093] Example 9

[0094] A biological composite Trichoderma inhibitor is prepared by fermenting Bacillus subtilis ACCC11088 and Streptomyces jingyangensis ACCC40041 to prepare fermentation liquid, and then evenly mixing the remaining filtrates after filtering the bacterial bodies of the two fermentation liquids at a weight ratio of 3:1 to prepare the biological composite Trichoderma inhibitor; wherein the bacterial count of Bacillus subtilis ACCC11088 in the biological composite Trichoderma inhibitor is 50 million / mL to 4 billion / mL, and the bacterial count of Streptomyces jingyangensis ACCC40041 is 50 million / mL to 2 billion / mL.

[0095] The specific preparation method comprises the following steps:

[0096] (1) Preparation of single strain fermentation broth:

[0097] The method of Example 1 was used to prepare a single-strain fermentation broth.

[0098] (2) Preparation of composite Trichoderma inhibitor:

[0099] The fermentation liquid of Bacillus subtilis ACCC11088 and the fermentation liquid of Streptomyces jingyangensis ACCC40041 prepared in step (1) are filtered, and the filtrates are mixed in a weight ratio of 3:1 to prepare the method.

[0100] Example 10

[0101] A biological composite Trichoderma inhibitor is prepared by fermenting Bacillus subtilis ACCC11088 and Streptomyces jingyangensis ACCC40041 to prepare fermentation liquid, and then evenly mixing the remaining filtrates after filtering the bacterial bodies of the two fermentation liquids at a weight ratio of 5:1 to prepare the biological composite Trichoderma inhibitor; wherein the bacterial count of Bacillus subtilis ACCC11088 in the biological composite Trichoderma inhibitor is 50 million / mL to 4 billion / mL, and the bacterial count of Streptomyces jingyangensis ACCC40041 is 50 million / mL to 2 billion / mL.

[0102] The specific preparation method comprises the following steps:

[0103] (1) Preparation of single strain fermentation broth:

[0104] The method of Example 1 was used to prepare a single-strain fermentation broth.

[0105] (2) Preparation of composite Trichoderma inhibitor:

[0106] The fermentation liquid of Bacillus subtilis ACCC11088 and the fermentation liquid of Streptomyces jingyangensis ACCC40041 prepared in step (1) are filtered, and the filtrates are mixed in a weight ratio of 5:1 to prepare the method.

Claims

1. An application of a biological composite Trichoderma inhibitor in preventing and controlling edible fungus Trichoderma contamination, characterized in that: The biocomposite Trichoderma inhibitor is prepared by fermenting Bacillus subtilis ACCC11088 and Streptomyces jingyangensis ACCC40041 separately to prepare fermentation liquid, and then the two fermentation liquids are evenly mixed in a weight ratio of 1-5:5-1 to prepare the biocomposite Trichoderma inhibitor; or the filtrates remaining after filtering the bacterial bodies of the two fermentation liquids are evenly mixed in a weight ratio of 1-5:5-1 to prepare the biocomposite Trichoderma inhibitor; wherein the bacterial count of Bacillus subtilis ACCC11088 in the biocomposite Trichoderma inhibitor is 50 million / mL to 4 billion / mL, and the bacterial count of Streptomyces jingyangensis ACCC40041 is 50 million / mL to 2 billion / mL.

2. The use of the biological composite Trichoderma inhibitor as claimed in claim 1 in preventing and controlling edible fungus Trichoderma contamination, characterized in that: The preparation method comprises the following steps: (1) Preparation of single strain fermentation broth: Preparation of Bacillus subtilis ACCC11088 fermentation broth: inoculating Bacillus subtilis ACCC11088 into a liquid culture medium under sterile conditions, fermenting and culturing at 30°C and 180r / min, and forming spores by microscopic examination of 80%, wherein the liquid culture medium comprises: 9-11g of peptone, 3-5g of beef extract, 4.5-5.5g of sodium chloride in 1.0L of distilled water, and the pH is adjusted to between 7.2 and 7.4; Preparation of fermentation broth of Jingyang Streptomyces ACCC40041: Jingyang Streptomyces ACCC40041 is inoculated into Gao's liquid medium No. Ⅰ under sterile conditions, and fermented and cultured at 28° C. and 180 r / min for 5 to 7 days, wherein the Gao's liquid medium No. Ⅰ comprises the following components in 1.0 L of distilled water: soluble starch: 18 to 23 g, potassium nitrate 1 g, dipotassium hydrogen phosphate 0.4 to 0.6 g, magnesium sulfate heptahydrate 0.4 to 0.6 g, sodium chloride 0.4 to 0.6 g, ferrous sulfate heptahydrate 0.01 g, and the pH is adjusted to between 7.4 and 7.6; (2) Preparation of composite Trichoderma inhibitor: The fermentation liquid of Bacillus subtilis ACCC11088 and the fermentation liquid of Streptomyces jingyangensis ACCC40041 prepared in step (1) are mixed in a weight ratio of 1-5:5-1; Alternatively, the fermentation liquid of Bacillus subtilis ACCC11088 and the fermentation liquid of Streptomyces jingyangensis ACCC40041 are filtered separately, and the filtrates thereof are mixed in a weight ratio of 1-5:5-1.

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