Use of alpha-glucosidases for enzymatic hydrolysis of disaccharides and oligosaccharides
By using α-glucosidase to hydrolyze disaccharides and oligosaccharides with α-1,5-glucosyl-fructose bonds, the problem of existing enzymes being unable to effectively handle this bond is solved, thus improving sugar decomposition efficiency and applicability to downstream processes.
CN106460023BActive Publication Date: 2026-02-24NUTRITION & BIOSCIENCES USA 4 INC
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Patent Information
- Application Number
- CN201580010439.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Priority Date
- 2015-02-25
- Filing Date
- 2015-02-26
- Publication Date
- 2026-02-24
- Estimated Expiration
- 2035-02-26
AI Technical Summary
Technical Problem
Existing α-glucosidases cannot effectively hydrolyze α-1,5-glucosyl-fructose bonds, which limits their application in disaccharides and oligosaccharides.
Method used
Alpha-glucosidases (such as transglucosidase and glucosylamylase) are used under specific conditions to hydrolyze disaccharides and oligosaccharides containing α-1,5-glucosyl-fructose bonds, thereby breaking these bonds through enzymatic reactions.
Benefits of technology
This method achieves efficient hydrolysis of α-1,5-glucosyl-fructose bonds, increases the yield of glucose and fructose, simplifies the downstream fermentation process, and reduces the content of byproducts.
✦ Generated by Eureka AI based on patent content.
Abstract
A method for hydrolyzing an alpha-1,5 glucosyl-fructose bond in a levansucrose, e.g., a sucrose, is disclosed. The method comprises contacting the sucrose with an alpha-glucosidase, e.g., a transglucosidase or a glucoamylase, under suitable conditions, during which the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose bond of the sucrose. The method is used, e.g., to reduce the amount of levansucrose in a filtrate isolated from a glucan synthesis reaction.
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