Detection kits for the detection and differentiation of a variety of common large and medium-sized tapeworms with dogs as their ultimate hosts

A detection kit and terminal host technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of pollution of the surrounding environment, cumbersome detection process, waste of materials, etc., and achieve sensitive, fast and accurate detection response. Etiological identification and high specificity of test results

CN106755458BActive Publication Date: 2020-04-03LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2020-04-03

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Abstract

The invention discloses a detection kit for detecting and distinguishing common large and medium-sized tapeworms taking a dog as a final host. The detection kit comprises four specific primer pairs, namely a taenia hydatigena specific primer pair, a taenia multiceps specific primer pair, a taenia pisiformis specific primer pair and a taenia elliptica specific primer pair. The kit has the benefits that the detection kit can determine if a detected sample is infected and determine a particular category of four kinds of parasites; the detection kit can be used for detecting an infection sample of an intermediate host and infection of the final host and can detect possible mixed infection of the final host. Comparison verification shows that the four primer pairs do not interfere with each other when the primer pairs are used in a PCR (polymerase chain reaction) system at the same time; therefore, the kit is high in efficiency, systematicness, economy and convenience, and applicable to quick and accurate etiological identification clinically and in laboratories.
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Description

technical field

[0001] The invention relates to the technical field of parasite differentiation and detection, in particular to a detection kit for detecting and distinguishing a variety of common large and medium-sized tapeworms with dogs as their final hosts. Background technique

[0002] There are many types and large numbers of tapeworms parasitic on livestock and poultry, belonging to the class Cestoidea of ​​the phylum Platyhelminthes, of which only tapeworms of the order Cyclophyllidea and Pseudophyllidea are infectious to livestock and poultry and humans . The distribution of tapeworms is extremely wide, and adults and metacestode larvae - tapeworm larvae can cause serious harm to humans and animals, and cause serious public health problems. Adults parasitize vertebrates, larvae mainly parasitize vertebrates, and can also parasitize invertebrates. For example, there are many kinds of cestodes in the round leaf order with dogs as their final hosts. Taenia multiceps ...

Examples

Embodiment 1

[0029] In this embodiment, dog feces were selected for detection of four kinds of tapeworm infection including Taenia alveosum. Take an appropriate amount of dog feces (2-5g), float the eggs and body fragments in the dog feces by saturated salt water flotation method, collect by centrifugation, and use the fecal DNA extraction kit to extract the whole genome DNA, and measure the concentration of the extracted DNA for later use. Prepare a compound PCR reaction solution in a PCR reaction tube, using a 50 μL reaction system, including 5 μL of 10×PCR Buffer, MgCl 2 (25mmol / μL) 5μL, upstream primer F1 for specific detection of Taenia vesicularis: 5'-AGT TCC ATA TTA TTT ACA GTT TTG TTA TTA C-3'(Th NO.1) 1μL (50μmol / mL) And downstream primer R1: 5'-TAA CAT AAT ACT TGA AGA CAC CCC CA-3'(ThNO.2) 1 μL (50 μmol / mL), upstream primer F2 for specific detection of Taenia polycephalum: 5'-GTT GTT GAT GTG GCT TAA GTTTTT GTG T-3'(Tm NO.1) 1μL (50μmol / mL) and downstream primer R2: 5'-TCT ATA AA...