A kind of preparation method of mushroom female seed culture medium and mushroom female seed culture medium

Abstract

The invention relates to a preparation method of a Lentinus edodes mother seed culture medium and a Lentinus edodes mother seed culture medium, and belongs to the technical field of culture medium preparation. On the basis of the conventional PDA medium, the Lentinus edodes mother seed culture medium prepared by the invention is added with the filtrate boiled with fine sawdust and bran, and the cellulose natural culture medium suitable for the growth of the Lentinus edodes mycelium is added, thereby effectively improving the Lentinus edodes. The ability of silk to decompose cellulose and lignin can effectively prevent the degeneration of the parent species of shiitake mushrooms, the mycelium has more branches at the tip, and has a certain rejuvenation effect on the parent species of shiitake mushrooms, which is conducive to maintaining the original characteristics of the parent species of shiitake mushrooms. When used to make parent seeds, the mycelium grows vigorously, is white and dense, and after inoculation, the mycelium grows faster, has a strong ability to decompose cellulose, and has strong disease resistance. The success rate of female seed inoculation is over 99%, which is suitable for promotion.

Description

technical field

[0001] The invention relates to a method for preparing a culture medium for female shiitake mushrooms and a culture medium for female mushrooms, belonging to the technical field of culture medium preparation. Background technique

[0002] The slope preservation method is a commonly used strain preservation method at present. This method is simple and easy, does not require special equipment, and can observe the mycelium growth of the preserved strain in real time. The mother species preserved by this method are generally preserved by conventional PDA medium.

[0003] However, the PDA medium belongs to the semi-synthetic agar medium. In this medium, although the mushroom hyphae can carry out normal vegetative growth, they cannot be used for fruiting. Over time, the inherent reproductive growth factors of shiitake mushrooms cannot be activated. At the same time, many extracellular enzymes in shiitake mushrooms, such as cellulolytic enzymes, hemicellulolytic en...

Examples

Embodiment 1

[0026] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:

[0027] 1) A, take 95g of broad-leaved forest fine sawdust and 5g of bran, wrap them with four layers of gauze respectively, place them in 500mL of water and boil for 30min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;

[0028] B. Take 160g of peeled potatoes, put them in 500mL of water and boil for 30 minutes until the potatoes are cooked but not rotten, and filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0029] 2) Mix potato filtered juice and cellulose filtered juice with 10 g of glucose and 16 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 35 minutes.

[0030] The medium of mushroom female species in this example was prepared by the above method.

Embodiment 2

[0032] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:

[0033] 1) A, take 90g of broad-leaved forest fine sawdust and 7g of bran, wrap them with four layers of gauze respectively, place them in 450mL water and boil for 20min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;

[0034] B. Take 150g of peeled potatoes, put them in 450mL of water and boil for 20 minutes until the potatoes are cooked but not rotten, and filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0035] 2) Mix potato filtered juice and cellulose filtered juice with 8 g of glucose and 15 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 40 minutes.

[0036] The medium of mushroom female species in this example was prepared by the above method.

Embodiment 3

[0038] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:

[0039] 1) A, take 100g of broad-leaved forest fine sawdust and 3g of bran, wrap them with four layers of gauze respectively, place them in 450mL of water and boil for 25min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;

[0040] B. Take 170g of peeled potatoes, put them in 450mL of water and boil for 25min until the potatoes are cooked but not rotten, filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0041] 2) Mix potato filtered juice and cellulose filtered juice with 12g of glucose and 17g of agar, add water to make up to 1000mL, and sterilize under high pressure steam at 121°C for 45min.

[0042] The medium of mushroom female species in this example was prepared by the above method.

technical field

[0001] The invention relates to a method for preparing a culture medium for female shiitake mushrooms and a culture medium for female mushrooms, belonging to the technical field of culture medium preparation. Background technique

[0002] The slope preservation method is a commonly used strain preservation method at present. This method is simple and easy, does not require special equipment, and can observe the mycelium growth of the preserved strain in real time. The mother species preserved by this method are generally preserved by conventional PDA medium.

[0003] However, the PDA medium belongs to the semi-synthetic agar medium. In this medium, although the mushroom hyphae can carry out normal vegetative growth, they cannot be used for fruiting. Over time, the inherent reproductive growth factors of shiitake mushrooms cannot be activated. At the same time, many extracellular enzymes in shiitake mushrooms, such as cellulolytic enzymes, hemicellulolytic en...

Examples

Embodiment 1

[0026] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:

[0027] 1) A, take 95g of broad-leaved forest fine sawdust and 5g of bran, wrap them with four layers of gauze respectively, place them in 500mL of water and boil for 30min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;

[0028] B. Take 160g of peeled potatoes, put them in 500mL of water and boil for 30 minutes until the potatoes are cooked but not rotten, and filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0029] 2) Mix potato filtered juice and cellulose filtered juice with 10 g of glucose and 16 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 35 minutes.

[0030] The medium of mushroom female species in this example was prepared by the above method.

Embodiment 2

[0032] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:

[0033] 1) A, take 90g of broad-leaved forest fine sawdust and 7g of bran, wrap them with four layers of gauze respectively, place them in 450mL water and boil for 20min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;

[0034] B. Take 150g of peeled potatoes, put them in 450mL of water and boil for 20 minutes until the potatoes are cooked but not rotten, and filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0035] 2) Mix potato filtered juice and cellulose filtered juice with 8 g of glucose and 15 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 40 minutes.

[0036] The medium of mushroom female species in this example was prepared by the above method.

Embodiment 3

[0038] The preparation method of the mother seed culture medium of shiitake mushroom in the present embodiment, comprises the steps:

[0039] 1) A, take 100g of broad-leaved forest fine sawdust and 3g of bran, wrap them with four layers of gauze respectively, place them in 450mL of water and boil for 25min, use two layers of gauze to filter to obtain the filtrate, which is the cellulose filtered juice;

[0040] B. Take 170g of peeled potatoes, put them in 450mL of water and boil for 25min until the potatoes are cooked but not rotten, filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0041] 2) Mix potato filtered juice and cellulose filtered juice with 12g of glucose and 17g of agar, add water to make up to 1000mL, and sterilize under high pressure steam at 121°C for 45min.

[0042] The medium of mushroom female species in this example was prepared by the above method.

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