One-step embryo culture medium and preparation method thereof

An embryo culture solution and acetylation technology, applied in the field of assisted reproduction, can solve problems such as death, nutritional insufficiency, excessive physical or chemical indicators, etc., and achieve the effect of complete morphology and high blastocyst formation rate

CN108251354BActive Publication Date: 2021-04-06成都艾伟孚生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2021-04-06

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Abstract

The invention belongs to the field of assisted reproduction, and in particular relates to a one-step embryo culture solution and a preparation method thereof. The composition used to prepare one-step embryo culture solution in the present invention, the composition is composed of (±)-α-lipoic acid, acetyl L-carnitine or acetyl L-carnitine hydrochloride, N-acetyl-L ‑cysteine ​​composition; the molar ratio of (±)‑α‑lipoic acid, acetyl L‑carnitine or acetyl L‑carnitine hydrochloride, N‑acetyl‑L‑cysteine ​​is 0.055‑ 0.098: 0.044‑0.078: 0.702‑0.858. The one-step embryo culture solution prepared by using the composition retains the active ingredients secreted by the embryo into the culture solution, can protect cells and inhibit the decomposition and utilization of harmful products, and avoid possible mechanical damage and culture environment during embryo transfer. The problem of low blastocyst formation rate caused by different stress responses.
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Description

technical field

[0001] The invention belongs to the field of assisted reproduction, and in particular relates to a one-step embryo culture solution and a preparation method thereof. Background technique

[0002] In Vitro Fertilization-Embryo Transfer In Vitro Fertilization-Embryo Transfer, referred to as IVF-ET, refers to the removal of eggs and sperm, in vitro fertilization, and then the embryo precursor - fertilized eggs are transplanted back into the mother's uterus to develop into fetuses. Test-tube babies are babies born by artificially fertilizing eggs and sperm outside the body and carrying out early embryonic development, and then transplanting them into the mother's womb.

[0003] Since the world's first "test-tube" baby was born in the UK on July 25, 1978, as of July 1, 2012, 5 million "test-tube" babies have been born. IVF has brought good news to more than 50 million infertile couples worldwide. However, more than three decades of clinical practice has not sign...

Examples

Embodiment 1

[0059] Embodiment 1 one-step embryo culture medium

[0060]

[0061]

Embodiment 2

[0062] Embodiment 2 one-step embryo culture medium

[0063]

Embodiment 3

[0064] Embodiment 3 one-step embryo culture medium

[0065]