Compositions and methods for vaccination against dengue virus in children and young adults

Abstract

Embodiments herein relate to compositions, methods and uses for inducing immune responses against all four dengue virus serotypes in children or young adults aged about 1 to about 20 years old. Some embodiments relate to compositions, including but not limited to dengue virus chimeras, which can be used alone or in combination with other constructs for vaccine compositions against all four dengue virus serotypes. In certain embodiments, the composition may include constructs of more than one dengue virus serotype at various concentrations or ratios, such as dengue ‑1 (DEN‑1) virus, dengue ‑2 (DEN‑2) virus, dengue ‑3 (DEN‑3) virus and / or dengue ‑4 (DEN‑4) virus to improve protection from infection in children and young adults. In certain embodiments, the virus of the formulation is limited to dengue virus serotypes. Other embodiments relate to methods for administering an immunogenic composition for dengue virus using a single, dual or other regimen, the immunogenic composition may include a chimeric dengue construct and a live attenuated dengue virus.

Description

[0001] Related applications

[0002] This PCT application claims the benefit under 35 USC §119(e) of U.S. Provisional Application Serial No. 62 / 322,167, filed April 13, 2016, which is incorporated herein by reference in its entirety for all purposes. Field of the Invention

[0003] Embodiments disclosed herein relate to compositions, methods and uses for inducing an immune response against all four dengue virus serotypes in children or young adults from about 1 year old to about 20 years old. Some embodiments relate to compositions that may include but are not limited to chimeric and non-chimeric flavivirus constructs, which may be used in vaccine compositions alone or in combination to induce an immune response against all four dengue virus serotypes. In certain embodiments, compositions may include constructs of more than one dengue virus serotype, such as dengue-1 (DEN-1) virus, dengue-2 (DEN-2) virus, dengue-3 (DEN-3) virus and / or dengue-4 (DEN-4) virus, to improve protection against dengue infection in children and young adults. Other embodiments relate to methods for administering a vaccine composition that may include a chimeric dengue construct and live attenuated dengue virus using a single, dual or other administration regimen. Background of the Invention

[0005] Dengue fever is a mosquito-borne disease caused by infection with the dengue virus. Dengue virus infection can cause debilitating and painful symptoms, including a sudden high fever, headache, joint and muscle pain, nausea, vomiting, and rash. To date, four dengue virus serotypes have been identified: dengue-1 (DEN-1), dengue-2 (DEN-2), or dengue-3 (DEN-3), and dengue-4 (DEN-4). Additional subtypes (e.g., DEN-5) may be discovered in the future. Dengue virus serotypes 1-4 can also cause dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). In the most severe cases, DHF and DSS can be life-threatening. Dengue virus causes 50 to 100 million cases of debilitating dengue fever, 500,000 cases of DHF / DSS, and more than 20,000 deaths each year, the majority of which are children. To date, there is no effective vaccine to prevent dengue fever and no drug treatment for the disease. Mosquito control efforts have been ineffective in preventing dengue outbreaks in endemic areas or in preventing further geographical spread of the disease. An estimated 3.5 billion people are at risk of dengue virus infection. In addition, dengue virus is a major cause of fever in travelers to endemic areas such as Asia, Central and South America, and the Caribbean. In addition, DHF / DSS is a major cause of serious illness and death in children in some Asian and Latin American countries.

[0006] All four dengue virus serotypes are endemic in tropical regions of the world and constitute the most important mosquito-borne viral threat to humans in tropical regions throughout the world. Dengue virus is primarily transmitted to humans by Aedes aegypti. Infection with one dengue virus serotype results in lifelong protection against reinfection of the serotype, but cannot prevent secondary infection with one of the other three dengue virus serotypes. In fact, the risk of severe disease (DHF / DSS) after previous infection with a dengue virus serotype results in a different serotype of secondary infection. Developing an effective vaccine (particularly in children and young people) is an important approach to preventing and controlling this global disease. SUMMARY OF THE INVENTION

[0008] Embodiments disclosed herein relate to compositions, methods, and uses of chimeric dengue virus constructs. In some embodiments, a composition may comprise a chimeric dengue virus construct alone or in combination with a live attenuated dengue virus having an attenuated dengue virus backbone having structural genes from at least one other dengue virus serotype. Other embodiments relate to a combination of at least one live attenuated virus and one or more chimeric dengue viruses containing structural elements for at least one other dengue virus serotype. Other embodiments may include a chimeric dengue composition having a modified DEN-2 backbone (e.g., PDK-53 as the starting backbone in P1 (passage-1) and using passage variability and selection of modified PDK-53, as noted for P2, P3...P8..P10, etc.) and one or more structural components of DEN-1, DEN-2, DEN-3, or DEN-4. In some embodiments, an immunogenic composition can be produced, wherein when introduced into a subject, the composition produces an immune response to one or more dengue viruses in the subject. Thus, the constructs encompassed herein can be produced and passaged, and each passage provides an attenuated dengue virus encompassed for use in a pharmaceutically acceptable vaccine composition.

[0009] Embodiments disclosed herein relate to compositions, methods and uses for inducing an immune response to all four dengue virus serotypes in children or young adults between the ages of about 1 and about 45 years. Current formulations are ineffective for young children, and there is an unmet need for dengue vaccines for use against dengue infection in all age groups. According to these embodiments, compositions and methods disclosed herein are used for children between the ages of about 1 and 20 years, about 1 and 15 years, about 1 and 11 years, or about 2 to about 9 years, or about 1 to about 5 years.

[0010] In certain embodiments, the chimeric dengue virus construct of the dengue virus serotype for vaccine can include passage 8 (P8) live, attenuated virus or chimeric virus, which has the nucleic acid sequence identified by SEQ ID NO:1,3,5 and 7 or the polypeptide sequence indicated by SEQ ID NO:2,4,6 and 8. It is encompassed herein that any passage of any live attenuated virus described herein can be used in immunogenic compositions to induce an immune response against the represented dengue virus serotype (e.g., serotype 1-4). According to these embodiments, the immunogenic composition comprising the live attenuated virus separated by P-8 can be administered to a subject to induce an immunogenic response against one or more dengue virus serotypes according to the construct selected. In addition, the live attenuated virus can be combined with one or more of these chimeric viruses. Consider this point for each live attenuated virus (e.g., African green monkey Vero cell production, hereinafter: Vero cell or other suitable cell line) separated / produced in each subsequent cell passage. Contemplated herein are any cell lines capable of producing dengue virus (eg, GMP approved) for use in propagating any viral constructs at manufacturing scale or, where appropriate, contemplated herein for subsequent use in vaccines or immunogenic compositions against dengue virus.

[0011] In other embodiments, the compositions encompassed herein can be combined with other immunogenic compositions directed against other flaviviruses, such as Zika virus, West Nile virus, Japanese encephalitis, St. Louis encephalitis virus, yellow fever virus, or any other flavivirus chimeric construct and / or live attenuated virus. In certain embodiments, a single composition can be used against multiple flaviviruses. In some embodiments, even if the vaccine composition is limited to dengue virus constructs and live attenuated dengue virus, the compositions disclosed herein are capable of inducing an immune response against Zika virus.

[0012] In certain embodiments, the immunogenic compositions of the invention may include chimeric dengue viruses directed against one or more of DEN-1, DEN-2, DEN-3, and / or DEN-4, alone or in combination with a live, attenuated dengue virus composition.

[0013] In other embodiments, the construct may include a construct with an adaptive mutation in the structural or non-structural region of the virus, the adaptive mutation increasing growth or production when introduced into a subject without affecting the attenuation or safety of the virus. In certain embodiments, any encompassed chimeric dengue virus construct may include a live attenuated DEN-2 virus with a specific mutation used as a backbone, wherein the live attenuated DEN-2 PDK virus may further include one or more of the prM (premembrane) and E (envelope) structural proteins of other dengue virus serotypes. In addition, the DEN-2 backbone may include additional mutations or the reversion of mutations to enhance the immune response to a predetermined composition in the subject when administered (e.g., chimeric dengue virus 2 / 1, 2 / 3, or 2 / 4).

[0014] In some embodiments, the structural protein genes may include the prM and E genes of DEN-1, DEN-2, DEN-3, or DEN-4 with one or two reversions on the DEN-2 backbone to improve immunogenicity. For example, in certain embodiments, the dengue constructs may include those constructs designated TDV-1-A, TDV-2-F, TDV-3-F, and TDV-4-F (see Examples), wherein the DEN-2 backbone has one or more reversions to wild-type DEN-2 (e.g., in non-coding regions (NCRs) or non-structural regions (NS1, etc.) or other mutations not found in P1 or other previously passaged viruses) from live, attenuated DEN-2 viruses previously demonstrated to be safe and effective for inducing an immune response. The live, attenuated DEN-2 viruses of the present application are improved versions of the live, attenuated DEN-2 viruses initially used. The chimeric constructs of some embodiments disclosed herein may include a modified attenuated DEN-2PDK-53 backbone having one or more structural proteins of a second dengue virus serotype, wherein the structural proteins may contain additional mutations to increase the immunogenic response to the chimeric construct. In some embodiments, certain mutations obtained by attenuating DEN-2PDK-53 may be reverted to a control or another amino acid to generate a chimeric construct that differs from the P1 construct, which may result in increased immunogenicity, increased growth, increased plaque size without affecting vaccine safety and attenuation and may affect the growth and / or replication of the live attenuated virus.

[0015] In other embodiments, the live attenuated DEN-2 genome can be used to generate chimeric constructs of dengue virus serotype 1 (DEN-1), dengue virus serotype 3 (DEN-3), and dengue virus serotype 4 (DEN-4), wherein one or more structural protein genes of the DEN-2 viral genome can be replaced with one or more structural protein genes of DEN-1, DEN-3, or DEN-4, respectively. In some embodiments, the structural proteins can comprise the C, prM, or E proteins of the third dengue virus. In certain embodiments, the structural protein genes can comprise the prM and E genes of DEN-1, DEN-3, or DEN-4. According to these embodiments, these chimeric viruses can express the surface antigens of DEN-1, DEN-3, or DEN-4 while retaining the attenuated phenotype of the parental attenuated DEN-2. Furthermore, the chimeric constructs disclosed herein for use in childhood and young adult vaccines may include chimeric constructs of DEN-4, DEN-2, DEN-1, and DEN-3 using an attenuated DEN-2 virus as a backbone to express DEN-1, DEN-3, and DEN-4 surface antigens.

[0016] In certain embodiments, the composition of the present invention may include a composition that may include a single chimeric dengue virus construct disclosed herein and a pharmaceutically acceptable carrier or excipient. In other embodiments, the composition disclosed herein may include two or more, or three or more chimeric dengue virus constructs and a pharmaceutically acceptable carrier or excipient. According to these embodiments, one or more dengue virus chimeric constructs encompassed herein may be combined with one or more live attenuated dengue viruses. In certain embodiments, the live attenuated virus may be a live attenuated DEN-2 virus, wherein the reply to wild-type amino acids in NCR, NS1 region or other regions increases immune response for the improved live attenuated dengue virus construct used in the preparaton disclosed herein, increases viral growth or other improvements.

[0017] In certain embodiments, live attenuated dengue virus can include mutations or substitutions of nucleotides 5'NCR-57-T, NS1-53-Asp, and NS3-250-Val of DENV-2, which can be shared by the common PDK-53 virus-specific genetic background of four TDV construct viruses. The genetic sequences of the three attenuated loci and the in vitro and in vivo attenuated phenotypes of these vaccine candidates previously established have been carefully monitored for the TDV seeds manufactured by cGMP. Disclosed herein are strategies for producing master virus seeds (MVS) for the preparation of dengue virus vaccine compositions and other related passages of dengue virus. These MVS can be used to prepare clinical materials and ultimately to prepare commercial vaccine supplies.

[0018] In certain embodiments, the immunogenic composition may comprise a trivalent or quadrivalent formulation capable of inducing an immune response to at least three or all four dengue virus serotypes in children and young adults. For example, the immunogenic composition may comprise a pre-master virus seed (Pre-Master Virus Seed), a master virus seed (MVS), a working virus seed (WVS), and a bulk virus seed (BVS) construct for each of the four dengue virus serotypes. In other embodiments, the immunogenic composition may comprise one or more polynucleotides having a nucleic acid sequence encoding a modified live attenuated dengue-2 virus serotype represented by SEQ ID NO: 9 (pre-major), 11 (MVS), 13 (WVS) or 15 (BVS); a dengue-1 / dengue-2 chimeric polynucleotide having a nucleic acid sequence encoding a nonstructural protein from a modified live attenuated dengue-2 virus serotype and a structural protein from a dengue-1 virus serotype represented by SEQ ID NO: 1 (pre-major), 3 (MVS), 5 (WVS) or 7 (BVS); a dengue-3 / dengue-2 chimeric polynucleotide having a nucleic acid sequence encoding a nonstructural protein from a modified live attenuated dengue-2 virus serotype and a structural protein from a dengue-3 virus serotype represented by SEQ ID NO: NO: 17 (pre-major), 19 (MVS), 21 (WVS) or 23 (BVS); dengue-4 / dengue-2 chimeric polynucleotide having a nucleic acid sequence encoding a non-structural protein from a modified live attenuated dengue-2 virus serotype and a structural protein from a dengue-4 virus serotype, the nucleic acid sequence being represented by SEQ ID NO: 25 (pre-major), 27 (MVS), 29 (WVS) or 31 (BVS). Some embodiments disclose immunogenic compositions capable of eliciting an immune response against one, two, three or all four dengue virus serotypes in children or young adults.

[0019] In certain embodiments, the immunogenic composition may include a formulation wherein the concentration ratio of the polynucleotide encoding the modified live attenuated dengue-2 virus serotype in the composition is at least 0.5-1 log PFU lower than the log (log) phage forming units (PFU) of one or more dengue virus polynucleotides. In other embodiments, the immunogenic composition may include a formulation wherein the concentration ratio of the polynucleotide encoding the modified live attenuated dengue-2 virus serotype in the composition is at least 1.5 or greater than 2 or 3 log PFU lower than the log phage forming units (PFU) of one or more polynucleotides.

[0020] In certain embodiments, the immunogenic composition for dengue, alone or in combination with other viral vaccines, can be a mixture of polypeptides and polynucleotides. According to these embodiments, the immunogenic composition can comprise the polynucleotides described herein or other flavivirus polynucleotides with or without the various polypeptides encoded by the polynucleotides. For example, the polypeptides may include polypeptides corresponding to modified live attenuated dengue-2 virus serotypes represented by SEQ ID NO: 10 (pre-major), 12 (MVS), 14 (WVS) or 16 (BVS); polypeptides corresponding to dengue-1 / dengue-2 chimeric polynucleotides represented by SEQ ID NO: 2 (pre-major), 4 (MVS), 6 (WVS) or 8 (BVS); polypeptides corresponding to dengue-3 / dengue-2 chimeric polynucleotides represented by SEQ ID NO: 18 (pre-major), 20 (MVS), 22 (WVS) or 24 (BVS); and polypeptides corresponding to dengue-4 / dengue-2 chimeric polynucleotides represented by SEQ ID NO: 26 (pre-major), 28 (MVS), 30 (WVS) or 32 (BVS).

[0021] In certain embodiments, the immunogenic compositions disclosed herein may include polypeptides and / or polynucleotides at various concentration ratios, including a ratio of 4:4:5:5 plaque forming units (PFU) of dengue-1 / dengue-2 chimera to a modified live attenuated dengue-2 virus serotype to dengue-3 / dengue-2 chimera to dengue-4 / dengue-2 chimera. In other embodiments, the ratio of dengue-1 / dengue-2 chimera to a modified live attenuated dengue-2 virus serotype to the dengue-3 / dengue-2 chimera to the dengue-4 / dengue-2 chimera may be about 5:4:5:5 log PFU. Other ratios contemplated herein may include compositions in which live attenuated dengue-2 and / or dengue-1 chimeric viruses are reduced compared to at least one of dengue-3 or dengue-4 chimera. In other embodiments, the tetravalent formulation ratio can be about 5:3:5:5, 3:3:5:5, or any ratio that, in certain instances, has lower live, attenuated Dengue-2 or reduced presentation or both live, attenuated Dengue-2 and Dengue-1 / Dengue-2 chimera in the formulation.

[0022] In other embodiments, the compositions disclosed herein may be pharmaceutical compositions comprising one or more immunogenic compositions disclosed herein and a pharmaceutically acceptable excipient. In certain embodiments, the pharmaceutical compositions may comprise immunogenic compositions comprising live attenuated dengue virus and / or dengue chimeras having polynucleotides and polypeptides against one or more dengue serotypes.

[0023] Other embodiments may include kits having at least one immunogenic composition of the present disclosure and at least one container for use against dengue virus infection in children and young adults.

[0024] Certain embodiments include methods for immunizing children and young adults against at least one to all four dengue virus serotypes. The methods may comprise administering to a subject one or more doses of a pharmaceutical composition disclosed herein. According to these embodiments, the ratio having these characteristics may comprise a live attenuated virus and / or chimera that induces an immune response against all four dengue virus serotypes in the subject.

[0025] In certain embodiments, the method may comprise administering to a child or young adult an immunogenic composition comprising, in addition to the polynucleotide, one or more polypeptides encoded by the polynucleotides disclosed herein. The method may comprise administering to a child or young adult an immunogenic composition comprising, in addition to the polynucleotide, one or more polypeptides encoded by the polynucleotides disclosed herein. The method may comprise administering to a subject an immunogenic composition comprising one or more of the following: a polypeptide represented by SEQ ID NO: 10 (pre-major), 12 (MVS), 14 (WVS), or 16 (BVS); a polypeptide represented by SEQ ID NO: 2 (pre-major), 4 (MVS), 6 (WVS), or 8 (BVS); a polypeptide represented by SEQ ID NO: 18 (pre-major), 20 (MVS), 22 (WVS), or 24 (BVS); and a polypeptide represented by SEQ ID NO: 26 (pre-major), 28 (MVS), 30 (WVS), or 32 (BVS). In certain embodiments, the method may comprise administering to a subject aged between about 1 and about 20 years an immunogenic composition comprising all four dengue virus serotypes represented in the immunogenic composition disclosed herein and eliciting an immune response to all four dengue virus serotypes in the subject (a tetravalent formulation). In other embodiments, the methods can include administering an immunogenic composition disclosed herein to a subject ranging in age from about 1 year to about 45 years.

[0026] In certain embodiments, the method may include administering one or more tetravalent formulations of the present disclosure to a subject by any acceptable means, including, for example, subcutaneous, intravenous, intradermal, transdermal, oral, by inhalation, vaginally, topically, intranasally, or rectally. According to these embodiments, the formulation disclosed herein may be administered to a subject in a single dose or in two or more doses. In some embodiments, the immunogenic formulation disclosed herein may be administered within about 90 days of each other, within about 60 days of each other, within about 30 days of each other, and within less than about 30 days of each other. In some embodiments, the immunogenic formulation disclosed herein may be administered to a child or young adult on day 0 and administered again about 3 months after the first administration.

[0027] In certain embodiments, the tetravalent formulations disclosed herein can elicit an immune response against all four dengue virus serotypes in at least 60% of children and young adults who have received one or more doses of the tetravalent composition. Sequence Listing <110> Takeda Vaccine Co., Ltd. Wallace, Derek <120> Compositions and methods for vaccinating children and young adults against dengue virus <130> 478484.265 <150> XXXXXXXXX <151> 2017-04-13 <160> 34 <170> PatentIn version 3.5 <210> 1 <211> 10723 <212> DNA <213> Artificial sequence <220> <223> Dengue virus serotype 1, predominant <400> 1 agttgttagt ctacgtggac cgacaaagac agattctttg agggagctaa gctcaatgta 60 gttctaacag ttttttaatt agagagcaga tctctgatga ataaccaacg gaaaaaggcg 120 aaaaacacgc ctttcaatat gctgaaacgc gagagaaacc gcgtgtcgac tgtgcaacag 180 ctgacaaaga gattctcact tggaatgctg cagggacgag gaccattaaa actgttcatg 240 gccctggtgg cgttccttcg tttcctaaca atcccaccaa cagcagggat attgaagaga 300 tggggaacaa ttaaaaaatc aaaagctatt aatgttttga gagggttcag gaaagagatt 360 ggaaggatgc tgaacatctt gaataggaga cgcagatctg caggcatgat cattatgctg 420 attccaacag tgatggcgtt ccatttaacc acgcgtgggg gagagccgca tatgatagtt 480 agcaagcagg aaagaggaaa gtcacttttg ttcaagacct ctgcaggtgt caacatgtgc 540 accctcattg cgatggattt gggagagttg tgtgaggaca cgatgaccta 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tgctgacatg gctaggatta 2340 aattcaagga acacgtccct ttcgatgatg tgcatcgcag ccggcattgt gacactgtat ttgggagtca tggtgcaggc cgatagtggt tgcgttgtga gctggaaaaa caaagaactg aaatgtggca gtgggatttt catcacagac aacgtgcaca catggacaga acaatacaag ttccaaccag aatccccttc aaaactagct tcagctatcc agaagccca tgaagaggac 2580 atttgtggaa tccgctcagt aacagactg gagaatctga tgtggaaca aaacacca 2640 gattgaatc acattctatc agaaaatgag gtgaagttaa ctattatgac aggagacatc 2700 aaaggaatca tgcaggcagg aaacgatct ctgcggccctc agcccactga gctgaagtat 2760 tcatggaaaa catggggcaa agcaaaatg ctctctacag agtctcataa ccagaccttt 2820 ctcattgatg gccccgaac agcagaatgc cccacaca atagagcttg gattcgttg 2880 gaagttgaag actatggctt tggagtattc accaccaata tattggctaaa attgaaagaa 2940 aaaacaggatg tattctgcga ctcaaactc atgtcagcgg ccaaaaga caacagagcc 3000 gtccatgccg attgggtta ttggatagaa agtgcactca atgacacatg gaagatagag 3060 aaagcctctt tcattgaagt taaaaactgc cactggccaa atcacacac cctctggagc 3120 aatggagtgc tagaagtga gatgatatt ccaagaatc tcgctggacc agtgtctca 3180 cacaactata 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attackcattag cattgacgat caaggctc 4080 aatccaacag ctatttttct aaaaccctc tcaagaacca gcaagaaag gagctggcca 4140 ttaaatgagg ctatcatggc agtcgggatg gtgagcatttt tagccagttc tctcctaaaa 4200 aatgatattc ccatgacagg accattagtg gctggaggc tctcactgt gtgctacgtg 4260 ctcactggac gatcggccga ttggactg gagagagcag ccgatgtca atgggagac 4320 caggcagaga tatcaggaag cagtccaatc ctgtcaataa caatcaga agatggtagc 4380 atgtcgataa aaatgaaga ggagatcaa acactgacca tactcattag aacaggattg 4440 ctggtgatct caggacttt tctgtatca ataccaatca cggcagcagc atggtacctg 4500 tgggaagtga agaaacaacg ggccggagta tgtgggatg ttccttcacc cccacccatg 4560 ggaaaggctg aactggaga tggactt agattaagc aaaagggat tcttggatat 4620 tcccagatcg gagccggagt ttacaagaa ggaacattcc atacaatgtg gcatgtcaca 4680 cgtggcgctg ttctaatgca taaggaaag aggattgaac catcatgggc ggacgtcaag 4740 aagacctaa tatcatatgg aggaggctgg aagttagaag gagaatgaa ggaggagaa 4800 gaagtccagg tattggcact ggagcctgga aaaaatccaa gagccgtcca aacgaaacct 4860 ggtctttca aaaccaacgc cggaacaata ggtgctgtat ctctggactt ttctcctgga 4920 acgtcaggat ctccaattat cgacaaaaa ggaaagttg tggtcttta tggtaatggt 4980 gttgttacaa ggagtggagc atatgtgagt gctatagccc agactgaaa aagcattgaa 5040 gaacccag agatcgaga tgacattttc cgaaagagaa gactgaccat catggaccctc 5100 cacccaggag cgggaagac gagagatac cttccggcca tagtcagaga agcttaaaa 5160 cggggtttga gacattaat cttggccccc actagagttg tggcagctga aatggaggaa 5220 gcccttagag gacttccaat aagataccag accccagcca tcagagctgt gcacaccggg 5280 cgggagatg tggacctaat gtgtcatgcc accattacca tgaggctgct atcaccagtt 5340 agagtgccaa actacaacct gattatcatg gacgaagccc atttcacaga cccagcaagt 5400 atagcagcta gaggatacat ctcaactcga gtggagatgg gtgaggcagc tgggatttt atgacagcca ctccccggg aagcagagac ccattttcctc aagcaatgc accaatcata gatgaagaaa gagaaatccc tgaacgctcg tggaattccg gacatgaatg ggtcacggat tttaaaggga agactgtttg gttcgttcca agtataaaag caggaaatga tatagcagct tgcctgagga aaaatggaaa gaaagtgata caactcagta ggaagacctt tgattctgag tatgtcaaga ctagaaccaa tgattgggac ttcgtggtta caactgacat ttcagaaatg ggtgccaatt tcaaggctga gagggttata gaccccagac gctgcatgaa accagtcata ctaacagatg gtgaagagcg ggtgattctg gcaggaccta tgccagtgac ccactctagt gcagcacaaa gaagagggag aataggaaga aatccaaaaa atgagaatga ccagtacata tacatggggg aacctctgga aaatgatgaa gactgtgcac actggaaaga agctaaaatg ctcctagata acatcaacac gccagaagga atcattccta gcatgttcga accagagcgt gaaaggtgg atgccattga tggcgaatac cgcttgagag gagaagcaag gaaaaaccttt gtagacttaa tgagaagagg agacctacca gtctggttgg cctacagagt ggcagctgaa 6180 ggcatcaact acgcagacag aaggtggtgt tttgatggag tcaagaacaa ccaaatccta 6240 gaaaaacg tggaagttga aatctggaca aaagaagggg aaaggaagaa attgaaaccc 6300 agatggttgg atgctaggat ctattctgac ccactggcgc taaaagaatt taggaattt 6360 gcagccggaa gaaagtctct gaccctgaac ctaatcacag aaatgggtag gctcccaacc 6420 ttcatgactc agaagcaag agacgcactg gacaacttag cagtgctgca cacggctgag 6480 gcaggtggaa gggcgtacaa ccatgctctc agtgaactgc cggagaccct ggagacattg 6540 cttttactga cacttctggc tacagtcacg ggagggatct ttttattctt gatgagcgca 6600 aggggcatag ggaagatgac cctgggaatg tgctgcataa tcacggctag catcctccta 6660 tggtacgcac aaatacagcc acactggata gcagcttcaa taatactgga gttttttctc 6720 atagttttgc ttattccaga acctgaaaaa cagagaacac cccaagacaa ccaactgacc 6780 tacgttgtca tagccatcct cacagtggtg gccgcaacca tggcaaacga gatgggtttc 6840 ctagaaaaaa cgaagaaga tctcggattg ggaagcattg caacccagca acccgagagc 6900 aacatcctgg acatagatct acgtcctgca tcagcatgga cgctgtatgc cgtggccaca 6960 acatttgtta caccaatgtt gagacatagc attgaaaatt cctcagtgaa tgtgtcccta 7020 acagctatag ccaaccaagc cacagtgtta atgggtctcg ggaaaggatg gccattgtca 7080 aagatggaca tcggagttcc ccttctcgcc attggatgct actcacaagt caacccata 7140 actctcacag cagctctttt cttattgta gcacattatg ccatcatagg gccaggactc 7200 caagcaaag caccagaa agctcagaaa agagcagcgg cgggcatcat gaaaaaccca 7260 actgtcgatg gaataacagt gattgaccta gatccaatac cttatgatcc gaagtttgaa 7320 aagcagttgg gatagtaat gctcctagtc ctctgcgtga ctcaagtatt gatgatgagg 7380 actacatggg ctctgtgtga ggctttaacc ttagctaccg ggcccactc cacattgtgg 7440 gaaggaaatc cagggaggtt ttggacact accattgcgg tgtcaatggc tacattttt 7500 agagggagtt acttggccgg agctggactt ctctttcta ttatgagaa cacaccac 7560 acaagaaggg gaactggcaa cataggagag acgcttggag agaatggaa aagccgattg 7620 aacgcattgg gaaaaagtga attccagatc tacaagaaa gtggaatcca ggaagtggat 7680 agaaccttag caaagaagg cattaaaaga ggagaaacgg accacacgc tgtgtcgcga 7740 ggctcagcaa aactgagatg gttcgttgag agaaacatgg tcacaccaga agggaagta 7800 gtggacctcg gttgtggcag aggaggctgg tcatactatt gtggaggact aagaatgta 7860 agagaagtca aaggcctaac aaaaggagga ccaggacacg aagaacccat ccccatgtca 7920 acatagggt ggaatctagt gcgtcttcaa agtggagttg acgttttctt catcccgcca 7980 gaaaagtgtg acacattatt gtgtgacata ggggagtcat caccaatcc cacagtggaa 8040 gcaggacgaa cactcagagt ccttaactta gtagaaaatt ggttgacaa cacactca 8100 tttgcataa aggttctcaa cccatatatg ccctcagtca tagaaaaat ggaagcacta 8160 haaggaaat atggagc cttagtgagg aatccactct cacgaactc cacacatgag 8220 atgtactggg tatccaatgc ttccgggaac atagtgtcat cagtgaacat gatttcagg 8280 atgttgatca acagatttac aatgagatac aagaaagcca cttacgagcc ggatgttgac 8340 ctcggaagcg gaacccgtaa catcgggatt gaaagtgaga taccaaacct agatataatt 8400 gggaaaagaa tagaaaaaat aaagcaagag catgaaacat catggcacta tgaccaagac 8460 cacccataca aaacgtgggc ataccatggt agctatgaaa caaaacagac tggatcagca 8520 tcatccatgg tcaacggagt ggtcaggctg ctgacaaaac cttgggacgt cgtccccatg 8580 gtgacacaga tggcaatgac agacacgact ccatttggac aacagcgcgt ttttaaagag 8640 aaagtggaca cgagaaccca agaaccgaaa gaaggcacga agaaactaat gaaaataaca 8700 gcagagtggc tttggaaaga attagggaag aaaaagacac ccaggatgtg caccagagaa 8760 gaattcacaa gaaaggtgag aagcaatgca gccttggggg ccatattcac tgatgagaac 8820 aagtggaagt cggcacgtga ggctgttgaa gatagtaggt tttgggagct ggttgacaag 8880 gaaaggaatc tccatcttga aggaaagtgt gaaacatgtg tgtacaacat gatgggaaaa 8940 agagagaaga agctagggga attcggcaag gcaaaaggca gcagagccat atggtacatg 9000 tggcttggag cacgcttctt agagtttga gccctaggat tctttaatga agatcactgg 9060 ttctccagag agaactccct gagtggagtg gaaggagaag ggctgcacaa gctaggttac 9120 attctaagag acgtgagcaa gaagaggga ggagcaatgt atgccgatga caccgcagga 9180 tgggatacaa gatcacacact agaagaccta aaaaatgaag aaatggtaac aaaccacatg 9240 gaaggagaac acaagaact agccgaggcc atttcaac taacgtacca aaacaggtg 9300 gtgcgtgtgc aaagaccaac accaagaggc acagtaatgg acatcatatc gagagagac 9360 haaagaggta gtggacaagt tggcacctat ggactcaata ctttcaccaa tatggaagcc 9420 caactaatca gagatgga gggagaagga gtctttaaaa gcattcagca cctaacaatc 9480 acagaagaaa tcgctgtgca aactggtta gcaagagtgg ggcgcgaaag gttatcaaga 9540 atggccatca gtggagatga ttgtgttgtg aaacctttag atgacaggtt cgcaagcgct 9600 ttaacagctc taaatgacat gggaagatt aggaagaca tacacaatg ggaaccttca 9660 agaggatgga atgattggac acaagtgccc ttctgttcac accattcca tgagttaatc 9720 atgaaagacg gtcgcgtact cgttgttcca tgtagaaacc aagatgaact gattggcaga 9780 gcccgaatct cccaaggagc agggtggtct ttgcgggaga cggcctgttt ggggaagtct 9840 tacgcccaaa tgtggagctt gatgtacttc cacagacgcg acctcaggct ggcggcaaat 9900 gctatttgct cggcagtacc atcacattgg gttccaacaa gtcgaacaac ctggtccata 9960 catgctaaac atgaatggat gacaacggaa gacatgctga cagtctggaa cagggtgtgg 10020 attcaagaaa acccatggat ggaagacaaa actccagtgg aatcatggga ggaaatccca 10080 tacttgggga aaagagaaga ccaatggtgc ggctcattga ttgggttaac aagcagggcc 10140 acctgggcaa agaacatcca agcagcaata aatcaagtta gatcccttat aggcaatgaa 10200 gaatacacag attacatgcc atccatgaaa agattcagaa gagaagagga agaagcagga 10260 gttctgtggt agaaagcaaa actaacatga aacaaggcta gaagtcaggt cggattaagc 10320 catagtacgg aaaaaactat gctacctgtg agccccgtcc aaggacgtta aaagaagtca 10380 ggccatcata aatgccatag cttgagtaaa ctatgcagcc tgtagctcca cctgagaagg 10440 tgtaaaaaat ccgggaggcc acaaaccatg gaagctgtac gcatggcgta gtggactagc 10500 ggttagagga gacccctccc ttacaaatcg cagcaacaat ggggccccaa ggcgagatga 10560 agctgtagtc tcgctgggaag gactagaggt tagaggac ccccccgaaa caaaaaacag 10620 catattgacg ctgggaaaga ccagagatcc tgctgtctcc tcagcatcat tccaggcaca 10680 gaacgccaga aaatggaatg gtgctgttga atcaacaggt tct 10723 <210> 2 <211> 3391 <212> PRT <213> artificial sequence <220> <223> Dengue fever virus serotype 1, the main one <400> 2 Met Asn Asn Gln Arg Lys Lys Ala Lys Asn Thr Pro Phe Asn Met Leu 1 5 10 15 Lys Arg Glu Arg Asn Arg Val Ser Thr Val Gln Gln Leu Thr Lys Arg 20 25 30 Phe Ser Leu Gly Met Leu Gln Gly Arg Gly Pro Leu Lys Leu Phe Met 35 40 45 Ala Leu Val Ala Phe Leu Arg Phe Leu Thr Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Lys Arg Trp Gly Thr Ile Lys Lys Ser Lys Ala Ile Asn Val 65 70 75 80 Leu Arg Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn 85 90 95 Arg Arg Arg Arg Ser Ala Gly Met Ile Ile Met Leu Ile Pro Thr Val 100 105 110 Met Ala Phe His Leu Thr Thr Arg Gly Gly Glu Pro His Met Ile Val 115 120 125 Ser Lys Gln Glu Arg Gly Lys Ser Leu Leu Phe Lys Thr Ser Ala Gly 130 135 140 Val Asn Met Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Met Thr Tyr Lys Cys Pro Arg Ile Thr Glu Ala Glu Pro Asp 165 170 175 Asp Val Asp Cys Trp Cys Asn Ala Thr Asp Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Ser Gln Thr Gly Glu His Arg Arg Asp Lys Arg Ser Val Ala 195 200 205 Leu Ala Pro His Val Gly Leu Gly Leu Glu Thr Arg Ala Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys Gln Ile Gln Lys Val Glu Thr Trp 225 230 235 240 Ala Leu Arg His Pro Gly Phe Thr Val Ile Ala Leu Phe Leu Ala His 245 250 255 Ala Ile Gly Thr Ser Ile Thr Gln Lys Gly Ile Ile Phe Ile Leu Leu 260 265 270 Met Leu Val Thr Pro Ser Met Ala Met Arg Cys Val Gly Ile Gly Asn 275 280 285 Arg Asp Phe Val Glu Gly Leu Ser Gly Ala Thr Trp Val Asp Val Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Ile Glu Leu Leu Lys Thr Glu Val Thr Asn Pro Ala Val Leu 325 330 335 Arg Lys Leu Cys Ile Glu Ala Lys Ile Ser Asn Thr Thr Thr Asp Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Ala Thr Leu Val Glu Glu Gln Asp Ala 355 360 365 Asn Phe Val Cys Arg Arg Thr Phe Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Ser Leu Ile Thr Cys Ala Lys Phe Lys 385 390 395 400 Cys Val Thr Lys Leu Glu Gly Lys Ile Val Gln Tyr Glu Asn Leu Lys 405 410 415 Tyr Ser Val Ile Val Thr Val His Thr Gly Asp Gln His Gln Val Gly 420 425 430 Asn Glu Thr Thr Glu His Gly Thr Thr Ala Thr Ile Thr Pro Gln Ala 435 440 445 Pro Thr Ser Glu Ile Gln Leu Thr Asp Tyr Gly Thr Leu Thr Leu Asp 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Thr 465 470 475 480 Met Lys Glu Arg Ser Trp Leu Val His Lys Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Thr Ser Gly Ala Ser Thr Ser Gln Glu Thr Trp Asn 500 505 510 Arg Gln Asp Leu Leu Val Thr Phe Lys Thr Ala His Ala Lys Lys Gln 515 520 525 Glu Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Thr Ser Gly Thr Thr Thr Ile Phe Ala 545 550 555 560 Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Thr Leu Lys Gly 565 570 575 Met Ser Tyr Val Met Cys Thr Gly Ser Phe Lys Leu Glu Lys Glu Val 580 585 590 Ala Glu Thr Gln His Gly Thr Val Leu Val Gln Val Lys Tyr Glu Gly 595 600 605 Thr Asp Ala Pro Cys Lys Ile Pro Phe Ser Thr Gln Asp Glu Lys Gly 610 615 620 Ala Thr Gln Asn Gly Arg Leu Ile Thr Ala Asn Pro Ile Val Thr Asp 625 630 635 640 Lys Glu Lys Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser 645 650 655 Tyr Ile Val Val Gly Ala Gly Glu Lys Ala Leu Lys Leu Ser Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Lys Met Phe Glu Ala Thr Ala Arg Gly 675 680 685 Ala Arg Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Ile Gly Gly Val Phe Thr Ser Met Gly Lys Leu Val His Gln Val Phe 705 710 715 720 Gly Thr Ala Tyr Gly Val Leu Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Gly Ile Gly Ile Leu Leu Thr Trp Leu Gly Leu Asn Ser Arg Asn 740 745 750 Thr Ser Leu Ser Met Met Cys Ile Ala Ala Gly Ile Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Val Ser Trp Lys 770 775 780 Asn Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Ile Thr Asp Asn Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ser Lys 805 810 815 Leu Ala Ser Ala Ile Gln Lys Ala His Glu Glu Asp Ile Cys Gly Ile 820 825 830 Arg Ser Val Thr Arg Leu Glu Asn Leu Met Trp Lys Gln Ile Thr Pro 835 840 845 Glu Leu Asn His Ile Leu Ser Glu Asn Glu Val Lys Leu Thr Ile Met 850 855 860 Thr Gly Asp Ile Lys Gly Ile Met Gln Ala Gly Lys Arg Ser Leu Arg 865 870 875 880 Pro Gln Pro Thr Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala 885 890 895 Lys Met Leu Ser Thr Glu Ser His Asn Gln Thr Phe Leu Ile Asp Gly 900 905 910 Pro Glu Thr Ala Glu Cys Pro Asn Thr Asn Arg Ala Trp Asn Ser Leu 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Lys Glu Lys Gln Asp Val Phe Cys Asp Ser Lys Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Asn Arg Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Ala Leu Asn Asp Thr Trp Lys Ile Glu Lys Ala Ser Phe 980 985 990 Ile Glu Val Lys Asn Cys His Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Asn Leu Ala 1010 1015 1020 Gly Pro Val Ser Gln His Asn Tyr Arg Pro Gly Tyr His Thr Gln 1025 1030 1035 Ile Thr Gly Pro Trp His Leu Gly Lys Leu Glu Met Asp Phe Asp 1040 1045 1050 Phe Cys Asp Gly Thr Thr Val Val Val Thr Glu Asp Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Ile 1070 1075 1080 Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr 1085 1090 1095 Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala Gly 1115 1120 1125 His Gly Gln Val Asp Asn Phe Ser Leu Gly Val Leu Gly Met Ala 1130 1135 1140 Leu Phe Leu Glu Glu Met Leu Arg Thr Arg Val Gly Thr Lys His 1145 1150 1155 Ala Ile Leu Leu Val Ala Val Ser Phe Val Thr Leu Ile Thr Gly 1160 1165 1170 Asn Met Ser Phe Arg Asp Leu Gly Arg Val Met Val Met Val Gly 1175 1180 1185 Ala Thr Met Thr Asp Asp Ile Gly Met Gly Val Thr Tyr Leu Ala 1190 1195 1200 Leu Leu Ala Ala Phe Lys Val Arg Pro Thr Phe Ala Ala Gly Leu 1205 1210 1215 Leu Leu Arg Lys Leu Thr Ser Lys Glu Leu Met Met Thr Thr Ile 1220 1225 1230 Gly Ile Val Leu Leu Ser Gln Ser Thr Leu Pro Glu Thr Ile Leu 1235 1240 1245 Glu Leu Thr Asp Ala Leu Ala Leu Gly Met Met Val Leu Lys Met 1250 1255 1260 Val Arg Asn Met Glu Lys Tyr Gln Leu Ala Val Thr Ile Met Ala 1265 1270 1275 Ile Leu Cys Val Pro Asn Ala Val Ile Leu Gln Asn Ala Trp Lys 1280 1285 1290 Val Ser Cys Thr Ile Leu Ala Val Val Ser Val Ser Pro Leu Phe 1295 1300 1305 Leu Thr Ser Ser Gln Gln Lys Thr Asp Trp Ile Pro Leu Ala Leu 1310 1315 1320 Thr Ile Lys Gly Leu Asn Pro Thr Ala Ile Phe Leu Thr Thr Leu 1325 1330 1335 Ser Arg Thr Ser Lys Lys Arg Ser Trp Pro Leu Asn Glu Ala Ile 1340 1345 1350 Met Ala Val Gly Met Val Ser Ile Leu Ala Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Ile Pro Met Thr Gly Pro Leu Val Ala Gly Gly Leu Leu 1370 1375 1380 Thr Val Cys Tyr Val Leu Thr Gly Arg Ser Ala Asp Leu Glu Leu 1385 1390 1395 Glu Arg Ala Ala Asp Val Lys Trp Glu Asp Gln Ala Glu Ile Ser 1400 1405 1410 Gly Ser Ser Pro Ile Leu Ser Ile Thr Ile Ser Glu Asp Gly Ser 1415 1420 1425 Met Ser Ile Lys Asn Glu Glu Glu Asp Gln Thr Leu Thr Ile Leu 1430 1435 1440 Ile Arg Thr Gly Leu Leu Val Ile Ser Gly Leu Phe Pro Val Ser 1445 1450 1455 Ile Pro Ile Thr Ala Ala Ala Trp Tyr Leu Trp Glu Val Lys Lys 1460 1465 1470 Gln Arg Ala Gly Val Leu Trp Asp Val Pro Ser Pro Pro Pro Met 1475 1480 1485 Gly Lys Ala Glu Leu Glu Asp Gly Ala Tyr Arg Ile Lys Gln Lys 1490 1495 1500 Gly Ile Leu Gly Tyr Ser Gln Ile Gly Ala Gly Val Tyr Lys Glu 1505 1510 1515 Gly Thr Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met His Lys Gly Lys Arg Ile Glu Pro Ser Trp Ala Asp Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Lys Leu Glu Gly Glu 1550 1555 1560 Trp Lys Glu Gly Glu Glu Val Gln Val Leu Ala Leu Glu Pro Gly 1565 1570 1575 Lys Asn Pro Arg Ala Val Gln Thr Lys Pro Gly Leu Phe Lys Thr 1580 1585 1590 Asn Ala Gly Thr Ile Gly Ala Val Ser Leu Asp Phe Ser Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Ile Asp Lys Lys Gly Lys Val Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Arg Ser Gly Ala Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Thr Glu Lys Ser Ile Glu Asp Asn Pro Glu Ile 1640 1645 1650 Glu Asp Asp Ile Phe Arg Lys Arg Arg Leu Thr Ile Met Asp Leu 1655 1660 1665 His Pro Gly Ala Gly Lys Thr Lys Arg Tyr Leu Pro Ala Ile Val 1670 1675 1680 Arg Glu Ala Ile Lys Arg Gly Leu Arg Thr Leu Ile Leu Ala Pro 1685 1690 1695 Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu 1700 1705 1710 Pro Ile Arg Tyr Gln Thr Pro Ala Ile Arg Ala Val His Thr Gly 1715 1720 1725 Arg Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg 1730 1735 1740 Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met 1745 1750 1755 Asp Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly 1760 1765 1770 Tyr Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Gly Ile Phe 1775 1780 1785 Met Thr Ala Thr Pro Pro Gly Ser Arg Asp Pro Phe Pro Gln Ser 1790 1795 1800 Asn Ala Pro Ile Ile Asp Glu Glu Arg Glu Ile Pro Glu Arg Ser 1805 1810 1815 Trp Asn Ser Gly His Glu Trp Val Thr Asp Phe Lys Gly Lys Thr 1820 1825 1830 Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Ala 1835 1840 1845 Cys Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys 1850 1855 1860 Thr Phe Asp Ser Glu Tyr Val Lys Thr Arg Thr Asn Asp Trp Asp 1865 1870 1875 Phe Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys 1880 1885 1890 Ala Glu Arg Val Ile Asp Pro Arg Arg Cys Met Lys Pro Val Ile 1895 1900 1905 Leu Thr Asp Gly Glu Glu Arg Val Ile Leu Ala Gly Pro Met Pro 1910 1915 1920 Val Thr His Ser Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg 1925 1930 1935 Asn Pro Lys Asn Glu Asn Asp Gln Tyr Ile Tyr Met Gly Glu Pro 1940 1945 1950 Leu Glu Asn Asp Glu Asp Cys Ala His Trp Lys Glu Ala Lys Met 1955 1960 1965 Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ser Met 1970 1975 1980 Phe Glu Pro Glu Arg Glu Lys Val Asp Ala Ile Asp Gly Glu Tyr 1985 1990 1995 Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Asp Leu Met Arg 2000 2005 2010 Arg Gly Asp Leu Pro Val Trp Leu Ala Tyr Arg Val Ala Ala Glu 2015 2020 2025 Gly Ile Asn Tyr Ala Asp Arg Arg Trp Cys Phe Asp Gly Val Lys 2030 2035 2040 Asn Asn Gln Ile Leu Glu Glu Asn Val Glu Val Glu Ile Trp Thr 2045 2050 2055 Lys Glu Gly Glu Arg Lys Lys Leu Lys Pro Arg Trp Leu Asp Ala 2060 2065 2070 Arg Ile Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Glu Phe 2075 2080 2085 Ala Ala Gly Arg Lys Ser Leu Thr Leu Asn Leu Ile Thr Glu Met 2090 2095 2100 Gly Arg Leu Pro Thr Phe Met Thr Gln Lys Ala Arg Asp Ala Leu 2105 2110 2115 Asp Asn Leu Ala Val Leu His Thr Ala Glu Ala Gly Gly Arg Ala 2120 2125 2130 Tyr Asn His Ala Leu Ser Glu Leu Pro Glu Thr Leu Glu Thr Leu 2135 2140 2145 Leu Leu Leu Thr Leu Leu Ala Thr Val Thr Gly Gly Ile Phe Leu 2150 2155 2160 Phe Leu Met Ser Ala Arg Gly Ile Gly Lys Met Thr Leu Gly Met 2165 2170 2175 Cys Cys Ile Ile Thr Ala Ser Ile Leu Leu Trp Tyr Ala Gln Ile 2180 2185 2190 Gln Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu 2195 2200 2205 Ile Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln 2210 2215 2220 Asp Asn Gln Leu Thr Tyr Val Val Ile Ala Ile Leu Thr Val Val 2225 2230 2235 Ala Ala Thr Met Ala Asn Glu Met Gly Phe Leu Glu Lys Thr Lys 2240 2245 2250 Lys Asp Leu Gly Leu Gly Ser Ile Ala Thr Gln Gln Pro Glu Ser 2255 2260 2265 Asn Ile Leu Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu 2270 2275 2280 Tyr Ala Val Ala Thr Thr Phe Val Thr Pro Met Leu Arg His Ser 2285 2290 2295 Ile Glu Asn Ser Ser Val Asn Val Ser Leu Thr Ala Ile Ala Asn 2300 2305 2310 Gln Ala Thr Val Leu Met Gly Leu Gly Lys Gly Trp Pro Leu Ser 2315 2320 2325 Lys Met Asp Ile Gly Val Pro Leu Leu Ala Ile Gly Cys Tyr Ser 2330 2335 2340 Gln Val Asn Pro Ile Thr Leu Thr Ala Ala Leu Phe Leu Leu Val 2345 2350 2355 Ala His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr 2360 2365 2370 Arg Glu Ala Gln Lys Arg Ala Ala Ala Gly Ile Met Lys Asn Pro 2375 2380 2385 Thr Val Asp Gly Ile Thr Val Ile Asp Leu Asp Pro Ile Pro Tyr 2390 2395 2400 Asp Pro Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val 2405 2410 2415 Leu Cys Val Thr Gln Val Leu Met Met Arg Thr Thr Trp Ala Leu 2420 2425 2430 Cys Glu Ala Leu Thr Leu Ala Thr Gly Pro Ile Ser Thr Leu Trp 2435 2440 2445 Glu Gly Asn Pro Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser 2450 2455 2460 Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu 2465 2470 2475 Leu Phe Ser Ile Met Lys Asn Thr Thr Asn Thr Arg Arg Gly Thr 2480 2485 2490 Gly Asn Ile Gly Glu Thr Leu Gly Glu Lys Trp Lys Ser Arg Leu 2495 2500 2505 Asn Ala Leu Gly Lys Ser Glu Phe Gln Ile Tyr Lys Lys Ser Gly 2510 2515 2520 Ile Gln Glu Val Asp Arg Thr Leu Ala Lys Glu Gly Ile Lys Arg 2525 2530 2535 Gly Glu Thr Asp His His Ala Val Ser Arg Gly Ser Ala Lys Leu 2540 2545 2550 Arg Trp Phe Val Glu Arg Asn Met Val Thr Pro Glu Gly Lys Val 2555 2560 2565 Val Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Gly 2570 2575 2580 Gly Leu Lys Asn Val Arg Glu Val Lys Gly Leu Thr Lys Gly Gly 2585 2590 2595 Pro Gly His Glu Glu Pro Ile Pro Met Ser Thr Tyr Gly Trp Asn 2600 2605 2610 Leu Val Arg Leu Gln Ser Gly Val Asp Val Phe Phe Ile Pro Pro 2615 2620 2625 Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro 2630 2635 2640 Asn Pro Thr Val Glu Ala Gly Arg Thr Leu Arg Val Leu Asn Leu 2645 2650 2655 Val Glu Asn Trp Leu Asn Asn Asn Thr Gln Phe Cys Ile Lys Val 2660 2665 2670 Leu Asn Pro Tyr Met Pro Ser Val Ile Glu Lys Met Glu Ala Leu 2675 2680 2685 Gln Arg Lys Tyr Gly Gly Ala Leu Val Arg Asn Pro Leu Ser Arg 2690 2695 2700 Asn Ser Thr His Glu Met Tyr Trp Val Ser Asn Ala Ser Gly Asn 2705 2710 2715 Ile Val Ser Ser Val Asn Met Ile Ser Arg Met Leu Ile Asn Arg 2720 2725 2730 Phe Thr Met Arg Tyr Lys Lys Ala Thr Tyr Glu Pro Asp Val Asp 2735 2740 2745 Leu Gly Ser Gly Thr Arg Asn Ile Gly Ile Glu Ser Glu Ile Pro 2750 2755 2760 Asn Leu Asp Ile Ile Gly Lys Arg Ile Glu Lys Ile Lys Gln Glu 2765 2770 2775 His Glu Thr Ser Trp His Tyr Asp Gln Asp His Pro Tyr Lys Thr 2780 2785 2790 Trp Ala Tyr His Gly Ser Tyr Glu Thr Lys Gln Thr Gly Ser Ala 2795 2800 2805 Ser Ser Met Val Asn Gly Val Val Arg Leu Leu Thr Lys Pro Trp 2810 2815 2820 Asp Val Val Pro Met Val Thr Gln Met Ala Met Thr Asp Thr Thr 2825 2830 2835 Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg 2840 2845 2850 Thr Gln Glu Pro Lys Glu Gly Thr Lys Lys Leu Met Lys Ile Thr 2855 2860 2865 Ala Glu Trp Leu Trp Lys Glu Leu Gly Lys Lys Lys Thr Pro Arg 2870 2875 2880 Met Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn Ala 2885 2890 2895 Ala Leu Gly Ala Ile Phe Thr Asp Glu Asn Lys Trp Lys Ser Ala 2900 2905 2910 Arg Glu Ala Val Glu Asp Ser Arg Phe Trp Glu Leu Val Asp Lys 2915 2920 2925 Glu Arg Asn Leu His Leu Glu Gly Lys Cys Glu Thr Cys Val Tyr 2930 2935 2940 Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys 2945 2950 2955 Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg 2960 2965 2970 Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp 2975 2980 2985 Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly Leu 2990 2995 3000 His Lys Leu Gly Tyr Ile Leu Arg Asp Val Ser Lys Lys Glu Gly 3005 3010 3015 Gly Ala Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile 3020 3025 3030 Thr Leu Glu Asp Leu Lys Asn Glu Glu Met Val Thr Asn His Met 3035 3040 3045 Glu Gly Glu His Lys Lys Leu Ala Glu Ala Ile Phe Lys Leu Thr 3050 3055 3060 Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Thr Pro Arg Gly 3065 3070 3075 Thr Val Met Asp Ile Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly 3080 3085 3090 Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala 3095 3100 3105 Gln Leu Ile Arg Gln Met Glu Gly Glu Gly Val Phe Lys Ser Ile 3110 3115 3120 Gln His Leu Thr Ile Thr Glu Glu Ile Ala Val Gln Asn Trp Leu 3125 3130 3135 Ala Arg Val Gly Arg Glu Arg Leu Ser Arg Met Ala Ile Ser Gly 3140 3145 3150 Asp Asp Cys Val Val Lys Pro Leu Asp Asp Arg Phe Ala Ser Ala 3155 3160 3165 Leu Thr Ala Leu Asn Asp Met Gly Lys Ile Arg Lys Asp Ile Gln 3170 3175 3180 Gln Trp Glu Pro Ser Arg Gly Trp Asn Asp Trp Thr Gln Val Pro 3185 3190 3195 Phe Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg 3200 3205 3210 Val Leu Val Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg 3215 3220 3225 Ala Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala 3230 3235 3240 Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe 3245 3250 3255 His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser Ala 3260 3265 3270 Val Pro Ser His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile 3275 3280 3285 His Ala Lys His Glu Trp Met Thr Thr Glu Asp Met Leu Thr Val 3290 3295 3300 Trp Asn Arg Val Trp Ile Gln Glu Asn Pro Trp Met Glu Asp Lys 3305 3310 3315 Thr Pro Val Glu Ser Trp Glu Glu Ile Pro Tyr Leu Gly Lys Arg 3320 3325 3330 Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala 3335 3340 3345 Thr Trp Ala Lys Asn Ile Gln Ala Ala Ile Asn Gln Val Arg Ser 3350 3355 3360 Leu Ile Gly Asn Glu Glu Tyr Thr Asp Tyr Met Pro Ser Met Lys 3365 3370 3375 Arg Phe Arg Arg Glu Glu Glu Glu Ala Gly Val Leu Trp 3380 3385 3390 <210>3 <211>10723 <212>DNA <213>Artificial Sequence <220> <223>Dengue virus serotype 1, MVS <400>3 agttgttagt ctacgtggac cgacaaagac agattctttg agggagctaa gctcaatgta 60 gttctaacag ttttttaatt agagagcaga tctctgatga ataaccaacg gaaaaaggcg 120 aaaaacacgc ctttcaatat gctgaaacgc gagagaaacc gcgtgtcgac tgtgcaacag 180 ctgacaaaga gattctcact tggaatgctg cagggacgag gaccattaaa actgttcatg 240 gccctggtgg cgttccttcg tttcctaaca atcccaccaa cagcagggat attgaagaga 300 tggggaacaa ttaaaaaatc aaaagctatt aatgttttga gagggttcag gaaagagatt 360 ggaaggatgc tgaacatctt gaataggaga cgcagatctg caggcatgat cattatgctg 420 attccaacag tgatggcgtt ccatttaacc acgcgtgggg gagagccgca tatgatagtt 480 agcaagcagg aaagaggaaa gtcacttttg ttcaagacct ctgcaggtgt caacatgtgc 540 accctcattg cgatggattt gggagagttg tgtgaggaca cgatgaccta caaatgcccc 600 cggatcactg aggcggaacc agatgacgtt gactgttggt gcaatgccac ggacacatgg 660 gtgacctatg gaacgtgctc tcaaactggc gaacaccgac gagacaaacg ttccgtcgca 720 ttggccccac acgtggggct tggcctagaa acaagagccg aaacgtggat gtcctctgaa 780 ggtgcttgga aacagataca aaaagtagag acttgggctc tgagacatcc aggattcacg 840 gtgatagccc tttttctagc acatgccata ggaacatcca tcacccagaa agggatcatt 900 ttcattttgc tgatgctggt aacaccatct atggccatgc gatgcgtggg aataggcaac 960 agagacttcg tggaaggact gtcaggagca acatgggtgg atgtggtact ggagcatgga 1020 agttgcgtca ccaccatggc aaaaaacaaa ccaacactgg acattgaact cttgaagacg 1080 gaggtcacaa accctgcagt tctgcgtaaa ttgtgcattg aagctaaaat atcaaacacc 1140 accaccgatt cgagatgtcc aacacaagga gaagccacac tggtggaaga acaagacgcg 1200 aactttgtgt gccgacgaac gttcgtggac agaggctggg gcaatggctg tgggctattc 1260 ggaaaaggta gtctaataac gtgtgccaag tttaagtgtg tgacaaaact agaaggaaag 1320 atagttcaat atgaaaacct aaaatattca gtgatagtca ccgtccacac tggagatcag 1380 caccaggtgg gaaatgagac tacagaacat ggaacaactg caaccataac acctcaagct 1440 cctacgtcgg aaatacagct gaccgactac ggaaccctta cattagattg ttcacctagg 1500 acagggctag attttaacga gatggtgttg ctgacaatga aagaaagatc atggcttgtc 1560 cacaaacaat ggttcctaga cttaccactg ccttggacct ctggggcttc aacatcccaa 1620 gagacttgga acagacaaga tttactggtc acatttaaga cagctcatgc aaagaagcag 1680 gaagtagtcg tactaggatc acaagaagga gcaatgcaca ctgcgctgac tggagcgaca 1740 gaaatccaaa cgtcaggaac gacaacaatt ttcgcaggac acctaaaatg cagactaaaa 1800 atggacaaac taactttaaa agggatgtca tatgtgatgt gcacaggctc attcaagtta 1860 gagaaagaag tggctgagac ccagcatgga actgttctgg tgcaggttaa atatgaagga 1920 acagacgcac catgcaagat tcccttttcg acccaagatg agaaaggagc aacccagaat 1980 gggagattaa taacagccaa ccccatagtc actgacaaag aaaaaccagt caatattgag 2040 gcagaaccac cctttggtga gagctacatc gtggtaggag caggtgaaaa agctttgaaa 2100 ctaagctggt tcaagaaagg aagcagcata gggaaaatgt ttgaagcaac tgcccgagga gcacgaagga tggccattct gggagacacc gcatgggact tcggttctat aggaggagtg ttcacgtcta tgggaaaact ggtacaccag gtttttgga ctgcatatgg agttttgttt agcggagttt cttggaccat gaaaatagga atagggattc tgctgacatg gctaggatta 2340 aattcaagga acacgtccct ttcgatgatg tgcatcgcag ccggcattgt gacactgtat ttgggagtca tggtgcaggc cgatagtggt tgcgttgtga gctggaaaaa caaagaactg aaatgtggca gtgggatttt catcacagac aacgtgcaca catggacaga acaatacaag ttccaaccag aatccccttc aaaactagct tcagctatcc agaaagccca tgaagaggac atttgtggaa tccgctcagt aacaagactg gagaatctga tgtggaaaca aatacacca gaattgaatc acattctatc agaaaatgag gtgaagttaa ctattatgac aggagacatc aaaggaatca tgcaggcagg aaaacgatct ctgcggcctc agcccactga gctgaagtat tcatggaaaa catggggcaa agcaaaaatg ctctctacag agtctcataa ccagaccttt ctcattgatg gccccgaac agcagaatgc cccacaca atagagcttg gattcgttg 2880 gaagttgaag actatggctt tggagtattc accaccaata tattggctaaa attgaaagaa 2940 aaaacaggatg tattctgcga ctcaaactc atgtcagcgg ccaaaaga caacagagcc 3000 gtccatgccg attgggtta ttggatagaa agtgcactca atgacacatg gaagatagag 3060 aaagcctctt tcattgaagt taaaaactgc cactggccaa atcacacac cctctggagc 3120 aatggagtgc tagaagtga gatgatatt ccaagaatc tcgctggacc agtgtctca 3180 cacaactata gaccaggcta ccatacacaa atacaggac catggcatct aggtaagctt 3240 gagatgact tgatttctg tgatgacaca acagtgtag tgactgagga ctgcggaat 3300 agaggaccct ctttgagac aaccactgcc tctggaaac tcatacaga atggtgctgc 3360 cgatcttgca cattaccacc gctaagatac agaggtgagg atggttgctg gtacgggatg 3420 gaatcagac cattgaagga gaagagag aatttggtca actccttggt cacagctgga 3480 catgggcagg tcgacaactt ttcactagga gtcttggga tggcattgtt cctggaggaa 3540 atgcttagga cccgagtagg aacgaaacat gcaatactac tagttgcagt ttcttttgtg 3600 acattgatca cagggaacat gtcctttaga gacctgggaa gagtgatggt tatggtaggc 3660 gccactatga cggatgacat aggtatgggc gtgacttatc ttgccctact agcagccttc 3720 aaagtcagac caacttttgc agctggacta ctcttgagaa agctgacctc caaggaattg 3780 atgatgacta ctataggaat tgtactcctc tcccagagca ccctaccaga gaccattctt 3840 gagttgactg atgcgttagc cttaggcatg atggtcctca aaatggtgag aaatatggaa 3900 aagtatcaat tggcagtgac tatcatggct atcttgtgcg tcccaaacgc agtgatatta 3960 caaaacgcat ggaagtgag ttgcacaata ttggcagtgg tgtccgtttc cccactgttc 4020 ttaacatcct cacagcaaaa aacagattgg ataccattag cattgacgat caaaggtctc 4080 aatccaacag ctattttct aacaaccctc tcaagaacca gcaagaaaag gagctggcca 4140 ttaaatgagg ctatcatggc agtcgggatg gtgagcattt tagccagttc tctcctaaaa 4200 aatgatattc ccatgacagg accattatagtg gctggagggc tcctcactgt gtgctacgtg 4260 ctcactggac gatcggccga tttggaactg gagagagcag ccgatgtcaa atgggaagac 4380. 4380. 4380. 4380. 4380. 4380. 4380. 4380. 4380 atgtcgataa aaaatgaaga ggaagatcaa acactgacca tactcattag aacaggattg ctggtgatct caggactttt tcctgtatca attack cggcagcagc atggtacctg tgggaagtga agaaacaacg ggccggagta ttgtgggatg ttccttcacc cccacccatg ggaaaggctg aactggaaga tggagcctat agaattaagc aaaaagggat tcttggatat tcccagatcg gagccggagt ttacaaagaa ggaacattcc atacaatgtg gcatgtcaca cgtggcgctg ttctaatgca taaaggaaag aggattgaac catcatgggc ggacgtcaag aaagacctaa tatcatatgg aggaggctgg aagttagaag gagaatgga ggaggaga gagtccagg tattggcact gagcctgga aaaaatcca gagccgtcca aacgaaacct ggtcttttca aaaccaacgc cggaacaata ggtgctgtat ctctggactt ttctcctgga 4980. acgtcaggat ctccaattat cgacaaaaaa ggaaaagttg tgggtcttta tggtaatggt gttgttacaa ggagtggagc atatgtgagt gctatagccc agactgaaa aagcattgaa 5040 gaacccag agatcgaga tgacattttc cgaaagagaa gactgaccat catggaccctc 5100 cacccaggag cgggaagac gagagatac cttccggcca tagtcagaga agcttaaaa 5160 cggggtttga gacattaat cttggccccc actagagttg tggcagctga atggaggaa 5220 gcccttagag gacttccaat aagataccag accccagcca tcagagctgt gcacaccggg 5280 cgggagatg tggacctaat gtgtcatgcc accattacca tgaggctgct atcaccagtt 5340 agagtgccaa actacaacct gattatcatg gacgaagccc atttcacaga cccagcaagt 5400 atagcagcta gaggatacat ctcactcga gtggagatgg gtgaggcagc tgggattttt 5460 atgacagcca ctcccccgggg aagcagagac ccattccctc agagcaatgc accaatcata 5520 gatgagaaa gagaatccc tgaacgctcg tggaattccg gatgaatg ggtcacggat 5580 tttaaaggga agactgttg gttcgttcca agtataaaag caggaatga tatagcagct 5640 tgcctgagga aaatggaaa gaagtgata caaccagta ggaagacctt tgattctgag 5700 tatgtcaaga ctagaaccaa tgattgggac ttcgtggtta caactgacat ttcagaaatg 5760 ggtgccaatt tcaaggctga gagggttata gaccccagac gctgcatgaa accagtcata 5820 ctaacagatg gtgaagagcg ggtgattctg gcaggaccta tgccagtgac ccactctagt 5880 gcagcacaaa gaagggag aataggaaga aatccaaaaa atgagaatga ccagtacata 5940 6000 ctcctagata acatcaacac gccagaagga atcattccta gcatgttcga accagagcgt 6060 gaaaaggtgg atgccattga tggcgaatac cgcttgagag gagaagcaag gaaaaccttt 6120 gtagacttaa tgagaagagg agacctacca gtctggttgg cctacagagt ggcagctgaa 6180 ggcatcaact acgcagacag aaggtggtgt tttgatggag tcaagaacaa ccaaatccta 6240 gaaaaacg tggaagttga aatctggaca aaagaagggg aaaggaagaa attgaaaccc 6300 agatggttgg atgctaggat ctattctgac ccactggcgc taaaagaatt taggaattt 6360 gcagccggaa gaaagtctct gaccctgaac ctaatcacag aaatgggtag gctcccaacc 6420 ttcatgactc agaaggcaag agacgcactg gacaacttag cagtgctgca cacggctgag 6480 gcaggtggaa gggcgtacaa ccatgctctc agtgaactgc cggagaccct ggagacattg 6540 cttttactga cacttctggc tacagtcacg ggagggatct ttttattctt gatgagcgca 6600 aggggcatag ggaagatgac cctgggaatg tgctgcataa tcacggctag catcctccta 6660 tggtacgcac aaatacagcc acactggata gcagcttcaa taatactgga gttttttctc 6720 atagttttgc ttattccaga acctgaaaaa cagagaacac cccaagacaa ccaactgacc 6780 tacgttgtca tagccatcct cacagtggtg gccgcaacca tggcaaacga gatgggtttc 6840 ctagaaaaaa cgaagaaaga tctcggattg ggaagcattg caacccagca acccgagagc 6900 aacatcctgg acatagatct acgtcctgca tcagcatgga cgctgtatgc cgtggccaca 6960 acatttgtta caccaatgtt gagacatagc attgaaaatt cctcagtgaa tgtgtcccta 7020 acagctatag ccaaccaagc cacagtgtta atgggtctcg ggaaaggatg gccattgtca 7080 aagatggaca tcggagttcc ccttctcgcc attggatgct actcacaagt caaccccata 7140 actctcacag cagctctttt cttattgta gcacattatg ccatcatagg gccaggactc 7200 caagcaaag caccagaa agctcagaaa agagcagcgg cgggcatcat gaaaaaccca 7260 actgtcgatg gaataacagt gattgaccta gatccaatac cttatgatcc gaagtttgaa 7320 aagcagttgg gatagtaat gctcctagtc ctctgcgtga ctcaagtatt gatgatgagg 7380 actacatggg ctctgtgtga ggctttaacc ttagctaccg ggcccactc cacattgtgg 7440 gaaggaaatc cagggaggtt ttggacact accattgcgg tgtcaatggc tacattttt 7500 agagggagtt acttggccgg agctggactt ctctttcta ttatgagaa cacaccac 7560 acaagaaggg gaactggcaa cataggagag acgcttggag agaatggaa aagccgattg 7620 aacgcattgg gaaaaagtga attccagatc tacaagaaa gtggaatcca ggaagtggat 7680 agaaccttag caaagaagg cattaaaaga ggagaaacgg accacacgc tgtgtcgcga 7740 ggctcagcaa aactgagatg gttcgttgag agaaacatgg tcacaccaga agggaagta 7800 gtggacctcg gttgtggcag aggaggctgg tcatactatt gtggaggact aagaatgta 7860 agagaagtca aaggcctaac aaaaggagga ccaggacacg aagaacccat ccccatgtca 7920 acatatgggt ggaatctagt gcgtcttcaa agtggagttg acgttttctt catcccgcca 7980 gaaaagtgtg acacattatt gtgtgacata ggggagtcat caccaaatcc cacagtggaa 8040 gcaggacgaa cactcagagt ccttaactta gtagaaaatt ggttgaacaa caacactcaa 8100 ttttgcataa aggttctcaa cccatatatg ccctcagtca tagaaaaaat ggaagcacta 8160 caaaggaaat atggaggagc cttagtgagg aatccactct cacgaaactc cacacatgag 8220 atgtactggg tatccaatgc ttccgggaac atagtgtcat cagtgaacat gatttcaagg 8280 atgttgatca acagatttac aatgagatac aagaaagcca cttacgagcc ggatgttgac 8340 ctcggaagcg gaacccgtaa catcgggatt gaaagtgaga taccaaacct agatataatt 8400 gggaaaagaa tagaaaaat aaagcaagag catgaaacat catggcacta tgaccaagac 8460 cacccataca aaacgtgggc ataccatggt agctatgaaa caaaacagac tggatcagca 8520 tcatccatgg tcaacggagt ggtcaggctg ctgacaaaac cttgggacgt cgtccccatg 8580 gtgacacaga tggcaatgac agacacgact ccatttggac aacagcgcgt ttttaaagag 8640 aaagtggaca cgagaaccca agaaccgaa gaggcacga agaactaat gaaaataca 8700 gcagagtggc tttggaaga attaggaag aaaagacac ccaggatgtg caccagagaa 8760 gattcacaa gaaggtgag aagcaatgca gccttgggggg ccatattcac tgatgagaac 8820 aagtggaagt cggcacgtga ggctgttgaa gatagtaggt tttgggagct ggttgacaag 8880 gaaaggaatc tccatcttga aggaaagtgt gaaacatgtg tgtacaacat gatgggaaaa 8940 aggagaga agctagggga attcggcaag gcaaaggca gcagagccat atggtacatg 9000 tggcttggag cacgcttctt agagtttga gccctaggat tctttaatga agatcactgg 9060 ttctccagag agaactccct gagtggagtg gaaggagaag ggctgcacaa gctaggttac 9120 attctaagag acgtgagcaa gaagaggga ggagcaatgt atgccgatga caccgcagga 9180 tgggatacaa gatcacacact agaagaccta aaaaatgaag aaatggtaac aaaccacatg 9240 gaaggagaac acaagaact agccgaggcc atttcaac taacgtacca aaacaggtg 9300 gtgcgtgtgc aaagaccaac accaagaggc acagtaatgg acatcatatc gagagagac 9360 haaagaggta gtggacaagt tggcacctat ggactcaata ctttcaccaa tatggaagcc 9420 caactaatca gagatgga gggagaagga gtctttaaaa gcattcagca cctaacaatc 9480 acagaagaaa tcgctgtgca aaactggtta gcaagagtgg ggcgcgaaag gttatcaaga 9540 atggccatca gtggagatga ttgtgttgtg aaacctttag atgacaggtt cgcaagcgct 9600 ttaacagctc taaatgacat gggaagatt aggaagaca tacacaatg ggaaccttca 9660 agaggatgga atgattggac acaagtgccc ttctgttcac accattcca tgagttaatc 9720 atgaaagacg gtcgcgtact cgttgttcca tgtagaaacc aagatgaact gattggcaga 9780 gcccgaatct cccaaggagc agggtggtct ttgcgggaga cggcctgtttt ggggaagtct 9840 tacgcccaaa tgtggagctt gatgtacttc cacagacgcg acctcaggct ggcggcaaat 9900 gctatttgct cggcagtacc atcacattgg gttccacaa gtcgacaac ctggtccata 9960 catgctaaac atgaatggat vakaacggaa gatatgctga cagtctggaa cagggtgtgg 10020 attcaagaaa acccatggat ggaagacaaa actccagtgg aatcatggga ggaaatccca 10080 tacttgggga aaagagaaga ccaatggtgc ggctcattga ttgggttaac aagcagggcc 10140 acctgggcaa agaacatcca agcagcaata aatcaagtta gatcccttat aggcaatgaa 10200 gaatacacag attacatgcc atccatgaaa agattcagaa gagaagagga agaagcagga 10260 gttctgtggt agaaagcaaa actaacatga aacaaggcta gaagtcaggt cggattaagc 10320 catagtacgg aaaaaactat gctacctgtg agccccgtcc aaggacgtta aaagaagtca 10380 ggccatcata aatgccatag cttgagtaaa ctatgcagcc tgtagctcca cctgagaagg 10440 tgtaaaaaat ccgggaggcc acaaaccatg gaagctgtac gcatggcgta gtggactagc 10500 ggttagagga gacccctccc ttacaaatcg cagcaacaat gggggcccaa ggcgagatga 10560 agctgtagtc tcgctggaag gactagaggt tagaggagac ccccccgaaa caaaaaacag 10620 catattgacg ctgggaaaga ccagagatcc tgctgtctcc tcagcatcat tccaggcaca 10680 gaacgccaga aaatggaatg gtgctgttga atcaacaggt tct 10723 <210>4 <211>3391 <212>PRT <213>Artificial sequence <220> <223>Dengue virus serotype 1, MVS <400>4 Met Asn Asn Gln Arg Lys Lys Ala Lys Asn Thr Pro Phe Asn Met Leu 1 5 10 15 Lys Arg Glu Arg Asn Arg Val Ser Thr Val Gln Gln Leu Thr Lys Arg 20 25 30 Phe Ser Leu Gly Met Leu Gln Gly Arg Gly Pro Leu Lys Leu Phe Met 35 40 45 Ala Leu Val Ala Phe Leu Arg Phe Leu Thr Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Lys Arg Trp Gly Thr Ile Lys Lys Ser Lys Ala Ile Asn Val 65 70 75 80 Leu Arg Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn 85 90 95 Arg Arg Arg Arg Ser Ala Gly Met Ile Ile Met Leu Ile Pro Thr Val 100 105 110 Met Ala Phe His Leu Thr Thr Arg Gly Gly Glu Pro His Met Ile Val 115 120 125 Ser Lys Gln Glu Arg Gly Lys Ser Leu Leu Phe Lys Thr Ser Ala Gly 130 135 140 Val Asn Met Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Met Thr Tyr Lys Cys Pro Arg Ile Thr Glu Ala Glu Pro Asp 165 170 175 Asp Val Asp Cys Trp Cys Asn Ala Thr Asp Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Ser Gln Thr Gly Glu His Arg Arg Asp Lys Arg Ser Val Ala 195 200 205 Leu Ala Pro His Val Gly Leu Gly Leu Glu Thr Arg Ala Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys Gln Ile Gln Lys Val Glu Thr Trp 225 230 235 240 Ala Leu Arg His Pro Gly Phe Thr Val Ile Ala Leu Phe Leu Ala His 245 250 255 Ala Ile Gly Thr Ser Ile Thr Gln Lys Gly Ile Ile Phe Ile Leu Leu 260 265 270 Met Leu Val Thr Pro Ser Met Ala Met Arg Cys Val Gly Ile Gly Asn 275 280 285 Arg Asp Phe Val Glu Gly Leu Ser Gly Ala Thr Trp Val Asp Val Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Ile Glu Leu Leu Lys Thr Glu Val Thr Asn Pro Ala Val Leu 325 330 335 Arg Lys Leu Cys Ile Glu Ala Lys Ile Ser Asn Thr Thr Thr Asp Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Ala Thr Leu Val Glu Glu Gln Asp Ala 355 360 365 Asn Phe Val Cys Arg Arg Thr Phe Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Ser Leu Ile Thr Cys Ala Lys Phe Lys 385 390 395 400 Cys Val Thr Lys Leu Glu Gly Lys Ile Val Gln Tyr Glu Asn Leu Lys 405 410 415 Tyr Ser Val Ile Val Thr Val His Thr Gly Asp Gln His Gln Val Gly 420 425 430 Asn Glu Thr Thr Glu His Gly Thr Thr Ala Thr Ile Thr Pro Gln Ala 435 440 445 Pro Thr Ser Glu Ile Gln Leu Thr Asp Tyr Gly Thr Leu Thr Leu Asp 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Thr 465 470 475 480 Met Lys Glu Arg Ser Trp Leu Val His Lys Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Thr Ser Gly Ala Ser Thr Ser Gln Glu Thr Trp Asn 500 505 510 Arg Gln Asp Leu Leu Val Thr Phe Lys Thr Ala His Ala Lys Lys Gln 515 520 525 Glu Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Thr Ser Gly Thr Thr Thr Ile Phe Ala 545 550 555 560 Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Thr Leu Lys Gly 565 570 575 Met Ser Tyr Val Met Cys Thr Gly Ser Phe Lys Leu Glu Lys Glu Val 580 585 590 Ala Glu Thr Gln His Gly Thr Val Leu Val Gln Val Lys Tyr Glu Gly 595 600 605 Thr Asp Ala Pro Cys Lys Ile Pro Phe Ser Thr Gln Asp Glu Lys Gly 610 615 620 Ala Thr Gln Asn Gly Arg Leu Ile Thr Ala Asn Pro Ile Val Thr Asp 625 630 635 640 Lys Glu Lys Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser 645 650 655 Tyr Ile Val Val Gly Ala Gly Glu Lys Ala Leu Lys Leu Ser Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Lys Met Phe Glu Ala Thr Ala Arg Gly 675 680 685 Ala Arg Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Ile Gly Gly Val Phe Thr Ser Met Gly Lys Leu Val His Gln Val Phe 705 710 715 720 Gly Thr Ala Tyr Gly Val Leu Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Gly Ile Gly Ile Leu Leu Thr Trp Leu Gly Leu Asn Ser Arg Asn 740 745 750 Thr Ser Leu Ser Met Met Cys Ile Ala Ala Gly Ile Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Val Ser Trp Lys 770 775 780 Asn Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Ile Thr Asp Asn Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ser Lys 805 810 815 Leu Ala Ser Ala Ile Gln Lys Ala His Glu Glu Asp Ile Cys Gly Ile 820 825 830 Arg Ser Val Thr Arg Leu Glu Asn Leu Met Trp Lys Gln Ile Thr Pro 835 840 845 Glu Leu Asn His Ile Leu Ser Glu Asn Glu Val Lys Leu Thr Ile Met 850 855 860 Thr Gly Asp Ile Lys Gly Ile Met Gln Ala Gly Lys Arg Ser Leu Arg 865 870 875 880 Pro Gln Pro Thr Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala 885 890 895 Lys Met Leu Ser Thr Glu Ser His Asn Gln Thr Phe Leu Ile Asp Gly 900 905 910 Pro Glu Thr Ala Glu Cys Pro Asn Thr Asn Arg Ala Trp Asn Ser Leu 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Lys Glu Lys Gln Asp Val Phe Cys Asp Ser Lys Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Asn Arg Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Ala Leu Asn Asp Thr Trp Lys Ile Glu Lys Ala Ser Phe 980 985 990 Ile Glu Val Lys Asn Cys His Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Asn Leu Ala 1010 1015 1020 Gly Pro Val Ser Gln His Asn Tyr Arg Pro Gly Tyr His Thr Gln 1025 1030 1035 Ile Thr Gly Pro Trp His Leu Gly Lys Leu Glu Met Asp Phe Asp 1040 1045 1050 Phe Cys Asp Gly Thr Thr Val Val Val Thr Glu Asp Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Ile 1070 1075 1080 Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr 1085 1090 1095 Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala Gly 1115 1120 1125 His Gly Gln Val Asp Asn Phe Ser Leu Gly Val Leu Gly Met Ala 1130 1135 1140 Leu Phe Leu Glu Glu Met Leu Arg Thr Arg Val Gly Thr Lys His 1145 1150 1155 Ala Ile Leu Leu Val Ala Val Ser Phe Val Thr Leu Ile Thr Gly 1160 1165 1170 Asn Met Ser Phe Arg Asp Leu Gly Arg Val Met Val Met Val Gly 1175 1180 1185 Ala Thr Met Thr Asp Asp Ile Gly Met Gly Val Thr Tyr Leu Ala 1190 1195 1200 Leu Leu Ala Ala Phe Lys Val Arg Pro Thr Phe Ala Ala Gly Leu 1205 1210 1215 Leu Leu Arg Lys Leu Thr Ser Lys Glu Leu Met Met Thr Thr Ile 1220 1225 1230 Gly Ile Val Leu Leu Ser Gln Ser Thr Leu Pro Glu Thr Ile Leu 1235 1240 1245 Glu Leu Thr Asp Ala Leu Ala Leu Gly Met Met Val Leu Lys Met 1250 1255 1260 Val Arg Asn Met Glu Lys Tyr Gln Leu Ala Val Thr Ile Met Ala 1265 1270 1275 Ile Leu Cys Val Pro Asn Ala Val Ile Leu Gln Asn Ala Trp Lys 1280 1285 1290 Val Ser Cys Thr Ile Leu Ala Val Val Ser Val Ser Pro Leu Phe 1295 1300 1305 Leu Thr Ser Ser Gln Gln Lys Thr Asp Trp Ile Pro Leu Ala Leu 1310 1315 1320 Thr Ile Lys Gly Leu Asn Pro Thr Ala Ile Phe Leu Thr Thr Leu 1325 1330 1335 Ser Arg Thr Ser Lys Lys Arg Ser Trp Pro Leu Asn Glu Ala Ile 1340 1345 1350 Met Ala Val Gly Met Val Ser Ile Leu Ala Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Ile Pro Met Thr Gly Pro Leu Val Ala Gly Gly Leu Leu 1370 1375 1380 Thr Val Cys Tyr Val Leu Thr Gly Arg Ser Ala Asp Leu Glu Leu 1385 1390 1395 Glu Arg Ala Ala Asp Val Lys Trp Glu Asp Gln Ala Glu Ile Ser 1400 1405 1410 Gly Ser Ser Pro Ile Leu Ser Ile Thr Ile Ser Glu Asp Gly Ser 1415 1420 1425 Met Ser Ile Lys Asn Glu Glu Glu Asp Gln Thr Leu Thr Ile Leu 1430 1435 1440 Ile Arg Thr Gly Leu Leu Val Ile Ser Gly Leu Phe Pro Val Ser 1445 1450 1455 Ile Pro Ile Thr Ala Ala Ala Trp Tyr Leu Trp Glu Val Lys Lys 1460 1465 1470 Gln Arg Ala Gly Val Leu Trp Asp Val Pro Ser Pro Pro Pro Met 1475 1480 1485 Gly Lys Ala Glu Leu Glu Asp Gly Ala Tyr Arg Ile Lys Gln Lys 1490 1495 1500 Gly Ile Leu Gly Tyr Ser Gln Ile Gly Ala Gly Val Tyr Lys Glu 1505 1510 1515 Gly Thr Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met His Lys Gly Lys Arg Ile Glu Pro Ser Trp Ala Asp Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Lys Leu Glu Gly Glu 1550 1555 1560 Trp Lys Glu Gly Glu Glu Val Gln Val Leu Ala Leu Glu Pro Gly 1565 1570 1575 Lys Asn Pro Arg Ala Val Gln Thr Lys Pro Gly Leu Phe Lys Thr 1580 1585 1590 Asn Ala Gly Thr Ile Gly Ala Val Ser Leu Asp Phe Ser Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Ile Asp Lys Lys Gly Lys Val Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Arg Ser Gly Ala Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Thr Glu Lys Ser Ile Glu Asp Asn Pro Glu Ile 1640 1645 1650 Glu Asp Asp Ile Phe Arg Lys Arg Arg Leu Thr Ile Met Asp Leu 1655 1660 1665 His Pro Gly Ala Gly Lys Thr Lys Arg Tyr Leu Pro Ala Ile Val 1670 1675 1680 Arg Glu Ala Ile Lys Arg Gly Leu Arg Thr Leu Ile Leu Ala Pro 1685 1690 1695 Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu 1700 1705 1710 Pro Ile Arg Tyr Gln Thr Pro Ala Ile Arg Ala Val His Thr Gly 1715 1720 1725 Arg Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg 1730 1735 1740 Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met 1745 1750 1755 Asp Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly 1760 1765 1770 Tyr Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Gly Ile Phe 1775 1780 1785 Met Thr Ala Thr Pro Pro Gly Ser Arg Asp Pro Phe Pro Gln Ser 1790 1795 1800 Asn Ala Pro Ile Ile Asp Glu Glu Arg Glu Ile Pro Glu Arg Ser 1805 1810 1815 Trp Asn Ser Gly His Glu Trp Val Thr Asp Phe Lys Gly Lys Thr 1820 1825 1830 Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Ala 1835 1840 1845 Cys Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys 1850 1855 1860 Thr Phe Asp Ser Glu Tyr Val Lys Thr Arg Thr Asn Asp Trp Asp 1865 1870 1875 Phe Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys 1880 1885 1890 Ala Glu Arg Val Ile Asp Pro Arg Arg Cys Met Lys Pro Val Ile 1895 1900 1905 Leu Thr Asp Gly Glu Glu Arg Val Ile Leu Ala Gly Pro Met Pro 1910 1915 1920 Val Thr His Ser Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg 1925 1930 1935 Asn Pro Lys Asn Glu Asn Asp Gln Tyr Ile Tyr Met Gly Glu Pro 1940 1945 1950 Leu Glu Asn Asp Glu Asp Cys Ala His Trp Lys Glu Ala Lys Met 1955 1960 1965 Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ser Met 1970 1975 1980 Phe Glu Pro Glu Arg Glu Lys Val Asp Ala Ile Asp Gly Glu Tyr 1985 1990 1995 Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Asp Leu Met Arg 2000 2005 2010 Arg Gly Asp Leu Pro Val Trp Leu Ala Tyr Arg Val Ala Ala Glu 2015 2020 2025 Gly Ile Asn Tyr Ala Asp Arg Arg Trp Cys Phe Asp Gly Val Lys 2030 2035 2040 Asn Asn Gln Ile Leu Glu Glu Asn Val Glu Val Glu Ile Trp Thr 2045 2050 2055 Lys Glu Gly Glu Arg Lys Lys Leu Lys Pro Arg Trp Leu Asp Ala 2060 2065 2070 Arg Ile Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Glu Phe 2075 2080 2085 Ala Ala Gly Arg Lys Ser Leu Thr Leu Asn Leu Ile Thr Glu Met 2090 2095 2100 Gly Arg Leu Pro Thr Phe Met Thr Gln Lys Ala Arg Asp Ala Leu 2105 2110 2115 Asp Asn Leu Ala Val Leu His Thr Ala Glu Ala Gly Gly Arg Ala 2120 2125 2130 Tyr Asn His Ala Leu Ser Glu Leu Pro Glu Thr Leu Glu Thr Leu 2135 2140 2145 Leu Leu Leu Thr Leu Leu Ala Thr Val Thr Gly Gly Ile Phe Leu 2150 2155 2160 Phe Leu Met Ser Ala Arg Gly Ile Gly Lys Met Thr Leu Gly Met 2165 2170 2175 Cys Cys Ile Ile Thr Ala Ser Ile Leu Leu Trp Tyr Ala Gln Ile 2180 2185 2190 Gln Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu 2195 2200 2205 Ile Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln 2210 2215 2220 Asp Asn Gln Leu Thr Tyr Val Val Ile Ala Ile Leu Thr Val Val 2225 2230 2235 Ala Ala Thr Met Ala Asn Glu Met Gly Phe Leu Glu Lys Thr Lys 2240 2245 2250 Lys Asp Leu Gly Leu Gly Ser Ile Ala Thr Gln Gln Pro Glu Ser 2255 2260 2265 Asn Ile Leu Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu 2270 2275 2280 Tyr Ala Val Ala Thr Thr Phe Val Thr Pro Met Leu Arg His Ser 2285 2290 2295 Ile Glu Asn Ser Ser Val Asn Val Ser Leu Thr Ala Ile Ala Asn 2300 2305 2310 Gln Ala Thr Val Leu Met Gly Leu Gly Lys Gly Trp Pro Leu Ser 2315 2320 2325 Lys Met Asp Ile Gly Val Pro Leu Leu Ala Ile Gly Cys Tyr Ser 2330 2335 2340 Gln Val Asn Pro Ile Thr Leu Thr Ala Ala Leu Phe Leu Leu Val 2345 2350 2355 Ala His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr 2360 2365 2370 Arg Glu Ala Gln Lys Arg Ala Ala Ala Gly Ile Met Lys Asn Pro 2375 2380 2385 Thr Val Asp Gly Ile Thr Val Ile Asp Leu Asp Pro Ile Pro Tyr 2390 2395 2400 Asp Pro Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val 2405 2410 2415 Leu Cys Val Thr Gln Val Leu Met Met Arg Thr Thr Trp Ala Leu 2420 2425 2430 Cys Glu Ala Leu Thr Leu Ala Thr Gly Pro Ile Ser Thr Leu Trp 2435 2440 2445 Glu Gly Asn Pro Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser 2450 2455 2460 Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu 2465 2470 2475 Leu Phe Ser Ile Met Lys Asn Thr Thr Asn Thr Arg Arg Gly Thr 2480 2485 2490 Gly Asn Ile Gly Glu Thr Leu Gly Glu Lys Trp Lys Ser Arg Leu 2495 2500 2505 Asn Ala Leu Gly Lys Ser Glu Phe Gln Ile Tyr Lys Lys Ser Gly 2510 2515 2520 Ile Gln Glu Val Asp Arg Thr Leu Ala Lys Glu Gly Ile Lys Arg 2525 2530 2535 Gly Glu Thr Asp His His Ala Val Ser Arg Gly Ser Ala Lys Leu 2540 2545 2550 Arg Trp Phe Val Glu Arg Asn Met Val Thr Pro Glu Gly Lys Val 2555 2560 2565 Val Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Gly 2570 2575 2580 Gly Leu Lys Asn Val Arg Glu Val Lys Gly Leu Thr Lys Gly Gly 2585 2590 2595 Pro Gly His Glu Glu Pro Ile Pro Met Ser Thr Tyr Gly Trp Asn 2600 2605 2610 Leu Val Arg Leu Gln Ser Gly Val Asp Val Phe Phe Ile Pro Pro 2615 2620 2625 Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro 2630 2635 2640 Asn Pro Thr Val Glu Ala Gly Arg Thr Leu Arg Val Leu Asn Leu 2645 2650 2655 Val Glu Asn Trp Leu Asn Asn Asn Thr Gln Phe Cys Ile Lys Val 2660 2665 2670 Leu Asn Pro Tyr Met Pro Ser Val Ile Glu Lys Met Glu Ala Leu 2675 2680 2685 Gln Arg Lys Tyr Gly Gly Ala Leu Val Arg Asn Pro Leu Ser Arg 2690 2695 2700 Asn Ser Thr His Glu Met Tyr Trp Val Ser Asn Ala Ser Gly Asn 2705 2710 2715 Ile Val Ser Ser Val Asn Met Ile Ser Arg Met Leu Ile Asn Arg 2720 2725 2730 Phe Thr Met Arg Tyr Lys Lys Ala Thr Tyr Glu Pro Asp Val Asp 2735 2740 2745 Leu Gly Ser Gly Thr Arg Asn Ile Gly Ile Glu Ser Glu Ile Pro 2750 2755 2760 Asn Leu Asp Ile Ile Gly Lys Arg Ile Glu Lys Ile Lys Gln Glu 2765 2770 2775 His Glu Thr Ser Trp His Tyr Asp Gln Asp His Pro Tyr Lys Thr 2780 2785 2790 Trp Ala Tyr His Gly Ser Tyr Glu Thr Lys Gln Thr Gly Ser Ala 2795 2800 2805 Ser Ser Met Val Asn Gly Val Val Arg Leu Leu Thr Lys Pro Trp 2810 2815 2820 Asp Val Val Pro Met Val Thr Gln Met Ala Met Thr Asp Thr Thr 2825 2830 2835 Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg 2840 2845 2850 Thr Gln Glu Pro Lys Glu Gly Thr Lys Lys Leu Met Lys Ile Thr 2855 2860 2865 Ala Glu Trp Leu Trp Lys Glu Leu Gly Lys Lys Lys Thr Pro Arg 2870 2875 2880 Met Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn Ala 2885 2890 2895 Ala Leu Gly Ala Ile Phe Thr Asp Glu Asn Lys Trp Lys Ser Ala 2900 2905 2910 Arg Glu Ala Val Glu Asp Ser Arg Phe Trp Glu Leu Val Asp Lys 2915 2920 2925 Glu Arg Asn Leu His Leu Glu Gly Lys Cys Glu Thr Cys Val Tyr 2930 2935 2940 Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys 2945 2950 2955 Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg 2960 2965 2970 Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp 2975 2980 2985 Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly Leu 2990 2995 3000 His Lys Leu Gly Tyr Ile Leu Arg Asp Val Ser Lys Lys Glu Gly 3005 3010 3015 Gly Ala Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile 3020 3025 3030 Thr Leu Glu Asp Leu Lys Asn Glu Glu Met Val Thr Asn His Met 3035 3040 3045 Glu Gly Glu His Lys Lys Leu Ala Glu Ala Ile Phe Lys Leu Thr 3050 3055 3060 Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Thr Pro Arg Gly 3065 3070 3075 Thr Val Met Asp Ile Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly 3080 3085 3090 Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala 3095 3100 3105 Gln Leu Ile Arg Gln Met Glu Gly Glu Gly Val Phe Lys Ser Ile 3110 3115 3120 Gln His Leu Thr Ile Thr Glu Glu Ile Ala Val Gln Asn Trp Leu 3125 3130 3135 Ala Arg Val Gly Arg Glu Arg Leu Ser Arg Met Ala Ile Ser Gly 3140 3145 3150 Asp Asp Cys Val Val Lys Pro Leu Asp Asp Arg Phe Ala Ser Ala 3155 3160 3165 Leu Thr Ala Leu Asn Asp Met Gly Lys Ile Arg Lys Asp Ile Gln 3170 3175 3180 Gln Trp Glu Pro Ser Arg Gly Trp Asn Asp Trp Thr Gln Val Pro 3185 3190 3195 Phe Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg 3200 3205 3210 Val Leu Val Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg 3215 3220 3225 Ala Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala 3230 3235 3240 Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe 3245 3250 3255 His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser Ala 3260 3265 3270 Val Pro Ser His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile 3275 3280 3285 His Ala Lys His Glu Trp Met Thr Thr Glu Asp Met Leu Thr Val 3290 3295 3300 Trp Asn Arg Val Trp Ile Gln Glu Asn Pro Trp Met Glu Asp Lys 3305 3310 3315 Thr Pro Val Glu Ser Trp Glu Glu Ile Pro Tyr Leu Gly Lys Arg 3320 3325 3330 Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala 3335 3340 3345 Thr Trp Ala Lys Asn Ile Gln Ala Ala Ile Asn Gln Val Arg Ser 3350 3355 3360 Leu Ile Gly Asn Glu Glu Tyr Thr Asp Tyr Met Pro Ser Met Lys 3365 3370 3375 Arg Phe Arg Arg Glu Glu Glu Glu Ala Gly Val Leu Trp 3380 3385 3390 <210> 5 <211> 10723 <212> DNA <213> Artificial sequence <220> <223> Dengue virus serotype 1, WVS <220> <221> misc_feature <222> (2384)..(2384) <223> n is cytosine or guanine <400> 5 agttgttagt ctacgtggac cgacaaagac agattctttg agggagctaa gctcaatgta 60 gttctaacag ttttttaatt agagagcaga tctctgatga ataaccaacg gaaaaaggcg 120 aaaaacacgc ctttcaatat gctgaaacgc gagagaaacc gcgtgtcgac tgtgcaacag 180 ctgacaaaga gattctcact tggaatgctg cagggacgag gaccattaaa actgttcatg 240 gccctggtgg cgttccttcg tttcctaaca atcccaccaa cagcagggat attgaagaga 300 tggggaacaa ttaaaaaatc aaaagctatt aatgttttga gagggttcag gaaagagatt 360 ggaaggatgc tgaacatctt gaataggaga cgcagatctg caggcatgat cattatgctg 420 attccaacag tgatggcgtt ccatttaacc acgcgtgggg gagagccgca tatgatagtt 480 agcaagcagg aaagaggaaa gtcacttttg ttcaagacct ctgcaggtgt caacatgtgc 540 accctcattg cgatggattt gggagagttg tgtgaggaca cgatgaccta caaatgcccc 600 cggatcactg aggcggaacc agatgacgtt gactgttggt gcaatgccac ggacacatgg 660 gtgacctatg gaacgtgctc tcaaactggc gaacaccgac gagacaaacg ttccgtcgca 720 ttggccccac acgtggggct tggcctagaa acaagagccg aaacgtggat gtcctctgaa 780 ggtgcttgga aacagataca aaaagtagag acttgggctc tgagacatcc aggattcacg 840 gtgatagccc tttttctagc acatgccata ggaacatcca tcacccagaa agggatcatt 900 ttcattttgc tgatgctggt aacaccatct atggccatgc gatgcgtggg aataggcaac 960 agagacttcg tggaaggact gtcaggagca acatgggtgg atgtggtact ggagcatgga 1020 agttgcgtca ccaccatggc aaaaaacaaa ccaacactgg acattgaact cttgaagacg 1080 gaggtcacaa accctgcagt tctgcgtaaa ttgtgcattg aagctaaaat atcaaacacc 1140 accaccgatt cgagatgtcc aacacaagga gaagccacac tggtggaaga acaagacgcg 1200 aactttgtgt gccgacgaac gttcgtggac agaggctggg gcaatggctg tgggctattc 1260 ggaaaaggta gtctaataac gtgtgccaag tttaagtgtg tgacaaaact agaaggaaag 1320 atagttcaat atgaaaacct aaaatattca gtgatagtca ccgtccacac tggagatcag 1380 caccaggtgg gaaatgagac tacagaacat ggaacaactg caaccataac acctcaagct 1440 cctacgtcgg aaatacagct gaccgactac ggaaccctta cattagattg ttcacctagg 1500 acagggctag attttaacga gatggtgttg ctgacaatga aagaaagatc atggcttgtc 1560 cacaaacaat ggttcctaga cttaccactg ccttggacct ctggggcttc aacatcccaa 1620 gagacttgga acagacaaga tttactggtc acatttaaga cagctcatgc aaagaagcag gaagtagtcg tactaggatc acaagaagga gcaatgcaca ctgcgctgac tggagcgaca gaatccaa cgtcaggac gacacaatt ttcgcaggac acctaaaatg cagactaaaa atggacaaac taactttaaa agggatgtca tatgtgatgt gcacaggctc attcaagtta gagaaagag tggctgagac ccagcatgga actgttctgg tgcaggttaa atatgaagga acagacgcac catgcagat tcccttttcg acccaagatg agaaaggagc aacccagat gggagata taacagccaa ccccatagtc actgacaaag aaaaaccagt caatattgag gcagaaccac cctttggtga gagctacatc gtggtaggag caggtgaaaa agctttgaaa ctaagctggt tcaagaaagg aagcagcata gggaaaatgt ttgaagcaac tgcccgagga gcacgaagga tggccattct gggagacacc gcatgggact tcggttctat aggaggagtg ttcacgtcta tgggaaaact ggtacaccag gtttttgga ctgcatatgg agttttgttt agcggagttt cttggaccat gaaaatagga atagggattc tgctgacatg gctaggatta 2340 aattcagga acacgtccct ttcgatg tgcatcgcag ccgncattgt jagactgtat 2400 ttgggagtca tggtgcaggc cgatagtggt tgcgttgtga gctggaaaaa caagaactg 2460 aaatgtggca gtgggattt catcacagac aacgtgcaca catggacaga acatacaag 2520 ttccaaccag aatccccttc aaaactagct tcagctatcc agaagccca tgaagaggac 2580 atttgtggaa tccgctcagt aacagactg gagaatctga tgtggaaca aaacacca 2640 gattgaatc acattctatc agaaaatgag gtgaagttaa ctattatgac aggagacatc 2700 aaaggaatca tgcaggcagg aaacgatct ctgcggccctc agcccactga gctgaagtat 2760 tcatggaaaa catggggcaa agcaaaatg ctctctacag agtctcataa ccagaccttt 2820 ctcattgatg gccccgaac agcagaatgc cccacaca atagagcttg gattcgttg 2880 gaagttgaag actatggctt tggagtattc accaccaata tattggctaaa attgaaagaa 2940 aaaacaggatg tattctgcga ctcaaactc atgtcagcgg ccaaaaga caacagagcc 3000 gtccatgccg attgggtta ttggatagaa agtgcactca atgacacatg gaagatagag 3060 aaagcctctt tcattgaagt taaaaactgc cactggccaa atcacacac cctctggagc 3120 aatggagtgc tagaagtga gatgatatt ccaagaatc tcgctggacc agtgtctca 3180 cacaactata gaccaggcta ccatacacaa atacaggac catggcatct aggtaagctt 3240 gagatgact tgatttctg tgatgacaca acagtgtag tgactgagga ctgcggaat 3300 agaggaccct ctttgagac aaccactgcc tctggaaac tcatacaga atggtgctgc 3360 cgatcttgca cattaccacc gctaagatac agaggtgagg atggttgctg gtacgggatg 3420 gaatcagac cattgaagga gaagagag aatttggtca actccttggt cacagctgga 3480 catgggcagg tcgacaactt ttcactagga gtcttggga tggcattgtt cctggaggaa 3540 atgcttagga cccgagtagg acgaaacat gcaatactac tagttgcagt ttctttgtg 3600 acattgatca cagggaacat gtcctttaga gacctgggaa gagtgatggt tatggtaggc 3660 gccactatga cggatgacat aggtatgggc gtgacttatc ttgcctact agcagccttc 3720 aaagtcagac caacttgc agctggacta ctcttgagaa agctgaccctc caggaattg 3780 atgatgacta ctataggaat tgtactcctc tcccagagca ccctaccaga gaccattctt 3840 gagttgactg atgcgttagc cttaggcatg atggtcctca aaatggtgag aaatatggaa 3900 aagtatcaat tggcagtgac tatcatggct atcttgtgcg tcccaaacgc agtgatatta 3960 caaaacgcat ggaaagtgag ttgcacaata ttggcagtgg tgtccgtttc cccactgttc 4020 ttaacatcct cacagcaaaa aacagattgg ataccattag cattgacgat caaaggtctc 4080 aatccaacag ctatttttct aacaaccctc tcaagaacca gcaagaaaag gagctggcca 4140 ttaaatgagg ctatcatggc agtcgggatg gtgagcattt tagccagttc tctcctaaaa 4200 aatgatattc ccatgacagg accattagtg gctggagggc tcctcactgt gtgctacgtg 4260 ctcactggac gatcggccga tttggaactg gagagagcag ccgatgtcaa atgggaagac 4320 caggcagaga tatcaggaag cagtccaatc ctgtcaataa caatatcaga agatggtagc 4380 atgtcgataa aaaatgaaga ggaagatcaa acactgacca tactcattag aacaggattg 4440 ctggtgatct caggactttt tcctgtatca ataccaatca cggcagcagc atggtacctg 4500 tgggaagtga agaaacaacg ggccggagta ttgtgggatg ttccttcacc cccacccatg 4560. ggaaaggctg aactggaaga tggagcctat agaattaagc aaaaagggat tcttggatat tcccagatcg gagccggagt ttacaaagaa ggaacattcc atacaatgtg gcatgtcaca cgtggcgctg ttctaatgca taaaggaaag aggattgaac catcatgggc ggacgtcaag aaagacctaa tatcatatgg aggaggctgg aagttagaag gagaatgga ggaggaga gagtccagg tattggcact gagcctgga aaaaatcca gagccgtcca aacgaaacct ggtcttttca aaaccaacgc cggaacaata ggtgctgtat ctctggactt ttctcctgga 4980. acgtcaggat ctccaattat cgacaaaaaa ggaaaagttg tgggtcttta tggtaatggt gttgttacaa ggagtggagc atatgtgagt gctatagccc agactgaaaa aagcattgaa gacaacccag agatcgaga tgacattttc cgaagaga gactgaccat catggacctc ccccaggag cgggaagac cttccggcca tagtcagaga agctaaaa cggggtttga cttggccccc actagagttg tggcagctga aatggagga gcccttagag gacttccaat aagataccag accccagcca tcagagctgt gcacaccggg 5280 cgggagatg tggacctaat gtgtcatgcc accattacca tgaggctgct atcaccagtt 5340 agagtgccaa actacaacct gattatcatg gacgaagccc atttcacaga cccagcaagt 5400 atagcagcta gaggatacat ctcactcga gtggagatgg gtgaggcagc tgggattttt 5460 atgacagcca ctcccccgggg aagcagagac ccattccctc agagcaatgc accaatcata 5520 gatgagaaa gagaatccc tgaacgctcg tggaattccg gatgaatg ggtcacggat 5580 tttaaaggga agactgttg gttcgttcca agtataaaag caggaatga tatagcagct 5640 tgcctgagga aaatggaaa gaagtgata caaccagta ggaagacctt tgattctgag 5700 tatgtcaaga ctagaaccaa tgattgggac ttcgtggtta caactgacat ttcagaatg 5760 ggtgccaatt tcaggctga gagggttata gacccagac gctgcatgaa accagtcata 5820 ctaacagatg gtgaagagcg gtgattctg gcaggaccta tgccagtgac ccacttagt 5880 gcagcacaaa gagagggaaaaggaaaatcaaaaaaaaaaagaaa ccagtacata 5940 6000 ctcctagata acatcaacac gccagaagga atcattccta gcatgttcga accagagcgt 6060 gaaaaggtgg atgccattga tggcgaatac cgcttgagag gagaagcaag gaaaaccttt 6120 gtagacttaa tgagaagagg agacctacca gtctggttgg cctacagagt ggcagctgaa 6180 ggcatcaact acgcagacag aaggtggtgt tttgatggag tcaagaacaa ccaaatccta 6240 gaaaaacg tggaagttga aatctggaca aaagaagggg aaaggaagaa attgaaaccc 6300 agatggttgg atgctaggat ctattctgac ccactggcgc taaaagaatt taggaattt 6360 gcagccggaa gaaagtctct gaccctgaac ctaatcacag aaatgggtag gctcccaacc 6420 ttcatgactc agaagcaag agacgcactg gacaacttag cagtgctgca cacggctgag 6480 gcaggtggaa gggcgtacaa ccatgctctc agtgaactgc cggagaccct ggagacattg 6540 cttttactga cacttctggc tacagtcacg ggagggatct ttttattctt gatgagcgca 6600 aggggcatag ggaagatgac cctgggaatg tgctgcataa tcacggctag catcctccta 6660 tggtacgcac aaatacagcc acactggata gcagctca taatactgga gttttctc 6720 atagttttgc ttattccaga acctgaaaaa cagagacac cccagacaa ccaactgacc 6780 tacgttgtca tagccatcct cacagtggtg gccgcaacca tggcaacga gatggttttc 6840 ctagaaaaaa cgaagaaga tctcggattg ggaagcattg caacccagca acccgagagc 6900 aacatcctgg acatagatct acgtcctgca tcagcatgga cgctgtatgc cgtggccaca 6960 acatttgtta caccaatgtt gagacatagc attgaaaatt cctcagtgaa tgtgtcccta 7020 acagctatag ccaaccaagc cacagtgtta atgggtctcg ggaaaggatg gccattgtca 7080 aagatggaca tcggagttcc ccttctcgcc attggatgct actcacaagt caacccata 7140 actctcatag cagctctttt cttattgta gcacattatg ccatcatagg gccaggactc 7200 caagcaaag caccagaa agctcagaaa agagcagcgg cgggcatcat gaaaaaccca 7260 actgtcgatg gaataacagt gattgaccta gatccaatac cttatgatcc gaagtttgaa 7320 aagcagttgg gatagtaat gctcctagtc ctctgcgtga ctcaagtatt gatgatgagg 7380 actacatggg ctctgtgtga ggctttaacc ttagctaccg ggcccactc cacattgtgg 7440 gaaggaaatc cagggaggtt ttggacact accattgcgg tgtcaatggc tacattttt 7500 agagggagtt acttggccgg agctggactt ctctttcta ttatgagaa cacaccac 7560 acaagaaggg gaactggcaa cataggagag acgcttggag agaatggaa aagccgattg 7620 aacgcattgg gaaaaagtga attccagatc tacaagaaa gtggaatcca ggaagtggat 7680 agaaccttag caaagaagg cattaaaaga ggagaaacgg accacacgc tgtgtcgcga 7740 ggctcagcaa aactgagatg gttcgttgag agaaacatgg tcacaccaga agggaagta 7800 gtggacctcg gttgtggcag aggaggctgg tcatactatt gtggaggact aagaatgta 7860 agagaagtca aaggcctaac aaaaggagga ccaggacacg aagaacccat ccccatgtca 7920 acatagggt ggaatctagt gcgtcttcaa agtggagttg acgttttctt catcccgcca 7980 gaaaagtgtg acacattatt gtgtgacata ggggagtcat caccaatcc cacagtggaa 8040 gcaggacgaa cactcagagt ccttaactta gtagaaaatt ggttgacaa cacactca 8100 ttttgcataa aggttctcaa cccatatatg ccctcagtca tagaaaaaat ggaagcacta 8160 caaaggaaat atggaggagc cttagtgagg aatccactct cacgaaactc cacacatgag 8220 atgtactggg tatccaatgc ttccgggaac atagtgtcat cagtgaacat gatttcaagg 8280 atgttgatca acagatttac aatgagatac aagaaagcca cttacgagcc ggatgttgac 8340 ctcggaagcg gaacccgtaa catcgggatt gaaagtgaga taccaaacct agatataatt 8400 gggaaaagaa tagaaaaaat aaagcaagag catgaaacat catggcacta tgaccaagac 8460 cacccataca aaacgtgggc ataccatggt agctatgaaa caaaacagac tggatcagca 8520 tcatccatgg tcaacggagt ggtcaggctg ctgacaaaac cttgggacgt cgtccccatg 8580 gtgacacaga tggcaatgac agacacgact ccatttggac aacagcgcgt ttttaaagag 8640 aaagtggaca cgagaaccca agaaccgaaa gaaggcacga agaaactaat gaaaataaca 8700 gcagagtggc tttggaaaga attagggaag aaaaagacac ccaggatgtg caccagagaa 8760 gaattcacaa gaaaggtgag aagcaatgca gccttggggg ccatattcac tgatgagaac 8820 aagtggaagt cggcacgtga ggctgttgaa gatagtaggt tttgggagct ggttgacaag 8880 gaaaggaatc tccatcttga aggaaagtgt gaaacatgtg tgtacaacat gatgggaaaa 8940 aggagaga agctagggga attcggcaag gcaaaggca gcagagccat atggtacatg 9000 tggcttggag cacgcttctt agagtttga gccctaggat tctttaatga agatcactgg 9060 ttctccagag agaactccct gagtggagtg gaaggagaag ggctgcacaa gctaggttac 9120 attctaagag acgtgagcaa gaagaggga ggagcaatgt atgccgatga caccgcagga 9180 tgggatacaa gatcacacact agaagaccta aaaaatgaag aaatggtaac aaaccacatg 9240 gaaggagaac acaagaact agccgaggcc atttcaac taacgtacca aaacaggtg 9300 gtgcgtgtgc aaagaccaac accaagaggc acagtaatgg acatcatatc gagagagac 9360 haaagaggta gtggacaagt tggcacctat ggactcaata ctttcaccaa tatggaagcc 9420 caactaatca gagatgga gggagaagga gtctttaaaa gcattcagca cctaacaatc 9480 acagaagaaa tcgctgtgca aactggtta gcaagagtgg ggcgcgaaag gttatcaaga 9540 atggccatca gtggagatga ttgtgttgtg aaacctttag atgacaggtt cgcaagcgct 9600 ttaacagctc taaatgacat gggaagatt aggaagaca tacacaatg ggaaccttca 9660 agaggatgga atgattggac acaagtgccc ttctgttcac accattcca tgagttaatc 9720 atgaaagacg gtcgcgtact cgttgttcca tgtagaaacc aagatgaact gattggcaga 9780 gcccgaatct cccaaggagc agggtggtct ttgcgggaga cggcctgtttt ggggaagtct 9840 tacgcccaaa tgtggagctt gatgtacttc cacagacgcg acctcaggct ggcggcaaat 9900 gctatttgct cggcagtacc atcacattgg gttccacaa gtcgacaac ctggtccata 9960 catgctaaac atgaatggat vakaacggaa gatatgctga cagtctggaa cagggtgtgg 10020 attcaagaaa acccatggat ggaagacaaa actccagtgg aatcatggga ggaatccca 10080 tacttgggga aaagagaga ccaatggtgc ggctcattga ttggttaac aagcaggggcc 10140 acctgggcaa agacaatcca agcagcaata aatcaagtta gatcccttat aggcaatgaa 10200 gatacacag attacatgcc atccatgaaa agatcagaa gagagagga agagagaga 10260 gttctgtggt agaaagcaaa actaacatga aacaaggcta gaagtcaggt cggattaagc catagtacgg aaaaaactat gctacctgtg agccccgtcc aaggacgtta aaagagtca 10380. ggccatcata aatgccatag cttgagtaaa cttgcagcc tgtagctcca cctgagaagg tgtaaaaaat ccggggaggcc acaaaccatg gaagctgtac gcatggcgta gtggactagc ggttagagga gacccctccc ttacaaatcg cagcaacaat ggggcccaa ggcgagatga agctgtagtc tcgctggaag gactagaggt tagaggagac ccccccgaaa caaaaaacag catattgacg ctgggaaaga ccagagatcc tgctgtctcc tcagcatcat tccaggcaca gacgccaga aaatggaatg gtgctgttga atcaacaggt tct <210> 6 <211> 3391 <212> PRT <213> The snowstorm <220> <223> High-Speed ​​Container Set 1,WVS <220> <221> misc_feature <222> (763)..(763) <223> Xaa snowflake snowflakes <400> 6 Met Asn Asn Gln Arg Lys Lys Ala Lys Asn Thr Pro Phe Asn Met Leu 1 5 10 15 Lys Arg Glu Arg Asn Arg Val Ser Thr Val Gln Gln Leu Thr Lys Arg 20 25 30 Phe Ser Leu Gly Met Leu Gln Gly Arg Gly Pro Leu Lys Leu Phe Met 35 40 45 Ala Leu Val Ala Phe Leu Arg Phe Leu Thr Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Lys Arg Trp Gly Thr Ile Lys Lys Ser Lys Ala Ile Asn Val 65 70 75 80 Leu Arg Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn 85 90 95 Arg Arg Arg Arg Ser Ala Gly Met Ile Ile Met Leu Ile Pro Thr Val 100 105 110 Met Ala Phe His Leu Thr Thr Arg Gly Gly Glu Pro His Met Ile Val 115 120 125 Ser Lys Gln Glu Arg Gly Lys Ser Leu Leu Phe Lys Thr Ser Ala Gly 130 135 140 Val Asn Met Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Met Thr Tyr Lys Cys Pro Arg Ile Thr Glu Ala Glu Pro Asp 165 170 175 Asp Val Asp Cys Trp Cys Asn Ala Thr Asp Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Ser Gln Thr Gly Glu His Arg Arg Asp Lys Arg Ser Val Ala 195 200 205 Leu Ala Pro His Val Gly Leu Gly Leu Glu Thr Arg Ala Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys Gln Ile Gln Lys Val Glu Thr Trp 225 230 235 240 Ala Leu Arg His Pro Gly Phe Thr Val Ile Ala Leu Phe Leu Ala His 245 250 255 Ala Ile Gly Thr Ser Ile Thr Gln Lys Gly Ile Ile Phe Ile Leu Leu 260 265 270 Met Leu Val Thr Pro Ser Met Ala Met Arg Cys Val Gly Ile Gly Asn 275 280 285 Arg Asp Phe Val Glu Gly Leu Ser Gly Ala Thr Trp Val Asp Val Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Ile Glu Leu Leu Lys Thr Glu Val Thr Asn Pro Ala Val Leu 325 330 335 Arg Lys Leu Cys Ile Glu Ala Lys Ile Ser Asn Thr Thr Thr Asp Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Ala Thr Leu Val Glu Glu Gln Asp Ala 355 360 365 Asn Phe Val Cys Arg Arg Thr Phe Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Ser Leu Ile Thr Cys Ala Lys Phe Lys 385 390 395 400 Cys Val Thr Lys Leu Glu Gly Lys Ile Val Gln Tyr Glu Asn Leu Lys 405 410 415 Tyr Ser Val Ile Val Thr Val His Thr Gly Asp Gln His Gln Val Gly 420 425 430 Asn Glu Thr Thr Glu His Gly Thr Thr Ala Thr Ile Thr Pro Gln Ala 435 440 445 Pro Thr Ser Glu Ile Gln Leu Thr Asp Tyr Gly Thr Leu Thr Leu Asp 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Thr 465 470 475 480 Met Lys Glu Arg Ser Trp Leu Val His Lys Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Thr Ser Gly Ala Ser Thr Ser Gln Glu Thr Trp Asn 500 505 510 Arg Gln Asp Leu Leu Val Thr Phe Lys Thr Ala His Ala Lys Lys Gln 515 520 525 Glu Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Thr Ser Gly Thr Thr Thr Ile Phe Ala 545 550 555 560 Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Thr Leu Lys Gly 565 570 575 Met Ser Tyr Val Met Cys Thr Gly Ser Phe Lys Leu Glu Lys Glu Val 580 585 590 Ala Glu Thr Gln His Gly Thr Val Leu Val Gln Val Lys Tyr Glu Gly 595 600 605 Thr Asp Ala Pro Cys Lys Ile Pro Phe Ser Thr Gln Asp Glu Lys Gly 610 615 620 Ala Thr Gln Asn Gly Arg Leu Ile Thr Ala Asn Pro Ile Val Thr Asp 625 630 635 640 Lys Glu Lys Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser 645 650 655 Tyr Ile Val Val Gly Ala Gly Glu Lys Ala Leu Lys Leu Ser Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Lys Met Phe Glu Ala Thr Ala Arg Gly 675 680 685 Ala Arg Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Ile Gly Gly Val Phe Thr Ser Met Gly Lys Leu Val His Gln Val Phe 705 710 715 720 Gly Thr Ala Tyr Gly Val Leu Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Gly Ile Gly Ile Leu Leu Thr Trp Leu Gly Leu Asn Ser Arg Asn 740 745 750 Thr Ser Leu Ser Met Met Cys Ile Ala Ala Xaa Ile Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Val Ser Trp Lys 770 775 780 Asn Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Ile Thr Asp Asn Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ser Lys 805 810 815 Leu Ala Ser Ala Ile Gln Lys Ala His Glu Glu Asp Ile Cys Gly Ile 820 825 830 Arg Ser Val Thr Arg Leu Glu Asn Leu Met Trp Lys Gln Ile Thr Pro 835 840 845 Glu Leu Asn His Ile Leu Ser Glu Asn Glu Val Lys Leu Thr Ile Met 850 855 860 Thr Gly Asp Ile Lys Gly Ile Met Gln Ala Gly Lys Arg Ser Leu Arg 865 870 875 880 Pro Gln Pro Thr Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala 885 890 895 Lys Met Leu Ser Thr Glu Ser His Asn Gln Thr Phe Leu Ile Asp Gly 900 905 910 Pro Glu Thr Ala Glu Cys Pro Asn Thr Asn Arg Ala Trp Asn Ser Leu 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Lys Glu Lys Gln Asp Val Phe Cys Asp Ser Lys Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Asn Arg Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Ala Leu Asn Asp Thr Trp Lys Ile Glu Lys Ala Ser Phe 980 985 990 Ile Glu Val Lys Asn Cys His Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Asn Leu Ala 1010 1015 1020 Gly Pro Val Ser Gln His Asn Tyr Arg Pro Gly Tyr His Thr Gln 1025 1030 1035 Ile Thr Gly Pro Trp His Leu Gly Lys Leu Glu Met Asp Phe Asp 1040 1045 1050 Phe Cys Asp Gly Thr Thr Val Val Val Thr Glu Asp Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Ile 1070 1075 1080 Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr 1085 1090 1095 Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala Gly 1115 1120 1125 His Gly Gln Val Asp Asn Phe Ser Leu Gly Val Leu Gly Met Ala 1130 1135 1140 Leu Phe Leu Glu Glu Met Leu Arg Thr Arg Val Gly Thr Lys His 1145 1150 1155 Ala Ile Leu Leu Val Ala Val Ser Phe Val Thr Leu Ile Thr Gly 1160 1165 1170 Asn Met Ser Phe Arg Asp Leu Gly Arg Val Met Val Met Val Gly 1175 1180 1185 Ala Thr Met Thr Asp Asp Ile Gly Met Gly Val Thr Tyr Leu Ala 1190 1195 1200 Leu Leu Ala Ala Phe Lys Val Arg Pro Thr Phe Ala Ala Gly Leu 1205 1210 1215 Leu Leu Arg Lys Leu Thr Ser Lys Glu Leu Met Met Thr Thr Ile 1220 1225 1230 Gly Ile Val Leu Leu Ser Gln Ser Thr Leu Pro Glu Thr Ile Leu 1235 1240 1245 Glu Leu Thr Asp Ala Leu Ala Leu Gly Met Met Val Leu Lys Met 1250 1255 1260 Val Arg Asn Met Glu Lys Tyr Gln Leu Ala Val Thr Ile Met Ala 1265 1270 1275 Ile Leu Cys Val Pro Asn Ala Val Ile Leu Gln Asn Ala Trp Lys 1280 1285 1290 Val Ser Cys Thr Ile Leu Ala Val Val Ser Val Ser Pro Leu Phe 1295 1300 1305 Leu Thr Ser Ser Gln Gln Lys Thr Asp Trp Ile Pro Leu Ala Leu 1310 1315 1320 Thr Ile Lys Gly Leu Asn Pro Thr Ala Ile Phe Leu Thr Thr Leu 1325 1330 1335 Ser Arg Thr Ser Lys Lys Arg Ser Trp Pro Leu Asn Glu Ala Ile 1340 1345 1350 Met Ala Val Gly Met Val Ser Ile Leu Ala Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Ile Pro Met Thr Gly Pro Leu Val Ala Gly Gly Leu Leu 1370 1375 1380 Thr Val Cys Tyr Val Leu Thr Gly Arg Ser Ala Asp Leu Glu Leu 1385 1390 1395 Glu Arg Ala Ala Asp Val Lys Trp Glu Asp Gln Ala Glu Ile Ser 1400 1405 1410 Gly Ser Ser Pro Ile Leu Ser Ile Thr Ile Ser Glu Asp Gly Ser 1415 1420 1425 Met Ser Ile Lys Asn Glu Glu Glu Asp Gln Thr Leu Thr Ile Leu 1430 1435 1440 Ile Arg Thr Gly Leu Leu Val Ile Ser Gly Leu Phe Pro Val Ser 1445 1450 1455 Ile Pro Ile Thr Ala Ala Ala Trp Tyr Leu Trp Glu Val Lys Lys 1460 1465 1470 Gln Arg Ala Gly Val Leu Trp Asp Val Pro Ser Pro Pro Pro Met 1475 1480 1485 Gly Lys Ala Glu Leu Glu Asp Gly Ala Tyr Arg Ile Lys Gln Lys 1490 1495 1500 Gly Ile Leu Gly Tyr Ser Gln Ile Gly Ala Gly Val Tyr Lys Glu 1505 1510 1515 Gly Thr Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met His Lys Gly Lys Arg Ile Glu Pro Ser Trp Ala Asp Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Lys Leu Glu Gly Glu 1550 1555 1560 Trp Lys Glu Gly Glu Glu Val Gln Val Leu Ala Leu Glu Pro Gly 1565 1570 1575 Lys Asn Pro Arg Ala Val Gln Thr Lys Pro Gly Leu Phe Lys Thr 1580 1585 1590 Asn Ala Gly Thr Ile Gly Ala Val Ser Leu Asp Phe Ser Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Ile Asp Lys Lys Gly Lys Val Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Arg Ser Gly Ala Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Thr Glu Lys Ser Ile Glu Asp Asn Pro Glu Ile 1640 1645 1650 Glu Asp Asp Ile Phe Arg Lys Arg Arg Leu Thr Ile Met Asp Leu 1655 1660 1665 His Pro Gly Ala Gly Lys Thr Lys Arg Tyr Leu Pro Ala Ile Val 1670 1675 1680 Arg Glu Ala Ile Lys Arg Gly Leu Arg Thr Leu Ile Leu Ala Pro 1685 1690 1695 Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu 1700 1705 1710 Pro Ile Arg Tyr Gln Thr Pro Ala Ile Arg Ala Val His Thr Gly 1715 1720 1725 Arg Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg 1730 1735 1740 Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met 1745 1750 1755 Asp Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly 1760 1765 1770 Tyr Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Gly Ile Phe 1775 1780 1785 Met Thr Ala Thr Pro Pro Gly Ser Arg Asp Pro Phe Pro Gln Ser 1790 1795 1800 Asn Ala Pro Ile Ile Asp Glu Glu Arg Glu Ile Pro Glu Arg Ser 1805 1810 1815 Trp Asn Ser Gly His Glu Trp Val Thr Asp Phe Lys Gly Lys Thr 1820 1825 1830 Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Ala 1835 1840 1845 Cys Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys 1850 1855 1860 Thr Phe Asp Ser Glu Tyr Val Lys Thr Arg Thr Asn Asp Trp Asp 1865 1870 1875 Phe Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys 1880 1885 1890 Ala Glu Arg Val Ile Asp Pro Arg Arg Cys Met Lys Pro Val Ile 1895 1900 1905 Leu Thr Asp Gly Glu Glu Arg Val Ile Leu Ala Gly Pro Met Pro 1910 1915 1920 Val Thr His Ser Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg 1925 1930 1935 Asn Pro Lys Asn Glu Asn Asp Gln Tyr Ile Tyr Met Gly Glu Pro 1940 1945 1950 Leu Glu Asn Asp Glu Asp Cys Ala His Trp Lys Glu Ala Lys Met 1955 1960 1965 Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ser Met 1970 1975 1980 Phe Glu Pro Glu Arg Glu Lys Val Asp Ala Ile Asp Gly Glu Tyr 1985 1990 1995 Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Asp Leu Met Arg 2000 2005 2010 Arg Gly Asp Leu Pro Val Trp Leu Ala Tyr Arg Val Ala Ala Glu 2015 2020 2025 Gly Ile Asn Tyr Ala Asp Arg Arg Trp Cys Phe Asp Gly Val Lys 2030 2035 2040 Asn Asn Gln Ile Leu Glu Glu Asn Val Glu Val Glu Ile Trp Thr 2045 2050 2055 Lys Glu Gly Glu Arg Lys Lys Leu Lys Pro Arg Trp Leu Asp Ala 2060 2065 2070 Arg Ile Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Glu Phe 2075 2080 2085 Ala Ala Gly Arg Lys Ser Leu Thr Leu Asn Leu Ile Thr Glu Met 2090 2095 2100 Gly Arg Leu Pro Thr Phe Met Thr Gln Lys Ala Arg Asp Ala Leu 2105 2110 2115 Asp Asn Leu Ala Val Leu His Thr Ala Glu Ala Gly Gly Arg Ala 2120 2125 2130 Tyr Asn His Ala Leu Ser Glu Leu Pro Glu Thr Leu Glu Thr Leu 2135 2140 2145 Leu Leu Leu Thr Leu Leu Ala Thr Val Thr Gly Gly Ile Phe Leu 2150 2155 2160 Phe Leu Met Ser Ala Arg Gly Ile Gly Lys Met Thr Leu Gly Met 2165 2170 2175 Cys Cys Ile Ile Thr Ala Ser Ile Leu Leu Trp Tyr Ala Gln Ile 2180 2185 2190 Gln Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu 2195 2200 2205 Ile Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln 2210 2215 2220 Asp Asn Gln Leu Thr Tyr Val Val Ile Ala Ile Leu Thr Val Val 2225 2230 2235 Ala Ala Thr Met Ala Asn Glu Met Gly Phe Leu Glu Lys Thr Lys 2240 2245 2250 Lys Asp Leu Gly Leu Gly Ser Ile Ala Thr Gln Gln Pro Glu Ser 2255 2260 2265 Asn Ile Leu Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu 2270 2275 2280 Tyr Ala Val Ala Thr Thr Phe Val Thr Pro Met Leu Arg His Ser 2285 2290 2295 Ile Glu Asn Ser Ser Val Asn Val Ser Leu Thr Ala Ile Ala Asn 2300 2305 2310 Gln Ala Thr Val Leu Met Gly Leu Gly Lys Gly Trp Pro Leu Ser 2315 2320 2325 Lys Met Asp Ile Gly Val Pro Leu Leu Ala Ile Gly Cys Tyr Ser 2330 2335 2340 Gln Val Asn Pro Ile Thr Leu Ile Ala Ala Leu Phe Leu Leu Val 2345 2350 2355 Ala His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr 2360 2365 2370 Arg Glu Ala Gln Lys Arg Ala Ala Ala Gly Ile Met Lys Asn Pro 2375 2380 2385 Thr Val Asp Gly Ile Thr Val Ile Asp Leu Asp Pro Ile Pro Tyr 2390 2395 2400 Asp Pro Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val 2405 2410 2415 Leu Cys Val Thr Gln Val Leu Met Met Arg Thr Thr Trp Ala Leu 2420 2425 2430 Cys Glu Ala Leu Thr Leu Ala Thr Gly Pro Ile Ser Thr Leu Trp 2435 2440 2445 Glu Gly Asn Pro Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser 2450 2455 2460 Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu 2465 2470 2475 Leu Phe Ser Ile Met Lys Asn Thr Thr Asn Thr Arg Arg Gly Thr 2480 2485 2490 Gly Asn Ile Gly Glu Thr Leu Gly Glu Lys Trp Lys Ser Arg Leu 2495 2500 2505 Asn Ala Leu Gly Lys Ser Glu Phe Gln Ile Tyr Lys Lys Ser Gly 2510 2515 2520 Ile Gln Glu Val Asp Arg Thr Leu Ala Lys Glu Gly Ile Lys Arg 2525 2530 2535 Gly Glu Thr Asp His His Ala Val Ser Arg Gly Ser Ala Lys Leu 2540 2545 2550 Arg Trp Phe Val Glu Arg Asn Met Val Thr Pro Glu Gly Lys Val 2555 2560 2565 Val Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Gly 2570 2575 2580 Gly Leu Lys Asn Val Arg Glu Val Lys Gly Leu Thr Lys Gly Gly 2585 2590 2595 Pro Gly His Glu Glu Pro Ile Pro Met Ser Thr Tyr Gly Trp Asn 2600 2605 2610 Leu Val Arg Leu Gln Ser Gly Val Asp Val Phe Phe Ile Pro Pro 2615 2620 2625 Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro 2630 2635 2640 Asn Pro Thr Val Glu Ala Gly Arg Thr Leu Arg Val Leu Asn Leu 2645 2650 2655 Val Glu Asn Trp Leu Asn Asn Asn Thr Gln Phe Cys Ile Lys Val 2660 2665 2670 Leu Asn Pro Tyr Met Pro Ser Val Ile Glu Lys Met Glu Ala Leu 2675 2680 2685 Gln Arg Lys Tyr Gly Gly Ala Leu Val Arg Asn Pro Leu Ser Arg 2690 2695 2700 Asn Ser Thr His Glu Met Tyr Trp Val Ser Asn Ala Ser Gly Asn 2705 2710 2715 Ile Val Ser Ser Val Asn Met Ile Ser Arg Met Leu Ile Asn Arg 2720 2725 2730 Phe Thr Met Arg Tyr Lys Lys Ala Thr Tyr Glu Pro Asp Val Asp 2735 2740 2745 Leu Gly Ser Gly Thr Arg Asn Ile Gly Ile Glu Ser Glu Ile Pro 2750 2755 2760 Asn Leu Asp Ile Ile Gly Lys Arg Ile Glu Lys Ile Lys Gln Glu 2765 2770 2775 His Glu Thr Ser Trp His Tyr Asp Gln Asp His Pro Tyr Lys Thr 2780 2785 2790 Trp Ala Tyr His Gly Ser Tyr Glu Thr Lys Gln Thr Gly Ser Ala 2795 2800 2805 Ser Ser Met Val Asn Gly Val Val Arg Leu Leu Thr Lys Pro Trp 2810 2815 2820 Asp Val Val Pro Met Val Thr Gln Met Ala Met Thr Asp Thr Thr 2825 2830 2835 Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg 2840 2845 2850 Thr Gln Glu Pro Lys Glu Gly Thr Lys Lys Leu Met Lys Ile Thr 2855 2860 2865 Ala Glu Trp Leu Trp Lys Glu Leu Gly Lys Lys Lys Thr Pro Arg 2870 2875 2880 Met Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn Ala 2885 2890 2895 Ala Leu Gly Ala Ile Phe Thr Asp Glu Asn Lys Trp Lys Ser Ala 2900 2905 2910 Arg Glu Ala Val Glu Asp Ser Arg Phe Trp Glu Leu Val Asp Lys 2915 2920 2925 Glu Arg Asn Leu His Leu Glu Gly Lys Cys Glu Thr Cys Val Tyr 2930 2935 2940 Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys 2945 2950 2955 Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg 2960 2965 2970 Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp 2975 2980 2985 Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly Leu 2990 2995 3000 His Lys Leu Gly Tyr Ile Leu Arg Asp Val Ser Lys Lys Glu Gly 3005 3010 3015 Gly Ala Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile 3020 3025 3030 Thr Leu Glu Asp Leu Lys Asn Glu Glu Met Val Thr Asn His Met 3035 3040 3045 Glu Gly Glu His Lys Lys Leu Ala Glu Ala Ile Phe Lys Leu Thr 3050 3055 3060 Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Thr Pro Arg Gly 3065 3070 3075 Thr Val Met Asp Ile Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly 3080 3085 3090 Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala 3095 3100 3105 Gln Leu Ile Arg Gln Met Glu Gly Glu Gly Val Phe Lys Ser Ile 3110 3115 3120 Gln His Leu Thr Ile Thr Glu Glu Ile Ala Val Gln Asn Trp Leu 3125 3130 3135 Ala Arg Val Gly Arg Glu Arg Leu Ser Arg Met Ala Ile Ser Gly 3140 3145 3150 Asp Asp Cys Val Val Lys Pro Leu Asp Asp Arg Phe Ala Ser Ala 3155 3160 3165 Leu Thr Ala Leu Asn Asp Met Gly Lys Ile Arg Lys Asp Ile Gln 3170 3175 3180 Gln Trp Glu Pro Ser Arg Gly Trp Asn Asp Trp Thr Gln Val Pro 3185 3190 3195 Phe Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg 3200 3205 3210 Val Leu Val Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg 3215 3220 3225 Ala Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala 3230 3235 3240 Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe 3245 3250 3255 His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser Ala 3260 3265 3270 Val Pro Ser His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile 3275 3280 3285 His Ala Lys His Glu Trp Met Thr Thr Glu Asp Met Leu Thr Val 3290 3295 3300 Trp Asn Arg Val Trp Ile Gln Glu Asn Pro Trp Met Glu Asp Lys 3305 3310 3315 Thr Pro Val Glu Ser Trp Glu Glu Ile Pro Tyr Leu Gly Lys Arg 3320 3325 3330 Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala 3335 3340 3345 Thr Trp Ala Lys Asn Ile Gln Ala Ala Ile Asn Gln Val Arg Ser 3350 3355 3360 Leu Ile Gly Asn Glu Glu Tyr Thr Asp Tyr Met Pro Ser Met Lys 3365 3370 3375 Arg Phe Arg Arg Glu Glu Glu Glu Ala Gly Val Leu Trp 3380 3385 3390 <210>7 <211>10723 <212>DNA <213>Artificial Sequence <220> <223>Dengue virus serotype 1, BVS <400>7 agttgttagt ctacgtggac cgacaaagac agattctttg agggagctaa gctcaatgta 60 gttctaacag ttttttaatt agagagcaga tctctgatga ataaccaacg gaaaaaggcg 120 aaaaacacgc ctttcaatat gctgaaacgc gagagaaacc gcgtgtcgac tgtgcaacag 180 ctgacaaaga gattctcact tggaatgctg cagggacgag gaccattaaa actgttcatg 240 gccctggtgg cgttccttcg tttcctaaca atcccaccaa cagcagggat attgaagaga 300 tggggaacaa ttaaaaaatc aaaagctatt aatgttttga gagggttcag gaaagagatt 360 ggaaggatgc tgaacatctt gaataggaga cgcagatctg caggcatgat cattatgctg 420 attccaacag tgatggcgtt ccatttaacc acgcgtgggg gagagccgca tatgatagtt 480 agcaagcagg aaagaggaaa gtcacttttg ttcaagacct ctgcaggtgt caacatgtgc 540 accctcattg cgatggattt gggagagttg tgtgaggaca cgatgaccta caaatgcccc 600 cggatcactg aggcggaacc agatgacgtt gactgttggt gcaatgccac ggacacatgg 660 gtgacctatg gaacgtgctc tcaaactggc gaacaccgac gagacaaacg ttccgtcgca 720 ttggccccac acgtggggct tggcctagaa acaagagccg aaacgtggat gtcctctgaa 780 ggtgcttgga aacagataca aaaagtagag acttgggctc tgagacatcc aggattcacg 840 gtgatagccc tttttctagc acatgccata ggaacatcca tcacccagaa agggatcatt 900 ttcattttgc tgatgctggt aacaccatct atggccatgc gatgcgtggg aataggcaac 960 agagacttcg tggaaggact gtcaggagca acatgggtgg atgtggtact ggagcatgga 1020 agttgcgtca ccaccatggc aaaaaacaaa ccaacactgg acattgaact cttgaagacg 1080 gaggtcacaa accctgcagt tctgcgtaaa ttgtgcattg aagctaaaat atcaaacacc 1140 accaccgatt cgagatgtcc aacacaagga gaagccacac tggtggaaga acaagacgcg 1200 aactttgtgt gccgacgaac gttcgtggac agaggctggg gcaatggctg tgggctattc 1260 ggaaaaggta gtctaataac gtgtgccaag tttaagtgtg tgacaaaact agaaggaaag 1320 atagttcaat atgaaaacct aaaatattca gtgatagtca ccgtccacac tggagatcag 1380 caccaggtgg gaaatgagac tacagaacat ggaacaactg caaccataac acctcaagct 1440 cctacgtcgg aaatacagct gaccgactac ggaaccctta cattagattg ttcacctagg 1500 acagggctag attttaacga gatggtgttg ctgacaatga aagaaagatc atggcttgtc 1560 cacaaacaat ggttcctaga cttaccactg ccttggacct ctggggcttc aacatcccaa 1620 gagacttgga acagacaaga tttactggtc acatttaaga cagctcatgc aaagaagcag 1680 gaagtagtcg tactaggatc acaagaagga gcaatgcaca ctgcgctgac tggagcgaca 1740 gaaatccaaa cgtcaggaac gacaacaatt ttcgcaggac acctaaaatg cagactaaaa 1800 atggacaaac taactttaaa agggatgtca tatgtgatgt gcacaggctc attcaagtta 1860 gagaaagaag tggctgagac ccagcatgga actgttctgg tgcaggttaa atatgaagga 1920 acagacgcac catgcagat tcccttttcg acccaagatg agaaaggagc aacccagat gggagata taacagccaa ccccatagtc actgacaaag aaaaaccagt caatattgag gcagaaccac cctttggtga gagctacatc gtggtaggag caggtgaaaa agctttgaaa ctaagctggt tcaagaaagg aagcagcata gggaaaatgt ttgaagcaac tgcccgagga gcacgaagga tggccattct gggagacacc gcatgggact tcggttctat aggaggagtg ttcacgtcta tgggaaaact ggtacaccag gtttttgga ctgcatatgg agttttgttt agcggagttt cttggaccat gaaaatagga atagggattc tgctgacatg gctaggatta 2340 aattcaagga acacgtccct ttcgatgatg tgcatcgcag ccgccattgt gacactgtat ttgggagtca tggtgcaggc cgatagtggt tgcgttgtga gctggaaaaa caaagaactg aaatgtggca gtgggatttt catcacagac aacgtgcaca catggacaga acaatacaag ttccaaccag aatccccttc aaaactagct tcagctatcc agaaagccca tgaagaggac atttgtggaa tccgctcagt aacaagactg gagaatctga tgtggaaaca aatacacca gattgaatc acattctatc agaaaatgag gtgaagttaa ctattatgac aggagacatc 2700 aaaggaatca tgcaggcagg aaacgatct ctgcggccctc agcccactga gctgaagtat 2760 tcatggaaaa catggggcaa agcaaaatg ctctctacag agtctcataa ccagaccttt 2820 ctcattgatg gccccgaac agcagaatgc cccacaca atagagcttg gattcgttg 2880 gaagttgaag actatggctt tggagtattc accaccaata tattggctaaa attgaaagaa 2940 aaaacaggatg tattctgcga ctcaaactc atgtcagcgg ccaaaaga caacagagcc 3000 gtccatgccg attgggtta ttggatagaa agtgcactca atgacacatg gaagatagag 3060 aaagcctctt tcattgaagt taaaaactgc cactggccaa atcacacac cctctggagc 3120 aatggagtgc tagaagtga gatgatatt ccaagaatc tcgctggacc agtgtctca 3180 cacaactata gaccaggcta ccatacacaa atacaggac catggcatct aggtaagctt 3240 gagatgact tgatttctg tgatgacaca acagtgtag tgactgagga ctgcggaat 3300 agaggaccct ctttgagac aaccactgcc tctggaaac tcatacaga atggtgctgc 3360 cgatcttgca cattaccacc gctaagatac agaggtgagg atgggtgctg gtacgggatg 3420 gaatcagac cattgaagga gaagaagag aatttggtca actccttggt cacagctgga 3480 3540 atgcttagga cccgagtagg aacgaaacat gcaatactac tagttgcagt ttcttttgtg 3600 acattgatca cagggaacat gtcctttaga gacctgggaa gagtgatggt tatggtaggc 3660 gccactatga cggatgacat aggtatgggc gtgacttatc ttgccctact agcagccttc 3720 aaagtcagac caacttttgc agctggacta ctcttgagaa agctgacctc caaggaattg 3780 atgatgacta ctataggaat tgtactcctc tcccagagca ccctaccaga gaccattctt 3840 gagttgactg atgcgttagc cttaggcatg atggtcctca aaatggtgag aaatatggaa 3900 aagtatcaat tggcagtgac tatcatggct atcttgtgcg tcccaaacgc agtgatatta 3960 caaaacgcat ggaagtgag ttgcacaata ttggcagtgg tgtccgtttc cccactgttc 4020 ttaacatcct cacagcaaaa aacagattgg ataccattag cattgacgat caaaggtctc 4080 aatccaacag ctattttct aacaacccctc tcaagaacca gcaagaaaag gagctggcca ttaatgagg ctatcatggc agtcgggatg gtgagcattt tagccagttc tctcctaaaa aatgatattc ccatgacagg accattagtg gctggagggc tcctcactgt gtgctacgtg 4260 ctcactggac gatcggccga tttggaactg gagagagcag ccgatgtcaa atgggaagac 4380. 4380. 4380. 4380. 4380. 4380. 4380. 4380. 4380 atgtcgataa aaaatgaaga ggaagatcaa acactgacca tactcattag aacaggattg ctggtgatct caggactttt tcctgtatca attack cggcagcagc atggtacctg tgggaagtga agaaacaacg ggccggagta ttgtgggatg ttccttcacc cccacccatg 4560. ggaaaggctg aactggaaga tggagcctat agaattaagc aaaaagggat tcttggatat tcccagatcg gagccggagt ttacaaagaa ggaacattcc atacaatgtg gcatgtcaca cgtggcgctg ttctaatgca taaaggaaag aggattgaac catcatgggc ggacgtcaag aaagacctaa tatcatatgg aggaggctgg aagttagaag gagaatgga ggaggaga gaagtccagg tattggcact ggagcctgga aaaaatccaa gagccgtcca aacgaaacct 4860 ggtcttttca aaaccaacgc cggaacaata ggtgctgtat ctctggactt ttctcctgga 4920 acgtcaggat ctccaattat cgacaaaaaa ggaaaagttg tgggtcttta tggtaatggt 4980 gttgttacaa ggagtggagc atatgtgagt gctatagccc agactgaaaa aagcattgaa 5040 gacaacccag agatcgaaga tgacattttc cgaaagagaa gactgaccat catggacctc 5100 cacccaggag cgggaaagac gaagagatac cttccggcca tagtcagaga agctataaaaa 5160 cggggtttga gaacattaat cttggccccc actagagttg tggcagctga aatggaggaa 5220 gcccttagag gacttccaat aagataccag accccagcca tcagagctgt gcacaccggg 5280 cgggagattg tggacctaat gtgtcatgcc acatttacca tgaggctgct atcaccagtt 5340 agagtgccaa actacaacct gattatcatg gacgaagccc atttcacaga cccagcaagt 5400 atagcagcta gaggatacat ctcaactcga gtggagatgg gtgaggcagc tgggattttt 5460 atgacagcca ctccccccggg aagcagagac ccatttcctc agagcaatgc accaatcata 5520 gatgaagaaa gagaaatccc tgaacgctcg tggaattccg gacatgaatg ggtcacggat tttaaaggga agactgtttg gttcgttcca agtataaaag caggaaatga tatagcagct tgcctgagga aaaatggaaa gaaagtgata caactcagta ggaagacctt tgattctgag tatgtcaaga ctagaaccaa tgattgggac ttcgtggtta caactgacat ttcagaaatg ggtgccaatt tcaaggctga gagggttata gaccccagac gctgcatgaa accagtcata ctaacagatg gtgaagagcg ggtgattctg gcaggaccta tgccagtgac ccactctagt gcagcacaaa gaagagggag aataggaaga aatccaaaaa atgagaatga ccagtacata tacatggggg aacctctgga aaatgatgaa gactgtgcac actggaaaga agctaaaatg ctcctagata acatcaacac gccagaagga atcattccta gcatgttcga accagagcgt gaaaggtgg atgccattga tggcgaatac cgcttgagag gagaagcaag gaaaaaccttt gtagacttaa tgagaagagg agacctacca gtctggttgg cctacagagt ggcagctgaa ggcatcaact acgcagacag aaggtggtgt tttgatggag tcaagaacaa ccaaatccta gaaaaacg tggaagttga aatctggaca aaagaagggg aaaggaagaa attgaaaccc 6300 agatggttgg atgctaggat ctattctgac ccactggcgc taaaagaatt taggaattt 6360 gcagccggaa gaaagtctct gaccctgaac ctaatcacag aaatgggtag gctcccaacc 6420 ttcatgactc agaagcaag agacgcactg gacaacttag cagtgctgca cacggctgag 6480 gcaggtggaa gggcgtacaa ccatgctctc agtgaactgc cggagaccct ggagacattg 6540 cttttactga cacttctggc tacagtcacg ggagggatct ttttattctt gatgagcgca 6600 aggggcatag ggaagatgac cctgggaatg tgctgcataa tcacggctag catcctccta 6660 tggtacgcac aaatacagcc acactggata gcagcttcaa taatactgga gttttttctc 6720 atagttttgc ttattccaga acctgaaaaa cagagaacac cccaagacaa ccaactgacc 6780 tacgttgtca tagccatcct cacagtggtg gccgcaacca tggcaaacga gatgggtttc 6840 ctagaaaaaa cgaaaaaga tctcggattg ggaagcattg caacccagca acccgagagc 6900 aacatcctgg acatagatct acgtcctgca tcagcatgga cgctgtatgc cgtggccaca 6960 acatttgtta caccaatgtt gagacatagc attgaaaatt cctcagtgaa tgtgtcccta 7020 acagctatag ccaaccaagc cacagtgtta atgggtctcg ggaaaggatg gccattgtca 7080 aagatggaca tcggagttcc ccttctcgcc attggatgct actcacaagt caacccata 7140 actctcatag cagctctttt cttattgta gcacattatg ccatcatagg gccaggactc 7200 caagcaaag caccagaa agctcagaaa agagcagcgg cgggcatcat gaaaaaccca 7260 actgtcgatg gaatacagt gattgaccta gatccaatac cttatgatcc gaagtttgaa 7320 aagcagttgg gatagtaat gctcctagtc ctctgcgtga ctcaagtatt gatgatgagg 7380 actacatggg ctctgtgtga ggctttaacc ttagctaccg ggcccactc cacattgtgg 7440 gaaggaaatc cagggaggtt ttggacact accattgcgg tgtcaatggc tacattttt 7500 agagggagtt acttggccgg agctggactt ctctttcta ttatgagaa cacaccac 7560 acaagaaggg gaactggcaa cataggagag acgcttggag agaatggaa aagccgattg 7620 aacgcattgg gaaaaagtga attccagatc tacaagaaa gtggaatcca ggaagtggat 7680 agaaccttag caaagaagg cattaaaaga ggagaaacgg accacacgc tgtgtcgcga 7740 ggctcagcaa aactgagatg gttcgttgag agaaacatgg tcacaccaga agggaagta 7800 gtggacctcg gttgtggcag aggaggctgg tcatactatt gtggaggact aagaatgta 7860 agagaagtca aaggcctaac aaaaggagga ccaggacacg aagaacccat ccccatgtca 7920 acatagggt ggaatctagt gcgtcttcaa agtggagttg acgttttctt catcccgcca 7980 gaaaagtgtg acacattatt gtgtgacata ggggagtcat caccaatcc cacagtggaa 8040 gcaggacgaa cactcagagt ccttaactta gtagaaaatt ggttgacaa cacactca 8100 ttttgcataa aggttctcaa cccatatatg ccctcagtca tagaaaaat ggaagcacta 8160 haaggaaat atggagc cttagtgagg aatccactct cacgaactc cacacatgag 8220 atgtactggg tatccaatgc ttccgggaac atagtgtcat cagtgaacat gatttcagg 8280 atgttgatca acgatttac aatgagatac aagaaagcca cttacgagcc ggatgttgac 8340 ctcggaagcg gaacccgtaa catcgggatt gaagtgaga taccaacct agatatatt 8400 gggaaaagaa tagaaaaaat aaagcaagag catgaaacat catggcacta tgaccaagac cacccataca aaacgtgggc ataccatggt agctatgaaa caaaacagac tggatcagca 8580. tcatccatgg tcaacggagt ggtcaggctg ctgacaaaac cttgggacgt cgtccccatg gtgacacaga tggcaatgac agacacgact ccatttggac aacagcgcgt ttttaaagag aaagtggaca cgagaaccca agaccgaa aagacga agaaactat gaaataca gcagagtggc tttggaaga attagggaag aaaaagacac ccaggatgtg caccagaga 8820. 8820. 8820. 8820. 8820. 8820. 8820. 8820. aagtggaagt cggcacgtga ggctgttgaa gatagtaggt tttgggagct ggttgacaag gaaaggaatc tccatcttga aggaaagtgt gaaacatgtg tgtacaacat gatgggaaaa aggagaaga agctagggga attcggcaag gcaaaaggca gcagagccat atggtacatg tggcttggag cacgcttctt agagtttga gccctaggat tcttaaatga agatcactgg 9060 ttctccagag agaactccct gagtggagtg gaggagag ggctgcacaa gctaggttac attctaagag acgtgagcaa gaagaggga ggagcaatgt atgccgatga caccgcagga 9180 tgggatacaa gatcacacact agaagaccta aaaaatgaag aaatggtaac aaaccacatg 9240 gaaggagaac acaagaact agccgaggcc atttcaac taacgtacca aaacaggtg 9300 gtgcgtgtgc aaagaccaac accaagaggc acagtaatgg acatcatatc gagagagac 9360 haaagaggta gtggacaagt tggcacctat ggactcaata ctttcaccaa tatggaagcc 9420 caactaatca gagatgga gggagaagga gtctttaaaa gcattcagca cctaacaatc 9480 acagaagaaa tcgctgtgca aactggtta gcaagagtgg ggcgcgaaag gttatcaaga 9540 atggccatca gtggagatga ttgtgttgtg aaacctttag atgacaggtt cgcaagcgct 9600 ttaacagctc taaatgacat gggaagatt aggaagaca tacacaatg ggaaccttca 9660 agaggatgga atgattggac acaagtgccc ttctgttcac accattcca tgagttaatc 9720 atgaaagacg gtcgcgtact cgttgttcca tgtagaaacc aagatgaact gattggcaga 9780 gcccgaatct cccaaggagc agggtggtct ttgcgggaga cggcctgtttt ggggaagtct 9840 tacgcccaaa tgtggagctt gatgtacttc cacagacgcg acctcaggct ggcggcaaat 9900 gctatttgct cggcagtacc atcacattgg gttccaacaa gtcgaacaac ctggtccata 9960 catgctaaac atgaatggat gacaacggaa gacatgctga cagtctggaa cagggtgtgg 10020 attcaagaaa acccatggat ggaagacaaa actccagtgg aatcatggga ggaaatccca 10080 tacttgggga aaagagaaga ccaatggtgc ggctcattga ttgggttaac aagcagggcc 10140 acctgggcaa agaacatcca agcagcaata aatcaagtta gatcccttat aggcaatgaa 10200 gaatacacag attacatgcc atccatgaaa agattcagaa gagaagagga agaagcagga 10260 gttctgtggt agaaagcaaa actaacatga aacaaggcta gaagtcaggt cggattaagc 10320 catagtacgg aaaaaactat gctacctgtg agccccgtcc aaggacgtta aaagaagtca 10380 ggccatcata aatgccatag cttgagtaaa ctatgcagcc tgtagctcca cctgagaagg 10440 tgtaaaaaat ccgggaggcc acaaaccatg gaagctgtac gcatggcgta gtggactagc 10500 ggttagagga gacccctccc ttacaaatcg cagcaacaat gggggcccaa ggcgagatga 10560 agctgtagtc tcgctgggaag gactagaggt tagaggac ccccccgaaa caaaaaacag 10620 catattgacg ctgggaaaga ccagagatcc tgctgtctcc tcagcatcat tccaggcaca 10680 gaacgccaga aaatggaatg gtgctgttga atcaacaggt tct 10723 <210> 8 <211> 3391 <212> PRT <213> artificial sequence <220> <223> Dengue fever virus serotype 1, BVS <400> 8 Met Asn Asn Gln Arg Lys Lys Ala Lys Asn Thr Pro Phe Asn Met Leu 1 5 10 15 Lys Arg Glu Arg Asn Arg Val Ser Thr Val Gln Gln Leu Thr Lys Arg 20 25 30 Phe Ser Leu Gly Met Leu Gln Gly Arg Gly Pro Leu Lys Leu Phe Met 35 40 45 Ala Leu Val Ala Phe Leu Arg Phe Leu Thr Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Lys Arg Trp Gly Thr Ile Lys Lys Ser Lys Ala Ile Asn Val 65 70 75 80 Leu Arg Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn 85 90 95 Arg Arg Arg Arg Ser Ala Gly Met Ile Ile Met Leu Ile Pro Thr Val 100 105 110 Met Ala Phe His Leu Thr Thr Arg Gly Gly Glu Pro His Met Ile Val 115 120 125 Ser Lys Gln Glu Arg Gly Lys Ser Leu Leu Phe Lys Thr Ser Ala Gly 130 135 140 Val Asn Met Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Met Thr Tyr Lys Cys Pro Arg Ile Thr Glu Ala Glu Pro Asp 165 170 175 Asp Val Asp Cys Trp Cys Asn Ala Thr Asp Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Ser Gln Thr Gly Glu His Arg Arg Asp Lys Arg Ser Val Ala 195 200 205 Leu Ala Pro His Val Gly Leu Gly Leu Glu Thr Arg Ala Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys Gln Ile Gln Lys Val Glu Thr Trp 225 230 235 240 Ala Leu Arg His Pro Gly Phe Thr Val Ile Ala Leu Phe Leu Ala His 245 250 255 Ala Ile Gly Thr Ser Ile Thr Gln Lys Gly Ile Ile Phe Ile Leu Leu 260 265 270 Met Leu Val Thr Pro Ser Met Ala Met Arg Cys Val Gly Ile Gly Asn 275 280 285 Arg Asp Phe Val Glu Gly Leu Ser Gly Ala Thr Trp Val Asp Val Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Ile Glu Leu Leu Lys Thr Glu Val Thr Asn Pro Ala Val Leu 325 330 335 Arg Lys Leu Cys Ile Glu Ala Lys Ile Ser Asn Thr Thr Thr Asp Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Ala Thr Leu Val Glu Glu Gln Asp Ala 355 360 365 Asn Phe Val Cys Arg Arg Thr Phe Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Ser Leu Ile Thr Cys Ala Lys Phe Lys 385 390 395 400 Cys Val Thr Lys Leu Glu Gly Lys Ile Val Gln Tyr Glu Asn Leu Lys 405 410 415 Tyr Ser Val Ile Val Thr Val His Thr Gly Asp Gln His Gln Val Gly 420 425 430 Asn Glu Thr Thr Glu His Gly Thr Thr Ala Thr Ile Thr Pro Gln Ala 435 440 445 Pro Thr Ser Glu Ile Gln Leu Thr Asp Tyr Gly Thr Leu Thr Leu Asp 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Thr 465 470 475 480 Met Lys Glu Arg Ser Trp Leu Val His Lys Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Thr Ser Gly Ala Ser Thr Ser Gln Glu Thr Trp Asn 500 505 510 Arg Gln Asp Leu Leu Val Thr Phe Lys Thr Ala His Ala Lys Lys Gln 515 520 525 Glu Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Thr Ser Gly Thr Thr Thr Ile Phe Ala 545 550 555 560 Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Thr Leu Lys Gly 565 570 575 Met Ser Tyr Val Met Cys Thr Gly Ser Phe Lys Leu Glu Lys Glu Val 580 585 590 Ala Glu Thr Gln His Gly Thr Val Leu Val Gln Val Lys Tyr Glu Gly 595 600 605 Thr Asp Ala Pro Cys Lys Ile Pro Phe Ser Thr Gln Asp Glu Lys Gly 610 615 620 Ala Thr Gln Asn Gly Arg Leu Ile Thr Ala Asn Pro Ile Val Thr Asp 625 630 635 640 Lys Glu Lys Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser 645 650 655 Tyr Ile Val Val Gly Ala Gly Glu Lys Ala Leu Lys Leu Ser Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Lys Met Phe Glu Ala Thr Ala Arg Gly 675 680 685 Ala Arg Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Ile Gly Gly Val Phe Thr Ser Met Gly Lys Leu Val His Gln Val Phe 705 710 715 720 Gly Thr Ala Tyr Gly Val Leu Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Gly Ile Gly Ile Leu Leu Thr Trp Leu Gly Leu Asn Ser Arg Asn 740 745 750 Thr Ser Leu Ser Met Met Cys Ile Ala Ala Ala Ile Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Val Ser Trp Lys 770 775 780 Asn Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Ile Thr Asp Asn Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ser Lys 805 810 815 Leu Ala Ser Ala Ile Gln Lys Ala His Glu Glu Asp Ile Cys Gly Ile 820 825 830 Arg Ser Val Thr Arg Leu Glu Asn Leu Met Trp Lys Gln Ile Thr Pro 835 840 845 Glu Leu Asn His Ile Leu Ser Glu Asn Glu Val Lys Leu Thr Ile Met 850 855 860 Thr Gly Asp Ile Lys Gly Ile Met Gln Ala Gly Lys Arg Ser Leu Arg 865 870 875 880 Pro Gln Pro Thr Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala 885 890 895 Lys Met Leu Ser Thr Glu Ser His Asn Gln Thr Phe Leu Ile Asp Gly 900 905 910 Pro Glu Thr Ala Glu Cys Pro Asn Thr Asn Arg Ala Trp Asn Ser Leu 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Lys Glu Lys Gln Asp Val Phe Cys Asp Ser Lys Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Asn Arg Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Ala Leu Asn Asp Thr Trp Lys Ile Glu Lys Ala Ser Phe 980 985 990 Ile Glu Val Lys Asn Cys His Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Asn Leu Ala 1010 1015 1020 Gly Pro Val Ser Gln His Asn Tyr Arg Pro Gly Tyr His Thr Gln 1025 1030 1035 Ile Thr Gly Pro Trp His Leu Gly Lys Leu Glu Met Asp Phe Asp 1040 1045 1050 Phe Cys Asp Gly Thr Thr Val Val Val Thr Glu Asp Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Ile 1070 1075 1080 Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr 1085 1090 1095 Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala Gly 1115 1120 1125 His Gly Gln Val Asp Asn Phe Ser Leu Gly Val Leu Gly Met Ala 1130 1135 1140 Leu Phe Leu Glu Glu Met Leu Arg Thr Arg Val Gly Thr Lys His 1145 1150 1155 Ala Ile Leu Leu Val Ala Val Ser Phe Val Thr Leu Ile Thr Gly 1160 1165 1170 Asn Met Ser Phe Arg Asp Leu Gly Arg Val Met Val Met Val Gly 1175 1180 1185 Ala Thr Met Thr Asp Asp Ile Gly Met Gly Val Thr Tyr Leu Ala 1190 1195 1200 Leu Leu Ala Ala Phe Lys Val Arg Pro Thr Phe Ala Ala Gly Leu 1205 1210 1215 Leu Leu Arg Lys Leu Thr Ser Lys Glu Leu Met Met Thr Thr Ile 1220 1225 1230 Gly Ile Val Leu Leu Ser Gln Ser Thr Leu Pro Glu Thr Ile Leu 1235 1240 1245 Glu Leu Thr Asp Ala Leu Ala Leu Gly Met Met Val Leu Lys Met 1250 1255 1260 Val Arg Asn Met Glu Lys Tyr Gln Leu Ala Val Thr Ile Met Ala 1265 1270 1275 Ile Leu Cys Val Pro Asn Ala Val Ile Leu Gln Asn Ala Trp Lys 1280 1285 1290 Val Ser Cys Thr Ile Leu Ala Val Val Ser Val Ser Pro Leu Phe 1295 1300 1305 Leu Thr Ser Ser Gln Gln Lys Thr Asp Trp Ile Pro Leu Ala Leu 1310 1315 1320 Thr Ile Lys Gly Leu Asn Pro Thr Ala Ile Phe Leu Thr Thr Leu 1325 1330 1335 Ser Arg Thr Ser Lys Lys Arg Ser Trp Pro Leu Asn Glu Ala Ile 1340 1345 1350 Met Ala Val Gly Met Val Ser Ile Leu Ala Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Ile Pro Met Thr Gly Pro Leu Val Ala Gly Gly Leu Leu 1370 1375 1380 Thr Val Cys Tyr Val Leu Thr Gly Arg Ser Ala Asp Leu Glu Leu 1385 1390 1395 Glu Arg Ala Ala Asp Val Lys Trp Glu Asp Gln Ala Glu Ile Ser 1400 1405 1410 Gly Ser Ser Pro Ile Leu Ser Ile Thr Ile Ser Glu Asp Gly Ser 1415 1420 1425 Met Ser Ile Lys Asn Glu Glu Glu Asp Gln Thr Leu Thr Ile Leu 1430 1435 1440 Ile Arg Thr Gly Leu Leu Val Ile Ser Gly Leu Phe Pro Val Ser 1445 1450 1455 Ile Pro Ile Thr Ala Ala Ala Trp Tyr Leu Trp Glu Val Lys Lys 1460 1465 1470 Gln Arg Ala Gly Val Leu Trp Asp Val Pro Ser Pro Pro Pro Met 1475 1480 1485 Gly Lys Ala Glu Leu Glu Asp Gly Ala Tyr Arg Ile Lys Gln Lys 1490 1495 1500 Gly Ile Leu Gly Tyr Ser Gln Ile Gly Ala Gly Val Tyr Lys Glu 1505 1510 1515 Gly Thr Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met His Lys Gly Lys Arg Ile Glu Pro Ser Trp Ala Asp Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Lys Leu Glu Gly Glu 1550 1555 1560 Trp Lys Glu Gly Glu Glu Val Gln Val Leu Ala Leu Glu Pro Gly 1565 1570 1575 Lys Asn Pro Arg Ala Val Gln Thr Lys Pro Gly Leu Phe Lys Thr 1580 1585 1590 Asn Ala Gly Thr Ile Gly Ala Val Ser Leu Asp Phe Ser Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Ile Asp Lys Lys Gly Lys Val Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Arg Ser Gly Ala Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Thr Glu Lys Ser Ile Glu Asp Asn Pro Glu Ile 1640 1645 1650 Glu Asp Asp Ile Phe Arg Lys Arg Arg Leu Thr Ile Met Asp Leu 1655 1660 1665 His Pro Gly Ala Gly Lys Thr Lys Arg Tyr Leu Pro Ala Ile Val 1670 1675 1680 Arg Glu Ala Ile Lys Arg Gly Leu Arg Thr Leu Ile Leu Ala Pro 1685 1690 1695 Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu 1700 1705 1710 Pro Ile Arg Tyr Gln Thr Pro Ala Ile Arg Ala Val His Thr Gly 1715 1720 1725 Arg Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg 1730 1735 1740 Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met 1745 1750 1755 Asp Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly 1760 1765 1770 Tyr Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Gly Ile Phe 1775 1780 1785 Met Thr Ala Thr Pro Pro Gly Ser Arg Asp Pro Phe Pro Gln Ser 1790 1795 1800 Asn Ala Pro Ile Ile Asp Glu Glu Arg Glu Ile Pro Glu Arg Ser 1805 1810 1815 Trp Asn Ser Gly His Glu Trp Val Thr Asp Phe Lys Gly Lys Thr 1820 1825 1830 Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Ala 1835 1840 1845 Cys Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys 1850 1855 1860 Thr Phe Asp Ser Glu Tyr Val Lys Thr Arg Thr Asn Asp Trp Asp 1865 1870 1875 Phe Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys 1880 1885 1890 Ala Glu Arg Val Ile Asp Pro Arg Arg Cys Met Lys Pro Val Ile 1895 1900 1905 Leu Thr Asp Gly Glu Glu Arg Val Ile Leu Ala Gly Pro Met Pro 1910 1915 1920 Val Thr His Ser Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg 1925 1930 1935 Asn Pro Lys Asn Glu Asn Asp Gln Tyr Ile Tyr Met Gly Glu Pro 1940 1945 1950 Leu Glu Asn Asp Glu Asp Cys Ala His Trp Lys Glu Ala Lys Met 1955 1960 1965 Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ser Met 1970 1975 1980 Phe Glu Pro Glu Arg Glu Lys Val Asp Ala Ile Asp Gly Glu Tyr 1985 1990 1995 Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Asp Leu Met Arg 2000 2005 2010 Arg Gly Asp Leu Pro Val Trp Leu Ala Tyr Arg Val Ala Ala Glu 2015 2020 2025 Gly Ile Asn Tyr Ala Asp Arg Arg Trp Cys Phe Asp Gly Val Lys 2030 2035 2040 Asn Asn Gln Ile Leu Glu Glu Asn Val Glu Val Glu Ile Trp Thr 2045 2050 2055 Lys Glu Gly Glu Arg Lys Lys Leu Lys Pro Arg Trp Leu Asp Ala 2060 2065 2070 Arg Ile Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Glu Phe 2075 2080 2085 Ala Ala Gly Arg Lys Ser Leu Thr Leu Asn Leu Ile Thr Glu Met 2090 2095 2100 Gly Arg Leu Pro Thr Phe Met Thr Gln Lys Ala Arg Asp Ala Leu 2105 2110 2115 Asp Asn Leu Ala Val Leu His Thr Ala Glu Ala Gly Gly Arg Ala 2120 2125 2130 Tyr Asn His Ala Leu Ser Glu Leu Pro Glu Thr Leu Glu Thr Leu 2135 2140 2145 Leu Leu Leu Thr Leu Leu Ala Thr Val Thr Gly Gly Ile Phe Leu 2150 2155 2160 Phe Leu Met Ser Ala Arg Gly Ile Gly Lys Met Thr Leu Gly Met 2165 2170 2175 Cys Cys Ile Ile Thr Ala Ser Ile Leu Leu Trp Tyr Ala Gln Ile 2180 2185 2190 Gln Pro His Trp Ile Ala Ala Ser Ile lle Leu Glu Phe Phe Leu 2195 2200 2205 Ile Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln 2210 2215 2220 Asp Asn Gln Leu Thr Tyr Val Val Ile Ala Ile Leu Thr Val Val 2225 2230 2235 Ala Ala Thr Met Ala Asn Glu Met Gly Phe Leu Glu Lys Thr Lys 2240 2245 2250 Lys Asp Leu Gly Leu Gly Ser Ile Ala Thr Gln Gln Pro Glu Ser 2255 2260 2265 Asn Ile Leu Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu 2270 2275 2280 Tyr Ala Val Ala Thr Thr Phe Val Thr Pro Met Leu Arg His Ser 2285 2290 2295 Ile Glu Asn Ser Ser Val Asn Val Ser Leu Thr Ala Ile Ala Asn 2300 2305 2310 Gln Ala Thr Val Leu Met Gly Leu Gly Lys Gly Trp Pro Leu Ser 2315 2320 2325 Lys Met Asp Ile Gly Val Pro Leu Leu Ala Ile Gly Cys Tyr Ser 2330 2335 2340 Gln Val Asn Pro Ile Thr Leu Ile Ala Ala Leu Phe Leu Leu Val 2345 2350 2355 Ala His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr 2360 2365 2370 Arg Glu Ala Gln Lys Arg Ala Ala Ala Gly Ile Met Lys Asn Pro 2375 2380 2385 Thr Val Asp Gly Ile Thr Val Ile Asp Leu Asp Pro Ile Pro Tyr 2390 2395 2400 Asp Pro Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val 2405 2410 2415 Leu Cys Val Thr Gln Val Leu Met Met Arg Thr Thr Trp Ala Leu 2420 2425 2430 Cys Glu Ala Leu Thr Leu Ala Thr Gly Pro Ile Ser Thr Leu Trp 2435 2440 2445 Glu Gly Asn Pro Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser 2450 2455 2460 Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu 2465 2470 2475 Leu Phe Ser Ile Met Lys Asn Thr Thr Asn Thr Arg Arg Gly Thr 2480 2485 2490 Gly Asn Ile Gly Glu Thr Leu Gly Glu Lys Trp Lys Ser Arg Leu 2495 2500 2505 Asn Ala Leu Gly Lys Ser Glu Phe Gln Ile Tyr Lys Lys Ser Gly 2510 2515 2520 Ile Gln Glu Val Asp Arg Thr Leu Ala Lys Glu Gly Ile Lys Arg 2525 2530 2535 Gly Glu Thr Asp His His Ala Val Ser Arg Gly Ser Ala Lys Leu 2540 2545 2550 Arg Trp Phe Val Glu Arg Asn Met Val Thr Pro Glu Gly Lys Val 2555 2560 2565 Val Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Gly 2570 2575 2580 Gly Leu Lys Asn Val Arg Glu Val Lys Gly Leu Thr Lys Gly Gly 2585 2590 2595 Pro Gly His Glu Glu Pro Ile Pro Met Ser Thr Tyr Gly Trp Asn 2600 2605 2610 Leu Val Arg Leu Gln Ser Gly Val Asp Val Phe Phe Ile Pro Pro 2615 2620 2625 Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro 2630 2635 2640 Asn Pro Thr Val Glu Ala Gly Arg Thr Leu Arg Val Leu Asn Leu 2645 2650 2655 Val Glu Asn Trp Leu Asn Asn Asn Thr Gln Phe Cys Ile Lys Val 2660 2665 2670 Leu Asn Pro Tyr Met Pro Ser Val Ile Glu Lys Met Glu Ala Leu 2675 2680 2685 Gln Arg Lys Tyr Gly Gly Ala Leu Val Arg Asn Pro Leu Ser Arg 2690 2695 2700 Asn Ser Thr His Glu Met Tyr Trp Val Ser Asn Ala Ser Gly Asn 2705 2710 2715 Ile Val Ser Ser Val Asn Met Ile Ser Arg Met Leu Ile Asn Arg 2720 2725 2730 Phe Thr Met Arg Tyr Lys Lys Ala Thr Tyr Glu Pro Asp Val Asp 2735 2740 2745 Leu Gly Ser Gly Thr Arg Asn Ile Gly Ile Glu Ser Glu Ile Pro 2750 2755 2760 Asn Leu Asp Ile Ile Gly Lys Arg Ile Glu Lys Ile Lys Gln Glu 2765 2770 2775 His Glu Thr Ser Trp His Tyr Asp Gln Asp His Pro Tyr Lys Thr 2780 2785 2790 Trp Ala Tyr His Gly Ser Tyr Glu Thr Lys Gln Thr Gly Ser Ala 2795 2800 2805 Ser Ser Met Val Asn Gly Val Val Arg Leu Leu Thr Lys Pro Trp 2810 2815 2820 Asp Val Val Pro Met Val Thr Gln Met Ala Met Thr Asp Thr Thr 2825 2830 2835 Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg 2840 2845 2850 Thr Gln Glu Pro Lys Glu Gly Thr Lys Lys Leu Met Lys Ile Thr 2855 2860 2865 Ala Glu Trp Leu Trp Lys Glu Leu Gly Lys Lys Lys Thr Pro Arg 2870 2875 2880 Met Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn Ala 2885 2890 2895 Ala Leu Gly Ala Ile Phe Thr Asp Glu Asn Lys Trp Lys Ser Ala 2900 2905 2910 Arg Glu Ala Val Glu Asp Ser Arg Phe Trp Glu Leu Val Asp Lys 2915 2920 2925 Glu Arg Asn Leu His Leu Glu Gly Lys Cys Glu Thr Cys Val Tyr 2930 2935 2940 Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys 2945 2950 2955 Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg 2960 2965 2970 Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp 2975 2980 2985 Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly Leu 2990 2995 3000 His Lys Leu Gly Tyr Ile Leu Arg Asp Val Ser Lys Lys Glu Gly 3005 3010 3015 Gly Ala Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile 3020 3025 3030 Thr Leu Glu Asp Leu Lys Asn Glu Glu Met Val Thr Asn His Met 3035 3040 3045 Glu Gly Glu His Lys Lys Leu Ala Glu Ala Ile Phe Lys Leu Thr 3050 3055 3060 Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Thr Pro Arg Gly 3065 3070 3075 Thr Val Met Asp Ile Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly 3080 3085 3090 Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala 3095 3100 3105 Gln Leu Ile Arg Gln Met Glu Gly Glu Gly Val Phe Lys Ser Ile 3110 3115 3120 Gln His Leu Thr Ile Thr Glu Glu Ile Ala Val Gln Asn Trp Leu 3125 3130 3135 Ala Arg Val Gly Arg Glu Arg Leu Ser Arg Met Ala Ile Ser Gly 3140 3145 3150 Asp Asp Cys Val Val Lys Pro Leu Asp Asp Arg Phe Ala Ser Ala 3155 3160 3165 Leu Thr Ala Leu Asn Asp Met Gly Lys Ile Arg Lys Asp Ile Gln 3170 3175 3180 Gln Trp Glu Pro Ser Arg Gly Trp Asn Asp Trp Thr Gln Val Pro 3185 3190 3195 Phe Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg 3200 3205 3210 Val Leu Val Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg 3215 3220 3225 Ala Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala 3230 3235 3240 Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe 3245 3250 3255 His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser Ala 3260 3265 3270 Val Pro Ser His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile 3275 3280 3285 His Ala Lys His Glu Trp Met Thr Thr Glu Asp Met Leu Thr Val 3290 3295 3300 Trp Asn Arg Val Trp Ile Gln Glu Asn Pro Trp Met Glu Asp Lys 3305 3310 3315 Thr Pro Val Glu Ser Trp Glu Glu Ile Pro Tyr Leu Gly Lys Arg 3320 3325 3330 Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala 3335 3340 3345 Thr Trp Ala Lys Asn Ile Gln Ala Ala Ile Asn Gln Val Arg Ser 3350 3355 3360 Leu Ile Gly Asn Glu Glu Tyr Thr Asp Tyr Met Pro Ser Met Lys 3365 3370 3375 Arg Phe Arg Arg Glu Glu Glu Glu Ala Gly Val Leu Trp 3380 3385 3390 <210> 9 <211> 10723 <212> DNA <213> Artificial sequence <220> <223> Dengue virus serotype 2, former major <400> 9 agttgttagt ctacgtggac cgacaaagac agattctttg agggagctaa gctcaatgta 60 gttctaacag ttttttaatt agagagcaga tctctgatga ataaccaacg gaaaaaggcg 120 aaaaacacgc ctttcaatat gctgaaacgc gagagaaacc gcgtgtcgac tgtgcaacag 180 ctgacaaaga gattctcact tggaatgctg cagggacgag gaccattaaa actgttcatg 240 gccctggtgg cgttccttcg tttcctaaca atcccaccaa cagcagggat attgaagaga 300 tggggaacaa ttaaaaaatc aaaagctatt aatgttttga gagggttcag gaaagagatt 360 ggaaggatgc tgaacatctt gaataggaga cgcagatctg caggcatgat cattatgctg 420 attccaacag tgatggcgtt ccatttaacc acacgtaacg gagaaccaca catgatcgtc 480 agcagacaag agaaagggaa aagtcttctg tttaaaacag aggttggcgt gaacatgtgt 540 accctcatgg ccatggacct tggtgaattg tgtgaagaca caatcacgta cgagtgtccc 600 cttctcaggc agaatgagcc agaagacata gactgttggt gcaactctac gtccacgtgg 660 gtaacttatg ggacgtgtac caccatggga gaacatagaa gagaaaaaag atcagtggca 720 ctcgttccac atgtgggaat gggactggag acacgaactg aaacatggat gtcatcagaa 780 ggggcctgga aacatgtcca gagaattgaa acttggatct tgagacatcc aggctcacc 840 atgatggcag caatcctggc atacaccata ggaacgacac atttccaag agccctgatc 900 ttcatcttac tgacagctgt cactccttca atgacaatgc gttgcatagg atgtcaaat 960 agagactttg tggaggggt tcaggagga agctggttg acatagtctt agaacatgga 1020 agctgtgtga cgacgatgc aaaaaaaaaaaacattgg attttgaact gataaaaaaca 1080 gagccaaac agcctgccac cctaggaag tactgtatag agggcaagct aaccaaca 1140 acaacagaat ctcgctgccc aacacaaggg gaacccagcc taaatgaga gcaggacaaa 1200 aggttcgtct gcaacactc catggtagac agaggatggg gaatggatg tggactattt 1260 ggaaagggag gcattgtgac ctgtgctatg ttcagatgca aaagaacat ggaaggaaaa 1320 gttgtgcaac cagaaaactt ggaatacacc attgtgataa cacctacactc agggaagg 1380 catgcagtcg gaatgacac aggaaacat ggcaaggaa tcaaataac accacagagt 1440 tccatcacag aagcagaatt ggcactgtca caatggagtg ctctccaaga 1500 acgggcctcg acttcaatga gatggtgttg ctgcagatgg aaaataagc ttggctggtg 1560 cacaggcaat ggttcctaga cctgccgtta ccatggttgc ccggagcgga cacaaggg 1620 tcaaattgga tacagaaaga gacattggtc actttcaaaa atccccatgc gaaaacag 1680 gatgttgttg ttttaggatc ccaagaaggg gccatgcaca cagcacttac aggggccaca 1740 gaaatccaaa tgtcatcagg aaacttactc ttcacaggac atctcaagtg caggctgaga 1800 1860 gtgaaggaaa tagcagaaac acaacatgga acaatagtta tcagagtgca atatgaaggg 1920 gacggctctc catgcaagat cccttttgag ataatggatt tggaaaaaag acatgtctta 1980 ggtcgcctga ttacagtcaa cccaattgtg acagaaaaag atagcccagt caacatagaa 2040 gcagaacctc catttggaga cagctacatc atcatagagg tagagccgggg aaactgaag 2100 ctcaactggt ttaagaaagg aagttctatc ggccaaatgt ttgagacaac aatgaggggg 2160 gcgaagagaa tggccatttt aggtgacaca gcctgggatt ttggatcctt gggaggagtg 2220 tttacatcta taggaagggc tctccaccaa gtctttggag caatctatgg agctgccttc 2280 agtggggttt catggactt gaaaatccctc ataggagtca ttatcacatg gatggaatg 2340 aattcacgca gcacctcact gtctgtgaca ctagtattgg tgggaattgt gatagtat 2400 ttgggagtca tggtgcaggc cgatagtggt tgcgttgtga gctggaaaaa caagaactg 2460 aaatgtggca gtgggattt catcacagac aacgtgcaca catggacaga acatacaag 2520 ttccaaccag aatccccttc aaaactagct tcagctatcc agaagccca tgaagaggac 2580 atttgtggaa tccgctcagt aacagactg gagaatctga tgtggaaca aaacacca 2640 gattgaatc acattctatc agaaaatgag gtgaagttaa ctattatgac aggagacatc 2700 aaaggaatca tgcaggcagg aaacgatct ctgcggccctc agcccactga gctgaagtat 2760 tcatggaaaa catggggcaa agcaaaatg ctctctacag agtctcataa ccagaccttt 2820 ctcattgatg gccccgaac agcagaatgc cccacaca atagagcttg gattcgttg 2880 gaagttgaag actatggctt tggagtattc accaccaata tattggctaaa attgaaagaa 2940 aaaacaggatg tattctgcga ctcaaactc atgtcagcgg ccaaaaga caacagagcc 3000 gtccatgccg attgggtta ttggatagaa agtgcactca atgacacatg gaagatagag 3060 aaagcctctt tcattgaagt taaaaactgc cactggccaa atcacacac cctctggagc 3120 aatggagtgc tagaagtga gatgatatt ccaagaatc tcgctggacc agtgtctca 3180 cacaactata gaccaggcta ccatacacaa atacaggac catggcatct aggtaagctt 3240 gagatgact tgatttctg tgatgacaca acagtgtag tgactgagga ctgcggaat 3300 agaggaccct ctttgagac aaccactgcc tctggaaac tcatacaga atggtgctgc 3360 cgatcttgca cattaccacc gctaagatac agaggtgagg atggttgctg gtacgggatg 3420 gaatcagac cattgaagga gaagagag aatttggtca actccttggt cacagctgga 3480 catgggcagg tcgacaactt ttcactagga gtcttggga tggcattgtt cctggaggaa 3540 atgcttagga cccgagtagg acgaaacat gcaatactac tagttgcagt ttctttgtg 3600 acattgatca cagggaacat gtcctttaga gacctgggaa gagtgatggt tatggtaggc 3660 gccactatga cggatgacat aggtatgggc gtgacttatc ttgcctact agcagccttc 3720 aaagtcagac caacttgc agctggacta ctcttgagaa agctgaccctc caggaattg 3780 atgatgacta ctataggaat tgtactccctc tcccagagca ccataccaga gaccattctt 3840 gagttgactg atgcgttagc cttaggcatg atggtccctca aaatggtgag aaatatgga 3900 aagtatcaat tggcagtgac tatcatggct atcttgtgcg tcccaacgc agtgatatta 3960 caaaacgcat ggaaagtgag ttgcacaata ttggcagtgg tgtccgtttc cccactgttc 4020 ttacatcct cacagcaaa aacagattgg attackcattag cattgacgat caaggctc 4080 aatccaacag ctatttttct aaaaccctc tcaagaacca gcaagaaag gagctggcca 4140 ttaaatgagg ctatcatggc agtcgggatg gtgagcatttt tagccagttc tctcctaaaa 4200 aatgatattc ccatgacagg accattagtg gctggaggc tctcactgt gtgctacgtg 4260 ctcactggac gatcggccga ttggactg gagagagcag ccgatgtca atgggagac 4320 caggcagaga tatcaggaag cagtccaatc ctgtcaataa caatcaga agatggtagc 4380 atgtcgataa aaaatgaaga ggaagaacaa acactgacca tactcattag aacaggattg ctggtgatct caggactttt tcctgtatca attack cggcagcagc atggtacctg tgggaagtga agaaacaacg ggccggagta ttgtgggatg ttccttcacc cccacccatg 4560. ggaaaggctg aactggaaga tggagcctat agaattaagc aaaaagggat tcttggatat tcccagatcg gagccggagt ttacaaagaa ggaacattcc atacaatgtg gcatgtcaca cgtggcgctg ttctaatgca taaaggaaag aggattgaac catcatgggc ggacgtcaag aaagacctaa tatcatatgg aggaggctgg aagttagaag gagaatgga ggaggaga gagtccagg tattggcact gagcctgga aaaaatcca gagccgtcca aacgaaacct ggtcttttca aaaccaacgc cggaacaata ggtgctgtat ctctggactt ttctcctgga 4980. acgtcaggat ctccaattat cgacaaaaaa ggaaaagttg tgggtcttta tggtaatggt gttgttacaa ggagtggagc atatgtgagt gctatagccc agactgaaaa aagcattgaa gacaacccag agatcgaga tgacattttc cgaagaga gactgaccat catggacctc caccaggag cgggaagac gagagatac cttccggcca tagtcagaga agcttaaaa 5160 cggggtttga gacattaat cttggccccc actagagttg tggcagctga aatggaggaa 5220 gcccttagag gacttccaat aagataccag accccagcca tcagagctgt gcacaccggg 5280 cgggagatg tggacctaat gtgtcatgcc accattacca tgaggctgct atcaccagtt 5340 agagtgccaa actacaacct gattatcatg gacgaagccc atttcacaga cccagcaagt 5400 atagcagcta gaggatacat ctcactcga gtggagatgg gtgaggcagc tgggattttt 5460 atgacagcca ctcccccgggg aagcagagac ccattccctc agagcaatgc accaatcata 5520 gatgagaaa gagaatccc tgaacgctcg tggaattccg gatgaatg ggtcacggat 5580 tttaaaggga agactgttg gttcgttcca agtataaaag caggaatga tatagcagct 5640 tgcctgagga aaatggaaa gaagtgata caaccagta ggaagacctt tgattctgag 5700 tatgtcaaga ctagaaccaa tgattgggac ttcgtggtta caactgacat ttcagaatg 5760 ggtgccaatt tcaggctga gagggttata gacccagac gctgcatgaa accagtcata 5820 ctaacagatg gtgaagagcg ggtgattctg gcaggaccta tgccagtgac ccactctagt 5880 gcagcacaaa gaagggag aataggaaga aatccaaaaa atgagaatga ccagtacata 5940 6000 ctcctagata acatcaacac gccagaagga atcattccta gcatgttcga accagagcgt 6060 gaaaaggtgg atgccattga tggcgaatac cgcttgagag gagaagcaag gaaaaccttt 6120 gtagacttaa tgagaagagg agacctacca gtctggttgg cctacagagt ggcagctgaa 6180 ggcatcaact acgcagacag aaggtggtgt tttgatggag tcaagaacaa ccaaatccta 6240 gaaaaacg tggaagttga aatctggaca aaagaagggg aaaggaagaa attgaaaccc 6300 agatggttgg atgctaggat ctattctgac ccactggcgc taaaagaatt taggaattt 6360 gcagccggaa gaaagtctct gaccctgaac ctaatcacag aaatgggtag gctcccaacc 6420 ttcatgactc agaagcaag agacgcactg gacaacttag cagtgctgca cacggctgag 6480 gcaggtggaa gggcgtacaa ccatgctctc agtgaactgc cggagaccct ggagacattg 6540 cttttactga cacttctggc tacagtcacg ggagggatct ttttattctt gatgagcgca 6600 aggggcatag ggaagatgac cctgggaatg tgctgcataa tcacggctag catcctccta 6660 tggtacgcac aaatacagcc acactggata gcagcttcaa taatactgga gttttttctc 6720 atagttttgc ttattccaga acctgaaaaa cagagaacac cccaagacaa ccaactgacc 6780 tacgttgtca tagccatcct cacagtggtg gccgcaacca tggcaaacga gatgggtttc 6840 ctagaaaaaa cgaagaaaga tctcggattg ggaagcattg caacccagca acccgagagc 6900 aacatcctgg acatagatct acgtcctgca tcagcatgga cgctgtatgc cgtggccaca 6960 acatttgtta caccaatgtt gagacatagc attgaaaatt cctcagtgaa tgtgtcccta 7020 acagctatag ccaaccaagc cacagtgtta atgggtctcg ggaaaggatg gccattgtca 7080 aagatggaca tcggagttcc ccttctcgcc attggatgct actcacaagt caaccccata 7140 actctcacag cagctctttt cttattggta gcacattatg ccatcatagg gccaggactc 7200 caagcaaaag caaccagaga agctcagaaa agagcagcgg cgggcatcat gaaaaaccca 7260 actgtcgatg gataacagt gattgaccta gatccaatac cttatgatcc aaagtttgaa 7320 aagcagttgg gatagtaat gctcctagtc ctctgcgtga ctcaagtatt gatgatgagg 7380 actacatggg ctctgtgtga ggctttaacc ttagctaccg ggcccactc cacattgtgg 7440 gaaggaaatc cagggaggtt ttggacact accattgcgg tgtcaatggc tacattttt 7500 agagggagtt acttggccgg agctggactt ctctttcta ttatgagaa cacaccac 7560 acaagaaggg gaactggcaa cataggagag acgcttggag agaatggaa aagccgattg 7620 aacgcattgg gaaaaagtga attccagatc tacaagaaa gtggaatcca ggaagtggat 7680 agaaccttag caaagaagg cattaaaaga ggagaaacgg accacacgc tgtgtcgcga 7740 ggctcagcaa aactgagatg gttcgttgag agaaacatgg tcacaccaga agggaagta 7800 gtggacctcg gttgtggcag aggaggctgg tcatactatt gtggaggact aagaatgta 7860 agagaagtca aaggcctaac aaaaggagga ccaggacacg aagaacccat ccccatgtca 7920 acatagggt ggaatctagt gcgtcttcaa agtggagttg acgttttctt catcccgcca 7980 gaaaagtgtg acacattatt gtgtgacata ggggagtcat caccaaatcc cacagtggaa 8040 gcaggacgaa cactcagagt ccttaactta gtagaaaatt ggttgaacaa caacactcaa 8100 ttttgcataa aggttctcaa cccatatatg ccctcagtca tagaaaaaat ggaagcacta 8160 caaaggaaat atggaggagc cttagtgagg aatccactct cacgaaactc cacacatgag 8220 atgtactggg tatccaatgc ttccgggaac atagtgtcat cagtgaacat gatttcaagg 8280 atgttgatca acagatttac aatgagatac aagaaagcca cttacgagcc ggatgttgac 8340 ctcggaagcg gaacccgtaa catcgggatt gaaagtgaga taccaaacct agatataatt 8400 gggaaaagaa tagaaaaat aaagcaagag catgaaacat catggcacta tgaccaagac 8460 cacccataca aaacgtgggc ataccatggt agctatgaaa caaaacagac tggatcagca 8520 tcatccatgg tcaacggagt ggtcaggctg ctgacaaaac cttgggacgt cgtccccatg 8580 gtgacacaga tggcaatgac agacacgact ccatttggac aacagcgcgt ttttaaagag 8640 aaagtggaca cgagaaccca agaaccgaaa gaaggcacga agaaactaat gaaaataaca 8700 gcagagtggc tttggaaga attaggaag aaaagacac ccaggatgtg caccagagaa 8760 gattcacaa gaaggtgag aagcaatgca gccttgggggg ccgtattcac tgatgagaac 8820 aagtggaagt cggcacgtga ggctgttgaa gatagtaggt tttgggagct ggttgacaag 8880 gaaaggaatc tccatcttga aggaaagtgt gaaacatgtg tgtacaacat gatgggaaaa 8940 aggagaga agctagggga attcggcaag gcaaaggca gcagagccat atggtacatg 9000 tggcttggag cacgcttctt agagtttga gccctaggat tctttaatga agatcactgg 9060 ttctccagag agaactccct gagtggagtg gaaggagaag ggctgcacaa gctaggttac 9120 attctaagag acgtgagcaa gaagaggga ggagcaatgt atgccgatga caccgcagga 9180 tgggatacaa gatcacacact agaagaccta aaaaatgaag aaatggtaac aaaccacatg 9240 gaaggagaac acaagaact agccgaggcc atttcaac taacgtacca aaacaggtg 9300 gtgcgtgtgc aaagaccaac accaagaggc acagtaatgg acatcatatc gagagagac 9360 haaagaggta gtggacaagt tggcacctat ggactcaata ctttcaccaa tatggaagcc 9420 caactaatca gagatgga gggagaagga gtctttaaaa gcattcagca cctaacaatc 9480 acagaagaaa tcgctgtgca aactggtta gcaagagtgg ggcgcgaaag gttatcaaga 9540 atggccatca gtggagatga ttgtgttgtg aaacctttag atgacaggtt cgcaagcgct 9600 ttaacagctc taaatgacat gggaagatt aggaagaca tacacaatg ggaaccttca 9660 agaggatgga atgattggac acaagtgccc ttctgttcac accattcca tgagttaatc 9720 atgaaagacg gtcgcgtact cgttgttcca tgtagaaacc aagatgaact gattggcaga 9780 gcccgaatct cccaaggagc agggtggtct ttgcgggaga cggcctgtttt ggggaagtct 9840 tacgcccaaa tgtggagctt gatgtacttc cacagacgcg acctcaggct ggcggcaaat 9900 gctatttgct cggcagtacc atcacattgg gttccacaa gtcgacaac ctggtccata 9960 catgctaaac atgaatggat vakaacggaa gatatgctga cagtctggaa cagggtgtgg 10020 attcaagaaa acccatggat ggaagacaaa actccagtgg aatcatggga ggaatccca 10080 tacttgggga aaagagaga ccaatggtgc ggctcattga ttggttaac aagcaggggcc 10140 acctgggcaa agaacatcca agcagcaata aatcaagtta gatcccttat aggcaatgaa 10200 gaatacacag attacatgcc atccatgaaa agattcagaa gagaagagga agaagcagga 10260 gttctgtggt agaaagcaaa actaacatga aacaaggcta gaagtcaggt cggattaagc 10320 catagtacgg aaaaaactat gctacctgtg agccccgtcc aaggacgtta aaagaagtca 10380 ggccatcata aatgccatag cttgagtaaa ctatgcagcc tgtagctcca cctgagaagg 10440 tgtaaaaaat ccgggaggcc acaaaccatg gaagctgtac gcatggcgta gtggactagc 10500 ggttagagga gacccctccc ttacaaatcg cagcaacaat gggggcccaa ggcgagatga 10560 agctgtagtc tcgctggaag gactagaggt tagaggagac ccccccgaaa caaaaaacag 10620 catattgacg ctgggaaaga ccagagatcc tgctgtctcc tcagcatcat tccaggcaca 10680 gaacgccaga aaatggaatg gtgctgttga atcaacaggt tct 10723 <210>10 <211>3391 <212>PRT <213>Artificial Sequence <220> <223>Dengue virus serotype 2, pre-major <400>10 Met Asn Asn Gln Arg Lys Lys Ala Lys Asn Thr Pro Phe Asn Met Leu 1 5 10 15 Lys Arg Glu Arg Asn Arg Val Ser Thr Val Gln Gln Leu Thr Lys Arg 20 25 30 Phe Ser Leu Gly Met Leu Gln Gly Arg Gly Pro Leu Lys Leu Phe Met 35 40 45 Ala Leu Val Ala Phe Leu Arg Phe Leu Thr Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Lys Arg Trp Gly Thr Ile Lys Lys Ser Lys Ala Ile Asn Val 65 70 75 80 Leu Arg Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn 85 90 95 Arg Arg Arg Arg Ser Ala Gly Met Ile Ile Met Leu Ile Pro Thr Val 100 105 110 Met Ala Phe His Leu Thr Thr Arg Asn Gly Glu Pro His Met Ile Val 115 120 125 Ser Arg Gln Glu Lys Gly Lys Ser Leu Leu Phe Lys Thr Glu Val Gly 130 135 140 Val Asn Met Cys Thr Leu Met Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Ile Thr Tyr Glu Cys Pro Leu Leu Arg Gln Asn Glu Pro Glu 165 170 175 Asp Ile Asp Cys Trp Cys Asn Ser Thr Ser Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Thr Thr Met Gly Glu His Arg Arg Glu Lys Arg Ser Val Ala 195 200 205 Leu Val Pro His Val Gly Met Gly Leu Glu Thr Arg Thr Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys His Val Gln Arg Ile Glu Thr Trp 225 230 235 240 Ile Leu Arg His Pro Gly Phe Thr Met Met Ala Ala Ile Leu Ala Tyr 245 250 255 Thr Ile Gly Thr Thr His Phe Gln Arg Ala Leu Ile Phe Ile Leu Leu 260 265 270 Thr Ala Val Thr Pro Ser Met Thr Met Arg Cys Ile Gly Met Ser Asn 275 280 285 Arg Asp Phe Val Glu Gly Val Ser Gly Gly Ser Trp Val Asp Ile Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Phe Glu Leu Ile Lys Thr Glu Ala Lys Gln Pro Ala Thr Leu 325 330 335 Arg Lys Tyr Cys Ile Glu Ala Lys Leu Thr Asn Thr Thr Thr Glu Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Pro Ser Leu Asn Glu Glu Gln Asp Lys 355 360 365 Arg Phe Val Cys Lys His Ser Met Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Gly Ile Val Thr Cys Ala Met Phe Arg 385 390 395 400 Cys Lys Lys Asn Met Glu Gly Lys Val Val Gln Pro Glu Asn Leu Glu 405 410 415 Tyr Thr Ile Val Ile Thr Pro His Ser Gly Glu Glu His Ala Val Gly 420 425 430 Asn Asp Thr Gly Lys His Gly Lys Glu Ile Lys Ile Thr Pro Gln Ser 435 440 445 Ser Ile Thr Glu Ala Glu Leu Thr Gly Tyr Gly Thr Val Thr Met Glu 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Gln 465 470 475 480 Met Glu Asn Lys Ala Trp Leu Val His Arg Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Leu Pro Gly Ala Asp Thr Gln Gly Ser Asn Trp Ile 500 505 510 Gln Lys Glu Thr Leu Val Thr Phe Lys Asn Pro His Ala Lys Lys Gln 515 520 525 Asp Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Met Ser Ser Gly Asn Leu Leu Phe Thr 545 550 555 560 Gly His Leu Lys Cys Arg Leu Arg Met Asp Lys Leu Gln Leu Lys Gly 565 570 575 Met Ser Tyr Ser Met Cys Thr Gly Lys Phe Lys Val Val Lys Glu Ile 580 585 590 Ala Glu Thr Gln His Gly Thr Ile Val Ile Arg Val Gln Tyr Glu Gly 595 600 605 Asp Gly Ser Pro Cys Lys Ile Pro Phe Glu Ile Met Asp Leu Glu Lys 610 615 620 Arg His Val Leu Gly Arg Leu Ile Thr Val Asn Pro Ile Val Thr Glu 625 630 635 640 Lys Asp Ser Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Asp Ser 645 650 655 Tyr Ile Ile Ile Gly Val Glu Pro Gly Gln Leu Lys Leu Asn Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Gln Met Phe Glu Thr Thr Met Arg Gly 675 680 685 Ala Lys Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Leu Gly Gly Val Phe Thr Ser Ile Gly Lys Ala Leu His Gln Val Phe 705 710 715 720 Gly Ala Ile Tyr Gly Ala Ala Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Leu Ile Gly Val Ile Ile Thr Trp Ile Gly Met Asn Ser Arg Ser 740 745 750 Thr Ser Leu Ser Val Thr Leu Val Leu Val Gly Ile Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Val Ser Trp Lys 770 775 780 Asn Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Ile Thr Asp Asn Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ser Lys 805 810 815 Leu Ala Ser Ala Ile Gln Lys Ala His Glu Glu Asp Ile Cys Gly Ile 820 825 830 Arg Ser Val Thr Arg Leu Glu Asn Leu Met Trp Lys Gln Ile Thr Pro 835 840 845 Glu Leu Asn His Ile Leu Ser Glu Asn Glu Val Lys Leu Thr Ile Met 850 855 860 Thr Gly Asp Ile Lys Gly Ile Met Gln Ala Gly Lys Arg Ser Leu Arg 865 870 875 880 Pro Gln Pro Thr Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala 885 890 895 Lys Met Leu Ser Thr Glu Ser His Asn Gln Thr Phe Leu Ile Asp Gly 900 905 910 Pro Glu Thr Ala Glu Cys Pro Asn Thr Asn Arg Ala Trp Asn Ser Leu 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Lys Glu Lys Gln Asp Val Phe Cys Asp Ser Lys Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Asn Arg Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Ala Leu Asn Asp Thr Trp Lys Ile Glu Lys Ala Ser Phe 980 985 990 Ile Glu Val Lys Asn Cys His Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Asn Leu Ala 1010 1015 1020 Gly Pro Val Ser Gln His Asn Tyr Arg Pro Gly Tyr His Thr Gln 1025 1030 1035 Ile Thr Gly Pro Trp His Leu Gly Lys Leu Glu Met Asp Phe Asp 1040 1045 1050 Phe Cys Asp Gly Thr Thr Val Val Val Thr Glu Asp Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Ile 1070 1075 1080 Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr 1085 1090 1095 Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala Gly 1115 1120 1125 His Gly Gln Val Asp Asn Phe Ser Leu Gly Val Leu Gly Met Ala 1130 1135 1140 Leu Phe Leu Glu Glu Met Leu Arg Thr Arg Val Gly Thr Lys His 1145 1150 1155 Ala Ile Leu Leu Val Ala Val Ser Phe Val Thr Leu Ile Thr Gly 1160 1165 1170 Asn Met Ser Phe Arg Asp Leu Gly Arg Val Met Val Met Val Gly 1175 1180 1185 Ala Thr Met Thr Asp Asp Ile Gly Met Gly Val Thr Tyr Leu Ala 1190 1195 1200 Leu Leu Ala Ala Phe Lys Val Arg Pro Thr Phe Ala Ala Gly Leu 1205 1210 1215 Leu Leu Arg Lys Leu Thr Ser Lys Glu Leu Met Met Thr Thr Ile 1220 1225 1230 Gly Ile Val Leu Leu Ser Gln Ser Thr Ile Pro Glu Thr Ile Leu 1235 1240 1245 Glu Leu Thr Asp Ala Leu Ala Leu Gly Met Met Val Leu Lys Met 1250 1255 1260 Val Arg Asn Met Glu Lys Tyr Gln Leu Ala Val Thr Ile Met Ala 1265 1270 1275 Ile Leu Cys Val Pro Asn Ala Val Ile Leu Gln Asn Ala Trp Lys 1280 1285 1290 Val Ser Cys Thr Ile Leu Ala Val Val Ser Val Ser Pro Leu Phe 1295 1300 1305 Leu Thr Ser Ser Gln Gln Lys Thr Asp Trp Ile Pro Leu Ala Leu 1310 1315 1320 Thr Ile Lys Gly Leu Asn Pro Thr Ala Ile Phe Leu Thr Thr Leu 1325 1330 1335 Ser Arg Thr Ser Lys Lys Arg Ser Trp Pro Leu Asn Glu Ala Ile 1340 1345 1350 Met Ala Val Gly Met Val Ser Ile Leu Ala Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Ile Pro Met Thr Gly Pro Leu Val Ala Gly Gly Leu Leu 1370 1375 1380 Thr Val Cys Tyr Val Leu Thr Gly Arg Ser Ala Asp Leu Glu Leu 1385 1390 1395 Glu Arg Ala Ala Asp Val Lys Trp Glu Asp Gln Ala Glu Ile Ser 1400 1405 1410 Gly Ser Ser Pro Ile Leu Ser Ile Thr Ile Ser Glu Asp Gly Ser 1415 1420 1425 Met Ser Ile Lys Asn Glu Glu Glu Glu Gln Thr Leu Thr Ile Leu 1430 1435 1440 Ile Arg Thr Gly Leu Leu Val Ile Ser Gly Leu Phe Pro Val Ser 1445 1450 1455 Ile Pro Ile Thr Ala Ala Ala Trp Tyr Leu Trp Glu Val Lys Lys 1460 1465 1470 Gln Arg Ala Gly Val Leu Trp Asp Val Pro Ser Pro Pro Pro Met 1475 1480 1485 Gly Lys Ala Glu Leu Glu Asp Gly Ala Tyr Arg Ile Lys Gln Lys 1490 1495 1500 Gly Ile Leu Gly Tyr Ser Gln Ile Gly Ala Gly Val Tyr Lys Glu 1505 1510 1515 Gly Thr Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met His Lys Gly Lys Arg Ile Glu Pro Ser Trp Ala Asp Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Lys Leu Glu Gly Glu 1550 1555 1560 Trp Lys Glu Gly Glu Glu Val Gln Val Leu Ala Leu Glu Pro Gly 1565 1570 1575 Lys Asn Pro Arg Ala Val Gln Thr Lys Pro Gly Leu Phe Lys Thr 1580 1585 1590 Asn Ala Gly Thr Ile Gly Ala Val Ser Leu Asp Phe Ser Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Ile Asp Lys Lys Gly Lys Val Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Arg Ser Gly Ala Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Thr Glu Lys Ser Ile Glu Asp Asn Pro Glu Ile 1640 1645 1650 Glu Asp Asp Ile Phe Arg Lys Arg Arg Leu Thr Ile Met Asp Leu 1655 1660 1665 His Pro Gly Ala Gly Lys Thr Lys Arg Tyr Leu Pro Ala Ile Val 1670 1675 1680 Arg Glu Ala Ile Lys Arg Gly Leu Arg Thr Leu Ile Leu Ala Pro 1685 1690 1695 Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu 1700 1705 1710 Pro Ile Arg Tyr Gln Thr Pro Ala Ile Arg Ala Val His Thr Gly 1715 1720 1725 Arg Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg 1730 1735 1740 Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met 1745 1750 1755 Asp Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly 1760 1765 1770 Tyr Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Gly Ile Phe 1775 1780 1785 Met Thr Ala Thr Pro Pro Gly Ser Arg Asp Pro Phe Pro Gln Ser 1790 1795 1800 Asn Ala Pro Ile Ile Asp Glu Glu Arg Glu Ile Pro Glu Arg Ser 1805 1810 1815 Trp Asn Ser Gly His Glu Trp Val Thr Asp Phe Lys Gly Lys Thr 1820 1825 1830 Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Ala 1835 1840 1845 Cys Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys 1850 1855 1860 Thr Phe Asp Ser Glu Tyr Val Lys Thr Arg Thr Asn Asp Trp Asp 1865 1870 1875 Phe Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys 1880 1885 1890 Ala Glu Arg Val Ile Asp Pro Arg Arg Cys Met Lys Pro Val Ile 1895 1900 1905 Leu Thr Asp Gly Glu Glu Arg Val Ile Leu Ala Gly Pro Met Pro 1910 1915 1920 Val Thr His Ser Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg 1925 1930 1935 Asn Pro Lys Asn Glu Asn Asp Gln Tyr Ile Tyr Met Gly Glu Pro 1940 1945 1950 Leu Glu Asn Asp Glu Asp Cys Ala His Trp Lys Glu Ala Lys Met 1955 1960 1965 Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ser Met 1970 1975 1980 Phe Glu Pro Glu Arg Glu Lys Val Asp Ala Ile Asp Gly Glu Tyr 1985 1990 1995 Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Asp Leu Met Arg 2000 2005 2010 Arg Gly Asp Leu Pro ValTrp Leu Ala Tyr Arg Val Ala Ala Glu 2015 2020 2025 Gly Ile Asn Tyr Ala Asp Arg Arg Trp Cys Phe Asp Gly Val Lys 2030 2035 2040 Asn Asn Gln Ile Leu Glu Glu Asn Val Glu Val Glu Ile Trp Thr 2045 2050 2055 Lys Glu Gly Glu Arg Lys Lys Leu Lys Pro Arg Trp Leu Asp Ala 2060 2065 2070 Arg Ile Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Glu Phe 2075 2080 2085 Ala Ala Gly Arg Lys Ser Leu Thr Leu Asn Leu Ile Thr Glu Met 2090 2095 2100 Gly Arg Leu Pro Thr Phe Met Thr Gln Lys Ala Arg Asp Ala Leu 2105 2110 2115 Asp Asn Leu Ala Val Leu His Thr Ala Glu Ala Gly Gly Arg Ala 2120 2125 2130 Tyr Asn His Ala Leu Ser Glu Leu Pro Glu Thr Leu Glu Thr Leu 2135 2140 2145 Leu Leu Leu Thr Leu Leu Ala Thr Val Thr Gly Gly Ile Phe Leu 2150 2155 2160 Phe Leu Met Ser Ala Arg Gly Ile Gly Lys Met Thr Leu Gly Met 2165 2170 2175 Cys Cys Ile Ile Thr Ala Ser Ile Leu Leu Trp Tyr Ala Gln Ile 2180 2185 2190 Gln Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu 2195 2200 2205 Ile Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln 2210 2215 2220 Asp Asn Gln Leu Thr Tyr Val Val Ile Ala Ile Leu Thr Val Val 2225 2230 2235 Ala Ala Thr Met Ala Asn Glu Met Gly Phe Leu Glu Lys Thr Lys 2240 2245 2250 Lys Asp Leu Gly Leu Gly Ser Ile Ala Thr Gln Gln Pro Glu Ser 2255 2260 2265 Asn Ile Leu Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu 2270 2275 2280 Tyr Ala Val Ala Thr Thr Phe Val Thr Pro Met Leu Arg His Ser 2285 2290 2295 Ile Glu Asn Ser Ser Val Asn Val Ser Leu Thr Ala Ile Ala Asn 2300 2305 2310 Gln Ala Thr Val Leu Met Gly Leu Gly Lys Gly Trp Pro Leu Ser 2315 2320 2325 Lys Met Asp Ile Gly Val Pro Leu Leu Ala Ile Gly Cys Tyr Ser 2330 2335 2340 Gln Val Asn Pro Ile Thr Leu Thr Ala Ala Leu Phe Leu Leu Val 2345 2350 2355 Ala His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr 2360 2365 2370 Arg Glu Ala Gln Lys Arg Ala Ala Ala Gly Ile Met Lys Asn Pro 2375 2380 2385 Thr Val Asp Gly Ile Thr Val Ile Asp Leu Asp Pro Ile Pro Tyr 2390 2395 2400 Asp Pro Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val 2405 2410 2415 Leu Cys Val Thr Gln Val Leu Met Met Arg Thr Thr Trp Ala Leu 2420 2425 2430 Cys Glu Ala Leu Thr Leu Ala Thr Gly Pro Ile Ser Thr Leu Trp 2435 2440 2445 Glu Gly Asn Pro Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser 2450 2455 2460 Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu 2465 2470 2475 Leu Phe Ser Ile Met Lys Asn Thr Thr Asn Thr Arg Arg Gly Thr 2480 2485 2490 Gly Asn Ile Gly Glu Thr Leu Gly Glu Lys Trp Lys Ser Arg Leu 2495 2500 2505 Asn Ala Leu Gly Lys Ser Glu Phe Gln Ile Tyr Lys Lys Ser Gly 2510 2515 2520 Ile Gln Glu Val Asp Arg Thr Leu Ala Lys Glu Gly Ile Lys Arg 2525 2530 2535 Gly Glu Thr Asp His His Ala Val Ser Arg Gly Ser Ala Lys Leu 2540 2545 2550 Arg Trp Phe Val Glu Arg Asn Met Val Thr Pro Glu Gly Lys Val 2555 2560 2565 Val Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Gly 2570 2575 2580 Gly Leu Lys Asn Val Arg Glu Val Lys Gly Leu Thr Lys Gly Gly 2585 2590 2595 Pro Gly His Glu Glu Pro Ile Pro Met Ser Thr Tyr Gly Trp Asn 2600 2605 2610 Leu Val Arg Leu Gln Ser Gly Val Asp Val Phe Phe Ile Pro Pro 2615 2620 2625 Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro 2630 2635 2640 Asn Pro Thr Val Glu Ala Gly Arg Thr Leu Arg Val Leu Asn Leu 2645 2650 2655 Val Glu Asn Trp Leu Asn Asn Asn Thr Gln Phe Cys Ile Lys Val 2660 2665 2670 Leu Asn Pro Tyr Met Pro Ser Val Ile Glu Lys Met Glu Ala Leu 2675 2680 2685 Gln Arg Lys Tyr Gly Gly Ala Leu Val Arg Asn Pro Leu Ser Arg 2690 2695 2700 Asn Ser Thr His Glu Met Tyr Trp Val Ser Asn Ala Ser Gly Asn 2705 2710 2715 Ile Val Ser Ser Val Asn Met Ile Ser Arg Met Leu Ile Asn Arg 2720 2725 2730 Phe Thr Met Arg Tyr Lys Lys Ala Thr Tyr Glu Pro Asp Val Asp 2735 2740 2745 Leu Gly Ser Gly Thr Arg Asn Ile Gly Ile Glu Ser Glu Ile Pro 2750 2755 2760 Asn Leu Asp Ile Ile Gly Lys Arg Ile Glu Lys Ile Lys Gln Glu 2765 2770 2775 His Glu Thr Ser Trp His Tyr Asp Gln Asp His Pro Tyr Lys Thr 2780 2785 2790 Trp Ala Tyr His Gly Ser Tyr Glu Thr Lys Gln Thr Gly Ser Ala 2795 2800 2805 Ser Ser Met Val Asn Gly Val Val Arg Leu Leu Thr Lys Pro Trp 2810 2815 2820 Asp Val Val Pro Met Val Thr Gln Met Ala Met Thr Asp Thr Thr 2825 2830 2835 Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg 2840 2845 2850 Thr Gln Glu Pro Lys Glu Gly Thr Lys Lys Leu Met Lys Ile Thr 2855 2860 2865 Ala Glu Trp Leu Trp Lys Glu Leu Gly Lys Lys Lys Thr Pro Arg 2870 2875 2880 Met Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn Ala 2885 2890 2895 Ala Leu Gly Ala Val Phe Thr Asp Glu Asn Lys Trp Lys Ser Ala 2900 2905 2910 Arg Glu Ala Val Glu Asp Ser Arg Phe Trp Glu Leu Val Asp Lys 2915 2920 2925 Glu Arg Asn Leu His Leu Glu Gly Lys Cys Glu Thr Cys Val Tyr 2930 2935 2940 Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys 2945 2950 2955 Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg 2960 2965 2970 Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp 2975 2980 2985 Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly Leu 2990 2995 3000 His Lys Leu Gly Tyr Ile Leu Arg Asp Val Ser Lys Lys Glu Gly 3005 3010 3015 Gly Ala Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile 3020 3025 3030 Thr Leu Glu Asp Leu Lys Asn Glu Glu Met Val Thr Asn His Met 3035 3040 3045 Glu Gly Glu His Lys Lys Leu Ala Glu Ala Ile Phe Lys Leu Thr 3050 3055 3060 Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Thr Pro Arg Gly 3065 3070 3075 Thr Val Met Asp Ile Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly 3080 3085 3090 Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala 3095 3100 3105 Gln Leu Ile Arg Gln Met Glu Gly Glu Gly Val Phe Lys Ser Ile 3110 3115 3120 Gln His Leu Thr Ile Thr Glu Glu Ile Ala Val Gln Asn Trp Leu 3125 3130 3135 Ala Arg Val Gly Arg Glu Arg Leu Ser Arg Met Ala Ile Ser Gly 3140 3145 3150 Asp Asp Cys Val Val Lys Pro Leu Asp Asp Arg Phe Ala Ser Ala 3155 3160 3165 Leu Thr Ala Leu Asn Asp Met Gly Lys Ile Arg Lys Asp Ile Gln 3170 3175 3180 Gln Trp Glu Pro Ser Arg Gly Trp Asn Asp Trp Thr Gln Val Pro 3185 3190 3195 Phe Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg 3200 3205 3210 Val Leu Val Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg 3215 3220 3225 Ala Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala 3230 3235 3240 Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe 3245 3250 3255 His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser Ala 3260 3265 3270 Val Pro Ser His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile 3275 3280 3285 His Ala Lys His Glu Trp Met Thr Thr Glu Asp Met Leu Thr Val 3290 3295 3300 Trp Asn Arg Val Trp Ile Gln Glu Asn Pro Trp Met Glu Asp Lys 3305 3310 3315 Thr Pro Val Glu Ser Trp Glu Glu Ile Pro Tyr Leu Gly Lys Arg 3320 3325 3330 Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala 3335 3340 3345 Thr Trp Ala Lys Asn Ile Gln Ala Ala Ile Asn Gln Val Arg Ser 3350 3355 3360 Leu Ile Gly Asn Glu Glu Tyr Thr Asp Tyr Met Pro Ser Met Lys 3365 3370 3375 Arg Phe Arg Arg Glu Glu Glu Glu Ala Gly Val Leu Trp 3380 3385 3390 <210> 11 <211> 10723 <212> DNA <213> artificial sequence <220> <223> Dengue virus serotype 2, MVS <400> 11 agttgttagt ctacgtggac cgacaaagac agattctttg agggagctaa gctcaatgta 60 gttctaacag ttttttaatt agagacaga tctctgatga ataaccaacg gaaaaaggcg 120 aaaaaacacgc ctttcaatat gctgaaacgc gagagaaacc gcgtgtcgac tgtgcaacag 180 ctgacaaaga gattctcact tggaatgctg cagggacgag gaccattaaa actgttcatg 240 gccctggtgg cgttccttcg tttcctaaca atcccaccaa cagcagggat attgaagaga 300 tggggaaacaa ttaaaaaatc aaaagctatt aatgttttga gagggttcag gaaagagatt 360 ggaaggatgc tgaacatctt gaataggaga cgcagatctg caggcatgat cattatgctg 420 attccaacag tgatggcgtt ccatttaacc acacgtaacg gagaaccaca catgatcgtc 480 agcagacaag agaaagggaa aagtcttctg tttaaaacag aggttggcgt gaacatgtgt 540 accctcatgg ccatggacct tggtgaattg tgtgaagaca caatcacgta cgagtgtccc 600 cttctcaggc agaatgagcc agaagacata gactgttggt gcaacctac gtccacgtgg 660 gtaacttatg ggacgtgtac caccatggga gaacatagaa gagaaaaaag atcagtggca 720 ctcgttccac atgtgggaat gggactggag acacgaactg aaacatggat gtcatcagaa 780 ggggcctgga aacatgtcca gagaattgaa acttggatct tgagacatcc aggcttcacc 840 atgatggcag caatcctggc atacaccata ggaacgacac atttccaaag agccctgatc 900 ttcatcttac tgacagctgt cactccttca atgacaatgc gttgcatagg aatgtcaaat 960 agagactttg tggaaggggt ttcaggagga agctgggttg acatagtctt agaacatgga 1020 agctgtgtga cgacgatggc aaaaaacaaa ccaacattgg attttgaact gataaaaaca 1080 gagccaaac agcctgccac cctaggaag tactgtatag agggcaagct aaccaaca 1140 acaacagaat ctcgctgccc aacacaaggg gaacccagcc taaatgaga gcaggacaaa 1200 aggttcgtct gcaacactc catggtagac agaggatggg gaatggatg tggactattt 1260 ggaaagggag gcattgtgac ctgtgctatg ttcagatgca aaagaacat ggaaggaaaa 1320 gttgtgcaac cagaaaactt ggaatacacc attgtgataa cacctacactc agggaagg 1380 catgcagtcg gaatgacac aggaaacat ggcaaggaa tcaaataac accacagagt 1440 tccatcacag aagcagaatt ggcactgtca caatggagtg ctctccaaga 1500 acggggcctcg acttcaatga gatggtgttg ctgcagatgg aaataagc ttggctggtg 1560 cacaggcaat ggttcctaga cctgccgtta ccatggttgc ccggagcgga cacacaaggg 1620 tcaattgga tacagaaga gatattggtc actttcaaa atccccatgc gagaacag 1680 gatgttgttg ttttaggatc ccaagaaggg gccatgcaca cagcacttac aggggccaca 1740 gaaatccaaa tgtcatcagg aaacttactc ttcacaggac atctcaagtg caggctgaga 1800 1860 gtgaaggaaa tagcagaaac acaacatgga acaatagtta tcagagtgca atatgaaggg 1920 gacggctctc catgcaagat cccttttgag ataatggatt tggaaaaaag acatgtctta 1980 ggtcgcctga ttacagtcaa cccaattgtg acagaaaaag atagcccagt caacatagaa 2040 gcagaacctc catttggaga cagctacatc atcatagagg tagagccgggg aaactgaag 2100 ctcaactggt ttaagaaagg aagttctatc ggccaaatgt ttgagacaac aatgaggggg 2160 gcgaagagaa tggccatttt aggtgacaca gcctgggatt ttggatcctt gggaggagtg 2220 tttacatcta taggaaaggc tctccaccaa gtctttggag caatctatgg agctgccttc 2280 agtggggttt catggactat gaaaatcctc ataggagtca ttatcacatg gataggaatg 2340 aattcacgca gcacctcact gtctgtgaca ctagtattgg tgggaattgt gacactgtat 2400 ttgggagtca tggtgcaggc cgatagtggt tgcgttgtga gctggaaaaa caaagaactg 2460 aaatgtggca gtgggatttt catcacagac aacgtgcaca catggacaga acaatacaag 2520 ttccaaccag aatccccttc aaaactagct tcagctatcc agaagccca tgaagaggac 2580 atttgtggaa tccgctcagt aacagactg gagaatctga tgtggaaca aaacacca 2640 gattgaatc acattctatc agaaaatgag gtgaagttaa ctattatgac aggagacatc 2700 aaaggaatca tgcaggcagg aaacgatct ctgcggccctc agcccactga gctgaagtat 2760 tcatggaaaa catggggcaa agcaaaatg ctctctacag agtctcataa ccagaccttt 2820 ctcattgatg gccccgaac agcagaatgc cccacaca atagagcttg gattcgttg 2880 gaagttgaag actatggctt tggagtattc accaccaata tattggctaaa attgaaagaa 2940 aaaacaggatg tattctgcga ctcaaactc atgtcagcgg ccaaaaga caacagagcc 3000 gtccatgccg attgggtta ttggatagaa agtgcactca atgacacatg gaagatagag 3060 aaagcctctt tcattgaagt taaaaactgc cactggccaa atcacacac cctctggagc 3120 aatggagtgc tagaagtga gatgatatt ccaagaatc tcgctggacc agtgtctca 3180 cacaactata gaccaggcta ccatacacaa atacaggac catggcatct aggtaagctt 3240 gagatgact tgatttctg tgatgacaca acagtgtag tgactgagga ctgcggaat 3300 agaggaccct ctttgagac aaccactgcc tctggaaac tcatacaga atggtgctgc 3360 cgatcttgca cattaccacc gctaagatac agaggtgagg atggttgctg gtacgggatg 3420 gaatcagac cattgaagga gaagagag aatttggtca actccttggt cacagctgga 3480 catgggcagg tcgacaactt ttcactagga gtcttggga tggcattgtt cctggaggaa 3540 atgcttagga cccgagtagg acgaaacat gcaatactac tagttgcagt ttctttgtg 3600 acattgatca cagggaacat gtcctttaga gacctgggaa gagtgatggt tatggtaggc 3660 gccactatga cggatgacat aggtatgggc gtgacttatc ttgcctact agcagccttc 3720 aaagtcagac caacttgc agctggacta ctcttgagaa agctgaccctc caggaattg 3780 atgatgacta ctataggaat tgtactccctc tcccagagca ccataccaga gaccattctt 3840 gagttgactg atgcgttagc cttaggcatg atggtccctca aaatggtgag aaatatgga 3900 aagtatcaat tggcagtgac tatcatggct atcttgtgcg tcccaacgc agtgatatta 3960 caaaacgcat ggaaagtgag ttgcacaata ttggcagtgg tgtccgtttc cccactgttc 4020 ttacatcct cacagcaaa aacagattgg attackcattag cattgacgat caaggctc 4080 aatccaacag ctatttttct aaaaccctc tcaagaacca gcaagaaag gagctggcca 4140 ttaaatgagg ctatcatggc agtcgggatg gtgagcatttt tagccagttc tctcctaaaa 4200 aatgatattc ccatgacagg accattagtg gctggaggc tctcactgt gtgctacgtg 4260 ctcactggac gatcggccga ttggactg gagagagcag ccgatgtca atgggagac 4320 caggcagaga tatcaggaag cagtccaatc ctgtcaataa caatcaga agatggtagc 4380 atgtcgataa aaatgaaga ggagaacaa acactgacca tactcattag aacaggattg 4440 ctggtgatct caggacttt tctgtatca ataccaatca cggcagcagc atggtacctg 4500 tgggaagtga agaaacaacg ggccggagta tgtgggatg ttccttcacc cccacccatg 4560 ggaaaggctg aactggaga tggactt agattaagc aaaagggat tcttggatat 4620 tcccagatcg gagccggagt ttacaagaa ggaacattcc atacaatgtg gcatgtcaca 4680 cgtggcgctg ttctaatgca taaggaaag aggattgaac catcatgggc ggacgtcaag 4740 aagacctaa tatcatatgg aggaggctgg aagttagaag gagaatgaa ggaggagaa 4800 gaagtccagg tattggcact ggagcctgga aaaaatccaa gagccgtcca aacgaaacct 4860 ggtctttca aaaccaacgc cggaacaata ggtgctgtat ctctggactt ttctcctgga 4920 acgtcaggat ctccaattat cgacaaaaa ggaaagttg tggtcttta tggtaatggt 4980 gttgttacaa ggagtggagc atatgtgagt gctatagccc agactgaaa aagcattgaa 5040 gaacccag agatcgaga tgacattttc cgaaagagaa gactgaccat catggaccctc 5100 cacccaggag cgggaagac gagagatac cttccggcca tagtcagaga agcttaaaa 5160 cggggtttga gacattaat cttggccccc actagagttg tggcagctga aatggaggaa 5220 gcccttagag gacttccaat aagataccag accccagcca tcagagctgt gcacaccggg 5280 cgggagatg tggacctaat gtgtcatgcc accattacca tgaggctgct atcaccagtt 5340 agagtgccaa actacaacct gattatcatg gacgaagccc atttcacaga cccagcaagt 5400 atagcagcta gaggatacat ctcaactcga gtggagatgg gtgaggcagc tgggatttt atgacagcca ctccccggg aagcagagac ccattttcctc aagcaatgc accaatcata gatgaagaaa gagaaatccc tgaacgctcg tggaattccg gacatgaatg ggtcacggat tttaaaggga agactgtttg gttcgttcca agtataaaag caggaaatga tatagcagct tgcctgagga aaaatggaaa gaaagtgata caactcagta ggaagacctt tgattctgag tatgtcaaga ctagaaccaa tgattgggac ttcgtggtta caactgacat ttcagaaatg ggtgccaatt tcaaggctga gagggttata gaccccagac gctgcatgaa accagtcata ctaacagatg gtgaagagcg ggtgattctg gcaggaccta tgccagtgac ccactctagt gcagcacaaa gaagagggag aataggaaga aatccaaaaa atgagaatga ccagtacata tacatggggg aacctctgga aaatgatgaa gactgtgcac actggaaaga agctaaaatg ctcctagata acatcaacac gccagaagga atcattccta gcatgttcga accagagcgt gaaaggtgg atgccattga tggcgaatac cgcttgagag gagaagcaag gaaaaaccttt gtagacttaa tgagaagagg agacctacca gtctggttgg cctacagagt ggcagctgaa 6180 ggcatcaact acgcagacag aaggtggtgt tttgatggag tcaagaacaa ccaaatccta 6240 gaaaaacg tggaagttga aatctggaca aaagaagggg aaaggaagaa attgaaaccc 6300 agatggttgg atgctaggat ctattctgac ccactggcgc taaaagaatt taggaattt 6360 gcagccggaa gaaagtctct gaccctgaac ctaatcacag aaatgggtag gctcccaacc 6420 ttcatgactc agaagcaag agacgcactg gacaacttag cagtgctgca cacggctgag 6480 gcaggtggaa gggcgtacaa ccatgctctc agtgaactgc cggagaccct ggagacattg 6540 cttttactga cacttctggc tacagtcacg ggagggatct ttttattctt gatgagcgca 6600 aggggcatag ggaagatgac cctgggaatg tgctgcataa tcacggctag catcctccta 6660 tggtacgcac aaatacagcc acactggata gcagcttcaa taatactgga gttttttctc 6720 atagttttgc ttattccaga acctgaaaaa cagagaacac cccaagacaa ccaactgacc 6780 tacgttgtca tagccatcct cacagtggtg gccgcaacca tggcaaacga gatgggtttc 6840 ctagaaaaaa cgaagaaga tctcggattg ggaagcattg caacccagca acccgagagc 6900 aacatcctgg acatagatct acgtcctgca tcagcatgga cgctgtatgc cgtggccaca 6960 acatttgtta caccaatgtt gagacatagc attgaaaatt cctcagtgaa tgtgtcccta 7020 acagctatag ccaaccaagc cacagtgtta atgggtctcg ggaaaggatg gccattgtca 7080 aagatggaca tcggagttcc ccttctcgcc attggatgct actcacaagt caacccata 7140 actctcacag cagctctttt cttattgta gcacattatg ccatcatagg gccaggactc 7200 caagcaaag caccagaa agctcagaaa agagcagcgg cgggcatcat gaaaaaccca 7260 actgtcgatg gataacagt gattgaccta gatccaatac cttatgatcc aaagtttgaa 7320 aagcagttgg gatagtaat gctcctagtc ctctgcgtga ctcaagtatt gatgatgagg 7380 actacatggg ctctgtgtga ggctttaacc ttagctaccg ggcccactc cacattgtgg 7440 gaaggaaatc cagggaggtt ttggacact accattgcgg tgtcaatggc tacattttt 7500 agagggagtt acttggccgg agctggactt ctctttcta ttatgagaa cacaccac 7560 acaagaaggg gaactggcaa cataggagag acgcttggag agaatggaa aagccgattg 7620 aacgcattgg gaaaaagtga attccagatc tacaagaaa gtggaatcca ggaagtggat 7680 agaaccttag caaagaagg cattaaaaga ggagaaacgg accacacgc tgtgtcgcga 7740 ggctcagcaa aactgagatg gttcgttgag agaaacatgg tcacaccaga agggaagta 7800 gtggacctcg gttgtggcag aggaggctgg tcatactatt gtggaggact aagaatgta 7860 agagaagtca aaggcctaac aaaaggagga ccaggacacg aagaacccat ccccatgtca 7920 acatagggt ggaatctagt gcgtcttcaa agtggagttg acgttttctt catcccgcca 7980 gaaaagtgtg acacattatt gtgtgacata ggggagtcat caccaatcc cacagtggaa 8040 gcaggacgaa cactcagagt ccttaactta gtagaaaatt ggttgacaa cacactca 8100 tttgcataa aggttctcaa cccatatatg ccctcagtca tagaaaaat ggaagcacta 8160 haaggaaat atggagc cttagtgagg aatccactct cacgaactc cacacatgag 8220 atgtactggg tatccaatgc ttccgggaac atagtgtcat cagtgaacat gatttcagg 8280 atgttgatca acagatttac aatgagatac aagaaagcca cttacgagcc ggatgttgac 8340 ctcggaagcg gaacccgtaa catcgggatt gaaagtgaga taccaaacct agatataatt 8400 gggaaaagaa tagaaaaaat aaagcaagag catgaaacat catggcacta tgaccaagac 8460 cacccataca aaacgtgggc ataccatggt agctatgaaa caaaacagac tggatcagca 8520 tcatccatgg tcaacggagt ggtcaggctg ctgacaaaac cttgggacgt cgtccccatg 8580 gtgacacaga tggcaatgac agacacgact ccatttggac aacagcgcgt ttttaaagag 8640 aaagtggaca cgagaaccca agaaccgaaa gaaggcacga agaaactaat gaaaataaca 8700 gcagagtggc tttggaaaga attagggaag aaaaagacac ccaggatgtg caccagagaa 8760 gaattcacaa gaaaggtgag aagcaatgca gccttggggg ccgtattcac tgatgagaac 8820 aagtggaagt cggcacgtga ggctgttgaa gatagtaggt tttgggagct ggttgacaag 8880 gaaaggaatc tccatcttga aggaaagtgt gaaacatgtg tgtacaacat gatgggaaaa 8940 agagagaaga agctagggga attcggcaag gcaaaaggca gcagagccat atggtacatg 9000 tggcttggag cacgcttctt agagtttga gccctaggat tctttaatga agatcactgg 9060 ttctccagag agaactccct gagtggagtg gaaggagaag ggctgcacaa gctaggttac 9120 attctaagag acgtgagcaa gaagaggga ggagcaatgt atgccgatga caccgcagga 9180 tgggatacaa gatcacacact agaagaccta aaaaatgaag aaatggtaac aaaccacatg 9240 gaaggagaac acaagaact agccgaggcc atttcaac taacgtacca aaacaggtg 9300 gtgcgtgtgc aaagaccaac accaagaggc acagtaatgg acatcatatc gagagagac 9360 haaagaggta gtggacaagt tggcacctat ggactcaata ctttcaccaa tatggaagcc 9420 caactaatca gagatgga gggagaagga gtctttaaaa gcattcagca cctaacaatc 9480 acagaagaaa tcgctgtgca aaactggtta gcaagagtgg ggcgcgaaag gttatcaaga 9540 atggccatca gtggagatga ttgtgttgtg aaacctttag atgacaggtt cgcaagcgct 9600 tacacagctc taaatgacat gggaagatt aggaagaca tacacaatg ggaaccttca 9660 agaggatgga atgattggac acagtgccc ttctgttcac accattcca tgagttaatc 9720 atgaaagacg gtcgcgtact cgttgttcca tgtagaaacc aagatgaact gattggcaga 9780 gcccgaatct cccaaggagc agggtggtct ttgcgggaga cggcctgttt ggggaagtct 9840 tacgcccaaa tgtggagctt gatgtacttc cacagacgcg acctcaggct ggcggcaaat 9900 gctatttgct cggcagtacc atcacattgg gttccaacaa gtcgaacaac ctggtccata 9960 catgctaaac atgaatggat gacaacggaa gacatgctga cagtctggaa cagggtgtgg 10020 attcaagaaa acccatggat ggaagacaaa actccagtgg aatcatggga ggaaatccca 10080 tacttgggga aaagagaaga ccaatggtgc ggctcattga ttgggttaac aagcagggcc 10140 acctgggcaa agaacatcca agcagcaata aatcaagtta gatcccttat aggcaatgaa 10200 gaatacacag attacatgcc atccatgaaa agattcagaa gagaagagga agaagcagga 10260 gttctgtggt agaaagcaaa actaacatga aacaaggcta gaagtcaggt cggattaagc 10320 catagtacgg aaaaaactat gctacctgtg agccccgtcc aaggacgtta aaagaagtca 10380 ggccatcata aatgccatag cttgagtaaa ctatgcagcc tgtagctcca cctgagaagg 10440 tgtaaaaaat ccgggaggcc acaaaccatg gaagctgtac gcatggcgta gtggactagc 10500 ggttagagga gacccctccc ttacaaatcg cagcaacaat ggggccccaa ggcgagatga 10560 agctgtagtc tcgctgggaag gactagaggt tagaggac ccccccgaaa caaaaaacag 10620 catattgacg ctgggaaaga ccagagatcc tgctgtctcc tcagcatcat tccaggcaca 10680 gaacgccaga aaatggaatg gtgctgttga atcaacaggt tct 10723 <210> 12 <211> 3391 <212> PRT <213> sequence <220> <223> Dengue virus serotype 2, MVS <400> 12 Met Asn Asn Gln Arg Lys Lys Ala Lys Asn Thr Pro Phe Asn Met Leu 1 5 10 15 Lys Arg Glu Arg Asn Arg Val Ser Thr Val Gln Gln Leu Thr Lys Arg 20 25 30 Phe Ser Leu Gly Met Leu Gln Gly Arg Gly Pro Leu Lys Leu Phe Met 35 40 45 Ala Leu Val Ala Phe Leu Arg Phe Leu Thr Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Lys Arg Trp Gly Thr Ile Lys Lys Ser Lys Ala Ile Asn Val 65 70 75 80 Leu Arg Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn 85 90 95 Arg Arg Arg Arg Ser Ala Gly Met Ile Ile Met Leu Ile Pro Thr Val 100 105 110 Met Ala Phe His Leu Thr Thr Arg Asn Gly Glu Pro His Met Ile Val 115 120 125 Ser Arg Gln Glu Lys Gly Lys Ser Leu Leu Phe Lys Thr Glu Val Gly 130 135 140 Val Asn Met Cys Thr Leu Met Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Ile Thr Tyr Glu Cys Pro Leu Leu Arg Gln Asn Glu Pro Glu 165 170 175 Asp Ile Asp Cys Trp Cys Asn Ser Thr Ser Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Thr Thr Met Gly Glu His Arg Arg Glu Lys Arg Ser Val Ala 195 200 205 Leu Val Pro His Val Gly Met Gly Leu Glu Thr Arg Thr Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys His Val Gln Arg Ile Glu Thr Trp 225 230 235 240 Ile Leu Arg His Pro Gly Phe Thr Met Met Ala Ala Ile Leu Ala Tyr 245 250 255 Thr Ile Gly Thr Thr His Phe Gln Arg Ala Leu Ile Phe Ile Leu Leu 260 265 270 Thr Ala Val Thr Pro Ser Met Thr Met Arg Cys Ile Gly Met Ser Asn 275 280 285 Arg Asp Phe Val Glu Gly Val Ser Gly Gly Ser Trp Val Asp Ile Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Phe Glu Leu Ile Lys Thr Glu Ala Lys Gln Pro Ala Thr Leu 325 330 335 Arg Lys Tyr Cys Ile Glu Ala Lys Leu Thr Asn Thr Thr Thr Glu Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Pro Ser Leu Asn Glu Glu Gln Asp Lys 355 360 365 Arg Phe Val Cys Lys His Ser Met Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Gly Ile Val Thr Cys Ala Met Phe Arg 385 390 395 400 Cys Lys Lys Asn Met Glu Gly Lys Val Val Gln Pro Glu Asn Leu Glu 405 410 415 Tyr Thr Ile Val Ile Thr Pro His Ser Gly Glu Glu His Ala Val Gly 420 425 430 Asn Asp Thr Gly Lys His Gly Lys Glu Ile Lys Ile Thr Pro Gln Ser 435 440 445 Ser Ile Thr Glu Ala Glu Leu Thr Gly Tyr Gly Thr Val Thr Met Glu 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Gln 465 470 475 480 Met Glu Asn Lys Ala Trp Leu Val His Arg Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Leu Pro Gly Ala Asp Thr Gln Gly Ser Asn Trp Ile 500 505 510 Gln Lys Glu Thr Leu Val Thr Phe Lys Asn Pro His Ala Lys Lys Gln 515 520 525 Asp Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Met Ser Ser Gly Asn Leu Leu Phe Thr 545 550 555 560 Gly His Leu Lys Cys Arg Leu Arg Met Asp Lys Leu Gln Leu Lys Gly 565 570 575 Met Ser Tyr Ser Met Cys Thr Gly Lys Phe Lys Val Val Lys Glu Ile 580 585 590 Ala Glu Thr Gln His Gly Thr Ile Val Ile Arg Val Gln Tyr Glu Gly 595 600 605 Asp Gly Ser Pro Cys Lys Ile Pro Phe Glu Ile Met Asp Leu Glu Lys 610 615 620 Arg His Val Leu Gly Arg Leu Ile Thr Val Asn Pro Ile Val Thr Glu 625 630 635 640 Lys Asp Ser Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Asp Ser 645 650 655 Tyr Ile Ile Ile Gly Val Glu Pro Gly Gln Leu Lys Leu Asn Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Gln Met Phe Glu Thr Thr Met Arg Gly 675 680 685 Ala Lys Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Leu Gly Gly Val Phe Thr Ser Ile Gly Lys Ala Leu His Gln Val Phe 705 710 715 720 Gly Ala Ile Tyr Gly Ala Ala Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Leu Ile Gly Val Ile Ile Thr Trp Ile Gly Met Asn Ser Arg Ser 740 745 750 Thr Ser Leu Ser Val Thr Leu Val Leu Val Gly Ile Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Val Ser Trp Lys 770 775 780 Asn Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Ile Thr Asp Asn Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ser Lys 805 810 815 Leu Ala Ser Ala Ile Gln Lys Ala His Glu Glu Asp Ile Cys Gly Ile 820 825 830 Arg Ser Val Thr Arg Leu Glu Asn Leu Met Trp Lys Gln Ile Thr Pro 835 840 845 Glu Leu Asn His Ile Leu Ser Glu Asn Glu Val Lys Leu Thr Ile Met 850 855 860 Thr Gly Asp Ile Lys Gly Ile Met Gln Ala Gly Lys Arg Ser Leu Arg 865 870 875 880 Pro Gln Pro Thr Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala 885 890 895 Lys Met Leu Ser Thr Glu Ser His Asn Gln Thr Phe Leu Ile Asp Gly 900 905 910 Pro Glu Thr Ala Glu Cys Pro Asn Thr Asn Arg Ala Trp Asn Ser Leu 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Lys Glu Lys Gln Asp Val Phe Cys Asp Ser Lys Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Asn Arg Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Ala Leu Asn Asp Thr Trp Lys Ile Glu Lys Ala Ser Phe 980 985 990 Ile Glu Val Lys Asn Cys His Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Asn Leu Ala 1010 1015 1020 Gly Pro Val Ser Gln His Asn Tyr Arg Pro Gly Tyr His Thr Gln 1025 1030 1035 Ile Thr Gly Pro Trp His Leu Gly Lys Leu Glu Met Asp Phe Asp 1040 1045 1050 Phe Cys Asp Gly Thr Thr Val Val Val Thr Glu Asp Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Ile 1070 1075 1080 Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr 1085 1090 1095 Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala Gly 1115 1120 1125 His Gly Gln Val Asp Asn Phe Ser Leu Gly Val Leu Gly Met Ala 1130 1135 1140 Leu Phe Leu Glu Glu Met Leu Arg Thr Arg Val Gly Thr Lys His 1145 1150 1155 Ala Ile Leu Leu Val Ala Val Ser Phe Val Thr Leu Ile Thr Gly 1160 1165 1170 Asn Met Ser Phe Arg Asp Leu Gly Arg Val Met Val Met Val Gly 1175 1180 1185 Ala Thr Met Thr Asp Asp Ile Gly Met Gly Val Thr Tyr Leu Ala 1190 1195 1200 Leu Leu Ala Ala Phe Lys Val Arg Pro Thr Phe Ala Ala Gly Leu 1205 1210 1215 Leu Leu Arg Lys Leu Thr Ser Lys Glu Leu Met Met Thr Thr Ile 1220 1225 1230 Gly Ile Val Leu Leu Ser Gln Ser Thr Ile Pro Glu Thr Ile Leu 1235 1240 1245 Glu Leu Thr Asp Ala Leu Ala Leu Gly Met Met Val Leu Lys Met 1250 1255 1260 Val Arg Asn Met Glu Lys Tyr Gln Leu Ala Val Thr Ile Met Ala 1265 1270 1275 Ile Leu Cys Val Pro Asn Ala Val Ile Leu Gln Asn Ala Trp Lys 1280 1285 1290 Val Ser Cys Thr Ile Leu Ala Val Val Ser Val Ser Pro Leu Phe 1295 1300 1305 Leu Thr Ser Ser Gln Gln Lys Thr Asp Trp Ile Pro Leu Ala Leu 1310 1315 1320 Thr Ile Lys Gly Leu Asn Pro Thr Ala Ile Phe Leu Thr Thr Leu 1325 1330 1335 Ser Arg Thr Ser Lys Lys Arg Ser Trp Pro Leu Asn Glu Ala Ile 1340 1345 1350 Met Ala Val Gly Met Val Ser Ile Leu Ala Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Ile Pro Met Thr Gly Pro Leu Val Ala Gly Gly Leu Leu 1370 1375 1380 Thr Val Cys Tyr Val Leu Thr Gly Arg Ser Ala Asp Leu Glu Leu 1385 1390 1395 Glu Arg Ala Ala Asp Val Lys Trp Glu Asp Gln Ala Glu Ile Ser 1400 1405 1410 Gly Ser Ser Pro Ile Leu Ser Ile Thr Ile Ser Glu Asp Gly Ser 1415 1420 1425 Met Ser Ile Lys Asn Glu Glu Glu Glu Gln Thr Leu Thr Ile Leu 1430 1435 1440 Ile Arg Thr Gly Leu Leu Val Ile Ser Gly Leu Phe Pro Val Ser 1445 1450 1455 Ile Pro Ile Thr Ala Ala Ala Trp Tyr Leu Trp Glu Val Lys Lys 1460 1465 1470 Gln Arg Ala Gly Val Leu Trp Asp Val Pro Ser Pro Pro Pro Met 1475 1480 1485 Gly Lys Ala Glu Leu Glu Asp Gly Ala Tyr Arg Ile Lys Gln Lys 1490 1495 1500 Gly Ile Leu Gly Tyr Ser Gln Ile Gly Ala Gly Val Tyr Lys Glu 1505 1510 1515 Gly Thr Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met His Lys Gly Lys Arg Ile Glu Pro Ser Trp Ala Asp Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Lys Leu Glu Gly Glu 1550 1555 1560 Trp Lys Glu Gly Glu Glu Val Gln Val Leu Ala Leu Glu Pro Gly 1565 1570 1575 Lys Asn Pro Arg Ala Val Gln Thr Lys Pro Gly Leu Phe Lys Thr 1580 1585 1590 Asn Ala Gly Thr Ile Gly Ala Val Ser Leu Asp Phe Ser Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Ile Asp Lys Lys Gly Lys Val Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Arg Ser Gly Ala Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Thr Glu Lys Ser Ile Glu Asp Asn Pro Glu Ile 1640 1645 1650 Glu Asp Asp Ile Phe Arg Lys Arg Arg Leu Thr Ile Met Asp Leu 1655 1660 1665 His Pro Gly Ala Gly Lys Thr Lys Arg Tyr Leu Pro Ala Ile Val 1670 1675 1680 Arg Glu Ala Ile Lys Arg Gly Leu Arg Thr Leu Ile Leu Ala Pro 1685 1690 1695 Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu 1700 1705 1710 Pro Ile Arg Tyr Gln Thr Pro Ala Ile Arg Ala Val His Thr Gly 1715 1720 1725 Arg Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg 1730 1735 1740 Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met 1745 1750 1755 Asp Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly 1760 1765 1770 Tyr Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Gly Ile Phe 1775 1780 1785 Met Thr Ala Thr Pro Pro Gly Ser Arg Asp Pro Phe Pro Gln Ser 1790 1795 1800 Asn Ala Pro Ile Ile Asp Glu Glu Arg Glu Ile Pro Glu Arg Ser 1805 1810 1815 Trp Asn Ser Gly His Glu Trp Val Thr Asp Phe Lys Gly Lys Thr 1820 1825 1830 Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Ala 1835 1840 1845 Cys Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys 1850 1855 1860 Thr Phe Asp Ser Glu Tyr Val Lys Thr Arg Thr Asn Asp Trp Asp 1865 1870 1875 Phe Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys 1880 1885 1890 Ala Glu Arg Val Ile Asp Pro Arg Arg Cys Met Lys Pro Val Ile 1895 1900 1905 Leu Thr Asp Gly Glu Glu Arg Val Ile Leu Ala Gly Pro Met Pro 1910 1915 1920 Val Thr His Ser Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg 1925 1930 1935 Asn Pro Lys Asn Glu Asn Asp Gln Tyr Ile Tyr Met Gly Glu Pro 1940 1945 1950 Leu Glu Asn Asp Glu Asp Cys Ala His Trp Lys Glu Ala Lys Met 1955 1960 1965 Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ser Met 1970 1975 1980 Phe Glu Pro Glu Arg Glu Lys Val Asp Ala Ile Asp Gly Glu Tyr 1985 1990 1995 Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Asp Leu Met Arg 2000 2005 2010 Arg Gly Asp Leu Pro Val Trp Leu Ala Tyr Arg Val Ala Ala Glu 2015 2020 2025 Gly Ile Asn Tyr Ala Asp Arg Arg Trp Cys Phe Asp Gly Val Lys 2030 2035 2040 Asn Asn Gln Ile Leu Glu Glu Asn Val Glu Val Glu Ile Trp Thr 2045 2050 2055 Lys Glu Gly Glu Arg Lys Lys Leu Lys Pro Arg Trp Leu Asp Ala 2060 2065 2070 Arg Ile Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Glu Phe 2075 2080 2085 Ala Ala Gly Arg Lys Ser Leu Thr Leu Asn Leu Ile Thr Glu Met 2090 2095 2100 Gly Arg Leu Pro Thr Phe Met Thr Gln Lys Ala Arg Asp Ala Leu 2105 2110 2115 Asp Asn Leu Ala Val Leu His Thr Ala Glu Ala Gly Gly Arg Ala 2120 2125 2130 Tyr Asn His Ala Leu Ser Glu Leu Pro Glu Thr Leu Glu Thr Leu 2135 2140 2145 Leu Leu Leu Thr Leu Leu Ala Thr Val Thr Gly Gly Ile Phe Leu 2150 2155 2160 Phe Leu Met Ser Ala Arg Gly Ile Gly Lys Met Thr Leu Gly Met 2165 2170 2175 Cys Cys Ile Ile Thr Ala Ser Ile Leu Leu Trp Tyr Ala Gln Ile 2180 2185 2190 Gln Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu 2195 2200 2205 Ile Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln 2210 2215 2220 Asp Asn Gln Leu Thr Tyr Val Val Ile Ala Ile Leu Thr Val Val 2225 2230 2235 Ala Ala Thr Met Ala Asn Glu Met Gly Phe Leu Glu Lys Thr Lys 2240 2245 2250 Lys Asp Leu Gly Leu Gly Ser Ile Ala Thr Gln Gln Pro Glu Ser 2255 2260 2265 Asn Ile Leu Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu 2270 2275 2280 Tyr Ala Val Ala Thr Thr Phe Val Thr Pro Met Leu Arg His Ser 2285 2290 2295 Ile Glu Asn Ser Ser Val Asn Val Ser Leu Thr Ala Ile Ala Asn 2300 2305 2310 Gln Ala Thr Val Leu Met Gly Leu Gly Lys Gly Trp Pro Leu Ser 2315 2320 2325 Lys Met Asp Ile Gly Val Pro Leu Leu Ala Ile Gly Cys Tyr Ser 2330 2335 2340 Gln Val Asn Pro Ile Thr Leu Thr Ala Ala Leu Phe Leu Leu Val 2345 2350 2355 Ala His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr 2360 2365 2370 Arg Glu Ala Gln Lys Arg Ala Ala Ala Gly Ile Met Lys Asn Pro 2375 2380 2385 Thr Val Asp Gly Ile Thr Val Ile Asp Leu Asp Pro Ile Pro Tyr 2390 2395 2400 Asp Pro Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val 2405 2410 2415 Leu Cys Val Thr Gln Val Leu Met Met Arg Thr Thr Trp Ala Leu 2420 2425 2430 Cys Glu Ala Leu Thr Leu Ala Thr Gly Pro Ile Ser Thr Leu Trp 2435 2440 2445 Glu Gly Asn Pro Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser 2450 2455 2460 Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu 2465 2470 2475 Leu Phe Ser Ile Met Lys Asn Thr Thr Asn Thr Arg Arg Gly Thr 2480 2485 2490 Gly Asn Ile Gly Glu Thr Leu Gly Glu Lys Trp Lys Ser Arg Leu 2495 2500 2505 Asn Ala Leu Gly Lys Ser Glu Phe Gln Ile Tyr Lys Lys Ser Gly 2510 2515 2520 Ile Gln Glu Val Asp Arg Thr Leu Ala Lys Glu Gly Ile Lys Arg 2525 2530 2535 Gly Glu Thr Asp His His Ala Val Ser Arg Gly Ser Ala Lys Leu 2540 2545 2550 Arg Trp Phe Val Glu Arg Asn Met Val Thr Pro Glu Gly Lys Val 2555 2560 2565 Val Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Gly 2570 2575 2580 Gly Leu Lys Asn Val Arg Glu Val Lys Gly Leu Thr Lys Gly Gly 2585 2590 2595 Pro Gly His Glu Glu Pro Ile Pro Met Ser Thr Tyr Gly Trp Asn 2600 2605 2610 Leu Val Arg Leu Gln Ser Gly Val Asp Val Phe Phe Ile Pro Pro 2615 2620 2625 Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro 2630 2635 2640 Asn Pro Thr Val Glu Ala Gly Arg Thr Leu Arg Val Leu Asn Leu 2645 2650 2655 Val Glu Asn Trp Leu Asn Asn Asn Thr Gln Phe Cys Ile Lys Val 2660 2665 2670 Leu Asn Pro Tyr Met Pro Ser Val Ile Glu Lys Met Glu Ala Leu 2675 2680 2685 Gln Arg Lys Tyr Gly Gly Ala Leu Val Arg Asn Pro Leu Ser Arg 2690 2695 2700 Asn Ser Thr His Glu Met Tyr Trp Val Ser Asn Ala Ser Gly Asn 2705 2710 2715 Ile Val Ser Ser Val Asn Met Ile Ser Arg Met Leu Ile Asn Arg 2720 2725 2730 Phe Thr Met Arg Tyr Lys Lys Ala Thr Tyr Glu Pro Asp Val Asp 2735 2740 2745 Leu Gly Ser Gly Thr Arg Asn Ile Gly Ile Glu Ser Glu Ile Pro 2750 2755 2760 Asn Leu Asp Ile Ile Gly Lys Arg Ile Glu Lys Ile Lys Gln Glu 2765 2770 2775 His Glu Thr Ser Trp His Tyr Asp Gln Asp His Pro Tyr Lys Thr 2780 2785 2790 Trp Ala Tyr His Gly Ser Tyr Glu Thr Lys Gln Thr Gly Ser Ala 2795 2800 2805 Ser Ser Met Val Asn Gly Val Val Arg Leu Leu Thr Lys Pro Trp 2810 2815 2820 Asp Val Val Pro Met Val Thr Gln Met Ala Met Thr Asp Thr Thr 2825 2830 2835 Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg 2840 2845 2850 Thr Gln Glu Pro Lys Glu Gly Thr Lys Lys Leu Met Lys Ile Thr 2855 2860 2865 Ala Glu Trp Leu Trp Lys Glu Leu Gly Lys Lys Lys Thr Pro Arg 2870 2875 2880 Met Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn Ala 2885 2890 2895 Ala Leu Gly Ala Val Phe Thr Asp Glu Asn Lys Trp Lys Ser Ala 2900 2905 2910 Arg Glu Ala Val Glu Asp Ser Arg Phe Trp Glu Leu Val Asp Lys 2915 2920 2925 Glu Arg Asn Leu His Leu Glu Gly Lys Cys Glu Thr Cys Val Tyr 2930 2935 2940 Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys 2945 2950 2955 Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg 2960 2965 2970 Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp 2975 2980 2985 Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly Leu 2990 2995 3000 His Lys Leu Gly Tyr Ile Leu Arg Asp Val Ser Lys Lys Glu Gly 3005 3010 3015 Gly Ala Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile 3020 3025 3030 Thr Leu Glu Asp Leu Lys Asn Glu Glu Met Val Thr Asn His Met 3035 3040 3045 Glu Gly Glu His Lys Lys Leu Ala Glu Ala Ile Phe Lys Leu Thr 3050 3055 3060 Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Thr Pro Arg Gly 3065 3070 3075 Thr Val Met Asp Ile Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly 3080 3085 3090 Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala 3095 3100 3105 Gln Leu Ile Arg Gln Met Glu Gly Glu Gly Val Phe Lys Ser Ile 3110 3115 3120 Gln His Leu Thr Ile Thr Glu Glu Ile Ala Val Gln Asn Trp Leu 3125 3130 3135 Ala Arg Val Gly Arg Glu Arg Leu Ser Arg Met Ala Ile Ser Gly 3140 3145 3150 Asp Asp Cys Val Val Lys Pro Leu Asp Asp Arg Phe Ala Ser Ala 3155 3160 3165 Leu Thr Ala Leu Asn Asp Met Gly Lys Ile Arg Lys Asp Ile Gln 3170 3175 3180 Gln Trp Glu Pro Ser Arg Gly Trp Asn Asp Trp Thr Gln Val Pro 3185 3190 3195 Phe Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg 3200 3205 3210 Val Leu Val Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg 3215 3220 3225 Ala Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala 3230 3235 3240 Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe 3245 3250 3255 His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser Ala 3260 3265 3270 Val Pro Ser His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile 3275 3280 3285 His Ala Lys His Glu Trp Met Thr Thr Glu Asp Met Leu Thr Val 3290 3295 3300 Trp Asn Arg Val Trp Ile Gln Glu Asn Pro Trp Met Glu Asp Lys 3305 3310 3315 Thr Pro Val Glu Ser Trp Glu Glu Ile Pro Tyr Leu Gly Lys Arg 3320 3325 3330 Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala 3335 3340 3345 Thr Trp Ala Lys Asn Ile Gln Ala Ala Ile Asn Gln Val Arg Ser 3350 3355 3360 Leu Ile Gly Asn Glu Glu Tyr Thr Asp Tyr Met Pro Ser Met Lys 3365 3370 3375 Arg Phe Arg Arg Glu Glu Glu Glu Ala Gly Val Leu Trp 3380 3385 3390 <210>13 <211>10723 <212>DNA <213>Artificial Sequence <220> <223> Dengue virus serotype 2, WVS <220> <221> misc_feature <222> (6481)..(6481) <223> n is cytosine or guanine <220> <221> misc_feature <222> (7156)..(7156) <223> n is cytosine or thymine <400> 13 agttgttagt ctacgtggac cgacaaagac agattctttg agggagctaa gctcaatgta 60 gttctaacag ttttttaatt agagagcaga tctctgatga ataaccaacg gaaaaaggcg 120 aaaaacacgc ctttcaatat gctgaaacgc gagagaaacc gcgtgtcgac tgtgcaacag 180 ctgacaaaga gattctcact tggaatgctg cagggacgag gaccattaaa actgttcatg 240 gccctggtgg cgttccttcg tttcctaaca atcccaccaa cagcagggat attgaagaga 300 tggggaacaa ttaaaaaatc aaaagctatt aatgttttga gagggttcag gaaagagatt 360 ggaaggatgc tgaacatctt gaataggaga cgcagatctg caggcatgat cattatgctg 420 attccaacag tgatggcgtt ccatttaacc acacgtaacg gagaaccaca catgatcgtc 480 agcagacaag agaaagggaa aagtcttctg tttaaaacag aggttggcgt gaacatgtgt 540 accctcatgg ccatggacct tggtgaattg tgtgagaca caatcacgta cgagtgtccc 600 cttctcaggc agaatgagcc agagacata gactgttggt gcaacctac gtccacgtgg 660 gtaacttatg ggacgtgtac caccatggga gaacatagaa gagaaaaag atcagtggca 720 ctcgttccac atgtgggaat gggactggag acacgactg aaacatggat gtcatcagaa 780 ggggcctgga aacatgtcca gagaattgaa acttggatct tgagacatcc aggctcacc 840 atgatggcag caatcctggc atacaccata ggaacgacac atttccaag agccctgatc 900 ttcatcttac tgacagctgt cactccttca atgacaatgc gttgcatagg atgtcaaat 960 agagactttg tggaggggt tcaggagga agctggttg acatagtctt agaacatgga 1020 agctgtgtga cgacgatgc aaaaaaaaaaaacattgg attttgaact gataaaaaaca 1080 gagccaaac agcctgccac cctaggaag tactgtatag agggcaagct aaccaaca 1140 acaacagaat ctcgctgccc aacacaaggg gaacccagcc taaatgaga gcaggacaaa 1200 aggttcgtct gcaacactc catggtagac agaggatggg gaatggatg tggactattt 1260 ggaagggag gcattgtgac ctgtgctatg ttcagatgca aaaagaacat ggaaggaaaa 1320 gttgtgcaac cagaaaactt ggaatacacc attgtgataa cacctcactc agggaagaag 1380 1440 tccatcacag aagcagaatt gacaggttat ggcactgtca caatggagtg ctctccaaga 1500 acgggcctcg acttcaatga gatggtgttg ctgcagatgg aaaataagc ttggctggtg 1560 cacaggcaat ggttcctaga cctgccgtta ccatggttgc ccggagcgga cacaaggg 1620 tcaaattgga tacagaaaga gacattggtc actttcaaaa atccccatgc gaaaacag 1680 gatgttgttg ttttaggatc ccaagaaggg gccatgcaca cagcacttac aggggccaca 1740 gaaatccaaa tgtcatcagg aaacttactc ttcacaggac atctcaagtg caggctgaga 1800 1860 gtgaaggaaa tagcagaaac acaacatgga acaatagtta tcagagtgca atatgaaggg 1920 gacggctctc catgcaagat cccttttgag ataatggatt tggaaaaaag acatgtctta 1980 ggtcgcctga ttacagtcaa cccaattgtg acagaaaaag atagcccagt cacatagaa 2040 gcagaacctc cattggag cagctacatc atcataggag tagagccggg acaactgaag 2100 ctcaactggt ttaagaagg aagttctatc ggccaatgt ttgagacaac atgaggggg 2160 gcgaagagaa tggccattt aggtgacaca gctgggattttggttctt gggaggagtg 2220 tttacatcta taggaagggc tctccaccaa gtctttggag caatctatgg agctgccttc 2280 agtggggttt catggactt gaaaatccctc ataggagtca ttatcacatg gatggaatg 2340 aattcacgca gcacctcact gtctgtgaca ctagtattgg tgggaattgt jagactgtat 2400 ttgggagtca tggtgcaggc cgatagtggt tgcgttgtga gctggaaaaa caagaactg 2460 aaatgtggca gtgggattt catcacagac aacgtgcaca catggacaga acatacaag 2520 ttccaaccag aatccccttc aaaactagct tcagctatcc agaagccca tgaagaggac 2580 atttgtggaa tccgctcagt aacagactg gagaatctga tgtggaaca aaacacca 2640 gattgaatc acattctatc agaaaatgag gtgaagttaa ctattatgac aggagacatc 2700 aaaggaatca tgcaggcagg aaacgatct ctgcggccctc agcccactga gctgaagtat 2760 tcatggaaaa catggggcaa agcaaaatg ctctctacag agtctcataa ccagaccttt 2820 ctcattgatg gccccgaac agcagaatgc cccacaca atagagcttg gattcgttg 2880 gaagttgaag actatggctt tggagtattc accaccaata tattggctaaa attgaaagaa 2940 aaaacaggatg tattctgcga ctcaaactc atgtcagcgg ccaaaaga caacagagcc 3000 gtccatgccg attgggtta ttggatagaa agtgcactca atgacacatg gaagatagag 3060 aaagcctctt tcattgaagt taaaaactgc cactggccaa atcacacac cctctggagc 3120 aatggagtgc tagaagtga gatgatatt ccaagaatc tcgctggacc agtgtctca 3180 cacaactata gaccaggcta ccatacacaa atacaggac catggcatct aggtaagctt 3240 gagatgact tgatttctg tgatgacaca acagtgtag tgactgagga ctgcggaat 3300 agaggaccct ctttgagac aaccactgcc tctggaaac tcatacaga atggtgctgc 3360 cgatcttgca cattaccacc gctaagatac agaggtgagg atggttgctg gtacgggatg 3420 gaatcagac cattgaagga gaagaagag aatttggtca actccttggt cacagctgga 3480 3540 atgcttagga cccgagtagg aacgaaacat gcaatactac tagttgcagt ttcttttgtg 3600 acattgatca cagggaacat gtcctttaga gacctgggaa gagtgatggt tatggtaggc 3660 gccactatga cggatgacat aggtatgggc gtgacttatc ttgccctact agcagccttc 3720 aaagtcagac caacttttgc agctggacta ctcttgagaa agctgacctc caaggaattg 3780 atgatgacta ctataggaat tgtactcctc tcccagagca ccataccaga gaccattctt 3840 gagttgactg atgcgttagc cttaggcatg atggtcctca aaatggtgag aaatatggaa 3900 aagtatcaat tggcagtgac tatcatggct atcttgtgcg tcccaaacgc agtgatatta 3960 caaaacgcat ggaagtgag ttgcacaata ttggcagtgg tgtccgtttc cccactgttc 4020 ttaacatcct cacagcaaaa aacagattgg ataccattag cattgacgat caaaggtctc 4080 aatccaacag ctattttct aacaaccctc tcaagaacca gcaagaaaag gagctggcca 4140 ttaatgagg ctatcatggc agtcgggatg gtgagcattt tagccagttc tctcctaaaa aatgatattc ccatgacagg accattagtg gctggagggc tcctcactgt gtgctacgtg 4260 ctcactggac gatcggccga tttggaactg gagagagcag ccgatgtcaa atgggaagac 4380. 4380. 4380. 4380. 4380. 4380. 4380. 4380. 4380 atgtcgataa aaaatgaaga ggaagaacaa acactgacca tactcattag aacaggattg ctggtgatct caggactttt tcctgtatca attack cggcagcagc atggtacctg tgggaagtga agaaacaacg ggccggagta ttgtgggatg ttccttcacc cccacccatg 4560. ggaaaggctg aactggaaga tggagcctat agaattaagc aaaaagggat tcttggatat tcccagatcg gagccggagt ttacaaagaa ggaacattcc atacaatgtg gcatgtcaca cgtggcgctg ttctaatgca taaaggaaag aggattgaac catcatgggc ggacgtcaag aaagacctaa tatcatatgg aggaggctgg aagttagaag gagaatgga ggaggaga gagtccagg tattggcact gagcctgga aaaaatcca gagccgtcca aacgaaacct ggtcttttca aaaccaacgc cggaacaata ggtgctgtat ctctggactt ttctcctgga 4920 acgtcaggat ctccaattat cgacaaaaaa ggaaaagttg tgggtcttta tggtaatggt 4980 gttgttacaa ggagtggagc atatgtgagt gctatagccc agactgaaaa aagcattgaa 5040 gacaacccag agatcgaaga tgacattttc cgaaagagaa gactgaccat catggacctc 5100 cacccaggag cgggaaagac gaagagatac cttccggcca tagtcagaga agctataaaaa 5160 cggggtttga gaacattaat cttggccccc actagagttg tggcagctga aatggaggaa 5220 gcccttagag gacttccaat aagataccag accccagcca tcagagctgt gcacaccggg 5280 cgggagattg tggacctaat gtgtcatgcc acatttacca tgaggctgct atcaccagtt 5340 agagtgccaa actacaacct gattatcatg gacgaagccc atttcacaga cccagcaagt 5400 atagcagcta gaggatacat ctcaactcga gtggagatgg gtgaggcagc tgggattttt 5460 atgacagcca ctccccccggg aagcagagac ccatttcctc agagcaatgc accaatcata 5520 gatgaagaaa gagaaatccc tgaacgctcg tggaattccg gacatgaatg ggtcacggat 5580 tttaaaggga agactgttg gttcgttcca agtataaaag caggaatga tatagcagct 5640 tgcctgagga aaatggaaa gaagtgata caaccagta ggaagacctt tgattctgag 5700 tatgtcaaga ctagaaccaa tgattgggac ttcgtggtta caactgacat ttcagaatg 5760 ggtgccaatt tcaggctga gagggttata gacccagac gctgcatgaa accagtcata 5820 ctaacagatg gtgaagagcg gtgattctg gcaggaccta tgccagtgac ccacttagt 5880 gcagcacaaa gagagggaaaaggaaaatcaaaaaaaaaaagaaa ccagtacata 5940 tacatggggg aacctctgga aaatgatgaa gactgtgcac actggaaga agctaaaatg 6000 ctcctagata acatcacac gccagaagga atcattccta gcatgttcga accagagcgt 6060 gaaaaggtgg atgccattga tggcgaatac cgcttgagag gagaagcaag gaaaaccttt 6120 gtagacttaa tgagagagg agacctacca gtctggttgg cctacagagt ggcagctgaa 6180 ggcatcaac acgcagacag aaggtggtgt ttgatggag tcaacaa ccaaatccta 6240 gagaaaacg tggattga atctggaca aaagaagggg aaaggaaa attgaaccc 6300 agatggttgg atgctaggat ctattctgac ccactggcgc taaaagaatt taggaattt 6360 gcagccggaa gaaagtctct gaccctgaac ctaatcacag aaatgggtag gctcccaacc 6420 ttcatgactc agaagcaag agacgcactg gacaacttag cagtgctgca cacggctgag 6480 ncaggtggaa gggcgtacaa ccatgctctc agtgaactgc cggagaccct ggagacattg 6540 cttttactga cacttctggc tacagtcacg ggagggatct ttttattctt gatgagcgca 6600 aggggcatag ggaagatgac cctgggaatg tgctgcataa tcacggctag catcctccta 6660 tggtacgcac aaatacagcc acactggata gcagcttcaa taatactgga gttttttctc 6720 atagttttgc ttattccaga acctgaaaaa cagagaacac cccaagacaa ccaactgacc 6780 tacgttgtca tagccatcct cacagtggtg gccgcaacca tggcaaacga gatgggtttc 6840 ctagaaaaaa cgaaaaaga tctcggattg ggaagcattg caacccagca acccgagagc 6900 aacatcctgg acatagatct acgtcctgca tcagcatgga cgctgtatgc cgtggccaca 6960 acatttgtta caccaatgtt gagacatagc attgaaaatt cctcagtgaa tgtgtcccta 7020 acagctatag ccaaccaagc cacagtgtta atgggtctcg ggaaaggatg gccattgtca 7080 aagatggaca tcggagttcc ccttctcgcc attggatgct actcacaagt caacccata 7140 actctcacag cagctnttt cttattgta gcacattatg ccatcatagg gccaggactc 7200 caagcaaag caccagaa agctcagaaa agagcagcgg cgggcatcat gaaaaaccca 7260 actgtcgatg gataacagt gattgaccta gatccaatac cttatgatcc aaagtttgaa 7320 aagcagttgg gatagtaat gctcctagtc ctctgcgtga ctcaagtatt gatgatgagg 7380 actacatggg ctctgtgtga ggctttaacc ttagctaccg ggcccactc cacattgtgg 7440 gaaggaaatc cagggaggtt ttggacact accattgcgg tgtcaatggc tacattttt 7500 agagggagtt acttggccgg agctggactt ctctttcta ttatgagaa cacaccac 7560 acaagaaggg gaactggcaa cataggagag acgcttggag agaatggaa aagccgattg 7620 aacgcattgg gaaaaagtga attccagatc tacaagaaa gtggaatcca ggaagtggat 7680 agaaccttag caaagaagg cattaaaaga ggagaaacgg accacacgc tgtgtcgcga 7740 ggctcagcaa aactgagatg gttcgttgag agaaacatgg tcacaccaga agggaagta 7800 gtggacctcg gttgtggcag aggaggctgg tcatactatt gtggaggact aagaatgta 7860 agagaagtca aaggcctaac aaaaggagga ccaggacacg aagaacccat ccccatgtca 7920 acatagggt ggaatctagt gcgtcttcaa agtggagttg acgttttctt catcccgcca 7980 gaaaagtgtg acacattatt gtgtgacata ggggagtcat caccaatcc cacagtggaa 8040 gcaggacgaa cactcagagt ccttaactta gtagaaaatt ggttgacaa cacactca 8100 tttgcataa aggttctcaa cccatatatg ccctcagtca tagaaaaat ggaagcacta 8160 haaggaaat atggagc cttagtgagg aatccactct cacgaactc cacacatgag 8220 atgtactggg tatccaatgc ttccgggaac atagtgtcat cagtgaacat gatttcagg 8280 atgttgatca acgatttac aatgagatac aagaaagcca cttacgagcc ggatgttgac 8340 ctcggaagcg gaacccgtaa catcgggatt gaagtgaga taccaacct agatatatt 8400 gggaaaagaa tagaaaaat aaagcaagg catgaaacat catggcacta tgaccagac 8460 cacccataca aaacgtgggc ataccatggt agctatgaaa caaaacagac tggatcagca 8580. tcatccatgg tcaacggagt ggtcaggctg ctgacaaaac cttgggacgt cgtccccatg gtgacacaga tggcaatgac agacacgact ccatttggac aacagcgcgt ttttaaagag aaagtggaca cgagaaccca agaccgaa aagacga agaaactat gaaataca gcagagtggc tttggaaga attagggaag aaaaagacac ccaggatgtg caccagaga 8820. gccttggggg ccgtattcac tgatgagac aagtggaagt cggcacgtga ggctgttgaa gatagtaggt tttgggagct ggttgacaag gaaaggaatc tccatcttga aggaaagtgt gaaacatgtg tgtacaacat gatgggaaaa aggagaaga agctagggga attcggcaag gcaaaaggca gcagagccat atggtacatg tggcttggag cacgcttctt agagtttga gccctaggat tcttaaatga agatcactgg 9060 ttctccagag agaactccct gagtggagtg gaggagag ggctgcacaa gctaggttac attctaagag acgtgagcaa gaaagaggga ggagcaatgt atgccgatga caccgcagga tgggatacaa gatcacacact agaagaccta aaaaatgaag aaatggtaac aaaccacatg 9240 gaaggagaac acaagaact agccgaggcc atttcaac taacgtacca aaacaggtg 9300 gtgcgtgtgc aaagaccaac accaagaggc acagtaatgg acatcatatc gagagagac 9360 haaagaggta gtggacaagt tggcacctat ggactcaata ctttcaccaa tatggaagcc 9420 caactaatca gagatgga gggagaagga gtctttaaaa gcattcagca cctaacaatc 9480 acagaagaaa tcgctgtgca aactggtta gcaagagtgg ggcgcgaaag gttatcaaga 9540 atggccatca gtggagatga ttgtgttgtg aaacctttag atgacaggtt cgcaagcgct 9600 ttaacagctc taaatgacat gggaagatt aggaagaca tacacaatg ggaaccttca 9660 agaggatgga atgattggac acaagtgccc ttctgttcac accattcca tgagttaatc 9720 atgaaagacg gtcgcgtact cgttgttcca tgtagaaacc aagatgaact gattggcaga 9780 gcccgaatct cccaaggagc agggtggtct ttgcgggaga cggcctgtttt ggggaagtct 9840 tacgcccaaa tgtggagctt gatgtacttc cacagacgcg acctcaggct ggcggcaaat 9900 gctatttgct cggcagtacc atcacattgg gttccaacaa gtcgaacaac ctggtccata 9960 catgctaaac atgaatggat gacaacggaa gacatgctga cagtctggaa cagggtgtgg 10020 attcaagaaa acccatggat ggaagacaaa actccagtgg aatcatggga ggaaatccca 10080 tacttgggga aaagagaaga ccaatggtgc ggctcattga ttgggttaac aagcagggcc 10140 acctgggcaa agaacatcca agcagcaata aatcaagtta gatcccttat aggcaatgaa 10200 gaatacacag attacatgcc atccatgaaa agattcagaa gagaagagga agaagcagga 10260 gttctgtggt agaaagcaaa actaacatga aacaaggcta gaagtcaggt cggattaagc 10320 catagtacgg aaaaaactat gctacctgtg agccccgtcc aaggacgtta aaagaagtca 10380 ggccatcata aatgccatag cttgagtaaa ctatgcagcc tgtagctcca cctgagaagg 10440 tgtaaaaaat ccgggaggcc acaaaccatg gaagctgtac gcatggcgta gtggactagc 10500 ggttagagga gacccctccc ttacaaatcg cagcaacaat gggggcccaa ggcgagatga 10560 agctgtagtc tcgctggaag gactagaggt tagaggagac ccccccgaaa caaaaaacag 10620 catattgacg ctgggaaaga ccagagatcc tgctgtctcc tcagcatcat tccaggcaca 10680 gaacgccaga aaatggaatg gtgctgttga atcaacaggt tct 10723 <210> 14 <211> 3391 <212> PRT <213> Artificial sequence <220> <223> Dengue virus serotype 2, WVS <220> <221> misc_feature <222> (2129)..(2129) <223> Xaa is alanine or proline <220> <221> misc_feature <222> (2354)..(2354) <223> Xaa is leucine or phenylalanine <400> 14 Met Asn Asn Gln Arg Lys Lys Ala Lys Asn Thr Pro Phe Asn Met Leu 1 5 10 15 Lys Arg Glu Arg Asn Arg Val Ser Thr Val Gln Gln Leu Thr Lys Arg 20 25 30 Phe Ser Leu Gly Met Leu Gln Gly Arg Gly Pro Leu Lys Leu Phe Met 35 40 45 Ala Leu Val Ala Phe Leu Arg Phe Leu Thr Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Lys Arg Trp Gly Thr Ile Lys Lys Ser Lys Ala Ile Asn Val 65 70 75 80 Leu Arg Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn 85 90 95 Arg Arg Arg Arg Ser Ala Gly Met Ile Ile Met Leu Ile Pro Thr Val 100 105 110 Met Ala Phe His Leu Thr Thr Arg Asn Gly Glu Pro His Met Ile Val 115 120 125 Ser Arg Gln Glu Lys Gly Lys Ser Leu Leu Phe Lys Thr Glu Val Gly 130 135 140 Val Asn Met Cys Thr Leu Met Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Ile Thr Tyr Glu Cys Pro Leu Leu Arg Gln Asn Glu Pro Glu 165 170 175 Asp Ile Asp Cys Trp Cys Asn Ser Thr Ser Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Thr Thr Met Gly Glu His Arg Arg Glu Lys Arg Ser Val Ala 195 200 205 Leu Val Pro His Val Gly Met Gly Leu Glu Thr Arg Thr Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys His Val Gln Arg Ile Glu Thr Trp 225 230 235 240 Ile Leu Arg His Pro Gly Phe Thr Met Met Ala Ala Ile Leu Ala Tyr 245 250 255 Thr Ile Gly Thr Thr His Phe Gln Arg Ala Leu Ile Phe Ile Leu Leu 260 265 270 Thr Ala Val Thr Pro Ser Met Thr Met Arg Cys Ile Gly Met Ser Asn 275 280 285 Arg Asp Phe Val Glu Gly Val Ser Gly Gly Ser Trp Val Asp Ile Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Phe Glu Leu Ile Lys Thr Glu Ala Lys Gln Pro Ala Thr Leu 325 330 335 Arg Lys Tyr Cys Ile Glu Ala Lys Leu Thr Asn Thr Thr Thr Glu Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Pro Ser Leu Asn Glu Glu Gln Asp Lys 355 360 365 Arg Phe Val Cys Lys His Ser Met Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Gly Ile Val Thr Cys Ala Met Phe Arg 385 390 395 400 Cys Lys Lys Asn Met Glu Gly Lys Val Val Gln Pro Glu Asn Leu Glu 405 410 415 Tyr Thr Ile Val Ile Thr Pro His Ser Gly Glu Glu His Ala Val Gly 420 425 430 Asn Asp Thr Gly Lys His Gly Lys Glu Ile Lys Ile Thr Pro Gln Ser 435 440 445 Ser Ile Thr Glu Ala Glu Leu Thr Gly Tyr Gly Thr Val Thr Met Glu 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Gln 465 470 475 480 Met Glu Asn Lys Ala Trp Leu Val His Arg Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Leu Pro Gly Ala Asp Thr Gln Gly Ser Asn Trp Ile 500 505 510 Gln Lys Glu Thr Leu Val Thr Phe Lys Asn Pro His Ala Lys Lys Gln 515 520 525 Asp Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Met Ser Ser Gly Asn Leu Leu Phe Thr 545 550 555 560 Gly His Leu Lys Cys Arg Leu Arg Met Asp Lys Leu Gln Leu Lys Gly 565 570 575 Met Ser Tyr Ser Met Cys Thr Gly Lys Phe Lys Val Val Lys Glu Ile 580 585 590 Ala Glu Thr Gln His Gly Thr Ile Val Ile Arg Val Gln Tyr Glu Gly 595 600 605 Asp Gly Ser Pro Cys Lys Ile Pro Phe Glu Ile Met Asp Leu Glu Lys 610 615 620 Arg His Val Leu Gly Arg Leu Ile Thr Val Asn Pro Ile Val Thr Glu 625 630 635 640 Lys Asp Ser Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Asp Ser 645 650 655 Tyr Ile Ile Ile Gly Val Glu Pro Gly Gln Leu Lys Leu Asn Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Gln Met Phe Glu Thr Thr Met Arg Gly 675 680 685 Ala Lys Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Leu Gly Gly Val Phe Thr Ser Ile Gly Lys Ala Leu His Gln Val Phe 705 710 715 720 Gly Ala Ile Tyr Gly Ala Ala Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Leu Ile Gly Val Ile Ile Thr Trp Ile Gly Met Asn Ser Arg Ser 740 745 750 Thr Ser Leu Ser Val Thr Leu Val Leu Val Gly Ile Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Val Ser Trp Lys 770 775 780 Asn Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Ile Thr Asp Asn Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ser Lys 805 810 815 Leu Ala Ser Ala Ile Gln Lys Ala His Glu Glu Asp Ile Cys Gly Ile 820 825 830 Arg Ser Val Thr Arg Leu Glu Asn Leu Met Trp Lys Gln Ile Thr Pro 835 840 845 Glu Leu Asn His Ile Leu Ser Glu Asn Glu Val Lys Leu Thr Ile Met 850 855 860 Thr Gly Asp Ile Lys Gly Ile Met Gln Ala Gly Lys Arg Ser Leu Arg 865 870 875 880 Pro Gln Pro Thr Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala 885 890 895 Lys Met Leu Ser Thr Glu Ser His Asn Gln Thr Phe Leu Ile Asp Gly 900 905 910 Pro Glu Thr Ala Glu Cys Pro Asn Thr Asn Arg Ala Trp Asn Ser Leu 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Lys Glu Lys Gln Asp Val Phe Cys Asp Ser Lys Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Asn Arg Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Ala Leu Asn Asp Thr Trp Lys Ile Glu Lys Ala Ser Phe 980 985 990 Ile Glu Val Lys Asn Cys His Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Asn Leu Ala 1010 1015 1020 Gly Pro Val Ser Gln His Asn Tyr Arg Pro Gly Tyr His Thr Gln 1025 1030 1035 Ile Thr Gly Pro Trp His Leu Gly Lys Leu Glu Met Asp Phe Asp 1040 1045 1050 Phe Cys Asp Gly Thr Thr Val Val Val Thr Glu Asp Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Ile 1070 1075 1080 Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr 1085 1090 1095 Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala Gly 1115 1120 1125 His Gly Gln Val Asp Asn Phe Ser Leu Gly Val Leu Gly Met Ala 1130 1135 1140 Leu Phe Leu Glu Glu Met Leu Arg Thr Arg Val Gly Thr Lys His 1145 1150 1155 Ala Ile Leu Leu Val Ala Val Ser Phe Val Thr Leu Ile Thr Gly 1160 1165 1170 Asn Met Ser Phe Arg Asp Leu Gly Arg Val Met Val Met Val Gly 1175 1180 1185 Ala Thr Met Thr Asp Asp Ile Gly Met Gly Val Thr Tyr Leu Ala 1190 1195 1200 Leu Leu Ala Ala Phe Lys Val Arg Pro Thr Phe Ala Ala Gly Leu 1205 1210 1215 Leu Leu Arg Lys Leu Thr Ser Lys Glu Leu Met Met Thr Thr Ile 1220 1225 1230 Gly Ile Val Leu Leu Ser Gln Ser Thr Ile Pro Glu Thr Ile Leu 1235 1240 1245 Glu Leu Thr Asp Ala Leu Ala Leu Gly Met Met Val Leu Lys Met 1250 1255 1260 Val Arg Asn Met Glu Lys Tyr Gln Leu Ala Val Thr Ile Met Ala 1265 1270 1275 Ile Leu Cys Val Pro Asn Ala Val Ile Leu Gln Asn Ala Trp Lys 1280 1285 1290 Val Ser Cys Thr Ile Leu Ala Val Val Ser Val Ser Pro Leu Phe 1295 1300 1305 Leu Thr Ser Ser Gln Gln Lys Thr Asp Trp Ile Pro Leu Ala Leu 1310 1315 1320 Thr Ile Lys Gly Leu Asn Pro Thr Ala Ile Phe Leu Thr Thr Leu 1325 1330 1335 Ser Arg Thr Ser Lys Lys Arg Ser Trp Pro Leu Asn Glu Ala Ile 1340 1345 1350 Met Ala Val Gly Met Val Ser Ile Leu Ala Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Ile Pro Met Thr Gly Pro Leu Val Ala Gly Gly Leu Leu 1370 1375 1380 Thr Val Cys Tyr Val Leu Thr Gly Arg Ser Ala Asp Leu Glu Leu 1385 1390 1395 Glu Arg Ala Ala Asp Val Lys Trp Glu Asp Gln Ala Glu Ile Ser 1400 1405 1410 Gly Ser Ser Pro Ile Leu Ser Ile Thr Ile Ser Glu Asp Gly Ser 1415 1420 1425 Met Ser Ile Lys Asn Glu Glu Glu Glu Gln Thr Leu Thr Ile Leu 1430 1435 1440 Ile Arg Thr Gly Leu Leu Val Ile Ser Gly Leu Phe Pro Val Ser 1445 1450 1455 Ile Pro Ile Thr Ala Ala Ala Trp Tyr Leu Trp Glu Val Lys Lys 1460 1465 1470 Gln Arg Ala Gly Val Leu Trp Asp Val Pro Ser Pro Pro Pro Met 1475 1480 1485 Gly Lys Ala Glu Leu Glu Asp Gly Ala Tyr Arg Ile Lys Gln Lys 1490 1495 1500 Gly Ile Leu Gly Tyr Ser Gln Ile Gly Ala Gly Val Tyr Lys Glu 1505 1510 1515 Gly Thr Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met His Lys Gly Lys Arg Ile Glu Pro Ser Trp Ala Asp Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Lys Leu Glu Gly Glu 1550 1555 1560 Trp Lys Glu Gly Glu Glu Val Gln Val Leu Ala Leu Glu Pro Gly 1565 1570 1575 Lys Asn Pro Arg Ala Val Gln Thr Lys Pro Gly Leu Phe Lys Thr 1580 1585 1590 Asn Ala Gly Thr Ile Gly Ala Val Ser Leu Asp Phe Ser Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Ile Asp Lys Lys Gly Lys Val Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Arg Ser Gly Ala Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Thr Glu Lys Ser Ile Glu Asp Asn Pro Glu Ile 1640 1645 1650 Glu Asp Asp Ile Phe Arg Lys Arg Arg Leu Thr Ile Met Asp Leu 1655 1660 1665 His Pro Gly Ala Gly Lys Thr Lys Arg Tyr Leu Pro Ala Ile Val 1670 1675 1680 Arg Glu Ala Ile Lys Arg Gly Leu Arg Thr Leu Ile Leu Ala Pro 1685 1690 1695 Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu 1700 1705 1710 Pro Ile Arg Tyr Gln Thr Pro Ala Ile Arg Ala Val His Thr Gly 1715 1720 1725 Arg Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg 1730 1735 1740 Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met 1745 1750 1755 Asp Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly 1760 1765 1770 Tyr Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Gly Ile Phe 1775 1780 1785 Met Thr Ala Thr Pro Pro Gly Ser Arg Asp Pro Phe Pro Gln Ser 1790 1795 1800 Asn Ala Pro Ile Ile Asp Glu Glu Arg Glu Ile Pro Glu Arg Ser 1805 1810 1815 Trp Asn Ser Gly His Glu Trp Val Thr Asp Phe Lys Gly Lys Thr 1820 1825 1830 Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Ala 1835 1840 1845 Cys Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys 1850 1855 1860 Thr Phe Asp Ser Glu Tyr Val Lys Thr Arg Thr Asn Asp Trp Asp 1865 1870 1875 Phe Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys 1880 1885 1890 Ala Glu Arg Val Ile Asp Pro Arg Arg Cys Met Lys Pro Val Ile 1895 1900 1905 Leu Thr Asp Gly Glu Glu Arg Val Ile Leu Ala Gly Pro Met Pro 1910 1915 1920 Val Thr His Ser Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg 1925 1930 1935 Asn Pro Lys Asn Glu Asn Asp Gln Tyr Ile Tyr Met Gly Glu Pro 1940 1945 1950 Leu Glu Asn Asp Glu Asp Cys Ala His Trp Lys Glu Ala Lys Met 1955 1960 1965 Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ser Met 1970 1975 1980 Phe Glu Pro Glu Arg Glu Lys Val Asp Ala Ile Asp Gly Glu Tyr 1985 1990 1995 Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Asp Leu Met Arg 2000 2005 2010 Arg Gly Asp Leu Pro Val Trp Leu Ala Tyr Arg Val Ala Ala Glu 2015 2020 2025 Gly Ile Asn Tyr Ala Asp Arg Arg Trp Cys Phe Asp Gly Val Lys 2030 2035 2040 Asn Asn Gln Ile Leu Glu Glu Asn Val Glu Val Glu Ile Trp Thr 2045 2050 2055 Lys Glu Gly Glu Arg Lys Lys Leu Lys Pro Arg Trp Leu Asp Ala 2060 2065 2070 Arg Ile Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Glu Phe 2075 2080 2085 Ala Ala Gly Arg Lys Ser Leu Thr Leu Asn Leu Ile Thr Glu Met 2090 2095 2100 Gly Arg Leu Pro Thr Phe Met Thr Gln Lys Ala Arg Asp Ala Leu 2105 2110 2115 Asp Asn Leu Ala Val Leu His Thr Ala Glu Xaa Gly Gly Arg Ala 2120 2125 2130 Tyr Asn His Ala Leu Ser Glu Leu Pro Glu Thr Leu Glu Thr Leu 2135 2140 2145 Leu Leu Leu Thr Leu Leu Ala Thr Val Thr Gly Gly Ile Phe Leu 2150 2155 2160 Phe Leu Met Ser Ala Arg Gly Ile Gly Lys Met Thr Leu Gly Met 2165 2170 2175 Cys Cys Ile Ile Thr Ala Ser Ile Leu Leu Trp Tyr Ala Gln Ile 2180 2185 2190 Gln Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu 2195 2200 2205 Ile Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln 2210 2215 2220 Asp Asn Gln Leu Thr Tyr Val Val Ile Ala Ile Leu Thr Val Val 2225 2230 2235 Ala Ala Thr Met Ala Asn Glu Met Gly Phe Leu Glu Lys Thr Lys 2240 2245 2250 Lys Asp Leu Gly Leu Gly Ser Ile Ala Thr Gln Gln Pro Glu Ser 2255 2260 2265 Asn Ile Leu Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu 2270 2275 2280 Tyr Ala Val Ala Thr Thr Phe Val Thr Pro Met Leu Arg His Ser 2285 2290 2295 Ile Glu Asn Ser Ser Val Asn Val Ser Leu Thr Ala Ile Ala Asn 2300 2305 2310 Gln Ala Thr Val Leu Met Gly Leu Gly Lys Gly Trp Pro Leu Ser 2315 2320 2325 Lys Met Asp Ile Gly Val Pro Leu Leu Ala Ile Gly Cys Tyr Ser 2330 2335 2340 Gln Val Asn Pro Ile Thr Leu Thr Ala Ala Xaa Phe Leu Leu Val 2345 2350 2355 Ala His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr 2360 2365 2370 Arg Glu Ala Gln Lys Arg Ala Ala Ala Gly Ile Met Lys Asn Pro 2375 2380 2385 Thr Val Asp Gly Ile Thr Val Ile Asp Leu Asp Pro Ile Pro Tyr 2390 2395 2400 Asp Pro Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val 2405 2410 2415 Leu Cys Val Thr Gln Val Leu Met Met Arg Thr Thr Trp Ala Leu 2420 2425 2430 Cys Glu Ala Leu Thr Leu Ala Thr Gly Pro Ile Ser Thr Leu Trp 2435 2440 2445 Glu Gly Asn Pro Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser 2450 2455 2460 Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu 2465 2470 2475 Leu Phe Ser Ile Met Lys Asn Thr Thr Asn Thr Arg Arg Gly Thr 2480 2485 2490 Gly Asn Ile Gly Glu Thr Leu Gly Glu Lys Trp Lys Ser Arg Leu 2495 2500 2505 Asn Ala Leu Gly Lys Ser Glu Phe Gln Ile Tyr Lys Lys Ser Gly 2510 2515 2520 Ile Gln Glu Val Asp Arg Thr Leu Ala Lys Glu Gly Ile Lys Arg 2525 2530 2535 Gly Glu Thr Asp His His Ala Val Ser Arg Gly Ser Ala Lys Leu 2540 2545 2550 Arg Trp Phe Val Glu Arg Asn Met Val Thr Pro Glu Gly Lys Val 2555 2560 2565 Val Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Gly 2570 2575 2580 Gly Leu Lys Asn Val Arg Glu Val Lys Gly Leu Thr Lys Gly Gly 2585 2590 2595 Pro Gly His Glu Glu Pro Ile Pro Met Ser Thr Tyr Gly Trp Asn 2600 2605 2610 Leu Val Arg Leu Gln Ser Gly Val Asp Val Phe Phe Ile Pro Pro 2615 2620 2625 Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro 2630 2635 2640 Asn Pro Thr Val Glu Ala Gly Arg Thr Leu Arg Val Leu Asn Leu 2645 2650 2655 Val Glu Asn Trp Leu Asn Asn Asn Thr Gln Phe Cys Ile Lys Val 2660 2665 2670 Leu Asn Pro Tyr Met Pro Ser Val Ile Glu Lys Met Glu Ala Leu 2675 2680 2685 Gln Arg Lys Tyr Gly Gly Ala Leu Val Arg Asn Pro Leu Ser Arg 2690 2695 2700 Asn Ser Thr His Glu Met Tyr Trp Val Ser Asn Ala Ser Gly Asn 2705 2710 2715 Ile Val Ser Ser Val Asn Met Ile Ser Arg Met Leu Ile Asn Arg 2720 2725 2730 Phe Thr Met Arg Tyr Lys Lys Ala Thr Tyr Glu Pro Asp Val Asp 2735 2740 2745 Leu Gly Ser Gly Thr Arg Asn Ile Gly Ile Glu Ser Glu Ile Pro 2750 2755 2760 Asn Leu Asp Ile Ile Gly Lys Arg Ile Glu Lys Ile Lys Gln Glu 2765 2770 2775 His Glu Thr Ser Trp His Tyr Asp Gln Asp His Pro Tyr Lys Thr 2780 2785 2790 Trp Ala Tyr His Gly Ser Tyr Glu Thr Lys Gln Thr Gly Ser Ala 2795 2800 2805 Ser Ser Met Val Asn Gly Val Val Arg Leu Leu Thr Lys Pro Trp 2810 2815 2820 Asp Val Val Pro Met Val Thr Gln Met Ala Met Thr Asp Thr Thr 2825 2830 2835 Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg 2840 2845 2850 Thr Gln Glu Pro Lys Glu Gly Thr Lys Lys Leu Met Lys Ile Thr 2855 2860 2865 Ala Glu Trp Leu Trp Lys Glu Leu Gly Lys Lys Lys Thr Pro Arg 2870 2875 2880 Met Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn Ala 2885 2890 2895 Ala Leu Gly Ala Val Phe Thr Asp Glu Asn Lys Trp Lys Ser Ala 2900 2905 2910 Arg Glu Ala Val Glu Asp Ser Arg Phe Trp Glu Leu Val Asp Lys 2915 2920 2925 Glu Arg Asn Leu His Leu Glu Gly Lys Cys Glu Thr Cys Val Tyr 2930 2935 2940 Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys 2945 2950 2955 Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg 2960 2965 2970 Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp 2975 2980 2985 Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly Leu 2990 2995 3000 His Lys Leu Gly Tyr Ile Leu Arg Asp Val Ser Lys Lys Glu Gly 3005 3010 3015 Gly Ala Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile 3020 3025 3030 Thr Leu Glu Asp Leu Lys Asn Glu Glu Met Val Thr Asn His Met 3035 3040 3045 Glu Gly Glu His Lys Lys Leu Ala Glu Ala Ile Phe Lys Leu Thr 3050 3055 3060 Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Thr Pro Arg Gly 3065 3070 3075 Thr Val Met Asp Ile Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly 3080 3085 3090 Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala 3095 3100 3105 Gln Leu Ile Arg Gln Met Glu Gly Glu Gly Val Phe Lys Ser Ile 3110 3115 3120 Gln His Leu Thr Ile Thr Glu Glu Ile Ala Val Gln Asn Trp Leu 3125 3130 3135 Ala Arg Val Gly Arg Glu Arg Leu Ser Arg Met Ala Ile Ser Gly 3140 3145 3150 Asp Asp Cys Val Val Lys Pro Leu Asp Asp Arg Phc Ala Ser Ala 3155 3160 3165 Leu Thr Ala Leu Asn Asp Met Gly Lys Ile Arg Lys Asp Ile Gln 3170 3175 3180 Gln Trp Glu Pro Ser Arg Gly Trp Asn Asp Trp Thr Gln Val Pro 3185 3190 3195 Phe Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg 3200 3205 3210 Val Leu Val Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg 3215 3220 3225 Ala Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala 3230 3235 3240 Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe 3245 3250 3255 His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser Ala 3260 3265 3270 Val Pro Ser His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile 3275 3280 3285 His Ala Lys His Glu Trp Met Thr Thr Glu Asp Met Leu Thr Val 3290 3295 3300 Trp Asn Arg Val Trp Ile Gln Glu Asn Pro Trp Met Glu Asp Lys 3305 3310 3315 Thr Pro Val Glu Ser Trp Glu Glu Ile Pro Tyr Leu Gly Lys Arg 3320 3325 3330 Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala 3335 3340 3345 Thr Trp Ala Lys Asn Ile Gln Ala Ala Ile Asn Gln Val Arg Ser 3350 3355 3360 Leu Ile Gly Asn Glu Glu Tyr Thr Asp Tyr Met Pro Ser Met Lys 3365 3370 3375 Arg Phe Arg Arg Glu Glu Glu Glu Ala Gly Val Leu Trp 3380 3385 3390 <210>15 <211>10723 <212>DNA <213>Artificial Sequence <220> <BVS of Dengue virus serotype 2 <400>15 agttgttagt ctacgtggac cgacaaagac agattctttg agggagctaa gctcaatgta 60 gttctaacag ttttttaatt agagagcaga tctctgatga ataaccaacg gaaaaaggcg 120 aaaaacacgc ctttcaatat gctgaaacgc gagagaaacc gcgtgtcgac tgtgcaacag 180 ctgacaaaga gattctcact tggaatgctg cagggacgag gaccattaaa actgttcatg 240 gccctggtgg cgttccttcg tttcctaaca atcccaccaa cagcagggat attgaagaga 300 tggggaacaa ttaaaaaatc aaaagctatt aatgttttga gagggttcag gaaagagatt 360 ggaaggatgc tgaacatctt gaataggaga cgcagatctg caggcatgat cattatgctg 420 attccaacag tgatggcgtt ccatttaacc acacgtaacg gagaaccaca catgatcgtc 480 agcagacaag agaaagggaa aagtcttctg tttaaaacag aggttggcgt gaacatgtgt 540 accctcatgg ccatggacct tggtgaattg tgtgaagaca caatcacgta cgagtgtccc 600 cttctcaggc agaatgagcc agaagacata gactgttggt gcaactctac gtccacgtgg 660 gtaacttatg ggacgtgtac caccatggga gaacatagaa gagaaaaaag atcagtggca 720 ctcgttccac atgtgggaat gggactggag acacgaactg aaacatggat gtcatcagaa 780 ggggcctgga aacatgtcca gagaattgaa acttggatct tgagacatcc aggctcacc 840 atgatggcag caatcctggc atacaccata ggaacgacac atttccaag agccctgatc 900 ttcatcttac tgacagctgt cactccttca atgacaatgc gttgcatagg atgtcaaat 960 agagactttg tggaggggt tcaggagga agctggttg acatagtctt agaacatgga 1020 agctgtgtga cgacgatgc aaaaaaaaaaaacattgg attttgaact gataaaaaaca 1080 gagccaaac agcctgccac cctaggaag tactgtatag agggcaagct aaccaaca 1140 acaacagaat ctcgctgccc aacacaaggg gaacccagcc taaatgaga gcaggacaaa 1200 aggttcgtct gcaacactc catggtagac agaggatggg gaatggatg tggactattt 1260 ggaaagggag gcattgtgac ctgtgctatg ttcagatgca aaagaacat ggaaggaaaa 1320 gttgtgcaac cagaaaactt ggaatacacc attgtgataa cacctacactc agggaagg 1380 catgcagtcg gaatgacac aggaaacat ggcaaggaa tcaaataac accacagagt 1440 tccatcacag aagcagaatt gacaggttat ggcactgtca caatggagtg ctctccaaga 1500 acgggcctcg acttcaatga gatggtgttg ctgcagatgg aaaataagc ttggctggtg 1560 cacaggcaat ggttcctaga cctgccgtta ccatggttgc ccggagcgga cacaaggg 1620 tcaaattgga tacagaaaga gacattggtc actttcaaaa atccccatgc gaaaacag 1680 gatgttgttg ttttaggatc ccaagaaggg gccatgcaca cagcacttac aggggccaca 1740 gaaatccaaa tgtcatcagg aaacttactc ttcacaggac atctcaagtg caggctgaga 1800 1860 gtgaaggaaa tagcagaaac acaacatgga acaatagtta tcagagtgca atatgaaggg 1920 gacggctctc catgcaagat cccttttgag ataatggatt tggaaaaaag acatgtctta 1980 ggtcgcctga ttacagtcaa cccaattgtg acagaaaaag atagcccagt caacatagaa 2040 gcagaacctc catttggaga cagctacatc atcatagagg tagagccgggg aaactgaag 2100 ctcaactggt ttaagaaagg aagttctatc ggccaaatgt ttgagacaac aatgaggggg 2160 gcgaagagaa tggccattt aggtgacaca gctgggattttggttctt gggaggagtg 2220 tttacatcta taggaagggc tctccaccaa gtctttggag caatctatgg agctgccttc 2280 agtggggttt catggactt gaaaatccctc ataggagtca ttatcacatg gatggaatg 2340 aattcacgca gcacctcact gtctgtgaca ctagtattgg tgggaattgt jagactgtat 2400 ttgggagtca tggtgcaggc cgatagtggt tgcgttgtga gctggaaaaa caagaactg 2460 aaatgtggca gtgggattt catcacagac aacgtgcaca catggacaga acatacaag 2520 ttccaaccag aatccccttc aaaactagct tcagctatcc agaagccca tgaagaggac 2580 atttgtggaa tccgctcagt aacagactg gagaatctga tgtggaaca aaacacca 2640 gattgaatc acattctatc agaaaatgag gtgaagttaa ctattatgac aggagacatc 2700 aaaggaatca tgcaggcagg aaacgatct ctgcggccctc agcccactga gctgaagtat 2760 tcatggaaaa catggggcaa agcaaaatg ctctctacag agtctcataa ccagaccttt 2820 ctcattgatg gccccgaac agcagaatgc cccacaca atagagcttg gattcgttg 2880 gaagttgaag actatggctt tggagtattc accaccaata tattggctaaa attgaaagaa 2940 aaaacaggatg tattctgcga ctcaaactc atgtcagcgg ccaaaaga caacagagcc 3000 gtccatgccg attgggtta ttggatagaa agtgcactca atgacacatg gaagatagag 3060 aaagcctctt tcattgaagt taaaaactgc cactggccaa atcacacac cctctggagc 3120 aatggagtgc tagaagtga gatgatatt ccaagaatc tcgctggacc agtgtctca 3180 cacaactata gaccaggcta ccatacacaa atacaggac catggcatct aggtaagctt 3240 gagatgact tgatttctg tgatgacaca acagtgtag tgactgagga ctgcggaat 3300 agaggaccct ctttgagac aaccactgcc tctggaaac tcatacaga atggtgctgc 3360 cgatcttgca cattaccacc gctaagatac agaggtgagg atggttgctg gtacgggatg 3420 gaatcagac cattgaagga gaagagag aatttggtca actccttggt cacagctgga 3480 catgggcagg tcgacaactt ttcactagga gtcttggga tggcattgtt cctggaggaa 3540 atgcttagga cccgagtagg acgaaacat gcaatactac tagttgcagt ttctttgtg 3600 acattgatca cagggaacat gtcctttaga gacctgggaa gagtgatggt tatggtaggc 3660 gccactatga cggatgacat aggtatgggc gtgacttatc ttgccctact agcagccttc 3720 aaagtcagac caacttttgc agctggacta ctcttgagaa agctgacctc caaggaattg 3780 atgatgacta ctataggaat tgtactcctc tcccagagca ccataccaga gaccattctt 3840 gagttgactg atgcgttagc cttaggcatg atggtcctca aaatggtgag aaatatggaa 3900 aagtatcaat tggcagtgac tatcatggct atcttgtgcg tcccaaacgc agtgatatta 3960 caaaacgcat ggaaagtgag ttgcacaata ttggcagtgg tgtccgtttc cccactgttc 4020 ttaacatcct cacagcaaaa aacagattgg ataccattag cattgacgat caaaggtctc 4080 aatccaacag ctatttttct aacaaccctc tcaagaacca gcaagaaaag gagctggcca 4140 ttaaatgagg ctatcatggc agtcgggatg gtgagcattt tagccagttc tctcctaaaa 4200 aatgatattc ccatgacagg accattagtg gctggagggc tcctcactgt gtgctacgtg 4260 ctcactggac gatcggccga tttggaactg gagagagcag ccgatgtcaa atgggaagac 4320 4380. 4380. 4380. 4380. 4380. 4380. 4380. 4380. 4380 atgtcgataa aaaatgaaga ggaagaacaa acactgacca tactcattag aacaggattg ctggtgatct caggactttt tcctgtatca attack cggcagcagc atggtacctg tgggaagtga agaaacaacg ggccggagta ttgtgggatg ttccttcacc cccacccatg ggaaaggctg aactggaaga tggagcctat agaattaagc aaaaagggat tcttggatat tcccagatcg gagccggagt ttacaaagaa ggaacattcc atacaatgtg gcatgtcaca cgtggcgctg ttctaatgca taaaggaaag aggattgaac catcatgggc ggacgtcaag aaagacctaa tatcatatgg aggaggctgg aagttagaag gagaatgga ggaggaga gagtccagg tattggcact gagcctgga aaaaatcca gagccgtcca aacgaaacct ggtcttttca aaaccaacgc cggaacaata ggtgctgtat ctctggactt ttctcctgga 4980. acgtcaggat ctccaattat cgacaaaaaa ggaaaagttg tgggtcttta tggtaatggt gttgttacaa ggagtggagc atatgtgagt gctatagccc agactgaaaa aagcattgaa gaacccag agatcgaga tgacattttc cgaaagagaa gactgaccat catggaccctc 5100 cacccaggag cgggaagac gagagatac cttccggcca tagtcagaga agcttaaaa 5160 cggggtttga gacattaat cttggccccc actagagttg tggcagctga atggaggaa 5220 gcccttagag gacttccaat aagataccag accccagcca tcagagctgt gcacaccggg 5280 cgggagatg tggacctaat gtgtcatgcc accattacca tgaggctgct atcaccagtt 5340 agagtgccaa actacaacct gattatcatg gacgaagccc atttcacaga cccagcaagt 5400 atagcagcta gaggatacat ctcactcga gtggagatgg gtgaggcagc tgggattttt 5460 atgacagcca ctcccccgggg aagcagagac ccattccctc agagcaatgc accaatcata 5520 gatgagaaa gagaatccc tgaacgctcg tggaattccg gatgaatg ggtcacggat 5580 tttaaaggga agactgttg gttcgttcca agtataaaag caggaatga tatagcagct 5640 tgcctgagga aaatggaaa gaagtgata caaccagta ggaagacctt tgattctgag 5700 tatgtcaaga ctagaaccaa tgatgggac ttcgtggtta caactgacat ttcagaatg 5760 ggtgccaatt tcaaggctga gagggttata gaccccagac gctgcatgaa accagtcata 5820 ctaacagatg gtgaagagcg ggtgattctg gcaggaccta tgccagtgac ccactctagt 5880 gcagcacaaa gaagggag aataggaaga aatccaaaaa atgagaatga ccagtacata 5940 6000 ctcctagata acatcaacac gccagaagga atcattccta gcatgttcga accagagcgt 6060 gaaaaggtgg atgccattga tggcgaatac cgcttgagag gagaagcaag gaaaaccttt 6120 gtagacttaa tgagaagagg agacctacca gtctggttgg cctacagagt ggcagctgaa 6180 ggcatcaact acgcagacag aaggtggtgt tttgatggag tcaagaacaa ccaaatccta 6240 gaaaaacg tggaagttga aatctggaca aaagaagggg aaaggaagaa attgaaaccc 6300 agatggttgg atgctaggat ctattctgac ccactggcgc taaaagaatt taggaattt 6360 gcagccggaa gaaagtctct gaccctgaac ctaatcacag aaatgggtag gctcccaacc 6420 ttcatgactc agaagcaag agacgcactg gacaacttag cagtgctgca cacggctgag 6480 ccaggtggaa gggcgtacaa ccatgctctc agtgaactgc cggagaccct ggagacattg 6540 cttttactga cacttctggc tacagtcacg ggagggatct ttttattctt gatgagcgca 6600 aggggcatag ggaagatgac cctgggaatg tgctgcataa tcacggctag catcctccta 6660 tggtacgcac aaatacagcc acactggata gcagcttcaa taatactgga gttttttctc 6720 atagttttgc ttattccaga acctgaaaaa cagagaacac cccaagacaa ccaactgacc 6780 tacgttgtca tagccatcct cacagtggtg gccgcaacca tggcaaacga gatgggtttc 6840 ctagaaaaaa cgaagaaaga tctcggattg ggaagcattg caacccagca acccgagagc 6900 aacatcctgg acatagatct acgtcctgca tcagcatgga cgctgtatgc cgtggccaca 6960 acatttgtta caccaatgtt gagacatagc attgaaaatt cctcagtgaa tgtgtcccta 7020 acagctatag ccaaccaagc cacagtgtta atgggtctcg ggaaaggatg gccattgtca 7080 aagatggaca tcggagttcc ccttctcgcc attggatgct actcacaagt caaccccata 7140 actctcacag cagctttttt cttattggta gcacattatg ccatcatagg gccaggactc 7200 caagcaaag caccagaa agctcagaaa agagcagcgg cgggcatcat gaaaaaccca 7260 actgtcgatg gataacagt gattgaccta gatccaatac cttatgatcc aaagtttgaa 7320 aagcagttgg gatagtaat gctcctagtc ctctgcgtga ctcaagtatt gatgatgagg 7380 actacatggg ctctgtgtga ggctttaacc ttagctaccg ggcccactc cacattgtgg 7440 gaaggaaatc cagggaggtt ttggacact accattgcgg tgtcaatggc tacattttt 7500 agagggagtt acttggccgg agctggactt ctctttcta ttatgagaa cacaccac 7560 acaagaaggg gaactggcaa cataggagag acgcttggag agaatggaa aagccgattg 7620 aacgcattgg gaaaaagtga attccagatc tacaagaaa gtggaatcca ggaagtggat 7680 agaaccttag caaagaagg cattaaaaga ggagaaacgg accacacgc tgtgtcgcga 7740 ggctcagcaa aactgagatg gttcgttgag agaaacatgg tcacaccaga agggaagta 7800 gtggacctcg gttgtggcag aggaggctgg tcatactatt gtggaggact aagaatgta 7860 agagaagtca aaggcctaac aaaaggagga ccaggacacg aagacccat ccccatgtca 7920 acatatgggt ggaatctagt gcgtcttcaa agtggagttg acgttttctt catcccgcca 7980 gaaaagtgtg acacattatt gtgtgacata ggggagtcat caccaaatcc cacagtggaa 8040 gcaggacgaa cactcagagt ccttaactta gtagaaaatt ggttgaacaa caacactcaa 8100 ttttgcataa aggttctcaa cccatatatg ccctcagtca tagaaaaaat ggaagcacta 8160 caaaggaaat atggaggagc cttagtgagg aatccactct cacgaaactc cacacatgag 8220 atgtactggg tatccaatgc ttccgggaac atagtgtcat cagtgaacat gatttcaagg 8280 atgttgatca acagatttac aatgagatac aagaaagcca cttacgagcc ggatgttgac 8340 ctcggaagcg gaacccgtaa catcgggatt gaaagtgaga taccaaacct agatataatt 8400 gggaaaagaa tagaaaaat aaagcaagag catgaaacat catggcacta tgaccaagac 8460 cacccataca aaacgtgggc ataccatggt agctatgaaa caaaacagac tggatcagca 8520 tcatccatgg tcaacggagt ggtcaggctg ctgacaaaac cttgggacgt cgtccccatg 8580 gtgacacaga tggcaatgac agacacgact ccatttggac aacagcgcgt ttttaaagag 8640 aaagtggaca cgagaaccca agaaccgaa gaggcacga agaactaat gaaaataca 8700 gcagagtggc tttggaaga attaggaag aaaagacac ccaggatgtg caccagagaa 8760 gattcacaa gaaggtgag aagcaatgca gccttgggggg ccgtattcac tgatgagaac 8820 aagtggaagt cggcacgtga ggctgttgaa gatagtaggt tttgggagct ggttgacaag 8880 gaaaggaatc tccatcttga aggaaagtgt gaaacatgtg tgtacaacat gatgggaaaa 8940 aggagaga agctagggga attcggcaag gcaaaggca gcagagccat atggtacatg 9000 tggcttggag cacgcttctt agagtttga gccctaggat tctttaatga agatcactgg 9060 ttctccagag agaactccct gagtggagtg gaaggagaag ggctgcacaa gctaggttac 9120 attctaagag acgtgagcaa gaagaggga ggagcaatgt atgccgatga caccgcagga 9180 tgggatacaa gatcacacact agaagaccta aaaaatgaag aaatggtaac aaaccacatg 9240 gaaggagaac acaagaact agccgaggcc atttcaac taacgtacca aaacaggtg 9300 gtgcgtgtgc aaagaccaac accaagaggc acagtaatgg acatcatatc gagagagac 9360 haaagaggta gtggacaagt tggcacctat ggactcaata ctttcaccaa tatggaagcc 9420 caactaatca gagatgga gggagaagga gtctttaaaa gcattcagca cctaacaatc 9480 acagaagaaa tcgctgtgca aaactggtta gcaagagtgg ggcgcgaaag gttatcaaga 9540 atggccatca gtggagatga ttgtgttgtg aaacctttag atgacaggtt cgcaagcgct 9600 ttaacagctc taaatgacat gggaagatt aggaagaca tacacaatg ggaaccttca 9660 agaggatgga atgattggac acaagtgccc ttctgttcac accattcca tgagttaatc 9720 atgaaagacg gtcgcgtact cgttgttcca tgtagaaacc aagatgaact gattggcaga 9780 gcccgaatct cccaaggagc agggtggtct ttgcgggaga cggcctgtttt ggggaagtct 9840 tacgcccaaa tgtggagctt gatgtacttc cacagacgcg acctcaggct ggcggcaaat 9900 gctatttgct cggcagtacc atcacattgg gttccacaa gtcgacaac ctggtccata 9960 catgctaaac atgaatggat vakaacggaa gatatgctga cagtctggaa cagggtgtgg 10020 attcaagaaa acccatggat ggaagacaaa actccagtgg aatcatggga ggaatccca 10080 tacttgggga aaagagaaga ccaatggtgc ggctcattga ttgggttaac aagcagggcc 10140 acctgggcaa agaacatcca agcagcaata aatcaagtta gatcccttat aggcaatgaa 10200 gaatacacag attacatgcc atccatgaaa agattcagaa gagaagagga agaagcagga 10260 gttctgtggt agaaagcaaa actaacatga aacaaggcta gaagtcaggt cggattaagc 10320 catagtacgg aaaaaactat gctacctgtg agccccgtcc aaggacgtta aaagaagtca 10380 ggccatcata aatgccatag cttgagtaaa ctatgcagcc tgtagctcca cctgagaagg 10440 tgtaaaaaat ccgggaggcc acaaaccatg gaagctgtac gcatggcgta gtggactagc 10500 ggttagagga gacccctccc ttacaaatcg cagcaacaat gggggcccaa ggcgagatga 10560 agctgtagtc tcgctggaag gactagaggt tagaggagac ccccccgaaa caaaaaacag 10620 catattgacg ctgggaaaga ccagagatcc tgctgtctcc tcagcatcat tccaggcaca 10680 gaacgccaga aaatggaatg gtgctgttga atcaacaggt tct 10723 <210>16 <211>3391 <212>PRT <213>Artificial Sequence <220> <223>Dengue virus serotype 2, BVS <400>16 Met Asn Asn Gln Arg Lys Lys Ala Lys Asn Thr Pro Phe Asn Met Leu 1 5 10 15 Lys Arg Glu Arg Asn Arg Val Ser Thr Val Gln Gln Leu Thr Lys Arg 20 25 30 Phe Ser Leu Gly Met Leu Gln Gly Arg Gly Pro Leu Lys Leu Phe Met 35 40 45 Ala Leu Val Ala Phe Leu Arg Phe Leu Thr Ile Pro Pro Thr Ala Gly 50 55 60 Ile Leu Lys Arg Trp Gly Thr Ile Lys Lys Ser Lys Ala Ile Asn Val 65 70 75 80 Leu Arg Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn 85 90 95 Arg Arg Arg Arg Ser Ala Gly Met Ile Ile Met Leu Ile Pro Thr Val 100 105 110 Met Ala Phe His Leu Thr Thr Arg Asn Gly Glu Pro His Met Ile Val 115 120 125 Ser Arg Gln Glu Lys Gly Lys Ser Leu Leu Phe Lys Thr Glu Val Gly 130 135 140 Val Asn Met Cys Thr Leu Met Ala Met Asp Leu Gly Glu Leu Cys Glu 145 150 155 160 Asp Thr Ile Thr Tyr Glu Cys Pro Leu Leu Arg Gln Asn Glu Pro Glu 165 170 175 Asp Ile Asp Cys Trp Cys Asn Ser Thr Ser Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Thr Thr Met Gly Glu His Arg Arg Glu Lys Arg Ser Val Ala 195 200 205 Leu Val Pro His Val Gly Met Gly Leu Glu Thr Arg Thr Glu Thr Trp 210 215 220 Met Ser Ser Glu Gly Ala Trp Lys His Val Gln Arg Ile Glu Thr Trp 225 230 235 240 Ile Leu Arg His Pro Gly Phe Thr Met Met Ala Ala Ile Leu Ala Tyr 245 250 255 Thr Ile Gly Thr Thr His Phe Gln Arg Ala Leu Ile Phe Ile Leu Leu 260 265 270 Thr Ala Val Thr Pro Ser Met Thr Met Arg Cys Ile Gly Met Ser Asn 275 280 285 Arg Asp Phe Val Glu Gly Val Ser Gly Gly Ser Trp Val Asp Ile Val 290 295 300 Leu Glu His Gly Ser Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Phe Glu Leu Ile Lys Thr Glu Ala Lys Gln Pro Ala Thr Leu 325 330 335 Arg Lys Tyr Cys Ile Glu Ala Lys Leu Thr Asn Thr Thr Thr Glu Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Pro Ser Leu Asn Glu Glu Gln Asp Lys 355 360 365 Arg Phe Val Cys Lys His Ser Met Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Gly Ile Val Thr Cys Ala Met Phe Arg 385 390 395 400 Cys Lys Lys Asn Met Glu Gly Lys Val Val Gln Pro Glu Asn Leu Glu 405 410 415 Tyr Thr Ile Val Ile Thr Pro His Ser Gly Glu Glu His Ala Val Gly 420 425 430 Asn Asp Thr Gly Lys His Gly Lys Glu Ile Lys Ile Thr Pro Gln Ser 435 440 445 Ser Ile Thr Glu Ala Glu Leu Thr Gly Tyr Gly Thr Val Thr Met Glu 450 455 460 Cys Ser Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Val Leu Leu Gln 465 470 475 480 Met Glu Asn Lys Ala Trp Leu Val His Arg Gln Trp Phe Leu Asp Leu 485 490 495 Pro Leu Pro Trp Leu Pro Gly Ala Asp Thr Gln Gly Ser Asn Trp Ile 500 505 510 Gln Lys Glu Thr Leu Val Thr Phe Lys Asn Pro His Ala Lys Lys Gln 515 520 525 Asp Val Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu 530 535 540 Thr Gly Ala Thr Glu Ile Gln Met Ser Ser Gly Asn Leu Leu Phe Thr 545 550 555 560 Gly His Leu Lys Cys Arg Leu Arg Met Asp Lys Leu Gln Leu Lys Gly 565 570 575 Met Ser Tyr Ser Met Cys Thr Gly Lys Phe Lys Val Val Lys Glu Ile 580 585 590 Ala Glu Thr Gln His Gly Thr Ile Val Ile Arg Val Gln Tyr Glu Gly 595 600 605 Asp Gly Ser Pro Cys Lys Ile Pro Phe Glu Ile Met Asp Leu Glu Lys 610 615 620 Arg His Val Leu Gly Arg Leu Ile Thr Val Asn Pro Ile Val Thr Glu 625 630 635 640 Lys Asp Ser Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Asp Ser 645 650 655 Tyr Ile Ile Ile Gly Val Glu Pro Gly Gln Leu Lys Leu Asn Trp Phe 660 665 670 Lys Lys Gly Ser Ser Ile Gly Gln Met Phe Glu Thr Thr Met Arg Gly 675 680 685 Ala Lys Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser 690 695 700 Leu Gly Gly Val Phe Thr Ser Ile Gly Lys Ala Leu His Gln Val Phe 705 710 715 720 Gly Ala Ile Tyr Gly Ala Ala Phe Ser Gly Val Ser Trp Thr Met Lys 725 730 735 Ile Leu Ile Gly Val Ile Ile Thr Trp Ile Gly Met Asn Ser Arg Ser 740 745 750 Thr Ser Leu Ser Val Thr Leu Val Leu Val Gly Ile Val Thr Leu Tyr 755 760 765 Leu Gly Val Met Val Gln Ala Asp Ser Gly Cys Val Val Ser Trp Lys 770 775 780 Asn Lys Glu Leu Lys Cys Gly Ser Gly Ile Phe Ile Thr Asp Asn Val 785 790 795 800 His Thr Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ser Lys 805 810 815 Leu Ala Ser Ala Ile Gln Lys Ala His Glu Glu Asp Ile Cys Gly Ile 820 825 830 Arg Ser Val Thr Arg Leu Glu Asn Leu Met Trp Lys Gln Ile Thr Pro 835 840 845 Glu Leu Asn His Ile Leu Ser Glu Asn Glu Val Lys Leu Thr Ile Met 850 855 860 Thr Gly Asp Ile Lys Gly Ile Met Gln Ala Gly Lys Arg Ser Leu Arg 865 870 875 880 Pro Gln Pro Thr Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala 885 890 895 Lys Met Leu Ser Thr Glu Ser His Asn Gln Thr Phe Leu Ile Asp Gly 900 905 910 Pro Glu Thr Ala Glu Cys Pro Asn Thr Asn Arg Ala Trp Asn Ser Leu 915 920 925 Glu Val Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu 930 935 940 Lys Leu Lys Glu Lys Gln Asp Val Phe Cys Asp Ser Lys Leu Met Ser 945 950 955 960 Ala Ala Ile Lys Asp Asn Arg Ala Val His Ala Asp Met Gly Tyr Trp 965 970 975 Ile Glu Ser Ala Leu Asn Asp Thr Trp Lys Ile Glu Lys Ala Ser Phe 980 985 990 Ile Glu Val Lys Asn Cys His Trp Pro Lys Ser His Thr Leu Trp Ser 995 1000 1005 Asn Gly Val Leu Glu Ser Glu Met Ile Ile Pro Lys Asn Leu Ala 1010 1015 1020 Gly Pro Val Ser Gln His Asn Tyr Arg Pro Gly Tyr His Thr Gln 1025 1030 1035 Ile Thr Gly Pro Trp His Leu Gly Lys Leu Glu Met Asp Phe Asp 1040 1045 1050 Phe Cys Asp Gly Thr Thr Val Val Val Thr Glu Asp Cys Gly Asn 1055 1060 1065 Arg Gly Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Ile 1070 1075 1080 Thr Glu Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr 1085 1090 1095 Arg Gly Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu 1100 1105 1110 Lys Glu Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala Gly 1115 1120 1125 His Gly Gln Val Asp Asn Phe Ser Leu Gly Val Leu Gly Met Ala 1130 1135 1140 Leu Phe Leu Glu Glu Met Leu Arg Thr Arg Val Gly Thr Lys His 1145 1150 1155 Ala Ile Leu Leu Val Ala Val Ser Phe Val Thr Leu Ile Thr Gly 1160 1165 1170 Asn Met Ser Phe Arg Asp Leu Gly Arg Val Met Val Met Val Gly 1175 1180 1185 Ala Thr Met Thr Asp Asp Ile Gly Met Gly Val Thr Tyr Leu Ala 1190 1195 1200 Leu Leu Ala Ala Phe Lys Val Arg Pro Thr Phe Ala Ala Gly Leu 1205 1210 1215 Leu Leu Arg Lys Leu Thr Ser Lys Glu Leu Met Met Thr Thr Ile 1220 1225 1230 Gly Ile Val Leu Leu Ser Gln Ser Thr Ile Pro Glu Thr Ile Leu 1235 1240 1245 Glu Leu Thr Asp Ala Leu Ala Leu Gly Met Met Val Leu Lys Met 1250 1255 1260 Val Arg Asn Met Glu Lys Tyr Gln Leu Ala Val Thr Ile Met Ala 1265 1270 1275 Ile Leu Cys Val Pro Asn Ala Val Ile Leu Gln Asn Ala Trp Lys 1280 1285 1290 Val Ser Cys Thr Ile Leu Ala Val Val Ser Val Ser Pro Leu Phe 1295 1300 1305 Leu Thr Ser Ser Gln Gln Lys Thr Asp Trp Ile Pro Leu Ala Leu 1310 1315 1320 Thr Ile Lys Gly Leu Asn Pro Thr Ala Ile Phe Leu Thr Thr Leu 1325 1330 1335 Ser Arg Thr Ser Lys Lys Arg Ser Trp Pro Leu Asn Glu Ala Ile 1340 1345 1350 Met Ala Val Gly Met Val Ser Ile Leu Ala Ser Ser Leu Leu Lys 1355 1360 1365 Asn Asp Ile Pro Met Thr Gly Pro Leu Val Ala Gly Gly Leu Leu 1370 1375 1380 Thr Val Cys Tyr Val Leu Thr Gly Arg Ser Ala Asp Leu Glu Leu 1385 1390 1395 Glu Arg Ala Ala Asp Val Lys Trp Glu Asp Gln Ala Glu Ile Ser 1400 1405 1410 Gly Ser Ser Pro Ile Leu Ser Ile Thr Ile Ser Glu Asp Gly Ser 1415 1420 1425 Met Ser Ile Lys Asn Glu Glu Glu Glu Gln Thr Leu Thr Ile Leu 1430 1435 1440 Ile Arg Thr Gly Leu Leu Val Ile Ser Gly Leu Phe Pro Val Ser 1445 1450 1455 Ile Pro Ile Thr Ala Ala Ala Trp Tyr Leu Trp Glu Val Lys Lys 1460 1465 1470 Gln Arg Ala Gly Val Leu Trp Asp Val Pro Ser Pro Pro Pro Met 1475 1480 1485 Gly Lys Ala Glu Leu Glu Asp Gly Ala Tyr Arg Ile Lys Gln Lys 1490 1495 1500 Gly Ile Leu Gly Tyr Ser Gln Ile Gly Ala Gly Val Tyr Lys Glu 1505 1510 1515 Gly Thr Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu 1520 1525 1530 Met His Lys Gly Lys Arg Ile Glu Pro Ser Trp Ala Asp Val Lys 1535 1540 1545 Lys Asp Leu Ile Ser Tyr Gly Gly Gly Trp Lys Leu Glu Gly Glu 1550 1555 1560 Trp Lys Glu Gly Glu Glu Val Gln Val Leu Ala Leu Glu Pro Gly 1565 1570 1575 Lys Asn Pro Arg Ala Val Gln Thr Lys Pro Gly Leu Phe Lys Thr 1580 1585 1590 Asn Ala Gly Thr Ile Gly Ala Val Ser Leu Asp Phe Ser Pro Gly 1595 1600 1605 Thr Ser Gly Ser Pro Ile Ile Asp Lys Lys Gly Lys Val Val Gly 1610 1615 1620 Leu Tyr Gly Asn Gly Val Val Thr Arg Ser Gly Ala Tyr Val Ser 1625 1630 1635 Ala Ile Ala Gln Thr Glu Lys Ser Ile Glu Asp Asn Pro Glu Ile 1640 1645 1650 Glu Asp Asp Ile Phe Arg Lys Arg Arg Leu Thr Ile Met Asp Leu 1655 1660 1665 His Pro Gly Ala Gly Lys Thr Lys Arg Tyr Leu Pro Ala Ile Val 1670 1675 1680 Arg Glu Ala Ile Lys Arg Gly Leu Arg Thr Leu Ile Leu Ala Pro 1685 1690 1695 Thr Arg Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu 1700 1705 1710 Pro Ile Arg Tyr Gln Thr Pro Ala Ile Arg Ala Val His Thr Gly 1715 1720 1725 Arg Glu Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg 1730 1735 1740 Leu Leu Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met 1745 1750 1755 Asp Glu Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly 1760 1765 1770 Tyr Ile Ser Thr Arg Val Glu Met Gly Glu Ala Ala Gly Ile Phe 1775 1780 1785 Met Thr Ala Thr Pro Pro Gly Ser Arg Asp Pro Phe Pro Gln Ser 1790 1795 1800 Asn Ala Pro Ile Ile Asp Glu Glu Arg Glu Ile Pro Glu Arg Ser 1805 1810 1815 Trp Asn Ser Gly His Glu Trp Val Thr Asp Phe Lys Gly Lys Thr 1820 1825 1830 Val Trp Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Ala 1835 1840 1845 Cys Leu Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys 1850 1855 1860 Thr Phe Asp Ser Glu Tyr Val Lys Thr Arg Thr Asn Asp Trp Asp 1865 1870 1875 Phe Val Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys 1880 1885 1890 Ala Glu Arg Val Ile Asp Pro Arg Arg Cys Met Lys Pro Val Ile 1895 1900 1905 Leu Thr Asp Gly Glu Glu Arg Val Ile Leu Ala Gly Pro Met Pro 1910 1915 1920 Val Thr His Ser Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg 1925 1930 1935 Asn Pro Lys Asn Glu Asn Asp Gln Tyr Ile Tyr Met Gly Glu Pro 1940 1945 1950 Leu Glu Asn Asp Glu Asp Cys Ala His Trp Lys Glu Ala Lys Met 1955 1960 1965 Leu Leu Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ser Met 1970 1975 1980 Phe Glu Pro Glu Arg Glu Lys Val Asp Ala Ile Asp Gly Glu Tyr 1985 1990 1995 Arg Leu Arg Gly Glu Ala Arg Lys Thr Phe Val Asp Leu Met Arg 2000 2005 2010 Arg Gly Asp Leu Pro Val Trp Leu Ala Tyr Arg Val Ala Ala Glu 2015 2020 2025 Gly Ile Asn Tyr Ala Asp Arg Arg Trp Cys Phe Asp Gly Val Lys 2030 2035 2040 Asn Asn Gln Ile Leu Glu Glu Asn Val Glu Val Glu Ile Trp Thr 2045 2050 2055 Lys Glu Gly Glu Arg Lys Lys Leu Lys Pro Arg Trp Leu Asp Ala 2060 2065 2070 Arg Ile Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Glu Phe 2075 2080 2085 Ala Ala Gly Arg Lys Ser Leu Thr Leu Asn Leu Ile Thr Glu Met 2090 2095 2100 Gly Arg Leu Pro Thr Phe Met Thr Gln Lys Ala Arg Asp Ala Leu 2105 2110 2115 Asp Asn Leu Ala Val Leu His Thr Ala Glu Pro Gly Gly Arg Ala 2120 2125 2130 Tyr Asn His Ala Leu Ser Glu Leu Pro Glu Thr Leu Glu Thr Leu 2135 2140 2145 Leu Leu Leu Thr Leu Leu Ala Thr Val Thr Gly Gly Ile Phe Leu 2150 2155 2160 Phe Leu Met Ser Ala Arg Gly Ile Gly Lys Met Thr Leu Gly Met 2165 2170 2175 Cys Cys Ile Ile Thr Ala Ser Ile Leu Leu Trp Tyr Ala Gln Ile 2180 2185 2190 Gln Pro His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu 2195 2200 2205 Ile Val Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln 2210 2215 2220 Asp Asn Gln Leu Thr Tyr Val Val Ile Ala Ile Leu Thr Val Val 2225 2230 2235 Ala Ala Thr Met Ala Asn Glu Met Gly Phe Leu Glu Lys Thr Lys 2240 2245 2250 Lys Asp Leu Gly Leu Gly Ser Ile Ala Thr Gln Gln Pro Glu Ser 2255 2260 2265 Asn Ile Leu Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu 2270 2275 2280 Tyr Ala Val Ala Thr Thr Phe Val Thr Pro Met Leu Arg His Ser 2285 2290 2295 Ile Glu Asn Ser Ser Val Asn Val Ser Leu Thr Ala Ile Ala Asn 2300 2305 2310 Gln Ala Thr Val Leu Met Gly Leu Gly Lys Gly Trp Pro Leu Ser 2315 2320 2325 Lys Met Asp Ile Gly Val Pro Leu Leu Ala Ile Gly Cys Tyr Ser 2330 2335 2340 Gln Val Asn Pro Ile Thr Leu Thr Ala Ala Phe Phe Leu Leu Val 2345 2350 2355 Ala His Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr 2360 2365 2370 Arg Glu Ala Gln Lys Arg Ala Ala Ala Gly Ile Met Lys Asn Pro 2375 2380 2385 Thr Val Asp Gly Ile Thr Val Ile Asp Leu Asp Pro Ile Pro Tyr 2390 2395 2400 Asp Pro Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val 2405 2410 2415 Leu Cys ValThr Gln Val Leu Met Met Arg Thr Thr Trp Ala Leu 2420 2425 2430 Cys Glu Ala Leu Thr Leu Ala Thr Gly Pro Ile Ser Thr Leu Trp 2435 2440 2445 Glu Gly Asn Pro Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser 2450 2455 2460 Met Ala Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu 2465 2470 2475 Leu Phe Ser Ile Met Lys Asn Thr Thr Asn Thr Arg Arg Gly Thr 2480 2485 2490 Gly Asn Ile Gly Glu Thr Leu Gly Glu Lys Trp Lys Ser Arg Leu 2495 2500 2505 Asn Ala Leu Gly Lys Ser Glu Phe Gln Ile Tyr Lys Lys Ser Gly 2510 2515 2520 Ile Gln Glu Val Asp Arg Thr Leu Ala Lys Glu Gly Ile Lys Arg 2525 2530 2535 Gly Glu Thr Asp His His Ala Val Ser Arg Gly Ser Ala Lys Leu 2540 2545 2550 Arg Trp Phe Val Glu Arg Asn Met Val Thr Pro Glu Gly Lys Val 2555 2560 2565 Val Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Gly 2570 2575 2580 Gly Leu Lys Asn Val Arg Glu Val Lys Gly Leu Thr Lys Gly Gly 2585 2590 2595 Pro Gly His Glu Glu Pro Ile Pro Met Ser Thr Tyr Gly Trp Asn 2600 2605 2610 Leu Val Arg Leu Gln Ser Gly Val Asp Val Phe Phe Ile Pro Pro 2615 2620 2625 Glu Lys Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro 2630 2635 2640 Asn Pro Thr Val Glu Ala Gly Arg Thr Leu Arg Val Leu Asn Leu 2645 2650 2655 Val Glu Asn Trp Leu Asn Asn Asn Thr Gln Phe Cys Ile Lys Val 2660 2665 2670 Leu Asn Pro Tyr Met Pro Ser Val Ile Glu Lys Met Glu Ala Leu 2675 2680 2685 Gln Arg Lys Tyr Gly Gly Ala Leu ValArg Asn Pro Leu Ser Arg 2690 2695 2700 Asn Ser Thr His Glu Met Tyr Trp Val Ser Asn Ala Ser Gly Asn 2705 2710 2715 Ile Val Ser Ser Val Asn Met Ile Ser Arg Met Leu Ile Asn Arg 2720 2725 2730 Phe Thr Met Arg Tyr Lys Lys Ala Thr Tyr Glu Pro Asp Val Asp 2735 2740 2745 Leu Gly Ser Gly Thr Arg Asn Ile Gly Ile Glu Ser Glu Ile Pro 2750 2755 2760 Asn Leu Asp Ile Ile Gly Lys Arg Ile Glu Lys Ile Lys Gln Glu 2765 2770 2775 His Glu Thr Ser Trp His Tyr Asp Gln Asp His Pro Tyr Lys Thr 2780 2785 2790 Trp Ala Tyr His Gly Ser Tyr Glu Thr Lys Gln Thr Gly Ser Ala 2795 2800 2805 Ser Ser Met Val Asn Gly Val Val Arg Leu Leu Thr Lys Pro Trp 2810 2815 2820 Asp Val Val Pro Met Val Thr Gln Met Ala Met Thr Asp Thr Thr 2825 2830 2835 Pro Phe Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg 2840 2845 2850 Thr Gln Glu Pro Lys Glu Gly Thr Lys Lys Leu Met Lys Ile Thr 2855 2860 2865 Ala Glu Trp Leu Trp Lys Glu Leu Gly Lys Lys Lys Thr Pro Arg 2870 2875 2880 Met Cys Thr Arg Glu Glu Phe Thr Arg Lys Val Arg Ser Asn Ala 2885 2890 2895 Ala Leu Gly Ala Val Phe Thr Asp Glu Asn Lys Trp Lys Ser Ala 2900 2905 2910 Arg Glu Ala Val Glu Asp Ser Arg Phe Trp Glu Leu Val Asp Lys 2915 2920 2925 Glu Arg Asn Leu His Leu Glu Gly Lys Cys Glu Thr Cys Val Tyr 2930 2935 2940 Asn Met Met Gly Lys Arg Glu Lys Lys Leu Gly Glu Phe Gly Lys 2945 2950 2955 Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg 2960 2965 2970 Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp 2975 2980 2985 Phe Ser Arg Glu Asn Ser Leu Ser Gly Val Glu Gly Glu Gly Leu 2990 2995 3000 His Lys Leu Gly Tyr Ile Leu Arg Asp Val Ser Lys Lys Glu Gly 3005 3010 3015 Gly Ala Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile 3020 3025 3030 Thr Leu Glu Asp Leu Lys Asn Glu Glu Met Val Thr Asn His Met 3035 3040 3045 Glu Gly Glu His Lys Lys Leu Ala Glu Ala Ile Phe Lys Leu Thr 3050 3055 3060 Tyr Gln Asn Lys Val Val Arg Val Gln Arg Pro Thr Pro Arg Gly 3065 3070 3075 Thr Val Met Asp Ile Ile Ser Arg Arg Asp Gln Arg Gly Ser Gly 3080 3085 3090 Gln Val Gly Thr Tyr Gly Leu Asn Thr Phe Thr Asn Met Glu Ala 3095 3100 3105 Gln Leu Ile Arg Gln Met Glu Gly Glu Gly Val Phe Lys Ser Ile 3110 3115 3120 Gln His Leu Thr Ile Thr Glu Glu Ile Ala Val Gln Asn Trp Leu 3125 3130 3135 Ala Arg Val Gly Arg Glu Arg Leu Ser Arg Met Ala Ile Ser Gly 3140 3145 3150 Asp Asp Cys Val Val Lys Pro Leu Asp Asp Arg Phe Ala Ser Ala 3155 3160 3165 Leu Thr Ala Leu Asn Asp Met Gly Lys Ile Arg Lys Asp Ile Gln 3170 3175 3180 Gln Trp Glu Pro Ser Arg Gly Trp Asn Asp Trp Thr Gln Val Pro 3185 3190 3195 Phe Cys Ser His His Phe His Glu Leu Ile Met Lys Asp Gly Arg 3200 3205 3210 Val Leu Val Val Pro Cys Arg Asn Gln Asp Glu Leu Ile Gly Arg 3215 3220 3225 Ala Arg Ile Ser Gln Gly Ala Gly Trp Ser Leu Arg Glu Thr Ala 3230 3235 3240 Cys Leu Gly Lys Ser Tyr Ala Gln Met Trp Ser Leu Met Tyr Phe 3245 3250 3255 His Arg Arg Asp Leu Arg Leu Ala Ala Asn Ala Ile Cys Ser Ala 3260 3265 3270 Val Pro Ser His Trp Val Pro Thr Ser Arg Thr Thr Trp Ser Ile 3275 3280 3285 His Ala Lys His Glu Trp Met Thr Thr Glu Asp Met Leu Thr Val 3290 3295 3300 Trp Asn Arg Val Trp Ile Gln Glu Asn Pro Trp Met Glu Asp Lys 3305 3310 3315 Thr Pro Val Glu Ser Trp Glu Glu Ile Pro Tyr Leu Gly Lys Arg 3320 3325 3330 Glu Asp Gln Trp Cys Gly Ser Leu Ile Gly Leu Thr Ser Arg Ala 3335 3340 3345 Thr Trp Ala Lys Asn Ile Gln Ala Ala Ile Asn Gln Val Arg Ser 3350 3355 3360 Leu Ile Gly Asn Glu Glu Tyr Thr Asp Tyr Met Pro Ser Met Lys 3365 3370 3375 Arg Phe Arg Arg Glu Glu Glu Glu Ala Gly Val Leu Trp 3380 3385 3390 <210> 17 <211> 10717 <212> DNA <213> artificial sequence <220> <223> Dengue fever virus serotype 3, the main one <400> 17 agttgttagt ctacgtggac cgacaaagac agattctttg agggagctaa gctcaatgta 60 gttctaacag ttttttaatt agagacaga tctctgatga ataaccaacg gaaaaaggcg 120 aaaaaacacgc ctttcaatat gctgaaacgc gagagaaacc gcgtgtcgac tgtgcaacag 180 ctgacaaaga gattctcact tggaatgctg cagggacgag gaccattaaa actgttcatg 240 gccctggtgg cgttccttcg tttcctaaca atcccaccaa cagcagggat attgaagaga 300 tggggaacaa ttaaaaaatc aaaagctatt aatgttttga gagggttcag gaaagagatt 360 ggaaggatgc tgaacatctt gaataggaga cgcagatctg caggcatgat cattatgctg 420 attccaacag tgatggcgtt ccatttaacc acgcgtgatg gagagccgcg catgattgtg 480 gggaagaatg aaagaggaaa atccctactt ttcaagacag cctctggaat caacatgtgc 540 acactcatag ctatggatct gggagagatg tgtgatgaca cggtcactta caaatgcccc 600 cacattaccg aagtggagcc tgaagacatt gactgctggt gcaaccttac atcgacatgg 660 gtgacttatg gaacatgcaa tcaagctgga gagcatagac gcgataagag atcagtggcg 720 ttagctcccc atgttggcat gggactggac acacgcactc aaacctggat gtcggctgaa 780 ggagcttgga gacaagtcga gaaggtagag acatgggccc ttaggcaccc agggtttacc 840 atactagccc tatttcttgc ccattacata ggcacttcct tgacccagaa agtggttatt 900 tttatactat taatgctggt taccccatcc atgacaatga gatgtgtagg agtaggaaac 960 agagattttg tggaaggcct atcgggagct acgtgggttg acgtggtgct cgagcacggt 1020 gggtgtgtga ctaccatggc taggaacaag cccacgctgg acatagagct tcagaagacc 1080 gaggccaccc aactggcgac cctaggaag ctagcattg agggaaaat taccacata 1140 acaacgact cagatgtcc cacccaaggg gaagcgattt tacctgagga gcaggaccag 1200 aactacgtgt gtaagcatac atacgtggac agaggctggg gaaacggttg tggttgttt 1260 ggcaagggaa gcttggtgac atgcgcgaaa ttcaatgtt tagaatcaat agagggaaaa 1320 gtggtgcaac atgagaacct aaatacacc gtcatcatca cagtgcacac aggagaccaa 1380 caccaggtgg gaatgaac gcagggagtc acggctgaga taacacccca ggcatcacc 1440 gctgaagcca ttttacctga atatggacc ctcgggctag atgctcacc acggacaggt 1500 ttggatttca atgaatgat ctcattgaca atgagaca aagcatggat ggtacataga 1560 caatggttct ttgacttacc cctaccatgg acatcaggag cttcagcaga acaccaact 1620 tggaacagga agagcttct tgtgacatttt aaaaatgcac atgcaaaaaa gcagaagta 1680 gttgttcttg gatcacaaga gggagcaatg catacagcac tgacaggagc tacagagatc 1740 1800 aaattggaac tcaaggggat gagctatgca atgtgcttga gtagctttgt gttgaagaaa 1860 gaagtctccg aaacgcagca tgggacaata ctcattaagg ttgagtacaa aggggagaat 1920 gcaccctgca agattccttt ctccacggag gatggacaag gaaagctct caatggcaga 1980 ctgatcacag ccaatccagt ggtgaccaag aaggaggac ctgtcaacat tgaggctgaa 2040 cctccttttg gagaagtaa catagtaatt ggaattggag acaaagccct gaaaatcaac 2100 tggtacaaga agggaagctc gattgggaag atgttcgagg ccactgccag aggtgcaagg 2160 cgcatggcca tcttgggaga cacagcctgg gactttggat cagtgggtgg tgttttgaat 2220 tcattaggga aaatggtcca ccaaatattt gggagtgctt acacagccct atttggtgga 2280 gtctcctgga tgatgaaaat tggaataggt gtcctcttaa cctggatagg gttgaactca 2340 aaaaatactt ctatgtcatt ttcatgcatc gcggccggca ttgtgacact gtatttggga 2400 gtcatggtgc aggccgatag tggttgcgtt gtgagctgga aaaaaaga actgaaatgt 2460 ggcagtggga ttttcatcac agacaacgtg cacacatgga cagaacaata caagttccaa 2520 ccagaatccc cttcaaaact agcttcagct atccagaaag cccatgaaga ggacatttgt 2580 ggaatccgct cattacaag actggagaat ctgatgtgga aacaataac accagaattg 2640 aatcacattc tatcagaaaa tgaggtgaag ttaactatta tgacaggaga catcaaaagga 2700 atcatgcagg caggaaaacg atctctgcgg cctcagccca ctgagctgaa gtattcatgg 2760 aaaacatggg gcaaagcaaa aatgctctct acagagtctc ataaccagac ctttctcatt 2820 gatggccccg aaacagcaga atgccccaac acaaatagag cttggaattc gttggaagtt 2880 gaagaactatg gctttggagt attcaccacc aatatatggc taaaattgaa agaaaaacag 2940 gatgtattct gcgactcaaa actcatgtca gcggccataa aagaacacag agccgtccat 3000 gccgatatgg gttattggat agaaagtgca ctcaatgaca catggagaat agaaaagcc 3060 tctttcattg aagttaaaaa ctgccactgg ccaaaatcac acaccctctg gagcaatgga 3120 gtgctagaaa gtgagatgat aattccaaag aatctcgctg gaccagtgtc tcaacacaac 3180 tatagaccag gctaccatac acaaataca ggaccatggc atctaggtaa gcttgagatg 3240 gactttgatt tctgtgatgg aacagtg gtagtgactg aggactgcgg aaatagagga 3300 ccctcttga gaacaccac tgcctgga aactcataa cagaatggtg ctgccgatct 3360 tgcacattac caccgctaag atacagaggt gaggatgggt gctggtacgg gatggaatc 3420 agaccattga aggagaaga agaatttg gtcactcct tggtcacagc tggacatggg 3480 caggtcgaca actttcact aggagtctg ggaatggcat tgttcctgga ggaatgctt 3540 aggacccgag taggaacgaa acatgcaata ctactagttg cagttttctt tgtgacattg 3600 atcacaggga acatgtcctt tagagactg ggaagagtga tggttatggt aggcgccact 3660 atgacggatg acataggtat gggcgtgact tatcttgccc tactagcagc cttcaagtc 3720 agaccaactt ttgcagctgg actactcttg agaaagctga cctccaagga attgatgatg 3780 actactatag gattgtact cctctcccag agcaccatac cagagaccat tcttgagttg 3840 actgatgcgt tagccttagg catgatggtc ctcaaatgg tgagaataat ggaaagtat 3900 cavattggcag tgactcat ggctatcttg tgcgtcccaa acgcagtgat attacaaac 3960 gcatggaaag tgagttgcac atattggca gtggtgtccg ttcccact gttctttaca 4020 tcctcacagc aaaaaaacaga ttggatacca ttagcattga cgatcaagg tctcaatcca 4080 acagctattt ttctacaac cctctcaaga accagcaga aaggagctg gccattaat 4140 gaggctatca tggcagtcgg gatggtgagc attagcca gttctctcct aaaaaatgat 4200 attcccatga caggaccatt agtggctgga gggctcctca ctgtgtgcta cgtgctcact 4260 ggacgatcgg ccgatttgga actggagaga gcagccgatg tcaatggga agaccaggca 4320 gagatatcag gaagcagtcc aatcctgtca atacaat cagaatgg tagcatgtcg 4380 aaaaaatg aagaggaga aaaacactg accatactca ttagaacagg attgctggtg 4440 atctcaggac tttcctgt atcaatacca atcacggcag cagcatgta cctgtgggaa 4500 gtgaagaaac aacggggccgg agtattgtgg gatgttcctt caccccacc catgggaaag 4560 gctgaactgg aagatggagc ctatagaatt aagcaaaag ggattctgg atattcccag 4620 atcggagccg gagtttacaa agaaggaaca ttccatacaa tgtggcatgt cacacgtggc 4680 gctgttctaa tgcataagg aagaggatt gaaccatcat gggcggacgt gaaagac 4740 ctaatcat aggaggagg ctggaagtta gagagaat gggaagg gaggaagtc 4800 caggtattgg cactggagcc tggaaaaat ccaagagccg tccaacgaa acctggtctt 4860 ttcaaaacca acgccggaac ataggtgct gtatctctgg acttctcc tggaacgtca 4920 ggatctccaa ttatcgacaa aaaaggaaa gttgtgggtc tttagtaa tggtgttgtt 4980 acaaggagtg gagcatatgt gagtgctata gcccagactg aaaaagcat tgagacaac 5040 ccagagatcg aagatgacat ttccgaag agaagactga ccatcatgga cctccaccca 5100 ggagcgggaa agacgaagag ataccttccg gccatagtca gagaagctat aaacggggt 5160 ttgagaacat taatcttggc ccccactga gttgtggcag ctgaatgga ggaagccctt 5220 agaggacttc caataagata ccagacccca gccatcagag ctgtgcacac cggggcgggag 5280 attgtggacc taatgtgtca tgccacattt accatgaggc tgctatcacc attagagtg 5340 ccaaactaca acctgattat catggacgaa gcccattca cagacccagc aagtatagca 5400 gctagaggat acatctcaac tcgagtggag atgggtgagg cagctgggat ttttatgaca 5460 gccactcccc cgggaagcag agacccattt cctcagagca atgcaccaat catagatgaa 5520 gaaagagaaa tccctgaacg ctcgtggaat tccggacatg aatgggtcac ggattttaaa 5580 gggaagactg ttggttcgt tccaagtata aaagcaggaa atgatatagc agcttgcctg 5640 aggaaaaatg gaagaaagt gatcaactc agtaggaga cctttgattc tgagtatgtc 5700 aagactagaa ccaatgatg ggactcgtg gttacaactg acatttcaga aatgggtgcc 5760 aatttcagg ctgagaggggt tatagacccc agacgctgca tgaaccagt catactaca 5820 gatggtgaag agcggggtgat tctggcagga cctatgccag tgacccactc tagtgcagca 5880 aaagaagg ggaatagg aaaaatcca aaaatgaga atgaccagta catatacatg 5940 ggggaacctc tggaaatga tgagactgt gcacactgga aagaagcta atgctccta 6000 gataacatca acacgccaga aggaatcatt cctagcatgt tcgaaccaga gcgtgaaaag 6060 6120 ttaatgagaa gagagacct accagtctgg ttggcctaca gagtggcagc tgaaggcatc 6180 6240 aacgtggaag ttgaaatctg gacaaaagaa ggggaaagga agaattgaa acccagatgg 6300 ttggatgcta ggatctattc tgacccactg gcgctaaag aatttaagga atttgcagcc 6360 ggaagaaagt ctctgaccct gaacctaatc aagaaatgg gtaggctccc aaccttcatg 6420 actcagaagg caagacgc actggacaac ttagcagtgc tgcacacgg tgaggcaggt 6480 ggaagggcgt aaaccatgc tctcagtgaa ctgccggaga ccctgggagac attgctttta 6540 ctgacacttc tggctacagt cacgggagg atctttttat tcttgatgag cgcaaggggc 6600 atagggaga tgaccctggg aatgtgctgc ataatcacgg ctagcatcct cctatggtac 6660 gcaacaatac agccacactg gatagcagct tcaataatac tggagttttt tctcatagtt 6720 6780 gtcatagcca tcctcacagt ggtggccgca accatggcaa acgagatggg tttcctagaa 6840 aaaacgaaga aagatctcgg attgggaagc attgcaaccc agcaacccga gagcaacatc 6900 ctggacatag atctacgtcc tgcatcagca tggacgctgt atgccgtggc cacaacattt 6960 gttacaccaa tgttgagaca tagcattgaa aattcctcag tgaatgtgtc cctaacagct 7020 atagccaacc aagccacagt gttaatgggt ctcgggaaag gatggccatt gtcaaagatg 7080 gacatcggag ttccccttct cgccattgga tgctactcac aagtcaaccc cataactctc 7140 acagcagctc ttttcttatt ggtagcacat tatgccatca tagggccagg actccaagca 7200 aaagcaacca gagaagctca gaaaagagca gcggcgggca tcatgaaaaa cccaactgtc 7260 gatggaataa cagtgattga cctagatcca ataccttatg atccaaagtt tgaaaagcag 7320 ttgggacaag taatgctcct agtcctctgc gtgactcaag tattgatgat gaggactaca 7380 tgggctctgt gtgaggcttt aaccttagct accgggccca tctccacatt gtgggaagga 7440 aatccaggga ggttttggaa cactaccatt gcggtgtcaa tggctaacat ttttagaggg 7500 agttactttgg ccggagctgg acttctcttt tctattg agacacac cacacaga 7560 agggaactg gcaacatagg agagacgctt ggagagaat ggaaagccg attgaacgcg 7620 ttgggaaaaa gtgaattcca gatctacaag aaagtggaa tccaggagt ggatagaacc 7680 ttagcaaag aaggcattaa agaggagaa acggaccatc acgctgtgtc gcgaggctca 7740 gcaaaactga gatggttcgt tgagagaac atggtcacac cagaagggaa agtagtggac 7800 ctcggttgtg gcagaggagg ctggtcatac tattgtggag gactaagaa tgtaagagaa 7860 gtcaaggcc taacaaagg aggaccagga caccagaac ccatccccat gtcacatat 7920 gggtggaatc tagtgcgtct tcaagtgga gttgacgtttt tctcatccc gccagaaaag 7980 tgtgacacat tattgtgtga cataggggag tcatcaccaa atcccacagt ggaagcagga 8040 cgaacactca gagtccttaa cttagtagaa aattggttga acaacac tcaatttgc 8100 aaaggttc tcacccata tatgccctca gtcatagaaa aaatggaagc actacaagg 8160 aaatatggag gagccttagt gaggaatcca ctctcacgaa actccacaca tgagatgtac 8220 tgggtatcca atgcttccgg gaacatagtg tcatcagtga acatgatttc aaggatgttg 8280 atcacagat ttacaatgag atacagaaa gccacttacg agccgatgt tgacctcgga 8340 agcggaaccc gtaacatcgg gattgaagt gagatacca acctagatat aattgggaaa 8400 agaatagaaa aaaayagca agagcatgaa acatcatggc actatgacca agaccaccca 8460 tacaaaacgt gggcatacca tggtagctat gaacaaaac agactggatc agcatcatcc 8520 atggtcaacg gagtggtcag gctgctgaca aaaccttggg acgtcgtccc catggtgaca 8580 cagatggcaa tgacagacac gactccattt ggacaacagc gcgtttta agagaaagtg 8640 cccaaaacc gaaaaggc ahaaaaaaaaaaaaaaaaaaaaagggg 8700 tggctttgga agaattagg gagaaaaag acacccagga tgtgcaccag agaagaattc 8760 acaagaaagg tgagagcaa tgcagccttg ggggccatat tcactga gaacaagtgg 8820 aagtcggcac gtgaggctgt tgagatagt aggttttggg agctggttga caggaaagg 8880 aatctccatc ttgaaggaaa gtgtgaaaca tgtgtgtaca acatgatgggg aaaagagag 8940 aagaagctag gggaattcgg caagcaaa ggcagcagag ccatatggta catgtggctt 9000 ggagcacgct tcttagagtt tgaagcccta ggattcttta atgagatca ctggttctcc 9060 agagagaact ccctgagtgg agtgaagga gaagggctgc acagctagg ttacattcta 9120 agagacgtga gcaagaaaga gggaggagca atgtatgccg atgacaccgc aggatgggat 9180 acaaaaca cattagaga cctaaaaaat gagaatgg taacaacca catggaagga 9240 gaacacaga aactagccga ggccattttc aaactaacgt accaaaaaa ggtggtgcgt 9300 gtgcagac windstorm aggcacagta multiplying tatcgagag shaking 9360 ggtagtggac aagttggcac ctggactc atactttca ccaatatgga agcccacta 9420 atcagacaga tggaggga aggagtcttt aaagcattc agcacctaac atcacagaa 9480 gaaatcgctg tgcaaactg gttagcaaga gtggggcgcg aaaggttatc aagaatggcc 9540 atcagtggag atgattgtgt tgtgaaacct ttagatgaca ggttcgcaag cgctttaaca 9600 gctctaaatg acatgggaaa gattaggaaa gatacaac atgggaacc ttcaagagga 9660 tggaatgatt ggacacaagt gcccttctgt tcacaccatt tccatgagtt aatcatgaaa 9720 gacggtcgcg tactcgttgt tccatgtaga aaccaagatg aactgattgg cagagcccga 9780 atctcccaag gagcagggtg gtctttgcgg gagacggcct gtttggggaa gtcttacgcc 9840 caaatgtgga gcttgatgta cttccacaga cgcgacctca ggctggcggc aaatgctatt 9900 tgctcggcag taccatcaca ttgggttcca acaagtcgaa caacctggtc catacatgct 9960 aaacatgaat ggatgacaac ggaagacatg ctgacagtct ggaacagggt gtggattcaa 10020 gaaaacccat ggatggaaga caaaactcca gtggaatcat gggaggaaat cccatacttg 10080 gggaaaagag aagaccaatg gtgcggctca ttgattgggt taacaagcag ggccacctgg 10140 gcaaagaaca tccaagcagc aataaatcaa gttagatccc ttataggcaa tgaagaatac 10200 acagattaca tgccatccat gaaaagattc agaagagaag aggaagaagc aggagttctg 10260 tggtagaaag caaaactaac atgaaacaag gctagaagtc aggtcggatt aagccatagt 10320 acggaaaaaa ctatgctacc tgtgagcccc gtccaaggac gttaaaagaa gtcaggccat 10380 cataaatgcc atagcttgag taaactatgc agcctgtagc tccacctgag aaggtgtaaa 10440 aaatccggga ggccacaaac catggaagct gtacgcatgg cgtagtggac tagcggttag 10500 aggagacccc tcccttacaa atcgcagcaa caatgggggc ccaaggcgag atgaagctgt 10560 agtctcgctg gaaggactag aggttagagg agaccccccc gaaacaaaaa acagcatatt 10620 gacgctggga aagaccagag atcctgctgt ctcctcagca tcattccagg cacagaacgc 10680 cagaaaatgg aatggtgctg ttgaatcaac aggttct 10717 <210>18 <211>3389 <212>PRT <213>Artificial sequence <220> <223>Dengue virus serotype 3, pre-major <400>18 Met Asn Asn Gln Arg Lys Lys Ala Lys Asn Thr Pro Phe Asn Met Leu 1 5 10 15 Lys Arg Glu Arg Asn Arg Val Ser Thr Val Gln Gln Leu Thr Lys Arg 20 25 30[[ID=D3]] Phe Ser Leu Gly Met Leu Gln Gly Arg Gly Pro Leu Lys Leu Phe Met 35 40 45 Ala Leu Val Ala Phe Leu Arg Phe Leu Thr Ile Pro Pro Thr Ala Gly[[ID=D9]] 50 55 60 Ile Leu Lys Arg Trp Gly Thr Ile Lys Lys Ser Lys Ala Ile Asn Val 65 70 75 80 Leu Arg Gly Phe Arg Lys Glu Ile Gly Arg Met Leu Asn Ile Leu Asn 85 90 95 Arg Arg Arg Arg Ser Ala Gly Met Ile Ile Met Leu Ile Pro Thr Val 100 105 110 Met Ala Phe His Leu Thr Thr Arg Asp Gly Glu Pro Arg Met Ile Val 115 120 125 Gly Lys Asn Glu Arg Gly Lys Ser Leu Leu Phe Lys Thr Ala Ser Gly 130 135 140 Ile Asn Met Cys Thr Leu Ile Ala Met Asp Leu Gly Glu Met Cys Asp 145 150 155 160 Asp Thr Val Thr Tyr Lys Cys Pro His Ile Thr Glu Val Glu Pro Glu 165 170 175 Asp Ile Asp Cys Trp Cys Asn Leu Thr Ser Thr Trp Val Thr Tyr Gly 180 185 190 Thr Cys Asn Gln Ala Gly Glu His Arg Arg Asp Lys Arg Ser Val Ala 195 200 205 Leu Ala Pro His Val Gly Met Gly Leu Asp Thr Arg Thr Gln Thr Trp 210 215 220 Met Ser Ala Glu Gly Ala Trp Arg Gln Val Glu Lys Val Glu Thr Trp 225 230 235 240 Ala Leu Arg His Pro Gly Phe Thr Ile Leu Ala Leu Phe Leu Ala His 245 250 255 Tyr Ile Gly Thr Ser Leu Thr Gln Lys Val Val Ile Phe Ile Leu Leu 260 265 270 Met Leu Val Thr Pro Ser Met Thr Met Arg Cys Val Gly Val Gly Asn 275 280 285 Arg Asp Phe Val Glu Gly Leu Ser Gly Ala Thr Trp Val Asp Val Val 290 295 300 Leu Glu His Gly Gly Cys Val Thr Thr Met Ala Lys Asn Lys Pro Thr 305 310 315 320 Leu Asp Ile Glu Leu Gln Lys Thr Glu Ala Thr Gln Leu Ala Thr Leu 325 330 335 Arg Lys Leu Cys Ile Glu Gly Lys Ile Thr Asn Ile Thr Thr Asp Ser 340 345 350 Arg Cys Pro Thr Gln Gly Glu Ala Ile Leu Pro Glu Glu Gln Asp Gln 355 360 365 Asn Tyr Val Cys Lys His Thr Tyr Val Asp Arg Gly Trp Gly Asn Gly 370 375 380 Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Gln 385 390 395 400 Cys Leu Glu Ser Ile Glu Gly Lys Val Val Gln His Glu Asn Leu Lys 405 410 415 Tyr Thr Val Ile Ile Thr Val His Thr Gly Asp Gln His Gln Val Gly 420 425 430 Asn Glu Thr Gln Gly Val Thr Ala Glu Ile Thr Pro Gln Ala Ser Thr 435 440 445 Ala Glu Ala Ile Leu Pro Glu Tyr Gly Thr Leu Gly Leu Glu Cys Ser 450 455 460 Pro Arg Thr Gly Leu Asp Phe Asn Glu Met Ile Ser Leu Thr Met Lys 465 470 475 480 Asn Lys Ala Trp Met Val His Arg Gln Trp Phe Phe Asp Leu Pro Leu 485 490 495 Pro Trp Thr Ser Gly Ala Ser Ala Glu Thr Pro Thr Trp Asn Arg Lys 500 505 510 Glu Leu Leu Val Thr Phe Lys Asn Ala His Ala Lys Lys Gln Glu Val 515 520 525 Val Val Leu Gly Ser Gln Glu Gly Ala Met His Thr Ala Leu Thr Gly 530 535 540 Ala Thr Glu Ile Gln Thr Ser Gly Gly Thr Ser Ile Phe Ala Gly His 545 550 555 560 Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Glu Leu Lys Gly Met Ser 565 570 575 Tyr Ala Met Cys Leu Ser Ser Phe Val Leu Lys Lys Glu Val Ser Glu 580 585 590 Thr Gln His Gly Thr Ile Leu Ile Lys Val Glu Tyr Lys Gly Glu Asp 595 600 605 Ala Pro Cys Lys Ile Pro Phe Ser Thr Glu Asp Gly Gln Gly Lys Ala 610 615 620 Leu Asn Gly Arg Leu Ile Thr Ala Asn Pro Val Val Thr Lys Lys Glu 625 630 635 640 Glu Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser Asn Ile 645 650 655 Val Ile Gly Ile Gly Asp Lys Ala Leu Lys Ile Asn Trp Tyr Lys Lys 660 665 670 Gly Ser Ser Ile Gly Lys Met Phe Glu Ala Thr Ala Arg Gly Ala Arg 675 680 685 Arg Met Ala Ile Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly 690 695 700 Gly Val Leu Asn Ser Leu Gly Lys Met Val His Gln Ile Phe Gly Ser 705 710 715 720 Ala Tyr Thr Ala Leu Phe Gly Gly Val Ser Trp Met Met Lys Ile Gly 725 730 735 Ile Gly Val Leu Leu Thr Trp Ile Gly Leu Asn Ser Lys Asn Thr Ser 740 745 750 Met Ser Phe Ser Cys Ile Ala Ala Gly Ile Val Thr Leu Tyr Leu Gly 755 760 765 Val Met Val Gln Ala Asp Ser Gly Cys Val Val Ser Trp Lys Asn Lys 770 775 780 Glu Leu Lys Cys Gly Ser Gly Ile Phe Ile Thr Asp Asn Val His Thr 785 790 795 800 Trp Thr Glu Gln Tyr Lys Phe Gln Pro Glu Ser Pro Ser Lys Leu Ala 805 810 815 Ser Ala Ile Gln Lys Ala His Glu Glu Asp Ile Cys Gly Ile Arg Ser 820 825 830 Val Thr Arg Leu Glu Asn Leu Met Trp Lys Gln Ile Thr Pro Glu Leu 835 840 845 Asn His Ile Leu Ser Glu Asn Glu Val Lys Leu Thr Ile Met Thr Gly 850 855 860 Asp Ile Lys Gly Ile Met Gln Ala Gly Lys Arg Ser Leu Arg Pro Gln 865 870 875 880 Pro Thr Glu Leu Lys Tyr Ser Trp Lys Thr Trp Gly Lys Ala Lys Met 885 890 895 Leu Ser Thr Glu Ser His Asn Gln Thr Phe Leu Ile Asp Gly Pro Glu 900 905 910 Thr Ala Glu Cys Pro Asn Thr Asn Arg Ala Trp Asn Ser Leu Glu Val 915 920 925 Glu Asp Tyr Gly Phe Gly Val Phe Thr Thr Asn Ile Trp Leu Lys Leu 930 935 940 Lys Glu Lys Gln Asp Val Phe Cys Asp Ser Lys Leu Met Ser Ala Ala 945 950 955 960 Ile Lys Asp Asn Arg Ala Val His Ala Asp Met Gly Tyr Trp Ile Glu 965 970 975 Ser Ala Leu Asn Asp Thr Trp Lys Ile Glu Lys Ala Ser Phe Ile Glu 980 985 990 Val Lys Asn Cys His Trp Pro Lys Ser His Thr Leu Trp Ser Asn Gly 995 1000 1005 Val Leu Glu Ser Glu Met Ile Ile Pro Lys Asn Leu Ala Gly Pro 1010 1015 1020 Val Ser Gln His Asn Tyr Arg Pro Gly Tyr His Thr Gln Ile Thr 1025 1030 1035 Gly Pro Trp His Leu Gly Lys Leu Glu Met Asp Phe Asp Phe Cys 1040 1045 1050 Asp Gly Thr Thr Val Val Val Thr Glu Asp Cys Gly Asn Arg Gly 1055 1060 1065 Pro Ser Leu Arg Thr Thr Thr Ala Ser Gly Lys Leu Ile Thr Glu 1070 1075 1080 Trp Cys Cys Arg Ser Cys Thr Leu Pro Pro Leu Arg Tyr Arg Gly 1085 1090 1095 Glu Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Leu Lys Glu 1100 1105 1110 Lys Glu Glu Asn Leu Val Asn Ser Leu Val Thr Ala Gly His Gly 1115 1120 1125 Gln Val Asp Asn Phe Ser Leu Gly Val Leu Gly Met Ala Leu Phe 1130 1135 1140 Leu Glu Glu Met Leu Arg Thr Arg Val Gly Thr Lys His Ala Ile 1145 1150 1155 Leu Leu Val Ala Val Ser Phe Val Thr Leu Ile Thr Gly Asn Met 1160 1165 1170 Ser Phe Arg Asp Leu Gly Arg Val Met Val Met Val Gly Ala Thr 1175 1180 1185 Met Thr Asp Asp Ile Gly Met Gly Val Thr Tyr Leu Ala Leu Leu 1190 1195 1200 Ala Ala Phe Lys Val Arg Pro Thr Phe Ala Ala Gly Leu Leu Leu 1205 1210 1215 Arg Lys Leu Thr Ser Lys Glu Leu Met Met Thr Thr Ile Gly Ile 1220 1225 1230 Val Leu Leu Ser Gln Ser Thr Ile Pro Glu Thr Ile Leu Glu Leu 1235 1240 1245 Thr Asp Ala Leu Ala Leu Gly Met Met Val Leu Lys Met Val Arg 1250 1255 1260 Asn Met Glu Lys Tyr Gln Leu Ala Val Thr Ile Met Ala Ile Leu 1265 1270 1275 Cys Val Pro Asn Ala Val Ile Leu Gln Asn Ala Trp Lys Val Ser 1280 1285 1290 Cys Thr Ile Leu Ala Val Val Ser Val Ser Pro Leu Phe Leu Thr 1295 1300 1305 Ser Ser Gln Gln Lys Thr Asp Trp Ile Pro Leu Ala Leu Thr Ile 1310 1315 1320 Lys Gly Leu Asn Pro Thr Ala Ile Phe Leu Thr Thr Leu Ser Arg 1325 1330 1335 Thr Ser Lys Lys Arg Ser Trp Pro Leu Asn Glu Ala Ile Met Ala 1340 1345 1350 Val Gly Met Val Ser Ile Leu Ala Ser Ser Leu Leu Lys Asn Asp 1355 1360 1365 Ile Pro Met Thr Gly Pro Leu Val Ala Gly Gly Leu Leu Thr Val 1370 1375 1380 Cys Tyr Val Leu Thr Gly Arg Ser Ala Asp Leu Glu Leu Glu Arg 1385 1390 1395 Ala Ala Asp Val Lys Trp Glu Asp Gln Ala Glu Ile Ser Gly Ser 1400 1405 1410 Ser Pro Ile Leu Ser Ile Thr Ile Ser Glu Asp Gly Ser Met Ser 1415 1420 1425 Ile Lys Asn Glu Glu Glu Glu Gln Thr Leu Thr Ile Leu Ile Arg 1430 1435 1440 Thr Gly Leu Leu Val Ile Ser Gly Leu Phe Pro Val Ser Ile Pro 1445 1450 1455 Ile Thr Ala Ala Ala Trp Tyr Leu Trp Glu Val Lys Lys Gln Arg 1460 1465 1470 Ala Gly Val Leu Trp Asp Val Pro Ser Pro Pro Pro Met Gly Lys 1475 1480 1485 Ala Glu Leu Glu Asp Gly Ala Tyr Arg Ile Lys Gln Lys Gly Ile 1490 1495 1500 Leu Gly Tyr Ser Gln Ile Gly Ala Gly Val Tyr Lys Glu Gly Thr 1505 1510 1515 Phe His Thr Met Trp His Val Thr Arg Gly Ala Val Leu Met His 1520 1525 1530 Lys Gly Lys Arg Ile Glu Pro Ser Trp Ala Asp Val Lys Lys Asp 1535 1540 1545 Leu Ile Ser Tyr Gly Gly Gly Trp Lys Leu Glu Gly Glu Trp Lys 1550 1555 1560 Glu Gly Glu Glu Val Gln Val Leu Ala Leu Glu Pro Gly Lys Asn 1565 1570 1575 Pro Arg Ala Val Gln Thr Lys Pro Gly Leu Phe Lys Thr Asn Ala 1580 1585 1590 Gly Thr Ile Gly Ala Val Ser Leu Asp Phe Ser Pro Gly Thr Ser 1595 1600 1605 Gly Ser Pro Ile Ile Asp Lys Lys Gly Lys Val Val Gly Leu Tyr 1610 1615 1620 Gly Asn Gly Val Val Thr Arg Ser Gly Ala Tyr Val Ser Ala Ile 1625 1630 1635 Ala Gln Thr Glu Lys Ser Ile Glu Asp Asn Pro Glu Ile Glu Asp 1640 1645 1650 Asp Ile Phe Arg Lys Arg Arg Leu Thr Ile Met Asp Leu His Pro 1655 1660 1665 Gly Ala Gly Lys Thr Lys Arg Tyr Leu Pro Ala Ile Val Arg Glu 1670 1675 1680 Ala Ile Lys Arg Gly Leu Arg Thr Leu Ile Leu Ala Pro Thr Arg 1685 1690 1695 Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu Pro Ile 1700 1705 1710 Arg Tyr Gln Thr Pro Ala Ile Arg Ala Val His Thr Gly Arg Glu 1715 1720 1725 Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Met Arg Leu Leu 1730 1735 1740 Ser Pro Val Arg Val Pro Asn Tyr Asn Leu Ile Ile Met Asp Glu 1745 1750 1755 Ala His Phe Thr Asp Pro Ala Ser Ile Ala Ala Arg Gly Tyr Ile 1760 1765 1770 Ser Thr Arg Val Glu Met Gly Glu Ala Ala Gly Ile Phe Met Thr 1775 1780 1785 Ala Thr Pro Pro Gly Ser Arg Asp Pro Phe Pro Gln Ser Asn Ala 1790 1795 1800 Pro Ile Ile Asp Glu Glu Arg Glu Ile Pro Glu Arg Ser Trp Asn 1805 1810 1815 Ser Gly His Glu Trp Val Thr Asp Phe Lys Gly Lys Thr Val Trp 1820 1825 1830 Phe Val Pro Ser Ile Lys Ala Gly Asn Asp Ile Ala Ala Cys Leu 1835 1840 1845 Arg Lys Asn Gly Lys Lys Val Ile Gln Leu Ser Arg Lys Thr Phe 1850 1855 1860 Asp Ser Glu Tyr Val Lys Thr Arg Thr Asn Asp Trp Asp Phe Val 1865 1870 1875 Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys Ala Glu 1880 1885 1890 Arg Val Ile Asp Pro Arg Arg Cys Met Lys Pro Val Ile Leu Thr 1895 1900 1905 Asp Gly Glu Glu Arg Val Ile Leu Ala Gly Pro Met Pro Val Thr 1910 1915 1920 His Ser Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg Asn Pro 1925 1930 1935 Lys Asn Glu Asn Asp Gln Tyr Ile Tyr Met Gly Glu Pro Leu Glu 1940 1945 1950 Asn Asp Glu Asp Cys Ala His Trp Lys Glu Ala Lys Met Leu Leu 1955 1960 1965 Asp Asn Ile Asn Thr Pro Glu Gly Ile Ile Pro Ser Met Phe Glu 1970 1975 1980 Pro Glu Arg Glu Lys Val Asp Ala Ile Asp Gly Glu Tyr Arg Leu 1985 1990 1995 Arg Gly Glu Ala Arg Lys Thr Phe Val Asp Leu Met Arg Arg Gly 2000 2005 2010 Asp Leu Pro Val Trp Leu Ala Tyr Arg Val Ala Ala Glu Gly Ile 2015 2020 2025 Asn Tyr Ala Asp Arg Arg Trp Cys Phe Asp Gly Val Lys Asn Asn 2030 2035 2040 Gln Ile Leu Glu Glu Asn Val Glu Val Glu Ile Trp Thr Lys Glu 2045 2050 2055 Gly Glu Arg Lys Lys Leu Lys Pro Arg Trp Leu Asp Ala Arg Ile 2060 2065 2070 Tyr Ser Asp Pro Leu Ala Leu Lys Glu Phe Lys Glu Phe Ala Ala 2075 2080 2085 Gly Arg Lys Ser Leu Thr Leu Asn Leu Ile Thr Glu Met Gly Arg 2090 2095 2100 Leu Pro Thr Phe Met Thr Gln Lys Ala Arg Asp Ala Leu Asp Asn 2105 2110 2115 Leu Ala Val Leu His Thr Ala Glu Ala Gly Gly Arg Ala Tyr Asn 2120 2125 2130 His Ala Leu Ser Glu Leu Pro Glu Thr Leu Glu Thr Leu Leu Leu 2135 2140 2145 Leu Thr Leu Leu Ala Thr Val Thr Gly Gly Ile Phe Leu Phe Leu 2150 2155 2160 Met Ser Ala Arg Gly Ile Gly Lys Met Thr Leu Gly Met Cys Cys 2165 2170 2175 Ile Ile Thr Ala Ser Ile Leu Leu Trp Tyr Ala Gln Ile Gln Pro 2180 2185 2190 His Trp Ile Ala Ala Ser Ile Ile Leu Glu Phe Phe Leu Ile Val 2195 2200 2205 Leu Leu Ile Pro Glu Pro Glu Lys Gln Arg Thr Pro Gln Asp Asn 2210 2215 2220 Gln Leu Thr Tyr Val Val Ile Ala Ile Leu Thr Val ValAla Ala 2225 2230 2235 Thr Met Ala Asn Glu Met Gly Phe Leu Glu Lys Thr Lys Lys Asp 2240 2245 2250 Leu Gly Leu Gly Ser Ile Ala Thr Gln Gln Pro Glu Ser Asn Ile 2255 2260 2265 Leu Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Thr Leu Tyr Ala 2270 2275 2280 Val Ala Thr Thr Phe Val Thr Pro Met Leu Arg His Ser Ile Glu 2285 2290 2295 Asn Ser Ser Val Asn Val Ser Leu Thr Ala Ile Ala Asn Gln Ala 2300 2305 2310 Thr Val Leu Met Gly Leu Gly Lys Gly Trp Pro Leu Ser Lys Met 2315 2320 2325 Asp Ile Gly Val Pro Leu Leu Ala Ile Gly Cys Tyr Ser Gln Val 2330 2335 2340 Asn Pro Ile Thr Leu Thr Ala Ala Leu Phe Leu Leu Val Ala His 2345 2350 2355 Tyr Ala Ile Ile Gly Pro Gly Leu Gln Ala Lys Ala Thr Arg Glu 2360 2365 2370 Ala Gln Lys Arg Ala Ala Ala Gly Ile Met Lys Asn Pro Thr Val 2375 2380 2385 Asp Gly Ile Thr Val Ile Asp Leu Asp Pro Ile Pro Tyr Asp Pro 2390 2395 2400 Lys Phe Glu Lys Gln Leu Gly Gln Val Met Leu Leu Val Leu Cys 2405 2410 2415 Val Thr Gln Val Leu Met Met Arg Thr Thr Trp Ala Leu Cys Glu 2420 2425 2430 Ala Leu Thr Leu Ala Thr Gly Pro Ile Ser Thr Leu Trp Glu Gly 2435 2440 2445 Asn Pro Gly Arg Phe Trp Asn Thr Thr Ile Ala Val Ser Met Ala 2450 2455 2460 Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Gly Leu Leu Phe 2465 2470 2475 Ser Ile Met Lys Asn Thr Thr Asn Thr Arg Arg Gly Thr Gly Asn 2480 2485 2490 Ile Gly Glu Thr Leu Gly Glu Lys Trp Lys Ser Arg Leu Asn Ala 2495 2500 2505 Leu Gly Lys Ser Glu Phe Gln Ile Tyr Lys Lys Ser Gly Ile Gln 2510 2515 2520 Glu Val Asp Arg Thr Leu Ala Lys Glu Gly Ile Lys Arg Gly Glu 2525 2530 2535 Thr Asp His His Ala Val Ser Arg Gly Ser Ala Lys Leu Arg Trp 2540 2545 2550 Phe Val Glu Arg Asn Met Val Thr Pro Glu Gly Lys Val Val Asp 2555 2560 2565 Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Cys Gly Gly Leu 2570 2575 2580 Lys Asn Val Arg Glu Val Lys Gly Leu Thr Lys Gly Gly Pro Gly 2585 2590 2595 His Glu Glu Pro Ile Pro Met Ser Thr Tyr Gly Trp Asn Leu Val 2600 2605 2610 Arg Leu Gln Ser Gly Val Asp Val Phe Phe Ile Pro Pro Glu Lys 2615 2620 2625 Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Pro Asn Pro 2630 2635 2640 Thr Val Glu Ala Gly Arg Thr Leu Arg Val Leu Asn Leu Val Glu 2645 2650 2655 Asn Trp Leu Asn Asn Asn Thr Gln Phe Cys Ile Lys Val Leu Asn 2660 2665 2670 Pro Tyr Met Pro Ser Val Ile Glu Lys Met Glu Ala Leu Gln Arg 2675 2680 2685 Lys Tyr Gly Gly Ala Leu Val Arg Asn Pro Leu Ser Arg Asn Ser 2690 2695 2700 Thr His Glu Met Tyr Trp Val Ser Asn Ala Ser Gly Asn Ile Val 2705 2710 2715 Ser Se...

Claims

1. Use of a tetravalent vaccine formulation in the preparation of a pharmaceutical composition for a treatment method for an individual infected with dengue virus, the tetravalent vaccine formulation consisting of all four dengue virus serotypes, including live attenuated dengue-2 virus, dengue-2 / dengue-1 chimera, dengue-2 / dengue-3 chimera and dengue-2 / dengue-4 chimera, the method comprising: administering the pharmaceutical composition subcutaneously to the individual, wherein administering the pharmaceutical composition consists of administering a first dose of the pharmaceutical composition on day 0 and administering a second dose of the same pharmaceutical composition against dengue virus within 3 months or 90 days of the first administration, wherein the pharmaceutical composition induces an immune response against dengue virus in the individual; wherein the individual is 2 to 17 years old; And wherein the amino acid sequence encoding the live attenuated dengue-2 virus is shown as SEQ ID NO:12, the amino acid sequence encoding the dengue-2 / dengue-1 chimera is shown as SEQ ID NO:4, the amino acid sequence encoding the dengue-2 / dengue-3 chimera is shown as SEQ ID NO:20, and the amino acid sequence encoding the dengue-2 / dengue-4 chimera is shown as SEQ ID NO:

28.

2. Use of a tetravalent vaccine formulation in the preparation of a pharmaceutical composition for a treatment method for an individual infected with a dengue virus, the tetravalent vaccine formulation consisting of all four dengue virus serotypes, including live attenuated dengue-2 virus, dengue-2 / dengue-1 chimera, dengue-2 / dengue-3 chimera and dengue-2 / dengue-4 chimera, the method comprising: administering the pharmaceutical composition subcutaneously to the individual, wherein administering the pharmaceutical composition consists of administering a first dose of the pharmaceutical composition on day 0 and administering a second dose of the same pharmaceutical composition against dengue virus within 3 months or 90 days of the first administration, wherein the pharmaceutical composition induces an immune response against dengue virus in the individual; wherein the individual is between 1 and 11 years old; And wherein the amino acid sequence encoding the live attenuated dengue-2 virus is shown as SEQ ID NO:12, the amino acid sequence encoding the dengue-2 / dengue-1 chimera is shown as SEQ ID NO:4, the amino acid sequence encoding the dengue-2 / dengue-3 chimera is shown as SEQ ID NO:20, and the amino acid sequence encoding the dengue-2 / dengue-4 chimera is shown as SEQ ID NO:

28.

3. The use according to claim 1 or 2, wherein the concentration ratio of the dengue-2 / dengue-1 chimera to the live attenuated dengue-2 virus to the dengue-2 / dengue-3 chimera to the dengue-2 / dengue-4 chimera is 4:4:5:5 calculated in terms of plaque forming units.

4. The use according to claim 1 or 2, wherein the concentration ratio of the dengue-2 / dengue-1 chimera to the live attenuated dengue-2 virus to the dengue-2 / dengue-3 chimera to the dengue-2 / dengue-4 chimera is 5:4:5:5 calculated in terms of plaque forming units.

5. The use according to claim 1 or 2, wherein the pharmaceutical composition further comprises a stabilizing buffer. The use according to claim 5 , wherein the stabilizing buffer comprises trehalose and albumin.

7. The method according to claim 6, wherein the stabilizing buffer further comprises poloxamer 407.

8. The use according to claim 1 or 2, wherein the individual is seronegative or non-immune to dengue virus before administration of the pharmaceutical composition.

9. The use according to claim 1 or 2, wherein the individual is seropositive for the dengue virus before administration of the pharmaceutical composition.

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