A kind of rice double haploid breeding method based on haploid induction line

Abstract

The invention provides a rice doubled haploid breeding method based on a haploid inducer and belongs to the technical field of agriculture. According to the method herein, a rice three-line maintainermaterial is hybridized with a rice material having a dominant red rice gene, a red rice maintainer carrying the dominant red rice gene are attained by screening, the red rice maintainer is subjectedto chemical directional induction, a mutant with a centromere mutant gene function is attained by screening, and the mutant is hybridized with a rice three-line sterile line to obtain a maintainer andsterile line carrying the red rice gene and centromere mutant gene; a hybrid F1 prepared according to breeding targets is hybridized with a red rice haploid induced sterile line before colorless-coathybrid seeds are retained; the colorless hybrid seeds are treated with colchicine to obtain a diploid material, namely, a novel line is formed. The rice doubled haploid breeding method based on the haploid inducer allows a homozygous gene to be attained quickly, helps overcome the defect segregative generations in traditional breeding are long, and is applicable to rice breeding.

Description

technical field

[0001] The invention relates to a rice double haploid breeding method based on a haploid induction system, which belongs to the field of agricultural technology. Background technique

[0002] Haploid breeding technology is to obtain purified double haploid (DH plants) by doubling the chromosome group of natural or artificially cultivated haploid plants. It is more and more popular among breeders for its fast, accurate and efficient characteristics At present, more than 250 crop species in the world have applied double haploid breeding, and more than 300 double haploid pure lines from cultivated species have been bred in 12 species. The core of traditional breeding technology is to fix favorable genes and make them homozygous through continuous selection. This process usually requires multiple generations of self-crossing or backcrossing. Lines need more than 6 generations, and the use of double haploid breeding method generally only needs 2 generations, whic...

Examples

Embodiment Construction

[0028] The present invention will be further described below in combination with specific embodiments and accompanying drawings.

[0029] A kind of rice double haploid breeding method based on haploid induction line, such as figure 1 shown, including the following steps:

[0030] 1. The maintainer line H353-B carrying multiple favorable genes was transformed into a red rice maintainer line.

[0031] In the early stage, our research group bred a three-line maintainer line H353-B carrying 3 excellent genes (amylose content gene Wx, aroma gene fgr, and rice blast resistance gene Pita), and bred its homokaryotic heterogeneous The sterile line H353-A was crossed to obtain highly sterile F1. Using a material H22 (male parent) carrying a dominant red seed coat gene RR to cross with H353-B (female parent), the seed coat of the F1 generation is red, and H353-B is used as the recurrent parent, and each backcross generation is selected The red seed coat individual was backcrossed with...

technical field

[0001] The invention relates to a rice double haploid breeding method based on a haploid induction system, which belongs to the field of agricultural technology. Background technique

[0002] Haploid breeding technology is to obtain purified double haploid (DH plants) by doubling the chromosome group of natural or artificially cultivated haploid plants. It is more and more popular among breeders for its fast, accurate and efficient characteristics At present, more than 250 crop species in the world have applied double haploid breeding, and more than 300 double haploid pure lines from cultivated species have been bred in 12 species. The core of traditional breeding technology is to fix favorable genes and make them homozygous through continuous selection. This process usually requires multiple generations of self-crossing or backcrossing. Lines need more than 6 generations, and the use of double haploid breeding method generally only needs 2 generations, whic...

Examples

Embodiment Construction

[0028] The present invention will be further described below in combination with specific embodiments and accompanying drawings.

[0029] A kind of rice double haploid breeding method based on haploid induction line, such as figure 1 shown, including the following steps:

[0030] 1. The maintainer line H353-B carrying multiple favorable genes was transformed into a red rice maintainer line.

[0031] In the early stage, our research group bred a three-line maintainer line H353-B carrying 3 excellent genes (amylose content gene Wx, aroma gene fgr, and rice blast resistance gene Pita), and bred its homokaryotic heterogeneous The sterile line H353-A was crossed to obtain highly sterile F1. Using a material H22 (male parent) carrying a dominant red seed coat gene RR to cross with H353-B (female parent), the seed coat of the F1 generation is red, and H353-B is used as the recurrent parent, and each backcross generation is selected The red seed coat individual was backcrossed with...

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