Press-type reaction tube

Abstract

The present invention discloses a push-type reaction tube, comprising a tube body and an upper cover, the tube body and the upper cover are sealed and connected as one through a connecting sleeve, the diaphragm on the annular limiting platform in the connecting sleeve separates the connecting sleeve into an upper connecting section and a lower connecting section, a limiting card platform matched with the annular limiting platform is provided on the outer side wall of the lower end of the upper cover, a film cutting structure is provided in the upper cover, and the film cutting structure is located above the diaphragm; a limiting structure is provided between the upper cover and the upper connecting section; a solid reagent is encapsulated in the upper cover, and a liquid reagent is encapsulated in the tube body. The present invention encapsulates the solid reagent and the liquid reagent in the upper cover and the tube body respectively, and separates them by a diaphragm. When in use, the upper cover is pressed down to puncture the diaphragm by cutting teeth, and the solid reagent enters the tube body from the upper cover and is fully mixed with the liquid reagent in the tube body, and there is no need to manually add amplification reagents multiple times, which simplifies the operation steps, shortens the reagent addition time, improves work efficiency, shortens the reagent exposure time, and reduces the probability of contamination.

Description

Technical Field

[0001] The invention relates to a PCR container, in particular to a press-type reaction tube. Background Art

[0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. With its high specificity, high sensitivity and high efficiency, it has had a huge impact in the field of life science research. PCR reaction tubes are a type of reaction container commonly used in PCR. The in vitro amplification of DNA is achieved by repeatedly raising and lowering the temperature of the reaction solution in the PCR reaction tube by a PCR thermal cycler. The PCR reaction tube includes a tube body and a tube cover. For example, CN106906130A discloses a high-transmittance flat-bottom PCR reaction tube, and CN201981185U discloses a high-speed PCR reaction tube. However, the existing PCR reaction tubes have the following problems: Since there are at least 5 reagents used in the DNA amplification reaction, the sample and multiple reagents need to be manually and accurately added to the PCR reaction tube in sequence before DNA amplification, which is time-consuming and cumbersome to operate, and the reagents in the tube body are exposed for a long time, which increases the probability of contamination of the sample in the tube body. Therefore, how to design a PCR reaction tube that can simplify the PCR reagent steps is a key problem that technicians in this field need to solve. Summary of the invention

[0003] The present invention aims to provide a push-type reaction tube encapsulating solid reagents and liquid reagents. When in use, a plurality of different reagents can be mixed by puncturing the diaphragm without repeatedly opening and closing the upper cover, which greatly reduces the probability of cross-contamination of samples.

[0004] To achieve the above object, the present invention adopts the following technical solutions:

[0005] The push-type reaction tube described in the present invention comprises a tube body and an upper cover, which are sealed and connected as a whole through a connecting sleeve, a diaphragm is arranged on the annular limit platform in the connecting sleeve, and the diaphragm separates the connecting sleeve into an upper connecting section for connecting the upper cover and a lower connecting section for connecting the tube body, a limit clamping platform matched with the annular limit platform is arranged on the outer wall of the lower end of the upper cover, a film cutting structure for puncturing the diaphragm is arranged in the upper cover, and the film cutting structure is located above the diaphragm; a limiting structure is arranged between the upper cover and the upper connecting section; a solid reagent is encapsulated in the upper cover, and a liquid reagent is encapsulated in the tube body.

[0006] The upper cover is sealably inserted in the upper connecting section, and the tube body is sealably inserted in the lower connecting section.

[0007] The limiting structure includes a pair of annular positioning grooves arranged at intervals on the inner side wall of the upper connecting section, a lower positioning strip matching the annular positioning groove is arranged on the outer side wall of the upper cover close to the limiting clamping platform, an upper positioning strip is arranged on the outer side wall of the upper cover above the lower positioning strip, the spacing between the upper positioning strip and the lower positioning strip is equal to the spacing between the two annular positioning grooves, and the lower positioning strip is clamped in the annular positioning groove located above.

[0008] The film cutting structure includes a film cutting knife extending vertically downward from the inner edge of the limiting clamping table and having an annular structure. The bottom of the film cutting knife includes a cutting part and a film pressing part. The cutting part is provided with a plurality of cutting teeth extending downward along its circumference. The length of the film pressing part is L Circumference of the bottom of the film cutting knife C Satisfies the following relationship: .

[0009] The tube body comprises a wide-mouth section and a thin tube section, and the wide-mouth section and the thin tube section are connected as a whole through a truncated cone section.

[0010] A clamping strip is arranged on the outer side wall of the wide-mouth section, and an annular fixing groove matched with the clamping strip is arranged in the lower connecting section.

[0011] The advantages of the present invention are that it overcomes the technical problems of high probability of contamination and long time consumption in traditional PCR reaction tubes due to the need to add reagents multiple times. The advantages of the present invention are specifically reflected in the following points:

[0012] (1) The present invention encapsulates solid reagents and liquid reagents in an upper cover and a tube body respectively, and separates them by a diaphragm. When in use, the tube body is removed, and the sample and diluent are added to the tube body. The tube body is then inserted into the connecting sleeve, and the upper cover is pressed down to pierce the diaphragm with the cutting teeth. The solid reagent enters the tube body from the upper cover and is fully mixed with the liquid reagent in the tube body. There is no need to manually add amplification reagents multiple times, which simplifies the operation steps, shortens the reagent addition time, improves work efficiency, greatly shortens the reagent exposure time, and reduces the chance of contamination.

[0013] (2) The limiting structure of the present invention comprises a pair of positioning grooves and upper and lower positioning strips. When not in use, the lower seal of the upper cover is inserted into the upper connecting section and the lower positioning strip is clamped into the upper positioning groove. This can not only prevent the diaphragm from being punctured by the cutting teeth, but also has a positioning function and ensures the sealing performance of the upper cover and the connecting sleeve. The clamping strip of the tube body is clamped into the annular positioning groove to ensure the sealing performance of the tube body and the connecting sleeve.

[0014] (3) The film cutting knife of the present invention is an annular structure. The bottom of the film cutting knife is composed of a cutting part and a film pressing part. When cutting, the cutting teeth pierce the diaphragm while ensuring that the diaphragm corresponding to the film pressing part is connected to the annular limit platform to prevent the diaphragm from falling into the tube body below and affecting the amplification effect.

[0015] (4) The tube body of the present invention is composed of a wide-mouth section, a truncated cone section and a thin tube section. The wide-mouth section at the top can ensure that the solid reagent falls smoothly into the truncated cone section or the thin tube section. The thin tube section increases the contact area between the sample and the reagent, thereby improving the reaction rate between the sample and the reagent. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural schematic diagram of the present invention.

[0017] Figure 2 It is a schematic diagram of the structure after the diaphragm of the present invention is punctured.

[0018] Figure 3 yes Figure 2 Connection diagram of the middle connecting sleeve and the upper cover.

[0019] Figure 4 yes Figure 2 Connection diagram between the middle connecting sleeve and the pipe body.

[0020] Figure 5 It is a structural schematic diagram of the upper cover of the present invention. DETAILED DESCRIPTION

[0021] like Figure 1 As shown, the push-type reaction tube described in the present invention includes a tube body and an upper cover 1, and the tube body and the upper cover 1 are sealed and connected as a whole through a connecting sleeve. A diaphragm 2.2 made of tin foil is arranged on the annular limiting platform 2.1 in the connecting sleeve, and the diaphragm 2.2 separates the connecting sleeve into an upper connecting section 2.3 for connecting the upper cover 1 and a lower connecting section 2.4 for connecting the tube body, that is, the diaphragm 2.2 separates the upper cover 1 and the tube body into two sealed cavities; a limiting card platform 3 matched with the annular limiting platform 2.1 is arranged on the outer side wall of the lower end of the upper cover 1, and a film cutting structure for piercing the diaphragm 2.2 is arranged in the upper cover 1, and the film cutting structure is located above the diaphragm 2.2; a limiting structure is arranged between the upper cover 1 and the upper connecting section 2.3; solid reagents such as freeze-dried beads and wax beads are encapsulated in the upper cover 1, and the liquid reagent encapsulated in the tube body is a mixed solution of multiple liquid reagents for amplification.

[0022] like Figure 1 , 3As shown, the upper cover 1 is sealed and inserted in the upper connecting section 2.3, and the limiting structure includes a pair of annular positioning grooves 4.1 arranged at intervals on the inner wall of the upper connecting section 2.3. A lower positioning strip 4.2 matching the annular positioning groove 4.1 is arranged on the outer wall of the upper cover 1 close to the limiting card platform 3, and an upper positioning strip 4.3 is arranged on the outer wall of the upper cover 1 located above the upper positioning strip 4.3. The spacing between the upper positioning strip 4.3 and the lower positioning strip 4.2 is equal to the spacing between the two annular positioning grooves 4.1, so as to ensure that the diaphragm 2 is punctured. 2, the upper positioning strip 4.3 and the lower positioning strip 4.2 are respectively mounted in the corresponding annular positioning groove 4.1, ensuring the positioning performance and sealing performance of the upper cover 1 and the upper connecting section 2.3; the lower positioning strip 4.2 is mounted in the upper annular positioning groove 4.1 when leaving the factory, which not only ensures that the cutting teeth 6 are located above the diaphragm 2.2 when not in use, avoiding the cutting teeth 6 from piercing the diaphragm 2.2 and causing the solid reagent and the liquid reagent to mix, but also ensures the sealing performance and positioning performance of the upper cover 1 and the upper connecting section 2.3 when leaving the factory;

[0023] like Figure 1 , 5 As shown, the film cutting structure includes a film cutting knife 5 extending vertically downward from the inner edge of the limiting clamping table 3 and having an annular structure. The bottom of the film cutting knife 5 includes a cutting part and a film pressing part. The cutting part is provided with a plurality of cutting teeth 6 extending downward along its circumference. The length of the film pressing part is L Equal to the circumference of the bottom of the film cutting knife C of 1 / 12 During the membrane cutting process, part of the membrane 2.2 is ensured to be still connected to the annular stopper 2.1 to prevent the membrane 2.2 from being completely separated from the annular stopper 2.1 and falling into the tube below to affect the amplification reaction. Figure 2 As shown;

[0024] like Figure 1 , 4 As shown, the tube body is sealed and inserted in the lower connecting section 2.4, and the tube body includes a wide-mouth section 7.1 and a thin tube section 7.2. The wide-mouth section 7.1 and the thin tube section 7.2 are connected as a whole through a truncated cone section 7.3. A clamping strip 8.1 is arranged on the upper part of the outer wall of the wide-mouth section 7.1, and an annular fixing groove 8.2 matched with the clamping strip 8.1 is provided in the lower connecting section 2.4 to ensure the sealing performance and positioning performance of the tube body and the lower connecting section 2.4. The present invention makes the tube body into a structure with a larger upper part and a smaller lower part, which not only ensures that the solid reagent falls smoothly to the truncated cone section 7.3 or the thin tube section 7.2, but also can concentrate the concentrated liquid reagent in the thin tube section 7.2 at the bottom for easy transportation, while avoiding the concentrated liquid from adhering to the wide-mouth section 7.1 and affecting the amplification reaction effect.

[0025] The specific use process of the present invention is described as follows:

[0026] First, the tube body is removed from the connecting sleeve by external power or manually, and then the sample and diluent are added to the tube body and the tube body is inserted into the connecting sleeve. Then, the upper cover 1 is pressed downward by external power or manually, and the cutting teeth 6 puncture the diaphragm 2.2 as the upper cover 1 descends. The state of the diaphragm 2.2 after being punctured is as follows: Figure 2 As shown, the freeze-dried beads and wax beads in the upper cover 1 smoothly enter the tube body and react with the mixed liquid in the tube body. The entire operation process only requires manual addition of samples and diluents, which simplifies the operation steps, shortens the operation time, and improves work efficiency. At the same time, it also greatly shortens the exposure time of the tube body, thereby reducing the chance of sample contamination and ensuring the reliability of the amplification reaction results.

Claims

1. A press-type reaction tube, comprising a tube body and an upper cover (1), characterized in that: The tube body and the upper cover (1) are sealed and connected as one body via a connecting sleeve; a diaphragm (2.2) is provided on the annular limit platform (2.1) in the connecting sleeve; the diaphragm (2.2) separates the connecting sleeve into an upper connecting section (2.3) for connecting the upper cover (1) and a lower connecting section (2.4) for connecting the tube body; the upper cover (1) is sealed and inserted in the upper connecting section (2.3), and the tube body is sealed and inserted in the lower connecting section (2.4); A limit clamping platform (3) matched with the annular limit platform (2.1) is arranged on the outer wall of the lower end of the upper cover (1), and a film cutting structure for puncturing the diaphragm (2.2) is arranged on the upper cover (1). The film cutting structure is located above the diaphragm (2.2), and comprises a film cutting knife (5) extending vertically downward from the inner edge of the limit clamping platform (3) and having an annular structure. The bottom of the film cutting knife (5) comprises a cutting portion and a film pressing portion. A plurality of cutting teeth (6) extending downward are arranged on the cutting portion along its circumference. The length L of the film pressing portion and the circumference C of the bottom circle of the film cutting knife satisfy the following relationship: 1 C / 72≤L≤1 C / 4; A limiting structure is provided between the upper cover (1) and the upper connecting section (2.3), the limiting structure comprising a pair of annular positioning grooves (4.1) arranged at intervals on the inner side wall of the upper connecting section (2.3), a lower positioning strip (4.2) matching the annular positioning groove (4.1) is provided on the outer side wall of the upper cover (1) close to the limiting clamping platform (3), an upper positioning strip (4.3) is provided on the outer side wall of the upper cover (1) located above the lower positioning strip (4.2), the spacing between the upper positioning strip (4.3) and the lower positioning strip (4.2) is equal to the spacing between the two annular positioning grooves (4.1), and the lower positioning strip (4.2) is clamped in the annular positioning groove (4.1) located above; The upper cover (1) contains a solid reagent, and the tube body contains a liquid reagent; The tube body comprises a wide-mouth section (7.1) and a thin tube section (7.2), wherein the wide-mouth section (7.1) and the thin tube section (7.2) are connected as a whole via a frustum section (7.3).

2. The push-type reaction tube according to claim 1, characterized in that: A clamping strip (8.1) is provided on the outer side wall of the wide-mouth section (7.1), and an annular fixing groove (8.2) matching with the clamping strip (8.1) is provided in the lower connecting section (2.4).

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