A trace fluorine phase liquid dripping device

Abstract

The present invention discloses a micro-fluorine phase liquid drop adding device, including a drop control system, an XYZ three-dimensional servo motor positioning system and a conveyor belt mobile positioning system, the drop control system includes a liquid reservoir, a three-channel converter, a syringe, a drop head and multiple catheters, the liquid reservoir, the syringe and the drop head are respectively connected to the three-channel converter through a catheter, the drop head adopts a needle tube with an anti-fingerprint treatment on the surface, and the inner diameter of the needle tube is 0.6-0.9 mm. The drop adding device is simple in structure and easy to operate, and a needle tube with surface modification is adopted to realize continuous and stable suspension and drop adding of liquid, and the drop adding device can be used to drop fluorine phase liquid in large quantities, and has good practicality.

Description

Technical Field

[0001] The invention relates to the technical field of liquid dripping, and in particular to a trace fluorine phase liquid dripping device. Background Art

[0002] The technology of micro-liquid drop addition is widely used in biochemical, medical and other experimental fields, because these experiments require a large amount of pipetting or sample addition operations every day, such as accurately transferring various liquids or samples in various volumes to various analytical or testing equipment for various preparations and tests. In recent years, with the advancement of technology, micro-liquid drop addition has also been applied to industrial production. For example, the coating particles for surface evaporation anti-fingerprint coating are a metal crucible filled with steel wool, and microgram-level anti-fingerprint agents are attached to the steel wool. These anti-fingerprint agents need to be added to the crucible by drop addition.

[0003] At present, the aspiration range of traditional micro-pipette is generally between 0.1-1000 microliters. Manual operation or automated continuous dilution is required for micro-pipette, which takes a long time and consumes a lot of samples. Internationally, acoustic wave technology is also used to vibrate the liquid from the liquid surface to the container above, which can achieve nanoliter-level sampling, but it requires the container to be placed upside down, which is not suitable for the situation where there is a lot of liquid in the container.

[0004] The invention patent with publication number CN209034304U discloses a micro-liquid dripping device that can drip liquid stably and evenly at a low cost. The dripping of micro-liquids is mainly achieved through precise control of mechanical parts such as gears, and multiple dripper devices can also drip several samples at the same time. However, this device cannot solve the problem of large-scale dripping.

[0005] For example, the Chinese invention patent with the authorization announcement number CN 202621132U discloses a liquid material dripping device, including a feed pipe and a valve installed on the feed pipe, the feed pipe is provided with a dripping device; the dripping device includes a clamping flange and a perforated gasket arranged in the middle of the clamping flange. The invention is simple to operate and can accurately control the amount of material dripping, but the device can only drip a single pre-drip component, and its working efficiency is low.

[0006] Chinese invention patent CN105344394A discloses a liquid diverter, and particularly relates to a high-precision micro-liquid equal-volume diverter. The diverter includes a cover plate and a substrate, wherein the cover plate is provided with a sample injection hole, and the substrate is provided with an arc-shaped liquid channel connected to the sample injection hole and a sample outlet hole connected to the liquid channel, and is characterized in that the liquid channel is surface-hydrophilic modified, and a circular liquid reservoir whose size decreases successively with the flow direction of the liquid is connected to the liquid channel, and each section of the liquid channel connecting the liquid reservoir, the sample injection hole and the sample outlet hole extends along the tangent direction of the circular liquid reservoir, and its access and access points to the circular liquid reservoir are located at the upper edge of the liquid reservoir and are symmetrically distributed. Chinese invention patent with publication number CN110488028A discloses an ultra-micro liquid loading device, including a loading module, and the loading module also includes a droplet exciter with a micro hole and a sample storage cavity, and the droplet exciter is electrically connected to the control module. The micropore unit is driven to vibrate by electrically connecting the control module through the droplet exciter. The sample in the sample storage cavity drips out of the micropore through vibration. One droplet can be discharged for each vibration. The minimum diameter of each micropore is 2 microns, which can achieve the loading of a single droplet of 0.03 picoliter. The number of vibrations is controllable. The droplet exciter can achieve high-frequency output of droplets. When using piezoelectric excitation technology, each micropore can output and load about 100,000 droplets per minute. The ultra-micro liquid loading device can achieve high-precision and high-efficiency ultra-micro liquid loading by setting different micropore sizes, the number of micropores and controlling the vibration frequency.

[0007] In particular, the liquids added in the fields of biochemistry, medicine, etc. are all water-based liquids, while anti-fingerprint liquids are generally prepared with fluorinated solvents, which have the characteristics of large specific gravity (1.3-1.8) and small surface tension (<20 dynes / cm). When such liquids are used in ordinary pipettes, leakage and other problems often occur due to the mismatch between the surface of the pipette tip and the liquid affinity, resulting in inaccurate sampling.

[0008] How to solve the above problems is what those skilled in the art are committed to solving. Summary of the invention

[0009] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a liquid dropping device for high specific gravity, low surface tension fluorine phase organic liquid, which can be automatically operated in batches and meet the needs of anti-fingerprint coating particle production.

[0010] To achieve the above-mentioned purpose, the technical solution adopted by the present invention is: a trace fluorine phase liquid dripping equipment, including a dripping control system, an XYZ three-dimensional servo motor positioning system and a conveyor belt moving positioning system, the dripping control system includes a liquid reservoir, a three-channel converter, a syringe, a dripping head and multiple catheters, the liquid reservoir, the syringe and the dripping head are respectively connected to the three-channel converter through catheters, the dripping head uses a needle tube with an anti-fingerprint treated surface, and the inner diameter of the needle tube is 0.6-0.9 mm.

[0011] Preferably, a piston is installed in the syringe, and the drip control system also includes a screw propeller connected to the end of the piston and used for regulating the movement stroke of the piston.

[0012] As a specific implementation, the accuracy of the screw propeller is 1±0.1 microns.

[0013] Preferably, the needle tube is a metal needle tube or a quartz glass needle tube.

[0014] As a specific implementation, the needle tube uses a metal needle tube with a modified surface, and the specific modification process is as follows: the surface of the metal needle tube is anodized and then washed and dried with deionized water; then it is immersed in anti-fingerprint liquid containing 0.1% Daikin UD509 and dried at 80°C for half an hour.

[0015] As a specific implementation, the needle tube adopts a quartz glass needle tube with a modified surface. The specific modification process is as follows: the surface of the quartz glass needle tube is treated with 1% sodium hydroxide dilute alkali solution for half an hour, then washed and dried with deionized water, and then immersed in anti-fingerprint liquid containing 0.1% Daikin UD509, and then dried at 80°C for half an hour.

[0016] Preferably, the conveyor belt moving positioning system comprises a conveyor frame, a conveyor belt mounted on the conveyor frame, a tray mounted on the conveyor belt and moving with the conveyor belt, and a liquid receiving crucible is placed on the tray.

[0017] Further preferably, the tray is made of acrylic material, and a groove for placing the liquid-receiving crucible is provided on the tray.

[0018] Preferably, the XYZ three-dimensional servo motor positioning system includes an X-axis moving frame, a Y-axis moving frame and a Z-axis moving frame which are perpendicular to each other, and the dripping head is installed on the Z-axis moving frame. There are multiple dripping heads, and the multiple dripping heads are arranged at equal intervals along the length direction of the Z-axis moving frame.

[0019] Due to the application of the above technical scheme, the present invention has the following advantages compared with the prior art: the trace fluorine phase liquid dripping equipment of the present invention adopts a dripping control system, an XYZ three-dimensional servo motor positioning system and a conveyor belt moving positioning system, and the dripping head used adopts a needle tube with an anti-fingerprint treated surface, and the inner diameter of the needle tube is 0.6-0.9 mm, so that the affinity of the needle head surface and the dripping liquid are matched, and the fluorine phase organic liquid with large contrast and small surface tension can be accurately dripped, and multiple dripping heads can be used to perform accurate and rapid equal amount diversion and transport control between multiple channels. During repetitive experiments, combined with an automated platform, the limitations of manual sample addition in terms of repeatability and accuracy can be overcome, which has important practical significance. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic structural diagram of the trace fluorine phase liquid dripping device of the present invention;

[0021] Among them: 1. Liquid reservoir; 2. Catheter; 3. Three-channel converter; 4. Syringe; 5. Screw propeller; 6. Dropping head; 7. XYZ three-dimensional servo motor positioning system; 8. Conveyor belt; 9. Tray; 10. Groove; 11. Conveying motor. DETAILED DESCRIPTION

[0022] The technical solution of the present invention is further described below in conjunction with the accompanying drawings and specific embodiments.

[0023] A trace fluorine phase liquid dripping device, including a dripping control system, an XYZ three-dimensional servo motor positioning system 7 and a conveyor belt moving positioning system, see Figure 1 shown.

[0024] The drip control system includes a liquid reservoir 1, a three-channel converter 3, a syringe 4, a drip head 6 and a plurality of catheters 2, see Figure 1 The liquid reservoir 1, the syringe 4 and the dropper 6 are connected to the three-channel converter 3 through the catheter 2, respectively. The dropper 6 uses a needle tube with an anti-fingerprint surface treatment, and the inner diameter of the needle tube is 0.6-0.9 mm.

[0025] Among them, the liquid reservoir 1 is used to store the fluorine phase liquid to be dripped; the three-channel converter 3 is mainly used to adjust the flow direction of the liquid and realize the operations of aspirating, dripping, and emptying together with the syringe 4; there are multiple catheters 2, which are respectively connected between the three-channel converter 3 and the liquid reservoir 1, the syringe 4, and the dripping head 6 to play a role in liquid transportation; the syringe 4, when the three-channel converter 3 is adjusted to connect the syringe 4 and the liquid reservoir 1, the piston in the syringe 4 moves downward to realize the liquid absorption from the liquid reservoir 1; the piston in the syringe 4 moves upward to return the liquid to the liquid reservoir 1; when the three-channel converter 3 is adjusted to connect the syringe 4 and the dripping head 6, the piston in the syringe 4 moves upward to realize the liquid dripping function or discharge the air, and the piston in the syringe 4 moves downward to realize the air inhalation function.

[0026] In this example, the drip control system further includes a screw propeller 5 connected to the piston end in the syringe 4 and used to adjust the piston movement stroke, see Figure 1 As shown. There can be multiple syringes 4 here, and the pistons in the multiple syringes 4 are connected to the same screw propeller 5 and controlled by the same screw propeller 5. The screw propeller 5 here is a high-precision screw propeller 5 with an accuracy of 1±0.1 microns, which is used to achieve precise control of the piston movement stroke. The drip control system also includes a controller installed on the screw propeller 5 and a computer connected to the controller for communication. The computer is installed with control software for transmitting control signals to the controller, and the stroke of the screw propeller 5 is accurately adjusted by the controller, thereby accurately adjusting the amount of liquid to be dripped.

[0027] Here, the needle tube can be a metal needle tube or a quartz glass needle tube.

[0028] When the needle tube is a metal needle tube, the needle tube surface modification process is as follows: the metal needle tube surface is anodized, then washed and dried with deionized water, and then immersed in anti-fingerprint liquid containing 0.1% Daikin UD509 and dried at 80°C for half an hour.

[0029] When the needle tube is made of quartz glass, the surface modification process of the needle tube is as follows: the surface of the quartz glass needle tube is treated with 1% sodium hydroxide dilute alkali solution for half an hour, washed and dried with deionized water, and then immersed in anti-fingerprint liquid containing 0.1% Daikin UD509, and dried at 80°C for half an hour.

[0030] In this example, a dropper head 6 is used, which adopts a metal needle tube or a quartz glass needle tube, and its surface is modified so that the needle tube surface has good affinity for the fluorine-containing solvent, which greatly improves the suspension of the fluorine-containing solvent on the needle surface, so that the fluorine-containing solvent liquid can flow continuously and stably.

[0031] In this example, the XYZ three-dimensional servo motor positioning system 7 includes an X-axis moving frame, a Y-axis moving frame and a Z-axis moving frame which are perpendicular to each other. Figure 1 As shown, the dripping head 6 is installed on the Z-axis moving frame. Here, there are multiple dripping heads 6, and the multiple dripping heads 6 are installed on the Z-axis moving frame at equal distances along the length direction of the Z-axis moving frame.

[0032] The conveyor belt 8 moving positioning system comprises a conveyor frame, a conveyor belt 8 mounted on the conveyor frame, and a tray 9 mounted on the conveyor belt 8 and moving with the conveyor belt 8, see Figure 1 As shown, a liquid receiving crucible (not shown in the figure) is placed on the tray 9. The tray 9 is made of acrylic material, and a groove 10 for placing the liquid receiving crucible is provided on the tray 9, and the groove 10 here matches the shape of the liquid receiving crucible.

[0033] Here, the XYZ three-dimensional servo motor positioning system 7 conveniently realizes the calibration of the needle and the liquid receiving crucible position by connecting the controller installed on the X-axis moving frame, the Y-axis moving frame and the Z-axis moving frame to the computer, and then using the mouse and the scroll wheel to control the dripping position of the dripping head 6. Specifically, the X-axis moving frame and the Y-axis moving frame are used to accurately locate the position of the liquid receiving crucible in the dripping tray 9, and the Z-axis moving frame is used to accurately adjust the height of the needle.

[0034] Here, the bottom end of the tray 9 can be fixed on the conveyor belt 8 by a fixing slot (not shown in the figure). Figure 1 As shown, there are four dripping heads 6, which are arranged at equal intervals on the Z-axis moving frame. There are multiple groups of liquid-receiving crucibles on the tray 9, and each group also has four liquid-receiving crucibles arranged at equal intervals. The spacing between adjacent dripping heads 6 is consistent with the spacing between adjacent liquid-receiving crucibles. The conveyor belt 8 moving positioning system also includes a conveying motor 11 installed on the conveying frame for driving the conveyor belt 8 to run. The input end of the conveying motor 11 is connected to a computer. The computer terminal can be programmed or manually controlled by a button to control the movement of the transmission motor 11, so that the conveyor belt 8 drives the tray 9 and the crucible to move a certain stroke or continuously.

[0035] During the specific operation, the tray 9 is inserted into the conveyor belt 8, and the receiving crucible is placed in the tray 9. The relative position of the dripping head 6 is adjusted by the XYZ three-dimensional servo motor positioning system 7, and the parameters such as the dripping amount and the travel of the conveyor belt 8 are set in the computer program. The four dripping heads 6 are controlled by key control or program control to drip liquid at the same time, and then the conveyor belt 8 is controlled to move so that the next group of receiving crucibles corresponds to the position of the dripping head 6. After they are in place, the next group of dripping is carried out. This cycle is repeated to achieve continuous and accurate dripping.

[0036] The entire trace fluorine phase liquid dripping equipment adopts a dripping control system, an XYZ three-dimensional servo motor positioning system 7 and a conveyor belt moving positioning system, which can accurately and quantitatively drip fluorine phase organic liquids with large relative weight and small surface tension, and can automatically control dripping in batches, with high automation and accuracy. A metal needle tube or a quartz glass needle tube with an inner diameter of 0.6-0.9 mm is used, and its surface is modified to make the needle surface have good affinity for fluorine-containing solvents, which greatly improves the suspension of fluorine-containing solvents at the needle head, so that the fluorine-containing solvent liquid can flow continuously and stably.

[0037] The above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with the technology to understand the content of the present invention and implement it accordingly, and they cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A trace fluorine phase liquid dripping device, characterized in that, The invention comprises a liquid drop control system, an XYZ three-dimensional servo motor positioning system and a conveyor belt moving positioning system. The liquid drop control system comprises a liquid reservoir, a three-channel converter, a syringe, a liquid drop head and a plurality of catheters. The liquid reservoir, the syringe and the liquid drop head are respectively connected to the three-channel converter through catheters. The liquid drop head adopts a needle tube with an anti-fingerprint treatment on the surface. The inner diameter of the needle tube is 0.6-0.9 mm. The needle tube adopts a metal needle tube or a quartz glass needle tube. When the needle tube adopts a metal needle tube with a modified surface, the specific modification process is as follows: the surface of the metal needle tube is anodized and then washed and dried with deionized water; then an anti-fingerprint treatment containing 0.1% Daikin UD509 is used. After being immersed in fingerprint liquid, it is dried at 80°C for half an hour; when the needle tube adopts a quartz glass needle tube with modified surface, the specific modification process is as follows: after the surface of the quartz glass needle tube is treated with 1% sodium hydroxide dilute alkali solution for half an hour, it is washed and dried with deionized water, and then immersed in anti-fingerprint liquid containing 0.1% Daikin UD509, and dried at 80°C for half an hour. The XYZ three-dimensional servo motor positioning system includes an X-axis moving frame, a Y-axis moving frame and a Z-axis moving frame which are perpendicular to each other. The dripping head is installed on the Z-axis moving frame. There are multiple dripping heads, and the multiple dripping heads are arranged at equal intervals along the length direction of the Z-axis moving frame.

2. The trace fluorine phase liquid dropping device according to claim 1, characterized in that: A piston is installed in the syringe, and the drip control system also includes a screw propeller connected to the end of the piston and used for regulating the movement stroke of the piston.

3. The trace fluorine phase liquid dropping device according to claim 2, characterized in that: The screw propeller has an accuracy of 1±0.1 microns.

4. The trace fluorine phase liquid dropping device according to claim 1, characterized in that: The conveyor belt moving positioning system comprises a conveyor frame, a conveyor belt installed on the conveyor frame, a tray installed on the conveyor belt and moving with the conveyor belt, and a liquid receiving crucible is placed on the tray.

5. The trace fluorine phase liquid dropping device according to claim 4, characterized in that: The tray is made of acrylic material and is provided with a groove for placing the liquid receiving crucible.

Images