An adaptive floating tank for biofilm growth and collection

Abstract

The present application discloses a biofilm growth and collection adaptive floating box, comprising: a floating frame surrounded by baffles on four sides without baffles on the top and bottom, and the density of the baffle material is less than that of water; a plurality of windmills with gradually decreasing heights are arranged inside; a telescopic rod capable of telescoping up and down, located above the floating frame; a biofilm culture device located inside the floating frame, slidably connected to the floating frame and capable of sliding up and down relative to the floating frame; a timing device including a humidity sensor, a control main board and a timer; it is arranged between the floating frame and the biofilm culture device, fixedly connected to the floating frame, located at the lower part of the floating frame, and the bottom surface is flush with the self-weight draft line of the floating frame. One technical effect of the present application is that the present application can realize the cultivation and collection of microbial biofilms under the conditions of river rise and fall, and record the number of river rises and falls and the effluent duration of the cultivated biofilms.

Description

Technical Field

[0001] The present application belongs to the field of bioengineering, and specifically, relates to an adaptive floating box for biofilm growth and collection. Background Art

[0002] Biofilm is a typical complex heterogeneous complex composed of living and non-living substances, organic and inorganic substances. The life cycle of biofilm includes the initial formation of a cell monolayer through physical-chemical interactions or secretion of extracellular matrix proteins, then proliferation in the monolayer, microbial attachment to form an active biofilm to maturity, and finally the separation and death of the biofilm caused by solute transfer caused by transformation or biodegradation of active organisms. In recent years, heavy metal pollution in aquatic ecosystems has become increasingly serious. Biofilm has become a major research hotspot due to its role in adsorbing and degrading pollutants. In addition, as a small biological community, biofilm can reflect the health of aquatic ecosystems to a certain extent, and can serve as a sensitive indicator of watershed environmental changes, watershed pollution and ecosystem health. Compared with physical and chemical methods, biological treatment and detection have the advantages of low cost, no secondary pollution, and the ability to comprehensively reflect the combined effects of environmental factors.

[0003] The impact of climate change and seasonal water level fluctuations form seasonal or intermittent rivers, change the river hydrology, and cause changes in water pH, COD, dissolved oxygen and permanganate index. The runoff volume and retention time in rivers or lakes change, directly affecting the precipitation of suspended matter and the retention of nutrients such as nitrogen and phosphorus. Temperature and pH directly affect the enzyme activity of microorganisms in biofilms. Changes in pH may also cause changes in cell membrane electronuclearity, thereby affecting the absorption of substances by biofilms. Therefore, it is necessary to combine the study of seasonal or intermittent rivers with the study of biofilms in natural waters. However, there is currently a lack of dynamic monitoring methods and related monitoring instruments for biofilms in seasonal or intermittent rivers. Therefore, it is necessary to provide an adaptive floating tank for biofilm growth and collection to explore the response process of biofilms to seasonal or intermittent rivers. Summary of the invention

[0004] An object of the present application is to provide a new technical solution for biofilm growth and collection in an adaptive buoyancy tank.

[0005] According to one aspect of the present application, the present application provides a biofilm growth and collection adaptive floating box, comprising:

[0006] The floating frame is surrounded by baffles on four sides, but has no baffles on the top and bottom, and the density of the baffle material is less than that of water; a plurality of windmills with decreasing heights are arranged inside, and the windmills are fixed to the baffles through brackets, and the rotating plane of the windmills is parallel to the end face of the floating frame;

[0007] A telescopic rod, which can be telescoped up and down, is located above the floating frame and is connected to a baffle on the side of the floating frame through a fixing claw;

[0008] The biofilm culture device is located inside the floating frame, is slidably connected to the floating frame, and can slide up and down relative to the floating frame; the bottom surface is a first metal mesh, the lower end is connected to the weight, and a glass slide is arranged inside; the upper end is provided with steel needles corresponding to the positions of the windmills;

[0009] The timing device includes a humidity sensor, a control mainboard and a timer; the timing device is arranged between the floating frame and the biofilm culture device, fixedly connected to the floating frame, located at the lower part of the floating frame, with the bottom surface flush with the deadweight waterline of the floating frame, a sponge is arranged at the bottom, a second metal mesh is arranged in the sponge, the humidity sensor is fixed on the second metal mesh, and the humidity sensor and the timer are electrically connected to the control mainboard respectively.

[0010] Optionally, guide holes are provided on the baffle plate on one side of the floating frame and the baffle plate opposite thereto; a guide column passing through the guide hole is provided on the outer side of the biofilm culture device, and a wing plate is provided at one end of the guide column passing through the guide hole to limit the movement of the biofilm culture device between the two baffle plates with guide holes; the guide column cooperates with the guide hole so that the biofilm culture device can slide up and down relative to the floating frame.

[0011] Optionally, the telescopic rod includes a connected cross bar and a vertical bar, the cross bar can be telescoped horizontally, the vertical bar can be telescoped up and down, and the vertical bar is connected to the floating frame.

[0012] Optionally, the cross bar and / or the vertical bar include an inner tube and a sleeve, the sleeve is sleeved on the inner tube, a flat head screw is connected to the sleeve, and when the flat head screw is tightened, relative displacement between the inner tube and the sleeve can be prevented.

[0013] Optionally, the fixing claw is a cross fixing claw.

[0014] Optionally, the glass slide is arranged vertically, and the biofilm culture device includes a plurality of glass slides arranged parallel to each other.

[0015] Optionally, the timing device also includes a memory, a sealed box and a horizontal plate located above the sponge, the memory is electrically connected to the timer and the control main board respectively, the timer, the control main board and the memory are arranged in the sealed box, and the sealed box is arranged on the horizontal plate.

[0016] Optionally, the sealed box includes a box cover and a box body, and also includes a control switch arranged on the outer side of the top of the box cover, and the control switch is electrically connected to the controlled mainboard.

[0017] Optionally, the control mainboard and the memory are arranged on the inner side of the top of the box cover; the box cover is buckled on the box body and is sealed and connected to the box body.

[0018] Optionally, the timing device further comprises a battery, wherein the battery is disposed in the sealed box, and the battery is electrically connected to the humidity sensor, the control mainboard and the timer respectively.

[0019] One technical effect of the present application is that the present application can realize the cultivation and collection of microbial biofilms under the conditions of river fluctuations, and record the number of river fluctuations and the length of time the cultured biofilms are discharged from the water. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0021] Figure 1 It is a schematic diagram of the main structure of some embodiments of the present application.

[0022] Figure 2 It is a schematic diagram of the side structure of some embodiments of the present application.

[0023] Figure 3 It is a schematic diagram of the top view structure of some embodiments of the present application.

[0024] Figure 4 It is a schematic diagram of the three-dimensional structure of some embodiments of the present application.

[0025] Figure 5 It is a schematic diagram of the structure of the timing device in some embodiments of the present application.

[0026] Figure 6 It is a schematic diagram of the cross-sectional structure of the sealing box in some embodiments of the present application.

[0027] In the figure: 1 floating frame, 11 baffle, 12 guide hole, 13 windmill, 14 bracket, 2 telescopic rod, 21 horizontal rod, 22 vertical rod, 23 fixing claw, 3 biofilm culture device, 31 weight, 32 guide column, 33 wing plate, 34 first metal mesh, 35 steel needle, 4 timing device, 41 sponge, 42 second metal mesh, 43 horizontal plate, 44 humidity sensor, 45 sealing box, 46 control main board, 47 timer, 48 memory, 49 control switch. DETAILED DESCRIPTION

[0028] The following will describe the implementation methods of the present application in detail with the help of accompanying drawings and examples, so that the implementation process of how the present application uses technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly.

[0029] According to one aspect of the present application, the present application provides a biofilm growth and collection adaptive floating box. In some embodiments, reference Figures 1 to 6 , including a floating frame 1, a telescopic rod 2, a biofilm culture device 3 and a timing device 4.

[0030] The floating frame 1 is surrounded by baffles 11 on all four sides, and has no baffles on the top and bottom. It is a rectangular parallelepiped structure with openings on the top and bottom. The floating frame 1 is made of buoyancy material, the density of which is less than that of water, such as existing materials such as foam plastics. A plurality of windmills 13 with decreasing heights are arranged inside the floating frame 1, and the rotating surface of the windmill 13 is parallel to the end face of the floating frame 1. The windmill 13 is fixed to the baffle 11 by a bracket 14. The bracket 14 can be a horizontal rod, and its two ends are fixedly connected to the baffle 11, such as a common form such as a bolt connection, and is located in the upper half of the floating frame 1, so as not to cause obstruction to the biofilm culture device 3.

[0031] The telescopic rod 2 can be telescoped up and down, is located above the floating frame 1, and is connected to the baffle 11 on the side of the floating frame 1 through a fixed claw 23, that is, one end of the telescopic rod 2 is connected to the fixed claw 23, and the telescopic rod 2 part of the structure can be telescoped up and down relative to the floating frame 1. In some embodiments, the fixed claw 23 can be a cross fixed claw 23, which is fixedly connected to the baffles 11 on all four sides. In some embodiments, the telescopic rod 2 includes a connected cross bar 21 and a vertical bar 22, and the cross bar 21 can be telescoped horizontally to facilitate adjustment of the distance of the floating frame 1 relative to the shore; the vertical bar 22 can be telescoped up and down, and the vertical bar 22 is connected to the floating frame 1, so that the floating frame 1 can move up and down with the cross bar 21 as the reference line. Further, the cross bar 21 or / and the vertical bar 22 include an inner tube and a sleeve, the sleeve is sleeved on the inner tube, and a flat head screw is connected to the sleeve, and when the flat head screw is tightened, it can prevent the relative displacement between the inner tube and the sleeve.

[0032] The biofilm culture device 3 is located inside the floating frame 1, and is slidably connected to the floating frame 1, and can slide up and down relative to the floating frame 1. In some embodiments, the baffle 11 on one side of the floating frame 1 and the baffle 11 opposite to it can be specifically provided with a guide hole 12; the outer side of the biofilm culture device 3 is provided with a guide column 32 passing through the guide hole 12, and one end of the guide column 32 passing through the guide hole is provided with a wing plate 33 to limit the movement of the biofilm culture device 3 between the two baffles 11 with the guide hole 12; the guide column 32 cooperates with the guide hole so that the biofilm culture device 3 can slide up and down relative to the floating frame 1; further, the guide hole 12 is a waisted hole, and the part of the guide column 32 passing through the guide hole 12 is the same width as the guide hole 12 or slightly smaller. Further, the guide column 32 can be fixed on the biofilm culture device 3 by threaded connection. Further, the bottom of the waisted hole is close to the bottom end of the baffle 11, and the strength can support the suspension of the biofilm culture device 3.

[0033] The bottom surface of the biofilm cultivation device 3 is a first metal mesh 34, which does not hinder the upward and downward flow of water. The lower end of the biofilm cultivation device 3 is connected to a weight 31 to ensure the stability of the biofilm cultivation device 3 in water. The weight 31 can be a lead block or the like. A glass slide (not shown) is arranged inside the biofilm cultivation device 3 to carry the biofilm. Further, the glass slide is arranged vertically, and the biofilm cultivation device 3 includes a plurality of glass slides arranged parallel to each other. Further, a plurality of pairs of grooves are symmetrically arranged inside the biofilm cultivation device 3, and each pair of grooves is used to clamp and fix one of the glass slides.

[0034] The upper end of the biofilm culture device 3 is provided with steel needles 35 corresponding to the positions of the windmills 13. The steel needles 35 can be of the same height; or the heights can be set in the opposite direction according to the windmills 13 with decreasing heights in order to reduce the absolute height between the windmills 13 with decreasing heights in turn.

[0035] The timing device 4 includes a humidity sensor 44, a control mainboard 46 and a timer 47. The timing device 4 is arranged between the floating frame 1 and the biofilm culture device 3, and is fixedly connected to the floating frame 1, for example, by screw connection, clamp fixation, etc. The timing device 4 is located at the lower part of the floating frame 1, and the bottom surface is flush with the deadweight waterline of the floating frame 1. A sponge 41 is arranged at the bottom of the timing device 4, and a second metal mesh 42 is arranged in the sponge 41, and the humidity sensor 44 is fixed on the second metal mesh 42. The humidity sensor 44 is provided with a preset threshold. The humidity sensor 44 and the timer 47 are electrically connected to the control mainboard 46 respectively. The timing device 4 can be a hollow box body in the upper half, and a frame with four sides and a bottom surface fully open in the lower half.

[0036] When the device is in use, a suitable position is selected near the shore to place the device, that is, after being lowered, the biofilm culture device 3 can be supported by the bottom of a water source such as a river bottom or a lake bottom, the water surface overflows the glass slide, the floating frame 1 is in a state of self-weight suspension, the sponge 41 at the bottom of the timing device 4 is in contact with the water surface, and the telescopic rod 2 has a margin for extending downward and shrinking upward. When the water recedes, the floating frame 1 moves down with the water surface, and the steel needle 35 moves upward relative to the floating frame 1. When the steel needle 35 contacts the lowest windmill 13 and pokes the bottom surface of the windmill (the bottom surface of the windmill is a whole sheet and is at the same horizontal plane), the windmill 13 stops rotating, and the upper end of the slide is exposed to the water surface; as the water surface continues to recede, when the steel needle 35 contacts the highest windmill 13 and pokes the bottom surface of the windmill, the lower end of the slide is exposed to the water surface, and the floating frame 1 stops moving downward relative to the biofilm culture device 3, the water in the sponge 26 gradually evaporates, the humidity sensor 44 exceeds the threshold, and the control mainboard 46 is started. The control mainboard 46 simultaneously starts the timer 47 to count and record the time. When the water rises, the floating frame 1 moves up, the broken piece 21 starts to immerse in the water again, the sponge 26 absorbs water and becomes wet, and the humidity range reaches the threshold, and the timing stops. After a period of culture time, the device is slowly lifted out of the water surface, the slide is removed, and the collection or observation of the biofilm is completed. After this process and completion, the number of drought stress and rehydration effects can be determined by the number of holes on the windmill 13 (the number of times the same position on the rotating windmill is poked is negligible), and the timer data can be exported to obtain the water-out time of the cultured biofilm. In the test where rainy weather is regarded as the immersion time, the top of the floating frame 1 is open; in the test where rainy weather is not regarded as the immersion time, the top of the floating frame 1 can be covered with a plastic film to prevent water from leaking, so as to prevent the humidity sensor 44 from being triggered when the water recedes.

[0037] In some embodiments, reference Figures 5 and 6 The timing device 4 further includes a memory 48, a sealing box 45 and a horizontal plate 43 located above the sponge 41. The memory 48 is electrically connected to the timer 47 and the control main board 46 respectively for storing data. The timer 47, the control main board 46 and the memory 48 are arranged in the sealing box 45 to prevent short circuit caused by water splashing. The sealing box 45 is arranged on the horizontal plate 43.

[0038] In some embodiments, reference Figures 5 and 6 The sealed box 45 includes a box cover and a box body, and also includes a control switch 49 arranged on the outside of the top of the box cover. The control switch 49 is electrically connected to the controlled main board 46 and is used to manually shut down the entire system through the control main board 46 outside the sealed box 45 when the experiment is finished.

[0039] In some embodiments, reference Figures 5 and 6The control mainboard 46, the timer 47 and / or the memory 48 are arranged on the inner side of the top of the box cover to prevent water from entering the sealed box 45 and damaging data and devices in case of accident. The box cover is buckled on the box body and sealed with the box body, for example, a waterproof gasket is used to achieve the sealing between the box cover and the box body, and the box cover and the box body are fixed by friction, which belongs to the conventional technical means in the field and will not be elaborated here.

[0040] In some embodiments, reference Figures 5 and 6 The timing device 4 further includes a battery, which is disposed in the sealed box 45. The battery is electrically connected to the humidity sensor 44, the control mainboard 46 and the timer 47 respectively, and the device provides power. In some cases, the timer 47 can be a timer with a waterproof design, and the battery can also be a battery with waterproof protection, and the electrical connection position can be waterproofed to prevent accidents.

[0041] In some embodiments, the timing device 4 may also include a distance sensor (not shown), which is arranged at the upper end of the floating frame 1 and is used to measure the distance between the floating frame 1 and the biofilm culture device 3. The distance sensor is electrically connected to the memory 48 and is kept normally open. The recorded data is read at a preset frequency such as 1 time / min. The data is saved by the memory 48. The distance data is saved as a supplement and compared with the observed number of windmill holes and the recorded time of the timer to perform data proofreading to improve the reliability of the recording of the device.

[0042] For example, certain words are used in the specification and claims to refer to specific components or methods. Those skilled in the art should understand that different regions may use different nouns to refer to the same component. This specification and claims do not use differences in names as a way to distinguish components. For example, "including" mentioned throughout the specification and claims is an open term, so it should be interpreted as "including but not limited to". "Approximately" means that within an acceptable error range, those skilled in the art can solve the technical problem within a certain error range and basically achieve the technical effect. The subsequent description of the specification is a preferred embodiment of the present application, but the description is for the purpose of illustrating the general principles of the present application, and is not used to limit the scope of the present application. The scope of protection of the present application shall be determined by the attached claims.

[0043] It should also be noted that the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a product or system including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such a product or system. In the absence of more restrictions, the elements defined by the sentence "comprises a ..." do not exclude the existence of other identical elements in the product or system including the elements.

[0044] The above description shows and describes several preferred embodiments of the invention, but as mentioned above, it should be understood that the invention is not limited to the form disclosed herein, and should not be regarded as excluding other embodiments, but can be used in various other combinations, modifications and environments, and can be modified within the scope of the invention concept described herein through the above teachings or the technology or knowledge of the relevant field. Changes and modifications made by those skilled in the art should be within the scope of protection of the claims attached to the invention without departing from the spirit and scope of the invention.

Claims

1. An adaptive floating box for biofilm growth and collection, characterized in that, it includes: A floating frame, surrounded by baffles on four sides, without baffles on the top and bottom, and the density of the baffle material is less than that of water; Multiple windmills with gradually decreasing heights are arranged inside, and the windmills are fixed on the baffles through brackets, and the rotation surface of the windmills is parallel to the end surface of the floating frame; A telescopic rod, which can be telescoped up and down, is located above the floating frame and is connected to the baffle on the side of the floating frame through a fixed claw; A biofilm culture device, located inside the floating frame, is slidably connected to the floating frame and can slide up and down relative to the floating frame; the bottom surface is a first metal mesh, the lower end is connected to a weight, and a glass slide is arranged inside; the upper end is provided with steel needles corresponding to the positions of the windmills one by one; A timing device, including a humidity sensor, a control main board and a timer; is arranged between the floating frame and the biofilm culture device, is fixedly connected to the floating frame, is located at the lower part of the floating frame, the bottom surface is flush with the self-weight draft line of the floating frame, a sponge is arranged at the bottom, and a second metal mesh is arranged in the sponge, the humidity sensor is fixed on the second metal mesh, and the humidity sensor and the timer are respectively electrically connected to the control main board; When this device is in use, select a position near the shore to place this device, that is, after lowering, the biofilm culture device can be supported by the bottom of the water source, the water surface floods over the glass slide, the floating frame is in a suspended state, the sponge at the bottom of the timing device contacts the water surface, and the telescopic rod has a margin for downward extension and upward contraction; when the water recedes, the floating frame moves down with the water surface, and the steel needle moves upward relative to the floating frame. When the steel needle touches the lowest windmill and punctures the bottom surface of the windmill, the windmill stops rotating. The bottom surface of the windmill is a whole sheet and is at the same horizontal plane, and the upper end of the glass slide is exposed above the water surface; as the water surface continues to recede, when the steel needle touches the highest windmill and punctures the bottom surface of the windmill, the lower end of the glass slide is exposed above the water surface. At this time, the floating frame stops moving downward relative to the biofilm culture device, the water in the sponge gradually evaporates, the humidity sensor exceeds the threshold, the control main board is started, and the control main board starts the timer to record the time at the same time; when the water rises, the floating frame moves up, the glass slide starts to be immersed in the water again, the sponge absorbs water and becomes wet, and when the humidity reaches the threshold, the timing stops; after a period of culture time, slowly lift this device out of the water surface, remove the glass slide, and complete the collection or observation of the biofilm; during this process and after the end, determine the number of times of drought stress and rehydration by the number of holes on the windmill. The same position punctured on the rotating windmill is ignored, and the timer data is exported to obtain the water outlet duration of the cultured biofilm; In the experiment where rainy days are regarded as immersion time, the top of the floating frame is open; in the experiment where rainy days are not regarded as immersion time, the top of the floating frame is covered with a plastic film to prevent water and prevent the humidity sensor from being triggered by the threshold in the state of water recession.

2. The adaptive floating box for biofilm growth and collection according to claim 1, characterized in that, On one side of the floating frame and the baffle opposite thereto, guiding holes are provided; on the outside of the biofilm cultivation device, guiding columns passing through the guiding holes are provided, and wing plates are arranged at one ends of the guiding columns passing through the guiding holes to limit the movement of the biofilm cultivation device between the two baffles with guiding holes; the cooperation between the guiding columns and the guiding holes enables the biofilm cultivation device to slide up and down relative to the floating frame.

3. The self-adaptive floating tank for biofilm growth and collection according to claim 1, wherein, the telescopic rod includes a connected cross bar and a vertical bar, the cross bar can be horizontally telescoped, the vertical bar can be vertically telescoped, and the vertical bar is connected to the floating frame.

4. The self-adaptive floating tank for biofilm growth and collection according to claim 3, wherein, the cross bar and / or the vertical bar includes an inner tube and a sleeve, the sleeve is sleeved on the inner tube, and a flat head screw is connected to the sleeve, and when the flat head screw is tightened, it can prevent relative displacement between the inner tube and the sleeve.

5. The self-adaptive floating tank for biofilm growth and collection according to claim 1, wherein, the fixing claw is a cross fixing claw.

6. The self-adaptive floating tank for biofilm growth and collection according to claim 1, wherein, the glass slide is arranged vertically, and the biofilm cultivation device includes a plurality of glass slides arranged in parallel with each other.

7. The self-adaptive floating tank for biofilm growth and collection according to claim 1, wherein, the timing device further includes a memory, a sealed box and a horizontal plate located above the sponge, the memory is electrically connected to the timer and the control main board respectively, the timer, the control main board and the memory are arranged in the sealed box, and the sealed box is arranged on the horizontal plate.

8. The self-adaptive floating tank for biofilm growth and collection according to claim 7, wherein, the sealed box includes a box cover and a box body, and further includes a control switch arranged on the outer side of the top of the box cover, and the control switch is electrically connected to the control main board.

9. The self-adaptive floating tank for biofilm growth and collection according to claim 8, wherein, the control main board and the memory are arranged on the inner side of the top of the box cover; the box cover is buckled on the box body and is hermetically connected to the box body.

10. The self-adaptive floating tank for biofilm growth and collection according to claim 9, wherein, the timing device further includes a storage battery, the storage battery is arranged in the sealed box, and the storage battery is electrically connected to the humidity sensor, the control main board and the timer respectively.

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