An incubation device for immunofluorescence detector

Abstract

The invention discloses an incubation device for an immunofluorescence detector, comprising a stacked card compartment, an intermediate incubation unit, and a continuous incubation unit, wherein the stacked card compartment is provided with an intermediate incubation unit at the rear, and the intermediate incubation unit is provided with a continuous incubation unit at the rear. The invention can be adapted to both fixed-time incubation and variable-time incubation, and effectively solves the problems of complex control, low reliability, difficulty in realizing high-speed automation, and poor adaptability in the prior art.

Description

Technical Field

[0001] The present invention relates to the field of in vitro diagnostic equipment, and in particular to an incubation device for an immunofluorescence detector. Background Art

[0002] Fully automatic immunoassay is based on the immunological reaction of antigen-antibody binding, uses enzyme labels, lanthanide labels or chemiluminescent agents to label antigens and antibodies, and through a series of cascade amplification reactions, links the light signal or electrical signal with the analyte concentration, etc., to analyze the antigen or antibody to be tested in human samples. It is mainly used in hospital laboratories, third-party independent laboratories, blood test centers and other institutions to perform quantitative, semi-quantitative or qualitative detection of the content of each analyte in human body fluids, and to diagnose infectious diseases, tumors, endocrine function, cardiovascular diseases, eugenics, and autoimmune diseases.

[0003] According to the test principle and mode, immunoassay can be generally divided into one-step method, delayed one-step method, two-step method, etc. The main test steps generally include adding samples and reagents, mixing reactants, incubation, washing and separation (Bound-Free, referred to as B / F), adding signal reagents, measurement, etc. The incubation steps in the above process are generally divided into two types of existing specific implementation technical solutions: fixed time incubation and variable time incubation. Under the fixed time incubation mode, all test incubation times for each test mode are the same. For example, all one-step tests can only achieve 20 minutes of incubation, and all two-step tests can only achieve 10 minutes of incubation for the first time and 10 minutes for the second time. Due to the differences in reagent raw materials, formulas, production processes, reaction principles and conditions for specific test items, this fixed time incubation will increase the difficulty of reagent development or sacrifice certain test performance, such as sensitivity, in actual development and testing, and it is difficult to adapt to multiple different test items. Compared with the constraints and restrictions of the fixed-time incubation method on reagent development and performance, the variable-time incubation method is flexible and highly applicable. The incubation time can be flexibly set according to the different test items, that is, each test item can achieve its own most suitable incubation time. This method can reduce the constraints on reagent development and give full play to the performance of the reagent. In order to achieve variable-time incubation, the existing technical solutions generally use an independent incubation tray that can only achieve incubation. The incubation tray needs to be rotated and stopped multiple times in a test cycle, and the angle of each rotation is determined according to the incubation time. This technical solution has the disadvantages of complex control, high technical implementation difficulty, and unsuitability for high-speed automated testing.

[0004] In addition, the patent number 201710010509.8 discloses a reaction incubation unit, an immunoanalyzer, and a reaction incubation method, wherein the reaction incubation unit includes: a reaction unit for carrying and incubating a reaction container; a transfer unit for moving the reaction container in and out of the reaction unit; the reaction unit includes a rotating unit, an incubation position is set on the rotating unit, and the incubation position is progressively advanced by a predetermined angle θ with the rotating unit at fixed intervals T; the transfer unit moves the reaction container out of the incubation position according to a variable incubation time t1. Although this invention can achieve flexible and variable incubation time, it has the disadvantages of complex transfer unit, high cost, high control difficulty, and can only be adapted to variable time incubation. Summary of the invention

[0005] The purpose of the present invention is to overcome the common shortcomings and problems of the prior art and to provide a new incubation device with a simple structure and suitable for both fixed incubation time and variable incubation time.

[0006] The present invention is achieved through the following technical solutions:

[0007] An incubation device for an immunofluorescence detector comprises a stacked card compartment, an intermediate incubation unit and a continuous incubation unit. The stacked card compartment is provided with an intermediate incubation unit at the rear, and the intermediate incubation unit is provided with a continuous incubation unit at the rear.

[0008] The stacked card warehouse includes a box body, an exhaust port, a grid hole, an exhaust fan, a cooling fan, a left guide rail, a right guide rail, a partition, an upper card slot, a step block, a lower card slot, a reagent card box, and a handle. The box body is a rectangular parallelepiped with an opening in the front. The box body is composed of a left side plate, a right side plate, a lower side plate, an upper side plate and a back plate. The left side plate is equipped with a left guide rail, the right side plate is equipped with a right guide rail, the upper surface of the upper side plate is equipped with an exhaust port, an exhaust fan is installed at the exhaust port, the back plate is equipped with a grid hole, a cooling fan is installed at the grid hole, a plurality of upper card slots are provided on the upper side plate of the box body, and the upper card slots are evenly spaced by a plurality of partitions The box body is composed of a cloth, a plurality of lower card slots are arranged on the lower side plate, the lower card slots are composed of a plurality of step blocks evenly spaced, the step blocks are composed of a first step and a second step, the upper card slots correspond to the lower card slots one by one and are adapted to each other, the interval between the upper card slots and the lower card slots on the same vertical line forms a card slot cavity, a reagent card box is movably installed in the card slot cavity, the reagent card box is a rectangular structure with a hollow interior, an open slot is arranged on the bottom surface of the reagent card box, a handle is installed on the upper part of the front of the reagent card box, and a square hole connected to the open slot for convenient hooking is arranged on the lower part of the front of the reagent card box.

[0009] The intermediate incubation unit includes a first frame, a first motor, a first screw rod, a first screw rod nut, a first bottom plate, a first slider, a first guide rail, a vertical plate, a transition card seat, a second frame, a second bottom plate, a second motor, a second screw rod, a second screw rod nut, a first connecting block, a second slider, a second guide rail, a through hole, and a connecting rod;

[0010] The first and second guide rails are connected to each other via a plurality of cams, and the plurality of cams have respective opposite ends. The plurality of cams have respective opposite ends. The plurality of cams have respective opposite ends. The plurality of cams have respective opposite ends. The bottom of the right card plate is fixedly connected, and a guide groove opening upward is provided on the second connecting block, a pull rod which can slide back and forth is provided in the guide groove, and a hook head is provided at the front end of the pull rod; a left card groove with an opening facing the gap direction is provided on the inner side of the left card plate, and a right card groove with an opening facing the gap direction is provided on the inner side of the right card plate, the left card groove and the right card groove have the same width and height, and the openings are opposite, and the left card groove and the right card groove are symmetrically distributed along the guide groove, the height of the left card groove and the right card groove is adapted to the thickness of the reagent card, and the spacing between the left side surface of the left card groove and the right side surface of the right card groove is adapted to the width of the reagent card, the front end of the left card groove and the front end of the right card groove are both provided with an oblique inlet for introducing the reagent card, and the rear end of the left card groove and the rear end of the right card groove are both provided with an inclined oblique outlet for withdrawing the reagent card;

[0011] A second base plate is provided at the rear of the first frame, the second base plate is fixedly connected to the first base plate, a second motor is installed on the second base plate, the output shaft of the second motor is connected to the second screw rod, a matching second screw rod nut is provided on the second screw rod, the second screw rod nut is connected to the first connecting block, a second slider is installed on the bottom surface of the first connecting block, the second slider is movably installed on the second guide rail and can slide back and forth along the second guide rail, the first connecting block is fixedly connected to the connecting rod, and the connecting rod is fixedly connected to the pull rod.

[0012] The continuous incubation unit includes a second frame, a third motor, a first driving wheel, a first belt, a first passive wheel, a clamp, and a second slot. The third motor is installed at the left end of the second frame, the output shaft of the third motor is connected to the first driving wheel, the first driving wheel is connected to the first passive wheel through the first belt, a plurality of evenly distributed clamps are installed on the first belt through countersunk screws, a second slot is provided in the clamp, and the size of the second slot is adapted to the reagent card.

[0013] Furthermore, the novel incubation device also includes a scanning unit, which includes a third base plate, a fourth motor, a second driving wheel, a second belt, a second passive wheel, a belt clamp, and a fluorescent signal collector. The third base plate is installed on the top of the second frame, and the fourth motor is installed on the third base plate. The output shaft of the fourth motor is connected to the second driving wheel, and the second driving wheel is connected to the second passive wheel through the second belt. A belt clamp is installed on the second belt, and the belt clamp is fixedly connected to the fluorescent signal collector.

[0014] Furthermore, the novel incubation device also includes a card pushing unit, which includes a fifth motor, a third screw rod, a third screw rod nut, a third connecting block, a third slider, a third guide rail, and a lever. The fifth motor is fixedly mounted on the third base plate, and the output shaft of the fifth motor is connected to the third screw rod. The third screw rod is provided with a third screw rod nut matching therewith, and the third screw rod nut is fixedly connected to the third connecting block. A third slider is installed at the bottom of the third connecting block, and the third slider is located on the third guide rail and can slide back and forth along the third guide rail. The third guide rail is fixedly mounted on the third base plate, and a lever for pulling out the reagent card from the card clamp is installed on the side of the third connecting block.

[0015] The novel incubation device of the present invention is different from the existing disc rotating and card inserting incubation units. The present invention is an intermediate card bin incubation unit, which mainly includes a stacked card bin, an intermediate incubation unit, and a continuous incubation unit. The stacked card bin is composed of multiple groups of card slots, each group of card slots is equipped with a reagent card box, and each reagent card box has multiple stacked reagent cards, which are continuously pulled out from the bottom by a pull rod under the action of gravity or elastic force. A large number of reagent cards are stored in the stacked card bin, and a lower card slot and a square hole through which the pull rod can pass are provided at the bottom. The stacked card bin has the advantages of large card loading capacity, rapid card output, automatic falling of reagent cards, and suitability for high-speed automated detection.

[0016] The continuous incubation unit of the present invention comprises a third motor, a first driving wheel, a first belt, a first passive wheel, and a clamp. The first belt rotates continuously under the drive of the third motor, and the clamps are evenly distributed on the first belt. Therefore, the incubation time interval is fixed, which is suitable for a fixed-time incubation mode; the intermediate incubation unit of the present invention comprises a first frame, a first motor, a first screw rod, a first screw rod nut, a first bottom plate, a first slider, a first guide rail, a vertical plate, a transition seat, a second frame, a second bottom plate, a second motor, a second screw rod, a second screw rod nut, a first connecting block, a second slider, a second guide rail, a through hole, and a connecting rod. When the second motor in the intermediate incubation unit is started, the second screw rod, the second screw rod nut The first connecting block is driven to slide back and forth along the second guide rail, and the first connecting block then drives the connecting rod, the pull rod, and the hook head to move back and forth, so as to take out the reagent card from the stacked card compartment and send it into the transition card holder. The incubation time in the filter card holder can be controlled by the residence time of the reagent card in the transition card holder. If no intermediate incubation is required, the reagent card can be directly sent into the card clamp of the continuous incubation unit; if intermediate incubation is required, assuming that the intermediate incubation time is △t1, the reagent card can be first incubated for the residence time t1 in the transition card holder, and then sent to the card clamp of the continuous incubation unit for continued incubation for a fixed time. Assuming that the fixed incubation time is t2, the total incubation time of the reagent card is △t1 + t2, where △t1 is a variable, which can be determined according to different samples and reagents. Therefore, the present invention is suitable for both fixed-time incubation and variable-time incubation, and can be generally used for one-step method, delayed one-step method, and two-step method. It has a wide range of applicability and good flexibility, can save customers a lot of equipment investment, and can also reduce the operating troubles of inspectors.

[0017] In summary, the present invention has the advantages of large card loading capacity, rapid card output, automatic reagent card falling, and suitability for high-speed automated detection. It is simple and reliable, has lower cost, and is more convenient to operate. It effectively solves the problem that the existing incubation unit cannot be simultaneously adapted to multiple incubation methods such as one-step method, delayed one-step method, and two-step method. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the structure of the present invention.

[0019] Figure 2 It is a structural schematic diagram of the stacked card bin.

[0020] Figure 3 A top view of the stacked card compartment.

[0021] Figure 4 Schematic diagram of the structure of the reagent cartridge.

[0022] Figure 5 Schematic diagram of the structure of the intermediate incubation unit.

[0023] Figure 6 It is a structural schematic diagram of the transition card seat.

[0024] Figure 7 It is a schematic diagram of the structure of the continuous incubation unit in the present invention.

[0025] Figure 8 It is a schematic diagram of the structure of the scanning unit in the present invention.

[0026] Fig. 9 It is a structural schematic diagram of the card dialing unit in the present invention.

[0027] In the accompanying drawings, 1. stacked card compartment, 101. box, 102. exhaust port, 103. grid hole, 104. exhaust fan, 105. cooling fan, 106. left guide rail, 107. right guide rail, 108. partition, 109. second step, 110. upper card slot, 111. step block, 112. lower card slot, 113. reagent card box, 114. handle, 115. left side plate, 116. right side plate, 117. upper side plate, 118. lower side plate, 119. back plate, 120. card slot cavity, 121. square hole, 122. strip groove, 123. strip plate, 124. first step; 2. intermediate incubation unit, 201. first rack, 202 .The first motor, 203. The first screw rod, 204. The first screw rod nut, 205. The first bottom plate, 206. The vertical plate, 207. The transition seat, 208. The first slider, 209. The first guide rail, 210. The second bottom plate, 211. The second motor, 212. The second screw rod, 213. The second screw rod nut, 214. The first connecting block, 215. The second slider, 216. The second guide rail, 217. The connecting rod, 218. The pull rod, 219. The left card plate, 220. The right card plate, 221. The left card slot, 222. The right card slot, 223. The second connecting block, 224. The guide slot, 225. The hook head, 226. The oblique entrance, 227. The oblique exit; 3. Continuous incubation unit, 301. Second frame, 302. Third motor, 303. First driving wheel, 304. First belt, 305. First passive wheel, 306. Card clamp, 307. Second card slot; 4. Scanning unit, 401. Third bottom plate, 402. Fourth motor, 403. Second driving wheel, 404. Second belt, 405. Second passive wheel, 406. Fluorescence signal collector, 407. Belt clamp; 5. Card picking unit, 501. Fifth motor, 502. Third screw rod, 503. Third screw rod nut, 504. Third connecting block, 505. Third slider, 506. Third guide rail, 507. Pickup rod. DETAILED DESCRIPTION

[0028] The present invention will be further described in detail below through specific implementation modes in conjunction with the accompanying drawings.

[0029] like Figure 1As shown, an incubation device for an immunofluorescence detector includes a stacked card compartment 1, an intermediate incubation unit 2, and a continuous incubation unit 3. The intermediate incubation unit 2 is provided at the rear of the stacked card compartment 1, and the continuous incubation unit 3 is provided at the rear of the intermediate incubation unit 2.

[0030] like Figure 2 , Figure 3 , Figure 4 As shown, the stacked card compartment 1 includes a box body 101, an exhaust port 102, a grid hole 103, an exhaust fan 104, a cooling fan 105, a left guide rail 106, a right guide rail 107, a partition 108, an upper card slot 110, a step block 111, a lower card slot 112, a reagent card box 113, and a handle 114.

[0031] The box body 101 is welded by a left side plate 115 , a right side plate 116 , a lower side plate 117 , an upper side plate 118 and a back plate 119 . The left side plate 115 is welded with a left guide rail 106 , and the right side plate 116 is welded with a right guide rail 107 .

[0032] The upper surface of the upper side plate 118 is provided with an exhaust port 102, and an exhaust fan 104 is fixedly installed at the exhaust port 102 by screws. The back plate 119 is provided with a grid hole 103, and a cold air fan 105 is installed at the grid hole 103 by bolts. By delivering cold air from the grid hole 103 to the box body 101 through the cold air fan 105, the entire box body 101 is ensured to be in a low temperature state, thereby extending the service life of the reagent card. The exhaust fan 104 delivers gas from the exhaust port 102, thereby achieving an air convection effect and ensuring the air cleanliness of the entire box body 101.

[0033] The inner side of the upper side plate 118 is provided with a plurality of upper slots 110, and the upper slots 110 are composed of a plurality of uniform-sized partitions 108 evenly spaced, and the partitions 108 are fixed to the inner wall above the box body 101 by welding or screws. A plurality of lower slots 112 are provided on the lower side plate 117 at the bottom of the box body 101, and the lower slots 112 are composed of a plurality of uniform-sized step blocks 111 evenly spaced, and the step blocks 111 are fixed to the lower side plate 117 at the lower end of the box body 101 by welding or bolts. The step blocks 111 will be composed of a first step 124 and a second step 109, and the upper slots 110 correspond to the lower slots 112 one by one and are adapted to each other, and the interval between the upper slots 110 and the lower slots 112 on the same vertical line constitutes a slot cavity 120. A reagent cartridge 113 is arranged in the slot cavity 120.

[0034] The reagent cartridge 113 is a rectangular parallelepiped with a hollow interior and no back plate, and a handle 114 is installed on the front of the reagent cartridge 113 by welding or injection molding. A square hole 121 is provided at the lower end of the front of the reagent cartridge 113, and the bottom surface of the reagent cartridge 113 is composed of two strip plates 123 and a strip groove 122, and the strip groove 122 is located between the two strip plates 123. Preferably, the width of the strip groove 122 is equal to the width of the square hole 121. The two strip plates 123 on the bottom surface of the reagent cartridge 113 are respectively located on the first steps 124 of two adjacent step blocks 111.

[0035] When the reagent card box 113 is filled with reagent cards and then inserted into the card slot cavity 120, since the strip plates 123 on the bottom surface of the reagent card box 113 are respectively located on the first steps 124 of two adjacent step blocks 111, the pull rod 218 can be used to hook out the reagent cards one by one from the square hole 121 in order. The stacked card compartment 1 stores a large number of reagent cards, and the bottom is provided with a lower card slot 112 and a square hole 121 through which the pull rod 218 can pass, which has the advantages of large card loading capacity, rapid card output, automatic drop of reagent cards, and is suitable for high-speed automated detection.

[0036] like Figure 5 , Figure 6 As shown, the intermediate incubation unit 2 includes a first frame 201, a first motor 202, a first screw rod 203, a first screw rod nut 204, a first base plate 205, a first slider 208, a first guide rail 209, a vertical plate 206, a transition seat 207, a second base plate 210, a second motor 211, a second screw rod 212, a second screw rod nut 213, a first connecting block 214, a second slider 215, a second guide rail 216, and a connecting rod 217.

[0037] A first motor 202 is installed at the right end of the first frame 201, and the output shaft of the first motor 202 is connected to a first screw rod 203, and a first screw rod nut 204 is provided on the first screw rod 203. The first screw rod nut 204 is welded to a first bottom plate 205, and two cylindrical first sliders 208 are installed on the bottom surface of the first bottom plate 205 by welding or bolt connection. The two first sliders 208 are movably installed on two mutually parallel round rod-shaped first guide rails 209, and can slide back and forth along the first guide rails 209, and both ends of the two first guide rails 209 are fixed on the first frame 201.

[0038] A vertical plate 206 is vertically welded and installed on one side of the surface of the first bottom plate 205, and a transition base 207 is installed on the upper part of the vertical plate 206 by welding or bolt connection. The transition base 207 includes a left card plate 219, a left card slot 221, a right card plate 220, a right card slot 222, a second connection block 223, a guide slot 224, a pull rod 218, a hook head 225, an oblique inlet 226, and an oblique outlet 227. The left card plate 219 and the right card plate 220 are arranged parallel and symmetrically. A long strip gap is provided between the left card plate 219 and the right card plate 220, and a long strip second connection block 223 is provided below the gap. The long strip second connection block 223 is welded and connected to the bottom of the left card plate 219 and the right card plate 220 respectively. The second connecting block 223 is provided with an upwardly opening guide groove 224, the guide groove 224 is a rectangular guide groove 224, a pull rod 218 that can slide back and forth is arranged in the guide groove 224, and a hook head 225 is installed at the front end of the pull rod 218. Preferably, the hook head 225 is trapezoidal, and the hook head 225 is a telescopic hook head 225 or a rotating hook head 225.

[0039] A left card slot 221 with an opening toward the gap direction is provided on the inner side of the left card plate 219, and a right card slot 222 with an opening toward the gap direction is provided on the inner side of the right card plate 220. The left card slot 221 and the right card slot 222 have the same width and height, and their openings are opposite. The left card slot 221 and the right card slot 222 are symmetrically distributed along the guide groove 224. The height of the left card slot 221 and the right card slot 222 is adapted to the thickness of the reagent card, and the distance between the left side surface of the left card slot 221 and the right side surface of the right card slot 222 is adapted to the width of the reagent card. The front end of the left card slot 221 and the front end of the right card slot 222 are both provided with an oblique inlet 226 for introducing the reagent card, and the rear end of the left card slot 221 and the rear end of the right card slot 222 are both provided with an inclined oblique outlet 227 for withdrawing the reagent card.

[0040] A second base plate 210 is provided at the rear of the first frame 201, and the second base plate 210 is fixedly connected to the first base plate 205 by welding. A second motor 211 is fixedly installed on the second base plate 210 by bolts, and the output shaft of the second motor 211 is connected to the second screw rod 212 by a coupling, and a second screw rod nut 213 is provided on the second screw rod 212. The second screw rod nut 213 is fixedly connected to the first connecting block 214 by welding or bolt connection, and a second slider 215 is installed on the bottom surface of the first connecting block 214 by welding or countersunk screw connection. The second slider 215 is movably installed on the second guide rail 216 and can slide back and forth along the second guide rail 216. The second guide rail 216 is parallel to the guide groove 224 in the transition seat 207, and the second guide rail 216 is fixedly installed on the second base plate 210 by countersunk screws. A connecting rod 217 is welded to the right side of the first connecting block 214 , and the connecting rod 217 is welded to the rear end of the pull rod 218 .

[0041] like Figure 7 As shown, the continuous incubation unit 3 includes a second frame 301, a third motor 302, a first driving wheel 303, a first belt 304, a first driven wheel 305, a clamp 306, and a second slot 307. The third motor 302 is installed at the left end of the second frame 301. The output shaft of the third motor 302 is connected to the first driving wheel 303. The first driving wheel 303 is connected to the first driven wheel 305 through the first belt 304. A number of evenly distributed clamps 306 are installed on the first belt 304 through countersunk screws. The clamp 306 is provided with a second slot 307 for placing a reagent card. The size of the second slot 307 is adapted to the reagent card.

[0042] When the second motor 211 is started, the first connecting block 214 is driven to slide back and forth along the second guide rail 216 through the second screw rod 212 and the second screw rod nut 213, and the first connecting block 214 then drives the connecting rod 217, the pull rod 218, and the hook head 225 to move back and forth, so that the reagent card is taken out from the stacked card compartment 1 and sent to the transition card seat 207. The incubation time in the filter card seat can be controlled by the residence time of the reagent card in the transition card seat 207. If no intermediate incubation is required, the reagent card can be directly sent to the clamp 306 of the continuous incubation unit 3; if intermediate incubation is required, assuming that the intermediate incubation time is △t1, the reagent card can be first incubated for the residence time t1 in the transition card seat 207, and then sent to the clamp 306 of the continuous incubation unit 3 for continued fixed time incubation. Assuming that the fixed incubation time is t2, the total incubation time of the reagent card is △t1 +t2, where △t1 is a variable, which can be determined according to different samples and reagents. Therefore, the present invention is suitable for both fixed-time incubation and variable-time incubation, and can be used for one-step method, delayed one-step method, and two-step method. It has a wide range of applications and good flexibility, which can save customers a lot of equipment investment and reduce the trouble of the tester's operation.

[0043] like Figure 8 As shown, the novel incubation device also includes a scanning unit 4, which includes a third bottom plate 401, a fourth motor 402, a second driving wheel 403, a second belt 404, a second passive wheel 405, a belt clip 407, and a fluorescent signal collector 406. The third bottom plate 401 is mounted on the top of the second frame 301 by welding or bolting, and the fourth motor 402 is mounted on the third bottom plate 401. The output shaft of the fourth motor 402 is connected to the second driving wheel 403, and the second driving wheel 403 is connected to the second passive wheel 405 by the second belt 404. The upper side of the second belt 404 is fixedly installed with a belt clip 407 by bolts and nuts, and the belt clip 407 is fixedly connected to the fluorescent signal collector 406 by countersunk screws or welding.

[0044] like Fig. 9As shown, the novel incubation device also includes a card-pulling unit 5, which includes a fifth motor 501, a third screw rod 502, a third screw rod nut 503, a third connecting block 504, a third slider 505, a third guide rail 506, and a lever 507. The fifth motor 501 is fixedly mounted on the third base plate 401 by bolts, the output shaft of the fifth motor 501 is connected to the third screw rod 502 by a coupling, the third screw rod 502 is provided with a third screw rod nut 503 matching therewith, the third screw rod nut 503 is fixedly connected to the third connecting block 504 by countersunk screws, the bottom of the third connecting block 504 is installed with a third slider 505 by welding or countersunk screw connection, the third slider 505 is located on the third guide rail 506 and can slide back and forth along the third guide rail 506, the third guide rail 506 is fixedly mounted on the third base plate 401 by countersunk screws, and the side of the third connecting block 504 is fixedly mounted with a lever 507 for removing the reagent card from the clamp 306 by countersunk screws. The lever 507 is a square rod, the upper end of which is welded to the third connecting block 504 , and the lower end of which extends into the clamp 306 of the continuous incubation unit 3 , and the lower end of which is slightly higher than the bottom surface of the clamp 306 .

[0045] The above-mentioned embodiments are only preferred implementations of the present invention, and their descriptions are relatively specific and detailed, but they cannot be understood as limiting the scope of the present invention. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. An incubation device for an immunofluorescence detector, characterized in that: It comprises a stacked card compartment, an intermediate incubation unit and a continuous incubation unit, wherein the intermediate incubation unit is arranged behind the stacked card compartment, and the continuous incubation unit is arranged behind the intermediate incubation unit; The stacked card warehouse includes a box body, an exhaust port, a grid hole, an exhaust fan, a cooling fan, a left guide rail, a right guide rail, a partition, an upper card slot, a step block, a lower card slot, a reagent card box, and a handle. The box body is a rectangular parallelepiped with an opening in the front. The box body is composed of a left side plate, a right side plate, a lower side plate, an upper side plate and a back plate. The left side plate is equipped with a left guide rail, the right side plate is equipped with a right guide rail, the upper surface of the upper side plate is provided with an exhaust port, an exhaust fan is installed at the exhaust port, the back plate is provided with a grid hole, a cooling fan is installed at the grid hole, and a plurality of upper card slots are provided on the upper side plate of the box body, and the upper card slots are evenly spaced by a plurality of partitions. The lower side plate of the box body is provided with a plurality of lower card slots, the lower card slots are formed by a plurality of step blocks evenly spaced, the step blocks are composed of a first step and a second step, the upper card slots correspond to the lower card slots one by one and are adapted to each other, the upper card slots and the lower card slots are on the same vertical line and are spaced to form a card slot cavity, a reagent card box is movably installed in the card slot cavity, the reagent card box is a rectangular parallelepiped structure with a hollow interior, an open slot is provided on the bottom surface of the reagent card box, a handle is installed on the upper part of the front of the reagent card box, and a square hole for convenient hooking and communicating with the open slot is provided on the lower part of the front of the reagent card box; The intermediate incubation unit includes a first frame, a first motor, a first screw rod, a first screw rod nut, a first base plate, a first slider, a first guide rail, a vertical plate, a transition card seat, a second frame, a second base plate, a second motor, a second screw rod, a second screw rod nut, a first connecting block, a second slider, a second guide rail, a through hole, and a connecting rod; a first motor is installed at the right end of the first frame, an output shaft of the first motor is connected to the first screw rod, a matching first screw rod nut is provided on the first screw rod, the first screw rod nut is connected to the first base plate, a first slider is installed on the bottom surface of the first base plate, and the first slider is movably installed on the first guide rail , and can slide back and forth along the first guide rail, and both ends of the first guide rail are fixed to the first frame; a vertical plate is vertically installed on one side of the surface of the first bottom plate, and a transition card seat is installed on the upper part of the vertical plate, and the transition card seat includes a left card plate, a right card plate, a second connecting block, a guide groove, a pull rod, a hook head, an oblique inlet, and an oblique outlet. The left card plate and the right card plate are arranged parallel and symmetrically, and a long strip gap is provided between the left card plate and the right card plate, and a second connecting block is provided below the gap, and the second connecting block is fixedly connected to the bottom of the left card plate and the right card plate respectively, and a guide groove opening upward is provided on the second connecting block, and the guide A pull rod that can slide back and forth is provided in the groove, and a hook head is provided at the front end of the pull rod; a left card slot with an opening facing the gap direction is provided on the inner side of the left card plate, and a right card slot with an opening facing the gap direction is provided on the inner side of the right card plate, the left card slot and the right card slot have the same width and height, and the openings are opposite, and the left card slot and the right card slot are symmetrically distributed along the guide groove, the height of the left card slot and the right card slot are adapted to the thickness of the reagent card, the distance between the left side surface of the left card slot and the right side surface of the right card slot is adapted to the width of the reagent card, the front ends of the left card slot and the right card slot are both provided with an oblique entrance for introducing the reagent card, and the The rear ends of the left card slot and the right card slot are both provided with inclined oblique exits for the reagent card to be withdrawn; a second bottom plate is provided at the rear of the first frame, the second bottom plate is fixedly connected to the first bottom plate, a second motor is installed on the second bottom plate, the output shaft of the second motor is connected to the second screw rod, a matching second screw rod nut is provided on the second screw rod, the second screw rod nut is connected to the first connecting block, a second slider is installed on the bottom surface of the first connecting block, the second slider is movably installed on the second guide rail and can slide back and forth along the second guide rail, the first connecting block is fixedly connected to the connecting rod, and the connecting rod is fixedly connected to the pull rod; The continuous incubation unit includes a second frame, a third motor, a first driving wheel, a first belt, a first passive wheel, a clamp, and a second slot. The third motor is installed at the left end of the second frame, the output shaft of the third motor is connected to the first driving wheel, the first driving wheel is connected to the first passive wheel through the first belt, a plurality of evenly distributed clamps are installed on the first belt, a second slot is provided in the clamp, and the size of the second slot is adapted to the reagent card.

2. An incubation device for an immunofluorescence detector according to claim 1, characterized in that: The incubation device also includes a scanning unit, which includes a third base plate, a fourth motor, a second driving wheel, a second belt, a second passive wheel, a belt clamp, and a fluorescent signal collector. The third base plate is installed on the top of the second frame, and the fourth motor is installed on the third base plate. The output shaft of the fourth motor is connected to the second driving wheel, and the second driving wheel is connected to the second passive wheel through the second belt. A belt clamp is installed on the second belt, and the belt clamp is fixedly connected to the fluorescent signal collector.

3. An incubation device for an immunofluorescence detector according to claim 1, characterized in that: The incubation device also includes a card pushing unit, which includes a fifth motor, a third screw rod, a third screw rod nut, a third connecting block, a third slider, a third guide rail, and a lever. The fifth motor is fixedly mounted on the third base plate, and the output shaft of the fifth motor is connected to the third screw rod. The third screw rod is provided with a third screw rod nut matching therewith, and the third screw rod nut is fixedly connected to the third connecting block. A third slider is installed at the bottom of the third connecting block, and the third slider is located on the third guide rail and can slide back and forth along the third guide rail. The third guide rail is fixedly mounted on the third base plate, and a lever for pulling out the reagent card from the card holder is installed on the side of the third connecting block.

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