A kit for large sections of pathological prostate tissue

Abstract

This invention discloses a large-section kit for pathological prostate tissue, comprising an embedding cassette, an embedding mold, and a sample holder disposed within a storage box. The embedding cassette includes an embedding cassette body, a first partition, a first elastic clamping device, an embedding cassette lid, and a mounting ring. The bottom plate of the embedding cassette body, the embedding cassette lid, and the first partition are all plate structures with mesh openings. The pathologically obtained prostate tissue is placed between the first partition and the bottom plate of the embedding cassette body, and the elastic clamping device is disposed between the first partition and the embedding cassette lid. The embedding mold includes an embedding mold body, a second partition, and a second elastic clamping device. This invention can effectively improve the integrity and consistency of slide preparation, reduce specimen loss, and ensure the homogeneity of continuous step slides. Based on this, various specific staining methods can be performed, improving the reliability of pathological clinical diagnostic assessment and the objectivity of biological information diversity analysis in basic research, thereby reflecting the reliability of the gold standard for pathological testing.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a kit for large sections of pathological prostate tissue. Background Technology

[0002] The Medical Prostate Tissue Paraffin Section Preparation and Storage Kit is a specialized set of instruments for material collection, dehydration, clearing, paraffin impregnation, embedding, sectioning, staining, and slide storage.

[0003] Only a few well-known hospitals in China have implemented large-section technology for radical prostatectomy specimens. The existing equipment is varied and there is no specially designed equipment. Some hospitals have purchased large tissue microtome (flat-push microtome) from companies such as Leica (Germany), Yamato (Japan), Sakura (Japan), or Thermo Fisher Scientific (USA). Others use existing paraffin rotary microtome with large chucks for paraffin sectioning. The embedding cassettes used are also relatively simple and have many defects, making them prone to shaking during sectioning. There is no specially designed tissue embedding cassette. No corresponding storage boxes for paraffin blocks and slides have been found. Summary of the Invention

[0004] The purpose of this invention is to provide a large slide kit for pathological prostate tissue to solve the problems existing in the prior art. It can effectively improve the integrity and consistency of slide preparation, reduce specimen loss, and ensure the homogeneity of continuous step slides. On this basis, various specific staining can be performed to improve the reliability of pathological clinical diagnosis and assessment and the objectivity of the analysis of biological information diversity in basic research, thereby reflecting the reliability of the gold standard for pathological testing.

[0005] To achieve the above objectives, the present invention provides the following solution:

[0006] This invention provides a large slide kit for pathological prostate tissue, including a storage box and an embedding box, an embedding mold, and a sample clip disposed within the storage box;

[0007] The embedding cassette includes an embedding cassette body, a partition, an elastic clamping device, an embedding cassette cover, and a mounting ring. The bottom plate of the embedding cassette body, the embedding cassette cover, and the partition are all plate structures with mesh. The partition is slidably connected to the embedding cassette body. The prostate tissue obtained after pathological sampling is placed between the partition and the bottom plate of the embedding cassette body. The elastic clamping device is placed between the partition and the embedding cassette cover. The mounting ring is placed at the bottom of the bottom plate of the embedding cassette body.

[0008] The embedding mold includes an embedding mold box body, a second partition, and a second elastic clamping device. The inner sidewall and bottom plate of the embedding mold box body are coated. The second partition is a plate structure with mesh. The second partition is slidably connected to the embedding mold box body. The prostate tissue, after dehydration, clearing, and wax impregnation, is placed between the second partition and the bottom plate of the embedding mold box body. A snap-fit ​​platform is provided at the top of the inner sidewall of the embedding box body. When the prostate tissue is embedded, the embedding box is snapped onto the top of the snap-fit ​​platform. The elastic clamping device is located between the mounting ring and the second partition.

[0009] The sample holder includes a dovetail groove and a clamping groove connected to the slicer, the clamping groove being used to hold an embedding cassette containing embedded prostate tissue.

[0010] Preferably, the embedding box body is a rectangular box structure, and each of the four inner side walls of the embedding box body is provided with a vertical groove. The partition is provided with a limiting slider around its perimeter, and the limiting slider is slidably connected to the corresponding groove.

[0011] Preferably, the embedding mold box body is a rectangular box structure, and each of the four inner side walls of the embedding box body is provided with a vertical sliding groove. The partition plate is provided with a limiting slider on all four sides, and the limiting slider is slidably connected to the corresponding sliding groove.

[0012] Preferably, the elastic clamping device is a spring with circular clamping caps at both ends, the top of the partition is provided with a ring, the bottom of the embedding box cover is provided with a ring, one clamping cap is installed in the ring at the top of the partition and the other clamping cap is installed in the ring at the bottom of the embedding box cover.

[0013] Preferably, the second elastic clamping device is a spring with circular clamping caps at both ends, and a ring is provided on the top of the second partition. One clamping cap is installed in the ring at the top of the first partition, and the other clamping cap is installed in the mounting ring at the bottom of the bottom plate of the embedding box body.

[0014] Preferably, the storage box is further provided with a glass slide, the glass slide including a glass slide body and a glass slide information identification area disposed on one side of the glass slide body, and a coverslip is placed on the prostate tissue on the glass slide.

[0015] Preferably, the carrier chip is further provided with horizontal and vertical scale lines; the carrier chip is also provided with position markers.

[0016] Preferably, the sample clip includes a sample clip body and a clamping rod. The dovetail groove is located at the tail end of the sample clip body, and the clamping groove is located at the head end of the sample clip body. The outline of the clamping groove matches the embedding cassette. The clamping rod is slidably connected to the sample clip body. The top of the clamping rod extends beyond the top of the sample clip body, and the bottom of the clamping rod is provided with a clamping part. The clamping part, in conjunction with the clamping groove, can clamp the embedding cassette containing embedded prostate tissue. A compression spring is provided between the clamping part and the sample clip body.

[0017] Preferably, the sample holder body is further provided with a universal fine-tuning device, which includes a fine-tuning sphere, an annular clamp, a locking spring, and a locking handle. The fine-tuning sphere has a spherical structure, and its spherical surface is embedded in the sample holder body. The end face of the fine-tuning sphere is connected to the dovetail groove. The annular clamp is fitted around the outside of the spherical surface of the fine-tuning sphere. One side of the annular clamp has an opening, and two locking rods are connected to the opening. The locking spring is connected between the two locking rods. The two locking rods are also provided with a semi-circular clamping groove and a rectangular clamping groove respectively. The locking handle includes a cylinder that mates with the semi-circular clamping groove and a rectangular block that mates with the rectangular clamping groove at its inner end. The outer end of the locking handle extends out of the sample holder body, and the body of the locking handle is rotatably connected to the sample holder body.

[0018] Preferably, the storage box includes a box body and a box cover disposed on the top of the box body, and the box body is provided with a compartment for placing the embedding box, the embedding mold, the sample clip, the glass slide and the coverslip.

[0019] The present invention achieves the following beneficial technical effects compared to the prior art:

[0020] 1. The pathological prostate tissue large section kit provided by this invention has sieve holes in the bottom plate, lid, and partitions of the embedding box to ensure the fluidity of reagents during dehydration and embedding, without affecting the quality of dehydration, clearing, paraffin infiltration, and embedding. Partitions are provided inside both the embedding box and the embedding mold, and the partitions are slidably connected to the sliding grooves on the box body, allowing the partitions to be stably pressed onto the prostate tissue large section. In particular, the elastic clamping device above the partition increases the stability of the partition and ensures that the prostate tissue large section remains as flat as possible and does not deform during dehydration in the embedding box. During embedding, the partitions ensure that the prostate tissue large section adheres flatly to the bottom of the embedding mold, reducing tissue loss during the paraffin sectioning trimming process and effectively improving its integrity.

[0021] 2. The pathological prostate tissue large section kit provided by the present invention has a coating around the embedding mold to facilitate rapid demolding after paraffin embedding of the tissue.

[0022] 3. The pathological prostate tissue large section kit provided by the present invention has a clamping groove and a holding part on the sample clip that cooperates with the embedding box, which can stably clamp the embedded prostate tissue. The middle adjustment body has a hemispherical universal micro-adjustment device, which can be locked by the locking handle through the ring locking ring. It can be trimmed when the paraffin block is sectioned to obtain the maximum tissue section surface and reduce tissue loss. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1 This is a schematic diagram of the structure of the large pathological prostate tissue slide kit in this invention;

[0025] Figure 2 This is a three-dimensional structural diagram of the embedding box in this invention;

[0026] Figure 3 This is a cross-sectional view of the embedding box in this invention;

[0027] Figure 4 This is a three-dimensional structural diagram of the embedding mold in this invention;

[0028] Figure 5 This is a three-dimensional assembly diagram of the embedding mold in the embedded state in this invention;

[0029] Figure 6 for Figure 5 Cross-sectional view;

[0030] Figure 7 This is a three-dimensional structural diagram of the sample clip in this invention;

[0031] Figure 8 for Figure 7 Cross-sectional view;

[0032] Figure 9 This is a three-dimensional structural diagram of the universal fine-tuning device in this invention;

[0033] Figure 10 This is a schematic diagram of the circular ring clamp in this invention;

[0034] Figure 11 This is a schematic diagram of the locking handle in this invention;

[0035] Figure 12 This is a schematic diagram of the structure of the glass slide and cover glass in this invention;

[0036] In the diagram: 1-Storage box, 11-Box body, 12-Box lid;

[0037] 2-Embedding box, 21-Embedding box body, 22-Partition 1, 23-Elastic clamping device 1, 24-Embedding box cover, 25-Mounting ring, 26-Slide groove 1, 27-Limiting slider 1;

[0038] 3-Embedding mold, 31-Embedding mold box body, 32-Second partition plate, 33-Second elastic clamping device, 34-Snap-fitting platform, 35-Second slide groove, 36-Second limiting slider;

[0039] 4-Sample clip, 41-Dovetail groove, 42-Clamping groove, 43-Sample clip body, 44-Clamping rod, 45-Clamping part, 46-Compression spring, 47-Universal fine-tuning device, 471-Fine-tuning ball, 472-Circular clamp, 473-Locking handle, 474-Locking rod, 475-Locking spring, 476-Semi-circular clamping groove, 477-Rectangular clamping groove, 478-Cylinder, 479-Rectangular block;

[0040] 5-Slide, 51-Information labeling area, 52-Scale line, 6-Cover slide, 7-Prostate tissue. Detailed Implementation

[0041] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0042] The purpose of this invention is to provide a large slide kit for pathological prostate tissue to solve the problems existing in the prior art.

[0043] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0044] The large pathological prostate tissue slide kit in this embodiment, such as... Figure 1 As shown, it includes a storage box 1 and an embedding box 2, an embedding mold 3, and a sample holder 4 disposed within the storage box 1;

[0045] like Figure 2 , Figure 3As shown, the embedding cassette 2 includes an embedding cassette body 21, a partition 22, an elastic clamping device 23, an embedding cassette cover 24, and a mounting ring 25. The bottom plate of the embedding cassette body 21, the embedding cassette cover 24, and the partition 22 are all plate structures with mesh. The mesh ensures the flowability of reagents during dehydration and embedding, without affecting the quality of dehydration and embedding. The partition 22 is slidably connected inside the embedding cassette body 21. The prostate tissue 7 obtained after pathological sampling is placed between the partition 22 and the bottom plate of the embedding cassette body 21. The elastic clamping device 23 is placed between the partition 22 and the embedding cassette cover 24. The mounting ring 25 is placed at the bottom of the bottom plate of the embedding cassette body 21. The embedding box body 21 is a rectangular box structure. Each of the four inner side walls of the embedding box body 21 is vertically provided with a sliding groove 26. The partition 22 is provided with limit sliders 27 around its perimeter. The limit sliders 27 are slidably connected to the corresponding sliding grooves 26. The limit sliders 27 are limited within the sliding grooves 26 to ensure the stability of the partition 22 and prevent it from shaking during embedding. The elastic clamping device 23 is a spring with circular clamping caps at both ends. The top of the partition 22 is provided with a ring, and the bottom of the embedding box cover 24 is provided with a ring. One clamping cap is installed in the ring at the top of the partition 22, and the other clamping cap is installed in the ring at the bottom of the embedding box cover 24.

[0046] like Figure 4-6 As shown, the embedding mold 3 includes an embedding mold box 31, a second partition 32, and a second elastic clamping device 33. The inner sidewalls and bottom plate of the box 11 of the embedding mold 3 are coated. The second partition 32 is a plate structure with mesh. The second partition 32 is slidably connected inside the embedding mold box 31. The dehydrated, transparent, and wax-impregnated prostate tissue 7 is placed between the second partition 32 and the bottom plate of the embedding mold box 31. The top of the inner sidewall of the embedding box 21 is provided with a locking platform 34. When the prostate tissue 7 is embedded, the embedding box 2 is locked onto the top of the locking platform 34. The elastic clamping device is located between the mounting ring 25 and the partition. Between 32; specifically, the embedding mold box 31 is a rectangular box structure, and each of the four inner side walls of the embedding box 21 is vertically provided with a sliding groove 35. The partition 32 is provided with a limiting slider 36 around its perimeter, and the limiting slider 36 is slidably connected to the corresponding sliding groove 35. The elastic pressing device 33 has the same structure as the elastic pressing device 23, and is a spring with round pressing caps at both ends. The top of the partition 32 is provided with a ring, one of which is installed in the ring at the top of the partition 22, and the other is installed in the mounting ring 25 at the bottom of the bottom plate of the embedding box 21.

[0047] like Figure 7As shown, the sample clip 4 includes a dovetail groove 41 and a clamping groove 42 connected to the microtome. The clamping groove 42 is used to clamp the embedding cassette 2 containing the embedded prostate tissue 7. The sample clip 4 includes a sample clip body 43 and a clamping rod 44. The dovetail groove 41 is located at the tail end of the sample clip body 43, and the clamping groove 42 is located at the head end of the sample clip body 43. The outline of the clamping groove 42 matches the embedding cassette 2. The clamping rod 44 is slidably connected to the sample clip body 43. The top of the clamping rod 44 extends out of the top of the sample clip body 43, and the bottom of the clamping rod 44 is provided with a clamping part 45. The clamping part 45, in conjunction with the clamping groove 42, can clamp the embedding cassette 2 containing the embedded prostate tissue 7. A compression spring 46 is provided between the clamping part 45 and the sample clip body 43.

[0048] The sample holder body 43 is also equipped with a universal fine-tuning device 47, such as Figure 8-11 As shown, the universal fine-tuning device 47 includes a fine-tuning ball 471, an annular clamp 472, a locking spring 475, and a locking handle 473. The fine-tuning ball 471 has a spherical structure, and its spherical surface is embedded in the sample holder body 43. The end face of the fine-tuning ball 471 is connected to the dovetail groove 41. The annular clamp 472 is fitted around the spherical surface of the fine-tuning ball 471. One side of the annular clamp 472 has an opening, and two locking rods 474 are connected to the opening. The locking spring 475 is connected between the two locking rods 474. The two locking rods 474 are also provided with a semi-circular clamping groove 476 and a rectangular clamping groove 477 respectively. The locking handle 473 includes an inner end with a cylinder 478 that mates with a semi-circular clamping groove 476 and a rectangular block 479 that mates with a rectangular clamping groove 477. The outer end of the locking handle 473 extends out of the sample clip body 43, and the body of the locking handle 473 is rotatably connected to the sample clip body 43. When using the universal fine-tuning device 47, rotating the locking handle 473 causes the rectangular block 479 to rotate. The rectangular block 479 rotates and opens the annular clamp 472, creating a gap between the annular clamp 472 and the fine-tuning ball 471. This allows adjustment of the angle of the entire sample clip body 43 relative to the dovetail groove 41, thereby fine-tuning the slice angle.

[0049] In this specific embodiment, a glass slide 5 is also provided inside the storage box 1, such as... Figure 12 As shown, the slide 5 includes a slide body and a slide information marking area 51 located on one side of the slide body. A coverslip 6 covers the prostate tissue 7 on the slide 5. The coverslip 6 is also provided with horizontal and vertical scale lines 52, which are used to mark the size of the tissue and facilitate the location of the lesion under the microscope. The slide 5 is also provided with position markers, including arrow markers and dot markers, which are used to mark the size of the tissue and facilitate the location of the lesion under the microscope.

[0050] In this specific embodiment, the storage box 1 includes a box body 11 and a box cover 12 disposed on the top of the box body 11. The box body 11 has a compartment for placing the embedding box 2, the embedding mold 3, the sample clip 4, the glass slide 5 and the cover glass 6.

[0051] The process of preparing large paraffin sections for prostate cancer using the pathological prostate tissue section kit provided by this invention is as follows:

[0052] (1) After tissue sampling, the tissue is placed in embedding box 2 (fixed in a fully automatic dehydrator, dehydrated with 75%-85%-95%-100% graded ethanol, cleared with xylene, and impregnated with paraffin at 56-58℃).

[0053] (2) Take out the embedding box 2 for embedding (the tissue is placed into the embedding mold 3 and 58-60℃ paraffin is poured); during the embedding process, the embedding box 2 is placed on the top of the embedding mold box 31. After the paraffin cools, it is taken out to form a wax block (a wax block with an embedding box).

[0054] (3) Slice the tissue. Place the embedding cassette 2 with the tissue wax block onto the sample clip 4. The sample clip 4 is then fixed onto the microtome. Slice the tissue at 4-6 μm. Place the sliced ​​tissue onto a glass slide 5 and stain it with hematoxylin and eosin. Then, cover the slide with a coverslip 6 with neutral resin.

[0055] This invention has illustrated its principles and implementation methods using specific examples. The descriptions of these embodiments are merely illustrative of the method and its core ideas; furthermore, those skilled in the art will recognize that modifications may be made to the specific implementation methods and application scope based on the principles of this invention. Therefore, the content of this specification should not be construed as limiting the invention.

Claims

1. A kit for large pathological prostate tissue sections, characterized in that: Includes a storage box and an embedding box, an embedding mold, and a sample holder disposed within the storage box; The embedding cassette includes an embedding cassette body, a partition, an elastic clamping device, an embedding cassette lid, and a mounting ring. The bottom plate of the embedding cassette body, the embedding cassette lid, and the partition are all plate structures with mesh. The partition is slidably connected to the embedding cassette body. The pathologically obtained prostate tissue is placed between the partition and the bottom plate of the embedding cassette body. The elastic clamping device is placed between the partition and the embedding cassette lid. The mounting ring is placed at the bottom of the bottom plate of the embedding cassette body. Each of the four inner sidewalls of the embedding cassette body has a vertically arranged groove. The partition is provided with limiting sliders around its perimeter, and the limiting sliders are slidably connected to the corresponding grooves. The embedding mold includes an embedding mold box body, a second partition plate, and a second elastic clamping device. The inner sidewalls and bottom plate of the embedding mold box body are coated. The second partition plate is a plate structure with mesh holes. The second partition plate is slidably connected to the embedding mold box body. The dehydrated, transparent, and wax-impregnated prostate tissue is placed between the second partition plate and the bottom plate of the embedding mold box body. A snap-fit ​​platform is provided at the top of the inner sidewall of the embedding mold box body. When the prostate tissue is embedded, the embedding box is snapped onto the top of the snap-fit ​​platform. The elastic clamping device is located between the mounting ring and the second partition plate. Each of the four inner sidewalls of the embedding mold box body has a vertically arranged sliding groove. The second partition plate has a limiting slider arranged opposite to each other around its perimeter. The limiting slider is slidably connected to the corresponding sliding groove. The sample holder includes a dovetail groove and a clamping groove connected to the slicer, the clamping groove being used to clamp an embedding cassette containing embedded prostate tissue; The sample clip includes a sample clip body and a clamping rod. The dovetail groove is located at the tail end of the sample clip body, and the clamping groove is located at the head end of the sample clip body. The outline of the clamping groove matches the embedding cassette. The clamping rod is slidably connected to the sample clip body. The top of the clamping rod extends beyond the top of the sample clip body, and the bottom of the clamping rod is provided with a clamping part. The clamping part, in conjunction with the clamping groove, can clamp the embedding cassette containing embedded prostate tissue. A compression spring is provided between the clamping part and the sample clip body. The sample holder body is also equipped with a universal fine-tuning device, which includes a fine-tuning sphere, an annular clamp, a locking spring, and a locking handle. The fine-tuning sphere has a spherical structure, and its spherical surface is embedded in the sample holder body. The end face of the fine-tuning sphere is connected to the dovetail groove. The annular clamp is fitted around the outside of the spherical surface of the fine-tuning sphere. One side of the annular clamp has an opening, and two locking rods are connected to the opening. The locking spring is connected between the two locking rods. The two locking rods are also provided with a semi-circular clamping groove and a rectangular clamping groove, respectively. The locking handle includes a cylinder that mates with the semi-circular clamping groove and a rectangular block that mates with the rectangular clamping groove at its inner end. The outer end of the locking handle extends out of the sample holder body, and the body of the locking handle is rotatably connected to the sample holder body.

2. The pathological prostate tissue large section kit according to claim 1, characterized in that: The embedding box has a rectangular box structure.

3. The pathological prostate tissue large section kit according to claim 1, characterized in that: The embedding mold body has a rectangular box structure.

4. The pathological prostate tissue large section kit according to claim 1, characterized in that: The elastic clamping device is a spring with circular clamping caps at both ends. A ring is provided at the top of the partition and a ring is provided at the bottom of the embedding box cover. One clamping cap is installed in the ring at the top of the partition and the other clamping cap is installed in the ring at the bottom of the embedding box cover.

5. The pathological prostate tissue large section kit according to claim 1, characterized in that: The second elastic clamping device is a spring with circular clamping caps at both ends. The top of the second partition is provided with a ring. One clamping cap is installed in the ring at the top of the first partition, and the other clamping cap is installed in the mounting ring at the bottom of the bottom plate of the embedding box body.

6. The pathological prostate tissue large section kit according to claim 1, characterized in that: The storage box also contains a glass slide, which includes a slide body and a slide information marking area located on one side of the slide body. A coverslip is placed over the prostate tissue on the slide.

7. The pathological prostate tissue large section kit according to claim 6, characterized in that: The slide is also provided with horizontal and vertical scale lines; the slide is also provided with position markers.

8. The pathological prostate tissue large section kit according to claim 7, characterized in that: The storage box includes a box body and a box cover disposed on the top of the box body. The box body has a compartment for placing the embedding box, the embedding mold, the sample clip, the glass slide and the coverslip.

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