Fully automatic fluorescent immunoassay analyzer and sample detection method

Abstract

The present invention discloses a fully automatic fluorescent immunoassay analyzer and a sample detection method. The analyzer includes a bracket, which is arranged on a base, and a sample introduction and sample discharging device is arranged on the base. The sample introduction and sample discharging device is used to push a sample tube into the sample tube for detection and push the sample tube out for sample discharging; a sample tube mixing component is arranged on the side of the sample introduction and sample discharging device, and the sample tube mixing component is used to mix the sample in the sample tube, and the sample tube is pushed into the sample tube for detection and pushed out for sample discharging by the sample introduction and sample discharging device; it also includes a sampling needle movement component for extracting the sample in the sample tube; a reagent compartment mechanism is arranged in the bracket, and a plurality of reagent cards are stacked in the reagent compartment mechanism, and the sampling needle adds the diluted sample to the sample adding port of the reagent card; an optical path detection component for detecting the reagent card is also arranged in the bracket. The overall layout of the analyzer is reasonable, compact, and fully functional, and it realizes fully automatic operation, humanized design, and is efficient and fast.

Description

Technical Field

[0001] The invention belongs to the field of fluorescent immunoassay detection, and in particular relates to a full-automatic fluorescent immunoassay analyzer and a sample detection method. Background Art

[0002] Fluorescence immunoassay is a commonly used detection technology in biomedical testing. This technology uses the characteristic that the analyzed substance will produce fluorescence under the stimulation of light of a specific wavelength to perform qualitative and quantitative detection. Due to its high sensitivity, strong specificity, fast detection speed, safety and stability, it is widely used in clinical testing and has broad application prospects in the detection of endocrine diseases, infectious diseases, obstetrics and gynecology, tumor markers, genetic diseases, blood and cytology.

[0003] Traditional fluorescent immunoassay analyzers are usually large in size and not convenient for handheld use. Therefore, they are limited in the fields of emergency testing (such as emergency laboratories, ICU, pediatrics, 120 ambulances, outdoor emergency rescue, individual rescue), inconvenient transportation, and economically underdeveloped areas. Even if there are some commercialized products, there are still disadvantages such as single detection band, complex structure, poor reliability, and low accuracy.

[0004] In Chinese patent document CN106198480A, an automatic dry fluorescence detector is disclosed, which includes: a sample in-and-out mechanism, including a sample transmission device, a test tube rack for placing multiple sample test tubes, and a test tube rack placement plate, the test tube rack is installed on the test tube rack placement plate, and the test tube rack can transport the sample test tube from the waiting position to the sampling position under the drive of the sample transmission device; a sampling mechanism, including a sampling device, a pipetting device, and a buffer zone for storing multiple buffer boxes, the sampling device is driven by the pipetting device to switch back and forth between the sampling position, the buffer box, and the loading position; a turntable mechanism, including a turntable, a turntable driving device for driving the loading plate to rotate, the turntable is provided with a plurality of card loading tongues for accommodating reagent cards along the radial direction, and one end of the card loading tongue extends to the edge of the turntable. The turntable is driven by a turntable driving device to rotate so that the card-carrying tongue reaches different angles and is located at a sample loading position, a detection position, a card dropping position and a card entry position, and there are at least two card entry positions; a card feeding mechanism comprises a card feeding device, a swinging device and at least two card boxes, each of which is circumferentially arranged on the outer periphery of the turntable, and the outlet of each card box can be opposite to a card entry position so that a corresponding position of each card box forms a card feeding position, the card feeding device is arranged on the outer periphery of the turntable and can rotate coaxially with the turntable, the swinging device is connected to the card feeding device and drives the card feeding device to rotate to reach different card feeding positions, and the card feeding device also comprises a card dialing component that can dial the reagent card in the card box into the card entry position along the radial direction of the turntable; the detection mechanism comprises a detection head for collecting data on the reagent card at the detection position.

[0005] The automatic dry fluorescence detector of the above technical solution is not very ideal in actual use, has poor humanized design and lacks functionality. Therefore, it is necessary to improve the existing fluorescence immunoassay device and develop a new fully automatic fluorescence immunoassay analyzer and sample detection method. Summary of the invention

[0006] The problem to be solved by the present invention is to provide a full-automatic fluorescent immunoassay analyzer.

[0007] In order to solve the above technical problems, the technical solution adopted by the present invention is that the fully automatic fluorescent immunoassay analyzer includes a bracket, the bracket is arranged on a base, and a sample injection and sample output device is arranged on the base, and the sample injection and sample output device is used to push the sample tube into the sample for testing and push the sample tube out for sample output;

[0008] A sample tube mixing component is arranged on the side of the sample injection and sample output device, and the sample tube mixing component is used to mix the sample in the sample tube, wherein the sample tube is pushed into the sample injection device for testing and pushed out for sample output;

[0009] It also includes a sampling needle motion assembly, which is used to extract the sample in the sample tube, and the sampling needle is installed on the sampling needle motion assembly;

[0010] A reagent compartment mechanism is arranged in the bracket, and a plurality of reagent cards are stacked in the reagent compartment mechanism. The sampling needle adds the diluted sample to the sample adding port of the reagent card;

[0011] The bracket is also provided with an optical path detection component for detecting the reagent card.

[0012] The fully automatic fluorescent immunoassay analyzer of the present invention arranges various components in a centralized manner, so that the overall layout of the analyzer is reasonable, compact, and fully functional, and fully automatic operation is achieved; the sample tube mixing component realizes the mixing function, and multiple reagent cards are stacked in the reagent compartment mechanism. After loading once, multiple uninterrupted tests can be performed; the optical path detection component is located in the bracket and is used to detect the fluorescent reaction signal of the reagent card. It has comprehensive functions, humanized design, and is efficient and fast.

[0013] Preferably, the sample injection and sample discharge device includes a sample injection push handle structure, a sample injection horizontal push handle structure and a sample discharge push handle structure; wherein the sample injection push handle structure is used to push the sample tube placed on the sample injection platform in the longitudinal direction for sample injection; the sample injection horizontal push handle structure is used to push the sample tube in the transverse direction for sample injection for detection; and the sample discharge push handle structure is used to push the detected sample tube to the sample discharge platform for sample discharge.

[0014] Preferably, the injection pusher structure has an injection pusher, and the injection pusher is arranged on a guide rail through a slider assembly; the slider assembly is fixed on a belt, and a motor drives the slider assembly to move back and forth longitudinally along the guide rail through a driving wheel, a driven wheel and the belt, thereby pushing the sample tube into the sample longitudinally for injection.

[0015] The injection push handle structure is used to push the sample tube placed on the injection platform in the longitudinal direction, and cooperate with the injection horizontal push handle structure to further push the sample tube in the transverse direction for injection; with the above structure, the slider assembly 1 is fixed on the belt 1, and the movement of the belt 1 drives the slider assembly 1 to reciprocate longitudinally along the guide rail 1, the structure is simple, and the response is fast; and with the guide rail 1 for guidance, the movement direction of the sample tube is stable and the error rate is low.

[0016] Preferably, the sample discharging pusher structure has a sample discharging pusher, and the sample discharging pusher is arranged on the guide rail three through the slider assembly three; the slider assembly three is fixed on the belt three, and the motor three drives the slider assembly three to move back and forth along the guide rail three longitudinally through the driving wheel three, the driven wheel three and the belt three, thereby pushing the tested sample tube to the sample discharging platform for sampling.

[0017] Preferably, the sample injection horizontal push handle structure has a plurality of horizontal push handles, and the plurality of horizontal push handles are arranged on the guide rail 2 through the slider assembly 2; the slider assembly 2 is fixed on the belt 2, and the motor 2 drives the slider assembly 2 to move back and forth laterally along the guide rail 2 through the driving wheel 2, the driven wheel 2 and the belt 2, thereby pushing the sample tube laterally into the sample injection for detection; the sample tube is placed on a test tube rack with multiple test tube rack positioning grooves, and the distance between two adjacent horizontal push handles is not greater than the length of the test tube rack.

[0018] In the present invention, the structures of the sample injection horizontal push dial, the reagent compartment dial, and the dilution cup dial are all the same, and they all have a dial head, an elastic member (generally a spring) is arranged below the dial head, and a dial tail part movably connected to the slider assembly, and the dial head is connected to the dial tail part through an inclined part; the above-mentioned horizontal dial movement is driven by the motor to drive the slider assembly, and then drive the horizontal dial movement, advancing one sample tube position of the test tube rack at a time, and then the horizontal dial hand moves backward, and the dial head is pressed down and moved backward due to the action of the bottom of the test tube rack, and then advanced one sample tube position under the action of the motor, and such repeated movements push the test tube rack forward; the distance between two adjacent horizontal dials is not greater than the length of the test tube rack, ensuring that there is always a horizontal dial at the bottom of the test tube rack to push.

[0019] Preferably, the sample injection and sample output device further comprises a transverse motion track, and the test tube rack performs sample injection and sample output along the transverse motion track.

[0020] The transverse motion track is set to make the sample injection and sample output of the test tube rack orderly and regular, optimizing the overall structure.

[0021] Preferably, the sample tube mixing assembly has a swing arm for mixing the sample, and the swing arm is connected to the rotating shaft of the swing motor to drive the sample tube to swing left and right to mix the sample inside the sample tube.

[0022] Preferably, the swing motor is connected to a horizontal slider assembly, and the horizontal slider assembly is driven by a horizontal motor to move horizontally along a horizontal guide rail; the horizontal motor is connected to upper and lower fixed blocks, and the upper and lower fixed blocks are connected to the upper and lower slider assemblies, and the upper and lower slider assemblies are arranged on upper and lower guide rails; the upper and lower fixed blocks are driven by the upper and lower motors to move up and down along the upper and lower guide rails.

[0023] The switching between horizontal and up and down directions enables the sample tube mixing assembly to accurately and quickly grab and lift the sample tube to prepare for swing mixing.

[0024] Preferably, the upper and lower motors and the horizontal motor are both screw motors; an upper and lower motion optical coupler is provided on the side of the upper and lower motors, and correspondingly, an upper and lower motion sensing piece is provided on the upper and lower slider assembly; a horizontal motion optical coupler is provided on the side of the horizontal motor, and correspondingly, a horizontal motion sensing piece is provided on the horizontal slider assembly.

[0025] The moving position of the screw motor is controllable and precise; the sensor piece cooperates with the optical coupler to control the up and down and horizontal movement distance of the swing arm; the optical coupler cooperates with the sensor piece, when the sensor piece moves to the optical coupler, the optical coupler senses the signal change, and the motion motor stops working.

[0026] Preferably, the swing arm is provided with a spring clip for clamping the sample tube for sample mixing; the spring clip is tensioned appropriately so as not to damage the sample tube or cause the sample tube to fall off during grabbing or mixing swinging.

[0027] Preferably, the sampling needle movement assembly includes a sampling needle up and down movement assembly and a sampling needle horizontal movement assembly; wherein, the sampling needle up and down movement assembly has a sampling needle up and down movement motor, the sampling needle is arranged on the sampling needle up and down movement slider assembly in the sampling needle movement assembly, and the sampling needle up and down movement motor drives the sampling needle up and down movement slider assembly to reciprocate along the sampling needle up and down guide rails; the sampling needle horizontal movement assembly has a sampling needle horizontal movement motor, which is connected to the sampling needle horizontal movement slider assembly through a fixed vertical plate, and the sampling needle horizontal movement motor drives the sampling needle horizontal movement slider assembly to reciprocate on the sampling needle horizontal guide rail.

[0028] The horizontal and vertical directions of the sampling needle are switched by the sampling needle up and down motion assembly and the sampling needle horizontal motion assembly, so that the sampling needle can accurately extend into the sample tube for sampling; the fixed vertical plate is used to install the various components in the sampling needle up and down motion assembly.

[0029] Preferably, the sampling needle up and down movement motor is a screw motor, and the sampling needle up and down movement slider assembly is mounted on the sampling needle up and down movement motor screw connected to the sampling needle up and down movement motor; the sampling needle horizontal movement motor is a stepping motor, and the sampling needle horizontal movement motor drives the sampling needle horizontal movement driving wheel to rotate, and the sampling needle horizontal movement driven wheel is driven by the sampling needle horizontal movement belt mounted on the sampling needle horizontal movement driving wheel, and the fixed vertical plate is connected to the sampling needle horizontal movement belt through the sampling needle horizontal movement slider assembly.

[0030] Preferably, the sampling needle motion assembly further comprises a guide bending belt, one end of which is fixed above the fixed vertical plate, and the other end is arranged on the sampling needle motion assembly support frame, and the guide bending belt is arranged longitudinally along the sampling needle horizontal guide rail.

[0031] The guide bending belt further stabilizes the sampling needle moving assembly, preventing the sampling needle from shaking laterally when moving horizontally, and ensuring accurate sampling; in addition, the inside of the guide bending belt is an electric circuit or liquid pipeline, so that the electric circuit or liquid pipeline is not exposed, and is neat and beautiful.

[0032] Preferably, a sampling needle up and down movement limiting optical coupler is provided on the fixed vertical plate and located at the upper end of the sampling needle upper and lower guide rails; a sampling needle horizontal movement limiting optical coupler is provided on the sampling needle motion component support frame and located at the end close to the sampling needle horizontal guide rail.

[0033] Preferably, the sampling needle is arranged on the sampling needle up and down moving slider assembly; and a dilution needle is also arranged on the fixed vertical plate.

[0034] The moving direction of the dilution needle is controlled by a set of sampling needle motion components, which has a simple structure, saves costs, is easy to programmatically control, and has a high degree of automation.

[0035] Preferably, the reagent chamber mechanism includes a reagent chamber bracket arranged on a base, on which a plurality of mutually independent reagent chambers are placed, and each reagent chamber can be stacked with a plurality of reagent cards; correspondingly, a plurality of mutually independent reagent chamber hand devices are arranged at the bottom of each reagent chamber and located in the reagent chamber bracket; the reagent chamber hand device is used to push the reagent card in the reagent chamber onto the reagent card detection track located in the bracket.

[0036] Preferably, a reagent card detector is installed on the inner wall of the reagent bin, and the reagent card detector is used to detect the number of reagent cards; a fixed block corresponding to each reagent bin is also provided on the reagent bin bracket; and a magnet is provided on the reagent bin or the fixed block.

[0037] The fixing block is used to fix the reagent chamber inserted into the reagent chamber bracket, and a magnet is provided on the reagent chamber or the fixing block to further ensure that the reagent chamber is inserted in place and accurately.

[0038] The reagent card detector is installed on the side wall of the reagent compartment, which can detect the number of reagent cards and remind you to add reagent cards in time; the magnet is to make the reagent compartment tightly combined with the fixed block when the reagent card is pulled out and then pushed in. The magnet can be a magnet at the bottom of the rear wall of the reagent compartment and the fixed block, or one side has a magnet and the other side is metal; the reagent compartment push-in sensor is installed on the fixed block to ensure that the reagent compartment is pushed into place; 20-30 reagent cards can be placed in a magazine, which can be placed according to the test items and configured according to the needs.

[0039] Preferably, the reagent bin dial device has a reagent bin dial motor, and the reagent bin dial motor drives a dial slider assembly fixedly connected to the dial belt to move back and forth along the dial guide rail through a dial driving wheel, a dial driven wheel and a dial belt; a reagent bin dial is provided on the dial slider assembly.

[0040] Preferably, the reagent bin dialer includes a dialer head, and an elastic member is arranged below the dialer head; the reagent bin dialer also has a dialer tail movably connected to the dialer slider assembly, and the dialer head is connected to the dialer tail through an inclined portion; an upper pressure plate is arranged on the reagent bin bracket.

[0041] After the reagent compartment dial moves to the reagent card detection track, it immediately moves backward to the initial position. Under the action of the spring (elastic part) and the upper pressure plate, the dial head is pressed down. When the dial continues to dial the reagent card to the detection track, the dial head pops up and pushes the reagent card to the reagent card detection track.

[0042] Preferably, the reagent compartment is also provided with a reagent compartment handle; and the fixing block is provided with a reagent compartment push-in-place sensor.

[0043] The reagent bin handle facilitates pushing and pulling the reagent bin. After the reagent bin is pulled out, the upper cover is opened and the reagent card is placed in. The reagent bin push-in position sensor detects whether the reagent bin is pushed in place, so as to cooperate with other components, especially the reagent bin pusher device, which needs to push the reagent card in the reagent bin to the reagent card detection track located in the bracket, and has strict requirements on the position of the reagent bin.

[0044] Preferably, the reagent compartment bracket is also provided with a plurality of mutually independent reagent compartment slots; the reagent compartment slots are connected and fixed above by a fixing plate.

[0045] The reagent tank is used to insert the reagent tank, and the reagent tank is connected and fixed above the tank by a fixing plate so that the reagent tank does not swing in the lateral direction.

[0046] Preferably, the optical path detection component is located in the bracket, and includes an optical path detection component fixing block, the optical path detection component fixing block is connected to the optical path detection component slider assembly, and the optical path detection component slider assembly is located on the optical path detection component translation guide rail; the optical path detection component also includes an optical path detection component translation motor, and the optical path detection component translation motor drives the optical path detection component fixing block to reciprocate along the optical path detection component translation guide rail; a light collecting head assembly is installed on the optical path detection component fixing block.

[0047] The optical path detection component fixing block reciprocates along the optical path detection component translation guide rail, so that the optical path detection component translates in the bracket, which facilitates the light-collecting head component to find the reagent card.

[0048] Preferably, a light-collecting head motion motor is also installed on the optical path detection component fixing block, the light-collecting head component is connected to the light-collecting head slider component, and the light-collecting head motion motor drives the light-collecting head component to reciprocate along a direction perpendicular to the translation guide rail of the optical path detection component.

[0049] With the above structure, the light collecting head assembly can reciprocate and scan the found reagent card, and move back and forth in the detection window of the reagent card to collect light signals.

[0050] Preferably, the light-collecting head assembly comprises an upper shielding cover and a lower light-collecting head; through a window provided on the fixing block of the optical path detection assembly, the light-collecting head assembly can move back and forth to collect light signals from the reagent card detection window located below.

[0051] Preferably, the optical path detection component fixing block is connected to one end of the detection component guide bending belt, and the detection component guide bending belt is arranged along the direction of the optical path detection component translation guide rail and is located on the side of the optical path detection component translation guide rail.

[0052] The guide bending belt of the detection component is used to keep the light head component stable in the horizontal direction and collect light signals accurately; in addition, the inside of the guide bending belt is an electric circuit or a liquid pipeline, so that the electric circuit or the liquid pipeline is not exposed and is neat and beautiful.

[0053] Preferably, the fully automatic fluorescent immunoassay analyzer also includes an emergency sample placement position, which is used to place a sample tube and is provided with an emergency position and an emergency sample tube detection sensor; the emergency sample placement position is located in the horizontal movement direction of the sampling needle.

[0054] The emergency position is used to start the emergency testing process; the emergency sample tube detection sensor is used to detect whether the emergency sample tube is placed in place before continuing the testing process; the emergency sample placement position is set in the horizontal movement direction of the sampling needle, so that the horizontally moving sampling needle can accurately extend into the emergency sample tube for sampling.

[0055] Preferably, the fully automatic fluorescent immunoassay analyzer is provided with a test tube rack detection sensor, a test tube rack positioner and a sample tube detection sensor on the rear side of the sample injection and sample output device; wherein the test tube rack detection sensor is installed on the vertical plate of the bracket, and the test tube rack positioner and the sample tube detection sensor are sensed through the corresponding windows of the vertical plate of the bracket.

[0056] The test tube rack detection sensor is used to detect whether the test tube rack is pushed into the predetermined position; the test tube rack positioner is used for positioning; and the sample tube detection sensor is used to detect whether the sample tube is in the predetermined position.

[0057] Preferably, a sample code scanner is arranged above the sample tube detection sensor; a code scanning rotation mechanism is arranged near the sample code scanner, and the code scanning rotation mechanism includes a sample tube rotating motor, and the sample tube rotating motor drives the rotating wheel to rotate; the code scanning rotation mechanism also includes a rotating wheel 1 and a rotating wheel 2, and the rotating wheel 1 and the rotating wheel 2 are both connected to a tensioning spring, and there is a gap between the rotating wheel 1 and the rotating wheel 2 and the rotating wheel.

[0058] Because the test tube rack is relatively light, a reagent rack locator and a locating groove are provided, and the two cooperate to enable the test tube rack to be pressed down and moved by a lever on the lateral motion track without being suspended; the sample tube rotating motor drives the rotating wheel to rotate, and the sample tube is stuck between the rotating wheel and two rotating wheels (each connected to a spring). Due to the rotation of the rotating wheel and the pressure of the two rotating wheels under the action of the springs, the sample tube is stuck and rotates slowly. When the sample tube rotates to the point where the barcode faces the sample scanner, the sample scanner scans the barcode, and the scanning rotating motor stops working, and the sample tube continues to be driven to move by the lever.

[0059] There is a gap between the first and second rotating wheels and the rotating wheel. After the rotating wheel is activated, the sample tube is rotated between the first and second rotating wheels and the rotating wheel, and is clamped by the tensioning spring and rotated until the sample scanner scans the code successfully.

[0060] Preferably, a reagent card scanner is provided in the bracket, and the reagent card scanner is located above the reagent card detection track; a sampling needle cleaning component is also provided in the bracket.

[0061] The reagent card scanner is used to scan the reagent card located on the reagent card detection track, obtain the reagent card information, and confirm that the sample detection item is correct; the sampling needle cleaning component is used to clean the sampling needle.

[0062] Preferably, a dilution cup compartment is further provided in the bracket, a plurality of dilution cups are placed in the dilution cup compartment, and a magnet is provided in the dilution cup compartment; a dilution cup hand is provided at the bottom of the dilution cup compartment, and a dilution cup hand motor drives a dilution cup hand driving wheel and a dilution cup hand driven wheel to drive the dilution cup hand to a predetermined dilution position; a dilution cup hand driving wheel and a dilution cup hand driven wheel are provided with a dilution cup hand belt, the dilution cup hand belt is connected to a dilution cup hand slider assembly, and the dilution cup hand is connected to the dilution cup hand slider assembly; correspondingly, a diluent bottle is also provided in the bracket.

[0063] The dilution needle can obtain the diluent from the diluent bottle through the pipeline and inject it into the dilution cup.

[0064] Preferably, a reagent card pusher for pushing the reagent card is provided on the reagent card detection track, and the reagent card pusher drives the reagent card pusher active wheel and the reagent card pusher driven wheel through the reagent card pusher motor to push the reagent card to the incubation detection position.

[0065] The incubation device at the incubation detection position in the present invention belongs to the prior art.

[0066] Preferably, the outside of the bracket is also covered with a shell, and a display screen is arranged on the shell; a disposal port and an instrument gripper are also arranged on the side of the shell; the fully automatic fluorescent immunoassay analyzer is also provided with a built-in printer, and the paper outlet of the built-in printer is arranged on the shell; the shell is also provided with an observation window and an item scanner.

[0067] The display screen is used to display content related to the test, and the analyzer can also be operated through the display screen (touch); the dilution cup is pushed to the disposal port, and the reagent card after the test is pushed to the disposal port; the instrument gripper is used to carry the instrument; the built-in printer is used to print the test results on paper; the project scanner function: scan and input the new test project to facilitate the subsequent reagent card scan information verification; update the reagent batch number, input the information details including the production validity period, monitor the effectiveness of the reagent use, etc.

[0068] Another problem to be solved by the present invention is to provide a sample detection method, the sample detection method includes a normal detection process, and the normal detection process includes the following steps:

[0069] (1) Turn on the power switch and wait for the instrument to initialize and be ready;

[0070] (2) After the project is imported, place the test tube rack with the sample tube, put in the reagent card, put in the diluent cup, and enter the patient information, sample information and items to be tested;

[0071] (3) Click the detection button to push the test tube rack to the horizontal motion track. After the test tube rack is detected to be in place, the test tube rack is pushed to the sample tube scanning position. After detecting that there is a sample tube in this hole of the test tube rack, the sample barcode scanner is turned on and the sample tube is slowly rotated so that the barcode on the sample tube faces the sample barcode scanner. The sample barcode scanner scans the barcode and uploads the data;

[0072] (4) After the sample information is verified to be correct, remove the reagent card from the reagent compartment of the corresponding test item, move the reagent card, scan the reagent card QR code at the reagent card scanning position, and upload the data;

[0073] (5) After the reagent card information is verified to be correct, push the reagent card to the sample loading position;

[0074] (6) Move the dilution liquid cup to the dilution position corresponding to the sample addition position;

[0075] (7) Move the test tube rack to the sample tube mixing position, fork the sample tube, lift the forked sample tube to a certain height, and swing the sample tube back and forth within a certain angle range to mix the sample in the test tube;

[0076] (8) After mixing is completed, insert the sample tube back into the test tube rack and move the test tube rack to the sampling position;

[0077] (9) Move the sampling needle to the top of the sample tube, lower the sampling needle so that the sampling needle pierces the sample tube, and extract the sample in the sample tube through the sampling needle;

[0078] (10) After the sample sampling is completed, the sampling needle is lifted and moved to the top of the dilution cup, the sample drawn from the sampling needle is injected into the dilution cup, and a certain proportion of diluent is injected from the dilution needle into the dilution cup;

[0079] (11) After the dilution is completed, move the sampling needle to the top of the dilution cup, lower the end of the sampling needle to the bottom of the dilution cup, and draw the diluted sample into the sampling needle;

[0080] (12) lifting the sampling needle, moving the sampling needle to the sample loading position, lowering the sampling needle to above the sample loading port of the reagent card on the sample loading position, and injecting the sample in the sampling needle into the sample loading port of the reagent card;

[0081] (13) Push the reagent card after sample loading to the incubation detection position, start the heating module, and keep the reagent card incubated at the set temperature;

[0082] (14) After the incubation is completed, the detection component is moved to the top of the reagent card, the data acquisition module is started, the light collecting head is moved in the detection window of the reagent card, and the data acquisition module obtains the scanning signal;

[0083] (15) Move the test tube rack to the sample dispensing position, push the test tube rack to the sample dispensing platform, push the completed dilution cup to the disposal port, and push the completed reagent card to the disposal port;

[0084] (16) After the test procedure is completed, the test results can be viewed on the instrument display and printed using the built-in printer.

[0085] Preferably, an emergency detection process is also included, and the emergency detection process includes the following steps:

[0086] (1) Turn on the power switch and wait for the instrument to initialize and be ready;

[0087] (2) After the project is imported, place the test tube rack with the sample tube, put in the reagent card, put in the diluent cup, and enter the patient information, sample information and items to be tested;

[0088] (3) Open the emergency sample placement position, insert the emergency test tube into the emergency sample test tube slot, push the emergency sample placement position back, click the emergency button, and start the test process after detecting that the emergency sample has been inserted; then follow the normal test process steps (4)-(14), (16);

[0089] Alternatively, during the normal testing process, execute step (3) in the above emergency testing process. BRIEF DESCRIPTION OF THE DRAWINGS

[0090] The following is further described in detail with reference to the accompanying drawings and embodiments of the present invention:

[0091] Figure 1 It is a schematic diagram of the overall structure of the fully automatic fluorescent immunoassay analyzer of the present invention;

[0092] Figure 2 yes Figure 1 Schematic diagram of the internal structure of the fully automatic fluorescent immunoassay analyzer;

[0093] Figure 3 yes Figure 2 A schematic diagram of the structure of the sample injection and sample output device partially shown in FIG.

[0094] Figure 4 yes Figure 3 Schematic diagram of the structure of the sample introduction and sample discharging device;

[0095] Figure 5 yes Figure 3 Schematic diagram of the structure of the sample introduction and sample discharging device;

[0096] Figure 6 yes Figure 3 Schematic diagram of the sample discharging push handle structure of the sample discharging device;

[0097] Figure 7 It is a diagram of the test tube rack structure;

[0098] Figure 8 It is a schematic diagram of the structure of the sample tube mixing assembly of the present invention;

[0099] Fig. 9 yes Figure 8 Schematic diagram of the structure of the sample tube mixing assembly when it is placed flat;

[0100] Fig.10 It is a schematic diagram of the structure of the sampling needle motion assembly of the present invention;

[0101] Fig.11 yes Fig.10 A schematic diagram of the rear view structure of the sampling needle motion assembly;

[0102] Fig.12 It is a schematic diagram of the structure of the reagent compartment mechanism of the present invention;

[0103] Fig.13 yes Fig.12 A schematic diagram of the partial cross-sectional structure of the middle reagent chamber mechanism;

[0104] Fig.14 yes Fig.12 A schematic diagram of the structure of a single reagent compartment in the reagent compartment mechanism;

[0105] Fig.15 It is a schematic diagram of the structure of the optical path detection component of the present invention;

[0106] Fig.16 yes Fig.15 Schematic diagram of the local structure of the optical path detection component;

[0107] Fig.17 It is a schematic diagram of the structure of the sample injection and sample output platform;

[0108] Fig.18 It is a position diagram of the sample injection horizontal push handle structure in the fully automatic fluorescent immunoassay analyzer of the present invention;

[0109] Fig.19 This is a schematic diagram of the structure of the code scanning and rotating mechanism;

[0110] Fig. 20 yes Fig.19 Schematic diagram of the top view of the central scanning code rotating mechanism;

[0111] Fig.21 It is a schematic diagram of the position structure of the test tube rack detection sensor, the test tube rack positioner and the sample scanner;

[0112] Fig. 22 yes Figure 2 Schematic diagram of the local structure;

[0113] Fig.23 yes Figure 2 A schematic diagram of the partially enlarged structure;

[0114] Fig.24 It is a structural schematic diagram of a reagent bin handle in a reagent bin mechanism;

[0115] Fig.25 yes Fig.24 A schematic diagram of the structure when the reagent compartment handle is in the initial position;

[0116] Fig.26 yes Figure 2 The structural schematic diagram of the cleaning pool is shown in the partial enlargement in the middle;

[0117] Fig. 27 yes Figure 2 The middle part is enlarged to show the schematic diagram of the cleaning pool from top view;

[0118] Fig.28 yes Figure 2 The middle part is enlarged to show the schematic diagram of the structure of the reagent card handle and the dilution cup handle;

[0119] Fig.29 yes Figure 2 A schematic diagram of the partially enlarged structure of the middle side;

[0120] Fig.30 It is a schematic diagram of the dilution cup structure;

[0121] Fig.31 It is a partial upward structural schematic diagram of the bottom of the fully automatic fluorescent immunoassay analyzer of the present invention;

[0122] Fig.32 It is a schematic diagram of the structure of the incubation device installation position;

[0123] Fig.33 It is a schematic diagram of the top view of the structure of the fully automatic fluorescent immunoassay analyzer of the present invention;

[0124] Fig.34 It is a schematic diagram of the structure of the horizontal push dial hand in motion state;

[0125] Among them: 1- bracket, 101- vertical plate, 2- base, 3- sample injection and sample discharging device, 301- sample injection push handle structure, 3011- sample injection push handle, 3012- slider component one, 3013- guide rail one, 3014- belt one, 3015- motor one, 3016- driving wheel one, 3017- driven wheel one, 302- sample injection horizontal push handle structure, 3021- horizontal push handle, 3022- slider component two, 3023- guide rail two, 3024- belt two, 3025- motor two, 3026- driving wheel two, 3027- driven wheel two, 303- sample discharging push handle structure, 3031- sample discharging push handle, 3032- slider component three, 3033- guide rail three, 3034- belt three, 3035- motor three, 3036-driving wheel three, 3037-driven wheel three, 304-injection platform, 305-lateral motion track, 306-sample platform, 4-sample tube, 5-sample tube mixing assembly, 501-swing arm, 502-swing motor, 503-horizontal slider assembly, 504-horizontal motor, 505-horizontal guide rail, 506-upper and lower fixed blocks, 507-upper and lower slider assembly, 508-upper and lower guide rails, 509-upper and lower motor, 5010-upper and lower motion optical coupler, 5011-upper and lower motion sensor sheet, 5012-horizontal motion optical coupler, 5013-horizontal motion sensor sheet, 5014-shrapnel clip, 6-sampling needle motion assembly, 601-sampling needle, 602-sampling needle up and down motion assembly, 6021-sampling needle up and down Motion motor, 6022-sampling needle up and down motion slider assembly, 6023-sampling needle up and down guide rails, 6024-sampling needle up and down motion motor lead screw, 6025-sampling needle up and down motion limit optical coupler, 603-sampling needle horizontal motion assembly, 6031-sampling needle horizontal motion motor, 6032-sampling needle horizontal motion slider assembly, 6033-sampling needle horizontal guide rail, 6034-sampling needle horizontal motion driving wheel, 6035-sampling needle horizontal motion belt, 6036-sampling needle horizontal motion driven wheel, 6037-sampling needle horizontal motion limit optical coupler, 604-fixed vertical plate, 605-guide bending belt, 606-sampling needle motion assembly support frame, 607-dilution needle, 7-reagent bin mechanism, 701-reagent card , 702-reagent bin bracket, 7021-upper pressure plate, 7022-reagent bin slot, 7023-fixed plate, 703-reagent bin, 7031-reagent bin handle, 704-reagent bin hand device, 7041-reagent bin hand motor, 7042-hand driving wheel, 7043-hand driven wheel, 7044-hand belt, 7045-hand slider assembly, 7046-hand guide rail, 7047-reagent bin hand, 7048-hand head, 7049-elastic part, 70410 hand tail, 70411-inclined part, 705-reagent card detection track, 706-reagent card detector, 708-fixed block, 709-magnet, 7010-reagent bin push position sensor, 8-optical path detection assembly,801-optical path detection component fixing block, 8011-window, 802-optical path detection component slider assembly, 803-optical path detection component translation guide rail, 804-optical path detection component translation motor, 805-lighting head assembly, 8051-shielding cover, 8052-lighting head, 806-lighting head motion motor, 807-lighting head slider assembly, 808-detection component guide bending belt, 9-test tube rack, 901-test tube rack positioning groove, 10-emergency sample placement position, 11-emergency position, 12-emergency sample tube detection sensor, 13-test tube rack detection sensor, 14-test tube rack positioner, 15-sample tube detection sensor, 16-sample code scanner, 17-code scanning rotation mechanism, 1701-sample tube rotation motor, 1702-rotating wheel, 1703-rotating wheel one, 1704-rotating wheel two, 1 705-tension spring, 18-reagent card scanner, 19-sampling needle cleaning assembly, 20-dilution cup compartment, 2001-dilution cup, 2002-dilution cup hand, 2003-dilution cup hand motor, 2004-dilution cup hand driving wheel, 2005-dilution cup hand driven wheel, 2006-dilution cup hand belt, 2007-dilution cup hand slider assembly, 2008-dilution liquid bottle, 21-reagent card hand, 22-reagent card hand motor, 23-reagent card hand driving wheel, 24-reagent card hand driven wheel, 25-housing, 26-display, 27-disposal port, 28-instrument gripper, 29-built-in printer, 2901-paper outlet, 30-observation window, 31-project scanner, 32-emergency sample sampling port, 33-normal sample sampling port, 34-incubation detection position. , DETAILED DESCRIPTION

[0126] like Figure 1 , 2 As shown, the fully automatic fluorescent immunoassay analyzer of this embodiment includes a bracket 1, which is arranged on a base 2, and a sample injection and sample discharge device 3 is arranged on the base 2, and the sample injection and sample discharge device 3 is used to push a sample tube 4 into the sample for detection and push the sample tube 4 out for sample discharge; a sample tube mixing component 5 is arranged on the side of the sample injection and sample discharge device 3, and the sample tube mixing component 5 is used to mix the sample in the sample tube 4, wherein the sample tube 4 is pushed into the sample injection for detection and pushed out for sample discharge by the sample injection and sample discharge device 3; and also includes a sampling needle motion component 6 for extracting the sample in the sample tube 4, and a sampling needle 601 is installed on the sampling needle motion component 6, as shown in FIG. Fig.10 As shown; a reagent compartment mechanism 7 is provided in the bracket 2, and a plurality of reagent cards 701 are stacked in the reagent compartment mechanism 7, as shown Fig.14 As shown, the sampling needle 601 adds the diluted sample to the sample adding port of the reagent card 701 ; the bracket 2 is also provided with an optical path detection component 8 for detecting the reagent card 701 .

[0127] The outside of the bracket 1 is also covered with a shell 25, and a display screen 26 is provided on the shell 25, and all the contents related to the test can be viewed on the display screen 26; a disposal port 27 and an instrument gripper 28 are also provided on the side of the shell 25; the fully automatic fluorescent immunoassay analyzer is also provided with a built-in printer 29, and a paper outlet 2901 of the built-in printer 29 is provided on the shell 25; an observation window 30 and an item scanner 31 are also provided on the shell 25; a reagent card scanner 18 (such as Fig.23 As shown), the reagent card scanner 18 is located above the reagent card detection track 705; a sampling needle cleaning component 19 is also provided in the bracket 1. When the sampling needle cleaning component 19 cleans the sampling needle 601, the inner wall of the needle is rinsed by entering the sampling needle 601 through the cleaning system, and the outer wall of the needle is rinsed by entering the cleaning liquid through the side inlet, and the waste liquid is discharged from the lower discharge port through the drainage system; a normal sample sampling port 33 and an emergency sample sampling port 32 are provided on the partition connected to the sampling needle cleaning component 19. Because the sample tube cover is made of rubber, the tube cap is prevented from being pulled out when the sampling needle 601 is inserted into the sample and then pulled out, as shown in FIG. Fig. 27 shown.

[0128] like Figure 3-6 As shown, the sample feeding and discharging device 3 includes a sample feeding push handle structure 301, a sample feeding horizontal push handle structure 302 and a sample discharging push handle structure 303, and also includes a horizontal motion track 305 (such as Fig.17 As shown); wherein, the sample introduction push handle structure 301 is used to push the sample tube 4 placed on the sample introduction platform 304 into the sample introduction along the longitudinal direction; the sample introduction horizontal push handle structure 302 is used to push the sample tube 4 into the sample introduction along the transverse direction for testing; the sample discharge push handle structure 303 is used to push the sample tube 4 after testing to the sample discharge platform 306 for discharging, as shown in FIG. Fig.17 shown.

[0129] Specifically, Figure 4 As shown, the sample injection handle structure 301 has a sample injection handle 3011, and the sample injection handle 3011 is set on a guide rail 3013 through a slider assembly 3012; the slider assembly 3012 is fixed on a belt 3014, and a motor 3015 drives the slider assembly 3012 to move back and forth longitudinally along the guide rail 3013 through a driving wheel 3016, a driven wheel 3017 and the belt 3014, and switches, thereby pushing the sample tube 4 (located on the test tube rack 9) in the longitudinal direction for injection.

[0130] The sample tube 4 (located on the test tube rack 9) is pushed into the horizontal motion track 305 by the sample pusher 3011, and the test tube rack 9 performs sample injection and sample removal along the horizontal motion track 305. Figure 5As shown, the sample injection horizontal push dial structure 302 has a plurality of horizontal dial hands 3021, and there are three in this embodiment. It should be noted that the dial hand structures involved in this embodiment are all the same, including a dial hand head, a dial hand tail, and an inclined portion. When the horizontal dial hand 3021 pushes the test tube rack 9 horizontally, the dial hand head faces the pushing direction. In this way, when the horizontal dial hand 3021 moves back, the dial hand head is pressed by the test tube rack 9, and the bottom surface of the reagent rack 9 is concave, so the dial hand head 7048 can bounce up again after being pressed down to push the reagent rack 9 to move, as shown in FIG. Fig.18 , 24 , 34; the horizontal hand 3021 is set on the guide rail 3023 through the slider assembly 3022; the slider assembly 3022 is fixed on the belt 3024, and the motor 3025 drives the slider assembly 3022 to move back and forth along the guide rail 3023 through the driving wheel 3026, the driven wheel 3027 and the belt 3024, so as to push the sample tube 4 on the test tube rack 9 into the sample for testing; the sample tube 4 is placed on the test tube rack 9 with multiple test tube rack positioning grooves 901, as shown in FIG. Figure 7 As shown, the test tube rack 9 indicates 10 sample tube positions, which is a preferred number; the distance between two adjacent horizontal dialing hands 3021 is not greater than the length of the test tube rack 9.

[0131] like Figure 6 As shown, the sample discharging handle structure 303 has a sample discharging handle 3031, and the sample discharging handle 3031 is arranged on the guide rail 3033 through the slider assembly 3032; the slider assembly 3032 is fixed on the belt 3034, and the motor 3035 drives the slider assembly 3032 to move back and forth along the guide rail 3033 longitudinally through the driving wheel 3036, the driven wheel 3037 and the belt 3034, so as to push the sample tube 4 after detection to the sample discharging platform 306 for discharging, as shown in FIG. Fig.17 shown.

[0132] like Figure 8 , 9 As shown, the sample tube mixing assembly 5 has a swing arm 501 for swinging the sample tube 5, and the swing arm 501 is connected to the rotating shaft of the swing motor 502 to drive the sample tube 4 to swing left and right to mix the sample inside the sample tube 4, and the swing motor 502 is connected to the horizontal slider assembly 503, and the horizontal slider assembly 503 is driven by the horizontal motor 504 to move horizontally along the horizontal guide rail 505; the horizontal motor 504 is connected to the upper and lower fixed blocks 506, and the upper and lower fixed blocks 506 are connected to the upper and lower slider assembly 507, and the upper and lower slider assembly 507 is set on the upper and lower guide rails 508; the upper and lower fixed blocks 506 are driven by the upper and lower motors 509 to move up and down along the upper and lower guide rails 508.

[0133] In this embodiment, the up and down motors 509 and the horizontal motor 504 are both screw motors; an up and down motion optical coupler 5010 is provided on the side of the up and down motor 509, and correspondingly, an up and down motion sensing piece 5011 is provided on the up and down slider assembly 507; a horizontal motion optical coupler 5012 is provided on the side of the horizontal motor 504, and correspondingly, a horizontal motion sensing piece 5013 is provided on the horizontal slider assembly 503, and a spring clip 5014 is provided on the swing arm 501 for clamping the sample tube 4 for sample mixing.

[0134] The sampling needle motion assembly 6 includes a sampling needle up-and-down motion assembly 602 and a sampling needle horizontal motion assembly 603, respectively. Fig.10 , 11 The sampling needle up and down motion assembly 602 has a sampling needle up and down motion motor 6021, and the sampling needle 601 is arranged on the sampling needle up and down motion slider assembly 6022 in the sampling needle motion assembly 6, and the sampling needle up and down motion motor 6021 drives the sampling needle up and down motion slider assembly 6022 to reciprocate along the sampling needle up and down guide rail 6023; the sampling needle horizontal motion assembly 603 has a sampling needle horizontal motion motor 6031, and the sampling needle up and down motion assembly 602 is connected with the sampling needle horizontal motion slider assembly 6032 through a fixed vertical plate 604, and the sampling needle horizontal motion motor 6031 drives the sampling needle horizontal motion slider assembly 6032 to reciprocate on the sampling needle horizontal guide rail 6033.

[0135] In this embodiment, the sampling needle up and down motion motor 6021 is a screw motor, and the sampling needle up and down motion slider assembly 6022 is mounted on the sampling needle up and down motion motor screw 6024 connected to the sampling needle up and down motion motor 6021; the sampling needle horizontal motion motor 6031 is a stepping motor, and the sampling needle horizontal motion motor 6031 drives the sampling needle horizontal motion driving wheel 6034 to rotate, and drives the sampling needle horizontal motion driven wheel 6036 through the sampling needle horizontal motion belt 6035 mounted on the sampling needle horizontal motion driving wheel 6034, and the fixed vertical plate 604 is connected to the sampling needle horizontal motion belt 6035 through the sampling needle horizontal motion slider assembly 6032.

[0136] The sampling needle motion assembly 6 also includes a guide bending belt 605 , one end of which is fixed above the fixed vertical plate 604 , and the other end is arranged on the sampling needle motion assembly support frame 606 , and the guide bending belt 605 is longitudinally arranged along the sampling needle horizontal guide rail 6033 .

[0137] A sampling needle vertical motion limiting optical coupler 6025 is provided on the fixed vertical plate 604 and located at the upper end of the sampling needle vertical guide rail 6023 ; a sampling needle horizontal motion limiting optical coupler 6037 is provided on the sampling needle motion component support frame 606 and located at the end close to the sampling needle horizontal guide rail 6033 .

[0138] The sampling needle 601 is arranged on the sampling needle up-and-down moving slider assembly 6022 ; a dilution needle 607 is also arranged on the fixed vertical plate 604 .

[0139] like Figure 12-14 As shown, the reagent chamber mechanism 7 includes a reagent chamber support 702 arranged on the base 2, and a plurality of mutually independent reagent chambers 703 are placed on the reagent chamber support 702, and a plurality of reagent cards 701 can be stacked in each reagent chamber 703; correspondingly, a plurality of mutually independent reagent chamber hand devices 704 are arranged at the bottom of each reagent chamber 703 and located in the reagent chamber support 702; the reagent chamber hand device 704 is used to push the reagent card 701 in the reagent chamber 703 to the reagent card detection track 705 located in the support 1, as shown in FIG. Fig.23 , 28 and 33.

[0140] exist Fig.13 In the figure, a reagent card detector 706 is installed on the inner wall of the reagent compartment 703, and the reagent card detector 706 is used to detect the number of reagent cards 701 in the reagent compartment 703; a fixed block 708 corresponding to each reagent compartment 701 is also arranged on the reagent compartment bracket 702; and a magnet 709 is arranged on the reagent compartment 703 or the fixed block 708.

[0141] The reagent bin hand shifter device 704 has a reagent bin hand shifter motor 7041, which drives a hand shifter slider assembly 7045 fixedly connected to the hand shifter belt 7044 to reciprocate along a hand shifter guide rail 7046 through a hand shifter driving wheel 7042, a hand shifter driven wheel 7043 and a hand shifter belt 7044; a reagent bin hand shifter 7047 is provided on the hand shifter slider assembly 7045.

[0142] The reagent compartment dial 7047 includes a dial head 7048, such as Fig.24 , 25As shown, an elastic member 7049 is arranged below the dial hand head 7048, and a common spring is used in this embodiment; the reagent compartment dial hand 7047 also has a dial hand tail 70410 movably connected to the dial hand slider assembly 7045, and the dial hand head 7048 is connected to the dial hand tail 70410 through an inclined portion 70411; an upper pressing plate 7021 is arranged on the reagent compartment bracket 702; after the reagent compartment dial hand 7047 pushes out the reagent card 701, it moves backward, and due to the pressure of the reagent card 701, the dial hand head 7048 is pressed downward. Fig.25 The middle initial position is the position when the reagent compartment dial handle 7047 is reset, which does not affect the pushing and pulling of the reagent compartment 703. When work is required, the reagent compartment dial handle 7047 moves forward, and when it moves out of the position of the upper pressure plate 7021, the dial handle head 7048 pops out to push the reagent card 701 to move.

[0143] The reagent bin 703 is also provided with a reagent bin handle 7031 ; the fixing block 708 is provided with a reagent bin push-in position sensor 7010 ; the reagent bin support 702 is also provided with a plurality of mutually independent reagent bin slots 7022 ; the reagent bin slots 7022 are connected and fixed above by a fixing plate 7023 .

[0144] like Fig.15 , 16 As shown, the optical path detection component 8 is located in the bracket 1, and includes an optical path detection component fixing block 801, and the optical path detection component fixing block 801 is connected to the optical path detection component slider component 802, and the optical path detection component slider component 802 is located on the optical path detection component translation guide rail 803; the optical path detection component 8 also includes an optical path detection component translation motor 804, and the optical path detection component translation motor 804 drives the optical path detection component fixing block 801 to reciprocate along the optical path detection component translation guide rail 803; the optical path detection component fixing block 801 is installed with a light collecting head component 805.

[0145] A light-collecting head motion motor 806 is also installed on the optical path detection component fixing block 801. The light-collecting head component 805 is connected to the light-collecting head slider component 807. The light-collecting head motion motor 806 drives the light-collecting head component 805 to reciprocate in a direction perpendicular to the optical path detection component translation guide rail 803.

[0146] The skylight head assembly 805 includes an upper shielding cover 8051 and a lower skylight head 8052. Fig.16 As shown; through the window 8011 set on the optical path detection component fixed block 801, the light head component 805 can move back and forth to collect light signals from the detection window of the reagent card 701 located below.

[0147] The optical path detection component fixing block 801 is connected to one end of the detection component guide bending belt 808 . The detection component guide bending belt 808 is arranged along the direction of the optical path detection component translation guide rail 803 and is located on the side of the optical path detection component translation guide rail 803 .

[0148] The fully automatic fluorescent immunoassay analyzer of this embodiment also includes an emergency sample placement position, wherein the emergency sample placement position 10 is used to place a sample tube 4. Figure 3 and is provided with an emergency position 11 and an emergency sample tube detection sensor 12 (such as Fig.26 , 27 As shown); the emergency sample placement position 10 is located in the horizontal movement direction of the sampling needle 601.

[0149] like Fig.19 As shown, the fully automatic fluorescent immunoassay analyzer is provided with a test tube rack detection sensor 13, a test tube rack positioner 14 and a sample tube detection sensor 15 next to the sample injection and sample output device 3; wherein the test tube rack detection sensor 13 is installed on the vertical plate 101 of the bracket 1, and the test tube rack positioner 14 and the sample tube detection sensor 15 are sensed through the corresponding windows of the vertical plate 101 of the bracket 1.

[0150] A sample scanner 16 is disposed above the sample tube detection sensor 15. Fig. 20 , 21 As shown; a scanning rotating mechanism 17 (such as Fig. 22 As shown), the code scanning rotating mechanism 17 includes a sample tube rotating motor 1701, and the sample tube rotating motor 1701 drives the rotating wheel 1702 to rotate; the code scanning rotating mechanism 17 also includes a rotating wheel 1703 and a rotating wheel 2 1704, and the rotating wheel 1 1703 and the rotating wheel 2 1704 are both connected with a tensioning spring 1705, and there is a gap between the rotating wheel 1703 and the rotating wheel 2 1704 and the rotating wheel 1702.

[0151] A dilution cup chamber 20 is also provided in the support 1. Fig.28 , 29 As shown in FIG. 31 , a plurality of dilution cups 2001 (such as Fig.30 As shown), one sample test requires one cup position. Fig.30There are 8 cup positions, preferably 10, which are the same as the sample tube positions of the reagent rack 9; a dilution cup hand 2002 is arranged at the bottom of the dilution cup compartment 20, and the dilution cup hand motor 2003 drives the dilution cup hand driving wheel 2004 and the dilution cup hand driven wheel 2005 to drive the dilution cup hand 2002 to move the dilution cup 2001 to the dilution position; the dilution cup hand driving wheel 2004 and the dilution cup hand driven wheel 2005 are provided with a dilution cup hand belt 2006, and the dilution cup hand belt 2006 is connected to the dilution cup hand slider assembly 2007, and the dilution cup hand 2002 is connected to the dilution cup hand slider assembly 2007; correspondingly, a diluent bottle 2008 is also arranged in the bracket 1.

[0152] A reagent card hand 21 for transferring the reagent card 701 is arranged on the reagent card detection track 705. The reagent card hand 21 drives the reagent card hand driving wheel 23 and the reagent card hand driven wheel 24 through the reagent card hand motor 22, thereby driving the reagent card hand 21 to transfer the reagent card 701 to the incubation detection position 34. Fig.32 As shown, the incubation device is below the reagent card detection platform within the movement range of the optical path detection component 8. According to different projects, the reagent card 701 is incubated for different times. The light collecting head 8052 detects the light signal, and the data processing module collects, converts (light signal-electrical signal), amplifies and processes the light signal to obtain the detection result.

[0153] The sample detection method of the present invention includes a normal detection process, and the normal detection process includes the following steps:

[0154] (1) Turn on the power switch and wait for the instrument to initialize and be ready;

[0155] (2) After the project is imported, place the test tube rack with the sample tube, put in the reagent card, put in the diluent cup, and enter the patient information, sample information and items to be tested;

[0156] (3) Click the detection button to push the test tube rack to the horizontal motion track. After the test tube rack is detected to be in place, the test tube rack is pushed to the sample tube scanning position. After detecting that there is a sample tube in this hole of the test tube rack, the sample barcode scanner is turned on and the sample tube is slowly rotated so that the barcode on the sample tube faces the sample barcode scanner. The sample barcode scanner scans the barcode and uploads the data;

[0157] (4) After the sample information is verified to be correct, remove the reagent card from the reagent compartment of the corresponding test item, move the reagent card, scan the reagent card QR code at the reagent card scanning position, and upload the data;

[0158] (5) After the reagent card information is verified to be correct, push the reagent card to the sample loading position;

[0159] (6) Move the dilution liquid cup to the dilution position corresponding to the sample addition position;

[0160] (7) Move the test tube rack to the sample tube mixing position, fork the sample tube, lift the forked sample tube to a certain height, and swing the sample tube back and forth within a certain angle range to mix the sample in the test tube;

[0161] (8) After mixing is completed, insert the sample tube back into the test tube rack and move the test tube rack to the sampling position;

[0162] (9) Move the sampling needle to the top of the sample tube, lower the sampling needle so that the sampling needle pierces the sample tube, and extract the sample in the sample tube through the sampling needle;

[0163] (10) After the sample sampling is completed, the sampling needle is lifted and moved to the top of the dilution cup, the sample drawn from the sampling needle is injected into the dilution cup, and a certain proportion of diluent is injected from the dilution needle into the dilution cup;

[0164] (11) After the dilution is completed, move the sampling needle to the top of the dilution cup, lower the end of the sampling needle to the bottom of the dilution cup, and draw the diluted sample into the sampling needle;

[0165] (12) lifting the sampling needle, moving the sampling needle to the sample loading position, lowering the sampling needle to above the sample loading port of the reagent card on the sample loading position, and injecting the sample in the sampling needle into the sample loading port of the reagent card;

[0166] (13) Push the reagent card after sample loading to the incubation detection position, start the heating module, and keep the reagent card incubated at the set temperature;

[0167] (14) After the incubation is completed, the detection component is moved to the top of the reagent card, the data acquisition module is started, the light collecting head is moved in the detection window of the reagent card, and the data acquisition module obtains the scanning signal;

[0168] (15) Move the test tube rack to the sample dispensing position, push the test tube rack to the sample dispensing platform, push the completed dilution cup to the disposal port, and push the completed reagent card to the disposal port;

[0169] (16) After the test procedure is completed, the test results can be viewed on the instrument display and printed using the built-in printer.

[0170] Also included is an emergency testing process, which includes the following steps:

[0171] (1) Turn on the power switch and wait for the instrument to initialize and be ready;

[0172] (2) After the project is imported, place the test tube rack with the sample tube, put in the reagent card, put in the diluent cup, and enter the patient information, sample information and items to be tested;

[0173] (3) Open the emergency sample placement position, insert the emergency test tube into the emergency sample test tube slot, push the emergency sample placement position back, click the emergency button, and start the test process after detecting that the emergency sample has been inserted; then follow the normal test process steps (4)-(14), (16);

[0174] Alternatively, during the normal testing process, execute step (3) in the above emergency testing process.

[0175] Specifically, the steps of using the fully automatic fluorescent immunoassay analyzer in this embodiment to perform sample testing are:

[0176] Normal testing process: (1) Turn on the power switch and wait for the instrument to initialize and be ready;

[0177] (2) After the project is imported, a test tube rack 9 with a sample tube 4 is placed on the sample injection platform 304, a reagent card 701 is placed in the reagent compartment 703, a dilution cup 2001 is placed in the diluent compartment 20, and patient information, sample information, and items to be tested are entered;

[0178] (3) Click the detection button, the sample pusher pushes the test tube rack 9 to the horizontal motion track 305, and after the test tube rack detection sensor 13 detects that the test tube rack 9 is in place, the sample horizontal pusher 3021 pushes the test tube rack 9 to the sample tube scanning position. After the sample tube detection sensor 15 detects that there is a sample tube 4 in this hole of the test tube rack 9, the sample scanner 16 is turned on, and at the same time, the sample tube rotation motor 1701 is started to slowly rotate the sample tube 4 so that the barcode on the sample tube 4 faces the sample scanner 16. After the sample scanner 16 scans the barcode, it is uploaded to the host computer, and the data is uploaded;

[0179] (4) After the sample information is verified, the reagent compartment hand 7047 pulls the reagent card 701 from the reagent compartment 703 corresponding to the test item to the reagent card detection track 705, and the reagent card hand 21 moves the reagent card 701, and uses the reagent card scanner 18 to scan the two-dimensional code of the reagent card 701 at the reagent card scanning position, and uploads it to the host computer, and the data is long-distance transmitted;

[0180] (5) After the reagent card information is verified to be correct, the reagent card pusher 21 pushes the reagent card 701 to the sample loading position;

[0181] (6) The dilution cup moving hand 2002 moves the dilution cup 2001 from the dilution cup compartment 20 to the dilution position corresponding to the sample adding position;

[0182] (7) The sample injection horizontal lever 3021 moves the test tube rack 9 to the sample tube mixing position (sample tube mixing assembly 5), the mixing horizontal motion motor (i.e., the horizontal motor 504) pushes the test tube spring clip 5014 forward to fork the sample tube 4, the mixing up and down motion motor (i.e., the up and down motor 509) raises the spring clip 5014 forking the sample tube 4 to a certain height, and the mixing swing motor 502 makes the sample tube spring clip 5014 swing back and forth within a certain angle range to mix the sample in the sample tube 4;

[0183] (8) After the mixing is completed, the mixing swing motor 502 is reset, the mixing up-and-down motion motor 509 lowers the sample tube spring clip 5014, so that the sample tube 4 is inserted back into the test tube rack 9, the mixing horizontal motion motor 504 retracts the sample tube spring clip 5014, and the sample injection horizontal lever 3021 moves the test tube rack 9 to the sampling position;

[0184] (9) The sampling needle horizontal motion motor 6031 moves the sampling needle 601 to the top of the sample tube 4, and the sampling needle lifting motor (i.e., the sampling needle up and down motion motor 6021) lowers the sampling needle 601 so that the sampling needle 601 is inserted into the sample tube 4. The sampling pump is connected to the sampling needle 601 through a pipeline to extract the sample in the sample tube 4;

[0185] (10) After the sample sampling is completed, the sampling needle lifting motor 6021 lifts the sampling needle 601, and the sampling needle horizontal movement motor 6031 moves the sampling needle 601 to the top of the dilution cup 2001. The sampling pump pumps the sample drawn from the sampling needle 601 into the dilution cup 2001, and the dilution pump pumps a certain proportion of diluent from the dilution needle 607 into the dilution cup 2001 through the pipeline;

[0186] (11) After the dilution is completed, the sampling needle horizontal motion motor 6031 moves the sampling needle 601 to the top of the dilution cup 2001, and the sampling needle lifting motor 6021 lowers the end of the sampling needle 601 to the bottom of the dilution cup 2001. The sampling pump draws the diluted sample into the sampling needle 601 through the pipeline;

[0187] (12) The sampling needle lifting motor 6021 lifts the sampling needle 601, and the sampling needle horizontal motion motor 6031 moves the sampling needle 601 to the sample loading position. The sampling needle lifting motor 6021 lowers the sampling needle 601 to above the sample loading port of the reagent card on the sample loading position, and the sample in the sampling needle 601 is injected into the sample loading port of the reagent card 701;

[0188] (13) The reagent card pusher 21 pushes the reagent card 701 after sample loading to the incubation detection position, and the heating module in the incubation device is started to keep the reagent card 701 incubated at the set temperature;

[0189] (14) After the incubation is completed, the detection translation motor (i.e., the optical path detection component translation motor 804) moves the detection component (i.e., the optical path detection component 8) to the top of the reagent card 701, the data acquisition module is started, the light head motion motor 806 moves the light head 8052 in the reagent card detection window, and the data acquisition module obtains the scanning signal;

[0190] (15) The sample introduction horizontal pusher 3021 pushes the test tube rack 9 to the sample discharging position, the sample discharging pusher 3031 pushes the test tube rack 9 to the sample discharging platform 306, the dilution cup pusher 2002 pushes the dilution cup 2001 that has completed dilution to the disposal port 27, and the reagent card pusher 21 pushes the reagent card 701 that has completed testing to the disposal port 27;

[0191] (16) After the test procedure is completed, the test results can be viewed on the instrument display screen 26 and printed using the built-in printer.

[0192] Emergency testing process:

[0193] (1) Turn on the power switch and wait for the instrument to initialize and be ready;

[0194] (2) After the project is imported, a test tube rack 9 with a sample tube 4 is placed on the sample injection platform 304, and a reagent card 701 is placed in the reagent compartment 703, and a diluent cup 2001 is placed in the diluent compartment 20, and patient information, sample information, and items to be tested are entered;

[0195] (3) Open the emergency sample placement position, insert the emergency test tube into the emergency sample test tube slot, push the emergency sample placement position 10 back, click the emergency button 11, and the emergency sample tube detection sensor 12 detects that the emergency sample has been inserted and starts the detection process. The sample pusher 3011 pushes the test tube rack 9 to the horizontal motion track 305, and then the above normal detection process steps (4)-(14), (16) are followed.

[0196] Alternatively, during the normal testing process, execute step (3) in the above emergency testing process.

[0197] It should be noted that, in this embodiment, the reagent card sample adding position and the sampling position, dilution position, and cleaning position of the sample tube are in a straight line, see Fig.33 The reagent card scanning position is between the reagent compartment and the reagent sample adding position. The reagent card detection position is within the movement range of the detection component. The reagent card detection position is prioritized at the farthest position (right), and is arranged in order as the number of reagent cards increases (arranged to the left).

[0198] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A fully automatic fluorescent immunoassay analyzer, comprising a bracket, the bracket being arranged on a base, characterized in that: A sample injection and sample discharging device is arranged on the base, and the sample injection and sample discharging device is used to push the sample tube into the sample for testing and push the sample tube out for discharging; A sample tube mixing component is arranged on the side of the sample injection and sample output device, and the sample tube mixing component is used to mix the sample in the sample tube, wherein the sample tube is pushed into the sample injection device for testing and pushed out for sample output; It also includes a sampling needle motion assembly, which is used to extract the sample in the sample tube, and the sampling needle is installed on the sampling needle motion assembly; A reagent compartment mechanism is arranged in the bracket, and a plurality of reagent cards are stacked in the reagent compartment mechanism. The sampling needle adds the diluted sample to the sample adding port of the reagent card; The bracket is also provided with an optical path detection component for detecting the reagent card; The sample adding position of the reagent card is in a straight line with the sampling position, dilution position and cleaning position of the sample tube; A dilution cup bin is also provided in the bracket, and a plurality of dilution cups are placed in the dilution cup bin; a dilution cup hand is provided at the bottom of the dilution cup bin, and a dilution cup hand motor drives a dilution cup hand driving wheel and a dilution cup hand driven wheel to drive the dilution cup hand to move the dilution cup to a dilution position; a dilution cup hand driving wheel and a dilution cup hand driven wheel are provided with a dilution cup hand belt, and the dilution cup hand belt is connected to a dilution cup hand slider assembly, and the dilution cup hand is connected to the dilution cup hand slider assembly; correspondingly, a diluent bottle is also provided in the bracket.

2. The fully automatic fluorescent immunoassay analyzer according to claim 1, characterized in that: The sample injection and sample discharge device includes a sample injection push handle structure, a sample injection horizontal push handle structure and a sample discharge push handle structure; wherein the sample injection push handle structure is used to push the sample tube placed on the sample injection platform in the longitudinal direction for sample injection; the sample injection horizontal push handle structure is used to push the sample tube in the transverse direction for sample injection for detection; and the sample discharge push handle structure is used to push the detected sample tube to the sample discharge platform for sample discharge.

3. The fully automatic fluorescent immunoassay analyzer according to claim 2, characterized in that: The sample injection push handle structure comprises a sample injection push handle, which is arranged on a guide rail through a slider assembly; the slider assembly is fixed on a belt, and a motor drives the slider assembly to move back and forth longitudinally along the guide rail through a driving wheel, a driven wheel and the belt, thereby pushing the sample tube longitudinally into the sample injection.

4. The fully automatic fluorescent immunoassay analyzer according to claim 2, characterized in that: The sample discharging pusher structure includes a sample discharging pusher, which is arranged on a guide rail three through a slider assembly three; the slider assembly three is fixed on a belt three, and a motor three drives the slider assembly three to move back and forth longitudinally along the guide rail three through a driving wheel three, a driven wheel three and the belt three, thereby pushing the tested sample tube to the sample discharging platform for sampling.

5. The fully automatic fluorescent immunoassay analyzer according to any one of claims 2 to 4, characterized in that: The sample injection horizontal push handle structure has a plurality of horizontal push handles, which are arranged on the guide rail 2 through the slider assembly 2; the slider assembly 2 is fixed on the belt 2, and the motor 2 drives the slider assembly 2 to move back and forth horizontally along the guide rail 2 through the driving wheel 2, the driven wheel 2 and the belt 2, so as to push the sample tube horizontally into the sample for testing; the sample tube is placed on a test tube rack with a plurality of test tube rack positioning grooves, and the distance between two adjacent horizontal push handles is not greater than the length of the test tube rack.

6. The fully automatic fluorescent immunoassay analyzer according to claim 5, characterized in that: The sample injection and sample output device also includes a transverse motion track, and the test tube rack performs sample injection and sample output along the transverse motion track.

7. The fully automatic fluorescent immunoassay analyzer according to claim 1, characterized in that: The sample tube mixing component has a swing arm for swinging the sample tube, and the swing arm is connected to the rotating shaft of the swing motor to drive the sample tube to swing left and right to mix the sample inside the sample tube.

8. The fully automatic fluorescent immunoassay analyzer according to claim 7, characterized in that: The swing motor is connected to the horizontal slider assembly, and the horizontal slider assembly is driven by the horizontal motor to move horizontally along the horizontal guide rail; the horizontal motor is connected to the upper and lower fixed blocks, and the upper and lower fixed blocks are connected to the upper and lower slider assemblies, and the upper and lower slider assemblies are arranged on the upper and lower guide rails; the upper and lower fixed blocks are driven by the upper and lower motors to move up and down along the upper and lower guide rails.

9. The fully automatic fluorescent immunoassay analyzer according to claim 8, characterized in that: The upper and lower motors and the horizontal motor are both screw motors; the upper and lower motors are provided with upper and lower motion optical couplers on the side, and correspondingly, the upper and lower slider assemblies are provided with upper and lower motion sensing pieces; the horizontal motors are provided with horizontal motion optical couplers on the side, and correspondingly, the horizontal slider assemblies are provided with horizontal motion sensing pieces.

10. The fully automatic fluorescent immunoassay analyzer according to claim 8, characterized in that: The swing arm is provided with a spring clip for clamping the sample tube to mix the sample.

11. The fully automatic fluorescent immunoassay analyzer according to claim 1, characterized in that: The sampling needle movement assembly includes a sampling needle up and down movement assembly and a sampling needle horizontal movement assembly; wherein, the sampling needle up and down movement assembly has a sampling needle up and down movement motor, the sampling needle is arranged on the sampling needle up and down movement slider assembly in the sampling needle movement assembly, and the sampling needle up and down movement motor drives the sampling needle up and down movement slider assembly to reciprocate along the sampling needle up and down guide rails; the sampling needle horizontal movement assembly has a sampling needle horizontal movement motor, the sampling needle up and down movement assembly is connected to the sampling needle horizontal movement slider assembly through a fixed vertical plate, and the sampling needle horizontal movement motor drives the sampling needle horizontal movement slider assembly to reciprocate on the sampling needle horizontal guide rail.

12. The fully automatic fluorescent immunoassay analyzer according to claim 11, characterized in that: The sampling needle up and down movement motor is a screw motor, and the sampling needle up and down movement slider assembly is sleeved on the sampling needle up and down movement motor screw connected to the sampling needle up and down movement motor; the sampling needle horizontal movement motor is a stepping motor, and the sampling needle horizontal movement motor drives the sampling needle horizontal movement driving wheel to rotate, and the sampling needle horizontal movement driven wheel is driven by the sampling needle horizontal movement belt sleeved on the sampling needle horizontal movement driving wheel, and the fixed vertical plate is connected to the sampling needle horizontal movement belt through the sampling needle horizontal movement slider assembly.

13. The fully automatic fluorescent immunoassay analyzer according to claim 12, characterized in that: The sampling needle motion assembly also includes a guide bending belt, one end of which is fixed above the fixed vertical plate, and the other end is arranged on the sampling needle motion assembly support frame, and the guide bending belt is longitudinally arranged along the sampling needle horizontal guide rail.

14. The fully automatic fluorescent immunoassay analyzer according to claim 13, characterized in that: A sampling needle vertical movement limiting optical coupler is arranged on the fixed vertical plate and located at the upper end of the sampling needle upper and lower guide rails; a sampling needle horizontal movement limiting optical coupler is arranged on the sampling needle movement component support frame and located at the end close to the sampling needle horizontal guide rail.

15. The fully automatic fluorescent immunoassay analyzer according to claim 14, characterized in that: The sampling needle is arranged on the sampling needle up and down moving slide block assembly; a dilution needle is also arranged on the fixed vertical plate.

16. The fully automatic fluorescent immunoassay analyzer according to claim 1, characterized in that: The reagent chamber mechanism comprises a reagent chamber support arranged on a base, a plurality of mutually independent reagent chambers are placed on the reagent chamber support, and a plurality of reagent cards can be stacked in each reagent chamber; Correspondingly, a plurality of mutually independent reagent bin shifting devices are arranged at the bottom of each reagent bin and located in the reagent bin bracket; the reagent bin shifting device is used to shift the reagent card in the reagent bin to the reagent card detection track located in the bracket.

17. The fully automatic fluorescent immunoassay analyzer according to claim 16, characterized in that: A reagent card detector is installed on the inner wall of the reagent bin, and the reagent card detector is used to detect the number of reagent cards; a fixing block corresponding to each reagent bin is also arranged on the reagent bin bracket; and a magnet is arranged on the reagent bin or the fixing block.

18. The fully automatic fluorescent immunoassay analyzer according to claim 16, characterized in that: The reagent bin hand-pulling device comprises a reagent bin hand-pulling motor, which drives a hand-pulling slider assembly fixedly connected to the hand-pulling belt to reciprocate along a hand-pulling guide rail through a hand-pulling driving wheel, a hand-pulling driven wheel and a hand-pulling belt; a reagent bin hand-pulling motor is arranged on the hand-pulling slider assembly.

19. The fully automatic fluorescent immunoassay analyzer according to claim 18, characterized in that: The reagent bin dialer includes a dialer head, and an elastic member is arranged below the dialer head; the reagent bin dialer also has a dialer tail movably connected to the dialer slider assembly, and the dialer head is connected to the dialer tail through an inclined portion; an upper pressing plate is arranged on the reagent bin bracket.

20. The fully automatic fluorescent immunoassay analyzer according to claim 19, characterized in that: The reagent bin is also provided with a reagent bin handle; and the fixing block is provided with a reagent bin push-in-place sensor.

21. The fully automatic fluorescent immunoassay analyzer according to any one of claims 16 to 20, characterized in that: The reagent compartment bracket is also provided with a plurality of mutually independent reagent compartment slots; the reagent compartment slots are connected and fixed above by a fixing plate.

22. The fully automatic fluorescent immunoassay analyzer according to claim 1, characterized in that: The optical path detection component is located in the bracket, and includes an optical path detection component fixing block, the optical path detection component fixing block is connected to the optical path detection component slider assembly, and the optical path detection component slider assembly is located on the optical path detection component translation guide rail; the optical path detection component also includes an optical path detection component translation motor, and the optical path detection component translation motor drives the optical path detection component fixing block to reciprocate along the optical path detection component translation guide rail; a light collecting head assembly is installed on the optical path detection component fixing block.

23. The fully automatic fluorescent immunoassay analyzer according to claim 22, characterized in that: A light-collecting head motion motor is also installed on the optical path detection component fixing block. The light-collecting head component is connected to the light-collecting head slider component. The light-collecting head motion motor drives the light-collecting head component to reciprocate along a direction perpendicular to the translation guide rail of the optical path detection component.

24. The fully automatic fluorescent immunoassay analyzer according to claim 23, characterized in that: The light-collecting head assembly comprises an upper shielding cover and a lower light-collecting head; through a window arranged on the optical path detection assembly fixing block, the light-collecting head assembly can move back and forth to collect light signals from the reagent card detection window located below.

25. The fully automatic fluorescent immunoassay analyzer according to claim 24, characterized in that: The optical path detection component fixing block is connected to one end of the detection component guide bending belt, and the detection component guide bending belt is arranged along the direction of the optical path detection component translation guide rail and is located on the side of the optical path detection component translation guide rail.

26. The fully automatic fluorescent immunoassay analyzer according to any one of claims 1-3, 7-20, 22-24, characterized in that: The fully automatic fluorescent immunoassay analyzer also includes an emergency sample placement position, which is used to place a sample tube and is provided with an emergency position and an emergency sample tube detection sensor; the emergency sample placement position is located in the horizontal movement direction of the sampling needle.

27. The fully automatic fluorescent immunoassay analyzer according to claim 26, characterized in that: The fully automatic fluorescent immunoassay analyzer is provided with a test tube rack detection sensor, a test tube rack positioner and a sample tube detection sensor at the rear side of the sample injection and sample output device; wherein the test tube rack detection sensor is installed on the vertical plate of the bracket, and the test tube rack positioner and the sample tube detection sensor are sensed through corresponding windows of the vertical plate of the bracket.

28. The fully automatic fluorescent immunoassay analyzer according to claim 27, characterized in that: A sample code scanner is arranged above the sample tube detection sensor; a code scanning rotating mechanism is arranged near the sample code scanner, and the code scanning rotating mechanism includes a sample tube rotating motor, and the sample tube rotating motor drives the rotating wheel to rotate; the code scanning rotating mechanism also includes a rotating wheel 1 and a rotating wheel 2, and the rotating wheel 1 and the rotating wheel 2 are both connected with a tensioning spring, and there is a gap between the rotating wheel 1 and the rotating wheel 2 and the rotating wheel.

29. The fully automatic fluorescent immunoassay analyzer according to any one of claims 1-3, 7-20, 22-24, characterized in that: A reagent card scanner is arranged in the bracket, and the reagent card scanner is located above the reagent card detection track; a sampling needle cleaning component is also arranged in the bracket.

30. The fully automatic fluorescent immunoassay analyzer according to any one of claims 1-3, 7-20, 22-24, characterized in that: A reagent card pusher for pushing the reagent card is arranged on the reagent card detection track. The reagent card pusher drives a reagent card pusher driving wheel and a reagent card pusher driven wheel through a reagent card pusher motor, thereby driving the reagent card pusher to push the reagent card to the incubation detection position.

31. The fully automatic fluorescent immunoassay analyzer according to any one of claims 1-3, 7-20, 22-24, characterized in that: The outside of the bracket is also covered with a shell, and a display screen is arranged on the shell; a disposal port and an instrument gripper are also arranged on the side of the shell; the fully automatic fluorescent immunoassay analyzer is also provided with a built-in printer, and the paper outlet of the built-in printer is arranged on the shell; the shell is also provided with an observation window and an item scanner.

32. A sample detection method, using the fully automatic fluorescent immunoassay analyzer of claim 1, characterized in that: The normal detection process includes the following steps: (1) Turn on the power switch and wait for the instrument to initialize and be ready; (2) After the project is imported, place the test tube rack with the sample tube, put in the reagent card, put in the diluent cup, and enter the patient information, sample information and items to be tested; (3) Click the detection button to push the test tube rack to the horizontal motion track. After the test tube rack is detected to be in place, push the test tube rack to the sample tube scanning position. After detecting that there is a sample tube in this hole of the test tube rack, the sample scanner is turned on and the sample tube is slowly rotated so that the barcode on the sample tube faces the sample scanner. The sample scanner scans the barcode and uploads the data; (4) After the sample information is verified to be correct, remove the reagent card from the reagent compartment of the corresponding test item, move the reagent card, scan the reagent card QR code at the reagent card scanning position, and upload the data; (5) After verifying that the reagent card information is correct, push the reagent card to the sample loading position; (6) Move the dilution cup to the dilution position corresponding to the sample addition position; (7) Move the test tube rack to the sample tube mixing position, fork the sample tube, lift the forked sample tube to a certain height, and swing the sample tube back and forth within a certain angle range to mix the sample in the test tube; (8) After mixing is completed, insert the sample tube back into the test tube rack and move the test tube rack to the sampling position; (9) Move the sampling needle to the top of the sample tube, lower the sampling needle so that the sampling needle penetrates into the sample tube, and extract the sample in the sample tube through the sampling needle; (10) After the sample is collected, lift the sampling needle and move it to the top of the dilution cup. Then, inject the sample drawn from the sampling needle into the dilution cup and inject a certain proportion of diluent from the dilution needle into the dilution cup. (11) After the dilution is completed, move the sampling needle to the top of the dilution cup, lower the end of the sampling needle to the bottom of the dilution cup, and draw the diluted sample into the sampling needle; (12) Lift the sampling needle, move the sampling needle to the sample loading position, lower the sampling needle to above the sample loading port of the reagent card on the sample loading position, and inject the sample in the sampling needle into the sample loading port of the reagent card; (13) Push the reagent card with sample loading to the incubation detection position, start the heating module, and keep the reagent card incubated at the set temperature; (14) After the incubation is completed, the detection component is moved to the top of the reagent card, the data acquisition module is started, the light collecting head is moved in the detection window of the reagent card, and the data acquisition module obtains the scanning signal; (15) Move the test tube rack to the sample dispensing position, push the test tube rack onto the sample dispensing platform, push the completed dilution cup to the disposal port, and push the completed reagent card to the disposal port; (16) After the test procedure is completed, the test results can be viewed on the instrument display and printed using the built-in printer.

33. The sample detection method according to claim 32, characterized in that: Also included is an emergency testing process, which includes the following steps: (1) Turn on the power switch and wait for the instrument to initialize and be ready; (2) After the project is imported, place the test tube rack with the sample tube, put in the reagent card, put in the diluent cup, and enter the patient information, sample information and items to be tested; (3) Open the emergency sample placement position, insert the emergency test tube into the emergency sample test tube slot, push the emergency sample placement position back, click the emergency button, and start the test process after detecting that the emergency sample has been inserted; then follow the normal test process steps (4)-(14), (16); Alternatively, during the normal testing process, execute step (3) in the above emergency testing process.

Images