A microbial sample storage and transfer system

Abstract

The present invention relates to the technical field of microbial sample preservation, and specifically discloses a microbial sample preservation and transfer system. The system includes a sample preservation tube and a sample dispensing tube. The sample preservation tube includes a preservation tube bottle body and a preservation tube bottle cap, and a pressing pump is provided on the preservation tube bottle cap, and the pressing pump includes a guide tube, and one end of the guide tube is placed in the preservation tube bottle body. The sample dispensing tube includes a dispensing tube bottle body, a self-priming part is provided at the bottom, a conducting tube is connected to the self-priming part, and a spring pump is sleeved on the conducting tube, and the spring pump presses down the self-priming part so that the self-priming part closes the conducting tube. One end of the guiding tube placed outside the sample preservation tube can be connected to the spring pump and communicated with the conducting tube; the pressing pump is pressed down by the sample dispensing tube, so that the liquid in the preservation tube bottle body enters the dispensing tube bottle body through the guide tube. This system ensures that the sample solution is transferred from the sample preservation tube to the sample dispensing tube under a sealed condition, which not only ensures the safety of the operator, but also improves the work efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial sample preservation, and in particular to a microbial sample preservation and transfer system. Background Art

[0002] The presence of pathogenic and infectious microorganisms in the human living environment and human body has always been a risk factor for human health. The most effective prevention and control measures for infectious diseases are to promptly detect infected people, track their close contacts, effectively isolate related personnel and treat them. Based on this, the most critical preventive measure is to effectively detect all possible infected people and any possible contacts, thereby confirming and isolating all infected people, so the detection of pathogenic microorganisms is crucial.

[0003] Efficient detection and pathogen protection during the detection process are the key points to complete the detection. At present, the whole process from sampling to detection includes personnel sampling, samples are stored in sample tubes, sample tubes are transported to the laboratory, and the test personnel take out the required amount of liquid from the sample tube to the test tube, and the test tube is placed in a nucleic acid extractor to extract microbial nucleic acid, and the nucleic acid is taken out for detection. In this process, there is a risk of infection to the test personnel, and the process of opening and closing the cover is time-consuming, especially in the process of large-sample detection of the population. Because the single-layer threaded cover sample storage tube is mainly used on the market today, the shape is relatively simple. After the sampling is completed, the sampling test is placed in the sample tube, covered with a threaded cover, and sent to the laboratory. Each time the test is performed, the test personnel need to open and close the cover of the test tube to take out the required amount of liquid that may contain microorganisms, add it to the test tube, and send it to the nucleic acid extractor to extract nucleic acid. Frequent opening and closing of the cover may not only cause the risk of personnel infection, but also lead to the generation of aerosols in the laboratory, causing pollution in the detection area. Because of the possibility that opening the bottle cap may cause microorganisms to infect the laboratory and test personnel, there are currently high requirements for the protection of testing laboratories and test personnel for highly pathogenic and infectious microorganisms.

[0004] At the same time, when a major public health incident occurs, the laboratory has to perform a huge amount of testing every day, which will increase the risk of infection and the psychological pressure of testers. At the same time, the operation of removing the test liquid from each preservation tube with a pipette is also time-consuming and laborious.

[0005] Therefore, there is an urgent need to provide a microbial sample preservation and transfer system to ensure that the sample remains sealed from sampling to testing, so as to reduce the risk of infection for testing personnel and avoid laboratory contamination, while improving the efficiency of liquid collection. It may also be possible to appropriately lower the protection level of the laboratory and testing personnel to facilitate more hospital laboratories to carry out testing. Summary of the invention

[0006] The object of the present invention is to provide a microorganism sample storage and transfer system to realize a simple and airtight transfer operation of the microorganism sample without opening the cover.

[0007] To achieve this object, the present invention adopts the following technical solutions:

[0008] A microbial sample preservation and transfer system includes a sample preservation tube and a sample dispensing tube. The sample preservation tube includes a preservation tube body and a preservation tube cap detachably connected to the preservation tube body, the preservation tube cap is provided with a pressing pump, the pressing pump includes a guide tube, and one end of the guide tube is placed in the preservation tube body. The sample dispensing tube includes a dispensing tube body, a self-priming part is provided at the bottom of the dispensing tube body, a conducting tube is connected to the self-priming part, a spring pump is sleeved on the conducting tube, and the spring pump presses down the self-priming part so that the self-priming part closes the conducting tube. One end of the guiding tube placed outside the sample preservation tube can be connected to the spring pump and communicated with the conducting tube; the pressing pump is pressed down by the sample dispensing tube so that the liquid in the preservation tube body enters the dispensing tube body through the guide tube.

[0009] Among them, a partition plate is arranged in the storage tube bottle body, and the partition plate divides the storage tube bottle body into a accommodating chamber and a liquid collection chamber. A guide tube hole and a plurality of flow holes are opened on the partition plate. The guide tube is placed in the storage tube bottle body at one end, and the flow hole is penetrated and arranged in the liquid collection chamber.

[0010] Preferably, the upper cover of the pressing pump disposed on the outside of the bottle body of the preservation tube is provided with a sealing cover, the sealing cover is card-connected with the pressing pump, and one end of the guide tube disposed on the outside of the bottle body of the preservation tube is also arranged in the sealing cover.

[0011] Furthermore, the storage tube body is threadedly connected to the storage tube cap, a gasket is sleeved on the end of the storage tube body, and the outer wall of the gasket is in contact with the inner wall of the storage tube cap.

[0012] Preferably, the sample dispensing tube further comprises a dispensing tube bottle cap, and the dispensing tube bottle cap is threadedly connected to an end of the dispensing tube bottle body opposite to the self-priming component.

[0013] Preferably, a bottle body clamp is convexly provided on the inner wall of the dispensing tube bottle body along the circumferential direction, an isolation membrane is placed on the bottle body clamp, a sealing ring is provided on the isolation membrane, and the outer side wall of the sealing ring is in contact with the inner wall of the dispensing tube bottle body.

[0014] Preferably, grinding beads are arranged in the accommodating cavity.

[0015] Furthermore, the grinding beads are glass elution beads.

[0016] Preferably, the shape of the bottom end of the storage tube bottle body is a pointed shape protruding outward from the bottle body, and the bottom end of the storage tube bottle body is connected to an anti-falling tube bottle with a flat bottom, and the bottom end of the storage tube bottle body is placed in the anti-falling tube bottle.

[0017] Preferably, the microbial sample storage and transfer system further comprises a tube rack, on which a silicone plug is provided, and the silicone plug cooperates with an arc groove provided at the bottom of the liquid separation tube bottle body.

[0018] Beneficial effects of the present invention:

[0019] Through the cooperation of the flow guide tube and the connecting tube, the liquid sample in the sample storage tube can be transferred to the sample dispensing tube in a closed manner without opening the cover, which can effectively prevent the contamination of the laboratory environment and the infection of personnel caused by the leakage of microorganisms or the generation of aerosols. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is a cross-sectional view of a sample storage tube provided by an embodiment of the present invention;

[0021] Figure 2 is a cross-sectional view of a sample dispensing tube provided in an embodiment of the present invention;

[0022] Figure 3 is a schematic structural diagram of a partition plate provided by an embodiment of the present invention;

[0023] Figure 4 It is a schematic diagram of the structure of a pipe rack provided in an embodiment of the present invention.

[0024] In the figure:

[0025] 1. Sample storage tube; 11. Storage tube bottle body; 111. Partition plate; 1111. Guide tube hole; 1112. Flow hole; 112. Accommodation cavity; 113. Liquid collection cavity; 114. Grinding beads; 115. Anti-fall tube bottle; 12. Storage tube bottle cap; 121. Pressing pump; 122. Guide tube; 123. Sealing cover; 124. Gasket;

[0026] 2. Sample dispensing tube; 21. Dispensing tube bottle body; 211. Self-priming member; 212. Spring pump; 213. Isolation membrane; 214. Bottle body clamp; 215. Sealing ring; 216. Arc groove; 217. Conducting tube; 22. Dispensing tube bottle cap;

[0027] 3. Pipe rack; 31. Silicone plug. DETAILED DESCRIPTION

[0028] In order to make the technical problems solved by the present invention, the technical solutions adopted and the technical effects achieved clearer, the technical solutions of the embodiments of the present invention will be further described in detail below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0029] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0030] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0031] The technical solution of the present invention is further described below with reference to the accompanying drawings and through specific implementation methods.

[0032] like Figure 1 and Figure 2As shown, this embodiment provides a microorganism sample preservation and transfer system, including a sample preservation tube 1 and a sample dispensing tube 2. The sample preservation tube 1 includes a preservation tube body 11 and a preservation tube bottle cap 12 detachably connected to the preservation tube bottle body 11, and a pressing pump 121 is provided on the preservation tube bottle cap 12, and the pressing pump 121 includes a guide tube 122, and one end of the guide tube 122 is placed in the preservation tube bottle body 11. The sample dispensing tube 2 includes a dispensing tube body 21, and a self-priming member 211 is provided at the bottom of the dispensing tube bottle body 21, and a conducting tube 217 is connected to the self-priming member 211, and a spring pump 212 is sleeved on the conducting tube 217, and the spring pump 212 presses down the self-priming member 211 so that the self-priming member 211 closes the conducting tube 217. One end of the flow guide tube 122 placed outside the sample storage tube 1 can be connected to the spring pump 212 and communicated with the conducting tube 217; the sample dispensing tube 2 presses down the pump 121 to allow the liquid in the storage tube bottle 11 to enter the dispensing tube bottle 21 through the flow guide tube 122. Through the cooperation of the flow guide tube 122 and the conducting tube 217, the liquid sample in the sample storage tube 1 can be transferred to the sample dispensing tube 2 in a sealed manner without opening the cover, which can effectively prevent the leakage of microorganisms or the generation of aerosols from causing contamination of the laboratory environment and infection of personnel.

[0033] The storage tube bottle body 11 is provided with a partition plate 111, which divides the storage tube bottle body 11 into a receiving chamber 112 and a liquid collection chamber 113. Figure 1 and Figure 3 The partition plate 111 is provided with a flow guide hole 1111 and a plurality of flow holes 1112. The flow guide tube 122 is placed in the storage tube bottle body 11 and has a flow hole 1112 at one end thereof, which is arranged in the liquid collection cavity 113. A sampling swab is placed in the accommodating cavity 112, and the flow holes 1112 on the partition plate 111 allow the liquid in the accommodating cavity 112 and the liquid collection cavity 113 to flow freely. The partition plate 111 separates the sampling swab in the accommodating cavity 112 from the end of the flow guide tube 122, thereby avoiding the situation where the sample cannot be drawn due to the sampling swab blocking the flow guide tube 122.

[0034] In this embodiment, grinding beads 114 are arranged in the accommodating chamber 112. The arrangement of the grinding beads 114 can effectively improve the release rate of microorganisms. Further, the grinding beads 114 are preferably glass elution beads. Glass elution beads have good effects in promoting the release of microorganisms (such as bacteria and viruses) and are low in cost.

[0035] In this embodiment, a sealing cover 123 is provided on the upper cover of the pressing pump 121 disposed outside the storage tube body 11, and the sealing cover 123 is connected to the pressing pump 121 in a card-type connection, and one end of the guide tube 122 disposed outside the storage tube body 11 is also disposed in the sealing cover 123. The provision of the sealing cover 123 ensures that the sample in the sample storage tube 1 will not enter the external environment; the card-type connection simplifies the operation steps of the operator and improves the efficiency of the sampling operation.

[0036] The preservation tube body 11 is threadedly connected to the preservation tube cap 12, and a gasket 124 is sleeved on the end of the preservation tube body 11, and the outer wall of the gasket 124 fits the inner wall of the preservation tube cap 12. The provision of the gasket 124 further ensures the sealed connection between the preservation tube body 11 and the preservation tube cap 12.

[0037] In this embodiment, the shape of the bottom end of the preservation tube bottle body 11 is a sharp angle protruding out of the bottle body, and the bottom end of the preservation tube bottle body 11 is connected to an anti-falling tube bottle 115 with a flat bottom, and the bottom end of the preservation tube bottle body 11 is placed in the anti-falling tube bottle 115. The design of the sharp-angled bottle bottom is suitable for centrifugation and shaking operations, and the anti-falling tube bottle 115 is provided to facilitate the placement of the sample preservation tube 1.

[0038] Continue to refer to Figure 2 The sample dispensing tube 2 also includes a dispensing tube bottle cap 22, which is threadedly connected to the end of the dispensing tube bottle body 21 opposite to the self-priming member 211. The sealing structure design of the sample preservation tube 1 and the sample dispensing tube 2 avoids cross contamination, can meet the transportation under low temperature, high temperature, aviation and negative pressure conditions at the same time, and meets the requirements for the transportation and storage of infectious pathogens and microorganisms.

[0039] The inner wall of the dispensing tube bottle body 21 is provided with a bottle body clamp 214 protruding along the circumferential direction, and an isolation film 213 is placed on the bottle body clamp 214. A sealing ring 215 is provided on the isolation film 213, and the outer wall of the sealing ring 215 is in contact with the inner wall of the dispensing tube bottle body 21. The dispensing film 213 is used to limit the capacity of the dispensing tube bottle body 21. In subsequent operations, the isolation film 213 can be punctured.

[0040] like Figure 4 As shown, the microbial sample storage and transfer system also includes a tube rack 3, on which a silicone plug 31 is provided, and the silicone plug 31 cooperates with the arc groove 216 provided at the bottom of the liquid dispensing tube bottle 21. The tube rack 3 provides a good placement environment for the sample liquid dispensing tube 2. While facilitating the transportation of the sample liquid dispensing tube 2, it also avoids the situation where the sample in the liquid dispensing tube bottle 21 flows out due to accidental touching of the self-priming part 211. In this embodiment, the self-priming part 211 is arranged in the center of the arc groove 216. The arc groove 216 cooperates with the silicone plug 31 to seal the self-priming part 211 to prevent the self-priming part 211 from being opened due to external force.

[0041] In this embodiment, the capacity of the preservation tube bottle 11 is 5-10 mL. The capacity of the liquid dispensing tube bottle 21 is 200-400 μL. The material of the preservation tube bottle 11 is PP, PE or PVC. During the sampling operation, the preservation tube bottle 11 contains 2-4 mL of microorganism preservation / lysis solution.

[0042] In the present embodiment, the pressing pump 121 also includes a pump body and a spring installed in the pump body. When the guide tube 122 moves downward relative to the pump body, the upper end of the guide tube 122 provides suction to the lower end. When not affected by external force, the self-priming member 211 is connected. Under the influence of external force, the self-priming member 211 shrinks violently inward, causing the self-priming member 211 to be closed. The spring pump 212 includes an upper pump body and a lower pump body that are movably connected, and a spring installed in the splicing space between the upper pump body and the lower pump body. The spring pump 212 expands and contracts as the external force applied to it changes. The above structures are all prior art and will not be described in detail here.

[0043] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the embodiments here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A microbial sample storage and transfer system, characterized in that: include: A sample preservation tube (1) comprises a preservation tube body (11) and a preservation tube cap (12) detachably connected to the preservation tube body (11), wherein the preservation tube cap (12) is provided with a pressing pump (121), the pressing pump (121) comprises a flow guide tube (122), and one end of the flow guide tube (122) is placed in the preservation tube body (11); A sample dispensing tube (2), comprising a dispensing tube bottle body (21), wherein a self-priming component (211) is arranged at the bottom of the dispensing tube bottle body (21), wherein a conducting tube (217) is connected to the self-priming component (211), wherein a spring pump (212) is sleeved on the conducting tube (217), wherein the spring pump (212) presses down the self-priming component (211) so that the self-priming component (211) closes the conducting tube (217); One end of the guide tube (122) disposed outside the sample storage tube (1) can be connected to the spring pump (212) and communicated with the guide tube (217); the pressing pump (121) is pressed down through the sample dispensing tube (2) so that the liquid in the storage tube bottle (11) enters the dispensing tube bottle (21) through the guide tube (122); The microbial sample storage and transfer system further comprises a tube rack (3), the tube rack (3) being provided with a silicone plug (31), the silicone plug (31) being matched with an arc-shaped groove (216) provided at the bottom of the liquid dispensing tube bottle body (21); the self-priming member (211) being provided at the center of the arc-shaped groove (216).

2. The microbial sample storage and transfer system according to claim 1, characterized in that: A partition plate (111) is arranged inside the preservation tube body (11), and the partition plate (111) divides the preservation tube body (111) into a containing chamber (112) and a liquid collection chamber (113). A flow guide hole (1111) and a plurality of flow holes (1112) are provided on the partition plate (111), and one end of the flow guide tube (122) disposed inside the preservation tube body (11) passes through the flow hole (1112) and is disposed in the liquid collection chamber (113).

3. The microbial sample storage and transfer system according to claim 1, characterized in that: The upper cover of the pressing pump (121) disposed outside the storage tube body (11) is provided with a sealing cover (123), the sealing cover (123) is card-connected to the pressing pump (121), and one end of the guide tube (122) disposed outside the storage tube body (11) is also disposed inside the sealing cover (123).

4. The microbial sample storage and transfer system according to claim 3, characterized in that: The preservation tube body (11) is threadedly connected to the preservation tube cap (12); a gasket (124) is sleeved on the end of the preservation tube body (11), and the outer wall of the gasket (124) is in contact with the inner wall of the preservation tube cap (12).

5. The microbial sample storage and transfer system according to claim 1, characterized in that: The sample dispensing tube (2) further comprises a dispensing tube bottle cap (22), wherein the dispensing tube bottle cap (22) is threadedly connected to an end of the dispensing tube bottle body (21) opposite to the self-priming member (211).

6. The microbial sample storage and transfer system according to claim 1, characterized in that: The inner wall of the dispensing tube bottle body (21) is provided with a bottle body clamp (214) protruding along the circumferential direction, an isolation film (213) is placed on the bottle body clamp (214), a sealing ring (215) is provided on the isolation film (213), and the outer wall of the sealing ring (215) is in contact with the inner wall of the dispensing tube bottle body (21).

7. The microbial sample storage and transfer system according to claim 2, characterized in that: Grinding beads (114) are arranged in the accommodating cavity (112).

8. The microbial sample storage and transfer system according to claim 7, characterized in that: The grinding beads (114) are glass elution beads.

9. The microbial sample storage and transfer system according to claim 1, characterized in that: The bottom end of the storage tube bottle body (11) is in the shape of a pointed angle protruding outward from the bottle body, and the bottom end of the storage tube bottle body (11) is connected to an anti-falling tube bottle (115) having a flat bottom, and the bottom end of the storage tube bottle body (11) is placed in the anti-falling tube bottle (115).

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