Perforated plate filtration device and method

Abstract

The present invention provides a porous plate filtering device and method, including a porous plate, a clamp, a moving assembly, a guide block, an air positive pressure block and a frame; the moving assembly, the guide block and the air positive pressure block are all arranged on the frame, the clamp is connected to the moving assembly and can move under the action of the moving assembly, the clamp can grab the porous plate, the porous plate is installed on the guide block, and the guide block is located below the air positive pressure block; the porous plate includes a grid, a filter membrane and a filtrate plate, the filter membrane is connected to the bottom of the grid, the filtrate plate is detachably connected below the filter membrane, and the edges on both sides of the porous plate are turned outward to form a clamping position. The present invention has a simple and reasonable structure, and can filter multiple samples at the same time by adopting a porous plate, which is suitable for batch sample preparation. The present invention realizes filtration by cooperating with the air positive pressure block and the filter membrane, replacing traditional centrifugal filtration, saving time and effort.

Description

Technical Field

[0001] The present invention relates to the technical field of medical instruments, and in particular to a porous plate filtering device and method. Background Art

[0002] When analyzing cells, cell nuclei or other organelles, the sample needs to be filtered first to remove large particle impurities therein, thereby filtering to obtain a filtrate containing target cells or cell nuclei or organelles, and the obtained filtrate usually needs to be centrifuged to collect the target cells or cell nuclei or organelles. At present, the sample filtration chamber usually filters the sample into a container first, and then transfers the filtrate in the container into a centrifuge tube. The process is complicated and prone to bacterial contamination. Although a patent has reported a filter that directly filters the filtrate into a centrifuge tube, the filtration speed is slow.

[0003] The patent document with the publication number CN209226978U discloses a placental tissue stem cell filtration device, including a stem cell filter, a tissue fluid receiving chamber, a tissue fluid recovery chamber and a mounting bracket, wherein the mounting bracket is provided with a base and a height adjustment mechanism, wherein the tissue fluid receiving chamber is arranged on the upper part of the mounting bracket through the height adjustment mechanism, and the lower part of the tissue fluid receiving chamber is a stem cell filter, which is connected to the tissue fluid recovery chamber through a drainage tube, and the tissue fluid recovery chamber is arranged on the base; the stem cell filter includes an upper opening of the stem cell filter, a filter membrane, a filter membrane carrier platform and a lower opening of the stem cell filter, and the filter membrane is placed on the filter membrane carrier platform; a rubber cap is installed on the lower opening of the stem cell filter, and the upper opening of the stem cell filter is connected to the tissue fluid receiving chamber, and the lower opening of the stem cell filter is connected to the tissue fluid recovery chamber through a drainage tube. The technical solution in the patent document can only realize the preparation of one sample at a time, and is not applicable to batch sample preparation. Summary of the invention

[0004] In view of the defects in the prior art, an object of the present invention is to provide a porous plate filtering device and method.

[0005] According to one aspect of the present invention, there is provided a porous plate filtering device, comprising a porous plate, a clamping claw, a moving assembly, a guide block, an air blowing positive pressure block and a frame;

[0006] The moving assembly, guide block and air positive pressure block are all arranged on the frame. The clamp is connected to the moving assembly and can move under the action of the moving assembly. The clamp can grab the porous plate. The porous plate is installed on the guide block, and the guide block is located below the air positive pressure block.

[0007] Preferably, the moving assembly comprises an X-axis slide rail and a Y-axis slide rail, the Y-axis slide rail is mounted on the X-axis slide rail and can move on the X-axis slide rail, and the clamp is mounted on the Y-axis slide rail and can move on the Y-axis slide rail.

[0008] Preferably, the guide block is movably connected to the frame, and the guide block can translate and rotate below the air blowing positive pressure block.

[0009] Preferably, the air blowing positive pressure block is movably connected to the frame, and the air blowing positive pressure block can move horizontally and / or rotate above the guide block;

[0010] The air blowing positive pressure block can be connected to external gas through a pipeline.

[0011] Preferably, the porous plate comprises a grid, a filter membrane and a filtrate tray, the filter membrane is connected to the bottom of the grid, the filtrate tray is detachably connected below the filter membrane, and the edges on both sides of the porous plate are turned outward to form a clamping position.

[0012] Preferably, one or more clamping strips are provided at the bottom of the edge of the porous plate, and one or more clamping slots are provided on the clamping jaws. When the clamping jaws are clamped at the clamping position of the porous plate, the clamping strips are matched and connected with the clamping slots in a one-to-one correspondence;

[0013] A cut corner is arranged on one side edge of the porous plate to form a placement mark.

[0014] Preferably, there are a plurality of holes, and the plurality of holes are arranged in a plurality of rows and columns; and the holes are in a gate arch shape, a T shape or a triangle shape.

[0015] Preferably, the filter membrane has a filtration pore diameter of 5-50 microns; and the filter membrane is welded to the edges of the bottom of the pores.

[0016] Preferably, it further comprises a stacking assembly, wherein the stacking assembly comprises multiple layers of placement positions, and the placement positions are capable of placing multi-well plates.

[0017] According to another aspect of the present invention, a porous plate filtration method is provided, using the porous plate filtration device, comprising the following steps:

[0018] Step 1: placing the porous plate into the stack assembly;

[0019] Step 2: The clamping claw moves to the stacking assembly through the moving assembly and clamps the porous plate at the clamping position, and then moves to the guide block through the moving assembly and installs the porous plate on the guide block;

[0020] Step 3: The guide block moves so that one side of the porous plate tilts upward;

[0021] Step 4: Add the sample to the wells of the multi-well plate through an external sample adding device;

[0022] Step 5: Start the positive air pressure block to blow air and apply pressure to the porous plate for filtering, and filter the holes on the porous plate in a single row one by one by moving the positive air pressure block or the guide block.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. The present invention has a simple and reasonable structure and can filter multiple samples at the same time by using a porous plate, which is suitable for batch sample preparation.

[0025] 2. The present invention realizes filtration by cooperating the air-blowing positive pressure block with the filter membrane, replacing the traditional centrifugal filtration, saving time and labor.

[0026] 3. The bottom of the porous plate of the present invention can be detachably connected to the filtrate tray, which is convenient for collecting and pouring the filtrate.

[0027] 4. The present invention provides a clamping strip at the bottom of the edge of the porous plate and a clamping groove on the clamping jaws. When the clamping jaws clamp the porous plate, the clamping strip and the clamping groove are matched and connected to prevent the porous plate from sliding relative to the clamping jaws during the grabbing and moving process.

[0028] 5. A cut corner is provided on one edge of the porous plate of the present invention to form a placement mark, so as to prevent the porous plate from being placed upside down in the stacking assembly, thereby affecting subsequent operations. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Other features, objects and advantages of the present invention will become more apparent from the detailed description of non-limiting embodiments made with reference to the following drawings:

[0030] Figure 1 It is a structural schematic diagram of the present invention.

[0031] Figure 2 It is a schematic diagram of the three-dimensional structure of the porous plate of the present invention.

[0032] Figure 3 It is a schematic diagram of the bottom view of the structure of the porous plate of the present invention.

[0033] Figure 4 It is a schematic diagram of the clamping jaws of the present invention clamping a porous plate.

[0034] The figure shows:

[0035] Perforated plate 1301 Y-axis slide rail 1306

[0036] Gripper 1302 Frame 1307

[0037] Guide block 1303 Stack assembly 1308

[0038] Air positive pressure block 1304 Card strip 1309

[0039] X-axis slide rail 1305 Placement mark 1310 DETAILED DESCRIPTION

[0040] The present invention is described in detail below in conjunction with specific embodiments. The following embodiments will help those skilled in the art to further understand the present invention, but are not intended to limit the present invention in any form. It should be noted that, for those of ordinary skill in the art, several changes and improvements can also be made without departing from the concept of the present invention. These all belong to the protection scope of the present invention.

[0041] In the description of the present application, it should be understood that the terms "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0042] According to one aspect of the present invention, a porous plate filtration device is provided. Figure 1 As shown, it includes a porous plate, a clamp, a moving assembly, a guide block, an air positive pressure block and a frame; the moving assembly, the guide block and the air positive pressure block are all arranged on the frame, the clamp is connected to the moving assembly and can move under the action of the moving assembly, the clamp can grab the porous plate, the porous plate is installed on the guide block, and the guide block is located below the air positive pressure block. The moving assembly includes an X-axis slide rail and a Y-axis slide rail, the Y-axis slide rail is installed on the X-axis slide rail and can move on the X-axis slide rail, the clamp is installed on the Y-axis slide rail and can move on the Y-axis slide rail. It also includes a stacking assembly, the stacking assembly includes multiple layers of placement positions, and the placement positions can place the porous plate.

[0043] The guide block is movably connected to the frame, and the guide block can move horizontally and rotate below the air positive pressure block. The air positive pressure block is movably connected to the frame, and the air positive pressure block can move horizontally and / or rotate above the guide block; the air positive pressure block can be connected to external gas through a pipeline.

[0044] like Figure 2-4As shown, the porous plate includes a grid, a filter membrane and a filtrate plate. The filter membrane is connected to the bottom of the grid, and the filtrate plate is detachably connected below the filter membrane. The edges of the porous plate are turned outward to form a clamping position. One or more clips are provided at the bottom of the edge of the porous plate, and one or more slots are provided on the clamping jaws. When the clamping jaws are clamped in the clamping position of the porous plate, the clips and the slots are matched and connected one by one; a cut corner is provided on one edge of the porous plate to form a placement mark. There are multiple grids, and the multiple grids are arranged in multiple rows and columns; the grids are arched, T-shaped or triangular. The diameter of the filter hole of the filter membrane is 5-50 microns; the filter membrane is welded on the edge at the bottom of the grid, that is, the welding point is located on the edge at the bottom of the grid. When the filtrate or air pressure in the grid presses the filter membrane downward, the filter membrane can be stretched to prevent wrinkles from appearing on the filter membrane, thereby achieving a better filtering effect.

[0045] Preferably, the porous plate includes 24 gate arch-shaped holes, arranged in 6 rows and 4 columns, the filter membrane adopts a screen, the screen is welded to the bottom of the hole by a heat welding process, the diameter of the arc portion of the gate arch hole is φ4-50 mm, the width of the straight edge of the gate arch hole is 4-50 mm, the depth of the gate arch hole is 5-40 mm, the liquid filling amount of the porous plate is 20-3000 μL, and the porous plate is made of PES (polyethersulfone resin), PE (polyethylene) or PET (polyethylene terephthalate).

[0046] According to another aspect of the present invention, a porous plate filtration method is provided, using the porous plate filtration device, comprising the following steps:

[0047] Step 1: placing the porous plate into the stack assembly;

[0048] Step 2: The clamp moves to the stacking assembly through the moving assembly and clamps the porous plate at the clamping position, and then moves to the guide block through the moving assembly, and installs the porous plate on the guide block; preferably, the clamp moves into the stacking assembly through the X-axis slide rail to grab the porous plate, and grabs each layer of the porous plate in the stacking assembly through the movement of the Y-axis slide rail, and sequentially grabs a layer of porous plates from top to bottom and places the porous plate on the guide block through the movement of the X-axis slide rail;

[0049] Step 3: the guide block moves so that one side of the porous plate tilts upward; preferably, when the grid is a gate arch, the placement mark is set on the opposite side of the gate arch arc, and the placement mark is located on the inner side of the stack assembly when placed, the clamp takes the orifice plate out of the stack assembly and places it on the guide block, and the guide block moves so that the end of the porous plate with the placement mark tilts upward, so that the arc-shaped end of the gate arch is located at the lowest point, and the straight end of the gate arch is located at the highest point, that is, the lowest point of the grid has a convergence effect, so that the filtered material gathers at the lowest point after filtration; when the grid is T-shaped, the porous plate is tilted so that the bottom end of the T-shaped vertical arm is located at the lowest point, and the horizontal arm of the T-shape is located at the highest point; when the grid is a triangle, the orifice plate is tilted so that the vertex of the triangle is located at the lowest point, and the side of the triangle is located at the highest point; preferably, the angle at which the porous plate tilts upward is 5-30°;

[0050] Step 4: dripping the sample into the wells of the multi-well plate through an external sample loading device; preferably, the samples are loaded one by one in a single row, and the samples are loaded at the highest point of each inclined well, and the sample loading device uses a TIP head for loading;

[0051] Step 5: Start the positive air pressure block to blow air to the porous plate for filtering, and filter the holes on the porous plate in a single row by moving the positive air pressure block or the guide block. The filtered sample is taken out at the lowest point of the inclined hole grid through the sampling device and enters the next process. The sampling device preferably uses a TIP head for sampling.

[0052] In one embodiment, the present invention is applied to separate cells from a cell preservation solution or a cell stock solution, filter the liquid through a filter membrane, retain the desired cells, filter cell impurities, and preserve the cell morphology well. The present invention can be applied to TCT inspection (liquid-based thin layer cell detection). The clamp moves from top to bottom in order to grab the eight porous plates placed in the stack assembly through the Y-axis slide; the clamp moves the grabbed porous plate through the X-axis slide to place the porous plate in the guide block for fixing; after fixing the porous plate, the TIP head draws the cell stock solution from the sample cup and places it in the pores in the porous plate; the air-blowing positive pressure block moves down to the porous plate position through the guide rail, and the air-blowing positive pressure block performs air-blowing pressure filtration, and pushes the porous plate forward one grid after each row of filtration, until all the 4 rows × 6 rows of pores of the sieve plate are used up, and the next porous plate is replaced.

[0053] The above describes the specific embodiments of the present invention. It should be understood that the present invention is not limited to the above specific embodiments, and those skilled in the art can make various changes or modifications within the scope of the claims, which does not affect the essence of the present invention. In the absence of conflict, the embodiments of the present application and the features in the embodiments can be combined with each other arbitrarily.

Claims

1. A porous plate filtration device, characterized in that: It includes a porous plate, a clamping claw, a moving assembly, a guide block, an air blowing positive pressure block and a frame; The moving assembly, the guide block, and the air blowing positive pressure block are all arranged on the frame, the clamp is connected to the moving assembly and can move under the action of the moving assembly, the clamp can grab the porous plate, the porous plate is installed on the guide block, and the guide block is located below the air blowing positive pressure block; The moving assembly comprises an X-axis slide rail and a Y-axis slide rail, the Y-axis slide rail is mounted on the X-axis slide rail and can move on the X-axis slide rail, and the clamp is mounted on the Y-axis slide rail and can move on the Y-axis slide rail; The guide block is movably connected to the frame, and the guide block can move horizontally and rotate below the air blowing positive pressure block; The air blowing positive pressure block is movably connected to the frame, and the air blowing positive pressure block can move horizontally and / or rotate above the guide block; The air blowing positive pressure block can be connected to external gas through a pipeline; The porous plate comprises a grid, a filter membrane and a filtrate plate, wherein the filter membrane is connected to the bottom of the grid, the filtrate plate is detachably connected below the filter membrane, and the edges on both sides of the porous plate are turned outward to form a clamping position; One or more clamping strips are arranged at the bottom of the edge of the porous plate, and one or more clamping slots are arranged on the clamping jaws. When the clamping jaws are clamped at the clamping position of the porous plate, the clamping strips are matched and connected with the clamping slots in a one-to-one correspondence; A cut corner is provided on one edge of the porous plate to form a placement mark; There are multiple holes, and the multiple holes are arranged in multiple rows and columns; the holes are arch-shaped, T-shaped or triangular; The filter membrane has a filtration hole diameter of 5-50 microns; the filter membrane is welded on the edge of the bottom of the hole grid; Also included is a stacking assembly comprising multiple layers of placement locations capable of placing a multi-well plate.

2. A porous plate filtration method, characterized in that: The porous plate filtration device according to claim 1 comprises the following steps: Step 1: placing the porous plate into the stack assembly; Step 2: The clamping claw moves to the stacking assembly through the moving assembly and clamps the porous plate at the clamping position, and then moves to the guide block through the moving assembly and installs the porous plate on the guide block; Step 3: The guide block moves so that one side of the porous plate tilts upward; Step 4: Add the sample to the wells of the multi-well plate through an external sample adding device; Step 5: Start the positive air pressure block to blow air and apply pressure to the porous plate for filtering, and filter the holes on the porous plate in a single row one by one by moving the positive air pressure block or the guide block.

Images