Bat moth larvae age-specific feed

Abstract

The present invention belongs to the field of insect larvae breeding, and specifically relates to age-based feed for bat moth larvae. The present invention provides an age-based feed for bat moth larvae, comprising a Polygonum villosa extract and a bat moth larvae feed in a weight ratio of (1-5):100. The present invention prepares feed by treating the feed of bat moth larvae with a certain concentration of Polygonum villosa extract, so that the survival rate of the bat moth larvae that eat the feed is improved to a certain extent. The present invention provides a new idea for supplying bat moth larvae feed all year round.

Description

Technical Field

[0001] The invention belongs to the field of insect larvae breeding, and in particular relates to age-based feed for bat moth larvae. Background Art

[0002] Artificial propagation of the bat moth (Lepidoptera: Hepialidae), the host insect of the Cordyceps sinensis fungus, is an important part of the artificial cultivation of Cordyceps sinensis. More than 60 species of bat moth insects have been reported. Bat moths are holometabolous insects, and their life cycle includes egg, larva, pupa and adult stages. The most suitable soil for growth is alpine meadow and alpine shrub soil. Although the species of bat moths in different regions are different, there is a general regularity. From June to August each year, pupae emerge into adults. After mating, the female immediately lays eggs in nearby grass or shrub vegetation. Generally, each female moth lays 200-800 eggs. Bat moth larvae live in tunnels in natural soil, are omnivorous, and mainly feed on tender roots of plants; the larval stage is long, and generally it takes 900-1300 days of growth and development for 7-9 years to pupate, and there is an obvious alternation of generations; from October to April of the following year, bat moth larvae are dormant in the soil during the frozen soil period. In late May every year, bat moth larvae begin to form pupae, and after 1-2 months of the pupal stage, they emerge as adults.

[0003] In the natural environment, the completion of the entire generation cycle of bat moth insects is controlled by a variety of ecological factors such as species, geographical distribution, food, vegetation, soil structure, temperature, humidity and natural enemies. Among them, the food of the larvae is an important factor affecting their growth and survival rate. Summary of the invention

[0004] The present invention relates to a bat moth larvae age-divided feed. The inventor of the present invention creatively proposes to use a Polygonum villosa extract to prepare larval feed by studying the growth environment and living habits of the bat moth larvae, which not only improves the survival rate of the larvae, but also can use the Polygonum villosa extract to prepare larval feed in the season when fresh Polygonum villosa is lacking, so as to make up for the lack of fresh Polygonum villosa feed. In addition, the inventor observes larvae at different growth stages and specifically develops specific culture feed to meet the needs of larvae at different age stages.

[0005] Specifically, the present invention provides a bat moth larvae age-divided feed, comprising a Polygonum villosa extract and a bat moth larvae feed in a weight ratio of (1-5):100.

[0006] In some embodiments, the age-divided feed for 1-2 instar bat moth larvae contains the Polygonum villosa extract and the bat moth larvae feed in a weight ratio of (1-2):100.

[0007] In some embodiments, the age-divided feed for 3rd-4th instar bat moth larvae contains the Polygonum villosa extract and the bat moth larvae feed in a weight ratio of (2-5):100.

[0008] In some embodiments, the age-divided feed for bat moth larvae of the 5th instar and above contains the Polygonum villosa extract and the bat moth larvae feed in a total weight ratio of (5-10):100.

[0009] In some embodiments, the Polygonum villosa extract is obtained by adding 2-20 mL of water or 20-30% ethanol water to 1 gram of Polygonum villosa, boiling for 1-2 hours, filtering, and concentrating the filtrate.

[0010] In some embodiments, the Polygonum villosa extract is obtained by adding 10 mL of 20-30% ethanol water to 1 g of Polygonum villosa, boiling for 1-2 hours, filtering, and concentrating the filtrate.

[0011] In some embodiments, the bat moth larvae feed includes one or more combinations of carrots, Polygonum villosa, Potentilla anserina, and Potentilla hespermum.

[0012] In some embodiments, the bat moth larvae diet includes a combination of carrots and Polygonum villosa in a weight ratio of 1:1.

[0013] In some embodiments, the bat moth larvae feed is prepared by the following steps:

[0014] (1) washing the bat moth larvae feed, cutting it into pieces or strips, and airing it until the surface is dry;

[0015] (2) diluting the Polygonum villosa extract with an appropriate amount of water, spraying the extract onto the bat moth larvae feed processed in step (1), and stirring and mixing the bat moth larvae feed;

[0016] (3) The mixture treated in step (2) is allowed to stand at 5-10° C. for 1-3 hours to allow the surface of the dried bat moth larvae feed to fully absorb the Polygonum villosa extract until no water drips on the surface.

[0017] In another aspect, the present invention provides a method for preparing the age-based feed for the bat moth larvae of the present invention, comprising the steps of:

[0018] (1) washing the bat moth larvae feed, cutting it into pieces or strips, and airing it until the surface is dry;

[0019] (2) diluting the Polygonum villosa extract with an appropriate amount of water, spraying the extract onto the bat moth larvae feed processed in step (1), and stirring and mixing the bat moth larvae feed;

[0020] (3) The mixture treated in step (2) is allowed to stand at 5-10° C. for 1-3 hours to allow the surface of the dry bat moth larvae feed to fully absorb the Polygonum villosa extract until there is no water dripping on the surface.

[0021] The present invention prepares feed by processing the feed of bat moth larvae with a certain concentration of Polygonum villosa extract, so that the survival rate of the bat moth larvae eating the feed is improved to a certain extent. Polygonum villosa is a perennial herb, but it is not suitable for digging and picking at any time. The present invention provides a new idea for supplying larval feed all year round.

[0022] Detailed description

[0023] The invention provides an age-based feed for bat moth larvae, comprising a Polygonum villosa extract and a bat moth larvae feed in a weight ratio of (1-5):100.

[0024] Polygonum viviparum L. is a plant of the genus Polygonum in the family Polygonaceae. In some embodiments, the Polygonum viviparum described in the present invention is fresh Polygonum viviparum; in some embodiments, the Polygonum viviparum described in the present invention is dry Polygonum viviparum; in some embodiments, the Polygonum viviparum described in the present invention is its root, stem, leaf, fruit, whole herb or a combination thereof.

[0025] “The weight ratio of Polygonum aviculare extract and bat moth larvae feed (1-5):100” means that the weight ratio of Polygonum aviculare extract and bat moth larvae feed is 1-5:100, wherein the weight proportion of Polygonum aviculare extract is in the range of 1 to 5, including the endpoint values, and the weight proportion of bat moth larvae feed is 100.

[0026] The bat moth larvae feed refers to food suitable for bat moth larvae, including but not limited to Polygonum villosa, carrot, Potentilla anserina, Fern hemp, etc.

[0027] In some embodiments, the age-divided feed for 1-2 instar bat moth larvae contains the Polygonum villosa extract and the bat moth larvae feed in a weight ratio of (1-2):100.

[0028] In some embodiments, the age-divided feed for 1-2 instar bat moth larvae contains the extract of Polygonum villosa and the feed for bat moth larvae at a weight ratio of 1:100, 1.5:100, or 2:100.

[0029] In some embodiments, the age-divided feed for 3rd-4th instar bat moth larvae contains the Polygonum villosa extract and the bat moth larvae feed in a weight ratio of (2-5):100.

[0030] In some embodiments, the age-divided feed for 3rd-4th instar bat moth larvae contains the Polygonum villosa extract and the bat moth larvae feed in a weight ratio of 2:100, 2.5:100, 3:100, 3.5:100, 4:100, 4.5:100 or 5:100.

[0031] In some embodiments, the age-divided feed for bat moth larvae of the 5th instar and above contains the Polygonum villosa extract and the bat moth larvae feed in a total weight ratio of (5-10):100.

[0032] In some embodiments, the age-divided feed for bat moth larvae of the 5th instar and above is a bat moth larvae feed mixed with 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5% or 10% of the Polygonum aviculare extract in a weight ratio of 5:100, 5.5:100, 6:100, 6.5:100, 7:100, 7.5:100, 8:100, 8.5:100, 9:100, 9.5:100 or 10:100 of the Polygonum aviculare extract and the bat moth larvae feed.

[0033] In some embodiments, the Polygonum villosa extract is obtained by adding 2-20 mL of water or 20-30% ethanol water to 1 gram of Polygonum villosa, boiling for 1-2 hours, filtering, and concentrating the filtrate.

[0034] In some embodiments, the Polygonum villosa extract is obtained by adding 10-20 mL of water to 1 g of Polygonum villosa, boiling for 1-2 hours, filtering, and concentrating the filtrate.

[0035] In some embodiments, the Polygonum villosa extract is obtained by adding 10-20 mL of 20-30% ethanol water to 1 g of Polygonum villosa, boiling for 1-2 hours, filtering, and concentrating the filtrate.

[0036] In some embodiments, the Polygonum villosa extract is obtained by adding 10 mL of 20-30% ethanol water to 1 g of Polygonum villosa, boiling for 2 hours, filtering, and concentrating the filtrate.

[0037] In some embodiments, the bat moth larvae feed includes one or more combinations of carrots, Polygonum villosa, Potentilla anserina, and Potentilla hespermum.

[0038] In some embodiments, the bat moth larvae feed is carrots.

[0039] In some embodiments, the bat moth larvae feed is Polygonum villosa.

[0040] In some embodiments, the bat moth larvae diet is a combination of carrots and Polygonum villosa.

[0041] In some embodiments, the bat moth larvae diet includes a combination of carrots and Polygonum villosa in a weight ratio of 1:1.

[0042] In some embodiments, the bat moth larvae feed is prepared by the following steps:

[0043] (1) washing the bat moth larvae feed, cutting it into pieces or strips, and airing it until the surface is dry;

[0044] (2) diluting the Polygonum villosa extract with an appropriate amount of water, spraying the extract onto the bat moth larvae feed processed in step (1), and stirring and mixing the bat moth larvae feed;

[0045] (3) The mixture treated in step (2) is allowed to stand at 5-10° C. for 1-3 hours to allow the surface of the dried bat moth larvae feed to fully absorb the Polygonum villosa extract until no water drips on the surface.

[0046] Surface drying refers to sticking absorbent paper on the surface of the feed, with no watermark on the wet paper, and pressing it with fingers, the hardness is the same as before drying.

[0047] The extract of Polygonum villosa is diluted with an appropriate amount of water, wherein the amount of water is related to the amount of bat moth larvae feed, with the lower limit being that the extract can be evenly attached to the surface of the bat moth larvae feed, and the upper limit being that the extract can be fully absorbed by the surface of the bat moth larvae feed until there is no dripping phenomenon on the surface during the standing time in the next step.

[0048] The no-drip phenomenon refers to the situation that the solution or water does not flow down and gather into drops on the surface of the bat moth larvae feed due to gravity.

[0049] In another aspect, the present invention provides a method for preparing the age-based feed for the bat moth larvae of the present invention, comprising the steps of:

[0050] (1) washing the bat moth larvae feed, cutting it into pieces or strips, and airing it until the surface is dry;

[0051] (2) diluting the Polygonum villosa extract with an appropriate amount of water, spraying the extract onto the bat moth larvae feed processed in step (1), and stirring and mixing the bat moth larvae feed;

[0052] (3) The mixture treated in step (2) is allowed to stand at 5-10° C. for 1-3 hours to allow the surface of the dry bat moth larvae feed to fully absorb the Polygonum villosa extract until there is no water dripping on the surface. DETAILED DESCRIPTION

[0053] The embodiments of the present invention are described in detail below, wherein the same or similar reference numerals throughout represent the same or similar elements or elements with the same or similar functions. The embodiments described below are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0054] Example 1 Preparation of Polygonum villosa Extract

[0055] Group A: 500 g of chopped fresh Polygonum villosa was added to water at a ratio of 1 g / 10 mL, boiled for 2 hours, filtered, and the filtrate was concentrated to obtain 33.2 g of extract;

[0056] Group B: 500 g of chopped fresh Polygonum villosa was taken and boiled in 30% ethanol water at a ratio of 1 g / 10 mL for 1-2 hours, filtered, and the filtrate was concentrated to obtain 36.4 g of extract;

[0057] Group C: 500 g of chopped fresh Polygonum villosa was taken and boiled in 50% ethanol water at a ratio of 1 g / 10 mL for 1-2 hours, then filtered and the filtrate was concentrated to obtain 38.6 g of extract.

[0058] Group D: 500 g of chopped fresh Polygonum villosa was taken and boiled in 70% ethanol water at a ratio of 1 g / 10 mL for 1-2 hours, then filtered and the filtrate was concentrated to obtain 42.8 g of extract.

[0059] Example 2 Preparation of feed

[0060] Group 1: Cut fresh carrots and fresh Polygonum bulbilense into pieces, mix them in a weight ratio of 1:1, and set aside;

[0061] Group 2: Cut the fresh Polygonum bulbilense into pieces and set aside;

[0062] The third group: cut fresh carrots and fresh Polygonum villosa into pieces, mix them in a weight ratio of 1:1, and air-dry until the surface is dry; take the Polygonum villosa extract of group A at a weight ratio of 1.5g / 100g feed (fresh carrots and fresh Polygonum villosa), dilute with appropriate amount of water, spray on fresh carrots and fresh Polygonum villosa, stir to mix evenly, and let stand at 10°C for 2 hours to allow the dry surface of fresh carrots and fresh Polygonum villosa to fully absorb the Polygonum villosa extract without water dripping on the surface.

[0063] The fourth group: cut fresh carrots and fresh Polygonum villosa into pieces, mix them in a weight ratio of 1:1, and air-dry until the surface is dry; take the Polygonum villosa extract of group B at a weight ratio of 1.5g / 100g feed (fresh carrots and fresh Polygonum villosa), dilute with appropriate amount of water, spray on the fresh carrots and fresh Polygonum villosa, stir and mix evenly, and let stand at 10°C for 2 hours to allow the dry surfaces of the fresh carrots and fresh Polygonum villosa to fully absorb the Polygonum villosa extract without water dripping on the surface.

[0064] Group 5: Cut fresh carrots and fresh Polygonum villosa into pieces, mix them in a weight ratio of 1:1, and air-dry until the surface is dry. Take the Polygonum villosa extract of Group C at a weight ratio of 1.5g / 100g feed (fresh carrots and fresh Polygonum villosa), dilute with appropriate amount of water, spray on fresh carrots and fresh Polygonum villosa, stir and mix evenly, and let stand at 10°C for 2 hours to allow the dry surfaces of fresh carrots and fresh Polygonum villosa to fully absorb the Polygonum villosa extract without water dripping on the surface.

[0065] Group 6: Cut fresh carrots and fresh Polygonum villosa into pieces, mix them in a weight ratio of 1:1, and air-dry until the surface is dry; take the Polygonum villosa extract of Group D at a weight ratio of 1.5g / 100g feed (fresh carrots and fresh Polygonum villosa), dilute with appropriate amount of water, spray on fresh carrots and fresh Polygonum villosa, stir and mix evenly, and let stand at 10°C for 2 hours to allow the dry surfaces of fresh carrots and fresh Polygonum villosa to fully absorb the Polygonum villosa extract without water dripping on the surface.

[0066] Example 3: Rearing of 1-2-year-old bat moth larvae

[0067] A culture soil was formed by mixing 50% peat soil, 20% bran, 10% river sand, 10% small gravel and 10% wood ash, with a relative soil humidity of 40RH%; the ambient temperature was 10°C, and the humidity was 70-75RH%. The feed prepared in Example 2 was used to feed 1-2 age bat moth larvae for 1 month. The number of survivors was counted and the survival rate was calculated. The survival rate = the number of surviving bat moth larvae after one month of feeding / the number of bat moth larvae initially fed.

[0068] Table 1 Effects of different feeds on the survival rate of bat moth larvae

[0069]

[0070] Example 4: Rearing of bat moth larvae by age

[0071] Fresh carrots and fresh Polygonum villosa were cut into pieces, mixed in a weight ratio of 1:1, and air-dried until the surface was dry. The pieces were divided into three groups, I, II and III. The Polygonum villosa extract of group B was taken at a weight ratio of 2g / 100g, 3g / 100g and 7g / 100g feed (fresh carrots and fresh Polygonum villosa) respectively. The extract was diluted with an appropriate amount of water and sprayed on the fresh carrots and fresh Polygonum villosa of I, II and III respectively. The mixture was stirred to mix evenly and allowed to stand at 10°C for 2 hours to allow the dry surfaces of the fresh carrots and fresh Polygonum villosa to fully absorb the Polygonum villosa extract and there was no water dripping on the surface.

[0072] The culture soil is composed of a mixture of 50% peat soil, 20% bran, 10% river sand, 10% small gravel and 10% wood ash, with a soil relative humidity of 40RH%; the ambient temperature is 10°C, and the humidity is 70-75RH%. The above feed is used to feed 1-2-year-old, 3-4-year-old and 5-year-old or above bat moth larvae for 1 month. The surviving number is counted and the survival rate is calculated. The survival rate = the number of surviving bat moth larvae after 1 month of feeding / the number of bat moth larvae initially fed.

[0073] Table 2 Effects of different feeds on the survival rate of bat moth larvae of different ages

[0074]

[0075] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0076] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A bat moth larvae age-based feed, characterized in that: Containing a Polygonum villosa extract and a bat moth larvae feed in a weight ratio of (1-5):100; The Polygonum villosa extract is prepared by adding 10 mL of 20-30% ethanol water to 1 g of Polygonum villosa, boiling for 1-2 hours, filtering, and concentrating the filtrate; The bat moth larvae feed comprises one or more combinations of carrots, Polygonum bulbiferum, and Potentilla anserina; The preparation of the age-based feed for bat moth larvae comprises the following steps: (1) washing the bat moth larvae feed, cutting it into pieces or strips, and airing it until the surface is dry; (2) diluting the Polygonum villosa extract with an appropriate amount of water, spraying the extract onto the bat moth larvae feed processed in step (1), and stirring to mix the bat moth larvae feed; (3) The mixture treated in step (2) is allowed to stand at 5-10°C for 1-3 hours to allow the surface of the dry bat moth larvae feed to fully absorb the Polygonum villosa extract until no water drips on the surface.

2. The age-based feed for bat moth larvae according to claim 1, characterized in that: The bat moth larvae feed comprises carrots and Polygonum villosa in a weight ratio of 1:

1.

3. A feed for 1-2-year-old bat moth larvae according to age, characterized in that: Containing a Polygonum villosa extract and a bat moth larvae feed in a weight ratio of (1-2):100; The Polygonum villosa extract is prepared by adding 10 mL of 20-30% ethanol water to 1 g of Polygonum villosa, boiling for 1-2 hours, filtering, and concentrating the filtrate; The bat moth larvae feed comprises one or more combinations of carrots, Polygonum bulbiferum, and Potentilla anserina; The preparation of the age-based feed for 1-2-year-old bat moth larvae comprises the following steps: (1) washing the bat moth larvae feed, cutting it into pieces or strips, and airing it until the surface is dry; (2) diluting the Polygonum villosa extract with an appropriate amount of water, spraying the extract onto the bat moth larvae feed processed in step (1), and stirring to mix the bat moth larvae feed; (3) The mixture treated in step (2) is allowed to stand at 5-10°C for 1-3 hours to allow the surface of the dry bat moth larvae feed to fully absorb the Polygonum villosa extract until no water drips on the surface.

4. A 3-4-year-old bat moth larvae age-based feed, characterized in that: Containing a Polygonum villosa extract and a bat moth larvae feed in a weight ratio of (2-5):100; The Polygonum villosa extract is prepared by adding 10 mL of 20-30% ethanol water to 1 g of Polygonum villosa, boiling for 1-2 hours, filtering, and concentrating the filtrate; The bat moth larvae feed comprises one or more combinations of carrots, Polygonum bulbiferum, and Potentilla anserina; The preparation of the age-based feed for 3-4-instar bat moth larvae comprises the following steps: (1) washing the bat moth larvae feed, cutting it into pieces or strips, and airing it until the surface is dry; (2) diluting the Polygonum villosa extract with an appropriate amount of water, spraying the extract onto the bat moth larvae feed processed in step (1), and stirring to mix the bat moth larvae feed; (3) The mixture treated in step (2) is allowed to stand at 5-10°C for 1-3 hours to allow the surface of the dry bat moth larvae feed to fully absorb the Polygonum villosa extract until no water drips on the surface.

5. An age-based feed for bat moth larvae of age 5 and above, characterized in that: Containing a Polygonum villosa extract and a bat moth larvae feed in a weight ratio of (5-10):100; The Polygonum villosa extract is prepared by adding 10 mL of 20-30% ethanol water to 1 g of Polygonum villosa, boiling for 1-2 hours, filtering, and concentrating the filtrate; The bat moth larvae feed comprises one or more combinations of carrots, Polygonum bulbiferum, and Potentilla anserina; The preparation of the age-based feed for bat moth larvae of age 5 and above comprises the following steps: (1) washing the bat moth larvae feed, cutting it into pieces or strips, and airing it until the surface is dry; (2) diluting the Polygonum villosa extract with an appropriate amount of water, spraying the extract onto the bat moth larvae feed processed in step (1), and stirring to mix the bat moth larvae feed; (3) The mixture treated in step (2) is allowed to stand at 5-10°C for 1-3 hours to allow the surface of the dry bat moth larvae feed to fully absorb the Polygonum villosa extract until no water drips on the surface.

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