A microbial sample pretreatment system

Abstract

The present invention relates to a microbial sample pretreatment system, comprising a workbench, wherein a sampling slot and a through slot are respectively provided on both sides of a sterilizing device on the workbench, and a line connecting the center of the sampling slot and the center of the sterilizing device is perpendicular to the length direction of the through slot and passes through the midpoint of the through slot length; a clamping mechanism capable of controlling the clamping of a sample tube is installed above the sampling slot; a marking device comprises an XZ biaxial linear module, an inoculation loop is installed on the slider of the Z-axis linear module, and a suction cup driven by the Y-axis linear module is provided below the workbench; an untreated culture dish bin comprises a top plate and a bottom plate fixed by connecting columns, and two supporting plates are fixed to the bottom plate below each arc-shaped lower notch by a connecting plate, and a side of the connecting plate connecting the arc-shaped lower notch and the supporting plate is configured as an arc-shaped inclined surface. The present invention utilizes fully mechanized operation to simulate manual separation of microorganisms, thereby reducing the risk of infection and inaccurate results during manual operation, improving work efficiency, and reducing labor costs.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a microbial sample pretreatment system. Background Art

[0002] The statements herein merely provide background information related to the present invention and do not necessarily constitute prior art.

[0003] Clinical microbial isolation is the most fundamental and crucial task for medical institutions in areas such as disease diagnosis, rational use of antibiotics, and hospital infection control. Currently, manual three-zone or four-zone demarcation is often used for separation. This method is slow, and manual labor inevitably brings the microbial samples into close, direct contact during the separation process, placing the individual at risk of infection during the inoculation process. Furthermore, the rapid changes in the external environment and increased population density have led to an increase in the number of clinical samples in hospitals, significantly increasing the workload of staff. Furthermore, manual operations are time-consuming, and the processing results vary depending on staff proficiency and operational standardization, directly impacting the accuracy of subsequent test results. Therefore, a fully automated microbial sample processing system with standardized processing capabilities is particularly important to the market. Summary of the Invention

[0004] In response to the shortcomings of the existing technology, the present invention provides a microbial sample pretreatment system that uses fully mechanized operations to simulate manual separation of microorganisms, which can reduce the risks of infection and inaccurate results during manual operations, improve work efficiency, and reduce labor costs.

[0005] The present invention is achieved through the following technical solutions:

[0006] Provided is a microbial sample pretreatment system, comprising a workbench, an untreated culture dish compartment, and a controller. The workbench is equipped with a sample oscillating device, a sample tube clamping and lid opening and closing device, a sterilizing device, a streaking device, a plate pushing and lid opening and closing device, an automatic labeling device, and a culture dish storage device, all controlled by the controller. A sampling slot and a through slot are provided on either side of the sterilizing device, respectively. A line connecting the center of the sampling slot and the center of the sterilizing device is perpendicular to the length of the through slot and passes through the midpoint of the through slot. A clamping mechanism for controlling the clamping of the sample tube is installed above the sampling slot.

[0007] The scribing device includes an XZ biaxial linear module mounted above the workbench. A controllable inoculation loop is mounted on the slider of the Z-axis linear module. A Y-axis linear module, positioned along the length of the slot, is mounted directly below the workbench. A suction cup, movable within the slot, is mounted on the slider of the Y-axis linear module and connected to a vacuum pump via an air pipe.

[0008] The unprocessed culture dish bin includes a top plate and a bottom plate fixed by connecting columns. The top plate is concavely provided with a plurality of arc-shaped upper notches from the edge of the top plate to the center of the top plate. The bottom plate is provided with arc-shaped lower notches that are opposite to the arc-shaped upper notches one by one. Two supporting plates are fixed to the bottom plate through a connecting plate below each arc-shaped lower notch. A slide gap for taking and placing culture dishes is left between the two supporting plates and the connecting plate. One side of the connecting plate connecting the arc-shaped lower notch and the supporting plate is set as an arc-shaped inclined surface. The bottom plate and the top plate form an open placement bin between the supporting plate, the arc-shaped upper notch and the arc-shaped lower notch.

[0009] Furthermore, a first rotating motor is installed on the slider of the Y-axis linear module, and the suction cup is installed on the output shaft of the first rotating motor. A cavity is provided in the output shaft and a through hole communicating with the outside world is opened. One end of the air pipe enters the cavity through the through hole and is connected to the suction cup.

[0010] By installing a first rotary motor on the slider of the Y-axis linear module, the first rotary motor is used to drive the suction cup to rotate and then drive the culture dish to rotate, and multiple zone lines of the culture dish can be achieved in conjunction with an inoculation loop.

[0011] Furthermore, a second rotating motor with an output shaft arranged along the Y-axis direction is installed on the slider of the Z-axis linear module. The output shaft of the second rotating motor is connected to at least one inoculation ring through a mounting block. The middle part of the inoculation ring is fixed on the mounting block, and the two ends of the inoculation ring are respectively provided with a marking end.

[0012] By setting up a second rotating motor, the output shaft of the second rotating motor is used to rotate the inoculating loop, and the inoculation and marking are achieved in conjunction with the marking ends at both ends. Multiple inoculation loops can be set up, and in the case of multiple sampling, different marking ends of the inoculation loop can be selected for inoculation and marking according to different sampling sources.

[0013] Furthermore, it also includes a classification and recovery device controlled by a controller, the classification and recovery device includes a barcode recognition system and a data storage system, and a barcode scanner installed on the workbench facing the sampling slot and electrically connected to the barcode recognition system; the workbench is provided with two open recovery bins below the sampling slot, and a classification guide plate is installed above the two recovery bins and rotated by a third rotating motor.

[0014] The sample tubes can be affixed with different QR codes or barcodes, or not affixed. The barcodes on the sample tubes can be scanned and identified by the barcode scanner on the workbench. The rotation direction of the classification guide plate is controlled according to the scanning and identification results, so that the classification guide plate can guide different sample tubes into different recovery bins by adjusting the rotation direction, thereby realizing the classified recovery of the sample tubes.

[0015] Furthermore, the sample tube clamping and lid opening device includes a motion mechanism and an operating mechanism. The motion mechanism includes an XYZ three-axis linear module electrically connected to the controller; the operating mechanism includes a fixed rod, one end of which is provided with at least two clamping plates located on the same circle, the diameter of the circle is smaller than the diameter of the sample tube cover, the middle parts of the two clamping plates are rotatably connected to one end of the fixed rod, and the upper parts of the two clamping plates are respectively connected to the fixed rod through springs.

[0016] The clamping pieces of the operating mechanism cooperate with each other and cooperate with the spring. When they move above the sample tube and move downward, the tube cover of the sample tube can be put into the clamping piece by relying on pressure. The sample tube can be clamped by the squeezing force. The position of the operating mechanism to grab the sample tube can be adjusted by the XYZ three-axis linear module drive, so that the position can be adjusted according to the predetermined program. It is easy to operate, simple in structure, low in failure rate and stable in performance.

[0017] Furthermore, the other end of the fixing rod is connected to a fourth rotary motor, and the fourth rotary motor is mounted on a slider of the Z-axis linear module.

[0018] By installing a rotary motor on the slider of the Z-axis linear module and connecting it to an operating mechanism, the motor drives the operating mechanism to rotate, enabling the opening and closing of the lid. To open the lid, the motor rotates counterclockwise to unscrew the sample tube cap. To close the lid, the motion mechanism drives the clamping piece to press the lid against the tube mouth. The motor rotates clockwise to tighten the tube cap. The motion mechanism then controls the operating mechanism to move upward, using the upward force to disengage the spring-loaded clamping piece from the sample tube cap.

[0019] Furthermore, the sample tube clamping and lid opening device also includes a photographing device installed on the Z-axis linear module slider and used to identify the color of the sample tube cap.

[0020] The color of the sample cover can include the sample type, usage date, sample number and patient information, etc. The sample cover is photographed or scanned using a photographing device to obtain an image, and the obtained image information is identified, which can be used by the controller to control the next opening operation; if it is identified as an unknown sample, the guidance system will not open the cover of this sample and will proceed directly to the next operation.

[0021] Furthermore, the sample shaking device includes a shaker installed in the workbench, and a sample tray with a handle is detachably connected to the shaker. The sample tray is provided with a plurality of tube holes that match the sizes of the sample tubes.

[0022] By placing the sample tube to be tested in the tube hole in the sample tray, when the instrument is started, the sample in the sample tube cooperates with the shaking effect of the shaker to promote the homogenization of the sample and improve the detection rate of the sample.

[0023] Furthermore, the culture dish storage device is detachably connected to the workbench, and includes a processed culture dish bin and a lifting mechanism. The processed culture dish bin includes an upper splint and a lower splint fixed by a connecting column. The upper splint is circumferentially recessed from the edge to the center to form a plurality of arc-shaped upper notches. The lower splint is formed with arc-shaped lower notches that are opposite to the arc-shaped upper notches one by one. The lower splint and the upper splint form an open storage bin between the arc-shaped upper notches and the arc-shaped lower notches. The lower splint is hinged with a one-way limit plate that can only rotate upward at the edge of each arc-shaped lower notch. The upper splint is provided with a limit switch for detecting the position of the culture dish at the edge of each arc-shaped upper notch. The center of the lower splint is connected to a fifth rotating motor electrically connected to the limiter; the lifting mechanism is arranged between the outlet of the automatic labeling device and the processed culture dish bin, and the lifting mechanism includes a lifting plate matching the arc-shaped lower notch, and a lifting cylinder for driving the lifting plate to move up and down.

[0024] When the culture dish is labeled after marking, the culture dish pushing device pushes the culture dish to the storage port of the processed culture dish bin, and uses the lifting cylinder of the lifting mechanism to drive the lifting plate to lift the culture dish upward and rotate the one-way limit plate to drag the lifted culture dish, and then the lifting plate falls back and lifts the next labeled culture dish. The operation is repeated. After the limiter recognizes the culture dish, it indicates that the single storage bin is full of culture dishes. At this time, the limiter sends a signal to the fifth drive motor, and the controller controls the fifth rotary motor to drive the whole to rotate a certain angle so that the other storage port of the processed culture dish bin is facing the labeled culture dish.

[0025] Furthermore, it also includes a shell, a touch screen electrically connected to the controller is provided on the outside of the shell, and an emergency stop button, a reset button, a stop button, a start button and a switch button are provided on the display screen, an ultraviolet lamp, a lighting lamp and an air purifier are installed in the shell, and an exhaust fan is installed on one side of the shell.

[0026] The housing encloses the workbench, isolating it from the outside world and preventing harmful inhalation of volatile aerosols generated during the inoculation process. An internal air purifier removes dust, odor, smoke, and bacteria, purifying the internal environment. Combined with UV and incandescent lighting, it also provides strong sterilization. An exhaust fan on the side of the housing removes dust particles and bacteria from the instrument. A touchscreen display on the housing displays the entire machine's operating status and facilitates operation with multiple switches.

[0027] Beneficial effects of the present invention:

[0028] The efficient microbial sample pretreatment system uses a linear module to replace the traditional manual sample processing and streak inoculation. It automatically performs a series of operations on microbial samples, including scanning codes, opening lids, screwing lids, streak inoculation, classification and recycling, and labeling. This can greatly ensure the biosafety of microbiological workers while improving work efficiency.

[0029] Since the present invention uses a linear module to replace the robot arm operation, the failure rate is reduced and the work efficiency is improved. In addition, by setting the sampling slot center, the sterilization device center and the midpoint of the through slot length to be collinear, the inoculation, sterilization and marking operations can be completed in one direction during operation, which can be completed only through the linear motion of the XZ biaxial linear module, which greatly simplifies the operation steps, improves the stability of the operation, and makes the overall operation more efficient. The XZ biaxial linear module above the inoculation table is used to drive the displacement of the inoculation ring, and the Y-axis linear module below the inoculation table cooperates with the suction cup to drive the displacement of the culture dish. It can realize various marking forms such as Feng-shaped marking and spiral marking according to actual needs, and can cooperate with the first drive motor to realize multi-zone marking. It has a high degree of automation, reduces the labor intensity of manual marking, has high marking efficiency, good marking effect and high safety.

[0030] The connecting buckles arranged under the bottom plate of the unprocessed culture dish bin can be movably connected to the workbench and can rotate with the motor. The top plate and the bottom plate form an open culture dish placement bin between the arc-shaped upper notch, the arc-shaped lower notch and the support plate, which can be used to stack culture dishes therein and place them on the support plate. Due to the arc-shaped inclined surface arranged on the connecting plate on the support plate, the bottommost stacked culture dish can naturally protrude from the whole by gravity, effectively preventing jamming between culture dishes, making it easier to manually pick up culture dishes or for the slide module to pick up and use new culture dishes through the slide gap. The structure is simple, the effect is obvious, complex mechanical structure is eliminated, the stability is high, and the efficiency of taking culture dishes is greatly improved.

[0031] The classification and recovery device of the present invention can quickly determine the sample type. Unrecognizable sample tubes are guided by a classification guide plate to fall into a recovery bin for unknown samples. After sampling, sample tubes identified by a barcode scanner are guided by a classification guide plate to fall into a recovery bin for identified samples. Automatically guiding sample tubes into their corresponding recovery bins prevents confusion and other issues. Simple to operate and highly efficient, it solves the problem of automatic sample identification and recovery in microbial sample pretreatment systems, making it suitable for a wide range of applications.

[0032] Furthermore, the present invention allows for the opening of the tube cap prior to automatic sampling and inoculation of identified samples by means of an operating mechanism in conjunction with a clamping mechanism above the sampling slot and a fourth rotary motor. After sampling, the cap is tightened by a reverse operation, automating and facilitating the entire operation. This invention offers simple operation, high efficiency, reduced costs, avoids sample mix-up, and ensures accurate and stable experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0034] Figure 2 for Figure 1 A top view of

[0035] Figure 3 Schematic diagram of the structure of the scribing device in the present invention;

[0036] Figure 4 Schematic diagram of the structure of the recycling and classification device in the present invention;

[0037] Figure 5 Schematic diagram of the structure of the operating mechanism of the sample tube clamping and cover opening device of the present invention;

[0038] Figure 6 This is a schematic diagram of the overall structure of the untreated culture dish bin in the present invention;

[0039] Figure 7 for Figure 6 A top view of

[0040] Figure 8 Schematic diagram of the connection between the connecting plate, the bottom plate and the supporting plate in the untreated culture dish bin;

[0041] Figure 9 This is a top view of the installation of the culture dish storage device of the present invention.

[0042] As shown in the figure:

[0043] 1. Workbench, 2. Sample oscillating device, 3. XYZ three-axis linear module, 4. Fourth rotary motor, 5. Sampling slot, 6. Sterilization device, 7. Through slot, 8. X-axis linear module, 9. Z-axis linear module, 10. Conveyor belt, 11. Label printer, 12. Culture dish storage device, 13. Unprocessed culture dish compartment, 14. Sample tube, 15. Clamping mechanism, 16. Culture dish, 17. Suction cup, 18. Marking end, 19. First rotary motor, 20. Y-axis linear module, 21. Vacuum pump, 22. Second rotary motor, 23. Inoculation loop, 24. Operation Mechanism, 25. Controller, 26. Barcode scanner, 27. Third rotating motor, 28. Classification guide plate, 29. Slide, 30. Recovery bin, 31. Photographing device, 32. Fixing rod, 33. Clamping piece, 34. Chamfer, 35. Tube cover, 36. Spring, 37. Top plate, 38. Arc-shaped upper notch, 39. Arc-shaped lower notch, 40. Connecting plate, 41. Slide gap, 42. Support plate, 43. Arc-shaped inclined plane, 44. Bottom plate, 45. Connecting column, 46. Handle, 47. Lifting plate, 48. Upper clamping plate, 49. One-way limit plate, 50. Culture dish flipping mechanism. DETAILED DESCRIPTION

[0044] In order to clearly illustrate the technical features of this solution, this solution is described below through specific implementation methods.

[0045] A microbial sample pretreatment system includes a workbench 1, an untreated culture dish compartment 13, and a controller 25. The workbench 1 is provided with a sample shaking device 2, a sample tube clamping and lid opening and closing device, a sterilizing device 6, a streaking device, a culture dish pushing and lid opening and closing device, an automatic labeling device, and a culture dish storage device 12, all of which are controlled by the controller 25. In this embodiment, the sterilizing device 6 is an infrared inoculating loop sterilizer.

[0046] Among them: the plate pushing and lid opening and closing device and the automatic labeling device are both existing technologies, and a brief introduction is given here. The plate pushing and lid opening and closing device is used in the microbial sample pretreatment system, which flips the inverted culture plate and places it on the workbench. The plate pushing and lid opening and closing device is located on the outlet side of the untreated culture plate bin, and has a culture plate flipping mechanism driven by a motor. The culture plate flipping mechanism is provided with a suction cup that can suck the culture plate or exhaust it off; the culture plate flipping mechanism sucks the bottom of the inverted culture plate pushed out from the outlet side of the untreated culture plate bin and flips it 180° and then places it upright on the workbench. The suction cup is connected through the Y-axis linear module to move the plate to be inoculated to the midpoint of the through groove. After inoculation, the culture plate flipping mechanism sucks the bottom of the culture plate 16 and flips it 180° to return it to its original position and close the cover.

[0047] The automatic labeling device includes a label adsorption panel, a vertical conveying mechanism and a horizontal conveying mechanism. The label adsorption panel is controlled by the vertical conveying mechanism and the horizontal conveying mechanism to achieve lifting and left and right movement to adsorb the label at the outlet of the label printer 11 and move it to the labeling area to be attached to the bottom of the culture dish 16; the label adsorption panel is provided with air holes, and the label adsorption panel is connected to the suction end and the outlet end of the vacuum pump 21.

[0048] A sampling slot 5 and a through slot 7 are respectively provided on both sides of the sterilization device 6 on the workbench 1, and the line connecting the center of the sampling slot 5 and the center of the sterilization device 6 is perpendicular to the length direction of the through slot 7 and passes through the midpoint of the length of the through slot 7. A clamping mechanism 15 for controlling the clamping of the sample tube is installed above the sampling slot 5. The clamping mechanism 15 is a conventional existing technology and can utilize two to set multiple clamping blocks, which are driven by a cylinder. When the piston rod of the cylinder is extended, the clamping blocks approach each other to clamp the sample tube 14, and when the piston rod is retracted, the clamping blocks move away from each other to release the clamping of the sample tube 14.

[0049] The marking device includes an XZ biaxial linear module arranged above the workbench 1, wherein: a controllable rotatable inoculation ring 23 is installed on the slider of the Z-axis linear module 9, and a Y-axis linear module 20 is installed below the workbench 1 directly below the through groove 7 and arranged along the length direction of the through groove 7. A suction cup 17 that can move in the through groove 7 is installed on the slider of the Y-axis linear module 20, and the suction cup 17 is connected to the vacuum pump 21 through an air pipe.

[0050] A first rotating motor 19 is installed on the slider of the Y-axis linear module 20, and a suction cup 17 is installed on the output shaft of the first rotating motor 19. A cavity is provided in the output shaft and a through hole communicating with the outside world is opened. One end of the air pipe enters the cavity through the through hole and is connected to the suction cup 17.

[0051] A second rotating motor 22 with an output shaft arranged along the Y-axis direction is installed on the slider of the Z-axis linear module 9. The output shaft of the second rotating motor 22 is connected to at least one inoculation ring 23 through a mounting block. The middle part of the inoculation ring 23 is fixed on the mounting block, and the two ends of the inoculation ring 23 are respectively provided with a marking end 18.

[0052] The unprocessed culture dish bin 13 comprises a top plate 37 and a bottom plate 44 secured by connecting posts 45. A connecting clip is mounted in the center of the bottom surface of the bottom plate 44, allowing for easy installation and removal from the workbench. The connecting clip serves as a secure connection, facilitating automated handling for the placement and removal of the culture dishes 16. A handle 46 is mounted in the center of the top surface of the top plate 37 for easy handling, transport, and movement of the entire culture dish bin.

[0053] The top plate 37 is formed with a plurality of arc-shaped upper notches 38 from the edge of the top plate to the center of the top plate. The bottom plate 44 is formed with arc-shaped lower notches 39 that are directly opposite to the arc-shaped upper notches 38. Two supporting plates 42 are fixed to the bottom plate 44 through a connecting plate 40 below each arc-shaped lower notch 39. The supporting plates 42 are set 1 to 3 cm below the bottom plate 44 to accommodate the lowest culture dish 16 and facilitate the removal of the culture dish 16. A slide gap 41 for removing and placing the culture dish 16 is left between the two supporting plates 42 and the connecting plate 40. The side of the connecting plate 40 connecting the arc-shaped lower notch 39 and the supporting plate 42 is set as an arc-shaped inclined surface 43, as shown in FIG. Figure 8 As shown, one side of the connecting plate 40 connecting the arc-shaped lower notch 39 and the supporting plate 42 is set as an arc-shaped inclined surface 43. The arc-shaped inclined surface 43 is inclined from the edge of the arc-shaped lower notch toward the supporting plate 42 and forms an angle of 40° to 70° with the plane of the supporting plate 42. In this embodiment, the angle between the arc-shaped inclined surface 43 and the supporting plate 42 is 45°. Figure 8 As shown at angle α in the middle, the bottom plate 44 and the top plate 37 form an open storage bin between the support plate 42, the arcuate upper notch 38, and the arcuate lower notch 39. At least two connecting posts 45 are connected between the top plate 37 and the bottom plate 44 at the edge of each storage bin. The retaining effect of the connecting posts 45 allows multiple culture dishes 16 to be stacked and placed in the storage bin without displacement, helping to maintain the stability of the culture dishes within the bin.

[0054] Among them: a slide gap 41 is left between the two supporting plates 42 and the connecting plate 40 for easy removal of culture dishes. The slide gap 41 can provide a working space for the culture dish pushing device, and the adsorption mechanism is used to remove the lowest culture dish 16 from the slide gap 41, thereby ensuring the convenience of taking and placing the culture dish 16.

[0055] The processing system of the present invention also includes a sorting and recovery device controlled by a controller 25. The sorting and recovery device includes a barcode recognition system and a data storage system, as well as a barcode scanner 26 mounted on the workbench 1, facing the sampling slot 5 and electrically connected to the barcode recognition system. The workbench 1 is provided with two open recovery bins 30 below the sampling slot 5. Sorting guide plates 28 are mounted above the two recovery bins 30, which are rotated by a third rotary motor 27. To ensure that the recovered sample tubes 14 do not fall out of the recovery bins 30, a slide 29 is connected upwardly to the open end of each recovery bin 30, which is compatible with the sorting guide plates 28.

[0056] The sample tube clamping and lid opening and closing device includes a motion mechanism and an operating mechanism 24. The motion mechanism includes an XYZ three-axis linear module 3 electrically connected to a controller 25. The operating mechanism 24 includes a fixed rod 32. One end of the fixed rod 32 is provided with at least two clamping pieces 33 located on the same circle. The diameter of the circle is smaller than the diameter of the sample tube cover 35. The middle parts of the two clamping pieces 33 are rotatably connected to one end of the fixed rod 32. The upper parts of the two clamping pieces 33 are respectively connected to the fixed rod 32 through springs 36. In order to better achieve the clamping effect, three clamping pieces 33 are generally set at the end of the fixed rod 32. The three clamping pieces 33 are located on the same circle, and the angle between them is 60°. The diameter of the circle is smaller than the diameter of the sample tube cover 35, which makes it convenient to clamp the sample tube 14 by downward pressure. Specifically, the cover of the sample tube 14 slides into the three clamping pieces 33 of the inverted triangular chamfer 34 under the action of pressure, and the clamping pieces 33 rotate around the axis and stretch open. The spring 36 can continuously provide clamping force, and the sample tube 14 can be clamped under the elastic force of the spring 36.

[0057] The other end of the fixing rod 32 is connected to the fourth rotary motor 4 , which is mounted on a slider of the Z-axis linear module in the XYZ three-axis linear module.

[0058] The sample tube clamping and lid opening and closing device further includes a photographing device 31 mounted on the Z-axis linear module slider and used to identify the color of the sample tube cap.

[0059] The sample shaking device 2 includes a shaker installed in the workbench 1. A sample tray with a handle is detachably connected to the shaker. The sample tray is provided with a plurality of tube holes that match the sizes of the sample tubes.

[0060] The culture dish storage device 12 is detachably connected to the workbench 1, and includes a processed culture dish bin and a lifting mechanism. The processed culture dish bin includes an upper splint 48 and a lower splint fixed by connecting columns. The upper splint is concave from the edge to the center to form a plurality of arc-shaped upper notches. The lower splint is formed with arc-shaped lower notches that are opposite to the arc-shaped upper notches one by one. The lower splint and the upper splint 48 form an open storage bin between the arc-shaped upper notches and the arc-shaped lower notches. The lower splint is hinged with a one-way limit plate 49 that can only rotate upward at the edge of each arc-shaped lower notch, and the center of the lower splint is connected to a fifth rotating motor; the lifting mechanism is arranged between the outlet of the automatic labeling device and the processed culture dish bin, and the lifting mechanism includes a lifting plate 47 matching the arc-shaped lower notch, and a lifting cylinder that drives the lifting plate 47 to move up and down.

[0061] The processing system of the present invention also includes a shell, a touch screen electrically connected to the controller is provided on the outside of the shell, and an emergency stop button, a reset button, a stop button, a start button and a switch button are provided on the screen. An ultraviolet lamp, a lighting lamp and an air purifier are installed in the shell, and an exhaust fan is installed on one side of the shell.

[0062] The workflow of the present invention:

[0063] The culture dishes 16 of the same specifications or different specifications are inverted and placed in different placement bins in the unprocessed culture dish bin 13 in sequence. The culture dishes in the placement bins are stacked vertically in sequence. Generally, the specifications and models of the culture dishes in one placement bin are the same. Of course, culture dishes of different specifications can also be mixed and used according to actual needs. Since the edge of the placement bin is connected with a connecting column 45 between the top plate 37 and the bottom plate 44, the connecting column can limit the vertical culture dishes to prevent them from tipping over and causing contamination of the culture dishes. Since the connecting plate 40 connecting the supporting plate 42 and the arc-shaped lower notch 39 is provided with a slope structure, the culture dish placed at the bottom protrudes from the entire structure. The suction cup of the flat dish pushing device is driven by the driving device to enter the slide gap 41 between the supporting plates 42, and after the culture dish is adsorbed, it slides out in the opposite direction and sends the inverted culture dish into the culture dish flipping mechanism of the above-mentioned flat dish pushing and lid opening and buckling device. After the lid is flipped open by the culture dish flipping mechanism, it is sent into the through groove 7 for inoculation and marking.

[0064] At the same time, different sample tubes are placed in the sample oscillating device 2 on one side of the workbench 1. The sample tube clamping and lid opening device clamps a sample tube 14 through the clamping piece 33 of the operating structure and moves it to the top of the sampling slot 5. The fourth rotary motor 4 rotates and drives the sample tube 14 to rotate 360 ​​degrees. The barcode scanner 26 of the recycling and sorting device scans and recognizes the barcode on the sample tube and recognizes the acquired image information. After the recognition is completed, the Z-axis linear module drives the operating mechanism 24 to send the sample tube into the sampling slot 5. The sample tube is clamped by the clamping mechanism 15, and the operating mechanism 24 separates from the sample tube upward.

[0065] Determine the recovery location of the sample tube based on the identification results:

[0066] If the sample is unknown, no sampling is performed. The third rotary motor 27 is started to rotate the classification guide plate 28 to guide it to the recovery bin 30 for recovering unknown samples. Then the clamping mechanism 15 above the sampling slot 5 is opened, and the sample tube 14 slides through the classification guide plate 28 and the slide 29 to the recovery bin 30 for unknown samples.

[0067] If it is an identification sample, the corresponding inoculation and streaking method will be automatically controlled according to the identified sample information:

[0068] Before inoculation and marking, the Z-axis linear module drives the operating mechanism to use the clamping piece 33 to grab the sample tube 14 downward, and at the same time the fourth rotary motor 4 is started to open the tube cover 35 through the rotation action to facilitate the inoculation loop 23 to take samples.

[0069] Take the Feng character line and the multi-area line as examples to illustrate.

[0070] The operation of the Feng character line includes the following steps:

[0071] The following steps are involved:

[0072] a1. Sterilizing the inoculating ring: The X-axis linear module 8 drives the inoculating ring 23 to move forward to the top of the high-temperature electromagnetic sterilizer 6. The second rotary motor 22 rotates to align the inoculating ring 23 vertically. The Z-axis linear module 9 drives the inoculating ring 23 forward to descend and extend into the high-temperature electromagnetic sterilizer 6. After sterilization, the Z-axis linear module 9 drives the inoculating ring 23 in the reverse direction to ascend and leave the high-temperature electromagnetic sterilizer 6.

[0073] a2. Dipping the sample: The X-axis linear module 8 drives the sterilized inoculating loop 23 forward to move it to the top of the sampling slot 5. The Z-axis linear module 9 drives the inoculating loop 23 forward to descend and extend it into the sample tube 14. After the marking end 18 dips the sample, the Z-axis linear module 9 drives the inoculating loop 23 backward to ascend and leave the sample tube. The second rotary motor 22 rotates to tilt the inoculating loop 23.

[0074] a3. Marking the culture dish: The suction cup 17 sucks the culture dish 16 through the vacuum pump 21, and the Y-axis linear module 20 drives the suction cup 17 and the culture dish 16 to move to the middle point of the through groove 7. The X-axis linear module 8 drives the inoculation loop 23 after dipping the sample to approach the through groove 7 and stop above the edge of the culture dish 16 after the first pass. The Z-axis linear module 9 drives the tilted inoculation loop 23 downward to make the marking end 18 of the sample contact the inner surface of the culture dish 16. The X-axis linear module 8 continues to drive the inoculating ring 23 in the reverse direction to move, so that the marking end 18 marks a straight line in the culture dish, and then the X-axis linear module 8 drives the inoculating ring 23 in the forward direction to return to the starting point of the straight line. At this time, the Y-axis linear module 20 drives the suction cup 17 in the forward and reverse directions alternately to drive the culture dish to move back and forth at a fixed distance in the through groove 7. At the same time, the X-axis linear module 8 drives the inoculating ring 23 in the reverse direction to make the marking end 18 draw another straight line in the culture dish 16. The two actions are combined to complete the Feng-shaped marking of the culture dish.

[0075] The multi-zone demarcation line method includes steps a1, a2, and a3, and further includes:

[0076] a4. Multiple demarcation lines: After step a3 is completed, one demarcation line is realized, and then the first rotary motor 19 drives the turntable to drive the culture dish 16 to rotate a certain angle, and the above steps are repeated until multiple demarcation lines are completed.

[0077] After the streaking and inoculation is completed, the Z-axis linear module drives the operating mechanism 24 to grasp the sample tube 14 downward using the clamping piece 33, and at the same time the fourth rotary motor 4 is started to tighten the tube cover 35 through the reverse rotation action.

[0078] The third rotary motor 27 is started, and the classification guide plate 28 is rotated in the opposite direction to guide it to the recovery bin 30 for recovering and identifying samples. Then the clamping mechanism 15 above the sampling slot 5 is opened, and the sample tube 14 slides through the classification guide plate 28 and the slide 29 to the recovery bin 30 for identifying samples.

[0079] The inoculated culture dish is driven by the suction cup of the plate pushing device to enter the plate flipping mechanism 50 of the plate pushing and lid opening and buckling device through the driving device, and is flipped and buckled by the plate flipping mechanism and sent to the conveyor belt 10 of the automatic labeling device. The conveyor belt 10 starts to label the inoculated culture dish, and then the labeled culture dish is sent to the storage port of the processed culture dish bin by the plate pushing device. The lifting cylinder of the lifting mechanism drives the lifting plate 47 to lift the culture dish upward and rotate the one-way limit plate 49 to drag the lifted culture dish, and then the lifting plate 47 falls back and lifts the next labeled culture dish. The operation is repeated. After the limiter recognizes the culture dish, it indicates that the single storage bin is full of culture dishes. At this time, the limiter generates a signal to the fifth drive motor, which controls the fifth rotary motor to drive the whole to rotate a certain angle through the controller 25 so that another storage port of the processed culture dish bin is facing the labeled culture dish. It should be noted that the culture dish route of the invention is only explained here by sending the processed culture dish into the storage bin. In actual production, the labeled culture dishes can be sent to the equipment of the next process through other means such as conveyor belts, and other designs can also be made as needed.

[0080] Of course, the above description is not limited to the above examples. Technical features not described in the present invention can be achieved by or by adopting existing technologies, which will not be described here. The above embodiments and drawings are only used to illustrate the technical solutions of the present invention and are not limitations of the present invention. The present invention is described in detail with reference to the preferred implementation methods. Ordinary technicians in this field should understand that changes, modifications, additions or substitutions made by ordinary technicians in this technical field within the essential scope of the present invention do not depart from the purpose of the present invention and should also fall within the scope of protection of the claims of the present invention.

Claims

1. A microbial sample pretreatment system comprising a workbench, an untreated culture dish compartment, and a controller. The workbench is equipped with a sample shaking device, a sample tube clamping and lid opening and closing device, a sterilizing device, a streaking device, a culture dish pushing and lid opening and closing device, an automatic labeling device, and a culture dish storage device, each of which is controlled by the controller. The system is characterized by: A sampling slot and a through slot are provided on both sides of the sterilizer on the workbench, and a line connecting the center of the sampling slot and the center of the sterilizer is perpendicular to the length of the through slot and passes through the midpoint of the length of the through slot. A clamping mechanism for controlling the clamping of the sample tube is installed above the sampling slot; The scribing device includes an XZ biaxial linear module arranged above the workbench, wherein: a controllable rotatable inoculation loop is installed on the slider of the Z-axis linear module; a Y-axis linear module is installed below the workbench, directly below the through slot, and arranged along the length of the through slot; a suction cup that can move within the through slot is installed on the slider of the Y-axis linear module, and the suction cup is connected to a vacuum pump through an air pipe; The unprocessed culture dish bin includes a top plate and a bottom plate fixed by connecting columns. The top plate is concavely provided with a plurality of arc-shaped upper notches from the edge of the top plate to the center of the top plate. The bottom plate is formed with arc-shaped lower notches that are directly opposite to the arc-shaped upper notches. Two supporting plates are fixed to the bottom plate through a connecting plate below each arc-shaped lower notch. A slide gap for taking and placing culture dishes is left between the two supporting plates and the connecting plate. A side of the connecting plate connecting the arc-shaped lower notches and the supporting plates is set as an arc-shaped inclined surface. The bottom plate and the top plate form an open placement bin between the supporting plates, the arc-shaped upper notches, and the arc-shaped lower notches. The system also includes a classification and recovery device controlled by a controller, the classification and recovery device including a barcode recognition system and a data storage system, and a barcode scanner mounted on a workbench facing the sampling slot and electrically connected to the barcode recognition system; the workbench is provided with two open recovery bins below the sampling slot, and a classification guide plate is mounted above the two recovery bins and rotated by a third rotary motor; A second rotary motor with an output shaft arranged along the Y-axis direction is installed on the slider of the Z-axis linear module. The output shaft of the second rotary motor is connected to at least one inoculation ring through a mounting block. The middle part of the inoculation ring is fixed on the mounting block, and the two ends of the inoculation ring are respectively provided with a marking end; By setting the sampling slot center, the sterilization device center and the midpoint of the through slot length to be collinear, inoculation, sterilization and marking operations can be completed in one direction during operation, and can be completed only by linear motion of the XZ biaxial linear module.

2. The microbial sample pretreatment system according to claim 1, characterized in that: A first rotating motor is installed on the slider of the Y-axis linear module, and a suction cup is installed on the output shaft of the first rotating motor. A cavity is provided in the output shaft and a through hole communicating with the outside world is opened. One end of the air pipe enters the cavity through the through hole and is connected to the suction cup.

3. The microbial sample pretreatment system according to claim 1, characterized in that: The sample tube clamping and lid opening and closing device includes a motion mechanism and an operating mechanism. The motion mechanism includes an XYZ three-axis linear module electrically connected to a controller; the operating mechanism includes a fixed rod, one end of which is provided with at least two clamping pieces located on the same circle, the diameter of the circle is smaller than the diameter of the sample tube cover, the middle parts of the two clamping pieces are rotatably connected to one end of the fixed rod, and the upper parts of the two clamping pieces are respectively connected to the fixed rod through springs.

4. The microbial sample pretreatment system according to claim 3, characterized in that: The other end of the fixing rod is connected to a fourth rotary motor, and the fourth rotary motor is mounted on a slider of the Z-axis linear module.

5. The microbial sample pretreatment system according to claim 3, characterized in that: The sample tube clamping and lid opening and covering device also includes a photographing device installed on the Z-axis linear module slider and used to identify the color of the sample tube cover.

6. The microbial sample pretreatment system according to claim 1, characterized in that: The sample shaking device comprises a shaking table installed in a workbench. A sample tray with a handle is detachably connected to the shaking table. The sample tray is provided with a plurality of tube holes that match the sizes of the sample tubes.

7. The microbial sample pretreatment system according to claim 1, characterized in that: The culture dish storage device is detachably connected to the workbench, and includes a processed culture dish bin and a lifting mechanism. The processed culture dish bin includes an upper splint and a lower splint fixed by a connecting column. The upper splint is circumferentially concave from the edge to the center to form a plurality of arc-shaped upper notches. The lower splint is formed with arc-shaped lower notches that are opposite to the arc-shaped upper notches one by one. The lower splint and the upper splint form an open storage bin between the arc-shaped upper notches and the arc-shaped lower notches. The lower splint is hinged with a one-way limit plate that can only rotate upward at the edge of each arc-shaped lower notch. The upper splint is provided with a limit switch for detecting the position of the culture dish at the edge of each arc-shaped upper notch. The center of the lower splint is connected to a fifth rotating motor electrically connected to the limiter; the lifting mechanism is arranged between the outlet of the automatic labeling device and the processed culture dish bin, and the lifting mechanism includes a lifting plate matching the arc-shaped lower notch, and a lifting cylinder for driving the lifting plate to move up and down.

8. The microbial sample pretreatment system according to claim 1, characterized in that: It also includes a shell, a touch screen electrically connected to the controller is provided on the outside of the shell, and an emergency stop button, a reset button, a stop button, a start button and a switch button are provided on the display screen. An ultraviolet lamp, a lighting lamp and an air purifier are installed in the shell, and an exhaust fan is installed on one side of the shell.

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