A dense light detection device

Abstract

The present invention discloses a dense light detection device, including a box body, an inner dense light chamber, an outer dense light chamber, and a driving mechanism for driving a reaction tube to pass through an avoidance through hole and reciprocate between a liquid adding station in the outer dense light chamber and the inner dense light chamber, and a detection hole is provided on the side wall of the inner dense light chamber; a liquid adding mechanism and a luminous detection mechanism for photon counting are provided on the box body, and a dense light component for shielding the detection hole is provided in the inner dense light chamber. When the transport mechanism drives the reaction tube to be transported to the liquid adding station corresponding to the liquid adding mechanism in the inner dense light chamber, the dense light component removes the shielding of the detection hole, so that the luminous detection mechanism realizes the luminous value detection of the reaction tube through the detection hole. The dense light detection device disclosed by the present invention has fast and stable sample transportation, and the transportation process is in a dense light environment. In addition, the photon counter is also completely dense light in a non-working state to avoid the influence of external light and improve the detection accuracy.

Description

Technical Field

[0001] The invention relates to the technical field of medical instruments, in particular to a dense light detection device. Background Art

[0002] Immunoluminescence detection uses the principle of luminescence (including spontaneous luminescence, electroluminescence or chemiluminescence) of the substance being detected to detect and count the luminescence process and convert it into the concentration of the substance being detected to determine the test result. In the early stage of the detection process, the sample to be tested needs to be centrifuged, mixed, and incubated for reaction, and in the later stage of the detection process, the luminescent substance needs to be detected. Detection is usually carried out in a dense light environment.

[0003] In semi-automatic or fully automatic instruments, the insertion and removal of the test tubes and the addition of reagents such as the stimulating solution are all carried out in an open environment, which brings about problems such as insufficient instrument density and high background values, thus affecting the consistency and accuracy of the test results. In existing fully automatic immunoluminescence analyzers, in-situ detection structures are mostly used, that is, after the sample is incubated with a special reagent, a certain density structure and detection device are attached to a certain position on the annular disk or chain. Due to the limitations of the test tube density and the in-and-out gap, the density performance is reduced, the detection background value is high, and other defects, which reduce the sensitivity of the detection. Summary of the invention

[0004] The technical problem to be solved by the present invention is to overcome the defects of the prior art and provide a dense light detection device, in which the sample is transported quickly and stably, and the transportation process is in a dense light environment. In addition, the photon counter is also completely dense in the non-working state to avoid the influence of external light and improve the detection accuracy.

[0005] The technical solution adopted by the present invention is: to provide a dense light detection device, comprising a box body, wherein an inner dense light chamber and an outer dense light chamber are respectively arranged on both sides of the inner cavity of the box body, and an avoidance through hole is arranged on a side of the inner dense light chamber close to the outer dense light chamber;

[0006] The box body is provided with a liquid adding mechanism connected with the inner light chamber; the box body is also provided with a support frame for placing the reaction tube and a driving mechanism for driving the support frame to pass through the avoidance through hole and reciprocate between the outer light chamber and the inner light chamber;

[0007] A luminescence detection mechanism is also provided at a position on the outside of the box body corresponding to the inner dense light chamber, and a detection hole connected to the luminescence detection mechanism is provided on the side wall of the inner dense light chamber; a dense light component for shielding the detection hole is provided in the inner dense light chamber, and when the driving mechanism drives the reaction tube to be transported into the inner dense light chamber and is located below the liquid adding mechanism, the dense light component releases the shielding of the detection hole, so that the luminescence detection mechanism realizes the luminescence value detection of the reaction tube through the detection hole.

[0008] Compared with the prior art, the present invention has the following advantages:

[0009] 1) An operating box is divided into an outer dense light chamber and an inner dense light chamber. The entire transmission component is arranged in the outer dense light chamber. The PMT structure corresponds to the inner dense light chamber. The transmission mechanism arranged in the outer dense light chamber can realize the entry and exit of the detection sample more quickly, and at the same time realize the accurate addition of the excitation liquid in the dense light environment, thereby improving the detection efficiency.

[0010] 2) A dense light component corresponding to the detection hole is set in the inner dense light chamber, and the PMT detection hole is automatically covered or separated from the PMT detection hole according to the operating position of the transportation mechanism. Thanks to this, the PMT can be powered on for a long time, which improves the stability of the detection result and the detection speed; at the same time, the PMT will not be damaged by strong ambient light when not detecting.

[0011] 3) The detection structure of the novel detection device is simple, and no other complex dense light control structure is required, which improves the detection efficiency and reduces the cost and failure rate.

[0012] Furthermore, a dense light cover is provided on the support frame, and when the driving mechanism drives the support frame to move to the liquid adding station in the inner dense light chamber, the circumferential outer edge of the dense light cover cooperates with the inner wall of the avoidance through hole. In this structure, the extinction structure added at the connection between the inner dense light chamber and the outer dense light chamber more effectively blocks the light and ensures the dense light environment of the inner dense light chamber.

[0013] Furthermore, the driving assembly includes a driving motor and a transmission belt, the driving motor is connected to the transmission belt by transmission, and the transmission belt is arranged along the length direction of the box; the lower end of the support frame is connected to the transmission belt. The structure is simple, and the transmission is accurate and stable.

[0014] As an improvement, a guide rail is provided on the bottom plate of the inner cavity of the box body along its length direction, a slider is slidably arranged on the guide rail, and the lower end of the support frame is connected to the slider. The setting of this improved structure makes the operation of the support frame more stable, and it can smoothly pass through the avoidance through hole every time without interference and collision of the edge.

[0015] In a further improvement, a chopper is provided at the lower end of the support frame, and a detection optical coupler is provided in the outer dense light chamber, which is used to detect whether the transport mechanism transports the reaction tube to the set position in the inner dense light chamber. In this improved structure, the support frame movement stroke is determined by detecting the optical coupler and the chopper, which has a simple structure, sensitive response, and accurate position judgment; it ensures that every time the support frame moves to the inner dense light chamber and stops, the reaction tube on the support frame can be accurately located at the liquid adding position, ensuring that the subsequent liquid adding process is more accurate and faster, and improving the detection efficiency.

[0016] Preferably, the dense light assembly includes a dense light block, and a sliding groove that slides with the dense light block is provided on the inner side wall of the inner dense light chamber along its length direction; and an elastic member is provided between the dense light block and the side wall of the inner dense light chamber at one end away from the avoidance through hole, so that the dense light block always has a tendency to move toward the avoidance through hole.

[0017] In a further improvement, a limiting boss is provided at one end of the slide groove near the avoidance through hole. This improved mechanism is used to limit the movement stroke of the dense light block to prevent the dense light block from being separated from the inner dense light chamber under the elastic force of the elastic member.

[0018] In a further improvement, the support frame is further provided with a baffle plate parallel to the detection hole, the baffle plate is provided with a light-transmitting hole, and the aperture of the light-transmitting hole is smaller than the aperture of the detection hole. The setting of this improved structure can ensure the consistency of the detection area of ​​the light-emitting part during each light quantum detection, that is, each detection area is the area of ​​the light-transmitting hole.

[0019] In a further improvement, a reflector is provided on the side wall of the inner light chamber away from the detection hole, corresponding to the detection hole. The reflector is added in this improved structure, which can effectively increase the photon intensity of the reaction light in the reaction tube, and can improve the detection accuracy when the photon is relatively weak.

[0020] Further improved, the mating part of the light-tightening cover plate and the avoidance through hole is a circular stepped fitting structure. The stepped fitting design adopted in this improved structure more effectively realizes the sealing of light, and the circular outer contour is adopted so that the potential light leakage boundary is only an arc line, which is less likely to have light leakage at the corners compared with other polygons, and the light-tightening effect is better; thereby ensuring a sufficient light-tightening environment in the inner light-tightening chamber and avoiding the influence of ambient light on the detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic structural diagram of the dense light detection device of the present invention.

[0022] Figure 2 It is a schematic structural diagram of the dense light detection device of the present invention in a non-detection state after the box body is removed.

[0023] Figure 3 It is a structural schematic diagram of the detection state of the dense light detection device of the present invention after the box body is removed.

[0024] Figure 4 It is a longitudinal cross-sectional view of the dense light detection device of the present invention in a non-detection state.

[0025] Figure 5 It is a longitudinal cross-sectional view of the detection state of the dense light detection device of the present invention.

[0026] Figure 6It is a transverse cross-sectional view of the dense light detection device of the present invention in a non-detection state.

[0027] Figure 7 It is a transverse cross-sectional view of the detection state of the dense light detection device of the present invention.

[0028] As shown in the figure: 100-reaction tube, 01-inner light chamber, 02-outer light chamber, 03-liquid adding mechanism, 04-luminescence detection mechanism;

[0029] 1-box, 2-avoidance through hole, 3-detection hole, 4-support frame, 5-dense light cover, 5.1-step protrusion, 6-drive motor, 7-transmission belt, 8-guide rail, 9-slider, 10-chopper, 11-detection optical coupler, 12-dense smooth block, 13-slide, 14-elastic part, 15-limiting boss, 16-baffle, 16.1-light-transmitting hole, 17-reflector, 18-operation hole, 19-driving wheel, 20-sensor, 21-mounting block, 22-mounting hole, 23-fixing block. DETAILED DESCRIPTION

[0030] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0031] In the description of the present invention, it should be noted that the terms "inside, outside", "bottom plate", "outer side wall", "lower end", "below", "upper end" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they cannot be understood as limitations on the present invention.

[0032] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the term "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0033] like Figure 1 , 2As shown, the present invention provides a dense light detection device, which includes a rectangular box body 1, an inner dense light chamber 01 is provided in the box body 1, and is arranged at one end of the inner cavity of the box body 1 in the length direction; an outer dense light chamber 02 is arranged at the other end of the box body 1 in the length direction, and an avoidance through hole 2 is provided on the side of the inner dense light chamber 01 close to the outer dense light chamber 02; specifically, in this embodiment, a mounting block 21 is provided at one end of the inner cavity of the box body 1, and a receiving cavity for receiving a support frame 4 is provided in the mounting block 21, and the receiving cavity forms the inner dense light chamber 01, and the area between the outside of the mounting block and the inner side wall of the box body 1 in the box body 1 forms the outer dense light chamber 02, and the avoidance through hole 2 is provided on the side wall of the receiving cavity close to one end of the outer dense light chamber 02;

[0034] The box body 1 is also provided with a liquid adding mechanism 03, which is specifically provided at the upper end of the box body 1, and the liquid adding pipe of the liquid adding mechanism 03 extends into the inner cavity of the inner light chamber 01, and the position corresponding to the liquid adding mechanism 03 in the inner light chamber is the liquid adding station; when the reaction tube 100 runs to the liquid adding station, the liquid adding mechanism can add the corresponding excitation liquid of the luminescent reaction into the reaction tube 100;

[0035] The box body 1 is also provided with a support frame 4 for placing the reaction tube 100 and a driving mechanism for driving the reaction tube 100 to reciprocate between the liquid adding station in the outer light-tight chamber 02 and the inner light-tight chamber 01 through the avoidance through hole 2;

[0036] The outer side of the box body 1 is also provided with a light-emitting detection mechanism 04, which is arranged at a position corresponding to the inner light chamber 01 on a side wall in the width direction of the box body 1, and a detection hole 3 is arranged on the side wall of the inner light chamber 01, and the position of the detection hole 3 corresponds to the detection end of the light-emitting detection mechanism 04. The light-emitting detection mechanism 04 in this structure is a photon counter (PMT);

[0037] A dense light component for shielding the detection hole 3 is also provided in the inner dense light chamber 01. When the driving mechanism drives the reaction tube 100 to the liquid adding station in the inner dense light chamber 01, the dense light component releases the shielding of the detection hole 3, so that the luminescence detection mechanism 04 can detect the luminescence value of the reaction tube 100 through the detection hole 3. The above structure fully ensures that the luminescence detection mechanism 04 is in a dense light environment regardless of whether it is in the detection state or the non-use state, thereby ensuring the detection accuracy.

[0038] In this embodiment, preferably, the dense light assembly includes a dense smooth block 12, and a slide groove 13 that slides with the dense smooth block 12 is provided on the inner side wall of the inner dense light chamber 01 along its length direction; and an elastic member 14 is provided between the dense smooth block 12 and the side wall of the inner dense light chamber 01 at one end away from the avoidance through hole 3, so that the dense smooth block 12 always has a tendency to move toward the avoidance through hole 2, ensuring the shading of the detection hole 3, so that the light-emitting detection mechanism 04 is in a dense light environment, ensuring the precision and accuracy of the detection.

[0039] Improved, a limiting boss 15 is provided at one end of the slide groove 13 near the avoidance through hole 3, which is used to limit the movement stroke of the dense smooth block 12, so as to prevent the dense smooth block 12 from being separated from the inner dense light chamber 01 under the elastic force of the elastic member 14, thereby affecting the next detection. In this embodiment, the elastic member 14 is preferably a cylindrical spring, and a corresponding spring hole 12.1 is provided on the dense smooth block 12, such as Figure 4 As shown, one end of the cylindrical spring is fitted in the spring hole 12.1, and the other end is against the side wall of the inner dense light chamber 01 away from the avoidance through hole 2. In order to ensure that the dense light block 12 slides more stably, there are two cylindrical springs in this embodiment, and the two cylindrical springs are distributed vertically.

[0040] In this embodiment, Figure 2 As shown, the support frame 4 is provided with an insertion hole for placing the reaction tube 100, and the driving mechanism is connected to the support frame 4 in a transmission manner, and is used to drive the support frame 4 to reciprocate along the length direction of the box body 1. In addition, in this structure, a dense light cover plate 5 is provided on the support frame 4. When the driving mechanism drives the support frame 4 to move to the liquid adding station in the inner dense light chamber 01, the outer wall of the dense light cover plate 5 cooperates with the inner wall of the avoidance through hole 2, thereby ensuring an absolutely dense light detection environment in the inner dense light chamber 01 and improving the detection accuracy. Because in this structure, an operation hole 18 for taking and placing the reaction tube 100 is opened at the top of the outer dense light chamber 02, a small amount of external light will enter the outer dense light chamber 02, so a corresponding dense light cover plate 5 structure is added to this structure. When the reaction tube 100 enters the inner dense light chamber 01 for detection, the dense light cover plate 5 is tightly matched in the avoidance through hole 2, avoiding the influence of a small amount of external light in the outer dense light chamber 02 on the detection structure, thereby ensuring the detection accuracy.

[0041] In this structure, Figure 5 As shown, the mating part of the tight light cover plate 5 and the avoidance through hole 2 is a stepped fitting structure, which realizes the sealing of light, ensures the tight light environment in the inner tight light chamber, and avoids the influence of ambient light on the detection result. Specifically, an annular stepped fitting groove 2.1 is set on the inner wall of the avoidance through hole 2, and a step protrusion 5.1 corresponding to the fitting groove 2.1 is correspondingly provided on the side of the tight light cover plate 5 near the inner tight light chamber 01. In addition, the stepped fitting structure in this structure is a circular structure, that is, the step protrusion 5.1 and the fitting groove 2.1 are both circular structures. After such a setting, the potential light leakage boundary at the mating edge position is only an arc line, which is less likely to have light leakage at the corners compared with other polygons, and the tight light effect is better.

[0042] More specifically, the driving mechanism in this embodiment includes a driving motor 6 and a conveyor belt 7, the driving motor 6 is connected to the conveyor belt 7 by transmission, and the conveyor belt 7 is arranged along the length direction of the box body 1; the lower end of the support frame 4 is connected to the driving belt 7. Preferably, the driving motor 6 is a stepping motor, which has more stable driving and accurate step distance.

[0043] like Figure 2 , 3 As shown in Figure 6, in this embodiment, the driving motor 6 is installed on the lower bottom surface of the box body 1 near the outer light chamber 02, and the output shaft of the driving motor 6 is connected to a driving wheel 19, which is arranged on the bottom surface of the outer light chamber 02. A driven wheel (not shown in the figure) is arranged at the other end of the inner cavity of the box body 1. The two ends of the transmission belt 7 are respectively matched with the outside of the driving wheel 19 and the driven wheel, so that the transmission belt 7 is driven by the driving motor 6 to run, thereby realizing the horizontal movement of the support frame 4. Figure 2 As shown, a guide rail 8 is provided on the bottom plate of the inner cavity of the box body 1 along its length direction, and a slider 9 is slidably provided on the guide rail 8. The lower end of the support frame 4 is connected to the slider 9, thereby further ensuring the linearity of the horizontal movement of the support frame 4, and no position deviation will occur during the movement process, ensuring that the support frame 4 can smoothly pass through the avoidance through hole 2 every time to transport the reaction tube 100 to the liquid adding station.

[0044] In addition, such as Figure 2 , 4 As shown, a chopper 10 is also provided at the lower end of the support frame 4, and a detection optical coupler 11 is provided at position 02 in the outer dense light chamber and below the inner dense light chamber 01, which is used to detect whether the transportation mechanism accurately transports the reaction tube 100 to the position corresponding to the liquid adding station in the inner dense light chamber 01.

[0045] The support frame 4 is also provided with a baffle 16 parallel to the detection hole 3 . The baffle 16 is provided with a light-transmitting hole 16 . 1 , and the aperture of the light-transmitting hole 16 . 1 is smaller than the aperture of the detection hole 3 .

[0046] In this embodiment, Figure 6 As shown, a reflector 17 is provided on the side wall of the inner light chamber 01 at one end away from the detection hole 03, corresponding to the detection hole 3, to increase the photon intensity after the reaction tube 100 reacts with light, and improve the detection accuracy. Specifically, in this embodiment, a mounting hole 22 is provided on the side wall of the accommodating cavity on the side opposite to the detection hole 3, and a corresponding fixing block 23 is inserted into the mounting hole 22, and a reflector 17 is provided on the end face of the fixing block 23 near the accommodating cavity, so that the disassembly and assembly of the reflector 17 is convenient for the design.

[0047] In the above structure, in order to ensure the automation of the detection process, a general controller is also provided in the detection device, and a corresponding sensor 20 is provided on the support frame 4. The sensor 20 is connected to the controller signal, and the drive motor 6, the detection optical coupler 11, the photon counter and the liquid adding mechanism 03 are all connected to the controller signal to improve the degree of automation.

[0048] Working principle:

[0049] First, the reaction tube 100 containing the sample is placed into the insertion hole on the support frame 4. At this time, the sensor 20 senses the presence of the reaction tube 100, and then transmits the signal to the controller. The controller controls the drive motor 6 to run, driving the support frame 4 to move toward the inner dense light chamber 01. When the support frame 4 enters the inner dense light chamber 01, the end of the support frame 4 pushes the dense sliding block 12 to slide in the direction away from the avoidance through hole 2 until the detection optical coupler 11 detects the chopping piece 10, and the drive motor 6 stops. At this time, the support frame 4 just moves to the liquid adding station, and the controller controls the liquid adding mechanism to add liquid to the reaction tube 100, and at the liquid adding station, the detection hole 3 is completely exposed. After waiting for the set time, the PMT is turned on, and the reaction tube is detected through the detection hole 3. 100 is used to detect the number of photons after the luminescent reaction; after the detection is completed, the controller controls the driving motor 6 to reset and drive the support frame 4 to reset to the outer dense light chamber 02. In the process of the support frame 4 moving away from the inner dense light chamber 01, since the dense smooth block 12 loses the thrust of the support frame 4, under the elastic force of the elastic member 14, the dense smooth block 12 resets and slides toward the position of the avoidance through hole 2 until the outer end surface of the dense smooth block 12 abuts against the limiting boss 15. At this time, the dense smooth block 12 completely blocks the detection hole 3 again to ensure that the PMT is not exposed to external light and is in a dense light environment; after the support frame 4 is reset, the reaction tube 100 is taken out, and then the next new reaction tube is put in to carry out the next cycle of detection, and this cycle is repeated to achieve rapid detection of samples.

[0050] The above is an explanation of the preferred embodiments of the present invention, but it cannot be understood as a limitation of the claims. The present invention is not limited to the above embodiments, and its specific structure is allowed to be changed. All changes made within the scope of protection of the independent claims of the present invention are within the scope of protection of the present invention.

Claims

1. A dense light detection device, characterized in that: The invention comprises a box body (1), wherein an inner light chamber (01) and an outer light chamber (02) are respectively provided on both sides of the inner cavity of the box body (1), and a side of the inner light chamber (01) close to the outer light chamber (02) is provided with an avoidance through hole (2); the box body (1) is provided with a liquid adding mechanism (03) connected to the inner light chamber (01); the box body (1) is also provided with a support frame (4) for placing a reaction tube (100) and a driving mechanism for driving the support frame (4) to pass through the avoidance through hole (2) and reciprocate between the outer light chamber (02) and the inner light chamber (01); A luminescence detection mechanism (04) is also provided at a position on the outside of the box (1) corresponding to the inner dense light chamber (01), and a detection hole (3) in communication with the luminescence detection mechanism (04) is provided on the side wall of the inner dense light chamber (01); a dense light component for shielding the detection hole (3) is provided in the inner dense light chamber (01); when the driving mechanism drives the reaction tube (100) to be transported into the inner dense light chamber (01) and is located below the liquid adding mechanism (03), the dense light component releases the shielding of the detection hole (3), so that the luminescence detection mechanism (04) detects the luminescence value of the reaction tube (100) through the detection hole (3); The support frame (4) is provided with a dense light cover plate (5). When the driving mechanism drives the support frame (4) to move into the inner dense light chamber (01) and is located below the liquid adding mechanism (03), the circumferential outer edge of the dense light cover plate (5) cooperates with the inner wall of the avoidance through hole (2); the dense light assembly includes a dense light block (12), and a sliding groove (13) that is slidably matched with the dense light block (12) is provided on the inner side wall of the inner dense light chamber (01) along its length direction; and the dense light block (12) and the inner dense light chamber are connected to each other. (01) An elastic member (14) is provided between the side walls at one end away from the avoidance through hole (2), so that the dense light block (12) always has a tendency to move toward the avoidance through hole (2); a limiting boss (15) is provided at one end of the slide groove (13) close to the avoidance through hole (2); a reflector (17) is provided on the side wall at one end of the inner dense light chamber (01) away from the detection hole (3) corresponding to the detection hole (3); the matching portion between the dense light cover plate (5) and the avoidance through hole (2) is a circular stepped assembly structure.

2. The dense light detection device according to claim 1, characterized in that: The driving mechanism comprises a driving motor (6) and a conveying belt (7), wherein the driving motor (6) is connected to the conveying belt (7) in a transmission manner, and the conveying belt (7) is arranged along the length direction of the box body (1); the lower end of the supporting frame (4) is connected to the conveying belt (7).

3. The dense light detection device according to claim 1, characterized in that: A guide rail (8) is provided on the bottom plate of the inner cavity of the box body (1) and extends along its length direction. A slider (9) is slidably provided on the guide rail (8). The lower end of the support frame (4) is connected to the slider (9).

4. The dense light detection device according to claim 3, characterized in that: A chopper (10) is also provided at the lower end of the support frame (4), and a detection optical coupler (11) is provided in the outer light chamber (02) for detecting whether the transport mechanism has transported the reaction tube (100) to a set position in the inner light chamber (01).

5. The dense light detection device according to claim 2, characterized in that: The support frame (4) is also provided with a baffle (16) parallel to the detection hole (3); the baffle (16) is provided with a light-transmitting hole (16.1); and the aperture of the light-transmitting hole (16.1) is smaller than the aperture of the detection hole (3).

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