A biological cell lysis preparation workstation

By designing a biological cell lysis preparation work station, using a combination of mechanized batch configuration and a rocking device, the problems of low manual configuration efficiency and culture fluid stratification are solved, and an efficient and safe biological cell lysis preparation process is achieved.

CN112812950BActive Publication Date: 2025-04-04SUZHOU BAOFANGU IND TECH CO LTD
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Patent Information

Application Number
CN202110096151.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2021-01-25
Publication Date
2025-04-04
Estimated Expiration
2041-01-25

AI Technical Summary

Technical Problem

In the existing biological cell lysis technology, artificially configured culture medium has low efficiency and high cost, and it is easy to cause culture medium to stratify, affecting the experimental results.

Method used

A biological cell lysis preparation workstation is designed, using mechanized batch configuration technology, and the feeding and unloading device is automatically supplied through the loading and unloading device. A horizontal rocking device and a vertical rocking device are added to the mounting plate, and alternately rotate to mix the culture liquid to ensure that the liquid injection operation is carried out in the confined space.

Benefits of technology

It improves the processing efficiency of biological cell lysis preparation, reduces labor costs, ensures uniform mixing of culture medium, reduces experimental errors, and provides a safe and efficient experimental environment.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention discloses a biological cell lysis preparation workstation, comprising a box assembly, a loading and unloading device, a horizontal shaking device, a vertical shaking device and a power transmission device. The loading and unloading device is tightly connected to the box assembly. The box assembly comprises a mounting plate. The horizontal shaking device is installed on one side of the upper end of the mounting plate. The vertical shaking device is installed on the side of the upper end of the mounting plate away from the horizontal shaking device. The power transmission device is tightly connected to the mounting plate. The bottom end of a liquid removal manipulator is tightly connected to the mounting plate. A plurality of liquid injection manipulators are arranged on the mounting plate. The liquid injection manipulators are tightly connected to the mounting plate. A plurality of screw-on manipulators are arranged on one side of a rotating disk along a circumferential direction. The bottom end of the screw-on manipulator is tightly connected to the rotating disk.
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Description

Technical Field

[0001] The invention relates to the technical field of biological cell lysis, in particular to a biological cell lysis preparation workstation. Background Art

[0002] At present, most biological cell lysis methods on the market use manual nutrient solution preparation, especially in laboratory preparation. However, when large-scale production is required, a large amount of manpower is often required, and the work efficiency is low. Due to differences in personal levels, the preparation of the culture solution may deviate, affecting the final experimental results. In addition, in order to simulate the special living environment of fungi, some special substances are often added to the culture solution. These special substances are directly exposed to the air and may cause harm to human health. If they are isolated by wearing protective clothing, it will not be conducive to experimental operations. A separate closed laboratory is also required to prevent leakage, which increases the cost of the experiment. Especially when large-scale preparation is carried out, a large number of people are required to participate in order to improve efficiency, and the space is small, which is not conducive to operation. In addition, due to the large difference in the density of some culture solutions, simple filling will cause stratification of the culture solution, which is not conducive to the later lysis generation. Artificial mixing in large-scale experiments increases the difficulty of preparation. Summary of the invention

[0003] The object of the present invention is to provide a biological cell lysis preparation workstation to solve the problems raised in the above background technology.

[0004] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0005] A biological cell lysis preparation workstation includes a box assembly, a loading and unloading device, a horizontal shaking device, a vertical shaking device and a power transmission device. The loading and unloading device is tightly connected to the box assembly. The box assembly includes a mounting plate. The horizontal shaking device is installed on one side of the upper end of the mounting plate. The vertical shaking device is installed on the side of the upper end of the mounting plate away from the horizontal shaking device. The power transmission device is tightly connected to the mounting plate.

[0006] The present invention performs mechanized batch configuration of target bottles for biological cell lysis to replace manual configuration, thereby ensuring processing efficiency and reducing labor costs. The target bottles are automatically fed by loading and unloading devices, and the mounting plate is the main mounting substrate. Since the device controls the processing process through a controller, the precision is high, the configuration process weakens the vibration effect, and the amount of culture solution injected at a single time is relatively small. The culture solution injected into the target bottle in batches is easy to be stratified due to its high density. In order to make the culture solution in the target bottle mixed evenly, a horizontal shaking device and a vertical shaking device are added to the upper surface of the mounting plate. The horizontal shaking device picks up the target bottle through a chuck and rotates in a horizontal plane to mix the culture solution injected in batches. In order to ensure that the target bottle does not fall off the chuck, the controller controls the target bottle to rotate at a lower speed. The lower speed will cause insufficient mixing of the culture solution. Therefore, after mixing, the target bottle is transferred to the next station to inject the culture solution again, and then the target bottle is rotated and shaken in a vertical plane through the vertical shaking device. The culture solution is further mixed by rotating alternately in the horizontal plane and the vertical plane. The power transmission device and the mounting plate are tightly connected to ensure smooth torque transmission.

[0007] Furthermore, the box assembly also includes an upper frame, a lower frame and an alarm light. One side of the upper frame is sealed and connected to the mounting plate, one side of the lower frame is tightly connected to the mounting plate away from one side of the upper frame, and the alarm light is installed at the upper end of the upper frame.

[0008] In order to provide an ideal experimental space for biological cell lysis preparation, a closed space is formed between the upper rack and the mounting plate, which is the main working space. The target bottle remains sealed when entering the tooling device, and is only opened for liquid injection inside the device to prevent foreign matter from falling into the target bottle and affecting the experimental results. The mounting plate is the main load-bearing plate. In order to prevent positioning deviation caused by collision and vibration, the lower rack and the mounting plate are tightly connected. In order to facilitate the detection of the working condition of the special machine, an alarm light is set on the top. When the equipment fails or the operation is incorrect, the alarm light flashes.

[0009] Furthermore, the loading and unloading device includes a loading assembly, a transmission assembly and a wire protection chain. A plurality of safety baffles are provided at the upper end of the loading assembly. The loading assembly is fastened to the mounting plate. The transmission assembly is fastened to one side of the wire protection chain. The loading assembly includes a loading cylinder, a loading stacker, a transmission block and a stacking bracket. One side of the loading cylinder is fastened to the stacking bracket. The output end of the loading cylinder is fastened to the transmission block. Loading guide rails are provided on both sides of the bottom end of the loading stacker. A slider is provided at the top of the stacking bracket. The slider of the stacking bracket is slidably connected to the loading guide rail. The middle of the bottom end of the loading stacker is fastened to the transmission block. The transmission assembly is fastened to the mounting plate. The transmission assembly includes a sliding cylinder, a lead screw, a nut, a bearing seat, a transmission bracket, a transmission chuck, a chuck fixing plate, a transmission cylinder and a transmission slider. One side of the sliding cylinder is fastened to the transmission support The frame is tightly connected, the output end of the sliding cylinder and one side of the screw are tightly connected, both ends of the screw are movably connected to the bearing seat, one end of the transmission bracket is provided with an installation cavity, the bearing seat and the inner wall of the installation cavity of the transmission bracket are tightly connected, the nut and the screw are threaded, the outer cylindrical surface of the nut and one side of the transmission slider are tightly connected, one side of the transmission bracket and the wire protection chain are tightly connected, the bottom end of the transmission bracket and the mounting plate are tightly connected, one side of the transmission bracket is provided with a transmission guide rail, the transmission slider and the transmission guide rail are slidably connected, one side of the transmission slider is provided with a guide rail, the transmission slider guide rail and the transmission guide rail are perpendicular, one side of the chuck fixing plate is provided with a slider, the chuck fixing plate slider and the transmission slider guide rail are slidably connected, one side of the transmission cylinder and the transmission slider are tightly connected, the output end of the transmission cylinder is tightly connected to the chuck fixing plate, and the chuck fixing plate and the transmission chuck are tightly connected.

[0010] Safety baffles are set around the loading assembly to prevent the target bottle from tilting during transfer and installation. The loading assembly and the mounting plate are tightly connected to reduce the impact of the working vibration of the loading cylinder on loading. The power line and the control line are fixed uniformly by the wire protection chain. In order to prevent the line from interfering with the movement, the wire protection chain is imprisoned at one end of the transmission bracket. The wire protection chain can freely expand and contract with the movement of the nut. In order to improve the efficiency of culture fluid infusion, several target bottles are neatly placed on the loading stacking and automatically picked up by the transmission chuck. One side of the loading cylinder is tightly connected to the stacking bracket, and the loading cylinder is installed and positioned. The output end of the loading cylinder is tightly connected to the transmission block. The reciprocating motion of the transmission block is controlled by the movement of the cylinder. The middle of the bottom end of the loading stacking is tightly connected to the transmission block. In order to accurately control the movement of the loading stacking, guide rails and sliders are added. The loading stacking movement is guided by the sliding connection. As the target bottles close to the side of the transmission chuck are filled and sealed, the loading stacking is controlled by the cylinder to move to the side of the transmission chuck until all the target bottles are filled and sealed. After the standard bottle is filled, the alarm light comes on and it is replaced. Since several target bottles are arranged vertically and horizontally on the loading stack, the transmission chuck is controlled by the sliding cylinder to move in the horizontal direction. In order to facilitate the transmission, the sliding cylinder is installed on one side of the transmission bracket, and the output end and the lead screw are tightly connected. The positioning control is more precise through threaded engagement, and the number of transmission chuck adjustments is reduced. The two bearing seats are installed in the installation cavity of the transmission bracket. The built-in bearing and the two ends of the lead screw are movably connected so that the lead screw can rotate along the axis and control the transmission slider tightly connected to the nut to move in the horizontal plane. When it moves to the top of the target bottle, the chuck fixing plate is controlled by the transmission cylinder to move in the vertical direction until the chuck touches the target bottle mouth and clamps the target bottle. Then the transmission cylinder controls the chuck to move up, and the sliding cylinder controls the target bottle to move horizontally to the next station. Target bottles of different heights can be picked up by vertical movement, which is more practical. Through the fastening connection between the components, the control accuracy of the control machine is higher, and the processing efficiency is further improved.

[0011] Furthermore, the horizontal shaking device includes a horizontal shaking bracket, a horizontal shaking beam, a horizontal shaking slider, a horizontal shaking cylinder, a horizontal turntable, a first motor, a first guide column and a first slider. The top of the horizontal shaking bracket is tightly connected to one side of the horizontal shaking beam, the bottom of the horizontal shaking bracket is tightly connected to the mounting plate, one side of the horizontal shaking beam is provided with a horizontal shaking guide rail, the horizontal shaking slider is slidably connected to the horizontal shaking guide rail, the top of the horizontal shaking slider is tightly connected to the horizontal shaking cylinder, and one side of the horizontal shaking slider is provided with a guide rail perpendicular to the horizontal shaking guide rail. A vertical guide rail of the rail, a slider is provided on one side of the first motor, the first motor slider is slidably connected to the vertical guide rail of the horizontal liquid shaking slider, the output end of the horizontal liquid shaking cylinder is tightly connected to the first motor slider, the output end of the first motor is tightly connected to the horizontal turntable, a plurality of horizontal liquid shaking chucks are provided on the bottom surface of the horizontal turntable, a guide column mounting groove is provided on one side of the horizontal liquid shaking beam, the first guide column and the end face of the guide column mounting groove of the horizontal liquid shaking beam are tightly connected, the first slider is slidably connected to the first guide column, and the first slider is tightly connected to the horizontal liquid shaking slider on the side away from the first guide column.

[0012] The horizontal shaking device is installed on one side of the mounting plate. In order to facilitate the picking up of the target bottle, two sets of guide rail slider mechanisms are arranged on the horizontal shaking device to guide the movement. In order to prevent the horizontal shaking from colliding with other parts, the target bottle is moved horizontally away from the screwing manipulator through the horizontally arranged guide rail slider mechanism, and the target bottles of different heights are picked up through the vertically arranged guide rail slider mechanism. Since the linear displacement of the horizontal shaking chuck is controlled by the cylinder, the influence of vibration on the displacement is reduced by tightening the connection. In order to improve the integration performance of the horizontal shaking device, a guide rail is arranged on the horizontal shaking slider, and a slider is arranged on the side of the first motor. The output end of the horizontal shaking cylinder It is tightly connected to the first motor slider to accurately control the up and down movement of the first motor. The output end of the first motor is tightly connected to the horizontal turntable. The horizontal turntable is controlled to rotate along the axis by the rotation of the first motor. The culture solution in the target bottle is gradually mixed by rotation. A number of horizontal liquid shaking chucks are provided on the lower surface of the horizontal turntable to control the rotation of multiple target bottles to improve work efficiency. The horizontal liquid shaking chucks are controlled by a small cylinder to clamp the bottle mouth of the target bottle. Each horizontal liquid shaking chuck is equipped with a small cylinder to control the horizontal liquid shaking chuck individually. A guide column slider mechanism is added to the horizontal liquid shaking beam to cooperate with the guide rail slider mechanism to perform bidirectional positioning on the horizontal movement, so that the displacement accuracy is higher.

[0013] Furthermore, the vertical shaking liquid device includes a vertical shaking liquid bracket, a vertical shaking liquid beam, a vertical shaking liquid slider, a vertical shaking liquid cylinder, a vertical turntable, a second motor, a second guide column and a second slider. The top of the vertical shaking liquid bracket is tightly connected to one side of the vertical shaking liquid beam, the bottom of the vertical shaking liquid bracket is tightly connected to the mounting plate, one side of the vertical shaking liquid beam is provided with a vertical shaking liquid guide rail, the vertical shaking liquid slider is slidably connected to the vertical shaking liquid guide rail, one side of the vertical shaking liquid slider is tightly connected to the vertical shaking liquid cylinder, and one side of the vertical shaking liquid slider is provided with a guide rail perpendicular to the vertical shaking liquid guide rail. A vertical guide rail of the rail, a slider is provided on one side of the second motor, the second motor slider and the vertical guide rail of the vertical liquid shaking slider are slidably connected, the output end of the vertical liquid shaking cylinder is tightly connected to the second motor slider, the second motor output end and the vertical turntable are tightly connected, a plurality of vertical liquid shaking chucks are provided on the bottom surface of the vertical turntable, a guide column mounting groove is provided on one side of the vertical liquid shaking beam, the second guide column and the end face of the guide column mounting groove of the vertical liquid shaking beam are tightly connected, the second slider is slidably connected to the second guide column, and the second slider is tightly connected to the vertical liquid shaking slider on the side away from the second guide column.

[0014] After the horizontal shaking, the target bottle is sent to the screwing robot, which unscrews the target bottle cap, and as the rotating disk rotates, the culture solution is injected into the target bottle again by the injection robot. After the second injection, the bottle cap is tightened by the screwing robot. As the components of the culture solution increase, the horizontal shaking can no longer mix the culture solution more evenly, and it is further mixed by the vertical shaking device. After two mixings, the culture solution is no longer stratified, and the vertical shaking bracket, the vertical shaking beam and the mounting plate are connected more tightly by fastening, thereby weakening the positioning error caused by external collision. A vertical shaking guide rail is provided on one side of the vertical beam, which is slidably connected to the vertical shaking slider to guide the horizontal displacement, and is bidirectionally positioned by the guide column slider mechanism to improve the positioning accuracy. One side of the vertical shaking cylinder is tightly connected to the vertical shaking slider, so that the vertical displacement mechanism moves horizontally with the movement of the slider, and then the vertical shaking guide rail provided on the vertical shaking slider is used to make the first The second motor moves in the vertical direction, and as the second motor moves downward, several vertical shaking liquid chucks arranged on the vertical turntable touch the mouth of the target bottle, and the vertical shaking liquid chucks are controlled by a small cylinder to clamp the mouth of the target bottle. In order to prevent the target bottle from colliding with other devices when the vertical turntable rotates, the chuck height is increased by a vertically arranged guide slider mechanism, and the vertical turntable is away from the screw-on manipulator by a horizontally arranged guide slider mechanism. The second motor drives the vertical turntable to rotate along the axis, and the target bottle rotates accordingly. The culture solution in the target bottle is flipped and mixed in the vertical plane, further improving the mixing degree of the culture solution in the target bottle. There are two vertical shaking liquid devices, which pick up the target bottles alternately, shortening the waiting time for the vertical shaking liquid chuck to clamp the target bottle. Several vertical shaking liquid chucks are arranged under the vertical turntable, which can continuously pick up the target bottle as the turntable rotates, and then driven by the second motor, multiple target bottles are shaken at the same time, greatly improving the efficiency of shaking liquid.

[0015] Furthermore, the power transmission device includes a driving motor, a reducer, a flange turntable, a rotating disk, a reducer bracket and an auxiliary rotating assembly. One side of the driving motor is rotatably connected to the reducer, the reducer and the reducer bracket are tightly connected, the reducer bracket extends upward and is provided with a support column, the reducer bracket support column and the mounting plate are tightly connected, the reducer and the flange turntable are tightly connected, the bottom end of the flange turntable and the rotating disk are tightly connected, and a plurality of auxiliary rotating assemblies are provided on the lower surface of the rotating disk, and the auxiliary rotating assembly includes a rotating wheel and a rotating wheel bracket, and the rotating wheel and the rotating wheel bracket are movably connected.

[0016] The present invention uses the driving motor as the main power source of the power transmission device. In order to improve the stability between the various components, the driving motor is directly connected to the rotating disk through a tightening connection to reduce the impact of vibration on torque transmission. Because the driving motor rotates too fast, it will cause unstable force transmission and inconvenient rotation. Therefore, the output speed is reduced by a reducer, and the reduced torque is transmitted to the rotating disk through the flange turntable. Since the contact area between the flange turntable and the rotating disk is relatively large, the torque output is smoother. In order to reduce the radial force on the output shaft of the reducer when the rotating disk rotates, a number of auxiliary rotating components are arranged in a circumferential direction at the bottom of the rotating disk, and are installed on the rotating disk through a turntable bracket. As the rotating disk rotates, the turntable rolls on the surface of the mounting plate to support the rotating disk, thereby further improving the stability of the rotation of the rotating disk.

[0017] Furthermore, the preparation workstation also includes a liquid removal robot, the bottom end of which is tightly connected to the mounting plate, a plurality of liquid injection robots are arranged on the mounting plate, the liquid injection robots are tightly connected to the mounting plate, and a plurality of screwing robots are arranged along the circumferential direction on one side of the rotating disk, the bottom end of the screwing robot is tightly connected to the rotating disk.

[0018] After the target bottle is sent to the screw-on robot for uncapping, the target bottle is clamped out by the liquid removal robot and inverted to remove the residual liquid in the target bottle after cleaning, and the remaining liquid is collected by the receiving plate at the bottom of the liquid removal robot. Multi-component culture medium can be configured by setting multiple liquid injection robots. Each robot controls the cylinder to work through a controller, and the vibration is relatively large. Through fastening connection, the work is smoother. In order to improve work efficiency, a plurality of screw-on robots are arranged on the rotating disk, so that each workstation corresponds to at least one screw-on robot, reducing the transmission path between adjacent workstations and improving the injection efficiency. In addition to the screw-on operation, the screw-on robot also has the function of spraying codes on the target bottle, which is convenient for distinguishing the target bottles, especially when configuring large quantities of culture medium, greatly improving the classification efficiency.

[0019] Compared with the prior art, the beneficial effects achieved by the present invention are as follows: the present invention ensures processing efficiency and reduces labor costs by mechanized batch configuration of target bottles for biological cell lysis instead of manual configuration, and automatically feeds the target bottles through loading and unloading devices; a horizontal shaking device and a vertical shaking device are added to the upper surface of the mounting plate, and the culture solution is mixed evenly by alternately rotating the horizontal and vertical surfaces; a closed space is formed between the upper frame and the mounting plate, and the target bottles remain sealed when entering the tooling device, and only when they are opened and filled with liquid in the device can foreign matter from outside be prevented from falling into the target bottles and affecting the experimental results; two horizontal shaking devices are arranged on the horizontal shaking device. A guide rail slider mechanism is formed, and the target bottle is moved horizontally away from the screw-on robot through the horizontally arranged guide rail slider mechanism, so that other parts will not be collided during horizontal shaking of the liquid, and the target bottles at different heights are picked up through the vertically arranged guide rail slider mechanism; by arranging a number of auxiliary rotating components along the circumferential direction at the bottom end of the rotating disk, as the rotating disk rotates, the rotating wheel rolls on the surface of the mounting plate, which supports the rotating disk and further improves the stability of the rotation of the rotating disk; in addition to the capping operation, the screw-on robot also has the function of spraying codes on the target bottles, which is convenient for distinguishing the target bottles, especially when configuring large quantities of culture fluid, greatly improving the classification efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0021] Figure 1 It is a schematic diagram of the operating structure of the present invention;

[0022] Figure 2 It is a schematic diagram of the structure of the box assembly of the present invention;

[0023] Figure 3 It is a structural schematic diagram of the loading and unloading device of the present invention;

[0024] Figure 4 yes Figure 3 The left view of the view;

[0025] Figure 5 It is a schematic diagram of the structure of a horizontal liquid shaking device;

[0026] Figure 6 yes Figure 5 A top view of the view;

[0027] Figure 7 It is a schematic diagram of the structure of a vertical shaking liquid device;

[0028] Figure 8 yes Figure 7 The left view of the view;

[0029] Fig. 9 yes Figure 7 A top view of the view;

[0030] Fig.10 It is a schematic structural diagram of the power transmission device of the present invention;

[0031] Fig.11 This is the overall installation structure diagram of the present invention

[0032] In the figure: 1-box assembly, 11-upper frame, 12-lower frame, 13-mounting plate, 14-alarm light, 2-loading and unloading device, 21-loading assembly, 211-loading cylinder, 212-loading palletizing, 213-transmission block, 214-palletizing bracket, 215-loading guide rail, 22-transmission assembly, 221-sliding cylinder, 222-screw, 223-nut, 224-bearing seat, 225-transmission bracket, 2251-transmission guide rail, 226-transmission chuck, 227-chuck fixing plate, 228-transmission cylinder, 229-transmission slide, 23-safety baffle, 24-wire chain, 3-liquid removal manipulator, 4-horizontal shaking liquid device, 41-horizontal shaking liquid bracket, 42-horizontal shaking liquid beam, 421-horizontal shaking liquid guide rail, 43-horizontal liquid shaking slider, 44-horizontal liquid shaking cylinder, 45-horizontal turntable, 46-first motor, 47-horizontal liquid shaking chuck, 48-first guide column, 49-first slider, 5-vertical liquid shaking device, 51-vertical liquid shaking bracket, 52-vertical liquid shaking beam, 521-vertical liquid shaking guide rail, 53-vertical liquid shaking slider, 54-vertical liquid shaking cylinder, 55-vertical turntable, 56-second motor, 57-vertical liquid shaking chuck, 58-second guide column, 59-second slider, 6-screwing manipulator, 7-power transmission device, 71-drive motor, 72-reducer, 73-flange turntable, 74-rotating disk, 75-reducer bracket, 76-auxiliary rotating assembly, 761-rotating wheel, 762-rotating wheel bracket, 8-liquid injection manipulator. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] The present invention provides a technical solution:

[0035] like Figure 1 , 11As shown, a biological cell lysis preparation workstation includes a box assembly 1, a loading and unloading device 2, a horizontal shaking device 4, a vertical shaking device 5 and a power transmission device 7. The loading and unloading device 2 is tightly connected to the box assembly 1, and the box assembly 1 includes a mounting plate 13. The horizontal shaking device 4 is installed on one side of the upper end of the mounting plate 13, and the vertical shaking device 5 is installed on the side of the upper end of the mounting plate 13 away from the horizontal shaking device 4. The power transmission device 7 is tightly connected to the mounting plate 13.

[0036] The present invention performs mechanized batch configuration of target bottles for biological cell lysis preparation to replace manual configuration, thereby ensuring processing efficiency and reducing labor costs. The target bottles are automatically fed by the loading and unloading device 2, and the mounting plate 13 is the main mounting substrate. Since the device controls the processing process through a controller, the accuracy is high, the configuration process weakens the vibration effect, and the amount of culture solution injected at a time is relatively small. The culture solution injected into the target bottle in batches is easy to stratify due to its high density. In order to make the culture solution in the target bottle evenly mixed, a horizontal shaking device 4 and a The vertical shaking device 5 and the horizontal shaking device 4 pick up the target bottle through the chuck and rotate it in the horizontal plane to mix the culture solution injected in batches. In order to ensure that the target bottle does not fall off the chuck, the controller controls the target bottle to rotate at a lower speed. The lower speed will cause insufficient mixing of the culture solution. Therefore, after mixing, it will be transferred to the next station to inject the culture solution again. Then, the target bottle is rotated and shaken in the vertical plane by the vertical shaking device 5. The culture solution is further mixed by alternating rotation between the horizontal and vertical planes. The power transmission device 7 and the mounting plate 13 are tightly connected to ensure smooth torque transmission.

[0037] like Figure 2 As shown, the box assembly 1 also includes an upper frame 11, a lower frame 12 and an alarm light 14. One side of the upper frame 11 is sealed and connected to the mounting plate 13, one side of the lower frame 12 is tightly connected to the mounting plate 13 away from the side of the upper frame 11, and the alarm light 14 is installed on the upper end of the upper frame 11.

[0038] In order to provide an ideal experimental space for biological cell lysis preparation, a closed space is formed between the upper frame 11 and the mounting plate 13, which is the main working space. The target bottle remains sealed when entering the tooling device, and is only opened for liquid injection inside the device to prevent foreign matter from falling into the target bottle and affecting the experimental results. The mounting plate 13 is the main load-bearing plate. In order to prevent positioning deviation caused by collision and vibration, the lower frame 12 and the mounting plate 13 are tightly connected. In order to facilitate the detection of the working condition of the special machine, an alarm light 14 is set on the top. When the equipment fails or the operation is incorrect, the alarm light 14 flashes.

[0039] like Figures 1 to 4As shown, the loading and unloading device 2 includes a loading component 21, a transmission component 22 and a wire protection chain 24. A plurality of safety baffles 23 are provided on the upper end of the loading component 21. The loading component 21 is fastened to the mounting plate 13. The transmission component 22 is fastened to one side of the wire protection chain 24. The loading component 21 includes a loading cylinder 211, a loading stacker 212, a transmission block 213 and a stacking bracket 214. One side of the loading cylinder 211 is fastened to the stacking bracket 214. The output end of the loading cylinder 211 is fastened to the transmission block 213. The loading stacker 212 is fastened to the transmission block 213. There are feeding guide rails 215 on both sides of the bottom, and a slider is provided on the top of the stacking bracket 214. The slider of the stacking bracket 214 is slidably connected to the feeding guide rail 215. The middle of the bottom of the feeding stacking 212 is fastened to the transmission block 213. The transmission component 22 is fastened to the mounting plate 13. The transmission component 22 includes a sliding cylinder 221, a lead screw 222, a nut 223, a bearing seat 224, a transmission bracket 225, a transmission chuck 226, a chuck fixing plate 227, a transmission cylinder 228 and a transmission slider 229. One side of the sliding cylinder 221 is connected to the transmission The bracket 225 is fastened, the output end of the sliding cylinder 221 is fastened to one side of the lead screw 222, the two ends of the lead screw 222 are movably connected to the bearing seat 224, one end of the transmission bracket 225 is provided with a mounting cavity, the bearing seat 224 and the inner wall of the mounting cavity of the transmission bracket 225 are fastened, the nut 223 is threadedly connected to the lead screw 222, the outer cylindrical surface of the nut 223 is fastened to one side of the transmission slider 229, one side of the transmission bracket 225 is fastened to the wire protection chain 24, the bottom end of the transmission bracket 225 is fastened to the mounting plate 13, and the transmission bracket 225 is fastened to the mounting plate 13. A transmission guide rail 2251 is provided on one side, and the transmission slider 229 is slidably connected to the transmission guide rail 2251. A guide rail is provided on one side of the transmission slider 229, and the guide rail of the transmission slider 229 and the transmission guide rail 2251 are perpendicular to each other. A slider is provided on one side of the chuck fixing plate 227, and the slider of the chuck fixing plate 227 is slidably connected to the guide rail of the transmission slider 229. One side of the transmission cylinder 228 is tightly connected to the transmission slider 229, the output end of the transmission cylinder 228 is tightly connected to the chuck fixing plate 227, and the chuck fixing plate 227 and the transmission chuck 226 are tightly connected.

[0040] Safety baffles 23 are arranged around the feeding assembly 21 to prevent the target bottle from tilting during transfer and installation. The feeding assembly 21 and the mounting plate 13 are tightly connected to reduce the influence of the working vibration of the feeding cylinder 211 on the feeding. The power line and the control line are fixed uniformly by the wire protection chain 24. In order to prevent the line from interfering with the movement, the wire protection chain 24 is imprisoned at one end of the transmission bracket 225. With the movement of the nut 223, the wire protection chain 24 is free to expand and contract. In order to improve the efficiency of culture fluid infusion, a number of target bottles are neatly placed on the feeding stack 212, and are automatically picked up by the transmission chuck 226. One side of the feeding cylinder 211 is fastened to the stacking bracket 214, the feeding cylinder 211 is installed and positioned, the output end of the feeding cylinder 211 is fastened to the transmission block 213, the transmission block 213 is controlled to reciprocate through the movement of the cylinder, and the middle of the bottom end of the feeding stacking 212 is fastened to the transmission block 213. In order to accurately control the movement of the feeding stacking 212, guide rails and sliders are added, and the movement of the feeding stacking 212 is guided by the sliding connection. As the target bottle near the side of the transmission chuck 226 is filled and sealed, the feeding stacking 212 is controlled by the cylinder to move to the side of the transmission chuck 226 Move until all target bottles have been infused and the alarm light 14 lights up before replacement. Since a number of target bottles are arranged vertically and horizontally on the loading stack 212, the transmission chuck 226 is controlled by the sliding cylinder 221 to move horizontally. In order to facilitate transmission, the sliding cylinder 221 is installed on one side of the transmission bracket 225, and the output end and the lead screw 222 are tightly connected. The positioning control is more accurate through threaded engagement, and the number of adjustments of the transmission chuck 226 is reduced. Two bearing seats 224 are installed in the installation cavity of the transmission bracket 225, and the lead screw 222 is movably connected through the built-in bearing and the two ends of the lead screw 222. 2 can rotate along the axis and control the transmission slider 229 fastened with the nut 223 to move in the horizontal plane. When it moves to the top of the target bottle, the transmission cylinder 228 controls the chuck fixing plate 227 to move in the vertical direction until the chuck touches the target bottle mouth and clamps the target bottle. Then the transmission cylinder 228 controls the chuck to move up, and controls the target bottle to move horizontally to the next station through the sliding cylinder 221. The target bottles of different heights can be picked up through the vertical movement, which is more practical. Through the fastening connection between the components, the control accuracy of the control machine is higher and the processing efficiency is further improved.

[0041] like Figures 5-6As shown, the horizontal shaking device 4 includes a horizontal shaking bracket 41, a horizontal shaking beam 42, a horizontal shaking slider 43, a horizontal shaking cylinder 44, a horizontal turntable 45, a first motor 46, a first guide column 48 and a first slider 49. The top of the horizontal shaking bracket 41 is tightly connected to one side of the horizontal shaking beam 42, the bottom of the horizontal shaking bracket 41 is tightly connected to the mounting plate 13, a horizontal shaking guide rail 421 is provided on one side of the horizontal shaking beam 42, the horizontal shaking slider 43 is slidably connected to the horizontal shaking guide rail 421, the top of the horizontal shaking slider 43 is tightly connected to the horizontal shaking cylinder 44, and one side of the horizontal shaking slider 43 is provided with a guide rail perpendicular to the horizontal shaking The vertical guide rail of the guide rail 421, a slider is provided on one side of the first motor 46, the slider of the first motor 46 is slidably connected to the vertical guide rail of the horizontal liquid shaking slider 43, the output end of the horizontal liquid shaking cylinder 44 is tightly connected to the slider of the first motor 46, the output end of the first motor 46 is tightly connected to the horizontal turntable 45, a plurality of horizontal liquid shaking chucks 47 are provided on the bottom surface of the horizontal turntable 45, a guide column mounting groove is provided on one side of the horizontal liquid shaking beam 42, the first guide column 48 is tightly connected to the end face of the guide column mounting groove of the horizontal liquid shaking beam 42, the first slider 49 is slidably connected to the first guide column 48, and the first slider 49 is tightly connected to the horizontal liquid shaking slider 43 on the side away from the first guide column 48.

[0042] The horizontal shaking device 4 is installed on one side of the mounting plate 13. In order to facilitate the picking up of the target bottle, two sets of guide rail slider mechanisms are arranged on the horizontal shaking device 4 to guide the movement. In order to prevent the horizontal shaking from colliding with other parts, the target bottle is moved horizontally away from the screw-on manipulator 6 through the horizontally arranged guide rail slider mechanism, and the target bottles of different heights are picked up through the vertically arranged guide rail slider mechanism. Since the linear displacement of the horizontal shaking chuck 47 is controlled by the cylinder, the influence of vibration on the displacement is reduced by tightening the connection. In order to improve the integration performance of the horizontal shaking device 4, a guide rail is arranged on the horizontal shaking slider 43, and a slider is arranged on one side of the first motor 46. The output end of the horizontal shaking cylinder 44 and the first motor The 46 sliders are tightly connected to accurately control the up and down movement of the first motor 46. The output end of the first motor 46 is tightly connected to the horizontal turntable 45. The horizontal turntable 45 is controlled to rotate along the axis by the rotation of the first motor 46. The culture solution in the target bottle is gradually mixed by rotation. A plurality of horizontal liquid shaking chucks 47 are provided on the lower surface of the horizontal turntable 45 to control the rotation of multiple target bottles to improve work efficiency. The horizontal liquid shaking chucks 47 are controlled by small cylinders to clamp the bottle mouths of the target bottles. Each horizontal liquid shaking chuck 47 is equipped with a small cylinder to control the horizontal liquid shaking chuck 47 individually. A guide column slider mechanism is added to the horizontal liquid shaking beam 42 to cooperate with the guide rail slider mechanism to perform bidirectional positioning for horizontal movement, so that the displacement accuracy is higher.

[0043] like Figures 7 to 9As shown, the vertical shaking liquid device 5 includes a vertical shaking liquid bracket 51, a vertical shaking liquid beam 52, a vertical shaking liquid slider 53, a vertical shaking liquid cylinder 54, a vertical turntable 55, a second motor 56, a second guide column 58 and a second slider 59. The top of the vertical shaking liquid bracket 51 is tightly connected to one side of the vertical shaking liquid beam 52, the bottom of the vertical shaking liquid bracket 51 is tightly connected to the mounting plate 13, a vertical shaking liquid guide rail 521 is provided on one side of the vertical shaking liquid beam 52, the vertical shaking liquid slider 53 is slidably connected to the vertical shaking liquid guide rail 521, one side of the vertical shaking liquid slider 53 is tightly connected to the vertical shaking liquid cylinder 54, and one side of the vertical shaking liquid slider 53 is provided with a vertical shaking liquid guide rail 521. The vertical guide rail of the guide rail 521, a slider is provided on one side of the second motor 56, the slider of the second motor 56 is slidably connected to the vertical guide rail of the vertical liquid shaking slider 53, the output end of the vertical liquid shaking cylinder 54 is tightly connected to the slider of the second motor 56, the output end of the second motor 56 is tightly connected to the vertical turntable 55, a plurality of vertical liquid shaking chucks 57 are provided on the bottom surface of the vertical turntable 55, a guide column mounting groove is provided on one side of the vertical liquid shaking beam 52, the second guide column 58 is tightly connected to the end face of the guide column mounting groove of the vertical liquid shaking beam 52, the second slider 59 is slidably connected to the second guide column 58, and the second slider 59 is tightly connected to the vertical liquid shaking slider 53 on the side away from the second guide column 58.

[0044] After the horizontal shaking, the target bottle is sent to the screwing manipulator 6, which screws off the target bottle cap. As the rotating disk 74 rotates, the target bottle is injected with culture solution again by the injection manipulator 8. After the second injection, the bottle cap is tightened by the screwing manipulator 6. As the components of the culture solution increase, the horizontal shaking can no longer make the culture solution more uniform. The vertical shaking device 5 is used for further mixing. After two mixings, the culture solution is no longer stratified. The vertical shaking support 51 and the vertical shaking horizontal support 52 are connected by fastening. The connection between the beam 52 and the mounting plate 13 is tighter, which weakens the positioning error caused by external collision. A vertical liquid shaking guide rail 521 is provided on one side of the vertical crossbeam, which is slidably connected with the vertical liquid shaking slider 53 to guide the horizontal displacement, and is bidirectionally positioned by the guide column slider mechanism to improve the positioning accuracy. One side of the vertical liquid shaking cylinder 54 is tightly connected to the vertical liquid shaking slider 53, so that the vertical displacement mechanism moves horizontally with the movement of the slider, and then the vertical liquid shaking guide rail 521 provided on the vertical liquid shaking slider 53 is used to make the first The second motor 56 moves in the vertical direction. As the second motor 56 moves downward, several vertical shaking liquid chucks 57 arranged on the vertical turntable 55 touch the target bottle mouth. The vertical shaking liquid chucks 57 are controlled by a small cylinder to clamp the bottle mouth of the target bottle. In order to prevent the target bottle from colliding with other devices when the vertical turntable 55 rotates, the chuck height is increased by a vertically arranged guide slider mechanism, and the vertical turntable 55 is away from the screw-on manipulator 6 by a horizontally arranged guide slider mechanism. The second motor 56 drives the vertical turntable 55 to rotate along the axis, and the target bottle rotates accordingly. The culture solution in the target bottle is flipped and mixed in the vertical plane, further improving the mixing degree of the culture solution in the target bottle. There are two vertical shaking liquid devices 5, which pick up the target bottles alternately, shortening the waiting time for the vertical shaking liquid chucks 57 to clamp the target bottle. Several vertical shaking liquid chucks 57 are arranged under the vertical turntable 55, and can continuously pick up the target bottle as the turntable rotates. Then, they are driven by the second motor 56 to shake the liquid for multiple target bottles at the same time, greatly improving the efficiency of shaking the liquid.

[0045] like Figure 1 , 10 As shown, the power transmission device 7 includes a driving motor 71, a reducer 72, a flange turntable 73, a rotating disk 74, a reducer bracket 74 and an auxiliary rotating assembly 76. One side of the driving motor 71 is rotatably connected to the reducer 72. The reducer 72 and the reducer bracket 74 are tightly connected. The reducer bracket 74 extends upward and is provided with a support column. The support column of the reducer bracket 74 is tightly connected to the mounting plate 13. The reducer 72 is tightly connected to the flange turntable 73. The bottom end of the flange turntable 73 is tightly connected to the rotating disk 74. A plurality of auxiliary rotating assemblies 76 are provided on the lower surface of the rotating disk 74. The auxiliary rotating assembly 76 includes a rotating wheel 761 and a rotating wheel bracket 762. The rotating wheel 761 and the rotating wheel bracket 762 are movably connected.

[0046] The present invention uses the drive motor 71 as the main power source of the power transmission device 7. In order to improve the stability between the various components, the drive motor 71 is directly connected to the rotating disk 74 through a tightening connection to reduce the influence of vibration on the torque transmission. Because the speed of the drive motor 71 is too fast, it will cause unstable force transmission and inconvenience in rotation. Therefore, the output speed is reduced by the reducer 72, and the reduced torque is transmitted to the rotating disk 74 through the flange turntable 73. Since the contact surface between the flange turntable 73 and the rotating disk 74 is relatively large, the torque output is more stable. In order to reduce the radial force on the output shaft of the reducer 72 when the rotating disk 74 rotates, a plurality of auxiliary rotating components 76 are arranged in the circumferential direction at the bottom end of the rotating disk 74, and are installed on the rotating disk 74 through the turntable bracket 762. As the rotating disk 74 rotates, the turntable 761 rolls on the surface of the mounting plate 13, supporting the rotating disk 74, and further improving the stability of the rotation of the rotating disk 74.

[0047] The biological cell lysis preparation workstation also includes a liquid removal robot 3, the bottom end of which is tightly connected to the mounting plate 13, a plurality of liquid injection robots 8 are arranged on the mounting plate 13, the liquid injection robots 8 are tightly connected to the mounting plate 13, and a plurality of screw-on robots 6 are arranged along the circumferential direction on one side of the rotating disk 74, the bottom end of the screw-on robot 6 is tightly connected to the rotating disk 74.

[0048] After the target bottle is sent to the screw-on robot 6 for uncapping, the target bottle is clamped out by the liquid removal robot 3, and the target bottle is inverted to remove the residual liquid in the target bottle after cleaning, and the remaining liquid is collected by the receiving plate at the bottom of the liquid removal robot 3. A plurality of liquid injection robots 8 can be configured with multi-component culture medium. Each robot controls the cylinder to work through a controller, and the vibration is relatively large. The work is smoother through a fastening connection. In order to improve work efficiency, a plurality of screw-on robots 6 are arranged on the rotating disk 74, so that each workstation corresponds to at least one screw-on robot 6, reducing the transmission path between adjacent workstations and improving the injection efficiency. In addition to performing the screw-on operation, the screw-on robot 6 also has the function of spraying codes on the target bottle, which is convenient for distinguishing the target bottles, especially when configuring large quantities of culture medium, greatly improving the classification efficiency.

[0049] Working principle of the present invention: The present invention performs mechanized batch configuration of target bottles for biological cell lysis preparation to replace manual configuration, automatically feeds the target bottles through the loading and unloading device 2, and adds a horizontal shaking device 4 and a vertical shaking device 5 on the upper surface of the mounting plate 13. The culture solution is evenly mixed by alternately rotating the horizontal and vertical surfaces, and a closed space is formed between the upper frame 11 and the mounting plate 13. The target bottles remain sealed when entering the tooling device, and only the opening for liquid injection is performed in the device; two sets of guide rails are arranged on the horizontal shaking device 4. The block mechanism is used to move the target bottle in the horizontal direction away from the screw-on robot 6 through the horizontally arranged guide rail slider mechanism, and other parts will not be collided during horizontal shaking. The target bottles at different heights are picked up through the vertically arranged guide rail slider mechanism. A plurality of auxiliary rotating components 76 are arranged along the circumferential direction at the bottom end of the rotating disk 74. As the rotating disk 74 rotates, the rotating wheel 761 rolls on the surface of the mounting plate 13 to support the rotating disk 74. In addition to the capping operation, the screw-on robot 6 also has the function of spraying codes on the target bottles to classify the target bottles.

[0050] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0051] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A biological cell lysis preparation workstation, characterized in that: The preparation workstation comprises a box assembly (1), a loading and unloading device (2), a horizontal liquid shaking device (4), a vertical liquid shaking device (5) and a power transmission device (7); the loading and unloading device (2) and the box assembly (1) are tightly connected; the box assembly comprises a mounting plate (13); the horizontal liquid shaking device (4) is mounted on one side of the upper end of the mounting plate (13); the vertical liquid shaking device (5) is mounted on one side of the upper end of the mounting plate (13) away from the horizontal liquid shaking device (4); and the power transmission device and the mounting plate (13) are tightly connected; The loading and unloading device (2) comprises a loading assembly (21), a transmission assembly (22) and a wire protection chain (24); a plurality of safety baffles (23) are provided at the upper end of the loading assembly (21); the loading assembly (21) is tightly connected to the mounting plate (13); the transmission assembly (22) is tightly connected to one side of the wire protection chain (24); the loading assembly (21) comprises a loading cylinder (211), a loading stacker (212), a transmission block (213) and a stacker bracket (214); ), one side of the feeding cylinder (211) is tightly connected to the stacking bracket (214), the output end of the feeding cylinder (211) is tightly connected to the transmission block (213), both sides of the bottom end of the feeding stacking (212) are provided with feeding guide rails (215), the top of the stacking bracket (214) is provided with a slide block, the slide block of the stacking bracket (214) is slidably connected to the feeding guide rail (215), and the middle of the bottom end of the feeding stacking (212) is tightly connected to the transmission block (213); The horizontal shaking device (4) comprises a horizontal shaking support (41), a horizontal shaking beam (42), a horizontal shaking slider (43), a horizontal shaking cylinder (44), a horizontal turntable (45), a first motor (46), a first guide column (48) and a first slider (49); the top of the horizontal shaking support (41) is tightly connected to one side of the horizontal shaking beam (42); the bottom of the horizontal shaking support (41) is tightly connected to a mounting plate (13); a horizontal shaking guide rail (421) is provided on one side of the horizontal shaking beam (42); the horizontal shaking slider (43) is slidably connected to the horizontal shaking guide rail (421); the top of the horizontal shaking slider (43) is tightly connected to the horizontal shaking cylinder (44); one side of the horizontal shaking slider (43) is provided with a guide rail perpendicular to the horizontal shaking guide rail (421), a slider is provided on one side of the first motor (46), the slider of the first motor (46) is slidably connected to the vertical guide rail of the horizontal liquid shaking slider (43), the output end of the horizontal liquid shaking cylinder (44) is tightly connected to the slider of the first motor (46), the output end of the first motor (46) is tightly connected to the horizontal turntable (45), a plurality of horizontal liquid shaking chucks (47) are provided on the bottom surface of the horizontal turntable (45), a guide column mounting groove is provided on one side of the horizontal liquid shaking beam (42), the first guide column (48) is tightly connected to the end face of the guide column mounting groove of the horizontal liquid shaking beam (42), the first slider (49) is slidably connected to the first guide column (48), and the side of the first slider (49) away from the first guide column (48) is tightly connected to the horizontal liquid shaking slider (43); The vertical shaking device (5) comprises a vertical shaking support (51), a vertical shaking beam (52), a vertical shaking slider (53), a vertical shaking cylinder (54), a vertical turntable (55), a second motor (56), a second guide column (58) and a second slider (59); the top of the vertical shaking support (51) is tightly connected to one side of the vertical shaking beam (52); the bottom of the vertical shaking support (51) is tightly connected to a mounting plate (13); a vertical shaking guide rail (521) is provided on one side of the vertical shaking beam (52); the vertical shaking slider (53) is slidably connected to the vertical shaking guide rail (521); one side of the vertical shaking slider (53) is tightly connected to the vertical shaking cylinder (54); one side of the vertical shaking slider (53) is provided with a guide rail perpendicular to the vertical shaking guide rail. (521), a slider is provided on one side of the second motor (56), the slider of the second motor (56) is slidably connected to the vertical guide rail of the vertical liquid shaking slider (53), the output end of the vertical liquid shaking cylinder (54) is tightly connected to the slider of the second motor (56), the output end of the second motor (56) is tightly connected to the vertical turntable (55), a plurality of vertical liquid shaking chucks (57) are provided on the bottom surface of the vertical turntable (55), a guide column mounting groove is provided on one side of the vertical liquid shaking beam (52), the second guide column (58) is tightly connected to the end face of the guide column mounting groove of the vertical liquid shaking beam (52), the second slider (59) is slidably connected to the second guide column (58), and the side of the second slider (59) away from the second guide column (58) is tightly connected to the vertical liquid shaking slider (53); The power transmission device (7) comprises a driving motor (71), a reducer (72), a flange rotating disk (73), a rotating disk (74), a reducer bracket (75) and an auxiliary rotating assembly (76); one side of the driving motor (71) is rotatably connected to the reducer (72); the reducer (72) and the reducer bracket (75) are tightly connected; the reducer bracket (75) is upwardly extended with a support column; the support column of the reducer bracket (75) is tightly connected to the mounting plate (13); the reducer (72) and the flange rotating disk (73) are tightly connected; the bottom end of the flange rotating disk (73) and the rotating disk (74) are tightly connected; a plurality of auxiliary rotating assemblies (76) are provided on the lower surface of the rotating disk (74); the auxiliary rotating assembly (76) comprises a rotating wheel (761) and a rotating wheel bracket (762); the rotating wheel (761) and the rotating wheel bracket (762) are movably connected; The loading and unloading device (2) automatically feeds the target bottle, the horizontal shaking device (4) picks up the target bottle through the horizontal shaking chuck (47) and rotates on a horizontal plane to mix the culture solution injected in batches, the vertical shaking device (5) rotates and shakes the target bottle on a vertical plane, and the power transmission device (7) and the mounting plate (13) are tightly connected to ensure smooth torque transmission.

2. A biological cell lysis preparation workstation according to claim 1, characterized in that: The box assembly (1) further comprises an upper frame (11), a lower frame (12) and an alarm light (14); one side of the upper frame (11) is sealedly connected to the mounting plate (13); one side of the lower frame (12) is tightly connected to a side of the mounting plate (13) away from the upper frame (11); and the alarm light (14) is mounted on the upper end of the upper frame (11).

3. A biological cell lysis preparation workstation according to claim 1, characterized in that: The transmission component (22) is tightly connected to the mounting plate (13). The transmission component (22) comprises a sliding cylinder (221), a lead screw (222), a nut (223), a bearing seat (224), a transmission bracket (225), a transmission chuck (226), a chuck fixing plate (227), a transmission cylinder (228) and a transmission slider (229). One side of the sliding cylinder (221) is tightly connected to the transmission bracket (225). The output end of the sliding cylinder (221) is tightly connected to one side of the lead screw (222). Both ends of the lead screw (222) are movably connected to the bearing seat (224). One end of the transmission bracket (225) is provided with a mounting cavity. The bearing seat (224) is tightly connected to the inner wall of the mounting cavity of the transmission bracket (225). The nut (223) is threadedly connected to the lead screw (222). The outer cylindrical surface of the nut (223) and the transmission slider (2 29) one side is fastened and connected, one side of the transmission bracket is fastened and connected to the wire protection chain (24), the bottom end of the transmission bracket (225) is fastened and connected to the mounting plate (13), one side of the transmission bracket (225) is provided with a transmission guide rail (2251), the transmission slider (229) is slidably connected to the transmission guide rail (2251), one side of the transmission slider (229) is provided with a guide rail, the guide rail of the transmission slider (229) and the transmission guide rail (2251) are perpendicular to each other, one side of the chuck fixing plate (227) is provided with a slider, the slider of the chuck fixing plate (227) is slidably connected to the guide rail of the transmission slider (229), one side of the transmission cylinder (228) is fastened and connected to the transmission slider (229), the output end of the transmission cylinder (228) is fastened and connected to the chuck fixing plate (227), and the chuck fixing plate (227) is fastened and connected to the transmission chuck (226).

4. A biological cell lysis preparation workstation according to claim 1, characterized in that: The preparation workstation further comprises a liquid removal manipulator (3), the bottom end of the liquid removal manipulator (3) is tightly connected to the mounting plate (13), a plurality of liquid injection manipulators (8) are arranged on the mounting plate (13), the liquid injection manipulators (8) are tightly connected to the mounting plate (13), and a plurality of screw-on manipulators (6) are arranged along the circumferential direction on one side of the rotating disk (74), the bottom end of the screw-on manipulator (6) is tightly connected to the rotating disk (74).

Citation Information

Patent Citations

  • Biological cell lysis preparation workstation

    CN215050218U