Fully automatic chemical analysis and detection device and detection method

Abstract

The present invention discloses a fully automatic chemical analysis and detection device and a detection method. It is mainly used to realize fully automatic chemical analysis processes such as sampling, reagent addition, mixing reaction, digestion, extraction, separation and detection, so as to save human resources, reduce reagent consumption and waste liquid discharge in the chemical detection process, and improve the analysis and detection speed, precision and accuracy in the fields of environment, food and biochemistry.

Description

Technical Field

[0001] The present invention relates to analytical and detection instruments, and particularly to a fully automatic chemical analysis and detection device. Background Art

[0002] With the development of society and the improvement of living standards, the requirements of human beings for the quality of the living environment and food are also increasing day by day, and the awareness of environmental protection is gradually enhanced. Therefore, the amount of samples that need to be analyzed and detected daily in the fields of agriculture, environment, food, and medicine and health is increasing day by day. To improve the detection speed and accuracy of samples, scientists have developed various automated chemical analysis techniques such as bubble interval analysis technique, flow injection analysis technique, sequential injection technique, and fully automatic discontinuous chemical analysis technique. The bubble interval analysis technique and the flow injection analysis technique are automated analysis techniques in which samples and reagents are continuously mixed and reacted in a closed pipeline and then transported to a detector for detection. When the analyzers based on these two techniques are operating, reagents need to be continuously consumed to form a stable baseline, so it is necessary to always pay attention to the signal changes of the baseline, and the bubbles that accidentally enter or are generated will generate interference signals, resulting in invalid detection results. Although the sequential injection technique avoids the continuous consumption of reagents and the amounts of samples and reagents used are very small, since the samples and reagents are sequentially injected into the reaction coil for mixing and reaction, the mixing effect for chemical reactions using less than 2 reagents can still meet the requirements, but for chemical reactions that require more than 3 reagents, the mixing effect and detection speed will be greatly reduced, which is also one of the reasons why the commercial instruments based on this technique have not been widely used. The fully automatic discontinuous chemical analysis technique almost simulates manual analysis operations. Samples are placed in a series of reaction vessels, and then reagents are sequentially injected into the reaction vessels. After thorough mixing and reaction, the detection is directly carried out in the reaction vessels or the solution is transferred to a flowing detection cell for detection. The instruments of this type have complex structures and large volumes, and currently cannot perform pretreatment operations such as automatic digestion, extraction, and separation. Additional instruments and equipment are required, and the degree of automation still needs to be improved. Summary of the Invention

[0003] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a fully automatic chemical analysis and detection device and a detection method.

[0004] One of the technical solutions adopted by the present invention to solve its technical problems is:

[0005] A fully automatic chemical analysis and detection device includes: a sample injection module, a pretreatment module, a mixing and separation module, a detection module, a two-way power module, a multi-channel connection module, a three-way connection module, a five-way connection module, and pipelines connecting all modules; wherein,

[0006] The sample injection module includes a number of reagent bottles arranged in parallel, and samples, reaction reagents, and cleaning solutions are respectively contained in the number of reagent bottles;

[0007] The pretreatment module includes a temperature-controlled reactor, an ultraviolet digester, and a pretreatment column that are arranged in parallel.

[0008] The mixing and separation module includes a mixing separator; the interior of the mixing separator has a spindle-shaped cavity, and the mixing separator is provided with a bottom interface, a top interface, and a mid-upper interface that communicate with the cavity.

[0009] The detection module includes a detector and a waste liquid bottle that are arranged in series.

[0010] The bidirectional power module communicates with one end of the multi-channel connection module and the bottom interface of the mixing separator.

[0011] One end of the multi-channel connection module communicates with the bidirectional power module, and the other end of the multi-channel connection module selectively communicates with the plurality of reagent bottles, the temperature-controlled reactor, the ultraviolet digester, the pretreatment column, the five-way connection module, and the detection module.

[0012] One end of the three-way connection module communicates with the five-way connection module, and the other end of the three-way connection module selectively communicates with the waste liquid bottle of the detection module and the mid-upper interface of the mixing separator.

[0013] One end of the five-way connection module communicates with the three-way connection module, and the other end of the five-way connection module selectively communicates with the temperature-controlled reactor, the ultraviolet digester, the pretreatment column, and the multi-channel connection module.

[0014] In one embodiment: the multi-channel connection module is a multi-position selection valve, and the common interface of the multi-position selection valve selectively communicates with a plurality of branch interfaces of the multi-position selection valve; the common interface of the multi-position selection valve communicates with the bidirectional power module, and the plurality of branch interfaces respectively communicate with the plurality of reagent bottles, the temperature-controlled reactor, the ultraviolet digester, the pretreatment column, the five-way connection module, and the detection module.

[0015] In one embodiment: the multi-position selection valve is a four-position, six-position, eight-position, ten-position, twelve-position, fourteen-position, or sixteen-position selection valve.

[0016] In one embodiment: the three-way connection module is a three-way valve, and the common interface of the three-way valve selectively communicates with the first interface of the three-way valve and the second interface of the three-way valve; the common interface of the three-way valve communicates with the five-way connection module, the first interface of the three-way valve communicates with the waste liquid bottle, and the second interface of the three-way valve communicates with the mid-upper interface of the mixing separator.

[0017] In one embodiment, the five-way connection module is a five-way joint. The common interface of the five-way joint is connected to the three-way connection module. The first interface of the five-way joint is connected to the temperature-controlled reactor. The second interface of the five-way joint is connected to the ultraviolet digestion device. The third interface of the five-way joint is connected to the pretreatment column. The fourth interface of the five-way joint is connected to the multi-channel connection module.

[0018] In one embodiment, the multi-channel connection module is a plurality of switching valves or a plurality of three-way valves arranged in parallel.

[0019] In one embodiment, the three-way connection module is two switching valves arranged in parallel.

[0020] In one embodiment, the middle section of the cavity of the mixing separator is tubular, with a diameter of 3 mm to 30 mm and a height of 3 mm to 70 mm. The two ends of the cavity are conical, with a height of 3 mm to 50 mm.

[0021] In one embodiment, the distance between the middle-upper interface and the top interface is 0 to 10 mm.

[0022] In one embodiment, the mixing and separation module further includes an air filter, and the air filter is connected to the top interface of the mixing separator.

[0023] In one embodiment, the two-way power module includes a two-way pump, and the two-way pump is a peristaltic pump or a multi-plunger pump with a positive and reverse rotation function.

[0024] In one embodiment, the detector includes a spectrophotometer with a flow-through detection cell, a fluorescence photometer, an atomic absorption photometer, an inductively coupled plasma emission spectrometer, an inductively coupled plasma mass spectrometer, or an electrochemical detector, etc.

[0025] In one embodiment, the pretreatment column includes a solid-phase extraction column, an on-line oxidation or reduction column, etc.

[0026] The second technical solution adopted by the present invention to solve its technical problems is:

[0027] A detection method using the above-mentioned fully automatic chemical analysis and detection device, including:

[0028] 1) The multi-channel connection module is switched to the sample bottle containing the sample, and the sample is sent into the mixing separator through the two-way power module; then step 2) is performed, or step 3) is directly performed;

[0029] 2) The multi-channel connection module is switched to the pretreatment module, and the liquid in the mixing separator is sent into the pretreatment module through the two-way pump for pretreatment; after the pretreatment is completed, the liquid is sent back to the mixing separator through the two-way pump; then step 3) is performed, or step 4) is directly performed;

[0030] 3) The multi-channel connection module switches to each reagent bottle in sequence, and sends each reaction reagent into the mixing separator through the bidirectional power module; then repeat step 2) and then proceed to step 4), or directly proceed to step 4);

[0031] 4) Perform cyclic mixing reaction; then repeat step 2) or 3), or directly proceed to step 5);

[0032] 5) Send the reaction solution into the detector for detection through the bidirectional power module;

[0033] 6) After the detection is completed, the multi-channel connection module switches to the reagent bottle filled with the cleaning solution, and sends the cleaning solution into the mixing separator through the bidirectional power module; then the multi-channel connection module switches to each module in sequence, and sends the cleaning solution in the mixing separator into each module in sequence through the bidirectional power module for cleaning.

[0034] For the equipment, reagents, processes, parameters, etc. involved in the present invention, unless otherwise specified, they are all conventional equipment, reagents, processes, parameters, etc., and no embodiments will be given anymore.

[0035] All ranges listed in the present invention include all point values within that range.

[0036] The "about", "approximate" or "around" in the present invention refers to within the range of ±20% of the specified range or value.

[0037] In the present invention, the "room temperature" is the conventional ambient temperature and can be 10 - 30°C.

[0038] Compared with the background technology, the present technical solution has the following advantages:

[0039] 1. The fully automatic chemical analysis and detection device of the present invention integrates pretreatment modules commonly used in chemical analysis such as a temperature-controlled reactor, an ultraviolet digester, and a pretreatment column, and has a small volume and a high degree of integration;

[0040] 2. The fully automatic chemical analysis and detection device of the present invention is provided with a unique mixing separator, which is small in size and can not only play a mixing role in chemical reactions but also serve as a separator during liquid-liquid extraction;

[0041] 3. The fully automatic chemical analysis and detection device of the present invention consumes less reagents and does not need to continuously inject reagents during operation, which is beneficial to energy conservation, emission reduction and reducing the cost of analysis and testing;

[0042] 4. The fully automatic chemical analysis and detection device of the present invention operates flexibly. The reaction time can adjust the mixing time according to the concentration of the target substance, and the analysis process is programmed and controlled with a high degree of automation;

[0043] 5. The fully automatic chemical analysis and detection device of the present invention can analyze and detect multiple target substances through one device, which is beneficial to reducing the instrument cost and saving laboratory space. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 It is a schematic flow path diagram of the fully automatic chemical analysis and detection device according to the embodiment of the present invention.

[0045] Figure 2 It is a schematic flow path diagram of a solution that uses multiple parallel switching valves to replace the multi-position selection valve in the embodiment.

[0046] REFERENCE SIGNS:

[0047] Multi-position selection valve 1, common interface 1-0 of the multi-position selection valve, branch interfaces a / b / c / d / e / f / g / h / i / j / k / l of the multi-position selection valve; switching valves a' / b' / c' / d' / e' / f' / g' / h' / i' / j' / k' / l' of the alternative solution;

[0048] Two-way pump 2;

[0049] Mixing and separating device 3, bottom interface 3-1, top interface 3-2, middle upper interface 3-3, cavity 3-4;

[0050] Three-way valve 4, common interface 4-0 of the three-way valve, first interface 4-1 of the three-way valve, second interface 4-2 of the three-way valve;

[0051] Detector 5;

[0052] Five-way connection 6, common interface 6-0 of the five-way connection, first interface 6-1 of the five-way connection, second interface 6-2 of the five-way connection, third interface 6-3 of the five-way connection, fourth interface 6-4 of the five-way connection;

[0053] Temperature-controlled reactor 7; Ultraviolet digestion device 8; Pretreatment column 9; Air filter 10;

[0054] Sample bottle 11; Reaction reagent bottles 12-1 / 12-2 / 12-3 / 12-4 / 12-5; Cleaning liquid bottle 13; Waste liquid bottle 14. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0055] The present invention will be further described below with reference to the drawings and embodiments.

[0056] In the description of the present invention, it should be understood that the orientation or positional relationships indicated by the terms "upper", "lower", "front", "rear", "left", "right", "horizontal", "vertical", "top", "bottom", "inner", "outer", etc. are based on the orientation or positional relationships shown in the three-dimensional view in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation to the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0057] Embodiment

[0058] As Figure 1 shown, the fully automatic chemical analysis and detection device of this embodiment includes a multi-position selection valve 1, a two-way pump 2, a mixing and separator 3, a three-way valve 4, a detector 5, a five-way joint 6, a temperature-controlled reactor 7, an ultraviolet digester 8, a pretreatment column 9, an air filter 10, a sample bottle 11, various reaction reagent bottles 12-1 / 12-2 / 12-3 / 12-4 / 12-5, a cleaning solution bottle 13, and a waste liquid bottle 14.

[0059] The multi-position selection valve 1 can be a four-way, six-way, eight-way, ten-way, twelve-way, fourteen-way or sixteen-way selection valve, which includes a common interface 1-0 and a plurality of branch interfaces a / b / c / d / e / f / g / h / i / j / k / l surrounding it. Each time, only one of the branch interfaces a / b / c / d / e / f / g / h / i / j / k / l is in communication with the common interface 1-0. The multi-position selection valve 1 in this embodiment can also be replaced by a plurality of parallel switching valves a’ / b’ / c’ / d’ / e’ / f’ / g’ / h’ / i’ / j’ / k’ / l’ or three-way valves to achieve the effect of selecting a single channel. The flow path schematic diagram is as Figure 2 shown.

[0060] The two-way pump 2 can be a peristaltic pump or a multi-plunger pump with forward and reverse functions, and the flow rate range is 0.01 mL / min to 1 L / min.

[0061] The mixing separator 3 adopts a cylindrical or cuboid module with a cavity 3-4 inside that is similar to a spindle shape. The main body of the internal cavity 3-4 is a tubular cavity with a diameter of 3 mm to 30 mm, a height of 3 mm to 70 mm. The two ends of the cavity are two cones of the same size, with a height of 3 mm to 50 mm. The mixing separator 3 is installed in a vertical state and includes a bottom interface 3-1, a top interface 3-2, and a middle-upper interface 3-3 near the top. Among them, the distance between the middle-upper interface 3-3 and the top interface 3-2 is 0 to 10 mm. The mixing separator 3 can be made of glass or quartz, or can be processed from inert plastics such as polytetrafluoroethylene, plexiglass, or polyether ether ketone.

[0062] The three-way valve 4 is an electromagnetic three-way valve, an electromagnetic pinch valve, or a motor-driven rotary three-way valve, and includes a common interface 4-0 and a first interface 4-1 and a second interface 4-2 that are selectively connected to the common interface 4-0. The three-way valve 4 in this embodiment can also be replaced by 2 on-off valves to achieve the effect of selecting a single channel.

[0063] The detector 5 can be selected from a spectrophotometer with a flow-through detection cell, a fluorescence photometer, an atomic absorption photometer, an inductively coupled plasma emission spectrometer, an inductively coupled plasma mass spectrometer, and an electrochemical detector, etc.

[0064] The five-way joint 6 is a connection module containing five connected interfaces, including a common interface 6-0 and a first interface 6-1, a second interface 6-2, a third interface 6-3, and a fourth interface 6-4 that are connected to the common interface 6-0. The five-way joint 6 can be fired from glass or quartz, or can be processed from inert plastics such as polytetrafluoroethylene, plexiglass, or polyether ether ketone.

[0065] The temperature-controlled reactor 7 is composed of a temperature-controllable heating rod and a polytetrafluoroethylene tube or a quartz tube wound around the heating rod, and there is a heat-insulating shell outside, and the temperature range that can be controlled is from room temperature to 250 °C. The polytetrafluoroethylene tube or the quartz tube also functions as a liquid storage coil and a mixing coil, with an inner diameter of 0.5 mm to 3 mm and a length of 0.05 m to 10 m.

[0066] The ultraviolet digester 8 is composed of an ultraviolet lamp tube and a quartz tube or a tube such as fluorinated ethylene propylene (FEP) that can transmit ultraviolet light wound around the lamp tube, with an inner diameter of 0.5 mm to 3 mm and a length of 0.05 m to 10 m. The ultraviolet lamp tube and the coil are encapsulated in a shell with a total internal reflection inner wall. The reaction coil can also function as a liquid storage coil and a mixing coil.

[0067] The pretreatment column 9 can be a solid-phase extraction column, an on-line oxidation or reduction column, etc. according to requirements.

[0068] The air filter 10 is a needle-type filter with a pore size of 0.1 μm to 0.45 μm, which is used to prevent particulate matter in the air from entering the analyzer.

[0069] The above components are connected through a polytetrafluoroethylene tube to form a liquid flow path. The inner diameter of the polytetrafluoroethylene tube is 0.5 mm to 3 mm, and the outer diameter is 1.6 mm to 5 mm.

[0070] As Figure 1 shown, the respective branch interfaces a, b / c / d / e / f, g, h, i, j, k, l surrounded on the multi-position selection valve 1 are respectively connected to the sample bottle 11, the reaction reagent bottles 12-1 / 12-2 / 12-3 / 12-4 / 12-5, the temperature-controlled reactor 7, the ultraviolet digestion device 8, the pretreatment column 9, the fourth interface 6-4 of the five-way joint, the inlet of the detector 5, and the cleaning liquid bottle 13. The common interface 1-0 of the multi-position selection valve 1 is connected to one interface of the two-way pump 2, and the other interface of the two-way pump 2 is connected to the bottom interface 3-1 of the mixing separator 3; the other interfaces of the temperature-controlled reactor 7, the ultraviolet digestion device 8, and the pretreatment column 9 are respectively connected to the first interface 6-1 of the five-way joint, the second interface 6-2 of the five-way joint, and the third interface 6-3 of the five-way joint; the common interface 6-0 of the five-way joint is connected to the common interface 4-0 of the three-way valve 4; the first interface 4-1 of the three-way valve 4 is connected to the waste liquid bottle 14, and the second interface 4-2 of the three-way valve 4 is connected to the upper-middle interface 3-3 of the mixing separator 3; the top interface 3-2 of the mixing separator 3 is connected to the air filter 10.

[0071] In use, the multi-position selection valve 1 is switched to the valve position a where the sample bottle 11 is located, and the bidirectional pump 2 is started and operated at a constant flow rate for a certain period of time to pump a certain amount of sample into the mixing separator 3, and then the bidirectional pump 2 is stopped; the multi-position selection valve 1 is successively switched to the valve positions b / c / d / e / f of the required reaction reagent bottles 12-1 / 12-2 / 12-3 / 12-4 / 12-5, the bidirectional pump 2 is started and operated at a constant flow rate for a certain period of time, and a certain amount of reagent is successively pumped in; at this time, depending on the requirements of the reaction conditions, if mixing reaction is required at a certain temperature or ultraviolet digestion is required or pretreatment column 9 is required, the multi-position selection valve 1 is switched to the valve position g or h or i, the three-way valve 4 is switched to the second interface 4-2, the bidirectional pump 2 is started, and the solution in the mixing separator 3 is pumped out at a constant flow rate, enters the temperature-controlled reactor 7 or ultraviolet digester 8 or pretreatment column 9 through the valve position g or h or i of the multi-position selection valve 1, then returns to the mixing separator 3 through the common interface 6-0 of the five-way valve 6, the common interface 4-0 of the three-way valve 4 and the second interface 4-2, forming a circulating mixing state; if no pretreatment is required for the reaction, only the multi-position selection valve 1 needs to be switched to the valve position j, the three-way valve 4 is switched to the second interface 4-2, the bidirectional pump 2 is started, and the solution in the mixing separator 3 returns to the mixing separator 3 through the valve position j of the multi-position selection valve 1, the fourth interface 6-4 and the common interface 6-0 of the five-way valve 6, the common interface 4-0 of the three-way valve 4 and the second interface 4-2, forming a circulating mixing state. After mixing for a certain period of time, if the reaction is completed, the bidirectional pump 2 is stopped, the multi-position selection valve 1 is switched to the valve position k, the bidirectional pump 2 is started, the solution in the mixing separator 3 is sent into the detector 5, the signal value is recorded, and then discharged into the waste liquid bottle 14; if the reaction is not completed and other reagents need to be added, the multi-position selection valve 1 is continuously switched to the valve position of the required reagent bottle, and the above processes of adding reagents and mixing are repeated until all the required reagents are added and all the mixing reactions are completed, and then the mixed solution is sent into the detector 5 for detection. After the detection is completed, the multi-position selection valve 1 switches the valve position to the valve position l of the cleaning solution bottle 13, the bidirectional pump 2 is started, the cleaning solution is pumped into and fills the mixing separator 3, and then the bidirectional pump 2 is stopped; the multi-position selection valve 1 is successively switched to each of the previously used pretreatment valve positions, and the bidirectional pump 2 is started to clean all the valve positions and flow path pipes.

[0072] The present invention will be further described below through experimental examples.

[0073] Experimental Example 1: Automatic Chemical Analysis Detection Device Using a Spectrophotometer and Determination of Nitrite Nitrogen in Seawater

[0074] Chemical reaction principle: Under acidic conditions, nitrite undergoes a diazotization reaction with sulfanilamide, and its product then couples with N-(1-Naphthyl)ethylenediamine dihydrochloride to form a red azo dye. The absorbance is measured at a wavelength of 543 nm, and the absorbance is proportional to the nitrite content.

[0075] Implementation steps: As Figure 1As shown, for the full-automatic chemical analysis and detection device of the embodiment, the detector 5 uses a spectrophotometer equipped with a flow cell with an optical path length of 3 cm, the detection wavelength is 543 nm, the pipelines connecting each component are polytetrafluoroethylene tubes with an inner diameter of 0.8 mm and an outer diameter of 1.6 mm, and all the reagents used are of analytical grade. The multi-position selection valve 1 used is a twelve-position valve, the cleaning solution is deionized water, the reaction reagent bottle 12-1 contains a sulfonamide solution with a concentration of 10 g / L and is prepared in 1.2 mol / L hydrochloric acid, and the reaction reagent bottle 12-2 contains a solution of N-(1-Naphthyl)ethylenediamine dihydrochloride with a concentration of 1 g / L. The internal tubular cavity of the mixing separator 3 used has a diameter of 10 mm, a height of 60 mm, and a conical height of 15 mm, and the entire cavity volume is about 5.5 mL; the distance between the middle-upper interface 3-3 and the top interface 3-2 of the mixing separator 3 is 2 mm. The two-way pump 2 used is a peristaltic pump and is equipped with a silicone rubber pump tube with an inner diameter of 0.89 mm. The three-way valve 4 used is an electromagnetic three-way valve. The temperature-controlled reactor 7, the ultraviolet digester 8, and the pretreatment column 9 are all installed normally, but during the detection process, they are not switched to the corresponding valve positions, and the power supplies of the temperature-controlled reactor 7 and the ultraviolet digester 8 are both disconnected.

[0076] The twelve-position selection valve 1 is switched to the valve position a of the sample bottle 11. After starting the two-way pump 2 and pumping 5 mL of the water sample into the mixing separator 3, the two-way pump 2 stops; the twelve-position selection valve 1 is switched to the valve position b of the reaction reagent bottle 12-1. After starting the two-way pump 2 and pumping 0.1 mL of the sulfonamide solution, the two-way pump 2 stops; the twelve-position selection valve 1 is switched to the valve position j, the three-way valve 4 is switched to the second interface 4-2, and the two-way pump 2 is started in the reverse direction to extract the solution from the bottom interface 3-1 of the mixing separator 3, and through the valve position j, the fourth interface 6-4 and the common interface 6-0 of the five-way valve 6, the common interface 4-0 and the second interface 4-2 of the three-way valve 4, it returns to the inside of the mixing separator 3 from the middle-upper interface 3-3 of the mixing separator 3 to form a circulating mixing state, and after continuous mixing for 1 min, it stops.

[0077] The twelve-position selection valve 1 is switched to the valve position c of the reaction reagent bottle 12-2. After starting the two-way pump 2 to pump in 0.1 mL of the N-(1-Naphthyl)ethylenediamine dihydrochloride solution, the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position j, and the two-way pump 2 is started in reverse to draw the solution from the bottom interface 3-1 of the mixing separator 3, and then back into the interior of the mixing separator 3 through the valve position j, the fourth interface 6-4 and the common interface 6-0 of the five-way joint 6, and the common interface 4-0 and the second interface 4-2 of the three-way valve 4 from the upper-middle interface 3-3 of the mixing separator 3, forming a circulating mixing state. After continuous mixing for 1 minute, it stops. The direction of the two-way pump 2 is switched and it runs for 10 s to draw the solution in the pipeline between the valve position j and the upper-middle interface 3-3 of the mixing separator 3 back into the mixing separator 3, and the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position k, the two-way pump 2 is started, the solution in the mixing separator 3 is sent to the detector 5 for detection. After recording the absorbance, it is discharged to the waste liquid bottle 14, and the two-way pump 2 stops.

[0078] The twelve-position selection valve 1 is switched to the valve position l of the cleaning solution bottle 13. After starting the two-way pump 2 to pump the cleaning solution into and fill the mixing separator 3, the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position j, and the two-way pump 2 is started in reverse. After using the cleaning solution in the mixing separator 3 to clean the pipeline between the valve position j and the mixing separator 3 for 30 s, the three-way valve 4 is switched to the first interface 4-1 to discharge the cleaning solution to the waste liquid bottle 14. The twelve-position selection valve 1 is switched to the valve position l of the cleaning solution, the two-way pump 2 is started to pump the cleaning solution into and fill the mixing separator 3, and then the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position k, and the two-way pump 2 is started in reverse to clean the detector 5 with the cleaning solution and empty the mixing separator 3.

[0079] Repeat the above steps to start the detection of the next sample.

[0080] In this experimental example, the linear equation of the standard curve of nitrite nitrogen in seawater is A = 2.0239C 亚硝氮 - 0.0076 (n = 7, R 2 = 0.9991), where A is the absorbance recorded by the spectrophotometer, and C 亚硝氮 is the concentration of nitrite nitrogen, with the unit of mg / L (calculated as nitrogen). The linear range is between 0.0075 mg / L and 0.6 mg / L. The measured concentration of nitrite in the surface seawater near Xiamen Baicheng is between 0.058 mg / L and 0.084 mg / L, and the spiked recovery rate is between 93.2% and 107.8%.

[0081] Experimental Example 2: Determination of total nitrogen in water using a fully automatic chemical analysis detection device of a spectrophotometer

[0082] Principle of chemical reaction: Under alkaline conditions, nitrogen-containing compounds in the sample are oxidized to nitrates by persulfate under ultraviolet irradiation at 95°C ± 2°C, and then reduced to nitrites by a cadmium column; nitrites undergo a diazotization reaction with sulfanilamide under acidic conditions, and the product then couples with N-(1-Naphthyl)ethylenediamine dihydrochloride to form a red azo dye, and the absorbance is measured at a wavelength of 543 nm.

[0083] Implementation steps: As Figure 1 shown, the full-automatic chemical analysis and detection device of the embodiment is adopted. The detector 5 uses a spectrophotometer equipped with a 1-cm optical path flow cell, the detection wavelength is 543 nm, the pipeline connecting each component is a polytetrafluoroethylene tube with an inner diameter of 0.8 mm and an outer diameter of 1.6 mm, and all reagents used are of analytical grade. The internal coil of the temperature-controlled reactor 7 adopted is a polytetrafluoroethylene tube with a length of 4 m, an inner diameter of 0.8 mm, and an outer diameter of 1.6 mm, and the temperature is set at 95°C ± 0.2°C; the ultraviolet lamp power of the ultraviolet digester 8 adopted is 14 W, and the internally wound coil is a fluorinated ethylene propylene (FEP) tube with a length of 4 m, an inner diameter of 0.8 mm, and an outer diameter of 1.6 mm; the pretreatment column 9 adopted is a cadmium reduction column, 60 mm long, with an inner diameter of 3.2 mm and an outer diameter of 5.3 mm; the cleaning solution is deionized water. The reaction reagent bottle 12-1 contains a potassium persulfate solution with a concentration of 50 g / L, the reaction reagent bottle 12-2 contains a sodium tetraborate buffer solution with a concentration of 25 g / L and a pH of 9.0, the reaction reagent bottle 12-3 contains an ammonium chloride buffer solution with a concentration of 85 g / L and a pH of 8.5, and the reaction reagent bottles 12-4 and 12-5 contain the same sulfanilamide solution and N-(1-Naphthyl)ethylenediamine dihydrochloride solution as in Experimental Example 1 respectively. The multi-position selection valve 1, the two-way pump 2, the mixing separator 3, and the three-way valve 4 are all the same as in Experimental Example 1.

[0084] The twelve-position selection valve 1 is switched to the valve position a of the sample bottle 11. The two-way pump 2 is started, and after pumping 1.6 mL of the water sample into the mixing separator 3, the two-way pump 2 stops; the twelve-position selection valve 1 is switched to the valve position b of the reaction reagent bottle 12-1, the two-way pump 2 is started, and after pumping 0.4 mL of the potassium persulfate solution, the two-way pump 2 stops; the twelve-position selection valve 1 is switched to the valve position g, the three-way valve 4 is switched to the second interface 4-2, the two-way pump 2 is started in reverse, the solution is drawn out from the bottom interface 3-1 of the mixing separator 3, passes through the valve position g, the temperature-controlled reactor 7, and the second interface 4-2 of the three-way valve 4, and returns to the inside of the mixing separator 3 from the upper-middle interface 3-3 of the mixing separator 3 to form a circulating mixing state, and the solution repeatedly passes through the temperature-controlled reactor 7 for high-temperature digestion; after the two-way pump 2 runs continuously for 2 min, it runs in the reverse direction for 30 s to draw the solution in the coil of the temperature-controlled reactor 7 back into the mixing separator 3, and the two-way pump 2 stops.

[0085] The twelve-position selection valve 1 is switched to the valve position c of the reaction reagent bottle 12-2. The two-way pump 2 is started. After pumping 0.4 mL of sodium tetraborate buffer solution, the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position h. The three-way valve 4 is switched to the second interface 4-2. The two-way pump 2 is started in the reverse direction. The solution is drawn out from the bottom interface 3-1 of the mixing separator 3, passes through the valve position h, the ultraviolet digestion device 8, and the second interface 4-2 of the three-way valve 4, and returns to the inside of the mixing separator 3 from the upper-middle interface 3-3 of the mixing separator 3 to form a circulating mixing state. The solution repeatedly passes through the ultraviolet digestion device 8 for digestion. After the two-way pump 2 runs continuously for 2 min, it runs in the reverse direction for 30 s to draw the solution in the coil of the ultraviolet digestion device 8 back into the mixing separator 3, and the two-way pump 2 stops. Thus, the nitrogen-containing compounds in the sample are oxidized to nitrates.

[0086] The twelve-position selection valve 1 is switched to the valve position d of the reaction reagent bottle 12-3. The two-way pump 2 is started. After pumping 2.4 mL of ammonium chloride buffer solution, the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position i. The three-way valve 4 is switched to the first interface 4-1. The two-way pump 2 is started in the reverse direction. The solution is drawn out from the bottom interface 3-1 of the mixing separator 3. The original ammonium chloride buffer solution in the cadmium reduction column 9 is discharged to the waste liquid bottle 14 through the valve position i and the first interface 4-1 of the three-way valve 4. After discharging 0.5 mL, the three-way valve 4 is switched to the second interface 4-2. The solution flows back into the mixing separator 3. The two-way pump 2 runs continuously for 1 min to make the mixed solution repeatedly flow through the cadmium reduction column 9 to reduce the nitrates in the sample to nitrites, and the two-way pump 2 stops.

[0087] The twelve-position selection valve 1 is switched to the valve position e of the reaction reagent bottle 12-4. The two-way pump 2 is started. After pumping 0.1 mL of sulfanilamide solution, the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position j. The three-way valve 4 is switched to the second interface 4-2. The two-way pump 2 is started in the reverse direction. The solution is drawn out from the bottom interface 3-1 of the mixing separator 3, passes through the valve position j, the fourth interface 6-4 and the common interface 6-0 of the five-way valve 6, the common interface 4-0 and the second interface 4-2 of the three-way valve 4, and returns to the inside of the mixing separator 3 from the upper-middle interface 3-3 of the mixing separator 3 to form a circulating mixing state. After the two-way pump 2 runs continuously for 1 min, it stops.

[0088] The twelve-position selection valve 1 is switched to the valve position f of the reaction reagent bottle 12-5. After starting the two-way pump 2 to pump in 0.1 mL of N-(1-Naphthyl)ethylenediamine dihydrochloride solution, the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position j, and the two-way pump 2 is started in reverse to extract the solution from the bottom interface 3-1 of the mixing separator 3, and then back into the interior of the mixing separator 3 through the valve position j, the fourth interface 6-4 and the common interface 6-0 of the five-way joint 6, and the common interface 4-0 and the second interface 4-2 of the three-way valve 4 from the upper-middle interface 3-3 of the mixing separator 3, forming a circulating mixing state. After the two-way pump 2 runs continuously for 1 min, it stops, changes direction and runs for 10 s to pump back the solution in the pipeline between the valve position j and the upper-middle interface 3-3 of the mixing separator 3 into the mixing separator 3, and then stops the two-way pump 2. The twelve-position selection valve 1 is switched to the valve position k, and the two-way pump 2 is started in reverse to send the solution in the mixing separator 3 into the detector 5 for detection. After recording the absorbance, it is discharged into the waste liquid bottle 14, and then the two-way pump 2 stops.

[0089] The twelve-position selection valve 1 is switched to the valve position l of the cleaning solution bottle 13. After starting the two-way pump 2 to pump the cleaning solution into and fill the mixing separator 3, the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position g, and the three-way valve 4 is switched to the first interface 4-1. The two-way pump 2 is started in reverse to clean the coil of the temperature-controlled reactor 7 with the cleaning solution in the mixing separator 3 and then drain it, and then the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position l of the cleaning solution bottle 13. After starting the two-way pump 2 to pump the cleaning solution into and fill the mixing separator 3, the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position h, and the three-way valve 4 is switched to the first interface 4-1. The two-way pump 2 is started in reverse to clean the coil of the ultraviolet digestion device 8 with the cleaning solution in the mixing separator 3 and then drain it, and then the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position l of the cleaning solution bottle 13. After starting the two-way pump 2 to pump the cleaning solution into and fill the mixing separator 3, the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position k, and the two-way pump 2 is started in reverse to clean the detector 5 with the cleaning solution and drain the mixing separator 3.

[0090] The twelve-position selection valve 1 is switched to the valve position d of the reaction reagent bottle 12-3. After starting the two-way pump 2 to pump 2.5 mL of ammonium chloride buffer solution into the mixing separator 3, the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position i, and the three-way valve 4 is switched to the first interface 4-1. The two-way pump 2 is started in reverse to send the ammonium chloride buffer solution into the cadmium reduction column 9, discharge the remaining solution in it, and fill the cadmium reduction column 9 with ammonium chloride solution.

[0091] The twelve-position selector valve 1 is switched to the valve position l for the cleaning liquid bottle 13, and the two-way pump 2 is started. After the cleaning liquid is pumped into and fills the mixing separator 3, the two-way pump 2 is stopped; the twelve-position selector valve 1 is switched to the valve position j, and the two-way pump 2 is started in reverse. After cleaning the pipeline between the valve position j, the five-way joint 6 and the three-way valve 4 with the cleaning liquid in the mixing separator 3 for 30 s, the three-way valve 4 is switched to the first interface 4-1, and the cleaning liquid is discharged to the waste liquid bottle 14 and the pipeline between the valve position j and the first interface 4-1 of the three-way valve 4 is emptied.

[0092] Repeat the above steps to start the detection of the next sample.

[0093] In this experimental example, the linear equation of the standard curve for the total nitrogen prepared with glycine in water is A = 0.2012C 总氮 + 0.0620 (n = 6, R 2 = 0.9997), where A is the absorbance recorded by the spectrophotometer, and C 总氮 is the concentration of total nitrogen, with the unit of mg / L (calculated as nitrogen), and the linear range is between 0.08 mg / L and 5 mg / L. The total nitrogen concentration in the Furong Lake water of Xiamen University was measured to be 1.83 mg / L, and the spike recovery rate was between 90.4% and 105.2%.

[0094] Experimental example 3: Determination of anionic surfactants in water using a fully automatic chemical analysis detection device of a spectrophotometer

[0095] Chemical reaction principle: Anionic surfactants form methylene blue active substances with the cationic dye methylene blue. After extraction with chloroform, the absorbance of the organic phase is measured at a wavelength of 650 nm, and the absorbance is proportional to the concentration of anionic surfactants.

[0096] Implementation steps: As Figure 1As shown in the figure, for the full-automatic chemical analysis and detection device of this embodiment, the detector 5 uses a spectrophotometer equipped with a 1-cm optical path flow cell, the detection wavelength is 650 nm, the pipeline connecting each component is a polytetrafluoroethylene tube with an inner diameter of 0.8 mm and an outer diameter of 1.6 mm, and all the reagents used are of analytical purity. The internal coil of the temperature-controlled reactor 7 used is a polytetrafluoroethylene tube with a length of 4 m, an inner diameter of 0.8 mm, and an outer diameter of 1.6 mm, and the temperature is set at room temperature; the ultraviolet digester 8 and the pretreatment column 9 are both installed normally, but the corresponding valve positions are not switched during the detection process, and the ultraviolet lamp power supply is disconnected; the cleaning solution is a 20% (v / v) isopropyl alcohol solution, the reaction reagent bottle 12-1 contains an alkaline methylene blue solution with a concentration of 0.02 g / L, the reaction reagent bottle 12-2 contains chloroform, the reaction reagent bottle 12-3 contains an acidic methylene blue solution with a concentration of 0.02 g / L, and the reaction reagent bottles 12-4 and 12-5 are not used in this embodiment. Except that the pump tube is changed to a fluororubber tube with an inner diameter of 0.76 mm, the twelve-position selection valve 1, the two-way pump 2, the mixing separator 3, and the three-way valve 4 are all the same as in Experimental Example 1.

[0097] When measuring the sample, the twelve-position selection valve 1 is switched to the valve position a of the sample bottle 11, the two-way pump is started, and after pumping 3.2 mL of the water sample into the mixing separator 3, the two-way pump 2 stops; the twelve-position selection valve 1 is switched to the valve position b of the reaction reagent bottle 12-1, the two-way pump 2 is started, and after pumping 1 mL of the alkaline methylene blue solution, the two-way pump 2 stops; the twelve-position selection valve 1 is switched to the valve position j, the three-way valve 4 is switched to the second interface 4-2, the two-way pump 2 is started in the reverse direction, the solution is drawn out from the bottom interface 3-1 of the mixing separator 3, and passes through the valve position j, the fourth interface 6-4 and the common interface 6-0 of the five-way joint 6, the common interface 4-0 and the second interface 4-2 of the three-way valve 4, and returns to the inside of the mixing separator 3 from the middle-upper interface 3-3 of the mixing separator 3 to form a circulating mixing state. The anionic surfactant in the sample reacts with methylene blue to form methylene blue active substance. After continuous mixing for 1 min, the two-way pump 2 stops.

[0098] The twelve-position selection valve 1 is switched to the valve position c of the reaction reagent bottle 12-2. After starting the bi-directional pump 2 and pumping 1 mL of chloroform, the bi-directional pump 2 stops. The twelve-position selection valve 1 is switched to the valve position j, and the three-way valve 4 is switched to the second interface 4-2. The bi-directional pump 2 is started in the reverse direction to extract the solution from the bottom interface 3-1 of the mixing separator 3, and it returns to the inside of the mixing separator 3 from the upper-middle interface 3-3 of the mixing separator 3 through the valve position j, the fourth interface 6-4 and the common interface 6-0 of the five-way valve 6, and the common interface 4-0 and the second interface 4-2 of the three-way valve 4, forming a circulating mixing state. The chloroform is fully mixed with the sample, and the methylene blue active substance is extracted into the chloroform. After continuously mixing and extracting for 3 min, the bi-directional pump 2 runs in the reverse direction for 30 s to pump the solution in the pipeline back into the mixing separator 3, and then the bi-directional pump 2 stops. The solution is left standing in the mixing separator 3 for 1 min. At this time, the chloroform and the aqueous phase are separated under the action of gravity and remain at the bottom of the mixing separator 3.

[0099] The twelve-position selection valve 1 is switched to the valve position g. After starting the bi-directional pump 2, 1.2 mL of the liquid at the bottom of the mixing separator 2 is extracted and sent to the coil of the temperature-controlled reactor 7 for storage. To ensure that all the chloroform is extracted, the volume of the liquid extracted here is greater than the volume of the added chloroform. The twelve-position selection valve 1 is switched to the valve position j, and the three-way valve 4 is switched to the first interface 4-1. After starting the bi-directional pump 2, the remaining solution in the mixing separator 3 is extracted from the bottom interface 3-1 and discharged to the waste liquid bottle 14 through the valve position j, the fourth interface 6-4 and the common interface 6-0 of the five-way valve 6, and the common interface 4-0 and the first interface 4-1 of the three-way valve 4.

[0100] The twelve-position selection valve 1 is switched to the valve position d of the reaction reagent bottle 12-3. The two-way pump 2 is started, and after 1.2 mL of acidic methylene blue solution is pumped in, the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position g, the two-way pump 2 is started, and the solution in the coil of the temperature-controlled reactor 7 is pumped back to the mixing separator 3 to be mixed with the acidic methylene blue solution. After the solution in the coil is emptied, the two-way pump 2 stops. The twelve-position selection valve 1 is switched to the valve position j, the three-way valve 4 is switched to the second interface 4-2, the two-way pump 2 is started in the reverse direction, and the solution is drawn out from the bottom interface 3-1 of the mixing separator 3, passed through the valve position j, the fourth interface 6-4 and the common interface 6-0 of the five-way valve 6, the common interface 4-0 and the second interface 4-2 of the three-way valve 4, and returned to the inside of the mixing separator 3 from the upper-middle interface 3-3 of the mixing separator 3 to form a circulating mixing state, and the interfering substances in the chloroform are back-extracted into the acidic methylene blue solution; after continuous mixing and extraction for 3 min, the two-way pump 2 runs in the reverse direction for 30 s to pump the solution in the pipeline back into the mixing separator 3, and the two-way pump 2 stops, and the solution is allowed to stand in the mixing separator 3 for 1 min. At this time, the chloroform and the aqueous phase are separated under the action of gravity and remain at the bottom of the mixing separator 3. The twelve-position selection valve 1 is switched to the valve position k, the two-way pump 2 is started in the reverse direction, 0.8 mL of the chloroform at the bottom of the mixing separator 3 is sent to the detector 5 for detection. After the absorbance is recorded, it is discharged to the waste liquid bottle 14, and the two-way pump 2 stops.

[0101] The twelve-position selector valve 1 is switched to valve position j, the three-way valve 4 is switched to the first interface 4-1, the bidirectional pump 3 is started, and the remaining solution in the mixing separator 3 is pumped out from the bottom interface 3-1, passing through valve position j, the fourth interface 6-4 and the common interface 6-0 of the five-way valve 6, the common interface 4-0 and the first interface 4-1 of the three-way valve 4, and discharged into the waste liquid bottle 14. The twelve-position selector valve 1 is switched to valve position l of the cleaning solution bottle 13, the bidirectional pump 2 is started, the cleaning solution is pumped into and fills the mixing separator 3, and then the bidirectional pump 2 is stopped; the twelve-position selector valve 1 is switched to valve position g, the three-way valve 4 is switched to the first interface 4-1, the bidirectional pump 2 is started in the reverse direction, the coil of the temperature-controlled reactor 7 is cleaned with the cleaning solution in the mixing separator 3 and then emptied, and then the bidirectional pump 2 is stopped. The twelve-position selector valve 1 is switched to valve position c of the reaction reagent bottle 12-2, the bidirectional pump 2 is started, 3 mL of chloroform is pumped in, and then the bidirectional pump 2 is stopped; the twelve-position selector valve 1 is switched to valve position k, the bidirectional pump 2 is started in the reverse direction, the detector 5 is cleaned with 2.5 mL of chloroform, and then the bidirectional pump 2 is stopped; the twelve-position selector valve 1 is switched to valve position j, the three-way valve 4 is switched to the first interface 4-1, the bidirectional pump 2 is started, and the remaining chloroform is discharged through valve position j, the fourth interface 6-4 and the common interface 6-0 of the five-way valve 6, the common interface 4-0 and the first interface 4-1 of the three-way valve 4, into the waste liquid bottle 14. The twelve-position selector valve 1 is switched to valve position l of the cleaning solution bottle 13, the bidirectional pump 2 is started, the cleaning solution is pumped into and fills the mixing separator 3, and then the bidirectional pump 2 is stopped; the twelve-position selector valve 1 is switched to valve position j, the three-way valve 4 is switched to the second interface 4-2, the bidirectional pump 2 is started in the reverse direction, the pipeline between valve position j, the five-way valve 6 and the three-way valve 4 is cleaned with the cleaning solution in the mixing separator 3 for 30 s, then the three-way valve 4 is switched to the first interface 4-1, the cleaning solution is discharged into the waste liquid bottle 14 and the pipeline between valve position j and the first interface 4-1 of the three-way valve 4 is emptied.

[0102] Subsequently, repeat the above steps to start the detection of the next sample.

[0103] In this experimental example, the linear equation of the standard curve for the anion surfactant prepared with sodium dodecylbenzenesulfonate in water is A = 0.1536C 阴离子 + 0.0006 (n = 6, R 2 = 0.9995), where A is the absorbance recorded by the spectrophotometer, and C 阴离子 is the concentration of the anion surfactant, with the unit of mg / L (calculated as sodium dodecylbenzenesulfonate), and the linear range is between 0.040 mg / L and 2.0 mg / L. The concentration of the anion surfactant in the Furong Lake water of Xiamen University was measured to be 0.073 mg / L, and the spiked recovery rate was between 92.2% and 115.6%.

[0104] The above is only a preferred embodiment of the present invention, and thus the scope of implementation of the present invention cannot be limited thereby. That is, equivalent changes and modifications made according to the scope of the present invention patent and the content of the specification should still fall within the scope covered by the present invention.

Claims

1. A fully automatic chemical analysis and detection device, characterized in that: It includes: a sampling module, a pretreatment module, a mixing and separation module, a detection module, a two-way power module, a multi-channel connection module, a three-way connection module, a five-way connection module, and pipelines connecting each module; among them, the sampling module includes a number of reagent bottles arranged in parallel, and the number of reagent bottles are respectively filled with samples, reaction reagents, and cleaning solutions; the pretreatment module includes a temperature-controlled reactor, an ultraviolet digester, and a pretreatment column arranged in parallel; the mixing and separation module includes a mixing separator; the mixing separator has a spindle-shaped cavity inside, and the mixing separator is provided with a bottom interface, a top interface, and a middle-upper interface communicating with the cavity; the detection module includes a detector and a waste liquid bottle arranged in series; the two-way power module communicates with one end of the multi-channel connection module and the bottom interface of the mixing separator; one end of the multi-channel connection module communicates with the two-way power module, and the other end of the multi-channel connection module selectively communicates with the number of reagent bottles, the temperature-controlled reactor, the ultraviolet digester, the pretreatment column, the five-way connection module, and the detection module; one end of the three-way connection module communicates with the five-way connection module, and the other end of the three-way connection module selectively communicates with the waste liquid bottle of the detection module and the middle-upper interface of the mixing separator; one end of the five-way connection module communicates with the three-way connection module, and the other end of the five-way connection module selectively communicates with the temperature-controlled reactor, the ultraviolet digester, the pretreatment column, and the multi-channel connection module; the multi-channel connection module is a multi-position selection valve, and the common interface of the multi-position selection valve selectively communicates with a number of branch interfaces of the multi-position selection valve; the common interface of the multi-position selection valve communicates with the two-way power module, and the number of branch interfaces respectively communicate with the number of reagent bottles, the temperature-controlled reactor, the ultraviolet digester, the pretreatment column, the five-way connection module, and the detection module; the three-way connection module is a three-way valve, and the common interface of the three-way valve selectively communicates with the first interface of the three-way valve and the second interface of the three-way valve; the common interface of the three-way valve communicates with the five-way connection module, the first interface of the three-way valve communicates with the waste liquid bottle, and the second interface of the three-way valve communicates with the middle-upper interface of the mixing separator; the five-way connection module is a five-way, and the common interface of the five-way communicates with the three-way connection module, the first interface of the five-way communicates with the temperature-controlled reactor, the second interface of the five-way communicates with the ultraviolet digester, the third interface of the five-way communicates with the pretreatment column, and the fourth interface of the five-way communicates with the multi-channel connection module; the two-way power module includes a two-way pump, and the two-way pump is a peristaltic pump or a multi-plunger pump with a positive and reverse rotation function.

2. The fully automatic chemical analysis and detection device according to claim 1, characterized in that: the multi-position selection valve is an eight-bit, ten-bit, twelve-bit, fourteen-bit or sixteen-bit selection valve.

3. The fully automatic chemical analysis and detection device according to claim 1, characterized in that: The multi-channel connection module is several switching valves or several three-way valves arranged in parallel; the three-way connection module is two switching valves arranged in parallel.

4. The fully automatic chemical analysis and detection device according to claim 1, characterized in that: The middle section of the cavity of the mixing and separating device is tubular, with a diameter of 3 mm to 30 mm and a height of 3 mm to 70 mm. The two ends of the cavity are conical, with a height of 3 mm to 50 mm; the distance between the upper middle interface and the top interface is 10 mm.

5. The fully automatic chemical analysis and detection device according to claim 1, characterized in that: The mixing and separation module further includes an air filter, and the air filter communicates with the top interface of the mixing and separating device.

6. The fully automatic chemical analysis and detection device according to claim 1, characterized in that: The detector includes a spectrophotometer, a fluorescence photometer, an atomic absorption photometer, an inductively coupled plasma emission spectrometer, an inductively coupled plasma mass spectrometer or an electrochemical detector with a flow-through detection cell; the pretreatment column includes a solid phase extraction column, an on-line oxidation or reduction column.

7. A detection method using the fully automatic chemical analysis and detection device according to any one of claims 1 to 6, characterized in that: including: 1) The multi-channel connection module is switched to the reagent bottle containing the sample, and the sample is sent into the mixing and separating device through the bidirectional power module; Subsequently, step 2) is carried out, or step 3) is directly carried out; 2) The multi-channel connection module is switched to the pretreatment module, and the liquid in the mixing and separating device is sent into the pretreatment module through the bidirectional pump for pretreatment; after the pretreatment is completed, the liquid is sent back to the mixing and separating device through the bidirectional pump; subsequently, step 3) is carried out, or step 4) is directly carried out; 3) The multi-channel connection module is sequentially switched to each reagent bottle, and each reaction reagent is sequentially sent into the mixing and separating device through the bidirectional power module; subsequently, step 2) is repeated and then step 4) is carried out, or step 4) is directly carried out; 4) Cyclic mixing reaction; subsequently, step 2) or 3) is repeated, or step 5) is directly carried out; 5) The reaction solution is sent into the detector for detection through the bidirectional power module; 6) After the detection is completed, the multi-channel connection module is switched to the reagent bottle containing the cleaning solution, and the cleaning solution is sent into the mixing and separating device through the bidirectional power module; subsequently, the multi-channel connection module is sequentially switched to each module, and the cleaning solution in the mixing and separating device is sequentially sent into each module for cleaning.

Images