A gut bacterial micro-collector for insects

Abstract

The present application relates to the technical field of intestinal microorganism, and specifically relates to a kind of intestinal bacterial microorganism collector for insects, including collection forceps and the forceps head disinfection box matched with collection forceps;Forceps head disinfection box is used to store disposable forceps head, and disposable forceps head includes sharp head and hollow sharp body;Collection forceps include symmetrically arranged forceps handle;Forceps handle includes connecting head and hollow forceps rod, and tail end of forceps rod is fixedly connected with connecting head, and connecting head is detachably connected with sharp body;Control member for separating sharp body and connecting head is arranged on forceps rod.The present application designs both forceps and forceps head, and through disposable forceps head convenient to use not only realizes simple operation, improves experimental efficiency, but also only needs to replace disposable forceps head, effectively reduces the cost of experimental waste;In addition, forceps head disinfection box is convenient for alcohol disinfection to disposable forceps head, effectively avoids the problem of cross contamination of flora in experimental process.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of intestinal microorganisms, in particular to an intestinal bacterial microorganism collector for insects. BACKGROUND

[0002] Insects are the most abundant and largest class of animals in nature, with more than one million known species, which can survive and reproduce in various ecological environments and quickly adapt to changing environmental conditions, playing an important role in the ecological system. The exoskeleton, intestine, hemocoel and even cells of insects are distributed with many microorganisms. According to statistics, the total amount of microorganisms can account for 1-10% of the biomass of insects, which are an important part of the body of insects, and the species and quantity of intestinal microorganisms are the most; in recent years, it has been found that intestinal symbiotic microorganisms play an important role in the process of food digestion and nutrient utilization, growth and development, reproductive regulation and resistance to pathogens and harmful substances, so isolating and culturing or high-throughput sequencing of intestinal microorganisms of insects is the basis for discovering new species, new genes and new functions of microorganisms.

[0003] In the prior art, the intestinal bacterial microorganism of insects is often collected by using tweezers, the tip of the tweezers is used to clamp the tail of the insect, then a pulling force is applied to the tweezers, the intestinal tract of the insect is pulled out of the insect body by the action force of the tweezers, so that the intestinal tract is separated from the body, then the intestinal tract is ground and cultured in a reagent box or subjected to high-throughput sequencing to obtain intestinal bacterial microorganisms of different species of insects.

[0004] In order to realize more efficient separation of the intestinal tract, the tweezers used in the prior art are often precision tweezers, which are also commonly used for microscopic dissection of cells in the laboratory and are relatively expensive. In order to avoid cross contamination of bacterial flora, the tip of the tweezers needs to be sterilized after each intestinal separation experiment, specifically: the tip of the tweezers is first cleaned by spraying alcohol, and then sterilized by burning. With the increase of the number of times of burning, the tip of the tweezers gradually deforms. The deformed tip not only affects the operation of the intestinal separation experiment, but also easily breaks the intestinal tract during the experiment, resulting in sample waste. In addition, the deformed tweezers cannot be used again, so a new tweezers needs to be replaced, causing waste of experimental cost. SUMMARY

[0005] In view of the deficiencies in the prior art, the present application provides an intestinal bacterial microorganism collector for insects. The tweezers and the tweezers head are designed. The disposable tweezers head is convenient to use, which not only realizes simple operation and improves experimental efficiency, but also only needs to be replaced once, effectively reducing the cost of experimental waste. In addition, the tweezers head sterilization box is convenient for alcohol disinfection of the disposable tweezers head, effectively avoiding the problem of cross contamination of bacterial flora during the experiment.

[0006] To solve the above technical problems, the present application adopts the following technical solutions:

[0007] A kind of intestinal bacterial microorganism collector for insects, including collection forceps and the forceps head disinfection box matched with the collection forceps;

[0008] The forceps head disinfection box is used to store disposable forceps heads, which include a sharp head and a hollow sharp body, and the tip of the sharp body is fixed to the sharp head;

[0009] The collection forceps include two symmetrically arranged forceps handles, and the head ends of the two forceps handles are fixedly connected;The forceps handle includes a connecting head and a hollow forceps rod, and the tail end of the forceps rod is fixedly connected to the connecting head, and the connecting head is detachably connected to the sharp body;

[0010] A control member is arranged on the forceps rod for separating the sharp body and the connecting head, which includes a grip, a spring and a sleeve arranged on the forceps rod in sequence from top to bottom, a control rod and a crossbar arranged in the forceps rod, and a push head arranged on the connecting head and having a top wall fixedly connected to the tail end of the sleeve, wherein the tail end of the push head has a diameter less than or equal to the opening diameter of the sharp body;

[0011] The crossbar penetrates the side wall of the forceps rod and slides along the extension direction of the forceps rod, and the bottom end of the crossbar is fixedly connected to the top wall of the sleeve;

[0012] The bottom end of the control rod is fixedly connected to the crossbar, and the top end of the control rod is connected to a control panel, and one end of the control panel penetrates the side wall of the forceps rod and extends outward;

[0013] The grip is fixedly connected to the side wall of the forceps rod, the spring is arranged between the grip and the crossbar, and is fixedly connected to both of them.

[0014] Preferably, the forceps head disinfection box includes a box cover and a box body hingedly connected to each other, and a disinfection member for sterilizing the disposable forceps head is further arranged on the forceps head disinfection box, wherein the disinfection member includes:

[0015] An alcohol box is arranged through the outer wall of the forceps head disinfection box;

[0016] An ultrasonic atomization sheet is arranged at the communication position between the alcohol box and the forceps head disinfection box;

[0017] A fan is arranged at the bottom wall of the forceps head disinfection box;

[0018] An exhaust pipe is arranged through the side wall of the forceps head disinfection box, and a disposable bacterial filter membrane is detachably connected to the exhaust pipe;

[0019] A control switch is arranged at the connecting position between the box body and the box cover.

[0020] Preferably, the box is provided with a support, a plurality of limiting holes are arranged side by side on the support, and each limiting hole is clamped with the disposable forceps head.

[0021] Preferably, the connecting head is further provided with a clamping piece for stable connection, the clamping piece is a triangular clamping block, and the inner wall of the sharp body is further provided with a clamping groove corresponding in size to the triangular clamping block.

[0022] Preferably, a first sliding groove is arranged on the side wall of the forceps rod in the length direction of the forceps rod, one end of the control plate is fixedly connected with the control rod, and the other end penetrates through the first sliding groove and extends outward.

[0023] Preferably, a second sliding groove is arranged on the side wall of the forceps rod in the length direction of the forceps rod, and the two ends of the cross rod penetrate through two second sliding grooves respectively and slide in the second sliding grooves.

[0024] Preferably, the handle is provided with anti-skid lines.

[0025] Compared with the prior art, the present application has the beneficial effects that:

[0026] 1. The present application is provided with a collection forceps, two forceps handles of the collection forceps jointly act on the intestinal tract of the collected insect, the forceps handle comprises a connecting head and a forceps rod, the forceps rod is provided with a control piece for separating the sharp body and the connecting head, at this time, after the control plate is pressed, the control plate drives the control rod, the control rod drives the cross rod, the cross rod drives the sleeve, the sleeve drives the push head to move downward, after the push head moves downward, the bottom end of the push head first abuts against the top end of the sharp body, then the push head pushes the sharp body off the connecting head after an acting force is provided in the sharp head direction, at this time, the spring is in a stretched state, and after the hand releases the control plate, the spring returns to the original position, at this time, the spring drives the cross rod, the cross rod drives the sleeve, and the sleeve drives the push head to move upward and restore to the original position, so that the disposable forceps head can be replaced only by operating the control plate.

[0027] 2. The present application is provided with a forceps head disinfection box, the forceps head disinfection box is provided with a disposable forceps head, the forceps body of the disposable forceps head is detachably connected with the connecting head, the disposable forceps head is convenient to replace and use; in addition, the forceps head disinfection box is provided with a disinfection piece for conveniently performing alcohol disinfection on the disposable forceps head, and cross contamination of bacteria groups in the experimental process is effectively avoided. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 It is an internal structure sectional view of the forceps rod of the present application;

[0029] Figure 2 It is an internal structure schematic view of the forceps rod and the disposable forceps head after being connected of the present application;

[0030] Figure 3 Figure 6 is a cross-sectional view of the internal structure of a sterilization box for a tweezers head of a gut bacterial microorganism collector for insects according to the present application;

[0031] Figure 4 Figure 7 is a schematic view of the internal structure of a disposable tweezers head of a gut bacterial microorganism collector for insects according to the present application;

[0032] Figure 5 Figure 8 is a schematic view of the structure of a collection tweezers of a gut bacterial microorganism collector for insects according to the present application;

[0033] Figure 6 Figure 9 is a side view of a tweezers rod of a gut bacterial microorganism collector for insects according to the present application.

[0034] BRIEF DESCRIPTION OF THE DRAWINGS

[0035] 1. collection tweezers; 2. tweezers head sterilization box; 3. box cover; 4. disposable tweezers head; 5. pointed head; 6. pointed body; 7. box body; 8. tweezers handle; 9. connecting head; 10. tweezers rod; 11. handle; 12. spring; 13. sleeve; 14. control rod; 15. crossbar; 16. push head; 17. control board; 18. alcohol box; 19. ultrasonic atomization sheet; 20. fan; 21. discharge pipe; 22. disposable bacterial filter membrane; 23. control switch; 24. support; 25. triangular clamping block; 26. first sliding groove; 27. second sliding groove. DETAILED DESCRIPTION

[0036] The specific embodiments of the present application are described in detail below, but it should be understood that the scope of protection of the present application is not limited by the specific embodiments. Based on the examples in the present application, all other examples obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application. The experimental methods described in the embodiments of the present application are conventional methods unless otherwise specified.

[0037] In the description of the present application, it should be understood that the terms "length", "width", "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inner", "outer", etc. indicate the orientation or positional relationship based on the drawings shown in the figures, and are only used to facilitate the description of the technical solutions of the present application and simplify the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. Figure 1

[0038] Example 1

[0039] As Figure 5 and Figure 3 ​As shown in the figure, a kind of intestinal bacterial microorganism collector for insects includes collection forceps 1 and forceps head disinfection box 2 matched with the collection forceps 1, the collection forceps 1 is used to collect insect intestinal tract, and the forceps head disinfection box 2 is used to store disposable forceps head 4 and sterilize the disposable forceps head 4;

[0040] As shown in the figure, Figure 4 The disposable forceps head 4 includes a sharp head 5 and a hollow sharp body 6, and the tip of the sharp body 6 is fixedly connected with the sharp head 5;

[0041] As shown in the figure, Figure 2 The collection forceps 1 includes symmetrically arranged forceps handles 8, and the head ends of the two forceps handles 8 are fixedly connected; the forceps handle 8 includes a connecting head 9 and a hollow forceps rod 10, the tail end of the forceps rod 10 is fixedly connected with the connecting head 9, and the connecting head 9 is detachably connected with the sharp body 6;

[0042] As shown in the figure, Figure 1 The sharp body 6 and the connecting head 9 are separated by a control member arranged on the forceps rod 10, the control member includes a grip 11, a spring 12 and a sleeve 13 arranged on the forceps rod 10 in sequence from top to bottom, a control rod 14 and a crossbar 15 arranged in the forceps rod 10, and a push head 16 arranged on the connecting head 9 and having a top wall fixedly connected with the tail end of the sleeve 13, and the tail end diameter of the push head 16 is less than or equal to the opening diameter of the sharp body 6, so that the push head 16 is convenient for separating the sharp body 6 from the connecting head 9;

[0043] The two ends of the crossbar 15 penetrate the side wall of the forceps rod 10 and slide along the extension direction of the forceps rod 10, and the bottom end of the crossbar 15 is fixedly connected with the top wall of the sleeve 13, so that the crossbar 10 is convenient for controlling the sleeve 13 to slide, so that the sleeve 13 drives the push head 16 to slide; the second sliding grooves 27 are symmetrically arranged on the side wall of the forceps rod 10, the two ends of the crossbar 15 penetrate the two second sliding grooves 27 respectively and slide in the second sliding grooves 27, so that the crossbar 15 can slide in the second sliding grooves 27 and is convenient for driving the sleeve 13 and the push head 16 to move up and down;

[0044] The bottom end of the control rod 14 is fixedly connected with the crossbar 15, and the top end is connected with a control plate 17, one end of the control plate 17 penetrates the side wall of the forceps rod 10 and extends outward, after applying force to the control plate 17, the control plate 17 drives the control rod 14, the control rod 14 drives the crossbar 15, the crossbar 15 drives the sleeve 13 and the push head 16 to move; as shown in the figure, Figure 6 The first sliding groove 26 is arranged on the side wall of the forceps rod 10, one end of the control plate 17 is fixedly connected with the control rod 14, and the other end penetrates the first sliding groove 26 and extends outward, when the control plate 17 is pressed downward, the first sliding groove 26 is convenient for the control plate 17 to slide downward;

[0045] The handle 11 is fixed with the side wall of the tweezers rod 10, and the handle 11 is used for the experimenter to hold, facilitating experimental operation. The handle 11 is provided with anti-skid lines, and a certain friction force is generated between the anti-skid lines and the hand, so that the experimental operation hand is prevented from slipping off. The spring 12 is arranged between the handle 11 and the horizontal rod 15 and is fixed with the two, respectively. After an acting force is applied to the control plate 17, the push head 16 moves in the direction of the connecting head 9. After the sharp body 6 is separated from the connecting head 9, the spring is in a stretched state. After the hand releases the control plate 17, the spring 12 returns to the original position. At this time, the spring 12 drives the horizontal rod 15, the horizontal rod 15 drives the sleeve pipe 13, the sleeve pipe 13 drives the push head 16 to move upwards and restore to the original position.

[0046] Principle: in use, open the tweezers head disinfection box 2, pinch the handle 11 with fingers, connect the open end of the sharp body 6 to the two connecting heads 9, respectively, at this time, use the sharp head 5 to clamp the tail of the insect, then apply a pulling force to the tweezers, the intestinal tract of the insect is pulled out of the insect body by the action force of the collection tweezers 1, and then the intestinal tract is placed in a mortar for grinding; if it is necessary to further carry out an intestinal tract in vitro operation experiment, an acting force is applied to the control plate 17, at this time, the control plate 17 drives the control rod 14, the control rod 14 drives the horizontal rod 15, the horizontal rod 15 drives the sleeve pipe 13, the sleeve pipe 13 drives the push head 16 to slide, the push head 16 moves in the direction of the connecting head 9, after the sharp body 6 is separated from the connecting head 9, the spring is in a stretched state, and after the hand releases the control plate 17, the spring 12 returns to the original position, at this time, the spring 12 drives the horizontal rod 15, the horizontal rod 15 drives the sleeve pipe 13, the sleeve pipe 13 drives the push head 16 to move upwards and restore to the original position; the above operation is repeated.

[0047] Example 2

[0048] On the basis of example 1, in order to avoid the pollution of the disposable tweezers head 4, the tweezers head disinfection box 2 comprises a box cover 3 and a box body 7 which are hingedly connected, and the tweezers head disinfection box 2 is further provided with a disinfection device for sterilizing the disposable tweezers head 4. The disinfection device comprises:

[0049] An alcohol box 18 is arranged through the outer wall of the tweezers head disinfection box 2, and the alcohol box 18 stores alcohol used for sterilization;

[0050] An ultrasonic atomization sheet 19 is arranged at the communication position of the alcohol box 18 and the tweezers head disinfection box 2, so as to facilitate the atomization of alcohol, and the atomized alcohol is convenient for the overall disinfection of the disposable tweezers head 4;

[0051] A fan 20 is arranged at the bottom wall of the tweezers head disinfection box 2, and the fan 20 makes the alcohol evaporate as soon as possible;

[0052] The pipe 21 is arranged through the side wall of the sterilization box 2, and the disposable bacterial filter 22 is detachably connected to the pipe 21. The pipe 21 is in sealing connection with the disposable bacterial filter 22, the alcohol is discharged through the pipe 21, and the disposable bacterial filter 22 prevents the bacteria in the outside from entering the sterilization box 2 through the pipe 21, so that the disposable forceps 4 are not polluted.

[0053] The control switch 23 is arranged at the connecting position of the box body 7 and the box cover 3. When the box cover 3 is opened, the control switch 23 is closed, and the alcohol is stopped from being atomized. When the box cover 3 is closed, the control switch 23 is opened, so that the ultrasonic atomization piece 19 and the fan 20 are operated. The ultrasonic atomization piece 19, the fan 20 and the battery are in electrical connection with the control switch 23.

[0054] The bracket 24 is arranged in the box body 7, and a plurality of limiting holes are arranged side by side on the bracket 24. The disposable forceps 4 are clamped in the limiting holes, and the tips 5 of the disposable forceps 4 are arranged side by side. The bracket 24 facilitates the experimental operation and the sterilization of the tips 5.

[0055] Embodiment 3

[0056] In order to stably connect the connecting head 9 and the sharp body 6 and improve the experimental efficiency, the connecting head 9 is further provided with a clamping piece for stable connection. The clamping piece is a triangular clamping block 25, and the inner wall of the sharp body 6 is further provided with a clamping groove corresponding in size to the triangular clamping block 25. When the triangular clamping block 25 is connected to the triangular clamping block 25, the triangular clamping block 25 is clamped in the clamping groove corresponding in size, so as to facilitate the connection of the connecting head 9 and the sharp body 6 and prevent the disposable forceps 4 from falling during the experimental operation.

[0057] Obviously, those skilled in the art can make various modifications and variations to the present application without departing from the spirit and scope of the present application. Thus, if these modifications and variations of the present application belong to the scope of the claims of the present application and their equivalent technologies, the present application also intends to include these modifications and variations.

Claims

1. An enteral bacterial microorganism trap for insects, characterized in that, The application relates to a collection forceps (1) and a forceps head disinfection box (2) matched with the collection forceps (1). The forceps head disinfection box (2) is used for storing disposable forceps heads (4), the disposable forceps heads (4) comprise sharp heads (5) and hollow sharp bodies (6), and the sharp ends of the sharp bodies (6) are fixedly connected with the sharp heads (5). The collection forceps (1) comprise symmetrically arranged forceps handles (8), the head ends of the two forceps handles (8) are fixedly connected, the forceps handle (8) comprises a connecting head (9) and a hollow forceps rod (10), the tail end of the forceps rod (10) is fixedly connected with the connecting head (9), and the connecting head (9) is detachably connected with the sharp body (6). A control member for separating the sharp body (6) and the connecting head (9) is arranged on the forceps rod (10), the control member comprises, from top to bottom, a handle (11) sleeved on the forceps rod (10), a spring (12) and a sleeve (13), a control rod (14) and a cross rod (15) arranged in the forceps rod (10), and a push head (16) sleeved on the connecting head (9) and fixedly connected with the tail end of the sleeve (13) through a top wall, the tail end diameter of the push head (16) is less than or equal to the opening diameter of the sharp body (6), and the push head (16) can push the sharp body (6) off the connecting head (9). The two ends of the cross rod (15) penetrate through the side wall of the forceps rod (10) and can slide along the extension direction of the forceps rod (10), and the bottom end of the cross rod (15) is fixedly connected with the top wall of the sleeve (13). The bottom end of the control rod (14) is fixedly connected with the cross rod (15), and a control plate (17) is connected to the top end of the control rod (14), one end of the control plate (17) penetrates through the side wall of the forceps rod (10) and extends outward. The handle (11) is fixedly connected with the side wall of the forceps rod (10), the spring (12) is arranged between the handle (11) and the cross rod (15) and is fixedly connected with both.

2. The gut bacterial microorganism trap for insects according to claim 1, wherein, The forceps head disinfection box (2) comprises a box cover (3) and a box body (7) hingedly connected with each other, and a disinfection member for sterilizing the disposable forceps heads (4) is further arranged on the forceps head disinfection box (2), the disinfection member comprises: An alcohol box (18) is arranged through the outer wall of the forceps head disinfection box (2); An ultrasonic atomization sheet (19) is arranged at the communication position between the alcohol box (18) and the forceps head disinfection box (2); A fan (20) is arranged on the bottom wall of the forceps head disinfection box (2); A discharge pipe (21) is arranged through the side wall of the forceps head disinfection box (2), and a disposable bacterial filter membrane (22) is detachably connected to the discharge pipe (21); A control switch (23) is arranged at the connecting position between the box body (7) and the box cover (3).

3. The gut bacterial microorganism trap for insects according to claim 2, wherein, A support (24) is arranged in the box body (7), a plurality of limiting holes are arranged side by side on the support (24), and the disposable forceps heads (4) are clamped in each limiting hole.

4. The gut bacterial microbe trap for insects according to claim 1, wherein, A clamping member for stable connection is further arranged on the connecting head (9), the clamping member is a triangular clamping block (25), and a clamping groove corresponding in size to the triangular clamping block (25) is further arranged on the inner wall of the sharp body (6).

5. The gut bacterial microbe trap for insects according to claim 1, wherein, The first sliding slot (26) is arranged on the side wall of the tweezer rod (10) along the length direction of the tweezer rod (10), one end of the control plate (17) is fixedly connected with the control rod (14), and the other end penetrates through the first sliding slot (26) and extends outward.

6. The gut bacterial microbe trap for insects according to claim 1, wherein, The second sliding slot (27) is symmetrically arranged on the side wall of the tweezer rod (10) along the length direction of the tweezer rod (10), and the two ends of the cross rod (15) respectively penetrate through two second sliding slots (27) and slide in the second sliding slots (27).

7. The bacterial microorganism trap for the gut of an insect according to claim 1, wherein The anti-skid pattern is arranged on the handle (11).

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