DNA synthesizer and synthesis method

Abstract

The present invention relates to the technical field of DNA synthesis, and specifically to a DNA synthesizer and a synthesis method, comprising: a nozzle printing device; a shower and air drying device; a protective device; a bottom plate printing device; and a positive pressure gas source and a negative pressure gas source, wherein the positive pressure gas source is connected to the nozzle and the air knife assembly, and the negative pressure gas source is connected to the exhaust pipeline, the liquid discharge hole and the nozzle. The present invention realizes the motion control and electric drive control of the nozzle printing device mechanism through the coordinated use of a motion control card, an electric drive controller, a positive pressure gas source and a negative pressure gas source. The object to be cleaned on the workstation can be cleaned, and the functions of air showering and air drying can be realized; the bearing mechanism has the function of automatically adsorbing the object to be cleaned, and can realize the fixation of the object to be cleaned; it can prevent the cleaning liquid from contaminating other workstations and equipment; it can realize the rapid recovery of waste liquid, quickly extract the waste liquid, and prevent it from accumulating on the bearing mechanism; and it can extract the residual gas, reduce the impact on the external environment, and prevent the waste liquid steam from polluting the working environment.

Description

Technical Field

[0001] The present invention relates to the technical field of DNA synthesis, in particular to a DNA synthesizer and a synthesis method. Background Art

[0002] A DNA synthesizer is an instrument that can automatically perform chemical synthesis and automatically connect amino acids and / or nucleotides according to design.

[0003] Before performing relevant operations on the substrate, the substrate needs to be cleaned, because the cleanliness of the substrate itself has a crucial impact on the subsequent operations and the quality of the product after the operation. However, in the existing cleaning device, after cleaning the substrate, liquid will remain on the supporting mechanism that supports the substrate, and steam gas will be generated during the cleaning process. The residual liquid and steam gas will pollute the working environment, and the random discharge of the residual liquid and gas generated during the cleaning process will also pollute the external environment.

[0004] At the same time, during the cleaning process, the high-pressure cleaning liquid will impact the substrate, causing the substrate to shift or even fall off the supporting mechanism.

[0005] However, if a structure is provided to prevent the accumulation of residual liquid and the discharge of steam, the structure and control of the DNA synthesizer will become more complicated, making it difficult to produce, assemble and use the DNA synthesizer. Summary of the invention

[0006] The purpose of the present invention is to solve the deficiencies of the prior art and to provide a DNA synthesizer and a synthesis method to solve at least one of the following technical problems: the problem that residual liquid accumulates on the lower side of the product after cleaning and is difficult to recover immediately; the problem of pollution to the working environment after cleaning; the problem of waste liquid and waste gas emissions polluting the external environment; and the problem that the product is difficult to fix during the cleaning process.

[0007] In order to achieve the above-mentioned purpose, a DNA synthesizer is designed, comprising: a nozzle printing device, comprising a nozzle motion module, a nozzle signal driving module, a nozzle air path module, a liquid storage bottle group and a nozzle connected to the liquid storage bottle group; a shower and air drying device, comprising an air knife assembly and a cleaning nozzle assembly; a protective device, wherein the protective device comprises a protective cover arranged in the circumference of the shower and air drying device, and an exhaust pipeline connected to the protective cover; a bottom plate printing device arranged at the lower side of the nozzle printing device and the shower and air drying device, comprising a motion mechanism and a bearing mechanism connected to the motion mechanism, the bearing mechanism is provided with a bearing position for bearing a product, a drainage hole is provided in the bearing position, and the motion mechanism can drive the bearing mechanism to move between the nozzle printing device and the shower and air drying device; and a positive pressure air source and a negative pressure air source, wherein the positive pressure air source is connected to the nozzle and the air knife assembly, and the negative pressure air source is connected to the exhaust pipeline, the drainage hole and the nozzle.

[0008] The present invention also has the following preferred technical solutions:

[0009] A pressure reducing valve and a pressure sensor are provided between the positive pressure air source and the nozzle and air knife assembly.

[0010] Electromagnetic valves are respectively arranged between the negative pressure source and the air extraction pipeline, the liquid discharge hole and the nozzle.

[0011] The nozzle is connected with a three-way electromagnetic valve, and the other two passages of the three-way electromagnetic valve are respectively connected with a positive pressure gas source and a negative pressure gas source.

[0012] The nozzle printing device also includes a numerical observation mechanism for observing the printing results.

[0013] A circumferential air duct is arranged on the inner side of the protective cover, and the air outlet direction of the circumferential air duct is obliquely pointed from top to bottom in the direction of the protective mechanism.

[0014] The bottom of the bearing position is provided with an inclined liquid discharge surface, and the bottom of the liquid discharge surface is provided with the liquid discharge hole.

[0015] A plurality of support platforms for supporting the objects to be cleaned are arranged in the bearing position, and retaining edges are arranged on the support platforms corresponding to the edges of the objects to be cleaned.

[0016] A negative pressure adsorption component is provided in the bearing position, and the negative pressure adsorption component is connected to a negative pressure gas source for fixing the product.

[0017] The present invention also relates to a DNA synthesis method of the DNA synthesizer, which is described as follows: fixing the product by a base plate printing device and moving the product to a nozzle printing device and a shower and air-drying device; performing DNA synthesis by the nozzle printing device, and adjusting the printing pressure of the nozzle by a positive pressure air source and a negative pressure air source; showering and air-drying the product after DNA synthesis by the shower and air-drying device, and driving the wind knife assembly by the positive pressure air source; limiting the circulation range of the gas and liquid generated during the shower and air-drying process by the protective device, and driving the exhaust pipeline by the negative pressure air source to achieve exhaust and drive the liquid to discharge through the drain hole.

[0018] Advantageous Effects of the Invention

[0019] Compared with the prior art, the present invention has a simple and feasible combined structure, is easy to install and disassemble, and has the advantages of: through the coordinated use of a motion control card, an electric drive controller, a positive pressure gas source, and a negative pressure gas source, the motion control and electric drive control of the nozzle printing device mechanism are realized. The present invention can clean the objects to be cleaned on the workstation through the coordinated use of a positive pressure gas source and a negative pressure gas source, and can realize the functions of air showering and air drying; the supporting mechanism has the function of automatically adsorbing the objects to be cleaned, and can realize the absolute fixation of the objects to be cleaned; it can prevent the cleaning liquid from contaminating other workstations and equipment; it can realize the rapid recovery of waste liquid, and can quickly extract the waste liquid to prevent accumulation in the supporting mechanism; and it can extract the residual gas to reduce the impact on the external environment and prevent the waste liquid steam from polluting the working environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The front view of the DNA synthesizer of the present invention is exemplarily shown.

[0021] Figure 2 A schematic diagram of a nozzle printing module of the present invention is exemplarily shown.

[0022] Figure 3 The schematic diagram of the shower and air-drying equipment and the protective equipment of the present invention is exemplified.

[0023] Figure 4 A schematic diagram exemplarily shows a bottom plate printing device of the present invention.

[0024] Figure 5 A schematic diagram exemplarily shows a button control device of the present invention.

[0025] Figure 6 A partial connection diagram of the positive pressure gas source of the present invention is exemplarily shown.

[0026] Figure 7 A partial connection diagram of the negative pressure gas source of the present invention is exemplarily shown.

[0027] Figure 8 The schematic diagram of pressure regulation of the spray head is shown as an example.

[0028] Fig. 9 A schematic diagram showing a positive pressure gas source connected to a liquid circuit nozzle.

[0029] In the figure: 1. glove box 2. nozzle printing equipment 21. vertical observation camera 22. signal line terminal board 23. liquid storage bottle group 24. nozzle mounting block 25. nozzle 3. shower and air drying equipment 31. mounting plate 32. air duct 33. air knife 34. liquid path fixing pipe 4. bottom plate printing equipment 41. horizontal observation camera 42. XY motion module 43. bearing mechanism 44. strobe light module 5. button control equipment 51. main switch 52. pressure sensor 53. air pressure regulating valve 54. pressure reducing valve 6. reagent bottle group 7. electrical cabinet 8. protective equipment. DETAILED DESCRIPTION

[0030] The present invention is further described below in conjunction with the accompanying drawings, and the structure and principle of the device and method are very clear to people in the field. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0031] The present invention provides a DNA synthesizer, comprising: a nozzle printing device 2, comprising a liquid storage bottle group 23 and a nozzle 25 connected to the liquid storage bottle group 23; a shower and air drying device 3, comprising an air knife assembly and a cleaning nozzle assembly; a protective device 8, wherein the protective device 8 comprises a protective cover arranged in the circumference of the shower and air drying device 3, and an exhaust pipeline connected to the protective cover; a bottom plate printing device 4 arranged at the lower side of the nozzle printing device 2 and the shower and air drying device 3, comprising a moving mechanism and a bearing mechanism 43 connected to the moving mechanism, wherein the bearing mechanism 43 is provided with a bearing position for bearing a product, wherein a drainage hole is provided in the bearing position, and the moving mechanism can drive the bearing mechanism 43 to move between the nozzle printing device 2 and the shower and air drying device 3; and a positive pressure air source and a negative pressure air source, wherein the positive pressure air source is connected to the nozzle 25 and the air knife assembly, and the negative pressure air source is connected to the exhaust pipeline, the drainage hole and the nozzle 25.

[0032] When in use, the product is fixed by the base plate printing device 4 and moved to the nozzle printing device 2 and the shower and air-drying device 3; DNA synthesis is performed by the nozzle printing device 2, and the printing pressure of the nozzle 25 is adjusted by the positive pressure air source and the negative pressure air source; the product after DNA synthesis is showered and air-dried by the shower and air-drying device 3, and the air knife assembly is driven by the positive pressure air source; the circulation range of the gas and liquid generated during the shower and air-drying process is limited by the protective device 8, and the vacuum pipeline is driven by the negative pressure air source to achieve vacuum and drive the liquid to discharge through the drain hole.

[0033] See also Figure 1 As shown, in some embodiments, the DNA synthesizer includes a glove box 1, a nozzle printing device 2, a shower and air drying device 3, a protective device 8, a bottom plate printing device 4 arranged at the lower side of the nozzle printing device 2 and the shower and air drying device 3, a button control device 5, a reagent bottle group 6 and an electrical cabinet 7.

[0034] The glove box 1 is used to place gloves, the electrical cabinet 7 is used to place related control components, circuit structures and power supply components, etc., and the reagent bottle group 6 is reagent bottles to be used.

[0035] The nozzle printing module 2 includes a main body for installing printing accessories and outputting air circuits, and also includes a nozzle motion module, a nozzle air circuit module, a nozzle signal driving module, a liquid storage bottle group 23 and a nozzle connected to the liquid storage bottle group 23, wherein the nozzle motion module is used to drive the nozzle to move to achieve printing, the nozzle air circuit module is used to achieve the connection between the pressure source and the nozzle, the nozzle signal driving module is used to control the use of the nozzle, for example, including a motion control card and an electric drive controller, and the liquid storage bottle group 23 is used to provide corresponding liquid to the nozzle. For example, see Figure 2 As shown, the nozzle printing module 2 also includes a liquid storage bottle group 23, a nozzle mounting block 24, a nozzle 25 and a signal line terminal block 22. The nozzle mounting block 24 is used to install and connect the nozzle 25. The nozzle 25 is connected to the liquid storage bottle group 23. The signal line terminal block 22 is connected to the nozzle 25 signal and is connected to the electrical cabinet 7 signal to realize the control of the nozzle 25.

[0036] In a preferred embodiment, the nozzle printing module 2 further includes a vertical observation camera 21 for observing the printing result.

[0037] See also Figure 3As shown, the shower and air-drying equipment 3 includes a mounting plate 31, an air duct 32, an air knife 33 and a liquid path fixed pipe 34, wherein the mounting plate 31 is used to mount various components, the air duct 32 is connected to the air knife 33 and is used to connect to a positive pressure gas source, and a liquid path pipe and a liquid path nozzle 25 are fixed in the liquid path fixed pipe 34, wherein the air knife 33 is used to achieve air drying, and the liquid path pipe and the liquid path nozzle 25 are used to achieve showering, thereby achieving the combined shower and air-drying function.

[0038] The protective device 8 includes a protective cover arranged in the circumference of the shower and air-drying device 3, and an exhaust pipeline connected to the protective cover. The exhaust pipeline is connected to a negative pressure gas source and is used to extract the gas in the protective cover after the shower and air-drying is completed.

[0039] In a preferred embodiment, a circumferential air duct 32 is further provided on the inner side of the protective cover, and the air outlet direction of the circumferential air duct 32 is obliquely pointed from top to bottom in the direction of the protective mechanism, so that the residual liquid in the protective cover can be blown downward to the supporting mechanism 43.

[0040] See also Figure 4 As shown, the base plate printing device 4 includes an XY motion module 42 and a bearing mechanism 43. The bearing mechanism 43 is arranged on the XY motion module 42 and can move under the drive of the XY motion module 42. The bearing mechanism 43 is used to load the substrate.

[0041] In a preferred embodiment, a horizontal observation camera 41 and a strobe light module 44 are disposed on opposite sides of the supporting mechanism 43 to observe the state of the droplets before printing.

[0042] See also Figure 5 As shown, the button control device 5 includes a main switch 51, a pressure sensor 52 switch, a gas pressure regulating valve 53 switch, a pressure reducing valve 54 switch, etc., and is mainly used to install a power switch and sensors of various gas and liquid circuits and switches of regulating valves, etc. In a preferred embodiment, a relay is provided in the electrical cabinet, and the relay is connected to the button control device 5 to realize the control of various components.

[0043] See also Figure 6 As shown, the positive pressure air source is output to the air outlet of the wind knife 33, the shower and drying equipment and the protective equipment 8 after the air pressure is adjusted by the pressure reducing valve 54, and a solenoid valve is also provided for opening and closing and / or flow control, and the solenoid valve control switch is arranged at the button control device 5.

[0044] See also Figure 7 As shown, the negative pressure gas source is connected to the air extraction pipeline and the liquid discharge hole through the solenoid valve, so that the waste liquid can be sucked away, and the solenoid valve control switch is arranged at the button control device 5.

[0045] See also Figure 8 As shown, the positive pressure air source and the negative pressure air source are also connected to the nozzle 25. The nozzle 25 requires three kinds of air pressure to work - positive pressure and negative pressure for cleaning, and regulating pressure for printing. Among them, the regulating pressure is generated after the positive pressure and negative pressure are input together according to demand. An air triplet is provided on the rear side of the positive pressure air source to filter and adjust the air pressure, and then it is divided into two channels connected to the regulating hand valve and the positive pressure channel respectively, and the regulating hand valve can adjust the air pressure. The negative pressure air source is divided into two channels after being connected to the regulating hand valve and the negative pressure channel respectively, and the regulating hand valve can adjust the air pressure. In addition, since there will be residual air pressure inside the pipeline when the air pressure of the nozzle 25 is switched, a two-position three-way solenoid valve is added on the front side of the nozzle 25 to relieve pressure. The three passages of the three-way solenoid valve are respectively connected to the nozzle 25, the positive pressure channel and the negative pressure channel.

[0046] See also Fig. 9 As shown, Fig. 9 Taking one liquid circuit as an example, the positive pressure air source is connected to the air triplex to filter and adjust the air pressure, and then connected to the reagent bottle through the pressure reducing valve 54 to flush the solution inside the reagent bottle to the solenoid valve switch. After turning on the switch, the solution can reach the liquid circuit nozzle 25 of the shower and air-drying equipment 3 for showering. Example

[0047] This embodiment shows a structure and use example of a shower and air-drying device 3, a protective device 8 and a base plate printing device 4.

[0048] When in use, the showering and air-drying operations are performed through the showering and air-drying equipment 3, the circulation range of the cleaning liquid and the gas generated during the cleaning process is limited by the protective cover, and the cleaning liquid on the inner wall of the protective cover is blown to the supporting mechanism 43 through the circumferential air duct 32 arranged on the inner side of the protective cover. The product to be carried is carried by the supporting mechanism 43, and the cleaning liquid is collected by the drainage surface and then discharged through the drainage hole.

[0049] The cleaning mechanism comprises a base, on which an air knife assembly and a cleaning nozzle assembly are arranged.

[0050] The wind knife assembly includes a wind knife 33 and a wind duct 32 connected to the wind knife 33. The wind knife 33 is preferably provided with a flat air outlet.

[0051] The cleaning nozzle assembly includes: a plurality of liquid pipes and liquid nozzles; a liquid pipe mounting hole located on the base, the liquid pipe mounting hole corresponding to the liquid pipe; a liquid pipe clamp structure located on the lower side of the base, the liquid pipe clamp structure is provided with a liquid pipe positioning hole corresponding to the liquid pipe mounting hole.

[0052] For example, the base is provided with 6-way liquid tube mounting holes and clamp mounting holes, the clamp mounting holes are used to connect the liquid tube clamp structure, the inner cross-section of the liquid tube clamp structure, that is, the internal cross-section, gradually shrinks from top to bottom so as to accommodate and constrain the liquid tube, and the bottom of the liquid tube clamp structure is also provided with 6-way liquid tube positioning holes, the pattern formed by the 6-way liquid tube positioning holes forms a similar pattern to the pattern formed by the 6-way liquid tube mounting holes, and the size of the pattern formed by the 6-way liquid tube positioning holes is smaller than the pattern formed by the 6-way liquid tube mounting holes, and the 6-way liquid tube and the liquid nozzle pass through the 6-way liquid tube mounting holes and the 6-way liquid tube positioning holes in sequence and are fixed.

[0053] Thus, through the 6-way liquid pipe and nozzle, the corresponding solution is sprayed from the liquid supply system to the upper side of the carrier mechanism 43, so as to achieve comprehensive cleaning of each position on the surface of the object to be cleaned. Then, the wind knife 33 will blow out a strong wind under the action of the positive pressure pump, and cooperate with the horizontal movement of the carrier mechanism 43 to blow all the residual liquid on the bottom plate away from the upper surface of the object to be cleaned to achieve a comprehensive air drying function.

[0054] In a preferred embodiment, the protective cover is further provided with an exhaust pipeline for extracting the waste gas after cleaning and drying in the protective cover. For example, the base is further provided with an air suction hole, which is connected to the space in the protective cover, and the air suction hole is connected to the waste gas treatment system through an air pipeline, and the air pipeline is connected to a negative pressure device such as an air pump, so that the waste gas generated in the local space after cleaning and drying is quickly absorbed into the waste gas treatment system by negative pressure, which will play a role in reducing the pollution of the working environment by waste liquid steam.

[0055] The glass cover of the protective device 8 is arranged around the cleaning device, for example, the glass cover is connected to the base of the cleaning device via connecting bolts. Therefore, during the cleaning and air-drying process, the glass cover can ensure that the liquid will not be blown into the entire working environment or droplets will be blown onto other workstations and the bottom plate between the workstations, thereby preventing the cleaning liquid from contaminating other workstations.

[0056] Preferably, a guide rail is provided on the bearing mechanism 43 along the direction of the linear motion of the glass cover, the length of the glass cover in the direction of the linear motion is longer than the length of the guide rail, and the glass cover can move along the guide rail driven by the mobile driving mechanism.

[0057] At the same time, the setting of the glass cover also plays a role in limiting the flow range of waste liquid. The waste liquid generated during the cleaning process cannot flow out of the limited range of the glass cover, so it can be effectively collected by the bearing position of the lower bearing mechanism 433 and discharged from the drain port.

[0058] However, during the cleaning and air-drying process, waste liquid will splash onto the inner wall of the glass cover. When not cleaning and air-drying, the cleaning mechanism and the glass cover will be lifted up to the waiting position. When in the waiting position, if there are too many liquid droplets inside the glass cover, there is a risk of dripping, and there may be corrosion-resistant equipment such as motors directly below it, which will cause serious damage to other parts, so it is very important to keep the inside clean.

[0059] Therefore, the circumferential air duct 32 is provided on the inner side of the glass cover to blow the liquid droplets from the glass cover to the bearing position of the support. In a preferred embodiment, an annular multi-channel air duct 32 is provided on the base of the cleaning mechanism at a position corresponding to the inner side of the glass cover as the circumferential air duct 32, and the annular multi-channel air duct 32 is connected to a positive pressure pump and other equipment to generate air. This air path blows from top to bottom, so that the liquid droplets hanging on the wall inside the glass cover are blown onto the bearing mechanism 43, so as to ensure the cleanliness of the inner wall of the glass cover and prevent contamination or damage to the substrate or other objects to be cleaned.

[0060] The carrying mechanism 43 is provided with a plurality of carrying positions for carrying objects to be cleaned, the bottom of each carrying position is provided with an inclined liquid discharge surface, and the bottom of each liquid discharge surface is provided with a liquid discharge hole for discharging waste liquid.

[0061] For example, the bottom of the bearing position of the bearing mechanism 43 is funnel-shaped, and the middle bottom end of the bearing position is the lowest end of the entire funnel-shaped bearing position. The circumference of the lowest end has a drainage surface that is inclined from top to bottom toward the lowest end, and the drainage hole is located at the lowest end of the funnel-shaped bearing position, that is, the middle of the bottom end.

[0062] When the cleaning liquid flows from the substrate to the bottom plate of the bearing position or flows from the glass cover to the bottom plate, the bottom plate of the bearing position has a drainage surface inclined toward the drainage hole, so that the cleaning liquid can be collected to the drainage hole. When the bottom plate of the bearing position is funnel-shaped, the cleaning liquid is collected at the drainage hole in the middle of the bottom. Preferably, through the negative pressure pump, the waste liquid collected at the bottom plate funnel can be quickly collected into the waste liquid bottle through the negative pressure pipeline. This process not only takes away the waste liquid collected on the funnel-shaped bottom plate, but also can suck away the residual liquid on the bottom plate through negative pressure, and finally achieves a state where there is no residual liquid on the bottom plate.

[0063] In some embodiments, a plurality of support platforms for supporting the objects to be cleaned are provided in the support position of the support mechanism 43, and a retaining edge is provided on the support platform corresponding to the edge of the objects to be cleaned. In a preferred embodiment, the support platform is arranged at the edge of the objects to be cleaned, for example, support platforms are provided at the four corners of the objects to be cleaned, and grooves sunken downward are provided on the support platform along the four corners of the objects to be cleaned, and the inner wall of the groove 34 serves as a retaining edge to limit the four corners of the objects to be cleaned, thereby positioning the objects to be cleaned.

[0064] Thus, by setting up four supports for placing the objects to be cleaned, it is ensured that the objects to be cleaned are not in direct contact with the bottom of the carrying mechanism 43, and the contact surface is reduced, which facilitates loading and unloading of the objects to be cleaned, and avoids that the solution on the surface of the objects to be cleaned cannot be completely cleaned during the cleaning and air-drying process. The setting of the retaining edge prevents the objects to be cleaned from slipping during the movement of the carrying mechanism 43 or during the cleaning and air-drying process, thereby achieving physical fixation and positioning of the objects to be cleaned.

[0065] In a preferred embodiment, a negative pressure adsorption component is further provided in the bearing position for fixing the object to be cleaned. For example, a negative pressure adsorption hole is provided in the downwardly concave groove, and the negative pressure adsorption hole is connected to a negative pressure gas source through an air passage, so that the object to be cleaned can be further fixed by the arrangement of the negative pressure adsorption component.

Claims

1. A DNA synthesizer, Features include: A nozzle printing device, comprising a liquid storage bottle group and a nozzle connected to the liquid storage bottle group; A shower and air-drying device, comprising an air knife assembly and a cleaning nozzle assembly, wherein the shower and air-drying device performs shower and air-drying on the product after DNA synthesis is completed; A protective device, the protective device comprising a protective cover arranged around the shower and air-drying device, and an exhaust pipeline connected to the protective cover; The bottom plate printing device disposed under the nozzle printing device and the shower and air-drying device comprises a motion mechanism and a bearing mechanism connected to the motion mechanism, wherein the bearing mechanism is provided with a plurality of bearing positions for bearing the product, and the bearing positions are provided with drainage holes, and the motion mechanism can drive the bearing mechanism to move between the nozzle printing device and the shower and air-drying device; and A positive pressure air source and a negative pressure air source, wherein the positive pressure air source is connected to the nozzle and the air knife assembly, and the negative pressure air source is connected to the air extraction pipeline, the drainage hole and the nozzle. A circumferential air duct is arranged on the inner side of the protective cover, and the air outlet direction of the circumferential air duct is obliquely pointed from top to bottom in the direction of the protective mechanism, so as to blow the droplets from the protective cover into the bearing position.

2. The DNA synthesizer according to claim 1, Features A pressure reducing valve and a pressure sensor are provided between the positive pressure air source and the nozzle and air knife assembly.

3. The DNA synthesizer according to claim 1, Features Solenoid valves are respectively arranged between the negative pressure gas source and the air extraction pipeline, the liquid discharge hole and the nozzle.

4. The DNA synthesizer according to claim 1, Features The nozzle is connected with a three-way electromagnetic valve, and the other two passages of the three-way electromagnetic valve are respectively connected with a positive pressure gas source and a negative pressure gas source.

5. The DNA synthesizer according to claim 1, Features The nozzle printing device also includes a numerical observation mechanism for observing the printing results.

6. The DNA synthesizer according to claim 1, Features The bottom of the bearing position is provided with an inclined liquid discharge surface, and the bottom of the liquid discharge surface is provided with the liquid discharge hole.

7. The DNA synthesizer according to claim 1, Features A plurality of support platforms for supporting the objects to be cleaned are arranged in the bearing position, and retaining edges are arranged on the support platforms corresponding to the edges of the objects to be cleaned.

8. The DNA synthesizer according to claim 1, Features A negative pressure adsorption component is provided in the bearing position, and the negative pressure adsorption component is connected to a negative pressure gas source for fixing the product.

9. A method for DNA synthesis using the DNA synthesizer according to claim 1, Features The method described is as follows: Fix the product through the base plate printing device and move the product to the nozzle printing device and the shower and air drying device; DNA synthesis is performed by a nozzle printing device, and the printing pressure of the nozzle is adjusted by a positive pressure gas source and a negative pressure gas source; The product after DNA synthesis is rinsed and air-dried by a rinse and air-drying device, and the air knife assembly is driven by the positive pressure air source; The protective device is used to limit the flow range of gas and liquid generated during the shower and air-drying process, and the negative pressure gas source is used to drive the air extraction pipeline to achieve air extraction and drive the liquid to be discharged through the drainage hole.

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