A kind of method and application of detecting single nucleotide polymorphism of sheep lipe gene

A single nucleotide polymorphism and sheep technology, applied in the field of molecular genetics detection, can solve problems such as early screening problems, and achieve the effect of speeding up the breeding process

CN113136439BActive Publication Date: 2022-04-08甘肃润牧生物工程有限责任公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2022-04-08

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Abstract

The invention discloses a method for detecting single nucleotide polymorphism of sheep LIPE gene and its application, comprising the following steps: extracting Hu sheep tissue DNA sample, single nucleotide polymorphism of LIPE gene mutation site g.4819A>G Morphological typing; Through the association analysis of the single nucleotide polymorphism of the LIPE gene of Hu sheep and the content of intramuscular fat, it shows that the typing detection of the single nucleotide polymorphism of the LIPE gene of Hu sheep can be used as an improvement. Molecular markers of intramuscular fat content in Hu sheep can be used for breeding sheep breeds with good meat quality.
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Description

technical field

[0001] The invention belongs to the field of molecular genetics detection, and relates to a method for detecting the single nucleotide polymorphism of sheep LIPE gene by using DNA pool sequencing combined with imLDR technology and an application thereof. Background technique

[0002] Single nucleotide polymorphism (Single nucleotide polymorphism, SNP) refers to the variation caused by the conversion, transversion, insertion and deletion of a single base at the genome level. SNPs widely exist in the genome and are widely used in the construction of genetic maps, precise gene positioning, and genetic breeding. SNP can be divided into SNP (coding SNP, cSNP) in the coding region of the gene and SNP in the non-coding region according to the location of the mutation. cSNP is divided into synonymous cSNP and non-synonymous cSNP. A synonymous cSNP refers to a base mutation that does not cause a change in the amino acid sequence of the protein. Non-synonymous cSNP r...

Examples

Embodiment Construction

[0027] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. The examples are only used to explain the technical solutions of the present invention, rather than to limit the protection scope of the present invention.

[0028] (1) Screening of new single nucleotide mutation sites in the LIPE gene of Hu sheep by DNA pool sequencing

[0029] S1 sample selection:

[0030] From August 2018 to January 2020, a total of 921 (four batches) of healthy Hu sheep at the age of 6 months were collected from Minqin Defu Agricultural Technology Co., Ltd. under the same feeding conditions. Determination of intramuscular fat content of the longissimus dorsi muscle of individuals.

[0031] Construction of S2 DNA pool:

[0032] a) Using the high-salt method to extract DNA (genomic DNA) from 921 individual Hu sheep testis tissues, the specific steps are:

[0033] Ⅰ) After grinding the tissue sample under liquid nitrogen, t...