A horizontal rotating drum animal cell culture reactor with a magnetic stirring system

Abstract

The present invention discloses a horizontal rotary drum type animal cell culture reactor with a magnetic stirring system, comprising a reactor shell, a support cylinder fixedly connected to the inner side of the reactor shell, a support buffer mechanism provided on the inner side of the support cylinder, and the support cylinder is connected to a horizontal stirring cylinder through the support buffer mechanism, and a magnetic stirring system is provided at one end of the horizontal stirring cylinder; through the designed main electromagnetic driving device, stirring seat, stirring blade, push-type stirring blade and small electromagnetic driving device, when in use, the stirring seat and the stirring blade are driven to rotate by the main electromagnetic driving device, so as to stir the culture material and cells, and the bending arc at one end of the stirring main blade of the stirring blade is cooperated to reduce the shear force generated during stirring, and the push-type stirring blade is driven to rotate by the small electromagnetic driving device to push the culture material and cells to move, so as to cooperate with the stirring blade to stir, so that the stirring is faster and more uniform.

Description

Technical Field

[0001] The invention belongs to the technical field of animal cell culture, and in particular relates to a horizontal rotary drum type animal cell culture reactor with a magnetic stirring system. Background Art

[0002] The nutrients required for animal cell culture are basically the same as those in the body, for example, sugars, amino acids, inorganic salts, growth factors, trace elements, etc. The culture medium that strictly formulates the above substances required by cells according to their types and required quantities is called synthetic culture medium. Since some components of the internal environment of animal cells have not been studied clearly, animal serum needs to be added to provide an environment similar to that in the body. Therefore, when using synthetic culture medium, some natural ingredients such as serum and plasma are usually added.

[0003] When using existing animal cell culture reactors, stirring is usually used to mix the culture materials. During the mixing, due to the excessive shear force of the stirring shaft, cells and the like may be damaged by the stirring, and the mixing effect is poor during the stirring and mixing, resulting in the problem of poor mixing effect. For this reason, we propose a horizontal rotating drum animal cell culture reactor with a magnetic stirring system. Summary of the invention

[0004] The object of the present invention is to provide a horizontal rotary drum animal cell culture reactor with a magnetic stirring system to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a horizontal rotary drum type animal cell culture reactor with a magnetic stirring system, comprising a reactor shell, a support cylinder fixedly connected to the inner side of the reactor shell, a support buffer mechanism provided on the inner side of the support cylinder, and the support cylinder is connected to a horizontal stirring drum through the support buffer mechanism, a magnetic stirring system is provided at one end of the horizontal stirring drum, the magnetic stirring system comprises a main electromagnetic driving device installed on the outer side of one end of the horizontal stirring drum and an installation box provided on the inner side of the other end of the horizontal stirring drum, a small electromagnetic driving device is fixedly connected to the inner side of the installation box, a push-type stirring blade is rotatably connected to one side of the installation box, and the push-type stirring blade is fixedly connected to the output shaft of the small electromagnetic driving device, the output shaft of the main electromagnetic driving device is fixedly connected to a stirring seat, a stirring blade is provided on the outer surface of the stirring seat, the stirring blade comprises a stirring main blade, one end of the stirring main blade is arranged in an arc shape, a reinforcing rib is provided at the connection between the stirring main blade and the stirring seat, and a stirring hole is provided on the surface of the stirring main blade.

[0006] Preferably, the support buffer mechanism includes a support rib plate fixedly installed between the horizontal mixing drum and the support drum, a pushing block is provided at the inner middle part of the support rib plate, a fixed block is fixedly connected to the inner surface of the support drum, a buffer spring is fixedly connected to one side of the fixed block, a sliding block is fixedly connected to one end of the buffer spring, and the sliding block is slidably connected to the support drum, and the position of the sliding block corresponds to the position of the pushing block.

[0007] Preferably, one side of the sliding block and the lower end of the pushing block are both provided with inclined surfaces, and the pushing block and the sliding block are fitted together via the inclined surfaces.

[0008] Preferably, a groove is provided on the surface of the support tube at a position corresponding to the pushing block, and the shape of the groove matches the shape of the lower end of the pushing block.

[0009] Preferably, the shape of the supporting rib is set to be U-shaped, and the fixing block, the buffer spring and the sliding block are located on the inner side of the supporting rib.

[0010] Preferably, the stirring blade is centrally symmetrically installed on the outer surface of the stirring seat, the push-type stirring blade is located at the inner bottom of the horizontal stirring drum, and the direction of the push-type stirring blade corresponds to the position of the stirring blade.

[0011] Preferably, a supporting foot is provided at the lower end of the reactor shell, and an adjusting mechanism is provided inside the lower end of the reactor shell at a position corresponding to the supporting foot.

[0012] Preferably, the adjustment mechanism includes a sliding box slidably installed inside the lower end of the reactor shell and an adjusting screw rotatably installed on the lower side of the sliding box, the supporting foot is installed at the lower end of the adjusting screw, and the lower end of the reactor shell is fixedly connected with an adjusting nut, and the adjusting nut is screwed on the adjusting screw through a thread.

[0013] Preferably, a transmission worm is rotatably connected to the interior of the sliding box via a bearing, one end of the transmission worm is fixedly connected to an adjusting handwheel, one side of the transmission worm is meshed with a transmission worm wheel, and the transmission worm wheel is fixedly mounted on the upper end of the adjusting screw.

[0014] Preferably, a rubber ring is provided on one side of the reactor shell at a position corresponding to the horizontal stirring drum, and the rubber ring is sleeved on one end of the horizontal stirring drum.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. Through the designed main electromagnetic drive device, stirring seat, stirring blade, push-type stirring blade and small electromagnetic drive device, when in use, the stirring seat and stirring blade are driven to rotate by the main electromagnetic drive device, so as to stir the culture material and cells, and the curved arc at one end of the stirring main blade of the stirring blade is used to reduce the shear force generated during stirring, and the small electromagnetic drive device is used to drive the push-type stirring blade to rotate, so as to push the culture material and cells to move, so as to cooperate with the stirring blade for stirring, so that the stirring is faster and more uniform.

[0017] 2. Through the designed support tube and support buffer mechanism, the horizontal mixing drum is fixed and supported by the support tube and the support buffer mechanism during use. The vibration generated during use can be supported and buffered by the support rib plate of the support buffer mechanism, and the sliding block is pushed by the push block to slide and compress the buffer spring to assist in buffering, thereby making the buffering effect better.

[0018] 3. Through the designed adjustment mechanism, the position of the supporting feet can be adjusted during use, so that when the reactor is placed and used, it can be adjusted according to the flatness of the ground, so that the reactor shell is placed more stably and avoids shaking and instability. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural schematic diagram of the present invention;

[0020] Figure 2 It is a schematic diagram of the installation structure of the horizontal mixing drum of the present invention;

[0021] Figure 3 It is a schematic diagram of the stirring blade structure of the present invention;

[0022] Figure 4 It is a schematic diagram of the side view structure of the support tube of the present invention;

[0023] Figure 5 For the present invention Figure 2 The enlarged structural diagram at A in the middle;

[0024] Figure 6 It is a schematic diagram of the structure of the regulating mechanism of the present invention;

[0025] In the figure: 1. Reactor shell; 2. Horizontal stirring drum; 3. Adjusting hand wheel; 4. Adjusting mechanism; 5. Support foot; 6. Main electromagnetic drive device; 7. Stirring seat; 8. Stirring blade; 9. Mounting box; 10. Small electromagnetic drive device; 11. Push-type stirring blade; 12. Support drum; 13. Support buffer mechanism; 14. Main stirring blade; 15. Stirring hole; 16. Reinforcing rib; 17. Support rib plate; 18. Push block; 19. Sliding block; 20. Buffer spring; 21. Fixed block; 22. Sliding box; 23. Transmission worm gear; 24. Transmission worm; 25. Adjusting screw; 26. Adjusting nut. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] See also Figures 1 to 6 The present invention provides a technical solution: a horizontal rotary drum type animal cell culture reactor with a magnetic stirring system, comprising a reactor shell 1, a support cylinder 12 is fixedly connected to the inner side of the reactor shell 1, a support buffer mechanism 13 is arranged on the inner side of the support cylinder 12, and the support cylinder 12 is connected to a horizontal stirring cylinder 2 through the support buffer mechanism 13, one end of the horizontal stirring cylinder 2 is provided with a magnetic stirring system, the magnetic stirring system comprises a main electromagnetic drive device 6 installed on the outer side of one end of the horizontal stirring cylinder 2 and an installation box 9 arranged on the inner side of the other end of the horizontal stirring cylinder 2, a small electromagnetic drive device 10 is fixedly connected to the inner side of the installation box 9, a push-type stirring blade 11 is rotatably connected to one side of the installation box 9, and the push-type stirring blade 11 is fixedly connected to the output shaft of the small electromagnetic drive device 10, the small electromagnetic drive device 10 drives the push-type stirring blade 11 to rotate, and the main electromagnetic drive device 6 The output shaft is fixedly connected to a stirring seat 7, and a stirring blade 8 is arranged on the outer surface of the stirring seat 7. The stirring blade 8 includes a stirring main blade 14. When in use, the push-type stirring blade 11 pushes the material and cells to move toward the stirring blade 8, thereby facilitating the stirring of the stirring blade 8, and making the stirring more uniform and efficient. One end of the stirring main blade 14 is arranged in an arc shape, and a reinforcing rib 16 is arranged at the connection between the stirring main blade 14 and the stirring seat 7. A stirring hole 15 is opened on the surface of the stirring main blade 14. The arc shape of one end of the stirring main blade 14 on the stirring blade 8 reduces the shear force of stirring, and cooperates with the stirring hole 15 for rapid stirring.

[0028] From the above description, it can be seen that the present invention has the following beneficial effects: the main electromagnetic drive device 6 drives the stirring seat 7 and the stirring blade 8 to rotate, so as to stir the culture material and cells, and the curved arc at one end of the stirring main blade 14 of the stirring blade 8 reduces the shear force generated during stirring, and the small electromagnetic drive device 10 drives the push-type stirring blade 11 to rotate, pushing the culture material and cells to move, thereby cooperating with the stirring blade 8 for stirring, making the stirring faster and more uniform.

[0029] For further information, see Figure 2 , Figure 4 and Figure 5The support and buffer mechanism 13 includes a support rib 17 fixedly installed between the horizontal mixing drum 2 and the support drum 12, a pushing block 18 is arranged at the inner middle part of the support rib 17, a fixed block 21 is fixedly connected to the inner surface of the support drum 12, a buffer spring 20 is fixedly connected to one side of the fixed block 21, a sliding block 19 is fixedly connected to one end of the buffer spring 20, and the sliding block 19 is slidably connected to the support drum 12, and the position of the sliding block 19 corresponds to the position of the pushing block 18. The support and buffering are performed by the support rib 17 of the support and buffer mechanism 13, and at the same time, when the vibration force is too large, the pushing block 18 is driven to push the sliding block 19 to slide and compress the buffer spring 20 to assist in buffering, so as to better buffer and protect the horizontal mixing drum 2. An inclined surface is arranged on one side of the sliding block 19 and the lower end of the pushing block 18, and the pushing block 18 and the sliding block 19 are fitted together through the inclined surface, and the surface of the support drum 12 corresponds to the pushing block 18. A groove is provided at the position, and the shape of the groove matches the shape of the lower end of the push block 18. The shape of the support rib plate 17 is set to be U-shaped. The fixed block 21, the buffer spring 20 and the sliding block 19 are located on the inner side of the support rib plate 17, so that the support rib plate 17 has a better supporting effect when in use.

[0030] For further information, see Figure 2 The stirring blade 8 is centrally symmetrically installed on the outer surface of the stirring seat 7, the push-type stirring blade 11 is located at the inner bottom of the horizontal stirring drum 2, and the direction of the push-type stirring blade 11 corresponds to the position of the stirring blade 8, which facilitates the stirring of the stirring blade 8 and the push-type stirring blade 11 and speeds up the stirring efficiency.

[0031] For further information, see Figure 1 and Figure 6 A supporting foot 5 is provided at the lower end of the reactor shell 1, and an adjusting mechanism 4 is provided at a position corresponding to the supporting foot 5 inside the lower end of the reactor shell 1. The adjusting mechanism 4 includes a sliding box 22 slidably installed inside the lower end of the reactor shell 1 and an adjusting screw 25 rotatably installed on the lower side of the sliding box 22. The supporting foot 5 is installed at the lower end of the adjusting screw 25. An adjusting nut 26 is fixedly connected to the lower end of the reactor shell 1, and the adjusting nut 26 is screwed on the adjusting screw 25 through a thread. A transmission worm 24 is rotatably connected to the inside of the sliding box 22 through a bearing, and one end of the transmission worm 24 is fixedly connected to an adjusting handwheel 3, and a transmission worm wheel 23 is meshed with one side of the transmission worm 24, and the transmission worm wheel 23 is fixedly installed on the upper end of the adjusting screw 25.

[0032] For further information, see Figure 1 and Figure 2 A rubber ring is provided on one side of the reactor shell 1 at a position corresponding to the horizontal stirring drum 2 , and the rubber ring is sleeved on one end of the horizontal stirring drum 2 .

[0033] The electromagnetic drive device of the present invention is a permanent magnet synchronous motor, which has the characteristics of high control accuracy, high torque density, good torque stability and low noise. By reasonably designing the permanent magnet magnetic circuit structure, higher weak magnetic properties can be obtained, thereby improving the speed regulation range of the motor.

[0034] The working principle and use process of the present invention are as follows: when in use, according to the flatness of the ground, the transmission worm 24 is driven to rotate by turning the adjustment hand wheel 3, and the transmission worm wheel 23 is cooperated to drive the adjustment screw 25 to rotate, and the adjustment screw 25 and the adjustment nut 26 cooperate to make the adjustment screw 25 slide up and down to adjust the position of the support foot 5, so that the reactor shell 1 is placed more stably, and the culture material and cells are added into the horizontal stirring drum 2, and the stirring seat 7 and the stirring blade 8 are driven to rotate by the main electromagnetic drive device 6 to stir, and the arc shape of one end of the stirring main blade 14 on the stirring blade 8 is cooperated to reduce the stirring. The stirring shear force is generated, and the stirring hole 15 is used for rapid stirring. At the same time, the small electromagnetic drive device 10 drives the push-type stirring blade 11 to rotate, so that the push-type stirring blade 11 pushes the material and cells to move toward the stirring blade 8, thereby facilitating the stirring of the stirring blade 8, and the stirring is more uniform and efficient. When in use, the vibration generated by the horizontal stirring drum 2 is supported and buffered by the supporting rib plate 17 of the supporting buffer mechanism 13. At the same time, when the vibration force is too large, the pushing block 18 is driven to push the sliding block 19 to slide and compress the buffer spring 20 to assist in buffering, thereby better buffering and protecting the horizontal stirring drum 2.

[0035] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0036] The above description is only used to illustrate the technical solution of the present invention rather than to limit it. Other modifications or equivalent substitutions made to the technical solution of the present invention by ordinary technicians in this field should be included in the scope of the claims of the present invention as long as they do not depart from the spirit and scope of the technical solution of the present invention.

Claims

1. A horizontal rotating drum animal cell culture reactor with a magnetic stirring system, comprising a reactor housing (1), Features: A support cylinder (12) is fixedly connected to the inner side of the reactor shell (1), a support buffer mechanism (13) is provided on the inner side of the support cylinder (12), and the support cylinder (12) is connected to a horizontal stirring cylinder (2) via the support buffer mechanism (13), a magnetic stirring system is provided at one end of the horizontal stirring cylinder (2), a support foot (5) is provided at the lower end of the reactor shell (1), and an adjustment mechanism (4) is provided inside the lower end of the reactor shell (1) at a position corresponding to the support foot (5); The magnetic stirring system comprises a main electromagnetic drive device (6) installed on the outside of one end of a horizontal stirring drum (2) and a mounting box (9) arranged on the inside of the other end of the horizontal stirring drum (2); a small electromagnetic drive device (10) is fixedly connected to the inside of the mounting box (9); a push-type stirring blade (11) is rotatably connected to one side of the mounting box (9); and the push-type stirring blade (11) is fixedly connected to an output shaft of the small electromagnetic drive device (10); the output shaft of the main electromagnetic drive device (6) is fixedly connected to a stirring seat (7); a stirring blade (8) is arranged on the outer surface of the stirring seat (7); the stirring blade (8) comprises a stirring main blade (14); one end of the stirring main blade (14) is arranged in an arc shape; a reinforcing rib (16) is arranged at the connection between the stirring main blade (14) and the stirring seat (7); and a stirring hole (15) is opened on the surface of the stirring main blade (14); The stirring blade (8) is centrally symmetrically mounted on the outer surface of the stirring seat (7), the push-type stirring blade (11) is located at the inner bottom of the horizontal stirring drum (2), and the direction of the push-type stirring blade (11) corresponds to the position of the stirring blade (8).

2. A horizontal rotary drum animal cell culture reactor with a magnetic stirring system according to claim 1, Features: The support buffer mechanism (13) comprises a support rib plate (17) fixedly mounted between the horizontal mixing drum (2) and the support drum (12); a push block (18) is arranged at the inner middle portion of the support rib plate (17); a fixed block (21) is fixedly connected to the inner surface of the support drum (12); a buffer spring (20) is fixedly connected to one side of the fixed block (21); a sliding block (19) is fixedly connected to one end of the buffer spring (20); the sliding block (19) is slidably connected to the support drum (12); and the position of the sliding block (19) corresponds to the position of the push block (18).

3. The horizontal rotary drum animal cell culture reactor with a magnetic stirring system according to claim 2, Features: One side of the sliding block (19) and the lower end of the pushing block (18) are both provided with inclined surfaces, and the pushing block (18) and the sliding block (19) are fitted together via the inclined surfaces.

4. The horizontal rotary drum animal cell culture reactor with a magnetic stirring system according to claim 2, Features: A groove is provided on the surface of the support tube (12) at a position corresponding to the push block (18), and the shape of the groove matches the shape of the lower end of the push block (18).

5. The horizontal rotary drum animal cell culture reactor with a magnetic stirring system according to claim 2, Features: The shape of the supporting rib plate (17) is set to be U-shaped, and the fixed block (21), the buffer spring (20) and the sliding block (19) are located on the inner side of the supporting rib plate (17).

6. The horizontal rotary drum animal cell culture reactor with a magnetic stirring system according to claim 1, Features: The adjustment mechanism (4) comprises a sliding box (22) slidably mounted inside the lower end of the reactor housing (1) and an adjustment screw (25) rotatably mounted on the lower side of the sliding box (22); the support foot (5) is mounted on the lower end of the adjustment screw (25); the lower end of the reactor housing (1) is fixedly connected with an adjustment nut (26), and the adjustment nut (26) is screwed onto the adjustment screw (25) through a thread.

7. The horizontal rotary drum animal cell culture reactor with a magnetic stirring system according to claim 6, Features: A transmission worm (24) is rotatably connected to the interior of the sliding box (22) via a bearing, one end of the transmission worm (24) is fixedly connected to an adjustment hand wheel (3), one side of the transmission worm (24) is meshed with a transmission worm wheel (23), and the transmission worm wheel (23) is fixedly mounted on the upper end of the adjustment screw (25).

8. The horizontal rotary drum animal cell culture reactor with a magnetic stirring system according to claim 1, Features: A rubber ring is provided on one side of the reactor shell (1) at a position corresponding to the horizontal stirring drum (2), and the rubber ring is sleeved on one end of the horizontal stirring drum (2).

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