Filter membrane reaction cup and its usage method

Abstract

The present invention relates to a reaction cup with a filter membrane, which comprises a cup body. An inlet is provided at the upper end of the cup body, and an outlet is provided at the bottom of the cup body. A filter membrane is arranged at the outlet. When the outlet is under normal pressure, the bottom membrane is suitable for supporting the sample reaction solution in the cup body. When the outlet is under negative pressure, the sample reaction solution in the cup body is discharged through the outlet at the bottom of the cup, and the sample reaction solution is filtered through the filter membrane so that the microspheres in the sample reaction solution are retained in the cup body. A method for using a reaction cup with a filter membrane, wherein the bottom of the reaction cup is hermetically connected to a negative pressure liquid extraction device, and the negative pressure liquid extraction device is hermetically communicated with the cup cavity, and the sample reaction solution in the reaction cup is extracted by the negative pressure liquid extraction device. When removing the liquid in the cup body, the reaction cup with a filter membrane does not need to rely on a steel needle and a tip head, completely avoiding the carry-over pollution caused by using a steel needle to remove the liquid, and also avoiding the need to consume a large number of tip heads to remove the liquid during the experiment.

Description

Technical Field

[0001] The present invention relates to the field of tubular luminescence systems, and particularly to a reaction cup with a filter membrane and a method for using the same. Background Art

[0002] In a tubular luminescence test, the sample needs to be diluted first, and then mixed with a microsphere reagent (the microspheres are magnetic microspheres) in a reaction cup to form a sample reaction solution. At this time, the reaction substances in the sample will adhere to the microsphere carrier. Next, it enters the washing process of the reaction cup. The purpose of washing is to remove the liquid other than the microspheres. The existing washing process for the reaction cup in tubular luminescence mainly adopts the method of "magnet + steel needle", that is, using a magnet to attract the reaction substances (such as attracting them to the side of the reaction cup), and then using a steel needle to remove the liquid, or using a disposable tip to remove the liquid. Therefore, it is necessary to continuously inject cleaning liquid into the reaction cup for cleaning, and then remove it after cleaning. This is repeated many times. After washing is completed, another reagent is injected for chemiluminescence testing. The more reaction substances adhere to the magnetic microspheres, the brighter the light emitted, so as to obtain experimental data for the sample.

[0003] The existing reaction cup structures are all simple cup bodies, that is, the bottom of the cup body is a solid bottom, and the upper end of the cup body forms a cup mouth. When this kind of reaction cup is used in a tubular luminescence analyzer, it is necessary to add liquids such as samples, microsphere reagents (the microspheres are magnetic microspheres), and washing liquids (or cleaning liquids) into the reaction cup through a steel needle (which is part of the tubular luminescence analyzer) or a tip. The cleaning liquid and reaction liquid in the reaction cup also need to be aspirated and removed using a steel needle or a tip.

[0004] After the steel needle is used (to add or aspirate liquid) once, in order to prevent carrying contamination, it needs to be rinsed once. Only after rinsing can the steel needle be used for the next time. However, in actual operation, the rinsing of the steel needle is incomplete, and the steel needle still carries contamination.

[0005] In order to completely solve the problem that the steel needle still carries contamination after cleaning, disposable tips are now used instead. After the tip is used (to add or aspirate liquid) once, it has to be discarded. The loss of tips for a complex test is quite large.

[0006] In summary, when using a traditional reaction cup to conduct a test in a tubular luminescence analyzer, whether adding liquid into the reaction cup or removing liquid from the reaction cup, a steel needle or a tip is required, resulting in incomplete rinsing and easy carrying of contamination for the steel needle, and a large loss for disposable tips. Summary of the Invention

[0007] The technical problem to be solved by the present invention is: overcoming the deficiencies of the prior art, providing a reaction cup and its usage method, and solving the problems that when removing liquid from the existing reaction cup, a steel needle or a tip is required, resulting in incomplete flushing and easy contamination when using a steel needle to remove liquid, and large loss when using a tip to remove liquid.

[0008] The technical solution adopted by the present invention to solve its technical problems is:

[0009] First aspect:

[0010] Providing a reaction cup with a filter membrane, including

[0011] a cup body, an inlet is opened at the upper end of the cup body, an outlet is opened at the bottom of the cup body, and a filter membrane is arranged at the outlet;

[0012] When the outlet is at atmospheric pressure, the bottom membrane is suitable for supporting the sample reaction solution in the cup body;

[0013] When the outlet is under negative pressure, the sample reaction solution in the cup body is discharged through the outlet at the bottom of the cup, and the sample reaction solution is filtered through the filter membrane so that the microspheres in the sample reaction solution are retained in the cup body.

[0014] Further, a cup holder is arranged at the bottom of the body, the filter membrane is located between the cup body and the base, and a drain hole is opened on the cup holder, which is suitable for discharging liquid.

[0015] Further, an assembly step is formed at the bottom of the cup body, a step groove is formed in the cup holder, and a step fit is formed between the cup holder and the cup body.

[0016] Further, the micropore diameter of the filter membrane is less than 10 μm.

[0017] Further, the inlet is a stepped opening.

[0018] Second aspect:

[0019] Providing a usage method of the above reaction cup with a filter membrane, the bottom of the reaction cup is hermetically connected to a negative pressure liquid extraction device, the negative pressure liquid extraction device is hermetically communicated with the cup cavity, and the sample reaction solution in the reaction cup is extracted through the negative pressure liquid extraction device to retain the microspheres in the sample reaction solution in the cup body.

[0020] Further, the negative pressure liquid extraction device includes a vacuum pump and a liquid extraction pipe, the outlet of the liquid extraction pipe is connected to the vacuum pump, and the inlet of the liquid extraction pipe is suitable for forming a sealed fit with the base of the reaction cup.

[0021] The beneficial effects of the present invention are:

[0022] The present invention provides a reaction cup with a filter membrane and its usage method. The bottom of the reaction cup is provided with a filter membrane. When the reaction cup is in normal use under normal pressure, the filter membrane can support the reaction liquid in the reaction cup, enabling the reaction cup to be used normally. When it is necessary to remove the liquid in the reaction cup, only negative pressure adsorption is required at the bottom of the cup, and then the sample reaction liquid in the reaction cup can be removed. The filter membrane can screen the microspheres in the sample reaction liquid and retain them in the cup body.

[0023] When removing the liquid in the cup body of the reaction cup with a filter membrane, there is no need to rely on a steel needle and a tip head, completely avoiding the carry - over contamination caused by using a steel needle to remove the liquid, and also avoiding the consumption of a large number of tip heads during the experiment to remove the liquid.

[0024] The reaction cup with a filter membrane can remove the liquid from the bottom. When using the reaction cup with a filter membrane of the present invention for experiments, non - magnetic microsphere reagents can be selected, greatly improving the efficiency of removing the liquid in the reaction cup and the experimental efficiency. Brief Description of the Drawings

[0025] The present invention will be further described below with reference to the accompanying drawings.

[0026] Figure 1 is a three - dimensional view of the reaction cup;

[0027] Figure 2 is a semi - sectional view of the reaction cup;

[0028] Among them, 1 is the cup body, 2 is the filter membrane, 3 is the cup seat, and 31 is the drain hole. Detailed Embodiments

[0029] Now, the present invention will be further described with reference to specific embodiments. These drawings are all simplified schematic diagrams, which only illustrate the basic structure of the present invention in a schematic manner, so they only show the components related to the present invention.

[0030] Embodiment 1

[0031] As Figure 1 Figure 2 shown, a reaction cup with a filter membrane includes a cup body 1. An inlet is provided at the upper end of the cup body 1, and an outlet is provided at the bottom of the cup body 1. The outlet is provided with a filter membrane 2;

[0032] When the outlet is under normal pressure, the bottom membrane is suitable for supporting the sample reaction liquid in the cup body 1;

[0033] When the outlet is under negative pressure, the sample reaction liquid in the cup body 1 is discharged through the outlet at the bottom of the cup. The sample reaction liquid is filtered through the filter membrane 2, so that the microspheres in the sample reaction liquid are screened and retained in the cup body 1.

[0034] Specifically, as an alternative implementation in this embodiment, a cup holder 3 is provided at the bottom of the body. The filter membrane 2 is located between the cup body 1 and the base. A drain hole 31 is formed on the cup holder 3, which is adapted to drain the liquid.

[0035] Specifically, as an alternative implementation in this embodiment, an assembly step is formed at the bottom of the cup body 1, a step groove is formed inside the cup holder 3, and a step fit is formed between the cup holder 3 and the cup body 1.

[0036] In this embodiment, the step fit between the cup holder 3 and the cup body 1 can clamp the filter membrane 2 therebetween, preventing the filter membrane 2 from being touched and damaged by external substances, and playing a certain protective role for the filter membrane 2.

[0037] Specifically, as an alternative implementation in this embodiment, the micropore diameter of the filter membrane 2 is less than 10 μm. The micropore diameter of the filter membrane 2 is determined according to the particle sizes of different reaction substances.

[0038] The filter membrane 2 is a commercially available product, and the present invention does not involve improvements to the filter membrane 2.

[0039] Specifically, as an alternative implementation in this embodiment, the liquid inlet is a stepped opening.

[0040] In this embodiment, the filter membrane 2 and the cup body 1 are fixedly connected by hot melt welding, and the cup holder 3 and the cup body 1 are also fixedly connected by hot melt welding.

[0041] For the reaction cup of the present invention, a filter membrane 2 is provided at the bottom of the cup. By forming a negative pressure at the bottom of the cup, the liquid in the cup body 1 can be removed, realizing the filtration of the sample reaction liquid in the cup body 1, so that the removal of the sample reaction liquid in the reaction cup does not need to rely on a steel needle or a tip, reducing the loss of the tip and avoiding the contamination of the sample reaction liquid by the steel needle.

[0042] When using the reaction cup of the present invention in a tube type luminescence analyzer, when adding the sample, diluent and reagent into the reaction cup, it is still normally completed by relying on a steel needle or a tip. When the sample reaction liquid needs to be removed, as long as there is a negative pressure at the bottom of the reaction cup, the sample reaction liquid can be suction filtered out of the reaction cup, and the process of removing the sample reaction liquid from the reaction cup does not require the use of a steel needle and a tip.

[0043] Embodiment 2

[0044] A method for using the reaction cup with a filter membrane 2 in Embodiment 1. A negative pressure liquid extraction device is hermetically connected to the bottom of the reaction cup. The negative pressure liquid extraction device is hermetically communicated with the cup cavity. The sample reaction liquid in the reaction cup is extracted through the negative pressure liquid extraction device to screen the microspheres in the sample reaction liquid and leave them in the cup body.

[0045] Specifically, as an optional implementation manner in this embodiment, the negative-pressure liquid extraction device includes a vacuum pump and a liquid extraction pipe. The outlet of the liquid extraction pipe is connected to the vacuum pump, and the inlet of the liquid extraction pipe is adapted to form a sealed fit with the base of the reaction cup.

[0046] The liquid extraction pipe includes a flexible pipe and a rigid pipe. The flexible pipe connects the rigid pipe and the vacuum pump. The inlet of the rigid pipe is used for sealing cooperation with the cup holder 3 of the reaction cup. After the rigid pipe is in sealed cooperation with the cup holder 3, a negative pressure is provided by the vacuum pump, and then the sample reaction liquid in the reaction cup can be sucked away. When the sample reaction liquid is sucked away, it passes through the filtration of the filter membrane 2, so that the reaction substances in the sample reaction liquid are retained in the reaction cup, and then the reaction cup can participate in the next link.

[0047] For the usage method of the reaction cup of the present invention, by performing suction filtration on the reaction liquid in the cup at the bottom of the reaction cup, the removal of the sample reaction liquid does not need to rely on a steel needle or a tip, reducing the loss of the tip and avoiding the pollution of the sample reaction liquid by the steel needle.

[0048] Taking the above ideal embodiment based on the present invention as an inspiration, through the above description, relevant staff can completely make various changes and modifications without departing from the technical idea of this invention. The technical scope of this invention is not limited to the content in the specification, and its technical scope must be determined according to the scope of the claims.

Claims

1. A reaction cup with a filter membrane, characterized in that, including a cup body, with a liquid inlet formed at the upper end of the cup body, a liquid outlet formed at the bottom of the cup body, and a filter membrane provided at the liquid outlet; when the liquid outlet is at atmospheric pressure, the filter membrane is adapted to support the sample reaction liquid in the cup body; when the liquid outlet is under negative pressure, the sample reaction liquid in the cup body is discharged through the liquid outlet at the bottom of the cup, and the sample reaction liquid is filtered through the filter membrane so that the microspheres in the sample reaction liquid are retained in the cup body; a cup base is provided at the bottom of the cup body, the filter membrane is located between the cup body and the cup base, and a drain hole is formed on the cup base for discharging liquid; the micropore diameter of the filter membrane is less than 10 μm; an assembly step is formed at the bottom of the cup body, a step groove is formed in the cup base, a step fit is formed between the cup base and the cup body, the filter membrane is fixedly connected to the cup body by hot melt welding, and the cup base and the cup body are also fixedly connected by hot melt welding.

2. The filter membrane reaction cup according to claim 1, characterized in that, The liquid inlet is a stepped opening.

3. The usage method of the reaction cup with a filter membrane according to claim 1, characterized in that, The bottom of the reaction cup is hermetically connected to a negative pressure liquid extraction device, and the negative pressure liquid extraction device is hermetically communicated with the cup cavity. The sample reaction liquid in the reaction cup is extracted through the negative pressure liquid extraction device so that the microspheres in the sample reaction liquid are retained in the cup body.

4. The method for using a reaction cup with a filter membrane according to claim 3, wherein, The negative pressure liquid extraction device includes a vacuum pump and a liquid extraction pipe. The outlet of the liquid extraction pipe is connected to the vacuum pump, and the inlet of the liquid extraction pipe is adapted to form a sealed fit with the base of the reaction cup.

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