An anti-interference detection method and a sample analyzer

Abstract

Embodiments of the present application provide an anti-interference detection method and a sample analyzer. When the content of an interferent in a sample exceeds an anti-interference threshold, if anti-interference cannot be achieved by changing the wavelength, the sample to be measured is diluted, so as to achieve anti-interference measurement of the sample. The method provided by the embodiments of the present application comprises the following steps: obtaining the grade γ of an interferent in a sample; when a measurement item of the sample is measured by using multiple wavelengths, obtaining a main wavelength interferent grade threshold γ1 corresponding to a main wavelength matched with the measurement item, and a sub wavelength interferent grade threshold γ2 corresponding to a sub wavelength matched with the measurement item, wherein γ1<γ2; if γ≤γ1, the sample is measured by using the main wavelength; if γ1<γ≤γ2, anti-interference measurement of the sample is performed by using the sub wavelength; and if γ>γ2, the sample is diluted, and then anti-interference measurement of the diluted sample is performed by using the main wavelength or the sub wavelength.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of sample detection, and particularly relates to an anti-interference detection method and a sample analyzer. BACKGROUND

[0002] In the detection of coagulation items by optical method, collimated light is usually irradiated on the solution in the reaction cup during the reaction process, and the absorbance of the solution is obtained by analyzing the scattered or transmitted light, so as to obtain the clotting time or the concentration of the measured substance.

[0003] Generally, normal plasma is light yellow, transparent, and has very low absorbance, while jaundice, hemolysis and chylous samples with obvious absorption peaks and which can seriously affect the transmittance may affect the measurement and challenge the reliability of the optical method. Therefore, on the basis of pre-checking the sample, the anti-interference treatment should be performed on the interference sample, so as to obtain more accurate measurement results.

[0004] Since the three interference substances (hemolysis, jaundice and chylus) have different absorption spectra in the visible light band, the optical absorbance method is usually used to quantitatively or semi-quantitatively detect the interference substances. However, when the concentration of the detected interference substance exceeds the measurement threshold, how to realize anti-interference, that is, how to realize accurate measurement of the sample measurement item under the premise of exceeding the interference substance, is a problem to be solved at present. SUMMARY

[0005] The embodiments of the present application provide an anti-interference detection method and a sample analyzer, which are used to dilute the sample when the content of the interference substance in the sample exceeds the interference substance grade threshold, so as to realize anti-interference measurement of the sample.

[0006] The first aspect of the embodiments of the present application provides an anti-interference detection method, comprising:

[0007] obtaining the grade γ of the interference substance in the sample;

[0008] When a multi-wavelength is used to perform item measurement on the sample, the main wavelength interference substance grade threshold γ1 corresponding to the main wavelength matched with the measurement item and the secondary wavelength interference substance grade threshold γ2 corresponding to the secondary wavelength matched with the measurement item are obtained, wherein γ1<γ2;

[0009] If γ≤γ1, the main wavelength is used to measure the sample;

[0010] If γ1<γ≤γ2, the secondary wavelength is used to perform anti-interference measurement on the sample;

[0011] If γ>γ2, the sample is diluted, and then the main wavelength or the secondary wavelength is used to perform anti-interference measurement on the diluted sample.

[0012] Preferably, the step of performing anti-interference measurement on the diluted sample using the dominant wavelength or the secondary wavelength includes:

[0013] Obtain the interference level γ′ in the diluted sample;

[0014] If γ′≤γ1, then the dominant wavelength is used to perform anti-interference measurement on the sample;

[0015] If γ1 < γ′ ≤ γ2, then the sub-wavelength is used to perform anti-interference measurement on the sample.

[0016] Preferably, the dilution factor is a fixed value, or the dilution factor is proportional to the level γ of the interfering substance in the sample.

[0017] Preferably, the method further includes:

[0018] When performing project measurements on the sample using a single wavelength, obtain the interference level threshold γ3 corresponding to the single wavelength;

[0019] If γ≤γ3, then the single wavelength is used to perform the project measurement on the sample.

[0020] Preferably, the method further includes:

[0021] If γ > γ3, then the sample is diluted;

[0022] Obtain the interference level γ′ in the diluted sample;

[0023] If γ′≤γ3, then the single wavelength is used to perform anti-interference measurement on the sample.

[0024] Preferably, obtaining the level γ of interfering substances in the sample includes:

[0025] Collect the sample to be tested;

[0026] A portion of the sample is added to a second container to prepare a second mixture of the sample and diluent;

[0027] The second mixture is irradiated using a multi-wavelength light source;

[0028] Acquire the second optical detection signal of the second mixture;

[0029] Interference analysis is performed based on the second optical detection signal to obtain the interference level γ of the sample.

[0030] Preferably, the sample is plasma, and the interfering agent includes at least one of lipemia, hemolysis, and jaundice;

[0031] The step of irradiating the second mixture with a multi-wavelength light source includes:

[0032] The second mixture was irradiated with light sources of wavelengths of 405nm, 545nm, 660nm and 800nm.

[0033] Preferably, the step of performing interference analysis based on the second optical detection signal to obtain the interference level γ of the sample includes:

[0034] The absorbance values ​​of the lipemia in the second mixture at wavelengths of 660 nm and 800 nm were obtained respectively.

[0035] The lipid level in the plasma is calculated and analyzed according to the first formula, which is:

[0036]

[0037] Wherein, ΔAbsL represents the difference in absorbance of lipemia in the second mixture at wavelengths of 660nm and 800nm, C represents the lipemia grade coefficient, and L Index Indicates the level of lipid levels;

[0038] Wherein, γ is the L Index .

[0039] Preferably, the step of performing interference analysis based on the second optical detection signal to obtain the interference level γ of the sample further includes:

[0040] Obtain the absorbance values ​​of the hemolyzed solution in the second mixture at wavelengths of 545 nm and 660 nm.

[0041] The hemolysis level in the plasma was calculated and analyzed according to the second formula, which is:

[0042]

[0043] Wherein, ΔAbs H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, A represents the hemolysis grade coefficient, ΔAbs L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the correction coefficient for lipemia when calculating the hemolysis grade, and H Index Indicates the degree of hemolysis;

[0044] Wherein, γ includes L Index and the H Index .

[0045] Preferably, the step of performing interference analysis based on the second optical detection signal to obtain the interference level γ of the sample further includes:

[0046] Obtain the absorbance values ​​of jaundice in the second mixture at wavelengths of 405 nm and 660 nm.

[0047] The jaundice level in the plasma was calculated and analyzed according to the third formula, which is:

[0048]

[0049] Wherein, ΔAbs I represents the absorbance difference of jaundice in the second mixture at wavelengths of 405nm and 660nm, F represents the correction factor for hemolysis when calculating jaundice, ΔAbs H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, ΔAbs L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the correction factor for lipemia when calculating the hemolysis grade, G represents the correction factor for lipemia when calculating jaundice, D represents the jaundice grade coefficient, and I Index Indicates the jaundice level;

[0050] Wherein, γ includes L Index The H Index and the I Index .

[0051] A second aspect of this application provides a sample analyzer, comprising:

[0052] The first container is used to hold the first mixture prepared from a portion of the sample and the detection reagents;

[0053] The second container is used to hold the second mixture prepared from another portion of the sample and the diluent;

[0054] An optical device is used to irradiate the first mixture and the second mixture using a multi-wavelength light source;

[0055] A signal acquisition device is used to acquire a first optical detection signal of a first mixture and a second optical detection signal of a second mixture.

[0056] Processor, used for:

[0057] Based on the second optical detection signal, the level γ of the interfering substance in the sample is obtained;

[0058] When performing project measurements on the sample using multiple wavelengths, the main wavelength interference level threshold γ1 corresponding to the main wavelength matching the measurement project and the secondary wavelength interference level threshold γ2 corresponding to the secondary wavelength matching the measurement project are obtained, where γ1 < γ2.

[0059] If γ≤γ1, then the dominant wavelength is used to perform anti-interference measurement on the sample;

[0060] If γ1 < γ ≤ γ2, then the sub-wavelength is used to perform anti-interference measurement on the sample;

[0061] If γ > γ2, the sample is diluted, and then anti-interference measurement is performed on the diluted sample using the main wavelength or the secondary wavelength.

[0062] Preferably, the processor is specifically used for:

[0063] Obtain the interference level γ′ in the diluted sample;

[0064] If γ′≤γ1, then the dominant wavelength is used to perform anti-interference measurement on the sample;

[0065] If γ1 < γ′ ≤ γ2, then the sub-wavelength is used to perform anti-interference measurement on the sample.

[0066] Preferably, the dilution factor is a fixed value, or the dilution factor is proportional to the level γ of the interfering substance in the sample.

[0067] Preferably, the processor is further configured to:

[0068] When performing project measurements on the sample using a single wavelength, obtain the interference level threshold γ3 corresponding to the single wavelength;

[0069] If γ≤γ3, then the single wavelength is used to perform the project measurement on the sample.

[0070] Preferably, the processor is further configured to:

[0071] If γ > γ3, then the sample is diluted;

[0072] Obtain the interference level γ′ in the diluted sample;

[0073] If γ′≤γ3, then the single wavelength is used to perform anti-interference measurement on the sample.

[0074] Preferably, the processor is specifically used for:

[0075] Interference analysis is performed based on the second optical detection signal to obtain the interference level γ of the sample.

[0076] Preferably, the sample is plasma, and the interfering agent includes at least one of lipemia, hemolysis, and jaundice;

[0077] The processor is specifically used for:

[0078] The second mixture was irradiated with light sources of wavelengths of 405nm, 545nm, 660nm and 800nm.

[0079] Preferably, the processor is specifically used for:

[0080] The absorbance values ​​of the lipemia in the second mixture at wavelengths of 660 nm and 800 nm were obtained respectively.

[0081] The lipid level in the plasma is calculated and analyzed according to the first formula, which is:

[0082]

[0083] Where ΔAbsL represents the difference in absorbance of lipemia at wavelengths of 660nm and 800nm, C represents the lipemia grade coefficient, and L Index Indicates the level of lipid levels;

[0084] Wherein, γ is the L Index .

[0085] Preferably, the processor is further configured to:

[0086] Obtain the absorbance values ​​of the hemolyzed solution in the second mixture at wavelengths of 545 nm and 660 nm.

[0087] The hemolysis level in the plasma was calculated and analyzed according to the second formula, which is:

[0088]

[0089] Where ΔAbs H represents the absorbance difference of hemolysis at wavelengths of 545 nm and 660 nm, A represents the hemolysis grade coefficient, ΔAbs L represents the absorbance difference of lipemia at wavelengths of 660 nm and 800 nm, E represents the correction coefficient for lipemia when calculating the hemolysis grade, and H Index Indicates the degree of hemolysis;

[0090] Wherein, γ includes L Index and the H Index .

[0091] Preferably, the processor is further configured to:

[0092] Obtain the absorbance values ​​of jaundice in the second mixture at wavelengths of 405 nm and 660 nm.

[0093] The jaundice level in the plasma was calculated and analyzed according to the third formula, which is:

[0094]

[0095] Wherein, ΔAbs I represents the absorbance difference of jaundice in the second mixture at wavelengths of 405nm and 660nm, F represents the correction factor for hemolysis when calculating jaundice, ΔAbs H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, ΔAbs L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the correction factor for lipemia when calculating the hemolysis grade, G represents the correction factor for lipemia when calculating jaundice, D represents the jaundice grade coefficient, and I Index Indicates the jaundice level;

[0096] Wherein, γ includes L Index The H Index and the I Index .

[0097] Preferably, the sample analyzer is a coagulation analyzer.

[0098] This application also provides a computer-readable storage medium storing a computer program thereon, which, when executed by a processor, is used to implement the anti-interference detection method provided in the first aspect of this application.

[0099] As can be seen from the above technical solutions, the embodiments of this application have the following advantages:

[0100] In this embodiment, the level γ of interfering substances in the sample is obtained. When multiple wavelengths are used to perform a measurement on the sample, the main wavelength interfering substance level threshold γ1 corresponding to the main wavelength matching the measurement item and the secondary wavelength interfering substance level threshold γ2 corresponding to the secondary wavelength matching the measurement item are obtained, where γ1 < γ2. If γ ≤ γ1, the main wavelength is used to measure the sample; if γ1 < γ ≤ γ2, the secondary wavelength is used to perform anti-interference measurement on the sample; if γ > γ2, the sample is diluted, and then the diluted sample is subjected to anti-interference measurement using either the main wavelength or the secondary wavelength. In this embodiment, if the interfering substance content in the sample exceeds the interfering substance level threshold, and anti-interference cannot be achieved by wavelength switching, the sample to be tested is diluted to achieve anti-interference measurement of the sample. Attached Figure Description

[0101] Figure 1 This is a schematic diagram of the sample analyzer in one embodiment of this application;

[0102] Figure 2 This is another schematic diagram of the sample analyzer in the embodiments of this application;

[0103] Figure 3This is another schematic diagram of the sample analyzer in the embodiments of this application;

[0104] Figure 4 This is a schematic diagram of one embodiment of the anti-interference detection method in this application;

[0105] Figure 5 Examples of embodiments in this application Figure 4 Detailed steps of step 405 in the embodiment;

[0106] Figure 6 This is a schematic diagram of another embodiment of the anti-interference detection method in this application;

[0107] Figure 7 Examples of embodiments in this application Figure 4 Detailed steps of step 401 in the embodiment;

[0108] Figure 8 Examples of embodiments in this application Figure 7 Detailed steps of step 705 in the embodiment;

[0109] Figure 9 These are the absorbance spectra of three different interfering substances in the embodiments of this application;

[0110] Figure 10 This is a schematic diagram of one embodiment of the sample analyzer in this application. Detailed Implementation

[0111] This application provides an anti-interference detection method and a sample analyzer, which is used to dilute the sample to be tested when the content of interfering substances in the sample exceeds the interfering substance level threshold. If the anti-interference method cannot be achieved by cutting the wavelength, the sample is diluted to achieve anti-interference measurement of the sample.

[0112] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present application, and not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort should fall within the scope of protection of the present application.

[0113] The terms "first," "second," "third," "fourth," etc., used in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that the embodiments described herein can be implemented in a sequence other than that illustrated or described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover a non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0114] Before detailing this application, the structure of the sample analyzer will be described first. Please refer to [link / reference]. Figure 1 In one embodiment, the sample analyzer may include a sample component 10, a reagent component 20, a measurement component 30, a memory 40, and a processor 50; in some embodiments, please refer to Figure 2 The sample analyzer may also include a display and operation unit 60; this will be described in detail below.

[0115] The sample component 10 is used to hold the sample in the test solution and, after aspirating the sample, provides it to the measurement component 30. Please refer to... Figure 3 In some embodiments, sample component 10 may include sample carrying component 11 and sample dispensing mechanism 12. Sample carrying component 11 is used to carry samples. In some examples, sample carrying component 11 may include sample delivery module (SDM) and front-end track; in other examples—for example Figure 3 In such an example, the sample carrier 11 could also be a sample tray, which includes multiple sample positions for placing sample tubes. By rotating its tray structure, the sample can be moved to the appropriate position, such as the position for the sample dispensing mechanism 12 to pick up the sample. The sample dispensing mechanism 12 is used to pick up the sample and dispense it into the reaction cup to be added. For example, the sample dispensing mechanism 12 may include a sample needle, which is driven by a two-dimensional or three-dimensional mechanism to move in two-dimensional or three-dimensional space, thereby moving the sample needle to pick up the sample carried by the sample carrier 11, and to move to the reaction cup to be added, and dispense the sample into the reaction cup.

[0116] The reagent component 20 is used to carry the reagent in the test solution and, after aspirating the reagent, provides it to the measuring component 30. In some embodiments, the reagent component 20 may include a reagent carrying component 13 and a reagent dispensing mechanism 14. The reagent carrying component 13 is used to carry the reagent. In one embodiment, the reagent carrying component 13 may be a reagent tray, which is arranged in a disc-shaped structure and has multiple positions for carrying reagent containers. The reagent carrying component 13 is rotatable and drives the reagent container it carries to rotate, for rotating the reagent container to a specific position, such as the position where the reagent dispensing mechanism 14 aspirates the reagent. The number of reagent carrying components 13 may be one or more. The reagent dispensing mechanism 14 is used to aspirate the reagent and discharge it into a reaction cup to which the reagent is to be added. In one embodiment, the reagent dispensing mechanism 14 may include a reagent needle, which is driven by a two-dimensional or three-dimensional mechanism to move in two-dimensional or three-dimensional space, so that the reagent needle can move to aspirate the reagent carried by the reagent carrying component 13, move to the reaction cup to which the reagent is to be added, and discharge the reagent into the reaction cup.

[0117] The measuring component 30 is used to perform tests on the sample to obtain test data. In some embodiments, the measuring component 30 may include a reaction component 15 and a photometric component 16. Specifically, in the embodiments of this application, the reaction component is a first container and a second container, wherein the first container is used to hold a first mixture prepared from a portion of the sample and a detection reagent; the second container is used to hold a second mixture prepared from another portion of the sample and a diluent. The photometric component 16 is used to perform photometric measurements on the first and second mixtures to obtain the sample's reaction data, such as detecting the luminescence intensity of the reaction solution to be tested and obtaining the concentration of the analyte in the sample by querying a calibration curve. Specifically, in this embodiment, the photometric component 16 includes a multi-wavelength light source, a spectrometer, an optical processing device, and a signal acquisition device. In some embodiments, the photometric component 16 may also be separately disposed outside the reaction component 15.

[0118] based on Figures 1 to 3 The sample analyzer described in the embodiments is described below. The anti-interference detection method in the embodiments of this application is described in detail below. Please refer to [link / reference]. Figure 4 One embodiment of an anti-interference detection method in this application includes:

[0119] 401. Obtain the level γ of interfering substances in the sample;

[0120] In order to achieve accurate measurement of sample measurement items, it is generally required that the content of interfering substances in the sample is less than the interfering substance threshold when performing item testing on the sample. Otherwise, the photometric component will not be able to accurately measure the absorbance value during item testing, thus affecting the normal item testing of the sample.

[0121] Therefore, in this embodiment, when performing project testing on the sample, it is necessary to obtain the content of interfering substances in the sample (i.e., the concentration or level of interfering substances). Among them, the concentration and level of interfering substances are two ways to describe the content of interfering substances, and they are in one-to-one correspondence. For ease of description, this embodiment uses the level γ of interfering substances to describe the content of interfering substances.

[0122] 402. When performing project measurements on the sample using multiple wavelengths, obtain the main wavelength interference level threshold γ1 corresponding to the main wavelength matching the measurement project, and the secondary wavelength interference level threshold γ2 corresponding to the secondary wavelength matching the measurement project, wherein γ1 < γ2.

[0123] When performing multi-wavelength measurements on a sample (such as immunoturbidimetric assays), each measurement typically has a dominant wavelength and a secondary wavelength. The dominant and secondary wavelengths used for the measurement each have their own corresponding anti-interference thresholds γ1 and γ2, where γ1 < γ2. In this embodiment, when performing measurements on the sample using multiple wavelengths, it is necessary to obtain the dominant wavelength interference level threshold γ1 corresponding to the dominant wavelength matching the measurement, and the secondary wavelength interference level threshold γ2 corresponding to the secondary wavelength matching the measurement.

[0124] When performing tests on samples, the concentration or level of interfering substances must be lower than the interfering substance level threshold corresponding to the dominant wavelength or sub-wavelength, respectively. Otherwise, accurate measurement of the measured items cannot be achieved. The dominant wavelength interfering substance level threshold refers to the maximum permissible concentration or level of interfering substances when performing measurement analysis on the sample using the dominant wavelength; the sub-wavelength interfering substance level threshold refers to the maximum permissible concentration or level of interfering substances when performing measurement analysis on the sample using the sub-wavelength.

[0125] In this embodiment, the selection of the primary and secondary wavelengths is mainly determined by the reagent requirements during the sample measurement process. When performing measurement on the sample, the primary wavelength is required to characterize the reaction process between the sample and the reagent as much as possible, while also ensuring that the signal intensity (i.e., the intensity of transmitted light) collected by the photometer during the entire test process meets the requirements of the measurement.

[0126] 403. If γ≤γ1, then the sample shall be measured using the dominant wavelength;

[0127] If the interference level γ in the sample is less than or equal to the interference level threshold γ1 of the main wavelength, then the main wavelength can be used to achieve accurate measurement of the sample measurement items.

[0128] 404. If γ1 < γ ≤ γ2, then the sub-wavelength is used to perform anti-interference measurement on the sample;

[0129] If the interference level γ in the sample is greater than the main wavelength interference level threshold γ1, but not greater than the secondary wavelength interference level threshold γ2, then the wavelength cutting method can be used, that is, the secondary wavelength can be used to achieve anti-interference measurement of the sample measurement items.

[0130] 405. If γ > γ2, the sample is diluted, and then anti-interference measurement is performed on the diluted sample using the main wavelength or the secondary wavelength.

[0131] If the level of interference in the sample is greater than the threshold for the level of interference in the secondary wavelength, that is, γ>γ2, the sample can be diluted until the level of interference in the sample is less than the anti-interference level threshold of the primary wavelength or the secondary wavelength. Then, anti-interference measurement is performed on the diluted sample using the primary wavelength or the secondary wavelength.

[0132] Specifically, anti-interference testing can be performed on diluted samples in the following two ways; please refer to [link / reference]. Figure 5 As described in the examples.

[0133] In this embodiment, the level γ of interfering substances in the sample is obtained. When multiple wavelengths are used to perform a measurement on the sample, the main wavelength interfering substance level threshold γ1 corresponding to the main wavelength matching the measurement item and the secondary wavelength interfering substance level threshold γ2 corresponding to the secondary wavelength matching the measurement item are obtained, where γ1 < γ2. If γ ≤ γ1, the main wavelength is used to measure the sample; if γ1 < γ ≤ γ2, the secondary wavelength is used to perform anti-interference measurement on the sample; if γ > γ2, the sample is diluted, and then the diluted sample is subjected to anti-interference measurement using either the main wavelength or the secondary wavelength. In this embodiment, if the interfering substance content in the sample exceeds the interfering substance level threshold, and anti-interference cannot be achieved by wavelength switching, the sample to be tested is diluted to achieve anti-interference measurement of the sample.

[0134] based on Figure 4 In the aforementioned embodiment, when the interference level in the sample exceeds the sub-wavelength interference level threshold (i.e., γ > γ2), accurate measurement of the sample's measurement items cannot be achieved by the wavelength-switching method. Therefore, the following describes in detail two methods for performing anti-interference detection on diluted samples; please refer to [link to relevant documentation]. Figure 5 , Figure 5 for Figure 4 Detailed steps of step 405 in the embodiment:

[0135] 501. Obtain the interference level γ′ in the diluted sample. If γ′≤γ1, then use the dominant wavelength to perform anti-interference measurement on the sample.

[0136] After diluting the sample, the level of interference γ′ in the diluted sample can be obtained. If γ′≤γ1, the main wavelength is used to perform anti-interference measurement on the sample.

[0137] Specifically, the dilution factor of a sample is proportional to the level γ of the interfering substance in the sample. It can be calculated using the first formula, or the sample can be diluted by a fixed factor, such as diluting the sample to 3 or 4 times its original value. However, it is necessary to ensure that the level γ′ of the interfering substance in the diluted sample is not greater than the interfering substance level threshold γ1 of the main wavelength.

[0138] The first formula is:

[0139] A represents the dilution factor, γ represents the level of interfering substances in the sample before dilution, and γ1 represents the threshold for the level of interfering substances at the main wavelength.

[0140] Furthermore, after diluting the sample, the level of interference γ′ in the diluted sample can be obtained by measuring with an optical measurement device. There are no specific restrictions on the method of obtaining the level of interference γ′ in the diluted sample.

[0141] 502. Obtain the interference level γ′ in the diluted sample. If γ1 < γ′ ≤ γ2, then use the subwavelength to perform anti-interference measurement on the sample.

[0142] After diluting the sample, the level of interference γ′ in the diluted sample can be obtained. If γ1<γ′≤γ2, the subwavelength is used to perform anti-interference measurement on the sample.

[0143] Specifically, the dilution factor of a sample is directly proportional to the level γ of the interfering substance in the sample. This can be calculated using the second formula, or the sample can be diluted by a fixed factor, such as diluting it to 3 or 4 times its original value. However, it is necessary to ensure that the level γ′ of the interfering substance in the diluted sample is not greater than the interfering substance level threshold γ2 of the subwavelength.

[0144] The second formula is:

[0145] A represents the dilution factor, γ represents the level of interfering substances in the sample before dilution, and γ2 represents the threshold for the level of interfering substances at the subwavelength.

[0146] Furthermore, after diluting the sample, the level of interference γ′ in the diluted sample can be obtained by measuring with an optical measurement device. There are no specific restrictions on the method of obtaining the level of interference γ′ in the diluted sample.

[0147] In this embodiment of the application, when the level of interference in the sample is greater than the anti-interference level threshold of the subwavelength, the anti-interference method for performing project detection on the diluted sample is described in detail, which improves the accuracy of performing project detection on the sample.

[0148] The above embodiments describe in detail the anti-interference detection method when the sample is tested using multiple wavelengths. The following describes the anti-interference detection method for measurement items that cannot be measured by wavelength cutting (i.e., single wavelength), such as the anti-interference detection method of the chromogenic substrate method. In this case, the anti-interference of a single wavelength only has a single threshold, namely the interference level threshold of the main wavelength.

[0149] For details, please refer to Figure 6 Another embodiment of the anti-interference detection method in this application includes:

[0150] 601. When performing project measurements on the sample using a single wavelength, obtain the interference level threshold γ3 corresponding to the single wavelength;

[0151] When performing item detection on a sample using a single wavelength, after obtaining the interference level γ in the sample, in order to achieve accurate measurement of the sample measurement item, it is also necessary to obtain the interference level threshold γ3 corresponding to the single wavelength, and perform the following steps based on the comparison result between γ and γ3.

[0152] 602. If γ≤γ3, then the single wavelength is used to perform the project measurement on the sample;

[0153] If the level of interference in the sample is not greater than the threshold value of interference level corresponding to a single wavelength, i.e., γ≤γ3, then a single wavelength is used to perform item detection on the sample in order to achieve accurate measurement of the sample measurement items.

[0154] 603. If γ > γ3, then the sample shall be diluted;

[0155] If the interference level in the sample is greater than the interference level threshold corresponding to a single wavelength (i.e., γ>γ3), in order to achieve accurate measurement of the sample measurement items, the sample can be diluted until the interference level in the diluted sample is no greater than the interference level threshold corresponding to a single wavelength.

[0156] 604. Obtain the interference level γ′ in the diluted sample;

[0157] Specifically, after diluting the sample, the level of interference γ′ in the diluted sample can be obtained, and step 605 is performed based on the comparison between γ′ and γ3.

[0158] For details on how to dilute the interfering substance level in the sample from γ to γ′, please refer to [link to relevant documentation]. Figure 5As described in the examples, they will not be repeated here.

[0159] 605. If γ′≤γ3, then the single wavelength is used to perform anti-interference measurement on the sample.

[0160] When the interference level in the diluted sample is not greater than the interference level threshold corresponding to a single wavelength, i.e., γ′≤γ3, a single wavelength can be used to perform anti-interference measurement on the sample to achieve accurate measurement of the sample measurement items.

[0161] In this embodiment, the anti-interference detection method for single-wavelength measurement items is described in detail, which improves the accuracy of single-wavelength item detection on samples.

[0162] based on Figures 4 to 6 The embodiments described below are described in detail. Figure 4 For details of step 401 in the embodiment, please refer to [link / reference]. Figure 7 , Figure 7 for Figure 4 Detailed steps of step 401 in the embodiment:

[0163] 701. Take the sample to be tested;

[0164] In this application, when measuring interfering substances in a sample, the sample analyzer first picks up the sample to be tested and then performs step 702.

[0165] 702. Add a portion of the sample to the second container to prepare a second mixture of the sample and diluent;

[0166] When measuring interfering substances in the sample, embodiments of this application add a portion of the sample to a second container to prepare a second mixture of the sample and diluent.

[0167] 703. Irradiate the second mixture using a multi-wavelength light source;

[0168] After obtaining the second mixture, the second mixture is irradiated with a multi-wavelength light source in the optical measurement component, and step 704 is executed.

[0169] 704. Acquire the second optical detection signal of the second mixture;

[0170] After irradiating the second mixture with a multi-wavelength light source, the second optical detection signal of the second mixture is acquired by the signal acquisition device in the optical measurement component, and step 705 is executed based on the second optical detection signal.

[0171] 705. Perform interference analysis based on the second optical detection signal to obtain the interference level γ of the sample.

[0172] After obtaining the second optical detection signal, the level of interference in the sample is analyzed based on the second optical detection signal to obtain the level γ of the interference.

[0173] Specifically, the process of analyzing and obtaining the interference level γ based on the second optical detection signal is described in detail in the following embodiments, and will not be repeated here.

[0174] based on Figure 7 In the embodiments described above, when the sample is plasma, the interfering substances include at least one of lipemia, hemolysis, and jaundice, and the multi-wavelength light sources are light sources with wavelengths of 405nm, 545nm, 660nm, and 800nm ​​respectively, the following description continues. Figure 7 For step 705 in the embodiment, please refer to... Figure 8 , Figure 8 for Figure 7 Detailed steps of step 705 in the embodiment:

[0175] 801. Obtain the absorbance values ​​of the lipemia in the second mixture at wavelengths of 660 nm and 800 nm, respectively;

[0176] Because this embodiment measures the interfering substance by using optical signal acquisition on the diluted sample, it is necessary to obtain the absorbance values ​​of the interfering substance at different wavelengths in the second mixture when calculating the concentration of the interfering substance in the second mixture.

[0177] It should be noted that in the embodiments of this application, the content of the interfering substance is described by the level of the interfering substance. The level of the interfering substance and the concentration of the interfering substance are in one-to-one correspondence, and the correspondence is described in the prior art, so it will not be repeated here.

[0178] For ease of explanation, Figure 9 The absorbance spectra of three different interfering substances are given. Since lipemia (chylous interference) has obvious absorption at 660 nm, while hemoglobin and bilirubin do not show absorption at 660 nm, based on the strong specificity of the absorption spectrum of lipemia, the difference in absorbance at 660 nm and 800 nm is used to determine whether lipemia is present. Therefore, it is necessary to obtain the absorbance values ​​of lipemia at wavelengths of 660 nm and 800 nm respectively.

[0179] 802. The lipid level in the plasma is calculated and analyzed according to the first formula, which is:

[0180]

[0181] Where ΔAbs L represents the difference in absorbance of lipemia in the second mixture at wavelengths of 660 nm and 800 nm (relative to the blank channel), C represents the lipemia grade coefficient, and L Index This indicates the level of lipid levels.

[0182] After obtaining the absorbance values ​​of lipemia in the second mixture at wavelengths of 660 nm and 800 nm, the lipemia level in the plasma can be calculated and analyzed according to the first formula.

[0183] 803. Obtain the absorbance values ​​of the hemolyzed solution in the second mixture at wavelengths of 545 nm and 660 nm;

[0184] Because hemolysis (hemoglobin interference) has strong absorption at both 405 and 545 nm, but has strong specificity at 545 nm in the green light band, and jaundice does not show obvious absorption in the green light band, the absorbance of hemolysis at 545 nm in the green light band only includes two cases: lipemia and hemolysis. Subtracting the absorbance of lipemia can yield the absorbance of hemolysis. Therefore, the absorbance values ​​of hemolysis at wavelengths of 545 nm and 660 nm can be obtained separately to calculate the degree of hemolysis.

[0185] 804. The hemolysis level in the plasma is calculated and analyzed according to the second formula, which is:

[0186]

[0187] Wherein, ΔAbs H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm (relative to the blank channel), A represents the hemolysis grade coefficient, ΔAbs L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm ​​(relative to the blank channel), E represents the correction coefficient for lipemia when calculating the hemolysis grade, and H Index Indicates the degree of hemolysis.

[0188] After obtaining the absorbance values ​​of hemolysis in the second mixture at wavelengths of 545 nm and 660 nm, the hemolysis level in the plasma can be calculated and analyzed according to the second formula.

[0189] 805. Obtain the absorbance values ​​of jaundice in the second mixture at wavelengths of 405 nm and 660 nm;

[0190] Because jaundice (bilirubin interference) has strong absorption around 440nm, this embodiment uses a wavelength of 405nm for testing. Figure 9 As shown in the absorption spectrum, all three types of interference have strong absorption in the 405nm band (jaundice has the weakest anti-interference ability), while the absorption of jaundice is almost zero at 660nm. Therefore, the jaundice level can be calculated by the difference in absorbance at wavelengths of 405nm and 660nm. When calculating the jaundice level, the interference of hemolysis and lipemia needs to be subtracted.

[0191] 806. The jaundice level in the plasma is calculated and analyzed according to the third formula, which is:

[0192]

[0193] Wherein, ΔAbs I represents the absorbance difference of jaundice in the second mixture at wavelengths of 405 nm and 660 nm, F represents the correction factor for hemolysis when calculating jaundice, ΔAbs H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545 nm and 660 nm, ΔAbs L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660 nm and 800 nm, E represents the correction factor for lipemia when calculating the hemolysis grade, G represents the correction factor for lipemia when calculating jaundice, D represents the jaundice grade coefficient, and I Index Indicates the level of jaundice.

[0194] After obtaining the absorbance values ​​of jaundice at wavelengths of 405nm and 660nm respectively, the jaundice level in plasma can be calculated and analyzed according to the third formula.

[0195] It should be noted that when the interfering substances in the plasma only include lipemia, then the interfering substance grade γ is the lipemia grade L. Index When the interfering factors in the plasma include lipemia, hemolysis, and jaundice, the interfering factor grade γ includes lipemia grade L. Index Hemolysis grade H Index Jaundice grade I Index .

[0196] In this embodiment, the process of performing interference analysis based on the second optical detection signal to obtain the interference level γ of the sample is described in detail, which improves the feasibility of this embodiment.

[0197] The anti-interference detection method in this application has been described in detail above. The sample analyzer in this application will now be described in detail below. Please refer to [link / reference]. Figure 10 One embodiment of the sample analyzer in this application includes:

[0198] The first container 1001 is used to hold the first mixture prepared from a portion of the sample and the detection reagents;

[0199] The second container 1002 is used to hold a second mixture prepared from another portion of the sample and the diluent;

[0200] Optical device 1003 is used to irradiate the first mixture and the second mixture using multi-wavelength light source 10031 and optical processing device 10032;

[0201] Signal acquisition device 1004 is used to acquire the first optical detection signal of the first mixture and the second optical detection signal of the second mixture;

[0202] Processor 1005, used for:

[0203] Based on the second optical detection signal, the level γ of the interfering substance in the sample is obtained;

[0204] When performing project measurements on the sample using multiple wavelengths, the main wavelength interference level threshold γ1 corresponding to the main wavelength matching the measurement project and the secondary wavelength interference level threshold γ2 corresponding to the secondary wavelength matching the measurement project are obtained, where γ1 < γ2.

[0205] If γ≤γ1, then the dominant wavelength is used to perform anti-interference measurement on the sample;

[0206] If γ1 < γ ≤ γ2, then the sub-wavelength is used to perform anti-interference measurement on the sample;

[0207] If γ > γ2, the sample is diluted, and then anti-interference measurement is performed on the diluted sample using the main wavelength or the secondary wavelength.

[0208] Preferably, the processor 1005 is specifically used for:

[0209] Obtain the interference level γ′ in the diluted sample;

[0210] If γ′≤γ1, then the dominant wavelength is used to perform anti-interference measurement on the sample;

[0211] If γ1 < γ′ ≤ γ2, then the sub-wavelength is used to perform anti-interference measurement on the sample.

[0212] Preferably, the dilution factor is a fixed value, or the dilution factor is proportional to the level γ of the interfering substance in the sample.

[0213] Preferably, the processor 1005 is further configured to:

[0214] When performing project measurements on the sample using a single wavelength, obtain the interference level threshold γ3 corresponding to the single wavelength;

[0215] If γ≤γ3, then the single wavelength is used to perform the project measurement on the sample.

[0216] Preferably, the processor 1005 is further configured to:

[0217] If γ > γ3, then the sample is diluted;

[0218] Obtain the interference level γ′ in the diluted sample;

[0219] If γ′≤γ3, then the single wavelength is used to perform anti-interference measurement on the sample.

[0220] Preferably, the processor 1005 is specifically used for:

[0221] Interference analysis is performed based on the second optical detection signal to obtain the interference level γ of the sample.

[0222] Preferably, the sample is plasma, and the interfering agent includes at least one of lipemia, hemolysis, and jaundice;

[0223] The processor 1005 is specifically used for:

[0224] The second mixture was irradiated with light sources of wavelengths of 405nm, 545nm, 660nm and 800nm.

[0225] Preferably, the processor 1005 is specifically used for:

[0226] The absorbance values ​​of the lipemia in the second mixture at wavelengths of 660 nm and 800 nm were obtained respectively.

[0227] The lipid level in the plasma is calculated and analyzed according to the first formula, which is:

[0228]

[0229] Where ΔAbsL represents the difference in absorbance of lipemia at wavelengths of 660nm and 800nm, C represents the lipemia grade coefficient, and L Index Indicates the level of lipid levels;

[0230] Wherein, γ is the L Index .

[0231] Preferably, the processor 1005 is further configured to:

[0232] Obtain the absorbance values ​​of the hemolyzed solution in the second mixture at wavelengths of 545 nm and 660 nm.

[0233] The hemolysis level in the plasma was calculated and analyzed according to the second formula, which is:

[0234]

[0235] Where ΔAbs H represents the absorbance difference of hemolysis at wavelengths of 545 nm and 660 nm, A represents the hemolysis grade coefficient, ΔAbs L represents the absorbance difference of lipemia at wavelengths of 660 nm and 800 nm, E represents the correction coefficient for lipemia when calculating the hemolysis grade, and H Index Indicates the degree of hemolysis;

[0236] Wherein, γ includes L Index and the H Index .

[0237] Preferably, the processor 1005 is further configured to:

[0238] Obtain the absorbance values ​​of jaundice in the second mixture at wavelengths of 405 nm and 660 nm.

[0239] The jaundice level in the plasma was calculated and analyzed according to the third formula, which is:

[0240]

[0241] Wherein, ΔAbs I represents the absorbance difference of jaundice in the second mixture at wavelengths of 405nm and 660nm, F represents the correction factor for hemolysis when calculating jaundice, ΔAbs H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, ΔAbs L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the correction factor for lipemia when calculating the hemolysis grade, G represents the correction factor for lipemia when calculating jaundice, D represents the jaundice grade coefficient, and I Index Indicates the jaundice level;

[0242] Wherein, γ includes L Index The H Index and the I Index .

[0243] It should be noted that the working principles of the above devices are the same as those of... Figures 4 to 9 The examples described are similar and will not be repeated here.

[0244] In this embodiment, the processor 1005 obtains the level γ of interfering substances in the sample. When performing a measurement on the sample using multiple wavelengths, the processor obtains the main wavelength interfering substance level threshold γ1 corresponding to the main wavelength matching the measurement item, and the secondary wavelength interfering substance level threshold γ2 corresponding to the secondary wavelength matching the measurement item, wherein γ1 < γ2. If γ ≤ γ1, the main wavelength is used to measure the sample; if γ1 < γ ≤ γ2, the secondary wavelength is used to perform anti-interference measurement on the sample; if γ > γ2, the sample is diluted, and then the diluted sample is subjected to anti-interference measurement using either the main wavelength or the secondary wavelength. In this embodiment, if the interfering substance content in the sample exceeds the interfering substance level threshold, and anti-interference cannot be achieved by wavelength switching, the sample to be tested is diluted to achieve anti-interference measurement of the sample.

[0245] It should be noted that when the sample analyzer performs coagulation tests, it is a coagulation analyzer. The structure and working principle of the coagulation analyzer can be found in [reference needed]. Figures 4 to 10 The embodiments described herein will not be repeated here.

[0246] This application embodiment also provides a computer-readable storage medium for implementing the functions of a sample analyzer. The medium stores a computer program, which, when executed by a processor, allows the processor to perform the following steps:

[0247] Obtain the level γ of interfering substances in the sample;

[0248] When performing project measurements on the sample using multiple wavelengths, the main wavelength interference level threshold γ1 corresponding to the main wavelength matching the measurement project and the secondary wavelength interference level threshold γ2 corresponding to the secondary wavelength matching the measurement project are obtained, where γ1 < γ2.

[0249] If γ≤γ1, then the sample is measured using the dominant wavelength;

[0250] If γ1 < γ ≤ γ2, then the sub-wavelength is used to perform anti-interference measurement on the sample;

[0251] If γ > γ2, the sample is diluted, and then anti-interference measurement is performed on the diluted sample using the main wavelength or the secondary wavelength.

[0252] In some embodiments of this application, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may specifically perform the following steps:

[0253] Obtain the interference level γ′ in the diluted sample;

[0254] If γ′≤γ1, then the dominant wavelength is used to perform anti-interference measurement on the sample;

[0255] If γ1 < γ′ ≤ γ2, then the sub-wavelength is used to perform anti-interference measurement on the sample.

[0256] In some embodiments of this application, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may specifically perform the following steps:

[0257] The dilution factor can be set to a fixed value, or the dilution factor can be set to be proportional to the level γ of the interfering substance in the sample.

[0258] In some embodiments of this application, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may specifically perform the following steps:

[0259] When performing project measurements on the sample using a single wavelength, obtain the interference level threshold γ3 corresponding to the single wavelength;

[0260] If γ≤γ3, then the single wavelength is used to perform the project measurement on the sample.

[0261] In some embodiments of this application, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may specifically perform the following steps:

[0262] If γ > γ3, then the sample is diluted;

[0263] Obtain the interference level γ′ in the diluted sample;

[0264] If γ′≤γ3, then the single wavelength is used to perform anti-interference measurement on the sample.

[0265] In some embodiments of this application, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may specifically perform the following steps:

[0266] Collect the sample to be tested;

[0267] A portion of the sample is added to a second container to prepare a second mixture of the sample and diluent;

[0268] The second mixture is irradiated using a multi-wavelength light source;

[0269] Acquire the second optical detection signal of the second mixture;

[0270] Interference analysis is performed based on the second optical detection signal to obtain the interference level γ of the sample.

[0271] Preferably, the sample is plasma, and the interfering agent includes at least one of lipemia, hemolysis, and jaundice. In some embodiments of this application, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may specifically be used to perform the following steps:

[0272] The second mixture was irradiated with light sources of wavelengths of 405nm, 545nm, 660nm and 800nm.

[0273] In some embodiments of this application, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may specifically perform the following steps:

[0274] The absorbance values ​​of the lipemia in the second mixture at wavelengths of 660 nm and 800 nm were obtained respectively.

[0275] The lipid level in the plasma is calculated and analyzed according to the first formula, which is:

[0276]

[0277] Wherein, ΔAbsL represents the difference in absorbance of lipemia in the second mixture at wavelengths of 660nm and 800nm, C represents the lipemia grade coefficient, and L Index Indicates the level of lipid levels;

[0278] Wherein, γ is the L Index .

[0279] In some embodiments of this application, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may specifically perform the following steps:

[0280] Obtain the absorbance values ​​of the hemolyzed solution in the second mixture at wavelengths of 545 nm and 660 nm.

[0281] The hemolysis level in the plasma was calculated and analyzed according to the second formula, which is:

[0282]

[0283] Wherein, ΔAbs H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, A represents the hemolysis grade coefficient, ΔAbs L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the correction coefficient for lipemia when calculating the hemolysis grade, and H Index Indicates the degree of hemolysis;

[0284] Wherein, γ includes L Index and the H Index .

[0285] In some embodiments of this application, when a computer program stored on a computer-readable storage medium is executed by a processor, the processor may specifically perform the following steps:

[0286] Obtain the absorbance values ​​of jaundice in the second mixture at wavelengths of 405 nm and 660 nm.

[0287] The jaundice level in the plasma was calculated and analyzed according to the third formula, which is:

[0288]

[0289] Wherein, ΔAbs I represents the absorbance difference of jaundice in the second mixture at wavelengths of 405nm and 660nm, F represents the correction factor for hemolysis when calculating jaundice, ΔAbs H represents the absorbance difference of hemolysis in the second mixture at wavelengths of 545nm and 660nm, ΔAbs L represents the absorbance difference of lipemia in the second mixture at wavelengths of 660nm and 800nm, E represents the correction factor for lipemia when calculating the hemolysis grade, G represents the correction factor for lipemia when calculating jaundice, D represents the jaundice grade coefficient, and I Index Indicates the jaundice level;

[0290] Wherein, γ includes L Index The H Index and the I Index .

[0291] Those skilled in the art will clearly understand that, for the sake of convenience and brevity, the specific working processes of the systems, devices, and units described above can be referred to the corresponding processes in the foregoing method embodiments, and will not be repeated here.

[0292] In the several embodiments provided in this application, it should be understood that the disclosed systems, apparatuses, and methods can be implemented in other ways. For example, the apparatus embodiments described above are merely illustrative; for instance, the division of units is only a logical functional division, and in actual implementation, there may be other division methods. For example, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. Furthermore, the coupling or direct coupling or communication connection shown or discussed may be an indirect coupling or communication connection between apparatuses or units through some interfaces, and may be electrical, mechanical, or other forms.

[0293] The units described as separate components may or may not be physically separate. The components shown as units may or may not be physical units; that is, they may be located in one place or distributed across multiple network units. Some or all of the units can be selected to achieve the purpose of this embodiment according to actual needs.

[0294] Furthermore, the functional units in the various embodiments of this application can be integrated into one processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit. The integrated unit can be implemented in hardware or as a software functional unit.

[0295] If the integrated unit is implemented as a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of this application, in essence, or the part that contributes to the prior art, or all or part of the technical solution, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions to cause a computer device (which may be a personal computer, server, or network device, etc.) to execute all or part of the steps of the methods described in the various embodiments of this application. The aforementioned storage medium includes various media capable of storing program code, such as USB flash drives, portable hard drives, read-only memory (ROM), random access memory (RAM), magnetic disks, or optical disks.

[0296] The above-described embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application.

Claims

1. An interference detection method, characterized by, The method comprises the following steps: obtaining the level γ of the interferent in the sample, wherein the level γ of the interferent is used to represent the concentration or level of the interferent in the sample; when the sample is measured by using multiple wavelengths, obtaining the main wavelength interferent level threshold γ1 corresponding to the main wavelength matched with the measurement item and the secondary wavelength interferent level threshold γ2 corresponding to the secondary wavelength matched with the measurement item, wherein γ1< γ2, the main wavelength interferent level threshold refers to the maximum allowed concentration or level of the interferent when the measurement item is measured by using the main wavelength, and the secondary wavelength interferent level threshold refers to the maximum allowed concentration or level of the interferent when the measurement item is measured by using the secondary wavelength; if γ≤ γ1, then the anti-interference measurement of the measurement item is performed on the sample by using the main wavelength; if γ1< γ≤ γ2, then the anti-interference measurement of the measurement item is performed on the sample by using the secondary wavelength; if γ> γ2, then the sample is diluted, and then the anti-interference measurement of the measurement item is performed on the diluted sample by using the main wavelength or the secondary wavelength.

2. The method of claim 1, wherein, The anti-interference measurement of the diluted sample by using the main wavelength or the secondary wavelength comprises the following steps: obtaining the level γ' of the interferent in the diluted sample; if γ'≤ γ1, then the anti-interference measurement of the measurement item is performed on the sample by using the main wavelength; if γ1< γ'≤ γ2, then the anti-interference measurement of the measurement item is performed on the sample by using the secondary wavelength.

3. The method according to claim 1 or 2, characterized in that, The dilution multiple is a fixed value, or the dilution multiple is proportional to the level γ of the interferent in the sample.

4. The method of claim 1, wherein, The method further comprises the following steps: when the sample is measured by using a single wavelength, obtaining the interferent level threshold γ3 corresponding to the single wavelength; if γ≤ γ3, then the measurement item is measured on the sample by using the single wavelength.

5. The method of claim 4, wherein, The method further comprises the following steps: if γ> γ3, then the sample is diluted; obtaining the level γ' of the interferent in the diluted sample; if γ'≤ γ3, then the anti-interference measurement of the measurement item is performed on the sample by using the single wavelength.

6. The method of claim 1, wherein, The method for obtaining the level γ of the interferent in the sample comprises the following steps: sucking the sample to be detected; adding part of the sample into a second container to prepare a second mixed solution of the sample and a diluent; irradiating the second mixed solution by using a multi-wavelength light source; collecting a second optical detection signal of the second mixed solution; performing interferent analysis according to the second optical detection signal to obtain the level γ of the interferent in the sample.

7. The method of claim 6, wherein, The sample is blood plasma, and the interferent comprises at least one of lipemia, hemolysis and jaundice. The method for irradiating the second mixed solution by using a multi-wavelength light source comprises the following steps: irradiating the second mixed solution by using light sources with wavelengths of 405 nm, 545 nm, 660 nm and 800 nm.

8. The method of claim 7, wherein, The method for performing interferent analysis according to the second optical detection signal to obtain the level γ of the interferent in the sample comprises the following steps: respectively obtaining the absorbance values of lipemia in the second mixed solution at wavelengths of 660 nm and 800 nm; performing calculation analysis on the level of lipemia in the blood plasma according to a first formula, wherein the first formula is: wherein ΔAbs L represents the absorbance difference of the lipemia in the second mixed solution at 660 nm and 800 nm wavelengths, C represents a lipemia grade coefficient, and L Index represents the lipemia grade. wherein the γ is the L Index .

9. The method of claim 8, wherein, The interference analysis according to the second optical detection signal to obtain the interference level γ of the sample further comprises: obtaining the absorbance values of hemolysis in the second mixed solution at 545 nm and 660 nm wavelengths; calculating and analyzing the hemolysis level in the plasma according to a second formula, the second formula being: wherein ΔAbsH represents the absorbance difference at 545 nm and 660 nm wavelengths of hemolysis in the second mixture, A represents the hemolysis grade coefficient, ΔAbsL represents the absorbance difference at 660 nm and 800 nm wavelengths of lipemia in the second mixture, E represents the correction coefficient of lipemia when calculating the hemolysis grade, H Index represents the hemolysis grade; wherein the γ comprises the L Index and the H Index .

10. The method of claim 9, wherein, The interference analysis according to the second optical detection signal to obtain the interference level γ of the sample further comprises: obtaining the absorbance values of jaundice in the second mixed solution at 405 nm and 660 nm wavelengths; calculating and analyzing the jaundice level in the plasma according to a third formula, the third formula being: wherein ΔAbs I represents the absorbance difference at 405 nm and 660 nm wavelengths of jaundice in the second mixed solution, F represents a correction coefficient of hemolysis when calculating jaundice, ΔAbs H represents the absorbance difference at 545 nm and 660 nm wavelengths of hemolysis in the second mixed solution, ΔAbs L represents the absorbance difference at 660 nm and 800 nm wavelengths of lipemia in the second mixed solution, E represents a correction coefficient of lipemia when calculating the degree of hemolysis, G represents a correction coefficient of lipemia when calculating jaundice, D represents a degree coefficient of jaundice, I Index represents the degree of jaundice; wherein the γ comprises the L Index , the H Index , and the I Index .

11. A sample analyzer characterized by, comprising: a first container for carrying a first mixed solution prepared from part of the sample and a detection reagent; a second container for carrying a second mixed solution prepared from another part of the sample and a diluent; an optical device for irradiating the first mixed solution and the second mixed solution with a multi-wavelength light source; a signal acquisition device for acquiring a first optical detection signal of the first mixed solution and a second optical detection signal of the second mixed solution; a processor for: obtaining the interference level γ of the sample according to the second optical detection signal, the interference level γ being used to represent the interference concentration or level of the sample; when performing item measurement on the sample with multiple wavelengths, obtaining a main wavelength interference level threshold γ1 corresponding to a main wavelength matched with the measurement item and a secondary wavelength interference level threshold γ2 corresponding to a secondary wavelength matched with the measurement item, wherein γ1< γ2, the main wavelength interference level threshold being the maximum interference concentration or level allowed when performing the measurement item measurement on the sample with the main wavelength, and the secondary wavelength interference level threshold being the maximum interference concentration or level allowed when performing the measurement item measurement on the sample with the secondary wavelength; if γ≤ γ1, performing anti-interference measurement on the sample with the main wavelength; if γ1< γ≤ γ2, performing anti-interference measurement on the sample with the secondary wavelength; if γ> γ2, diluting the sample, and then performing anti-interference measurement on the diluted sample with the main wavelength or the secondary wavelength.

12. The sample analyzer of claim 11, wherein, The processor is specifically configured to: obtain the interference level γ' of the diluted sample; if γ'≤ γ1, perform anti-interference measurement on the sample with the main wavelength; if γ1< γ'≤ γ2, perform anti-interference measurement on the sample with the secondary wavelength.

13. The sample analyzer of claim 11 or 12, wherein, The dilution multiple is a fixed value, or the dilution multiple is proportional to the interference level γ of the sample.

14. The sample analyzer of claim 11, wherein, The processor is further configured to: when performing item measurement on the sample with a single wavelength, obtain an interference level threshold γ3 corresponding to the single wavelength; if γ≤ γ3, perform item measurement on the sample with the single wavelength.

15. The sample analyzer of claim 14, wherein, The processor is further configured to: if γ> γ3, dilute the sample; obtain the interference level γ' of the diluted sample; If γ' ≤ γ3, an anti-interference measurement is performed on the sample using the single wavelength.

16. The sample analyzer of claim 11, wherein, The processor is specifically configured to: Perform interference analysis according to the second optical detection signal to obtain the level γ of the interference of the sample.

17. The sample analyzer of claim 16, wherein, The sample is blood plasma, and the interference includes at least one of lipemia, hemolysis and jaundice. The processor is specifically configured to: Irradiate the second mixed solution by using light sources with wavelengths of 405 nm, 545 nm, 660 nm and 800 nm.

18. The sample analyzer of claim 17, wherein, The processor is specifically configured to: Obtain the absorbance values of lipemia in the second mixed solution at wavelengths of 660 nm and 800 nm, respectively. Perform calculation and analysis on the level of lipemia in the blood plasma according to a first formula, the first formula being: wherein ΔAbs L represents the difference in absorbance of the lipemia at 660 nm and 800 nm wavelengths, C represents the lipemia grade coefficient, and L Index represents the lipemia grade; wherein the γ is the L Index .

19. The sample analyzer of claim 18, wherein, The processor is further configured to: Obtain the absorbance values of hemolysis in the second mixed solution at wavelengths of 545 nm and 660 nm. Perform calculation and analysis on the level of hemolysis in the blood plasma according to a second formula, the second formula being: wherein ΔAbsH represents the absorbance difference of hemolysis at 545 nm and 660 nm wavelengths, A represents the hemolysis grade coefficient, ΔAbsL represents the absorbance difference of lipemia at 660 nm and 800 nm wavelengths, E represents the correction coefficient of lipemia when calculating the hemolysis grade, H Index represents the hemolysis grade; wherein the γ comprises the L Index and the H Index .

20. The sample analyzer of claim 19, wherein, The processor is further configured to: Obtain the absorbance values of jaundice in the second mixed solution at wavelengths of 405 nm and 660 nm. Perform calculation and analysis on the level of jaundice in the blood plasma according to a third formula, the third formula being: wherein ΔAbs I represents the absorbance difference at 405 nm and 660 nm wavelengths of jaundice in the second mixed solution, F represents a correction coefficient of hemolysis when calculating jaundice, ΔAbs H represents the absorbance difference at 545 nm and 660 nm wavelengths of hemolysis in the second mixed solution, ΔAbs L represents the absorbance difference at 660 nm and 800 nm wavelengths of lipemia in the second mixed solution, E represents a correction coefficient of lipemia when calculating the degree of hemolysis, G represents a correction coefficient of lipemia when calculating jaundice, D represents a degree coefficient of jaundice, I Index represents the degree of jaundice; wherein the γ comprises the L Index , the H Index , and the I Index .

21. The sample analyzer of any one of claims 11 to 20, wherein, The sample analyzer is a coagulation analyzer.

22. A computer readable storage medium having stored thereon a computer program, characterized in that, The computer program, when executed by the processor, is configured to implement the anti-interference detection method according to any one of claims 1 to 10.

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